ES2235164T3 - Variantes de subtilisina. - Google Patents

Variantes de subtilisina.

Info

Publication number
ES2235164T3
ES2235164T3 ES94931345T ES94931345T ES2235164T3 ES 2235164 T3 ES2235164 T3 ES 2235164T3 ES 94931345 T ES94931345 T ES 94931345T ES 94931345 T ES94931345 T ES 94931345T ES 2235164 T3 ES2235164 T3 ES 2235164T3
Authority
ES
Spain
Prior art keywords
carbonil
variants
precursory
recombinating
presented
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES94931345T
Other languages
English (en)
Inventor
Thomas P Graycar
Richard R Bott
Lori J Wilson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Danisco US Inc
Original Assignee
Genencor International Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genencor International Inc filed Critical Genencor International Inc
Application granted granted Critical
Publication of ES2235164T3 publication Critical patent/ES2235164T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38609—Protease or amylase in solid compositions only
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Detergent Compositions (AREA)
  • Separation Of Suspended Particles By Flocculating Agents (AREA)
  • Fats And Perfumes (AREA)
  • Saccharide Compounds (AREA)
  • Peptides Or Proteins (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

SE PRESENTAN NUEVAS VARIANTES CARBONIL HIDROLASA DERIVADAS DE SECUENCIAS DE ADN DE CARBONIL HIDROLASA NO HUMANAS NATURALES O RECOMBINANTES . LAS VARIANTES DE LAS CARBONIL HIDROLASAS, EN GENERAL, SE OBTIENEN MEDIANTE UNA MODIFICACION IN VITRO DE UNA SECUENCIA PRECURSORA DE ADN QUE CODIFICA LA CARBONIL HIDROLASA NATURAL O RECOMBINANTE PARA GENERAR LA SUSTITUCION DE UNA PLURALIDAD DE RESIDUOS AMINOACIDOS EN LA SECUENCIA AMINOACIDA DE UNA CARBONIL HIDROLASA PRECURSORA. DICHAS VARIANTES DE LA CARBONIL HIDROLASA TIENEN PROPIEDADES QUE SON DIFERENTES DE AQUELLAS DE LA HIDROLASA PRECURSORA, COMO UNA ACTIVIDAD PROTEOLITICA ALTERADA, UNA ESTABILIDAD ALTERADA ETC. LOS RESIDUOS AMINOACIDOS SUSTITUIDOS CORRESPONDEN A LAS POSICIONES +76 EN COMBINACION CON UNO O MAS DE LOS SIGUIENTES RESIDUOS, +99, +101, +103, +104, +107, +123, +27, +105, +109, +126, +128, +135, +156, +166, +195, +197, +204, +206, +210, +216, +217, +218, +222, +260, +265, Y/O +274 EN LA SUBTILISINA DEL BACILLUS AMYLOLIQUEFACIENS.
ES94931345T 1993-10-14 1994-10-13 Variantes de subtilisina. Expired - Lifetime ES2235164T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US13724093A 1993-10-14 1993-10-14

Publications (1)

Publication Number Publication Date
ES2235164T3 true ES2235164T3 (es) 2005-07-01

Family

ID=22476438

Family Applications (1)

Application Number Title Priority Date Filing Date
ES94931345T Expired - Lifetime ES2235164T3 (es) 1993-10-14 1994-10-13 Variantes de subtilisina.

Country Status (24)

Country Link
US (2) US6586221B2 (es)
EP (2) EP1526182A3 (es)
JP (1) JP4086207B2 (es)
KR (1) KR100344976B1 (es)
CN (1) CN1056649C (es)
AT (1) ATE284962T1 (es)
AU (1) AU700373B2 (es)
BR (1) BR9407825A (es)
CA (1) CA2173973C (es)
CZ (1) CZ289323B6 (es)
DE (1) DE69434187T2 (es)
DK (1) DK0723590T3 (es)
ES (1) ES2235164T3 (es)
FI (1) FI119697B (es)
HK (1) HK1000649A1 (es)
MA (1) MA23346A1 (es)
NO (1) NO325568B1 (es)
NZ (1) NZ274998A (es)
PL (1) PL178478B1 (es)
PT (1) PT723590E (es)
RU (1) RU2136756C1 (es)
TW (1) TW448230B (es)
WO (1) WO1995010615A1 (es)
ZA (1) ZA948086B (es)

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ATE527276T1 (de) * 2001-03-23 2011-10-15 Genencor Int Proteine, die veränderte immunogene reaktionen bewirken, und verfahren zu deren herstellung und anwendung

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WO1995010615A1 (en) 1995-04-20
ATE284962T1 (de) 2005-01-15
NO961468D0 (no) 1996-04-12
NO961468L (no) 1996-04-12
EP0723590A1 (en) 1996-07-31
FI119697B (fi) 2009-02-13
AU8015794A (en) 1995-05-04
US6586221B2 (en) 2003-07-01
PT723590E (pt) 2005-04-29
CZ106596A3 (en) 1996-09-11
RU2136756C1 (ru) 1999-09-10
CN1056649C (zh) 2000-09-20
CA2173973C (en) 2009-10-06
FI961631A0 (fi) 1996-04-12
KR100344976B1 (ko) 2002-11-23
PL178478B1 (pl) 2000-05-31
PL313942A1 (en) 1996-08-05
AU700373B2 (en) 1999-01-07
CZ289323B6 (cs) 2002-01-16
JP4086207B2 (ja) 2008-05-14
EP1526182A2 (en) 2005-04-27
TW448230B (en) 2001-08-01
NO325568B1 (no) 2008-06-23
EP0723590B1 (en) 2004-12-15
CA2173973A1 (en) 1995-04-20
NZ274998A (en) 1997-04-24
FI961631L (fi) 1996-04-15
CN1133068A (zh) 1996-10-09
US7250281B2 (en) 2007-07-31
US20030077807A1 (en) 2003-04-24
US20040023353A1 (en) 2004-02-05
EP1526182A3 (en) 2005-05-04
JPH09504170A (ja) 1997-04-28
BR9407825A (pt) 1997-05-06
DE69434187T2 (de) 2005-12-15
HK1000649A1 (en) 2005-06-03
DK0723590T3 (da) 2005-04-25
ZA948086B (en) 1995-06-07
DE69434187D1 (de) 2005-01-20
MA23346A1 (fr) 1995-04-01

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