LV10120B - Method of producing of new plasmides and using thereof in producing of the plasminogen activator - Google Patents

Method of producing of new plasmides and using thereof in producing of the plasminogen activator Download PDF

Info

Publication number
LV10120B
LV10120B LVP-92-204A LV920204A LV10120B LV 10120 B LV10120 B LV 10120B LV 920204 A LV920204 A LV 920204A LV 10120 B LV10120 B LV 10120B
Authority
LV
Latvia
Prior art keywords
rscu
plasmid
coli
pbf
plasmids
Prior art date
Application number
LVP-92-204A
Other languages
English (en)
Latvian (lv)
Other versions
LV10120A (lv
Inventor
Regina E Brigelius-Flohe
Leopold Flohe
Wolfgang Hillen
Gerd J Steffens
Wolfgang Strassburger
Martin R F Wilhelm
Original Assignee
Gr Nenthal Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gr Nenthal Gmbh filed Critical Gr Nenthal Gmbh
Publication of LV10120A publication Critical patent/LV10120A/xx
Publication of LV10120B publication Critical patent/LV10120B/lv

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6462—Plasminogen activators u-Plasminogen activator (3.4.21.73), i.e. urokinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21073—Serine endopeptidases (3.4.21) u-Plasminogen activator (3.4.21.73), i.e. urokinase

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Peptides Or Proteins (AREA)
LVP-92-204A 1989-07-19 1992-11-13 Method of producing of new plasmides and using thereof in producing of the plasminogen activator LV10120B (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE3923866 1989-07-19

Publications (2)

Publication Number Publication Date
LV10120A LV10120A (lv) 1994-05-10
LV10120B true LV10120B (en) 1994-10-20

Family

ID=6385369

Family Applications (1)

Application Number Title Priority Date Filing Date
LVP-92-204A LV10120B (en) 1989-07-19 1992-11-13 Method of producing of new plasmides and using thereof in producing of the plasminogen activator

Country Status (30)

Country Link
US (1) US5637503A (fr)
EP (1) EP0408945B1 (fr)
JP (1) JP3236284B2 (fr)
KR (1) KR0167761B1 (fr)
AT (1) ATE131534T1 (fr)
AU (1) AU631662B2 (fr)
CA (1) CA2020656C (fr)
DD (1) DD298426A5 (fr)
DE (2) DE4020438A1 (fr)
DK (1) DK0408945T3 (fr)
ES (1) ES2083399T3 (fr)
FI (1) FI102386B (fr)
GR (1) GR3019294T3 (fr)
HK (1) HK1005193A1 (fr)
HR (1) HRP920596B1 (fr)
HU (1) HU214243B (fr)
IE (1) IE901849A1 (fr)
IL (1) IL94529A (fr)
LT (1) LT3611B (fr)
LV (1) LV10120B (fr)
MT (1) MTP1063B (fr)
NO (1) NO304952B1 (fr)
NZ (1) NZ234542A (fr)
PL (1) PL166199B1 (fr)
PT (1) PT94776B (fr)
RU (1) RU2073720C1 (fr)
SI (1) SI9011347B (fr)
SK (1) SK280028B6 (fr)
YU (1) YU48371B (fr)
ZA (1) ZA904006B (fr)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2067226A1 (fr) * 1969-11-27 1971-08-20 Cefilac
DE4101736A1 (de) * 1991-01-22 1992-07-23 Gruenenthal Gmbh Neue als plasminogenaktivatoren einsetzbare polypeptide, dafuer codierende plasmide und verfahren zu deren herstellung und deren verwendung
DE4440892A1 (de) * 1994-11-17 1996-05-23 Gruenenthal Gmbh Proteine mit fibrinolytischen und gerinnungshemmenden Eigenschaften
DE4442665A1 (de) * 1994-11-30 1996-06-05 Gruenenthal Gmbh Chimäre Proteine mit fibrinolytischen und thrombinhemmenden Eigenschaften
KR100467034B1 (ko) * 1996-12-27 2006-04-07 주식회사 엘지생활건강 압축성형타입오일케익화장료및그의제조방법
KR100372233B1 (ko) * 1997-03-12 2003-06-11 주식회사 코리아나화장품 미백 파우더, 그의 제조방법 및 그를 함유하는 메이크업 미백 화장료
RU2140453C1 (ru) * 1999-04-06 1999-10-27 Общество с ограниченной ответственностью Научно-производственное предприятие "Техноген" Рекомбинантная плазмидная днк p ua bc22, кодирующая модифицированный активатор плазминогена урокиназного типа, нетранслируемый днк-элемент - искусственная межгенная последовательность мгп14 и штамм бактерий escherichia coli - продуцент модифицированного активатора плазминогена урокиназного типа
KR100341638B1 (ko) * 2000-03-17 2002-06-22 유상옥,송운한 기능성 표면처리 분체의 제조 방법 및 이를 함유하는메이크업 화장료 조성물
JP4124639B2 (ja) 2002-12-17 2008-07-23 株式会社日本触媒 大腸菌を用いたｓ−ヒドロキシニトリルリアーゼの製造方法
US7906276B2 (en) * 2004-06-30 2011-03-15 Kimberly-Clark Worldwide, Inc. Enzymatic detection techniques
UA100692C2 (ru) 2007-05-02 2013-01-25 Мериал Лимитед Днк-плазмиды, имеющие повышенную экспрессию и стабильность
RU2553533C2 (ru) * 2013-10-23 2015-06-20 Общество с ограниченной ответственностью "Международный биотехнологический центр "Генериум" ("МБЦ "Генериум") Способ выделения и очистки рекомбинантной человеческой проурокиназы м5
CN104555732A (zh) * 2015-01-18 2015-04-29 河南省中原起重机械总厂 一种多功能门式起重机

