JPH01144992A - 融合タンパク質の作成方法 - Google Patents

融合タンパク質の作成方法

Info

Publication number
JPH01144992A
JPH01144992A JP62302153A JP30215387A JPH01144992A JP H01144992 A JPH01144992 A JP H01144992A JP 62302153 A JP62302153 A JP 62302153A JP 30215387 A JP30215387 A JP 30215387A JP H01144992 A JPH01144992 A JP H01144992A
Authority
JP
Japan
Prior art keywords
gene
dhfr
coli
protein
dna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP62302153A
Other languages
English (en)
Japanese (ja)
Other versions
JPH0349559B2 (fr
Inventor
Masahiro Iwakura
正寛 巖倉
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
National Institute of Advanced Industrial Science and Technology AIST
Original Assignee
Agency of Industrial Science and Technology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Agency of Industrial Science and Technology filed Critical Agency of Industrial Science and Technology
Priority to JP62302153A priority Critical patent/JPH01144992A/ja
Publication of JPH01144992A publication Critical patent/JPH01144992A/ja
Publication of JPH0349559B2 publication Critical patent/JPH0349559B2/ja
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0026—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5)
    • C12N9/0028—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5) with NAD or NADP as acceptor (1.5.1)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP62302153A 1987-11-30 1987-11-30 融合タンパク質の作成方法 Granted JPH01144992A (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP62302153A JPH01144992A (ja) 1987-11-30 1987-11-30 融合タンパク質の作成方法

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP62302153A JPH01144992A (ja) 1987-11-30 1987-11-30 融合タンパク質の作成方法

Publications (2)

Publication Number Publication Date
JPH01144992A true JPH01144992A (ja) 1989-06-07
JPH0349559B2 JPH0349559B2 (fr) 1991-07-29

Family

ID=17905547

Family Applications (1)

Application Number Title Priority Date Filing Date
JP62302153A Granted JPH01144992A (ja) 1987-11-30 1987-11-30 融合タンパク質の作成方法

Country Status (1)

Country Link
JP (1) JPH01144992A (fr)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000071689A1 (fr) 1999-05-20 2000-11-30 Novozymes A/S Enzymes subtilases des sous groupes i-s1 et i-s2 possedant au moins un residu acide amine supplementaire entre les positions 127 et 128
WO2000071688A1 (fr) 1999-05-20 2000-11-30 Novozymes A/S Enzymes subtilases des sous-groupes i-s1 et i-s2 comprenant au moins un reste d'acide amine additionnel entre les positions 126 et 127
WO2000071691A1 (fr) 1999-05-20 2000-11-30 Novozymes A/S Enzymes de subtilase des sous-groupes i-s1 et i-s2 possedant au moins un residu supplementaire d'acide amine entre les positions 125 et 126
WO2005074647A2 (fr) 2004-01-30 2005-08-18 Novozymes Inc. Polypeptides presentant une activite favorisant l'activite cellulolytique, et polynucleotides codant lesdits polypeptides
EP2228440A1 (fr) 2003-05-02 2010-09-15 Novozymes Inc. Variantes de beta-glucosidases

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5545395A (en) * 1978-08-11 1980-03-31 Univ California Synthesis of nucleophilic protein by microorganism
JPS6269990A (ja) * 1985-09-24 1987-03-31 Agency Of Ind Science & Technol 発現ベクタ−ｐＴＰ６４−１

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5545395A (en) * 1978-08-11 1980-03-31 Univ California Synthesis of nucleophilic protein by microorganism
JPS6269990A (ja) * 1985-09-24 1987-03-31 Agency Of Ind Science & Technol 発現ベクタ−ｐＴＰ６４−１

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000071689A1 (fr) 1999-05-20 2000-11-30 Novozymes A/S Enzymes subtilases des sous groupes i-s1 et i-s2 possedant au moins un residu acide amine supplementaire entre les positions 127 et 128
WO2000071688A1 (fr) 1999-05-20 2000-11-30 Novozymes A/S Enzymes subtilases des sous-groupes i-s1 et i-s2 comprenant au moins un reste d'acide amine additionnel entre les positions 126 et 127
WO2000071691A1 (fr) 1999-05-20 2000-11-30 Novozymes A/S Enzymes de subtilase des sous-groupes i-s1 et i-s2 possedant au moins un residu supplementaire d'acide amine entre les positions 125 et 126
EP2228440A1 (fr) 2003-05-02 2010-09-15 Novozymes Inc. Variantes de beta-glucosidases
WO2005074647A2 (fr) 2004-01-30 2005-08-18 Novozymes Inc. Polypeptides presentant une activite favorisant l'activite cellulolytique, et polynucleotides codant lesdits polypeptides

Also Published As

Publication number Publication date
JPH0349559B2 (fr) 1991-07-29

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