ES2149706B1 - Promotores de los genes glutamato deshidrogenasa, beta-n-acetilhexosaminidas y gamma-actina y su utilizacion en sistemas de expresion, secrecion y antisentido de hongos filamentosos. - Google Patents

Promotores de los genes glutamato deshidrogenasa, beta-n-acetilhexosaminidas y gamma-actina y su utilizacion en sistemas de expresion, secrecion y antisentido de hongos filamentosos.

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Publication number
ES2149706B1
ES2149706B1 ES009801858A ES9801858A ES2149706B1 ES 2149706 B1 ES2149706 B1 ES 2149706B1 ES 009801858 A ES009801858 A ES 009801858A ES 9801858 A ES9801858 A ES 9801858A ES 2149706 B1 ES2149706 B1 ES 2149706B1
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Spain
Prior art keywords
promoters
expression
genes
actin
secretion
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
ES009801858A
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English (en)
Other versions
ES2149706A1 (es
Inventor
Fuente Jose Luis Barredo
Saiz Marta Rodriguez
Valle Miguel Angel Moreno
De La Vieja Alfonso J Collados
Maldonado Francisco Salto
Garcia Bruno Diez
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Antibioticos SA
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Antibioticos SA
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Publication date
Application filed by Antibioticos SA filed Critical Antibioticos SA
Publication of ES2149706A1 publication Critical patent/ES2149706A1/es
Application granted granted Critical
Publication of ES2149706B1 publication Critical patent/ES2149706B1/es
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1137—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0014—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4)
    • C12N9/0016—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4) with NAD or NADP as acceptor (1.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y104/00—Oxidoreductases acting on the CH-NH2 group of donors (1.4)
    • C12Y104/01—Oxidoreductases acting on the CH-NH2 group of donors (1.4) with NAD+ or NADP+ as acceptor (1.4.1)
    • C12Y104/01002—Glutamate dehydrogenase (1.4.1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01052—Beta-N-acetylhexosaminidase (3.2.1.52)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Virology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Promotores de los genes glutamato deshidrogenasa, {b -N-acetilhexosaminidasa y {y -actina y su utilización en sistemas de expresión, secreción y antisentido de hongos filamentosos. Se describe la utilización de los promotores de los genes que codifican: (I) glutamato deshidrogenasa NADP dependiente (EC.1.4.1.4) de Penicillium chrysogenum, (II) {b -N-acetilhexosa-minidasa (EC.3.2.1.52) de Penicillium chrysogenum y (III) {y -actina de Penicillium chrysogenum y Acremonium chrysogenum, los cuales pueden ser utilizados para la construcción de potentes vectores de expresión y secreción útiles tanto para P. chrysogenum como para A. chrysogenum y especies relacionadas. Asimismo, estos promotores pueden utilizarse para el bloqueo de la expresión génica mediante construcciones antisentido. Bajo el control de los promotores anteriormente citados se puede dirigir la expresión de otros genes en hongos filamentosos, incrementándose la producción de antibióticos y/o proteínas inherentes a los mismos.
ES009801858A 1997-03-05 1998-09-03 Promotores de los genes glutamato deshidrogenasa, beta-n-acetilhexosaminidas y gamma-actina y su utilizacion en sistemas de expresion, secrecion y antisentido de hongos filamentosos. Expired - Fee Related ES2149706B1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES009700482A ES2127697B1 (es) 1997-03-05 1997-03-05 Promotores de los genes glutamato deshidrogenasa, beta-n-acetilhexosaminidasa y gamma-actina y su utilizacion en sistemas de expresion, secrecion y antisentido de hongos filamentosos.

Publications (2)

Publication Number Publication Date
ES2149706A1 ES2149706A1 (es) 2000-11-01
ES2149706B1 true ES2149706B1 (es) 2001-05-16

Family

ID=8298517

Family Applications (3)

Application Number Title Priority Date Filing Date
ES009700482A Expired - Fee Related ES2127697B1 (es) 1997-03-05 1997-03-05 Promotores de los genes glutamato deshidrogenasa, beta-n-acetilhexosaminidasa y gamma-actina y su utilizacion en sistemas de expresion, secrecion y antisentido de hongos filamentosos.
ES009801858A Expired - Fee Related ES2149706B1 (es) 1997-03-05 1998-09-03 Promotores de los genes glutamato deshidrogenasa, beta-n-acetilhexosaminidas y gamma-actina y su utilizacion en sistemas de expresion, secrecion y antisentido de hongos filamentosos.
ES009801859A Expired - Fee Related ES2149707B1 (es) 1997-03-05 1998-09-03 Promotores de los genes glutamato deshidrogenasa, beta-n-acetilhexosa,omodasa y gamma-actina y su utilizacion en sistemas de expresion, secrecion y antisentido de hongos filamentosos.

