JPH0555110B2 - - Google Patents

Info

Publication number: JPH0555110B2
Authority: JP; Japan
Prior art keywords: yeast; reductase; dna; cytochrome; cdna
Prior art date: 1987-12-04
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired - Lifetime

Application number

JP62325527A

Other languages

English (en)

Japanese (ja)

Other versions

JPH02451A (ja

Inventor

Yoshasu Yabusaki

Hiroko Murakami

Hideo Ookawa

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

National Institute of Advanced Industrial Science and Technology AIST

Original Assignee

Agency of Industrial Science and Technology

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1987-12-04

Filing date

1987-12-24

Publication date

1993-08-16

1987-12-24 Application filed by Agency of Industrial Science and Technology filed Critical Agency of Industrial Science and Technology

1987-12-24 Priority to JP62325527A priority Critical patent/JPH02451A/ja

1990-01-05 Publication of JPH02451A publication Critical patent/JPH02451A/ja

1993-08-16 Publication of JPH0555110B2 publication Critical patent/JPH0555110B2/ja

Status Granted legal-status Critical Current

Links

240000004808 Saccharomyces cerevisiae Species 0.000 claims description 60
108020004414 DNA Proteins 0.000 claims description 52
108090000623 proteins and genes Proteins 0.000 claims description 23
108010045510 NADPH-Ferrihemoprotein Reductase Proteins 0.000 claims description 13
102100023897 NADPH-cytochrome P450 reductase Human genes 0.000 claims description 10
241000588724 Escherichia coli Species 0.000 claims description 8
244000005700 microbiome Species 0.000 claims description 8
ACFIXJIJDZMPPO-NNYOXOHSSA-N NADPH Chemical compound C1=CCC(C(=O)N)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]2[C@H]([C@@H](OP(O)(O)=O)[C@@H](O2)N2C3=NC=NC(N)=C3N=C2)O)O1 ACFIXJIJDZMPPO-NNYOXOHSSA-N 0.000 claims description 6
229930027945 nicotinamide-adenine dinucleotide Natural products 0.000 claims description 6
230000000813 microbial effect Effects 0.000 claims description 3
108020004511 Recombinant DNA Proteins 0.000 claims description 2
125000003275 alpha amino acid group Chemical group 0.000 claims 1
235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 57
108090000854 Oxidoreductases Proteins 0.000 description 46
102000004316 Oxidoreductases Human genes 0.000 description 45
239000002299 complementary DNA Substances 0.000 description 33
238000000034 method Methods 0.000 description 18
210000004027 cell Anatomy 0.000 description 17
241000700159 Rattus Species 0.000 description 15
108010015742 Cytochrome P-450 Enzyme System Proteins 0.000 description 13
102000002004 Cytochrome P-450 Enzyme System Human genes 0.000 description 12
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 12
150000001413 amino acids Chemical group 0.000 description 12
210000004185 liver Anatomy 0.000 description 12
LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 9
108091008146 restriction endonucleases Proteins 0.000 description 9
239000013598 vector Substances 0.000 description 9
QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 8
102000004190 Enzymes Human genes 0.000 description 8
108090000790 Enzymes Proteins 0.000 description 8
241000283973 Oryctolagus cuniculus Species 0.000 description 8
239000012634 fragment Substances 0.000 description 7
108091032973 (ribonucleotides)n+m Proteins 0.000 description 6
238000009396 hybridization Methods 0.000 description 6
239000000523 sample Substances 0.000 description 6
239000011780 sodium chloride Substances 0.000 description 6
239000000243 solution Substances 0.000 description 6
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 6
238000004519 manufacturing process Methods 0.000 description 5
239000002773 nucleotide Substances 0.000 description 5
125000003729 nucleotide group Chemical group 0.000 description 5
238000012216 screening Methods 0.000 description 5
210000005253 yeast cell Anatomy 0.000 description 5
HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 4
101150053185 P450 gene Proteins 0.000 description 4
239000000872 buffer Substances 0.000 description 4
239000013611 chromosomal DNA Substances 0.000 description 4
238000010367 cloning Methods 0.000 description 4
150000001875 compounds Chemical class 0.000 description 4
