PL374311A1 - Sposób oczyszczania protein - Google Patents

Sposób oczyszczania protein

Info

Publication number: PL374311A1
Authority: PL; Poland
Prior art keywords: protein; methods; purifying protein; bind; proteins
Prior art date: 2001-12-21

Application number

PL02374311A

Other languages

English (en)

Inventor

Ganesh Vedantham

Clayton A.Iii Brooks

Joanne M. Reeder

Andrew M. Goetze

Original Assignee

Immunex Corporation

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

2001-12-21

Filing date

2002-12-20

Publication date

2005-10-03

Family has litigation

First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=27407561&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=PL374311(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.

2002-12-20 Application filed by Immunex Corporation filed Critical Immunex Corporation

2005-10-03 Publication of PL374311A1 publication Critical patent/PL374311A1/pl

Links

102000004169 proteins and genes Human genes 0.000 title abstract 11
108090000623 proteins and genes Proteins 0.000 title abstract 11
238000000034 method Methods 0.000 title abstract 3
229910052588 hydroxylapatite Inorganic materials 0.000 abstract 2
XYJRXVWERLGGKC-UHFFFAOYSA-D pentacalcium;hydroxide;triphosphate Chemical compound [OH-].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O XYJRXVWERLGGKC-UHFFFAOYSA-D 0.000 abstract 2
108060003951 Immunoglobulin Proteins 0.000 abstract 1
238000001042 affinity chromatography Methods 0.000 abstract 1
238000004587 chromatography analysis Methods 0.000 abstract 1
102000018358 immunoglobulin Human genes 0.000 abstract 1
239000007787 solid Substances 0.000 abstract 1

Classifications

- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
- C07K14/715—Receptors; Cell surface antigens; Cell surface determinants for cytokines; for lymphokines; for interferons
- C07K14/7151—Receptors; Cell surface antigens; Cell surface determinants for cytokines; for lymphokines; for interferons for tumor necrosis factor [TNF], for lymphotoxin [LT]
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
- C07K14/70575—NGF/TNF-superfamily, e.g. CD70, CD95L, CD153, CD154
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto

Landscapes

Chemical & Material Sciences (AREA)
Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Organic Chemistry (AREA)
General Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Molecular Biology (AREA)
Biochemistry (AREA)
Biophysics (AREA)
Medicinal Chemistry (AREA)
Zoology (AREA)
Immunology (AREA)
Toxicology (AREA)
Gastroenterology & Hepatology (AREA)
Cell Biology (AREA)
Analytical Chemistry (AREA)
Peptides Or Proteins (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)

PL02374311A 2001-12-21 2002-12-20 Sposób oczyszczania protein PL374311A1 (pl)

Applications Claiming Priority (3)

Application Number	Priority Date	Filing Date
US34336301P	2001-12-21	2001-12-21
US34718902P	2002-01-08	2002-01-08
US36427202P	2002-03-12	2002-03-12

Publications (1)

Publication Number	Publication Date
PL374311A1 true PL374311A1 (pl)	2005-10-03

Family

ID=27407561

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
PL02374311A PL374311A1 (pl)	2001-12-21	2002-12-20	Sposób oczyszczania protein

Country Status (11)

Country	Link
US (2)	US7122641B2 (pl)
EP (1)	EP1463942B2 (pl)
JP (1)	JP4363986B2 (pl)
AT (1)	ATE425989T1 (pl)
AU (1)	AU2002359816B2 (pl)
CA (1)	CA2469815C (pl)
DE (1)	DE60231651D1 (pl)
ES (1)	ES2322945T5 (pl)
MX (1)	MXPA04006053A (pl)
PL (1)	PL374311A1 (pl)
WO (1)	WO2003059935A2 (pl)

