LU87153A1 - Methode pour le dosage d'acides nucleiques,combinaison de reactifs et trousse pour doser ces acides - Google Patents

Methode pour le dosage d'acides nucleiques,combinaison de reactifs et trousse pour doser ces acides Download PDF

Info

Publication number: LU87153A1
Authority: LU; Luxembourg
Prior art keywords: nucleic acid; target nucleic; detection; copies; primers
Prior art date: 1987-03-11

Application number

LU87153A

Other languages

English (en)

French (fr)

Inventor

Hans Soederlund

Arja Weckman

Original Assignee

Orion Yhtymae Oy

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1987-03-11

Filing date

1988-03-10

Publication date

1988-08-23

1988-03-10 Application filed by Orion Yhtymae Oy filed Critical Orion Yhtymae Oy

1988-08-23 Publication of LU87153A1 publication Critical patent/LU87153A1/fr

Links

108020004707 nucleic acids Proteins 0.000 title claims description 80
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238000000034 method Methods 0.000 title claims description 70
239000003153 chemical reaction reagent Substances 0.000 title claims description 22
239000002253 acid Substances 0.000 title claims description 7
150000007513 acids Chemical class 0.000 title description 5
239000000523 sample Substances 0.000 claims description 107
238000001514 detection method Methods 0.000 claims description 81
238000009396 hybridization Methods 0.000 claims description 40
238000006243 chemical reaction Methods 0.000 claims description 24
239000003550 marker Substances 0.000 claims description 17
238000006116 polymerization reaction Methods 0.000 claims description 15
230000001419 dependent effect Effects 0.000 claims description 9
239000011159 matrix material Substances 0.000 claims description 6
239000001226 triphosphate Substances 0.000 claims description 5
239000003795 chemical substances by application Substances 0.000 claims description 4
235000011178 triphosphate Nutrition 0.000 claims description 4
-1 nucleoside triphosphates Chemical class 0.000 claims description 2
229920000642 polymer Polymers 0.000 claims description 2
230000001143 conditioned effect Effects 0.000 claims 1
239000002777 nucleoside Substances 0.000 claims 1
239000012985 polymerization agent Substances 0.000 claims 1
108020004414 DNA Proteins 0.000 description 24
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 24
YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 12
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238000002474 experimental method Methods 0.000 description 5
235000019333 sodium laurylsulphate Nutrition 0.000 description 5
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DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
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108091000080 Phosphotransferase Proteins 0.000 description 1
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150000002500 ions Chemical class 0.000 description 1
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238000003199 nucleic acid amplification method Methods 0.000 description 1
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102000020233 phosphotransferase Human genes 0.000 description 1
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230000002285 radioactive effect Effects 0.000 description 1
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229910052708 sodium Inorganic materials 0.000 description 1
239000011734 sodium Substances 0.000 description 1
239000001632 sodium acetate Substances 0.000 description 1
235000017281 sodium acetate Nutrition 0.000 description 1
229910000029 sodium carbonate Inorganic materials 0.000 description 1
230000009870 specific binding Effects 0.000 description 1
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230000029305 taxis Effects 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
- C12Q1/701—Specific hybridization probes
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]

Landscapes

Chemical & Material Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Organic Chemistry (AREA)
Health & Medical Sciences (AREA)
Wood Science & Technology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Zoology (AREA)
Engineering & Computer Science (AREA)
Immunology (AREA)
Biochemistry (AREA)
Genetics & Genomics (AREA)
Molecular Biology (AREA)
Analytical Chemistry (AREA)
Biotechnology (AREA)
Physics & Mathematics (AREA)
Microbiology (AREA)
General Engineering & Computer Science (AREA)
Biophysics (AREA)
General Health & Medical Sciences (AREA)
Bioinformatics & Cheminformatics (AREA)
Chemical Kinetics & Catalysis (AREA)
Virology (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Investigating Or Analysing Biological Materials (AREA)

LU87153A 1987-03-11 1988-03-10 Methode pour le dosage d'acides nucleiques,combinaison de reactifs et trousse pour doser ces acides LU87153A1 (fr)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
US2460487A	1987-03-11	1987-03-11
US2460487		1987-03-11

Publications (1)

Publication Number	Publication Date
LU87153A1 true LU87153A1 (fr)	1988-08-23

Family

ID=21821443

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
LU87153A LU87153A1 (fr)	1987-03-11	1988-03-10	Methode pour le dosage d'acides nucleiques,combinaison de reactifs et trousse pour doser ces acides

