ES2302542T3 - Procedimiento para unir secuencias de interes. - Google Patents

Procedimiento para unir secuencias de interes. Download PDF

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Publication number
ES2302542T3
ES2302542T3 ES04762853T ES04762853T ES2302542T3 ES 2302542 T3 ES2302542 T3 ES 2302542T3 ES 04762853 T ES04762853 T ES 04762853T ES 04762853 T ES04762853 T ES 04762853T ES 2302542 T3 ES2302542 T3 ES 2302542T3
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Spain
Prior art keywords
extension
variable region
interest
procedure
sequences
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ES04762853T
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Martin B Oleksiewicz
Lars S Nielsen
Peter S Andersen
Margit H Hansen
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Symphogen AS
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Symphogen AS
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P21/00—Drugs for disorders of the muscular or neuromuscular system
    • A61P21/02—Muscle relaxants, e.g. for tetanus or cramps
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/005—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies constructed by phage libraries
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/12—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from bacteria
    • C07K16/1267—Gram-positive bacteria
    • C07K16/1282—Clostridium (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C40B40/08—Libraries containing RNA or DNA which encodes proteins, e.g. gene libraries
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/21—Immunoglobulins specific features characterized by taxonomic origin from primates, e.g. man
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/55—Fab or Fab'

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Public Health (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Animal Behavior & Ethology (AREA)
  • Virology (AREA)
  • Veterinary Medicine (AREA)
  • Physical Education & Sports Medicine (AREA)
  • Oncology (AREA)
  • Communicable Diseases (AREA)
  • Orthopedic Medicine & Surgery (AREA)
  • Neurology (AREA)
  • Pain & Pain Management (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Un procedimiento de unión de una pluralidad de secuencias de nucleótidos no contiguas de interés, comprendiendo dicho procedimiento: a) amplificar, en un procedimiento de amplificación molecular múltiplex, secuencias de nucleótidos de interés usando un molde derivado de una célula única aislada; y b) efectuar la unión de las secuencias de nucleótidos de interés amplificadas en la etapa a), en el que las secuencias de nucleótidos de interés comprenden secuencias que codifican la región variable y la unión genera un par análogo de secuencias que codifican la región variable.
ES04762853T 2003-09-18 2004-09-17 Procedimiento para unir secuencias de interes. Expired - Lifetime ES2302542T3 (es)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US50445503P 2003-09-18 2003-09-18
US50458903P 2003-09-18 2003-09-18
DKPA200301867 2003-12-17
DKPA200400782 2004-05-15

Publications (1)

Publication Number Publication Date
ES2302542T3 true ES2302542T3 (es) 2008-07-16

Family

ID=44209637

Family Applications (2)

Application Number Title Priority Date Filing Date
ES08152066T Expired - Lifetime ES2375529T3 (es) 2003-09-18 2004-09-17 Procedimiento para unir secuencias de interés.
ES04762853T Expired - Lifetime ES2302542T3 (es) 2003-09-18 2004-09-17 Procedimiento para unir secuencias de interes.

Family Applications Before (1)

Application Number Title Priority Date Filing Date
ES08152066T Expired - Lifetime ES2375529T3 (es) 2003-09-18 2004-09-17 Procedimiento para unir secuencias de interés.

Country Status (18)

Country Link
US (2) US7749697B2 (es)
EP (3) EP1516929A3 (es)
JP (1) JP4651619B2 (es)
CN (2) CN102199593A (es)
AT (2) ATE537256T1 (es)
AU (1) AU2004286019B2 (es)
BR (1) BRPI0414282A (es)
CA (1) CA2539576A1 (es)
DE (1) DE602004012299T2 (es)
DK (2) DK1921144T3 (es)
ES (2) ES2375529T3 (es)
MY (1) MY148907A (es)
NZ (1) NZ545936A (es)
PL (2) PL1670912T3 (es)
PT (2) PT1670912E (es)
SI (2) SI1921144T1 (es)
TW (1) TWI333977B (es)
WO (1) WO2005042774A2 (es)

Families Citing this family (125)

* Cited by examiner, † Cited by third party
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