Family Applications Before (1)

Application Number Title Priority Date Filing Date
ES009700482A Expired - Fee Related ES2127697B1 (es) 1997-03-05 1997-03-05 Promotores de los genes glutamato deshidrogenasa, beta-n-acetilhexosaminidasa y gamma-actina y su utilizacion en sistemas de expresion, secrecion y antisentido de hongos filamentosos.

Family Applications After (1)

Application Number Title Priority Date Filing Date
ES009801859A Expired - Fee Related ES2149707B1 (es) 1997-03-05 1998-09-03 Promotores de los genes glutamato deshidrogenasa, beta-n-acetilhexosa,omodasa y gamma-actina y su utilizacion en sistemas de expresion, secrecion y antisentido de hongos filamentosos.

Country Status (17)

Country Link
US (2) US6300095B1 (es)
JP (1) JP2000509613A (es)
KR (1) KR20000065202A (es)
CN (1) CN1219200A (es)
AU (1) AU6101198A (es)
CA (1) CA2253250A1 (es)
CZ (1) CZ353598A3 (es)
EE (1) EE9800381A (es)
ES (3) ES2127697B1 (es)
HU (1) HUP0000870A3 (es)
IL (1) IL126647A0 (es)
LT (1) LT4557B (es)
LV (1) LV12264B (es)
PL (1) PL329722A1 (es)
SK (1) SK149598A3 (es)
WO (1) WO1998039459A1 (es)
ZA (1) ZA981896B (es)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6610839B1 (en) * 1997-08-14 2003-08-26 Geron Corporation Promoter for telomerase reverse transcriptase
US6777203B1 (en) * 1997-11-19 2004-08-17 Geron Corporation Telomerase promoter driving expression of therapeutic gene sequences
ES2127697B1 (es) * 1997-03-05 2000-03-16 Antibioticos Sau Promotores de los genes glutamato deshidrogenasa, beta-n-acetilhexosaminidasa y gamma-actina y su utilizacion en sistemas de expresion, secrecion y antisentido de hongos filamentosos.
US7378244B2 (en) * 1997-10-01 2008-05-27 Geron Corporation Telomerase promoters sequences for screening telomerase modulators
ES2143408B1 (es) * 1998-04-02 2000-12-01 Urquima Sa Promotor y construcciones para expresion de proteinas recombinantes en hongos filamentosos.
WO2000052176A1 (de) * 1999-03-03 2000-09-08 Cilian Ag β-HEXOSAMINIDASE SOWIE DIESE KODIERENDE DNA-SEQUENZ AUS CILIATEN UND DEREN VERWENDUNG
US20090104165A1 (en) * 2004-09-22 2009-04-23 Bioworks, Inc. Transgenic strains of trichoderma and their use in biocontrol
EP1801221A1 (en) * 2005-12-22 2007-06-27 Sandoz AG Promoter sequences
CN102127546B (zh) * 2010-11-24 2012-10-24 山东农业大学 一种骨骼肌特异性actin启动子及其应用
CN116004892B (zh) * 2022-10-31 2025-04-29 中国热带农业科学院环境与植物保护研究所 一种香蕉灰纹病菌的pcr检测引物及检测方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8508800D0 (en) * 1985-04-04 1985-05-09 Glaxo Group Ltd Microbiological process
FI864473L (fi) * 1985-11-20 1987-05-21 Panlabs Inc Sammansatta yttryckskassetter foer fungaltransformering.
ES2127697B1 (es) * 1997-03-05 2000-03-16 Antibioticos Sau Promotores de los genes glutamato deshidrogenasa, beta-n-acetilhexosaminidasa y gamma-actina y su utilizacion en sistemas de expresion, secrecion y antisentido de hongos filamentosos.

Also Published As

Publication number Publication date
US6300095B1 (en) 2001-10-09
KR20000065202A (ko) 2000-11-06
LT98152A (en) 1999-06-25
ES2149707A1 (es) 2000-11-01
LT4557B (lt) 1999-10-25
CZ353598A3 (cs) 1999-02-17
ES2149706A1 (es) 2000-11-01
US6558921B1 (en) 2003-05-06
EE9800381A (et) 1999-04-15
ZA981896B (en) 1998-09-14
CN1219200A (zh) 1999-06-09
PL329722A1 (en) 1999-04-12
LV12264A (lv) 1999-04-20
AU6101198A (en) 1998-09-22
HUP0000870A3 (en) 2001-09-28
IL126647A0 (en) 1999-08-17
WO1998039459A1 (es) 1998-09-11
ES2149707B1 (es) 2001-05-16
LV12264B (en) 1999-10-20
ES2127697A1 (es) 1999-04-16
SK149598A3 (en) 2000-03-13
ES2127697B1 (es) 2000-03-16
HUP0000870A2 (en) 2000-07-28
CA2253250A1 (en) 1998-09-11
JP2000509613A (ja) 2000-08-02

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