Families Citing this family (78)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US6406604B1 (en)	1999-11-08	2002-06-18	Norberto A. Guzman	Multi-dimensional electrophoresis apparatus
US7329388B2 (en)	1999-11-08	2008-02-12	Princeton Biochemicals, Inc.	Electrophoresis apparatus having staggered passage configuration
PL374311A1 (pl) *	2001-12-21	2005-10-03	Immunex Corporation	Sposób oczyszczania protein
ES2626268T3 (es)	2002-09-11	2017-07-24	Chugai Seiyaku Kabushiki Kaisha	Método de purificación de proteínas
GB0304576D0 (en) *	2003-02-28	2003-04-02	Lonza Biologics Plc	Protein a chromatography
PL2520654T3 (pl)	2003-08-26	2017-08-31	The Regents Of The University Of Colorado, A Body Corporate	Inhibitory aktywności proteazy serynowej i ich zastosowanie w sposobach i kompozycjach do leczenia zakażeń bakteryjnych
RU2409591C2 (ru)	2003-10-27	2011-01-20	Вайет	Удаление агрегатов с высокой молекулярной массой путем хроматографии на гидроксиапатитах
US8030092B2 (en)	2003-11-07	2011-10-04	Princeton Biochemicals, Inc.	Controlled electrophoresis method
CA2563310A1 (en)	2003-11-07	2005-05-26	Princeton Biochemicals, Inc.	Multi-dimensional electrophoresis apparatus
US20080261249A1 (en) *	2004-01-13	2008-10-23	Genentech, Inc	Detecting and Quantifying Host Cell Proteins in Recombinant Protein Products
JP4872327B2 (ja) *	2004-11-26	2012-02-08	東レ株式会社	改質されたインターロイキン−１１とその製造方法
DK1869065T3 (da)	2005-03-11	2020-06-08	Wyeth Llc	Fremgangsmåde til kromatografi med svag opdeling
US20060240564A1 (en) *	2005-04-20	2006-10-26	Chia-Hui Shieh	pH gradient ion exchange LC-MS and mass compatible buffers
WO2006138181A2 (en)	2005-06-14	2006-12-28	Amgen Inc.	Self-buffering protein formulations
US7375188B2 (en) *	2005-07-29	2008-05-20	Mallinckrodt Baker, Inc.	Vegetarian protein a preparation and methods thereof
MY185597A (en) *	2005-09-20	2021-05-24	Avantor Performance Mat Inc	Vegetarian protein a preparation and methods thereof
EP1816201A1 (en)	2006-02-06	2007-08-08	CSL Behring GmbH	Modified coagulation factor VIIa with extended half-life
EP2738179A1 (en)	2006-04-05	2014-06-04	AbbVie Biotechnology Ltd	Antibody purification
EP1867660A1 (en)	2006-06-14	2007-12-19	CSL Behring GmbH	Proteolytically cleavable fusion protein comprising a blood coagulation factor
JP2010510963A (ja) *	2006-06-14	2010-04-08	グラクソスミスクライン・リミテッド・ライアビリティ・カンパニー	セラミックヒドロキシアパタイトを使用する抗体の精製方法
DK3896090T3 (da)	2006-06-14	2022-03-21	Csl Behring Gmbh	Proteolytisk spalteligt fusionsprotein omfattende en blodkoagutionsfaktor
US7939632B2 (en)	2006-06-14	2011-05-10	Csl Behring Gmbh	Proteolytically cleavable fusion proteins with high molar specific activity
BRPI0716139A2 (pt) *	2006-08-28	2013-09-17	Ares Trading Sa	processo para purificaÇço de proteÍnas de fusço fc
US8513393B2 (en)	2006-08-28	2013-08-20	Ares Trading S.A.	Process for the purification of Fc-containing proteins
JP2010501622A (ja) *	2006-08-28	2010-01-21	アレストレーディングソシエテアノニム	Ｆｃ−融合タンパク質の精製法
US8911964B2 (en)	2006-09-13	2014-12-16	Abbvie Inc.	Fed-batch method of making human anti-TNF-alpha antibody