Country Status (25)

Country	Link
US (2)	US5476769A (no)
JP (1)	JP3244500B2 (no)
AT (1)	AT395434B (no)
AU (1)	AU622104B2 (no)
BE (1)	BE1003990A5 (no)
CA (1)	CA1338207C (no)
CH (1)	CH677285A5 (no)
DD (1)	DD272664A5 (no)
DE (1)	DE3807994C2 (no)
DK (1)	DK175384B1 (no)
ES (1)	ES2006377A6 (no)
FI (1)	FI94429C (no)
FR (1)	FR2613077A1 (no)
GB (1)	GB2202328B (no)
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1988
- 1988-02-18 AU AU11937/88A patent/AU622104B2/en not_active Expired
- 1988-02-19 IL IL10215488A patent/IL102154A/en not_active IP Right Cessation
- 1988-02-19 IL IL85480A patent/IL85480A0/xx not_active IP Right Cessation
- 1988-03-01 NZ NZ223700A patent/NZ223700A/en unknown
- 1988-03-03 FI FI880994A patent/FI94429C/fi not_active IP Right Cessation
- 1988-03-09 GR GR880100139A patent/GR1000315B/el not_active IP Right Cessation
- 1988-03-09 DD DD88313526A patent/DD272664A5/de unknown
- 1988-03-10 FR FR8803107A patent/FR2613077A1/fr active Granted
- 1988-03-10 JP JP05731088A patent/JP3244500B2/ja not_active Expired - Lifetime
- 1988-03-10 NL NL8800594A patent/NL194922C/nl not_active IP Right Cessation
- 1988-03-10 BE BE8800267A patent/BE1003990A5/fr not_active IP Right Cessation
- 1988-03-10 IE IE69788A patent/IE61664B1/en not_active IP Right Cessation
- 1988-03-10 CH CH902/88A patent/CH677285A5/de not_active IP Right Cessation
- 1988-03-10 PT PT86937A patent/PT86937B/pt not_active IP Right Cessation
- 1988-03-10 DK DK198801295A patent/DK175384B1/da not_active IP Right Cessation
- 1988-03-10 HU HU881159A patent/HU202583B/hu unknown
- 1988-03-10 GB GB8805681A patent/GB2202328B/en not_active Expired - Lifetime
- 1988-03-10 LU LU87153A patent/LU87153A1/fr unknown
- 1988-03-10 IT IT8819722A patent/IT1216048B/it active
- 1988-03-10 AT AT0064388A patent/AT395434B/de not_active IP Right Cessation
- 1988-03-10 NO NO881064A patent/NO172909C/no not_active IP Right Cessation
- 1988-03-10 CA CA000561135A patent/CA1338207C/en not_active Expired - Fee Related
- 1988-03-10 SE SE8800864A patent/SE500262C2/sv not_active IP Right Cessation
- 1988-03-10 DE DE3807994A patent/DE3807994C2/de not_active Expired - Lifetime
- 1988-03-11 ES ES8800740A patent/ES2006377A6/es not_active Expired
1990
- 1990-11-07 US US07/610,470 patent/US5476769A/en not_active Expired - Lifetime
1992
- 1992-06-10 IL IL102154A patent/IL102154A0/xx unknown
1997
- 1997-08-25 US US08/917,404 patent/US5989817A/en not_active Expired - Fee Related

