NO325568B1 - Subtilisinvarianter, DNA, ekspresjonsvektor samt vertscelle. - Google Patents

Subtilisinvarianter, DNA, ekspresjonsvektor samt vertscelle. Download PDF

Info

Publication number
NO325568B1
NO325568B1 NO19961468A NO961468A NO325568B1 NO 325568 B1 NO325568 B1 NO 325568B1 NO 19961468 A NO19961468 A NO 19961468A NO 961468 A NO961468 A NO 961468A NO 325568 B1 NO325568 B1 NO 325568B1
Authority
NO
Norway
Prior art keywords
subtilisin
dna
amino acid
variants
sequence
Prior art date
Application number
NO19961468A
Other languages
English (en)
Norwegian (no)
Other versions
NO961468L (no
NO961468D0 (no
Inventor
Thomas Paul Graycar
Richard R Bott
Lori J Wilson
Original Assignee
Genencor Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genencor Int filed Critical Genencor Int
Publication of NO961468L publication Critical patent/NO961468L/no
Publication of NO961468D0 publication Critical patent/NO961468D0/no
Publication of NO325568B1 publication Critical patent/NO325568B1/no

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38609—Protease or amylase in solid compositions only
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Detergent Compositions (AREA)
  • Peptides Or Proteins (AREA)
  • Saccharide Compounds (AREA)
  • Fats And Perfumes (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Separation Of Suspended Particles By Flocculating Agents (AREA)
NO19961468A 1993-10-14 1996-04-12 Subtilisinvarianter, DNA, ekspresjonsvektor samt vertscelle. NO325568B1 (no)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US13724093A 1993-10-14 1993-10-14
PCT/US1994/011562 WO1995010615A1 (en) 1993-10-14 1994-10-13 Subtilisin variants

Publications (3)

Publication Number Publication Date
NO961468L NO961468L (no) 1996-04-12
NO961468D0 NO961468D0 (no) 1996-04-12
NO325568B1 true NO325568B1 (no) 2008-06-23

Family

ID=22476438

Family Applications (1)

Application Number Title Priority Date Filing Date
NO19961468A NO325568B1 (no) 1993-10-14 1996-04-12 Subtilisinvarianter, DNA, ekspresjonsvektor samt vertscelle.

Country Status (23)

Country Link
US (2) US6586221B2 (pl)
EP (2) EP0723590B1 (pl)
JP (1) JP4086207B2 (pl)
KR (1) KR100344976B1 (pl)
CN (1) CN1056649C (pl)
AT (1) ATE284962T1 (pl)
AU (1) AU700373B2 (pl)
BR (1) BR9407825A (pl)
CA (1) CA2173973C (pl)
CZ (1) CZ289323B6 (pl)
DE (1) DE69434187T2 (pl)
DK (1) DK0723590T3 (pl)
ES (1) ES2235164T3 (pl)
FI (1) FI119697B (pl)
MA (1) MA23346A1 (pl)
NO (1) NO325568B1 (pl)
NZ (1) NZ274998A (pl)
PL (1) PL178478B1 (pl)
PT (1) PT723590E (pl)
RU (1) RU2136756C1 (pl)
TW (1) TW448230B (pl)
WO (1) WO1995010615A1 (pl)
ZA (1) ZA948086B (pl)