239000013604 expression vector Substances 0.000 description 4
108020004999 messenger RNA Proteins 0.000 description 4
230000003228 microsomal effect Effects 0.000 description 4
238000007254 oxidation reaction Methods 0.000 description 4
239000013612 plasmid Substances 0.000 description 4
235000018102 proteins Nutrition 0.000 description 4
102000004169 proteins and genes Human genes 0.000 description 4
235000020183 skimmed milk Nutrition 0.000 description 4
238000005406 washing Methods 0.000 description 4
102000053602 DNA Human genes 0.000 description 3
102000016928 DNA-directed DNA polymerase Human genes 0.000 description 3
108010014303 DNA-directed DNA polymerase Proteins 0.000 description 3
102100034343 Integrase Human genes 0.000 description 3
230000010718 Oxidation Activity Effects 0.000 description 3
108091034057 RNA (poly(A)) Proteins 0.000 description 3
HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
125000000539 amino acid group Chemical group 0.000 description 3
230000001580 bacterial effect Effects 0.000 description 3
239000006167 equilibration buffer Substances 0.000 description 3
230000000984 immunochemical effect Effects 0.000 description 3
239000012528 membrane Substances 0.000 description 3
108020004707 nucleic acids Proteins 0.000 description 3
102000039446 nucleic acids Human genes 0.000 description 3
150000007523 nucleic acids Chemical class 0.000 description 3
238000002360 preparation method Methods 0.000 description 3
241000894007 species Species 0.000 description 3
238000013519 translation Methods 0.000 description 3
102000018832 Cytochromes Human genes 0.000 description 2
108010052832 Cytochromes Proteins 0.000 description 2
KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 2
DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 2
TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 2
SUAKHGWARZSWIH-UHFFFAOYSA-N N,N‐diethylformamide Chemical compound CCN(CC)C=O SUAKHGWARZSWIH-UHFFFAOYSA-N 0.000 description 2
239000000020 Nitrocellulose Substances 0.000 description 2
ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 2
108010092799 RNA-directed DNA polymerase Proteins 0.000 description 2
108091007187 Reductases Proteins 0.000 description 2
235000001014 amino acid Nutrition 0.000 description 2
229940024606 amino acid Drugs 0.000 description 2
238000010804 cDNA synthesis Methods 0.000 description 2
229920002678 cellulose Polymers 0.000 description 2
239000001913 cellulose Substances 0.000 description 2
238000005520 cutting process Methods 0.000 description 2
230000029087 digestion Effects 0.000 description 2
230000027721 electron transport chain Effects 0.000 description 2
238000012869 ethanol precipitation Methods 0.000 description 2
FVTCRASFADXXNN-SCRDCRAPSA-N flavin mononucleotide Chemical compound OP(=O)(O)OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O FVTCRASFADXXNN-SCRDCRAPSA-N 0.000 description 2
FVTCRASFADXXNN-UHFFFAOYSA-N flavin mononucleotide Natural products OP(=O)(O)OCC(O)C(O)C(O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O FVTCRASFADXXNN-UHFFFAOYSA-N 0.000 description 2
239000011768 flavin mononucleotide Substances 0.000 description 2
238000010438 heat treatment Methods 0.000 description 2
238000002955 isolation Methods 0.000 description 2
229920001220 nitrocellulos Polymers 0.000 description 2
SCVFZCLFOSHCOH-UHFFFAOYSA-M potassium acetate Chemical compound [K+].CC([O-])=O SCVFZCLFOSHCOH-UHFFFAOYSA-M 0.000 description 2
235000019231 riboflavin-5'-phosphate Nutrition 0.000 description 2
239000008223 sterile water Substances 0.000 description 2
239000000758 substrate Substances 0.000 description 2
230000002194 synthesizing effect Effects 0.000 description 2
238000012546 transfer Methods 0.000 description 2
125000001493 tyrosinyl group Chemical group [H]OC1=C([H])C([H])=C(C([H])=C1[H])C([H])([H])C([H])(N([H])[H])C(*)=O 0.000 description 2
MTCFGRXMJLQNBG-REOHCLBHSA-N (2S)-2-Amino-3-hydroxypropansäure Chemical compound OC[C@H](N)C(O)=O MTCFGRXMJLQNBG-REOHCLBHSA-N 0.000 description 1
102000007469 Actins Human genes 0.000 description 1
108010085238 Actins Proteins 0.000 description 1
229930024421 Adenine Natural products 0.000 description 1
GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 1
