ES2173237T3 - Procedimiento de fabricacion de anticuerpos monoclonales humanos y su uso. - Google Patents

Procedimiento de fabricacion de anticuerpos monoclonales humanos y su uso.

Info

Publication number
ES2173237T3
ES2173237T3 ES96117361T ES96117361T ES2173237T3 ES 2173237 T3 ES2173237 T3 ES 2173237T3 ES 96117361 T ES96117361 T ES 96117361T ES 96117361 T ES96117361 T ES 96117361T ES 2173237 T3 ES2173237 T3 ES 2173237T3
Authority
ES
Spain
Prior art keywords
monoclonal antibodies
human monoclonal
manufacturing procedure
antibody
switched
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES96117361T
Other languages
English (en)
Inventor
Georg Wilhelm Prof Bornkamm
Dirk Priv Doz Dr Eick
Bettina Dr Kempkes
Nicola Maria Jochner
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Helmholtz Zentrum München
Original Assignee
Helmholtz Zentrum München
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Helmholtz Zentrum München filed Critical Helmholtz Zentrum München
Application granted granted Critical
Publication of ES2173237T3 publication Critical patent/ES2173237T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

DE ACUERDO CON LA INVENCION SE PREPARA UN PROCEDIMIENTO PARA LA OBTENCION DE ANTICUERPOS MONOCLONALES HUMANOS CON LAS SIGUIENTES ETAPAS: A) INMORTALIZACION DE CELULAS B QUE PRODUCEN ANTICUERPOS HUMANOS CON UN VIRUS DE EPSTEIN-BARR O UN DERIVADO DE EL, B) ESCRUTINIO EN LAS CELULAS INMORTALES DE SU ESPECIFICIDAD PARA EL ANTIGENO DESEADO Y AISLAMIENTO DE CLONES CELULARES, QUE PRODUCEN LOS CORRESPONDIENTES ANTICUERPOS, C) RECUPERACION DE LOS ANTICUERPOS DESEADOS DE LOS CULTIVOS; ESTE PROCEDIMIENTO SE CARACTERIZA PORQUE D) SE UTILIZA UN VIRUS DE EPSTEIN-BARR O UN DERIVADO DE EL CON UN GEN CONDICIONAL DEL ANTIGENO 2 NUCLEAR DEL VIRUS DE EPSTEINBARR (EBNA2) O UN DERIVADO DE EL, EN UNION FUNCIONAL, ESTE GEN SE CONECTA AL MENOS PARCIALMENTE PARA LA INMORTALIZACION, Y E) EL GEN EBNA2 O SU DERIVADO SE DESCONECTA AL MENOS PARCIALMENTE ANTES DE RECUPERAR EL ANTICUERPO.
ES96117361T 1995-11-09 1996-10-29 Procedimiento de fabricacion de anticuerpos monoclonales humanos y su uso. Expired - Lifetime ES2173237T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19541844A DE19541844C1 (de) 1995-11-09 1995-11-09 Verfahren zur Herstellung von menschlichen Antikörpern und deren Verwendung

Publications (1)

Publication Number Publication Date
ES2173237T3 true ES2173237T3 (es) 2002-10-16

Family

ID=7777063

Family Applications (1)

Application Number Title Priority Date Filing Date
ES96117361T Expired - Lifetime ES2173237T3 (es) 1995-11-09 1996-10-29 Procedimiento de fabricacion de anticuerpos monoclonales humanos y su uso.

Country Status (6)

Country Link
US (1) US5798230A (es)
EP (1) EP0773228B1 (es)
AT (1) ATE215563T1 (es)
DE (2) DE19541844C1 (es)
DK (1) DK0773228T3 (es)
ES (1) ES2173237T3 (es)