Families Citing this family (90)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MA23346A1 (fr) * 1993-10-14 1995-04-01 Genencor Int Variantes de la subtilisine
DE69434962T2 (de) * 1993-10-14 2008-01-17 The Procter & Gamble Company, Cincinnati Proteasehaltige reinigungsmittel
ES2199978T3 (es) * 1993-10-14 2004-03-01 THE PROCTER & GAMBLE COMPANY Composiciones de blanqueo que comprenden enzimas proteasa.
EP0687733A1 (en) * 1994-06-16 1995-12-20 The Procter & Gamble Company Detergent composition containing wool compatible high alkaline proteases
US6066611A (en) * 1994-10-13 2000-05-23 The Procter & Gamble Company Bleaching compositions comprising protease enzymes
US5837517A (en) * 1995-05-05 1998-11-17 Novo Nordisk A/S Protease variants and compositions
SK284609B6 (sk) * 1995-05-05 2005-07-01 Unilever Nv Detergentná zmes obsahujúca subtilázový BLS309 variant
DE19530816A1 (de) * 1995-08-23 1997-02-27 Cognis Bio Umwelt Verwendung von mutierter Subtilisin-Protease in kosmetischen Produkten
DE19535082A1 (de) 1995-09-21 1997-03-27 Henkel Ecolab Gmbh & Co Ohg Pastenförmiges Wasch- und Reinigungsmittel
US5858948A (en) * 1996-05-03 1999-01-12 Procter & Gamble Company Liquid laundry detergent compositions comprising cotton soil release polymers and protease enzymes
DE19636035A1 (de) 1996-09-05 1998-03-12 Henkel Ecolab Gmbh & Co Ohg Pastenförmiges Wasch- und Reinigungsmittel
CN1530443A (zh) * 1996-11-04 2004-09-22 ŵ����÷�����޹�˾ 枯草杆菌酶变异体和组合物
DE19703364A1 (de) 1997-01-30 1998-08-06 Henkel Ecolab Gmbh & Co Ohg Pastenförmiges Wasch- und Reinigungsmittel
BR9810083A (pt) * 1997-06-04 2000-08-08 Procter & Gamble Partìculas de enzimas detergentes tendo uma camada de barreira de carboxilato solúvel em àgua e composições incluindo as mesmas
WO1998055634A1 (en) * 1997-06-04 1998-12-10 The Procter & Gamble Company Protease enzymes for tough cleaning and/or spot and film reduction and compositions incorporating same
BR9810081A (pt) * 1997-06-04 2000-08-08 Procter & Gamble Sistemas de enzima protease mista para limpeza de sujeiras à base de proteìna e composições incorporando os mesmos
US6284246B1 (en) 1997-07-30 2001-09-04 The Procter & Gamble Co. Modified polypeptides with high activity and reduced allergenicity
AU2002325461B2 (en) * 1997-10-23 2006-05-11 Genencor International, Inc. Multiply-substituted protease variants with altered net charge for use in detergents
ES2367505T3 (es) * 1997-10-23 2011-11-04 Danisco Us Inc. Variantes de proteasa con sustituciones múltiples con carga neta alterada para usar en detergentes.
AR016969A1 (es) * 1997-10-23 2001-08-01 Procter & Gamble VARIANTE DE PROTEASA, ADN, VECTOR DE EXPRESIoN, MICROORGANISMO HUESPED, COMPOSICIoN DE LIMPIEZA, ALIMENTO PARA ANIMALES Y COMPOSICIoN PARA TRATAR UN TEXTIL
JP2002500019A (ja) * 1997-12-24 2002-01-08 ジェネンコア インターナショナル インコーポレーテッド 好ましい酵素および/または好ましい洗剤組成物についての改良された分析方法
GB9727470D0 (en) 1997-12-30 1998-02-25 Genencor Int Bv Proteases from gram positive organisms
US6599731B1 (en) 1997-12-30 2003-07-29 Genencor International, Inc. Proteases from gram positive organisms
US6528255B1 (en) 1997-12-30 2003-03-04 Genencor International, Inc. Proteases from gram positive organisms
US6465186B1 (en) 1997-12-30 2002-10-15 Genecor International, Inc. Proteases from gram positive organisms
US6908757B1 (en) 1998-03-26 2005-06-21 The Procter & Gamble Company Serine protease variants having amino acid deletions and substitutions
US6495136B1 (en) 1998-03-26 2002-12-17 The Procter & Gamble Company Proteases having modified amino acid sequences conjugated to addition moieties
BR9909130A (pt) 1998-03-26 2000-12-19 Procter & Gamble Variantes de protease de serina tendo substituições de amino-ácido