102000007698 Alcohol dehydrogenase Human genes 0.000 description 1
108010021809 Alcohol dehydrogenase Proteins 0.000 description 1
102000002260 Alkaline Phosphatase Human genes 0.000 description 1
108020004774 Alkaline Phosphatase Proteins 0.000 description 1
239000004475 Arginine Substances 0.000 description 1
DCXYFEDJOCDNAF-UHFFFAOYSA-N Asparagine Natural products OC(=O)C(N)CC(N)=O DCXYFEDJOCDNAF-UHFFFAOYSA-N 0.000 description 1
241000972773 Aulopiformes Species 0.000 description 1
108091003079 Bovine Serum Albumin Proteins 0.000 description 1
241000283707 Capra Species 0.000 description 1
108091026890 Coding region Proteins 0.000 description 1
108020004705 Codon Proteins 0.000 description 1
108010066072 DNA modification methylase EcoRI Proteins 0.000 description 1
RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Natural products CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 1
241000588722 Escherichia Species 0.000 description 1
229920001917 Ficoll Polymers 0.000 description 1
WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 1
239000004471 Glycine Substances 0.000 description 1
101710203526 Integrase Proteins 0.000 description 1
108091092195 Intron Proteins 0.000 description 1
XUJNEKJLAYXESH-REOHCLBHSA-N L-Cysteine Chemical compound SC[C@H](N)C(O)=O XUJNEKJLAYXESH-REOHCLBHSA-N 0.000 description 1
ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 description 1
QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 description 1
ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 description 1
DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 description 1
CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 description 1
WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 1
ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 description 1
HNDVDQJCIGZPNO-YFKPBYRVSA-N L-histidine Chemical compound OC(=O)[C@@H](N)CC1=CN=CN1 HNDVDQJCIGZPNO-YFKPBYRVSA-N 0.000 description 1
AGPKZVBTJJNPAG-WHFBIAKZSA-N L-isoleucine Chemical compound CC[C@H](C)[C@H](N)C(O)=O AGPKZVBTJJNPAG-WHFBIAKZSA-N 0.000 description 1
ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 1
KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 description 1
FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 1
COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 description 1
AYFVYJQAPQTCCC-GBXIJSLDSA-N L-threonine Chemical compound C[C@@H](O)[C@H](N)C(O)=O AYFVYJQAPQTCCC-GBXIJSLDSA-N 0.000 description 1
QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 1
KZSNJWFQEVHDMF-BYPYZUCNSA-N L-valine Chemical compound CC(C)[C@H](N)C(O)=O KZSNJWFQEVHDMF-BYPYZUCNSA-N 0.000 description 1
ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 description 1
241000234435 Lilium Species 0.000 description 1
KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
239000004472 Lysine Substances 0.000 description 1
241000124008 Mammalia Species 0.000 description 1
102000008109 Mixed Function Oxygenases Human genes 0.000 description 1
108010074633 Mixed Function Oxygenases Proteins 0.000 description 1
108700026244 Open Reading Frames Proteins 0.000 description 1
108091036407 Polyadenylation Proteins 0.000 description 1
229920001213 Polysorbate 20 Polymers 0.000 description 1
ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 description 1
AUNGANRZJHBGPY-SCRDCRAPSA-N Riboflavin Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-SCRDCRAPSA-N 0.000 description 1
MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 description 1
AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 description 1
239000004473 Threonine Substances 0.000 description 1
QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 1
KZSNJWFQEVHDMF-UHFFFAOYSA-N Valine Natural products CC(C)C(N)C(O)=O KZSNJWFQEVHDMF-UHFFFAOYSA-N 0.000 description 1
229960000643 adenine Drugs 0.000 description 1
238000000246 agarose gel electrophoresis Methods 0.000 description 1
238000007605 air drying Methods 0.000 description 1
235000004279 alanine Nutrition 0.000 description 1
210000004102 animal cell Anatomy 0.000 description 1
239000007864 aqueous solution Substances 0.000 description 1
ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 1
235000009582 asparagine Nutrition 0.000 description 1
229960001230 asparagine Drugs 0.000 description 1