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4558010A (en) * 1980-04-03 1985-12-10 Abbott Laboratories Recombinant deoxyribonucleic acid which codes for plasminogen activator and method of making plasminogen activator protein therefrom
US4370417A (en) * 1980-04-03 1983-01-25 Abbott Laboratories Recombinant deoxyribonucleic acid which codes for plasminogen activator
IL68366A (en) * 1982-04-15 1990-08-31 Genentech Inc Process for expressing a gene encoding a protein comprising the enzymatic portion of human urokinase in a microorganism or cell culture
US4710464A (en) * 1984-09-27 1987-12-01 Eli Lilly And Company Transcription terminators
DE3680838D1 (de) * 1985-01-25 1991-09-19 Sagami Chem Res Stabilisierte menschliche prourokinase.
FR2594845B1 (fr) * 1986-02-21 1989-12-01 Genetica Preparation par voie microbiologique de l'activateur tissulaire humain du plasminogene (t-pa) et conversion de l'enzyme ainsi obtenue en sa forme active
FI100106B (fi) * 1986-12-05 1997-09-30 Novartis Ag Menetelmä plasminogeenin yksisäikeisen yhdistelmäaktivaattorin valmist amiseksi
EP0303028A1 (fr) * 1987-08-10 1989-02-15 GRUPPO LEPETIT S.p.A. Méthode de préparation d'urokinase à chaîne simple
EP0372005A4 (fr) * 1987-10-30 1990-06-26 Oncogen Systemes d'expression destines a la preparation de polypeptides dans des cellules procaryotiques.