Families Citing this family (64)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19740571C1 (de) * 1997-09-15 1999-03-18 Gsf Forschungszentrum Umwelt Verfahren zur Stimulation von T-Zellen mit gewünschter Antigenspezifität
DE19816116C2 (de) * 1998-04-09 2000-06-29 Gsf Forschungszentrum Umwelt Verfahren zur Immortalisation von Zellen mit Hilfe konditional transformierter Helferzellen
ES2319831T3 (es) 1998-09-14 2009-05-12 Board Of Regents, The University Of Texas System Metodos de tratamiento de mieloma multiple y resorcion osea inducida por mieloma usando antagonistas de la union receptor/integrina.
JP2002538167A (ja) 1999-03-03 2002-11-12 バイオジェン インコーポレイテッド 脂質の代謝および貯蔵を調節する方法
ES2243259T3 (es) * 1999-04-22 2005-12-01 Biogen Idec Ma Inc. Metodo para tratar la fibrosis utilizando un antagonista de la subunidad alfa 4 de la integrina.
UA73300C2 (en) * 1999-06-01 2005-07-15 Biogen Inc Use of composition containing homolog of antibody antagonizing interaction between integrin with alpha-4 subunit and its ligand for treating fibrosis
EP1181054A1 (en) 1999-06-01 2002-02-27 Biogen, Inc. A blocking monoclonal antibody to vla-1 and its use for the treatment of inflammatory disorders
US7241443B1 (en) * 1999-07-15 2007-07-10 Camas Incorporated Immunogen adherence inhibitor and method of making and using same
AU783110B2 (en) 1999-12-16 2005-09-22 Biogen Ma Inc. Methods of treating central nervous system ischemic or hemorrhagic injury using anti alpha4 integrin antagonists
EP1423431B1 (en) * 2001-04-13 2010-06-16 Biogen Idec MA Inc. Antibodies to vla-1
ES2384960T3 (es) 2001-12-18 2012-07-16 Cancer Research Technology Limited Procedimiento para la generación de líneas celulares proliferativas y que se diferencian
GB2387599B (en) * 2002-04-17 2005-08-10 Jason Peter Brown Methods for producing antibodies
JP2007521249A (ja) 2003-01-24 2007-08-02 エラン ファーマシューティカルズ，インコーポレイテッド 脱髄疾患および麻痺のための組成物ならびに再有髄化剤を投与することによるそれらの治療
GB2398783A (en) 2003-02-26 2004-09-01 Antonio Lanzavecchia A method for producing immortalised human B memory lymphocytes
EP1626991A2 (en) * 2003-05-23 2006-02-22 Morphotek, Inc. Anti alpha-folate-receptor-tetramer antibodies
DK1639014T3 (da) 2003-06-13 2011-01-17 Biogen Idec Inc Aglycosyl-anti-CD154 (CD40-ligand)-antistoffer og anvendelser deraf
WO2005056599A2 (en) * 2003-12-08 2005-06-23 Morphotek, Inc. Antibodies that specifically bind pms2
US20090113563A1 (en) * 2004-02-12 2009-04-30 Yoshio Umezawa Probe for detecting nuclear receptor agonist or antagonist and method for screening agonist or antagonist to nuclear receptor with the use of the same
CA2556027C (en) 2004-02-12 2015-11-24 Morphotek, Inc. Monoclonal antibodies that specifically block biological activity of a tumor antigen
AU2005287406B2 (en) 2004-07-26 2011-08-18 Biogen Ma Inc. Anti-CD154 antibodies
CA2478458A1 (en) * 2004-08-20 2006-02-20 Michael Panzara Treatment of pediatric multiple sclerosis
CA2587597A1 (en) 2004-11-19 2006-05-26 Biogen Idec Ma Inc. Treatment for multiple sclerosis
NZ581497A (en) * 2004-12-03 2012-07-27 Biogen Idec Inc Delaying or preventing onset of multiple sclerosis by vla-4 ginding antibody
US7517870B2 (en) 2004-12-03 2009-04-14 Fondazione Telethon Use of compounds that interfere with the hedgehog signaling pathway for the manufacture of a medicament for preventing, inhibiting, and/or reversing ocular diseases related with ocular neovascularization
ATE476449T1 (de) * 2004-12-03 2010-08-15 Morphotek Inc Verwendung von endosialinbindenden proteinen zum isolieren endosialinpositiver zellen
AU2006214179A1 (en) 2005-02-17 2006-08-24 Biogen Idec Ma Inc. Treating neurological disorders
EP1865985B1 (en) 2005-03-02 2010-08-25 Biogen Idec MA Inc. Kim-1 antibodies for treatment of th2-mediated conditions
EP2322560B1 (en) 2005-03-10 2013-09-04 Morphotek, Inc. Anti-mesothelin antibodies
CN101189023B (zh) 2005-03-31 2013-01-30 通用医疗公司 监测和调制hgf/hgfr活性
US8124350B2 (en) 2005-04-04 2012-02-28 Biogen Idec Ma Inc. Methods and products for evaluating an immune response to a therapeutic protein