MX353340B (es)	2006-09-13	2018-01-09	Abbvie Inc	Mejoras de cultivos celulares.
US7488807B2 (en) *	2006-11-22	2009-02-10	3M Innovative Properties Company	Antibody with protein A selectivity
US20100047252A1 (en) *	2006-11-22	2010-02-25	3M Innovative Properties Company	Antibody with protein a selectivity
CA2673459C (en)	2006-12-22	2016-09-13	Stefan Schulte	Modified coagulation factors with prolonged in vivo half-life
US8093364B2 (en)	2008-01-18	2012-01-10	Bio-Rad Laboratories, Inc.	Enhanced purification of antibodies and antibody fragments by apatite chromatography
US20100058173A1 (en) *	2008-08-28	2010-03-04	Kabushiki Kaisha Toshiba	Display processing apparatus, display processing method, and computer program product
EP2334699B1 (en) *	2008-09-23	2013-09-11	F. Hoffmann-La Roche AG	Purification of erythropoietin
SG195577A1 (en)	2008-10-20	2013-12-30	Abbott Lab	Viral inactivation during purification of antibodies
TW201024318A (en) *	2008-10-20	2010-07-01	Abbott Lab	Isolation and purification of antibodies using protein A affinity chromatography
WO2010051360A1 (en) *	2008-10-31	2010-05-06	Wyeth Llc	Purification of acidic proteins using ceramic hydroxyapatite chromatography
US8945895B2 (en) *	2009-07-31	2015-02-03	Baxter International Inc.	Methods of purifying recombinant ADAMTS13 and other proteins and compositions thereof
CN102770458A (zh) *	2009-12-02	2012-11-07	阿塞勒隆制药公司	用于增加Fc融合蛋白的血清半寿期的组合物和方法
JP5773454B2 (ja)	2010-01-15	2015-09-02	バイオ−ラッドラボラトリーズインコーポレーティッド	アパタイトの表面中和方法
NZ603616A (en) *	2010-05-18	2015-02-27	Abbvie Inc	Apparatus and process of purification of proteins
US8895707B2 (en)	2010-08-18	2014-11-25	Bio-Rad Laboratories, Inc.	Elution of proteins from hydroxyapatite resins without resin deterioration
WO2012106449A1 (en) *	2011-02-02	2012-08-09	Bio-Rad Laboratories, Inc.	Apatite surface neutralization with alkali solutions
US9062106B2 (en)	2011-04-27	2015-06-23	Abbvie Inc.	Methods for controlling the galactosylation profile of recombinantly-expressed proteins
US8911992B2 (en) *	2011-04-28	2014-12-16	Bio-Rad Laboratories, Inc.	DNA removal in target molecule purification
EP2718328A4 (en)	2011-06-08	2014-12-24	Acceleron Pharma Inc	COMPOSITIONS AND METHODS FOR INCREASING THE HALF TIME OF SERUM
KR20200044138A (ko)	2011-06-24	2020-04-28	더 리젠츠 오브 더 유니버시티 오브 콜로라도, 어 바디 코포레이트	알파-1 안티트립신 융합 분자용 조성물, 방법 및 용도
JP6280499B2 (ja) *	2011-07-08	2018-02-14	メルク・シャープ・アンド・ドーム・コーポレーションＭｅｒｃｋＳｈａｒｐ＆ＤｏｈｍｅＣｏｒｐ．	Ｆｃ融合タンパク質の精製方法
JP2015504675A (ja)	2012-01-10	2015-02-16	ザリージェンツオブザユニバーシティオブコロラド，アボディーコーポレイトＴＨＥＲＥＧＥＮＴＳＯＦＴＨＥＵＮＩＶＥＲＳＩＴＹＯＦＣＯＬＯＲＡＤＯ，ａｂｏｄｙｃｏｒｐｏｒａｔｅ	アルファ−１アンチトリプシン融合分子の組成物、方法、及び使用
US9067990B2 (en)	2013-03-14	2015-06-30	Abbvie, Inc.	Protein purification using displacement chromatography
WO2013158279A1 (en)	2012-04-20	2013-10-24	Abbvie Inc.	Protein purification methods to reduce acidic species
WO2013158273A1 (en)	2012-04-20	2013-10-24	Abbvie Inc.	Methods to modulate c-terminal lysine variant distribution
US9249182B2 (en)	2012-05-24	2016-02-02	Abbvie, Inc.	Purification of antibodies using hydrophobic interaction chromatography