235000003704 aspartic acid Nutrition 0.000 description 1
238000003556 assay Methods 0.000 description 1
238000000211 autoradiogram Methods 0.000 description 1
239000011324 bead Substances 0.000 description 1
OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 description 1
229940098773 bovine serum albumin Drugs 0.000 description 1
229940041514 candida albicans extract Drugs 0.000 description 1
238000006243 chemical reaction Methods 0.000 description 1
239000013599 cloning vector Substances 0.000 description 1
239000005515 coenzyme Substances 0.000 description 1
230000000295 complement effect Effects 0.000 description 1
239000013601 cosmid vector Substances 0.000 description 1
238000012258 culturing Methods 0.000 description 1
XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 description 1
235000018417 cysteine Nutrition 0.000 description 1
230000001419 dependent effect Effects 0.000 description 1
-1 diethyl oxide ester Chemical class 0.000 description 1
238000010828 elution Methods 0.000 description 1
210000003527 eukaryotic cell Anatomy 0.000 description 1
238000000605 extraction Methods 0.000 description 1
229940013640 flavin mononucleotide Drugs 0.000 description 1
230000002068 genetic effect Effects 0.000 description 1
239000011521 glass Substances 0.000 description 1
239000008103 glucose Substances 0.000 description 1
235000013922 glutamic acid Nutrition 0.000 description 1
239000004220 glutamic acid Substances 0.000 description 1
ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 1
102000006602 glyceraldehyde-3-phosphate dehydrogenase Human genes 0.000 description 1
108020004445 glyceraldehyde-3-phosphate dehydrogenase Proteins 0.000 description 1
125000003630 glycyl group Chemical group [H]N([H])C([H])([H])C(*)=O 0.000 description 1
HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 1
229920001519 homopolymer Polymers 0.000 description 1
238000000338 in vitro Methods 0.000 description 1
AGPKZVBTJJNPAG-UHFFFAOYSA-N isoleucine Natural products CCC(C)C(N)C(O)=O AGPKZVBTJJNPAG-UHFFFAOYSA-N 0.000 description 1
229960000310 isoleucine Drugs 0.000 description 1
125000001449 isopropyl group Chemical group [H]C([H])([H])C([H])(*)C([H])([H])[H] 0.000 description 1
229910001629 magnesium chloride Inorganic materials 0.000 description 1
239000003550 marker Substances 0.000 description 1
229930182817 methionine Natural products 0.000 description 1
235000013336 milk Nutrition 0.000 description 1
239000008267 milk Substances 0.000 description 1
210000004080 milk Anatomy 0.000 description 1
239000000203 mixture Substances 0.000 description 1
230000035772 mutation Effects 0.000 description 1
230000003472 neutralizing effect Effects 0.000 description 1
JPXMTWWFLBLUCD-UHFFFAOYSA-N nitro blue tetrazolium(2+) Chemical compound COC1=CC(C=2C=C(OC)C(=CC=2)[N+]=2N(N=C(N=2)C=2C=CC=CC=2)C=2C=CC(=CC=2)[N+]([O-])=O)=CC=C1[N+]1=NC(C=2C=CC=CC=2)=NN1C1=CC=C([N+]([O-])=O)C=C1 JPXMTWWFLBLUCD-UHFFFAOYSA-N 0.000 description 1
230000001590 oxidative effect Effects 0.000 description 1
238000004806 packaging method and process Methods 0.000 description 1
COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 description 1
235000008729 phenylalanine Nutrition 0.000 description 1
229920003023 plastic Polymers 0.000 description 1
239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 1
235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 1
239000001267 polyvinylpyrrolidone Substances 0.000 description 1
235000013855 polyvinylpyrrolidone Nutrition 0.000 description 1
229920000036 polyvinylpyrrolidone Polymers 0.000 description 1
235000011056 potassium acetate Nutrition 0.000 description 1
238000012545 processing Methods 0.000 description 1
230000000644 propagated effect Effects 0.000 description 1
238000005215 recombination Methods 0.000 description 1
230000006798 recombination Effects 0.000 description 1
230000027756 respiratory electron transport chain Effects 0.000 description 1
101150116497 sacm1l gene Proteins 0.000 description 1
235000019515 salmon Nutrition 0.000 description 1
210000001550 testis Anatomy 0.000 description 1
238000001291 vacuum drying Methods 0.000 description 1
239000004474 valine Substances 0.000 description 1
239000012138 yeast extract Substances 0.000 description 1