DE19857687A1 (de) 1998-12-15 2000-06-21 Henkel Ecolab Gmbh & Co Ohg Pastenförmiges Waschmittel
ES2281976T3 (es) * 1998-12-18 2007-10-01 Novozymes A/S Enzimas subtilasas de los subgrupos i-s1 y i-s2 que tienen un residuo aminoacido adicional en la region del bucle del sitio activo.
DE69941223D1 (de) 1998-12-21 2009-09-17 Billig Fritz Chemisch modifizierte enzymen mit mehrfachgeladenen varianten
CA2376045A1 (en) 1999-07-22 2001-02-01 The Procter & Gamble Company Subtilisin protease variants having amino acid substitutions in defined epitope regions
US6946128B1 (en) 1999-07-22 2005-09-20 The Procter & Gamble Company Protease conjugates having sterically protected epitope regions
BR0012694A (pt) 1999-07-22 2002-04-09 Procter & Gamble Conjugado de protease, composição de limpeza e composição de tratamento pessoal
KR20020021397A (ko) 1999-07-22 2002-03-20 데이비드 엠 모이어 규정된 에피토프 영역에 아미노산 결실 및 치환을 갖는서브틸리신 프로테아제 변이체
CA2382870A1 (en) * 1999-09-09 2001-03-15 Ryohei Ohtani A detergent composition containing a protease
JP2003516751A (ja) * 1999-12-15 2003-05-20 ノボザイムス アクティーゼルスカブ 卵の染みに及ぼす洗浄性能改良を示すズブチラーゼ変異体
CA2394971C (en) * 1999-12-15 2016-01-19 Novozymes A/S Subtilase variants having an improved wash performance on egg stains
US6777218B1 (en) * 2000-03-14 2004-08-17 Novozymes A/S Subtilase enzymes having an improved wash performance on egg stains
US7303907B2 (en) 2001-01-08 2007-12-04 Health Protection Agency Degradation and detection of TSE infectivity
PL210859B1 (pl) * 2001-03-23 2012-03-30 Genencor Int Odmiana subtylizyny zawierająca zmieniony epitop limfocytów T, kwas nukleinowy kodujący tę odmianę subtylizyny, wektor ekspresyjny i komórka gospodarza zawierająca kwas nukleinowy oraz kompozycje zawierające tę odmianę subtylizyny
DK200101090A (da) 2001-07-12 2001-08-16 Novozymes As Subtilase variants
US7153820B2 (en) 2001-08-13 2006-12-26 Ecolab Inc. Solid detergent composition and method for solidifying a detergent composition
US20030157645A1 (en) * 2001-12-21 2003-08-21 Direvo Bio Tech Ag. Subtilisin variants with improved characteristics
BR0215383A (pt) * 2001-12-31 2006-11-28 Genencor Int proteases que produzem resposta imunogênica alterada e métodos de fabricação e uso das mesmas
DK1523553T3 (da) * 2002-01-16 2010-04-19 Genencor Int Multi-substituerede proteasevarianter
WO2003062380A2 (en) * 2002-01-16 2003-07-31 Genencor International, Inc. Multiply-substituted protease variants
CN102766545B (zh) * 2003-05-07 2014-08-06 诺维信公司 变体枯草杆菌蛋白酶(枯草杆菌酶)
US20090060933A1 (en) * 2004-06-14 2009-03-05 Estell David A Proteases producing an altered immunogenic response and methods of making and using the same
EP3061461A1 (en) 2004-10-29 2016-08-31 ratiopharm GmbH Remodeling and glycopegylation of fibroblast growth factor (fgf)
WO2008010925A2 (en) * 2006-07-18 2008-01-24 Danisco Us, Inc., Genencor Division Protease variants active over a broad temperature range
WO2008048537A1 (en) * 2006-10-16 2008-04-24 Danisco Us, Inc. Genencor Division Non-phosphate dish detergents
US8093200B2 (en) 2007-02-15 2012-01-10 Ecolab Usa Inc. Fast dissolving solid detergent
AU2008313267B2 (en) 2007-10-18 2013-09-19 Ecolab Inc. Pressed, waxy, solid cleaning compositions and methods of making them
WO2009149144A2 (en) * 2008-06-06 2009-12-10 Danisco Us Inc. Compositions and methods comprising variant microbial proteases
WO2010056635A1 (en) * 2008-11-11 2010-05-20 Danisco Us Inc. Compositions and methods comprising a subtilisin variant
EP2362896A2 (en) * 2008-11-11 2011-09-07 Danisco US Inc. Bacillus subtilisin comprising one or more combinable mutations
DK3061817T3 (en) * 2009-04-30 2019-03-04 Kao Corp Alkaline protease variants
DE102009029513A1 (de) * 2009-09-16 2011-03-24 Henkel Ag & Co. Kgaa Lagerstabiles flüssiges Wasch- oder Reinigungsmittel enthaltend Proteasen
