ES2179339T3 - Identificacion de variaciones numericas en el adn de celulas. - Google Patents
Identificacion de variaciones numericas en el adn de celulas.Info
- Publication number
- ES2179339T3 ES2179339T3 ES97923750T ES97923750T ES2179339T3 ES 2179339 T3 ES2179339 T3 ES 2179339T3 ES 97923750 T ES97923750 T ES 97923750T ES 97923750 T ES97923750 T ES 97923750T ES 2179339 T3 ES2179339 T3 ES 2179339T3
- Authority
- ES
- Spain
- Prior art keywords
- dna
- identification
- procedure
- cell dna
- cells
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6841—In situ hybridisation
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Immunology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
LA PRESENTE INVENCION TRATA DE UN PROCEDIMIENTO PARA LA IDENTIFICACION DE CAMBIOS NUMERICOS EN EL DNA DE CELULAS, QUE COMPRENDE LAS SIGUIENTES ETAPAS: (A) AISLAMIENTO DEL DNA A PARTIR DE CELULAS NORMALES Y AMPLIFICACION DEL DNA MEDIANTE UN PROCEDIMIENTO DE REACCION EN CADENA DE POLIMERASA (PCR) UTILIZANDO UN INICIADOR TAG, (B) HIBRIDACION IN SITU DE LAS CELULAS QUE SE VAN A INVESTIGAR CON EL DNA AMPLIADO DE (A), (C) AMPLIFICACION DEL DNA PROCEDENTE DE LAS CELULAS HIBRIDADAS IN SITU DE (B) MEDIANTE UN PROCEDIMIENTO DE PCR UTILIZANDO EL INICIADOR TAG DE (A), Y (D) IDENTIFICACION DE LOS CAMBIOS NUMERICOS EN EL DNA AMPLIADO DE (C) DE MANERA CONOCIDA. TAMBIEN TRATA LA INVENCION DE UN EQUIPO PARA LLEVAR A CABO EL PROCEDIMIENTO.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19616381A DE19616381C1 (de) | 1996-04-24 | 1996-04-24 | Nachweis numerischer Veränderungen in der DNA von Zellen |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2179339T3 true ES2179339T3 (es) | 2003-01-16 |
Family
ID=7792317
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES97923750T Expired - Lifetime ES2179339T3 (es) | 1996-04-24 | 1997-04-23 | Identificacion de variaciones numericas en el adn de celulas. |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US20020137029A1 (es) |
| EP (1) | EP0910665B1 (es) |
| JP (1) | JP2000509266A (es) |
| AT (1) | ATE219793T1 (es) |
| DE (2) | DE19616381C1 (es) |
| DK (1) | DK0910665T3 (es) |
| ES (1) | ES2179339T3 (es) |
| WO (1) | WO1997040185A1 (es) |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5015570A (en) * | 1988-05-13 | 1991-05-14 | Molecular Therapeutics, Inc. | Molecular diagnosis of Alzheimer Disease |
| US7229755B1 (en) * | 1993-11-17 | 2007-06-12 | Dana Farber Cancer Institute, Inc. | Method for detection of alterations in the DNA mismatch repair pathway |
-
1996
- 1996-04-24 DE DE19616381A patent/DE19616381C1/de not_active Expired - Fee Related
-
1997
- 1997-04-23 JP JP9537599A patent/JP2000509266A/ja active Pending
- 1997-04-23 EP EP97923750A patent/EP0910665B1/de not_active Expired - Lifetime
- 1997-04-23 ES ES97923750T patent/ES2179339T3/es not_active Expired - Lifetime
- 1997-04-23 DK DK97923750T patent/DK0910665T3/da active
- 1997-04-23 AT AT97923750T patent/ATE219793T1/de not_active IP Right Cessation
- 1997-04-23 US US09/171,854 patent/US20020137029A1/en not_active Abandoned
- 1997-04-23 DE DE59707607T patent/DE59707607D1/de not_active Expired - Fee Related
- 1997-04-23 WO PCT/DE1997/000814 patent/WO1997040185A1/de not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| US20020137029A1 (en) | 2002-09-26 |
| JP2000509266A (ja) | 2000-07-25 |
| DE59707607D1 (de) | 2002-08-01 |
| DE19616381C1 (de) | 1997-10-23 |
| ATE219793T1 (de) | 2002-07-15 |
| DK0910665T3 (da) | 2002-10-07 |
| EP0910665B1 (de) | 2002-06-26 |
| WO1997040185A1 (de) | 1997-10-30 |
| EP0910665A1 (de) | 1999-04-28 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| NZ538852A (en) | Selection and isolation of living cells using mRNA-binding probes | |
| AR002169A1 (es) | Gen quimerico que expresa la hidroxi-fenil-piruvato-dioxigenasa (hppd), vector, celula de planta y metodo de tratamiento selectivo con herbicidas que lo comprenden | |
| AR030961A1 (es) | Secuencias de nucleotidos que intervienen en la fertilidad masculina en plantas y metodo de uso de las mismas | |
| AR027600A1 (es) | Polipeptidos que tienen actividad de alfa-amilasa alcalina y los acidos nucleicos que lo codifican | |
| MX9400837A (es) | Metodos y aparatos para la determinacion de secuencia de adn. | |
| ES2156978T3 (es) | Uso de exonucleasa y/o glicosilasa como suplementos de un anticuerpo antipolimerasa para aumentar la especifidad en la reaccion en cadena de la polimerasa. | |
| MX9206974A (es) | Metodo para la identificacion de microorganismos mediante la utilizacion de amplificacion de adn; directa y arbitraria. | |
| ES2174063T3 (es) | Procedimiento para la amplificacion de secuencias especificas de acidonucleico. | |
| CO5631459A2 (es) | Metodos para la deteccion de desordenes geneticos | |
| MX338434B (es) | Metodo para crioconservar celulas de esperma seleccionadas. | |
| ATE409238T1 (de) | Nachweis der hybridisierung von nukleinsäuren durch fluoreszenzpolarisation | |
| ATE374249T1 (de) | Datenbanken von regulatorischen sequenzen, methoden zu ihrer herstellung und verwendung | |
| UA49843C2 (uk) | Олігонуклеотид для ампліфікації послідовностей нуклеїнових кислот, спосіб ампліфікації цільової послідовності нуклеїнової кислоти, набір для здійснення реакції ампліфікації нуклеїнової кислоти | |
| PT900279E (pt) | Processo melhorado de transformacao de plantas | |
| ES2570828T3 (es) | Método de análisis de metilación | |
| WO2000031306A3 (en) | Multiplex amplification of short tandem repeat loci | |
| DK0973940T3 (da) | En in vitro-fremgangsmåde til konstruktion af et DNA-bibliotek | |
| ES2172353T3 (es) | Amplificacion del adn de una unica celula. | |
| DK1337629T3 (da) | Dipeptidylpeptidaser | |
| WO2006050499A3 (en) | Single step detection assay | |
| AR101779A2 (es) | Secuencias de nucleótidos msca1 que afectan la fertilidad vegetal masculina y método para usar las mismas | |
| DK0942999T3 (da) | Genfælde-konstrukt til identificering og isolering af gener | |
| ATE474936T1 (de) | Schnelle synthese von oligonukleotiden | |
| ES2286212T3 (es) | Secuencias suprimidas especificamente del genoma de mycobacterium tuberculosis, y su uso en el diagnostico y como vacunas. | |
| ES2584383T3 (es) | Promotores preferidos del cámbium/xilema y usos de los mismos |