US20060230734A1 (en) * 2005-04-14 2006-10-19 Deere & Company, A Delaware Corporation Twin belt mule drive
EP2172487A1 (en) 2005-04-22 2010-04-07 Morphotek Inc. Antibodies with Immune Effector Activity that Internalize in Folate Receptor Alpha-Positive Cells
AU2006241235B2 (en) * 2005-04-22 2012-04-05 Eisai, Inc. Antibodies with immune effector activity and that internalize in endosialin-positive cells
JP2009523807A (ja) 2006-01-18 2009-06-25 ザ ジェネラル ホスピタル コーポレイション リンパ機能を高める方法
JP2009534297A (ja) 2006-03-10 2009-09-24 ザイモジェネティクス， インコーポレイテッド Ｉｌ−１７ａおよびｉｌ−１７ｆの両方に結合する抗体ならびにその使用方法
DE202006007499U1 (de) * 2006-05-11 2007-01-11 Osaka University Verwendung einer Zusammensetzung, die Plexin-A1-DAP12-Interaktion inhibiert, zur Behandlung von Entzündungs-, Autoimmun- oder Knochenresorptionserkrankungen
DE202006007590U1 (de) * 2006-05-12 2006-12-14 Osaka University Verwendung einer Zusammensetzung, die Sema7A- und VLA-1-Interaktion inhibiert, zum Behandeln von Cytokin-vermittelten Erkrankungen
CA2652815C (en) 2006-05-25 2015-12-15 Biogen Idec Ma Inc. Methods of treating stroke
EP2056709A4 (en) 2006-08-11 2013-05-01 Univ New Jersey Med DOUBLE-SENSITIZER WITH LUMINESCENCE COMPOUNDS, CONJUGATES AND USE
US8288110B2 (en) * 2006-12-04 2012-10-16 Perkinelmer Health Sciences, Inc. Biomarkers for detecting cancer
EP2134855A4 (en) 2007-03-12 2011-01-05 Dana Farber Cancer Inst Inc USES OF FANCI AND FANCI MODULATING AGENTS IN PROGNOSIS, DIAGNOSIS AND CANCER THERAPY
CA2680613A1 (en) * 2007-03-13 2008-09-18 National Jewish Medical And Research Center Methods for generation of antibodies
BRPI0809042B1 (pt) 2007-03-22 2021-08-31 Biogen Ma Inc. Proteína de ligação a cd154 isolada, seu uso, e composição
RS55447B1 (sr) 2007-04-05 2017-04-28 Morphotek Inc Metode za inhibiranje vezivanja endosijalina za ligande
AU2007352412B2 (en) 2007-04-27 2013-05-16 Zymogenetics, Inc. Antibodies that bind both IL-17A and IL-17F and methods of using the same
ES2465223T3 (es) 2007-04-27 2014-06-05 Zymogenetics, Inc. Antagonistas de IL-17A, IL-17F e IL-23P19 y procedimientos de uso
CA2690382A1 (en) 2007-06-14 2008-12-24 Biogen Idec Ma Inc. Antibody formulations
WO2009020923A1 (en) 2007-08-03 2009-02-12 Musc Foundation For Research Development Human monoclonal antibodies and methods for producing the same
WO2009026075A1 (en) 2007-08-16 2009-02-26 Irm Llc Methods and compositions for treating cancers
EP2257572A1 (en) * 2008-03-27 2010-12-08 The Government of the United States of America as represented by The Secretary of the Department of Health and Human Services Human anti-mesothelin monoclonal antibodies
WO2009124109A1 (en) * 2008-04-04 2009-10-08 The Government Of The U.S. A. As Represented By The Secretary Of The Dept. Of Health &Human Services Human monoclonal antibodies specific for cd22
CA2726804C (en) 2008-06-02 2021-12-28 Dana-Farber Cancer Institute, Inc. Xbp1, cd138, and cs1 peptides
MX2011010971A (es) * 2009-04-17 2012-01-19 Biogen Idec Inc Composiciones y metodos para tratar leucemia mielogena aguda.
EP2485762B1 (en) 2009-10-11 2017-12-13 Biogen MA Inc. Anti-vla-4 related assays
WO2011088193A2 (en) 2010-01-13 2011-07-21 University Of Medicine And Dentistry Of New Jersey Fluorophore chelated lanthanide luminiscent probes with improved quantum efficiency
ES2912934T3 (es) 2010-04-16 2022-05-30 Biogen Ma Inc Anticuerpos anti VLA-4
WO2012027494A1 (en) 2010-08-24 2012-03-01 Regents Of The University Of Minnesota Bispecific targeting reagents
EP2614084A4 (en) 2010-09-09 2014-02-19 Purdue Research Foundation HUMANE FOLAT RECEPTOR BETA ANTIBODIES AND METHOD FOR THEIR USE
EP2724157B1 (en) 2011-06-27 2017-03-08 Valneva Method for screening cells
WO2013123114A2 (en) 2012-02-16 2013-08-22 Santarus, Inc. Antibody formulations
EP3575311A1 (en) 2012-03-20 2019-12-04 Biogen MA Inc. Jcv neutralizing antibodies
CN103196817A (zh) * 2013-04-10 2013-07-10 哈尔滨体育学院 一种优秀冰雪运动员eb病毒转化细胞凋亡鉴定方法
WO2018140510A1 (en) 2017-01-25 2018-08-02 Biogen Ma Inc. Compositions and methods for treatment of stroke and other cns disorders
CN112867394B9 (zh) 2018-06-04 2024-12-06 马萨诸塞州渤健公司 具有降低的效应功能的抗vla-4抗体