PL3575389T3 (pl) * 2010-05-06 2025-06-02 The Procter & Gamble Company Produkty konsumenckie z odmianami proteaz
DE102010028951A1 (de) 2010-05-12 2011-11-17 Henkel Ag & Co. Kgaa Lagerstabiles flüssiges Wasch- oder Reinigungsmittel enthaltend Protease und Lipase
DE102010063457A1 (de) * 2010-12-17 2012-06-21 Henkel Ag & Co. Kgaa Lagerstabiles flüssiges Wasch- oder Reinigungsmittel enthaltend Protease und Cellulase
DE102010063458A1 (de) * 2010-12-17 2012-06-21 Henkel Ag & Co. Kgaa Lagerstabiles flüssiges Wasch- oder Reinigungsmittel enthaltend Protease und Amylase
MX378782B (es) * 2011-05-05 2025-03-10 Danisco Us Inc Composiciones y metodos que comprenden variantes de proteasa serina.
US8758520B2 (en) 2011-05-20 2014-06-24 Ecolab Usa Inc. Acid formulations for use in a system for warewashing
AU2012260575B2 (en) 2011-05-20 2015-09-03 Ecolab Usa Inc. Non-phosphate detergents and non-phosphoric acids in an alternating alkali/acid system for warewashing
JP6014163B2 (ja) 2011-12-13 2016-10-25 エコラボ ユーエスエー インコーポレイティド 濃縮物品洗浄組成物及び方法
DE102012201522A1 (de) * 2012-02-02 2013-08-08 Basf Se Lagerstabiles flüssiges Geschirrspülmittel enthaltend Protease und Amylase
EP2628785B1 (en) * 2012-02-17 2016-05-18 Henkel AG & Co. KGaA Detergent compositions comprising subtilase variants
DE102012215107A1 (de) 2012-08-24 2014-02-27 Basf Se Festes Geschirrspülmittel mit verbesserter Proteaseleistung
US9745543B2 (en) 2012-09-10 2017-08-29 Ecolab Usa Inc. Stable liquid manual dishwashing compositions containing enzymes
CN103013960A (zh) * 2012-12-21 2013-04-03 青岛蔚蓝生物集团有限公司 一种碱性蛋白酶及其重组表达工程菌
BR112016005143B1 (pt) 2013-09-09 2021-10-19 Ecolab Usa Inc Composição detergente concentrada, solução aquosa e uso de uma composição detergente concentrada
ES2743376T3 (es) 2013-09-09 2020-02-18 Ecolab Usa Inc Eliminación sinérgica de manchas mediante una nueva combinación de agentes quelantes
CN105683350B (zh) 2013-10-24 2019-03-05 艺康美国股份有限公司 用于从表面上除去污物的组合物和方法
EP3739029A1 (en) 2014-07-04 2020-11-18 Novozymes A/S Subtilase variants and polynucleotides encoding same
RU2710720C2 (ru) * 2014-12-04 2020-01-10 Новозимс А/С Варианты субтилазы и кодирующие их полинуклеотиды
EP3872174B1 (en) * 2015-05-13 2023-03-01 Danisco US Inc. Aprl-clade protease variants and uses thereof
BR112018010475B1 (pt) 2015-11-25 2023-02-14 Unilever Ip Holdings B.V. Composição de detergente líquido e método de lavagem de roupas utilizando uma composição de detergente líquido para lavar roupas
CN109477112B (zh) * 2016-05-31 2024-09-06 丹尼斯科美国公司 蛋白酶变体及其用途
WO2018045200A2 (en) * 2016-09-02 2018-03-08 The Regent Of The University Ofcalifornia Engineered subtiligase variants for versatile, site-specific labeling of proteins
EP3717643A1 (en) * 2017-11-29 2020-10-07 Danisco US Inc. Subtilisin variants having improved stability
CN114207101B (zh) 2019-07-31 2024-05-31 埃科莱布美国股份有限公司 免用个人防护设备的脱灰剂组合物
US11492571B2 (en) 2019-10-24 2022-11-08 The Procter & Gamble Company Automatic dishwashing detergent composition comprising a protease
JP7327661B2 (ja) * 2020-05-15 2023-08-16 Jnc株式会社 抗ウイルス剤
US20250311734A1 (en) 2022-05-14 2025-10-09 Novozymes A/S Compositions and Methods for Preventing, Treating, Suppressing and/or Eliminatting Phytopathogenic Infestations and Infections
CN114836408B (zh) * 2022-05-28 2023-09-19 湖北大学 含有前肽突变体的碱性蛋白酶及应用
EP4446411A1 (en) * 2023-04-14 2024-10-16 Peaccel Variant subtilisin proteases
WO2026030345A1 (en) 2024-07-29 2026-02-05 Danisco Us Inc. Signal and pro-region sequence variants for enhanced protease production in bacillus cells
WO2026093440A1 (en) * 2024-11-04 2026-05-07 Novozymes A/S Protease variants and compositions comprising same