US9815695B2 (en)	2012-05-30	2017-11-14	Bio-Rad Laboratories, Inc.	In situ restoration of apatite-based chromatography resins
KR20150043523A (ko)	2012-09-02	2015-04-22	애브비 인코포레이티드	단백질 불균일성의 제어 방법
US9512214B2 (en)	2012-09-02	2016-12-06	Abbvie, Inc.	Methods to control protein heterogeneity
EP2895610B1 (en)	2012-09-13	2019-11-06	Indiana University Research and Technology Corporation	Compositions and systems for conferring disease resistance in plants and methods of use thereof
SG11201507230PA (en)	2013-03-12	2015-10-29	Abbvie Inc	Human antibodies that bind human tnf-alpha and methods of preparing the same
US10023608B1 (en)	2013-03-13	2018-07-17	Amgen Inc.	Protein purification methods to remove impurities
US9017687B1 (en)	2013-10-18	2015-04-28	Abbvie, Inc.	Low acidic species compositions and methods for producing and using the same using displacement chromatography
AU2014240596B2 (en)	2013-03-14	2018-09-27	Amgen Inc.	Removal of leaked affinity purification ligand
US8921526B2 (en)	2013-03-14	2014-12-30	Abbvie, Inc.	Mutated anti-TNFα antibodies and methods of their use
WO2014151878A2 (en)	2013-03-14	2014-09-25	Abbvie Inc.	Methods for modulating protein glycosylation profiles of recombinant protein therapeutics using monosaccharides and oligosacharides
EP3052640A2 (en)	2013-10-04	2016-08-10	AbbVie Inc.	Use of metal ions for modulation of protein glycosylation profiles of recombinant proteins
US8946395B1 (en)	2013-10-18	2015-02-03	Abbvie Inc.	Purification of proteins using hydrophobic interaction chromatography
US9085618B2 (en)	2013-10-18	2015-07-21	Abbvie, Inc.	Low acidic species compositions and methods for producing and using the same
US9181337B2 (en)	2013-10-18	2015-11-10	Abbvie, Inc.	Modulated lysine variant species compositions and methods for producing and using the same
WO2015073884A2 (en)	2013-11-15	2015-05-21	Abbvie, Inc.	Glycoengineered binding protein compositions
US9802822B2 (en)	2014-06-23	2017-10-31	Bio-Rad Laboratories, Inc.	Apatite pretreatment
US10099157B2 (en)	2014-06-23	2018-10-16	Bio-Rad Laboratories, Inc.	Apatite in-situ restoration
GB2528301A (en) *	2014-07-17	2016-01-20	Univ Newcastle	Biomolecule separation using apatite
WO2018063980A1 (en) *	2016-09-29	2018-04-05	Bio-Rad Laboratories, Inc.	Agarose-filled ceramic apatite
ES3064802T3 (en)	2016-10-21	2026-04-29	Amgen Inc	Pharmaceutical formulations and methods of making the same
JP7065105B2 (ja) *	2017-02-09	2022-05-11	ブラッコ・スイス・ソシエテ・アノニム	可溶性ｐｓｇｌ－１タンパク質変異体の精製のための方法
US20220009959A1 (en) *	2018-11-26	2022-01-13	North Carolina State University	Peptide ligands for capture of host cell proteins
CN115135392A (zh)	2020-02-19	2022-09-30	生物辐射实验室股份有限公司	制备聚合物填充的色谱树脂的方法
AU2021262609A1 (en) *	2020-05-01	2022-12-22	Kashiv Biosciences, Llc	An improved process of purification of protein
JP2024503239A (ja)	2020-12-22	2024-01-25	アムジェンインコーポレイテッド	細胞培養法
WO2025038763A1 (en) *	2023-08-15	2025-02-20	Bristol-Myers Squibb Company	Ceramic hydroxyapatite chromatography flow through method