Landscapes

Enzymes And Modification Thereof (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)

JP62325527A 1987-12-04 1987-12-24 酵母ｎａｄｐｈ−チトクロムｐ４５０還元酵素遺伝子 Granted JPH02451A (ja)

Priority Applications (1)

Application Number	Priority Date	Filing Date	Title
JP62325527A JPH02451A (ja)	1987-12-04	1987-12-24	酵母ｎａｄｐｈ−チトクロムｐ４５０還元酵素遺伝子

Applications Claiming Priority (3)

Application Number	Priority Date	Filing Date	Title
JP30588587		1987-12-04
JP62-305885		1987-12-04
JP62325527A JPH02451A (ja)	1987-12-04	1987-12-24	酵母ｎａｄｐｈ−チトクロムｐ４５０還元酵素遺伝子

Publications (2)

Publication Number	Publication Date
JPH02451A JPH02451A (ja)	1990-01-05
JPH0555110B2 true JPH0555110B2 (de)	1993-08-16

Family

ID=17950486

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
JP62325527A Granted JPH02451A (ja)	1987-12-04	1987-12-24	酵母ｎａｄｐｈ−チトクロムｐ４５０還元酵素遺伝子

Country Status (1)

Country	Link
JP (1)	JPH02451A (de)

1987
- 1987-12-24 JP JP62325527A patent/JPH02451A/ja active Granted

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
ARCHIVES OF BIOCHEMISTRY AND BIOPHISICS=1978 *

Also Published As

Publication number	Publication date
JPH02451A (ja)	1990-01-05

Legal Events

Date	Code	Title	Description
2007-12-24	EXPY	Cancellation because of completion of term

Publication	Publication Date	Title
JP3950196B2 (ja)	2007-07-25	アルコールデヒドロゲナーゼ及びキラルヒドロキシ化合物の酵素的生産へのその使用
Fujioka et al.	1998	A new isoform of human myosin phosphatase targeting/regulatory subunit (MYPT2): cDNA cloning, tissue expression, and chromosomal mapping
Isogai et al.	1990	Structure and expression of cDNA for D-amino acid oxidase active against cephalosporin C from Fusarium solani
CN110551700B (zh)	2022-06-21	Adh蛋白家族突变体及其应用
JP3001933B2 (ja)	2000-01-24	熱安定性シトシンデアミナーゼ
Behal et al.	1989	Cloning and nucleotide sequence of the gene for protein X from Saccharomyces cerevisiae.
JPH10155493A (ja)	1998-06-16	アスペルギルス属由来のホスホリパーゼａ１をコードする遺伝子
JPH10229885A (ja)	1998-09-02	新規アルコールアルデヒド脱水素酵素
JP2571119B2 (ja)	1997-01-16	細胞膜結合型アルデヒド脱水素酸素の構造遺伝子、これを含むプラスミド、形質転換した酢酸菌及び酢酸発酵法
JP5224572B2 (ja)	2013-07-03	デキストラン生成酵素遺伝子、デキストラン生成酵素およびその製造方法、デキストランの製造方法
JP5210530B2 (ja)	2013-06-12	ＲｒｈＪ１Ｉ制限・修飾酵素およびその遺伝子
JPH0555110B2 (de)	1993-08-16
WO1998049275A2 (fr)	1998-11-05	INTEINE D'ARCHAEBACTERIE DE L'ESPECE $i(THERMOCOCCUS FUMICOLANS)
US7183077B2 (en)	2007-02-27	Promoters and gene expression method by using the promoters
JPH0253488A (ja)	1990-02-22	ウリカーゼのｄｎａ配列および製法
JP2007312790A (ja)	2007-12-06	菌類からのα−１，４−グルカンリアーゼ、その精製、遺伝子クローニングおよび微生物での発現
JP5230234B2 (ja)	2013-07-10	還元酵素及びその利用
JP3486942B2 (ja)	2004-01-13	耐熱性エステラーゼ
Tripathi et al.	2006	Overexpression, purification and crystallization of lysine ɛ-aminotransferase (Rv3290c) from Mycobacterium tuberculosis H37Rv
JPH0638771A (ja)	1994-02-15	ヒトプロテインジスルフィドイソメラーゼ遺伝子の発現方法および該遺伝子との共発現によるポリペプチドの製造方法
JP2892171B2 (ja)	1999-05-17	ポリペプチド
JP3024154B2 (ja)	2000-03-21	チトクロムＰ４５０ｃ▲下２▼▲下５▼遺伝子
JP4120964B2 (ja)	2008-07-16	高度好熱菌由来セリンアセチルトランスフェラーゼ及びそれをコードする遺伝子、並びにｌ−システインの酵素合成法
Kim et al.	1995	Gene cloning and characterization of thermostable peptidyl prolyl cis-trans isomerase (PPIase) from Bacillus stearothermophilus SIC1
JP2000316584A (ja)	2000-11-21	カタラーゼ遺伝子