Family Cites Families (37)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4760025A (en) 1984-05-29 1988-07-26 Genencor, Inc. Modified enzymes and methods for making same
US5763257A (en) * 1984-05-29 1998-06-09 Genencor International, Inc. Modified subtilisins having amino acid alterations
US5204015A (en) 1984-05-29 1993-04-20 Genencor International, Inc. Subtilisin mutants
US5155033A (en) 1989-01-06 1992-10-13 Genencor, Inc. Subtilisins modified at position 225 resulting in a shift in catalytic activity
US5182204A (en) 1984-05-29 1993-01-26 Genencor International, Inc. Non-human carbonyl hydrolase mutants, vectors encoding same and hosts transformed with said vectors
US5972682A (en) * 1984-05-29 1999-10-26 Genencor International, Inc. Enzymatically active modified subtilisins
US5185258A (en) 1984-05-29 1993-02-09 Genencor International, Inc. Subtilisin mutants
US4990452A (en) 1986-02-12 1991-02-05 Genex Corporation Combining mutations for stabilization of subtilisin
US5013657A (en) 1988-04-12 1991-05-07 Bryan Philip N Subtilisin mutations
DE3750916T2 (de) * 1986-04-30 1995-06-01 Genencor Int Mutante einer nicht menschlichen Carbonyl-Hydrolase, für diese kodierende DNS-Sequenzen und Vektoren und durch diese Vektoren transformierte Wirte.
WO1988008028A1 (en) * 1987-04-06 1988-10-20 Genex Corporation The engineering of electrostatic interactions at metal ion binding sites for the stabilization of proteins
US4914031A (en) 1987-04-10 1990-04-03 Amgen, Inc. Subtilisin analogs
DK6488D0 (da) * 1988-01-07 1988-01-07 Novo Industri As Enzymer
EP0327801A1 (en) 1988-01-08 1989-08-16 Deutsches Primatenzentrum Gesellschaft Mbh HIV-2-type retroviruses of primates, vaccines, diagnostic and pharmaceutical compositions
US5324653A (en) 1988-02-11 1994-06-28 Gist-Brocades N.V. Recombinant genetic means for the production of serine protease muteins
CN1056187C (zh) 1988-02-11 2000-09-06 金克克国际有限公司 新的蛋白水解酶及其在洗涤剂中的应用
US5116741A (en) * 1988-04-12 1992-05-26 Genex Corporation Biosynthetic uses of thermostable proteases
SE8801426D0 (sv) * 1988-04-15 1988-04-15 Ulf Rothman Forfarande och medel for blodbehandling
KR100188532B1 (ko) * 1989-05-17 1999-06-01 스티븐 엠. 오드리 돌연변이 섭티리신 및 그를 함유하는 조성물
US5665587A (en) * 1989-06-26 1997-09-09 Novo Nordisk A/S Modified subtilisins and detergent compositions containing same
DK316989D0 (da) 1989-06-26 1989-06-26 Novo Nordisk As Enzymer
BR9006832A (pt) * 1989-06-26 1991-08-06 Unilever Nv Composicao detergente enzimatica
ES2227508T3 (es) * 1989-06-26 2005-04-01 Unilever N.V. Composiciones detergentes enzimaticas.
JP2567282B2 (ja) * 1989-10-02 1996-12-25 日本ゼオン株式会社 ポジ型レジスト組成物
DK271490D0 (da) 1990-11-14 1990-11-14 Novo Nordisk As Detergentkomposition
DK0563169T3 (da) 1990-12-21 1998-05-18 Novo Nordisk As Enzymmutanter med lille variation i molekylær ladning i et pH område
US5340735A (en) * 1991-05-29 1994-08-23 Cognis, Inc. Bacillus lentus alkaline protease variants with increased stability
US5316935A (en) * 1992-04-06 1994-05-31 California Institute Of Technology Subtilisin variants suitable for hydrolysis and synthesis in organic media
AU682314B2 (en) 1992-07-17 1997-10-02 Genencor International, Inc. High alkaline serine proteases
DK39093D0 (da) 1993-04-01 1993-04-01 Novo Nordisk As Enzym
MA23346A1 (fr) * 1993-10-14 1995-04-01 Genencor Int Variantes de la subtilisine
DE69434962T2 (de) * 1993-10-14 2008-01-17 The Procter & Gamble Company, Cincinnati Proteasehaltige reinigungsmittel
ES2199978T3 (es) * 1993-10-14 2004-03-01 THE PROCTER & GAMBLE COMPANY Composiciones de blanqueo que comprenden enzimas proteasa.
AR016969A1 (es) * 1997-10-23 2001-08-01 Procter & Gamble VARIANTE DE PROTEASA, ADN, VECTOR DE EXPRESIoN, MICROORGANISMO HUESPED, COMPOSICIoN DE LIMPIEZA, ALIMENTO PARA ANIMALES Y COMPOSICIoN PARA TRATAR UN TEXTIL
US6835550B1 (en) * 1998-04-15 2004-12-28 Genencor International, Inc. Mutant proteins having lower allergenic response in humans and methods for constructing, identifying and producing such proteins
DE69941223D1 (de) * 1998-12-21 2009-09-17 Billig Fritz Chemisch modifizierte enzymen mit mehrfachgeladenen varianten
PL210859B1 (pl) * 2001-03-23 2012-03-30 Genencor Int Odmiana subtylizyny zawierająca zmieniony epitop limfocytów T, kwas nukleinowy kodujący tę odmianę subtylizyny, wektor ekspresyjny i komórka gospodarza zawierająca kwas nukleinowy oraz kompozycje zawierające tę odmianę subtylizyny