Also Published As

Publication number	Publication date
FI880994A0 (fi)	1988-03-03
PT86937A (pt)	1989-03-30
IE61664B1 (en)	1994-11-16
ES2006377A6 (es)	1989-04-16
PT86937B (pt)	1995-03-01
ATA64388A (de)	1992-05-15
SE8800864D0 (sv)	1988-03-10
HU202583B (en)	1991-03-28
SE500262C2 (sv)	1994-05-24
IT1216048B (it)	1990-02-22
FI880994L (fi)	1988-09-12
NO172909C (no)	1993-09-22
JPS63243875A (ja)	1988-10-11
IL102154A0 (en)	1993-01-14
GB2202328B (en)	1991-07-03
FI94429B (fi)	1995-05-31
BE1003990A5 (fr)	1992-07-28
NL194922C (nl)	2003-07-04
IE880697L (en)	1988-09-11
CA1338207C (en)	1996-04-02
DD272664A5 (de)	1989-10-18
NL194922B (nl)	2003-03-03
FR2613077A1 (fr)	1988-09-30
NO881064L (no)	1988-09-12
NZ223700A (en)	1990-04-26
AU622104B2 (en)	1992-04-02
AU1193788A (en)	1988-09-15
SE8800864L (sv)	1988-09-12
JP3244500B2 (ja)	2002-01-07
US5476769A (en)	1995-12-19
GB2202328A (en)	1988-09-21
DK175384B1 (da)	2004-09-20
IL85480A0 (en)	1988-07-31
GR880100139A (en)	1989-01-31
FI94429C (fi)	1995-09-11
DE3807994C2 (de)	1998-08-27
GR1000315B (el)	1992-06-25
DK129588A (da)	1988-09-12
AT395434B (de)	1992-12-28
NO172909B (no)	1993-06-14
US5989817A (en)	1999-11-23
IT8819722A0 (it)	1988-03-10
DE3807994A1 (de)	1988-09-22
FR2613077B1 (no)	1994-11-10
CH677285A5 (no)	1991-04-30
DK129588D0 (da)	1988-03-10
NL8800594A (nl)	1988-10-03
IL102154A (en)	1994-04-12
NO881064D0 (no)	1988-03-10
HUT50868A (en)	1990-03-28
GB8805681D0 (en)	1988-04-07

Publication	Publication Date	Title
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EP0158758B1 (fr)	1988-09-28	Sonde contenant un acide nucléique modifié et reconnaissable par des anticorps spécifiques et utilisation de cette sonde et de ces anticorps spécifiques pour détecter et caractériser une séquence d' ADN homologue
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EP0486661B1 (fr)	1996-02-07	Procede de detection d'une sequence nucleotidique selon la technique d'hybridation sandwich
CA2126636C (fr)	2001-12-11	Dispositif pour ameliorer la capture de molecules cibles, et procede de capture utilisant ledit dispositif
JPH07502653A (ja)	1995-03-23	溶液相サンドイッチハイブリダイゼーションアッセイに用いるためのｈｂｖ増幅プローブ
EP0744028A1 (fr)	1996-11-27	Surfaces hautement specifiques pour reactions biologiques, procede pour leur preparation et procede pour leur utilisation
JPH08501689A (ja)	1996-02-27	改良型の鎖置換アッセイおよびそれに有用な複合体
JPS58502165A (ja)	1983-12-15	特異抗体により認識し得る修飾核酸プローブを使用して核酸配列の存在を検出する方法
JP3011987B2 (ja)	2000-02-21	固相化プライマーを用いる逆転写酵素の測定法
EP1185713B1 (fr)	2007-05-02	Procédé de recherche d'une résistance aux anti-protéases d'une souche du virus VIH-2 provenant d'un échantillon biologique prélevé chez un patient
EP0763602B1 (fr)	2003-09-10	Détection des Entérobactéries
EP0195009B1 (fr)	1989-09-06	Acides nucleiques marques chimiquement, leur utilisation et necessaire pour sa mise en oeuvre
FR2746413A1 (fr)	1997-09-26	Detection d'une sequence nucleotidique avec amplification de signal
EP0362042B1 (fr)	1993-06-23	Procédé d'analyse d'une séquence spécifique d'ADN ou d'ARN, réactifs et nécessaires pour sa mise en oeuvre
EP0549776B1 (fr)	1998-12-02	Systeme de sondes permettant d'effectuer le typage hla dr, et procede de typage utilisant lesdites sondes
FR2639358A1 (fr)	1990-05-25	Sondes d'hybridation et procede pour la detection de la presence ou de l'absence d'au moins une sequence variante en un seul test
WO1997046700A1 (fr)	1997-12-11	Sondes nucleotidiques et procede pour determiner le typage hla dqb1
JPS61502637A (ja)	1986-11-13	ヒトｔ−細胞白血病ウイルスタイプ３のインシトウ検出
JP2002533724A (ja)	2002-10-08	異なるマーカーの認識のための試験系、その調製および使用
EP0238593A1 (fr)	1987-09-30	Recuperation et dosage d'adn dans un liquide biologique acellulaire
EP0353124B1 (fr)	1996-03-06	Procédé de marquage d'une sonde nucléique et trousse de réactifs pour la mise en oeuvre de ce procédé
Iitiä et al.	1992	Detection of amplified HTLV-I/-II viral sequences using time-resolved fluorometry
EP0534028A1 (fr)	1993-03-31	Procédé de fixation d'une séquence nucléotidique sur un support solide utilisant un anticorps
FR2718461A1 (fr)	1995-10-13	Procédé de détection d'un site de restriction dans une séquence d'ADN.