ES2317113T3 - Escision invasiva de acidos nucleicos. - Google Patents

Escision invasiva de acidos nucleicos. Download PDF

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Publication number
ES2317113T3
ES2317113T3 ES05016686T ES05016686T ES2317113T3 ES 2317113 T3 ES2317113 T3 ES 2317113T3 ES 05016686 T ES05016686 T ES 05016686T ES 05016686 T ES05016686 T ES 05016686T ES 2317113 T3 ES2317113 T3 ES 2317113T3
Authority
ES
Spain
Prior art keywords
nucleic acid
specific
present
acid sequences
nucleic acids
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES05016686T
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English (en)
Inventor
Jeff G Hall
Natascha Lyamicheva
Michael W Kaiser
James E Dahlberg
David Michael Olive
Victor I Lyamichev
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Third Wave Technologies Inc
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Third Wave Technologies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/599,491 external-priority patent/US5846717A/en
Priority claimed from US08/756,386 external-priority patent/US5985557A/en
Priority claimed from US08/758,314 external-priority patent/US6090606A/en
Application filed by Third Wave Technologies Inc filed Critical Third Wave Technologies Inc
Application granted granted Critical
Publication of ES2317113T3 publication Critical patent/ES2317113T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C12Q1/683—Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

Un procedimiento para detectar la presencia de una molécula de ácido nucleico diana que comprende: a) formar una estructura de escisión entre un ácido nucleico diana, un primer oligonucleótido y un segundo oligonucleótido, en la que el ácido nucleico diana comprende una primera región, una segunda región y una tercera región, estando dicha primera región secuencia abajo de y contigua a dicha segunda región, y estando dicha segunda región secuencia abajo de y contigua a dicha tercera región, en la que dicho primer oligonucleótido comprende una parte 3'' y una parte 5'', conteniendo dicha parte 3'' de dicho primer oligonucleótido una secuencia de uno o más nucleótidos que es completamente complementaria a dicha tercera región de dicho ácido nucleico diana, y conteniendo dicha parte 5'' de dicho primer oligonucleótido una secuencia de uno o más nucleótidos adyacente a dicha parte 3'' de dicho primer oligonucleótido que es completamente complementaria a dicha segunda región de dicho ácido nucleico diana, en la que dicho segundo oligonucleótido comprende una parte 3'' y una parte 5'', conteniendo dicha parte 5'' una secuencia de uno o más nucleótidos que es completamente complementaria a dicha primera región de dicho ácido nucleico diana, y en la que dicha parte 3'' de dicho segundo oligonucleótido es complementaria a dicha segunda región de dicho ácido nucleico diana o comprende o está constituida por al menos un solo nucleótido que está apareado erróneamente a dicha segunda región de dicho ácido nucleico diana, siendo dicha estructura de escisión formada cuando dicho primer oligonucleótido se aparea a dicha tercera y dicha segunda región de dicho ácido nucleico diana, y en la que dicho segundo oligonucleótido está apareado a dicha primera región de dicho ácido nucleico diana tal que la parte 3'' de dicho segundo oligonucleótido se solapa con la secuencia de nucleótidos de dicha parte 5'' de dicho primer oligonucleótido apareado a dicha segunda región de dicho ácido nucleico diana; b) escindir dicha estructura de escisión con una enzima termoestable que tiene actividad nucleasa 5'' y bien carece de actividad sintética o comprende actividad sintética reducida en comparación con una ADN polimerasa nativa para generar un producto de escisión no diana, y c) detectar la escisión de dicha estructura de escisión para detectar la presencia de la molécula de ácido nucleico diana.
ES05016686T 1996-01-24 1997-01-22 Escision invasiva de acidos nucleicos. Expired - Lifetime ES2317113T3 (es)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US08/599,491 US5846717A (en) 1996-01-24 1996-01-24 Detection of nucleic acid sequences by invader-directed cleavage
US08/682,853 US6001567A (en) 1996-01-24 1996-07-12 Detection of nucleic acid sequences by invader-directed cleavage
US08/756,386 US5985557A (en) 1996-01-24 1996-11-29 Invasive cleavage of nucleic acids
US08/758,314 US6090606A (en) 1996-01-24 1996-12-02 Cleavage agents
US08/759,038 US6090543A (en) 1996-01-24 1996-12-02 Cleavage of nucleic acids
PCT/US1997/001072 WO1997027214A1 (en) 1996-01-24 1997-01-22 Invasive cleavage of nucleic acids

Publications (1)

Publication Number Publication Date
ES2317113T3 true ES2317113T3 (es) 2009-04-16

Family

ID=27541970

Family Applications (1)

Application Number Title Priority Date Filing Date
ES05016686T Expired - Lifetime ES2317113T3 (es) 1996-01-24 1997-01-22 Escision invasiva de acidos nucleicos.

Country Status (8)

Country Link
EP (2) EP1634890B1 (es)
JP (2) JP3665648B2 (es)
AT (1) ATE414793T1 (es)
AU (1) AU731062B2 (es)
CA (1) CA2243353C (es)
DK (1) DK1634890T3 (es)
ES (1) ES2317113T3 (es)
WO (1) WO1997027214A1 (es)

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AU781188B2 (en) * 1996-01-24 2005-05-12 Third Wave Technologies, Inc. Detection of nucleic acids by multiple sequential invasive cleavages
US6780982B2 (en) * 1996-07-12 2004-08-24 Third Wave Technologies, Inc. Charge tags and the separation of nucleic acid molecules
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Also Published As

Publication number Publication date
CA2243353C (en) 2010-03-30
DK1634890T3 (da) 2009-03-09
JP2002515737A (ja) 2002-05-28
JP3665648B2 (ja) 2005-06-29
JP2005160481A (ja) 2005-06-23
AU731062B2 (en) 2001-03-22
AU1836497A (en) 1997-08-20
JP4295715B2 (ja) 2009-07-15
WO1997027214A1 (en) 1997-07-31
EP0904286A1 (en) 1999-03-31
EP1634890A1 (en) 2006-03-15
CA2243353A1 (en) 1997-07-31
EP1634890B1 (en) 2008-11-19
ATE414793T1 (de) 2008-12-15
EP0904286A4 (en) 2004-01-14

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