Also Published As

Publication number Publication date
DK0773228T3 (da) 2002-07-22
ATE215563T1 (de) 2002-04-15
DE59609000D1 (de) 2002-05-08
EP0773228A1 (de) 1997-05-14
EP0773228B1 (de) 2002-04-03
DE19541844C1 (de) 1997-07-24
US5798230A (en) 1998-08-25

Similar Documents

Publication Publication Date Title
DK0773228T3 (da) Fremgangsmåde til fremstilling af monoklonale human-antistoffer og deres anvendelse
NO803658L (no) Hybridcellelinje for fremstilling av komplementfikserende monoklonalt antistoff til humane undertrykker t-celler, selve antistoffet og fremgangsmaater for dets fremstilling
IL132468A0 (en) A kit for inducing immune response against a tumor or a disease caused by an infection agent
ES2188583T3 (es) Polipeptidos para el virus de la hepatitis c (hcv).
DK0633929T3 (da) Fremgangsmåde til in vitro proliferation af dendritcelleforstadier og deres anvendelse til at producere immunogener
FR12C0004I2 (fr) Anticorps contre l'antigene ctla-4 humain et utilisation
DK180580A (da) Hybrid cellelinie og fremgangsmaade til fremstilling og anvendelse deraf
ES2139607T3 (es) Anticuerpos monoclonales contra las proteinas e2/ns1 supuestas vhc y procedimientos de utilizacion de estos anticuerpos.
ATE13317T1 (de) Hybridzellinie zur herstellung monoklonaler antikoerper gegen ein menschliches prothymozytantigen; antikoerper und verfahren zu seiner herstellung; diagnostische und therapeutische verwendungen und diesen antikoerper enthaltende pharmazeutische zusammensetzungen.
BR9610802A (pt) Método para melhoria da resposta imunológica método de preparo de células t de antígeno específico método para induzir tolerância de tecido de enxerto auxiliar de vacina meio de expansão e preparação de células dentríticas população de células dentríticas e respectivo método de preparo método para direcionar células-tronco hematopoiéticas ou células progenitoras a uma linhagem de células dentríticas
DK0853633T3 (da) Humant monoklonalt antistof imod hepatitis B virus overfladeantigen (HBVsAg)
ES2130121T3 (es) Antigeno intra-acrosomico de esperma humano para uso en una vacuna anticonceptiva.
PT912611E (pt) Anticorpos monoclonais humanos para o anti-gene de superficie da hepatite b
ES2072928T3 (es) Antigenos de celulas de islotes pancreaticos obtenidos por clonaje molecular.
ES2126118T3 (es) Inmortalizacion de celulas dendriticas con el oncogen v-myc.
ATE244762T1 (de) Säugetier - expressionssysteme für hüllenprotingene des hepatitit - c - virus
ATE12660T1 (de) Hybridzellinie zur herstellung monoklonaler antikoerper gegen ein menschliches thymozytantigen, antikoerper und verfahren zu seiner herstellung, diagnostische und therapeutische verwendungen und diesen antikoerper enthaltende pharmazeutische zusammensetzungen.
ES2064349T3 (es) Inmunoglobulinas humanas especificas del antigeno relacionado con el cancer e hibridomas humanos-humanos que pueden producir estas inmunoglobulinas humanas.
ES8707114A1 (es) Procedimiento para la obtencion de un anticuerpo monoclonal anti-alfa-amilasa
DK1002092T3 (da) Mimotoper af hypervariabel region 1 af E2-glycoproteinet fra HCV og anvendelser deraf
ES2118129T3 (es) Anticuerpos monoclonales contra las regiones de cubierta putativas del virus vhc y procedimientos de utilizacion de estos.
NO972522L (no) Monoklonale antistoffer med immunoundertrykkende aktivitet
ATE494377T1 (de) Vakzine gegen ipnv die aus hefezellen isoliert werden
WO2002008292A3 (en) Human monoclonal antibody against hepatitis c virus e2 glycoprotein
ES8701837A1 (es) Procedimiento para producir una composicion viral ateunada viva uniforme de hepatitis a.

Legal Events

Date Code Title Description
FG2A Definitive protection

Ref document number: 773228

Country of ref document: ES