Family Cites Families (24)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US4745183A (en) *	1984-02-21	1988-05-17	Bio-Rad Laboratories, Inc.	Use of hydroxylapatite for the analysis and preparation of purified monoclonal antibodies
DE3738541A1 (de)	1987-11-13	1989-05-24	Hoechst Ag	Verfahren zur isolierung und reinigung von hirudin
JPH0774231B2 (ja)	1986-12-11	1995-08-09	東燃株式会社	抗体の分離方法
US4933286A (en)	1987-03-30	1990-06-12	Plant Cell Research Institute, Inc.	Purification of apase-11 and retrieval of the nematode resistance gene
US5115101A (en) *	1988-06-08	1992-05-19	Miles Inc.	Removal of protein A from antibody preparations
JPH022937A (ja)	1988-06-17	1990-01-08	Toray Ind Inc	酵素標識抗体の分離精製方法
JPH03139292A (ja)	1989-05-22	1991-06-13	Toray Ind Inc	ヒトインタ―ロイキン―６に対するモノクロ―ナル抗体
US5395760A (en)	1989-09-05	1995-03-07	Immunex Corporation	DNA encoding tumor necrosis factor-α and -β receptors
DE59010908D1 (de)	1989-09-12	2000-08-10	Hoffmann La Roche	TNF-bindende Proteine
JPH03291300A (ja)	1989-12-28	1991-12-20	Mitsui Toatsu Chem Inc	ポリクローナル抗体の分離方法
GB9007657D0 (en)	1990-04-04	1990-05-30	Connaught Lab	Purification of a pertussis outer membrane protein(omp69)
JPH04368399A (ja)	1991-06-12	1992-12-21	Toagosei Chem Ind Co Ltd	抗体の分離方法
JP2961986B2 (ja)	1991-08-22	1999-10-12	東亞合成株式会社	ポリクローナル抗体の分離精製方法
DE4205938A1 (de) *	1992-02-27	1993-09-02	Medac Klinische Spezialpraep	Immunotoxine
JP3178080B2 (ja)	1992-04-28	2001-06-18	チッソ株式会社	抗体の分離精製方法
US5278284A (en)	1992-05-14	1994-01-11	Miller Brewing Company	Protein purification method
US6099830A (en)	1994-08-09	2000-08-08	Zymogenetics, Inc.	Methods for stimulating erythropoiesis using hematopoietic proteins
US5641655A (en)	1994-11-30	1997-06-24	Zymogenetics, Inc.	Methods for producing thrombopoietin polypeptides using a mammalian tissue plasminogen activator secretory peptide
JPH08188599A (ja)	1995-01-09	1996-07-23	Toagosei Co Ltd	ＩｇＹ抗体の分離精製方法
US5744587A (en)	1995-06-07	1998-04-28	Zymogenetics, Inc.	Method for purifying thrombopoietin
ES2337091T3 (es)	1996-11-27	2010-04-20	Genentech, Inc.	Purificacion por afinidad de polipeptido en una matriz de proteina.
US6121428A (en) *	1997-06-13	2000-09-19	Genentech, Inc.	Protein recovery
PL374311A1 (pl) *	2001-12-21	2005-10-03	Immunex Corporation	Sposób oczyszczania protein
AU2003207496A1 (en) *	2002-01-10	2003-07-30	Syngenta Participations Ag	Phytophthora infestans cdc 14 phosphatase gene, protein and methods of use