Also Published As

Publication number Publication date
ATE284962T1 (de) 2005-01-15
ES2235164T3 (es) 2005-07-01
US20030077807A1 (en) 2003-04-24
RU2136756C1 (ru) 1999-09-10
DK0723590T3 (da) 2005-04-25
DE69434187D1 (de) 2005-01-20
AU700373B2 (en) 1999-01-07
NO961468L (no) 1996-04-12
EP0723590A1 (en) 1996-07-31
EP1526182A2 (en) 2005-04-27
HK1000649A1 (en) 2005-06-03
FI119697B (fi) 2009-02-13
PL178478B1 (pl) 2000-05-31
PT723590E (pt) 2005-04-29
JP4086207B2 (ja) 2008-05-14
TW448230B (en) 2001-08-01
US7250281B2 (en) 2007-07-31
CA2173973C (en) 2009-10-06
PL313942A1 (en) 1996-08-05
KR100344976B1 (ko) 2002-11-23
CN1133068A (zh) 1996-10-09
FI961631L (fi) 1996-04-15
CZ289323B6 (cs) 2002-01-16
WO1995010615A1 (en) 1995-04-20
ZA948086B (en) 1995-06-07
NZ274998A (en) 1997-04-24
EP1526182A3 (en) 2005-05-04
US20040023353A1 (en) 2004-02-05
AU8015794A (en) 1995-05-04
BR9407825A (pt) 1997-05-06
CA2173973A1 (en) 1995-04-20
JPH09504170A (ja) 1997-04-28
US6586221B2 (en) 2003-07-01
DE69434187T2 (de) 2005-12-15
MA23346A1 (fr) 1995-04-01
EP0723590B1 (en) 2004-12-15
FI961631A0 (fi) 1996-04-12
NO961468D0 (no) 1996-04-12
CN1056649C (zh) 2000-09-20
CZ106596A3 (en) 1996-09-11

Similar Documents

Publication Publication Date Title
JP4086207B2 (ja) ズブチリシン変異体
JP3335623B2 (ja) ズブチリシン変異体
US5204015A (en) Subtilisin mutants
JP3776244B2 (ja) プロテアーゼ含有クリーニング組成物
JP5612026B2 (ja) 洗剤で使用される改変された正味の特性を有する複数置換プロテアーゼ変異体
EP0328229B1 (en) Novel proteolytic enzymes and their use in detergents
EP1523553B1 (en) Multiply-substituted protease variants
AU2014336474B2 (en) Protease variants with increased stability
EP2287321B1 (en) Multiply-substituted protease variants
HK1071398A (en) Subtilisin variants
HK1000649B (en) Subtilisin variants
MXPA00003807A (en) Multiply-substituted protease variants
MXPA00003801A (en) Multiply-substituted protease variants
MXPA99004150A (en) Subtilase variants and compositions
MXPA00003806A (en) Multiply-substituted protease variants with altered net charge for use in detergents

Legal Events

Date Code Title Description
MK1K Patent expired