Also Published As

Publication number Publication date
CA2020656C (fr) 2001-05-22
FI102386B1 (fi) 1998-11-30
NZ234542A (en) 1991-09-25
ZA904006B (en) 1991-03-27
LV10120A (lv) 1994-05-10
DD298426A5 (de) 1992-02-20
AU631662B2 (en) 1992-12-03
PL166199B1 (pl) 1995-04-28
HUT54211A (en) 1991-01-28
HRP920596A2 (en) 1998-06-30
HU903970D0 (en) 1990-11-28
IL94529A (en) 1997-11-20
JP3236284B2 (ja) 2001-12-10
KR910003105A (ko) 1991-02-26
CA2020656A1 (fr) 1991-01-20
IL94529A0 (en) 1991-03-10
ATE131534T1 (de) 1995-12-15
LTIP776A (en) 1995-01-31
HU214243B (hu) 1998-03-02
NO902720L (no) 1991-01-21
NO304952B1 (no) 1999-03-08
SK306790A3 (en) 1999-07-12
NO902720D0 (no) 1990-06-19
SI9011347B (sl) 1999-02-28
KR0167761B1 (ko) 1999-01-15
PL286087A1 (en) 1991-03-11
EP0408945B1 (fr) 1995-12-13
YU48371B (sh) 1998-07-10
PT94776A (pt) 1991-03-20
SI9011347A (sl) 1998-04-30
HRP920596B1 (en) 1999-12-31
YU134790A (sh) 1992-12-21
EP0408945A1 (fr) 1991-01-23
DE4020438A1 (de) 1991-01-31
PT94776B (pt) 1997-09-30
JPH0365189A (ja) 1991-03-20
FI903640A0 (fi) 1990-07-18
HK1005193A1 (en) 1998-12-24
FI102386B (fi) 1998-11-30
DK0408945T3 (da) 1996-04-09
MTP1063B (en) 1991-04-08
LT3611B (en) 1995-12-27
RU2073720C1 (ru) 1997-02-20
US5637503A (en) 1997-06-10
IE901849A1 (en) 1991-06-19
GR3019294T3 (en) 1996-06-30
SK280028B6 (sk) 1999-07-12
DE59009961D1 (de) 1996-01-25
ES2083399T3 (es) 1996-04-16
AU5883290A (en) 1991-01-24

Similar Documents

Publication Publication Date Title
Komano et al. The transfer region of IncI1 plasmid R64: similarities between R64 tra and Legionella icm/dot genes
Xie et al. Cloning-independent and counterselectable markerless mutagenesis system in Streptococcus mutans
JP3654648B2 (ja) クローニング及び／または配列ベクター
Actis et al. Bacterial plasmids: replication of extrachromosomal genetic elements encoding resistance to antimicrobial compounds
Günter et al. In vivo evidence for FhuA outer membrane receptor interaction with the TonB inner membrane protein of Escherichia coli
Stibitz et al. The construction of a cloning vector designed for gene replacement in Bordetella pertussis
Matsubara Replication control system in lambda dv
King et al. Transcripts that increase the processivity and elongation rate of RNA polymerase
KR20210016330A (ko) 생산성이 향상된 바이러스 및 비-바이러스 나노 플라스미드 벡터
KR20010071226A (ko) 이형이중나선 돌연변이성 벡터 및 박테리아에 이를이용하는 방법
Dennis et al. Improved antibiotic-resistance cassettes through restriction site elimination using Pfu DNA polymerase PCR
HU197940B (en) Process for producing protein products by means of transformed bacteria
Miles et al. Investigation of the mechanism of active site coupling in the pyruvate dehydrogenase multienzyme complex of Escherichia coli by protein engineering
Chiang et al. Stability of pBR322-derived plasmids
Honda et al. Functional division and reconstruction of a plasmid replication origin: molecular dissection of the oriV of the broad-host-range plasmid RSF1010.
Muller et al. Complete nucleotide sequences of Bacillus plasmids pUB110dB, pRBH1 and its copy mutants
TW201111504A (en) Vector comprising mannose promoter and mannose promoter
Kreiss et al. Production of a new DNA vehicle for gene transfer using site-specific recombination
SI9011347B (sl) Plazmidi, njihova priprava in njihova uporaba pri pridobivanju plazminogenskega aktivatorja
US8877502B2 (en) Plasmid curing
JPH07102136B2 (ja) 組換えｄｎａ、その製法、真核性又は原核性代謝産物及び遺伝子工学的に製造した蛋白質の獲得法
Leemans et al. A broad-host-range expression vector based on the pL promoter of coliphage lambda: regulated synthesis of human interleukin 2 in Erwinia and Serratia species
Bailone et al. Mini-F plasmid-induced SOS signal in Escherichia coli is RecBC dependent.
CY1110458T1 (el) Παραγωγα βενγαμιδης και η χρηση τους στην αγωγη καρκινικων παθησεων
Kozlowski et al. Isolation and structure of the replicon of the promiscuous plasmid pCU1