2002
- 2002-12-20 PL PL02374311A patent/PL374311A1/pl not_active Application Discontinuation
- 2002-12-20 ES ES02794380T patent/ES2322945T5/es not_active Expired - Lifetime
- 2002-12-20 AU AU2002359816A patent/AU2002359816B2/en not_active Expired
- 2002-12-20 WO PCT/US2002/041159 patent/WO2003059935A2/en not_active Ceased
- 2002-12-20 MX MXPA04006053A patent/MXPA04006053A/es active IP Right Grant
- 2002-12-20 JP JP2003560037A patent/JP4363986B2/ja not_active Expired - Lifetime
- 2002-12-20 US US10/327,495 patent/US7122641B2/en not_active Expired - Lifetime
- 2002-12-20 EP EP02794380A patent/EP1463942B2/en not_active Expired - Lifetime
- 2002-12-20 CA CA2469815A patent/CA2469815C/en not_active Expired - Fee Related
- 2002-12-20 DE DE60231651T patent/DE60231651D1/de not_active Expired - Lifetime
- 2002-12-20 AT AT02794380T patent/ATE425989T1/de not_active IP Right Cessation
2006
- 2006-09-13 US US11/520,921 patent/US7476722B2/en not_active Expired - Lifetime

Also Published As

Publication number	Publication date
ES2322945T5 (es)	2012-10-17
EP1463942B1 (en)	2009-03-18
CA2469815A1 (en)	2003-07-24
MXPA04006053A (es)	2005-03-31
US7476722B2 (en)	2009-01-13
ATE425989T1 (de)	2009-04-15
EP1463942B2 (en)	2012-06-20
WO2003059935A2 (en)	2003-07-24
WO2003059935A3 (en)	2003-10-16
JP2005538686A (ja)	2005-12-22
JP4363986B2 (ja)	2009-11-11
US7122641B2 (en)	2006-10-17
AU2002359816A1 (en)	2003-07-30
DE60231651D1 (de)	2009-04-30
US20030166869A1 (en)	2003-09-04
EP1463942A2 (en)	2004-10-06
CA2469815C (en)	2011-05-03
EP1463942A4 (en)	2005-01-19
US20070010661A1 (en)	2007-01-11
AU2002359816B2 (en)	2006-07-13
ES2322945T3 (es)	2009-07-02

Legal Events

Date	Code	Title	Description
2015-01-30	REFS	Decisions on refusal to grant patents (taken after the publication of the particulars of the applications)

Publication	Publication Date	Title
WO2003059935A3 (en)	2003-10-16	Methods for purifying protein
DK0458841T3 (da)	1995-11-20	Fremgangsmåde til anvendelse og fremstilling af peptider
WO2002083710A3 (en)	2003-05-01	Removal of bacterial endotoxin in a protein solution by immobilized metal affinity chromatography
ATE363491T1 (de)	2007-06-15	Reinigung von antikörpern durch protein a und ionenaustauschromatographie
DE69032806D1 (de)	1999-01-14	Verfahren zur isolierung und reinigung von rezeptoren für transferrin und lactoferrin von bakterien und herstellung von impfstoffen, die sie enthalten
PT1308455E (pt)	2006-08-31	Composicao compreendendo anticorpos anti-her2
ZA951372B (en)	1995-10-24	Antibody purification
KR950700779A (ko)	1995-02-20	단백질의 정제방법(Protein purification)
WO2003104418A3 (en)	2004-09-10	Reconstituted polypeptides
HUP0202532A2 (hu)	2002-11-28	Ko-stimulátor szignál-transzdukciós molekula (AILIM) elleni humán monoklonális antitest és gyógyászati alkalmazása
MX9707909A (es)	1997-11-29	Purificacion de anticuerpos.
ES8506902A1 (es)	1985-08-01	Un procedimiento para la purificacion de un antigeno.
NZ296748A (en)	1999-11-29	Peripheral nervous system specific sodium channel proteins, DNA coding therefor, compositions and methods
ATE361930T1 (de)	2007-06-15	Proteinreinigung mittels chromatographie und darauffolgende filtration auf beladener schicht
DE59912722D1 (de)	2005-12-01	Kontinuierliches verfahren zur trennung von stoffen nach molekülgrösse
ATE419361T1 (de)	2009-01-15	Menschliche monoklonale antikörper spezifisch für antigen e2 des hepatitis c virus
DK1115745T3 (da)	2006-10-02	Oprensning af fibrinogen fra væsker ved udfældning og hydrofob interaktionskromatografi
SE9803224D0 (sv)	1998-09-23	Method for separation of macromolecules
DE50011030D1 (de)	2005-09-29	Verfahren zur chromatographischen fraktionierung von plasma oder serum, so erhaltene präparate und deren verwendung
ATE250966T1 (de)	2003-10-15	Adsoptionschromatographie
DE59508137D1 (de)	2000-05-11	Spezifische bindungssubstanzen für antikörper und deren verwendung für immunoassays oder vakzine
MXPA05011577A (es)	2005-12-15	Purificacion de afinidad a ciclodextrina.
GB9800378D0 (en)	1998-03-04	Proteome analysis
WO1991015582A3 (en)	1991-12-12	Purified rap gaps, rap gap sequences, and uses thereof
ATE246204T1 (de)	2003-08-15	Verfahren zur trennung und/oder isolierung von plasmaproteinen mit annularer chromatographie