SK9372003A3 - Low-lipoxygenase 1 barley - Google Patents

Low-lipoxygenase 1 barley Download PDF

Info

Publication number: SK9372003A3
Authority: SK; Slovakia
Prior art keywords: lox; plant; barley; amino acid; activity
Prior art date: 2000-12-29

Application number

SK937-2003A

Other languages

English (en)

Slovak (sk)

Inventor

Anneke Christiana Douma

Albert Doderer

Varena Cameron-Mills

Birgitte Skadhauge

Lene Molskov Bech

Natalie Schmitt

Jolanda Carolina Heistek

Mechelen Johannes Reinier Van

Original Assignee

Carlsberg Res Lab

Heineken Tech Services

Kronenbourg Brasseries

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

2000-12-29

Filing date

2001-01-22

Publication date

2004-05-04

2000-12-29 Priority claimed from US09/751,687 external-priority patent/US6660915B2/en

2000-12-29 Priority claimed from PCT/IB2000/002045 external-priority patent/WO2002053720A1/en

2001-01-22 Application filed by Carlsberg Res Lab, Heineken Tech Services, Kronenbourg Brasseries filed Critical Carlsberg Res Lab

2004-05-04 Publication of SK9372003A3 publication Critical patent/SK9372003A3/sk

Links

235000007340 Hordeum vulgare Nutrition 0.000 title claims description 116
240000005979 Hordeum vulgare Species 0.000 title description 118
230000000694 effects Effects 0.000 claims abstract description 143
235000013405 beer Nutrition 0.000 claims abstract description 105
GXCLVBGFBYZDAG-UHFFFAOYSA-N N-[2-(1H-indol-3-yl)ethyl]-N-methylprop-2-en-1-amine Chemical compound CN(CCC1=CNC2=C1C=CC=C2)CC=C GXCLVBGFBYZDAG-UHFFFAOYSA-N 0.000 claims abstract description 70
241000196324 Embryophyta Species 0.000 claims abstract description 68
102100025386 Oxidized low-density lipoprotein receptor 1 Human genes 0.000 claims abstract description 39
230000002829 reductive effect Effects 0.000 claims abstract description 32
238000004519 manufacturing process Methods 0.000 claims abstract description 16
235000013361 beverage Nutrition 0.000 claims abstract description 13
241000209219 Hordeum Species 0.000 claims abstract 42
101710199789 Oxidized low-density lipoprotein receptor 1 Proteins 0.000 claims abstract 25
108090000623 proteins and genes Proteins 0.000 claims description 108
235000013339 cereals Nutrition 0.000 claims description 103
BSAIUMLZVGUGKX-BQYQJAHWSA-N (E)-non-2-enal Chemical compound CCCCCC\C=C\C=O BSAIUMLZVGUGKX-BQYQJAHWSA-N 0.000 claims description 96
238000000034 method Methods 0.000 claims description 33
230000035772 mutation Effects 0.000 claims description 31
238000006467 substitution reaction Methods 0.000 claims description 24
230000014509 gene expression Effects 0.000 claims description 22
238000003860 storage Methods 0.000 claims description 22
235000001014 amino acid Nutrition 0.000 claims description 21
125000003275 alpha amino acid group Chemical group 0.000 claims description 17
230000003647 oxidation Effects 0.000 claims description 16
238000007254 oxidation reaction Methods 0.000 claims description 16
125000000539 amino acid group Chemical group 0.000 claims description 14
231100000350 mutagenesis Toxicity 0.000 claims description 12
150000007523 nucleic acids Chemical group 0.000 claims description 12
108091028043 Nucleic acid sequence Proteins 0.000 claims description 11
230000000692 anti-sense effect Effects 0.000 claims description 11
238000002703 mutagenesis Methods 0.000 claims description 11
239000000758 substrate Substances 0.000 claims description 11
CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 claims description 8
150000001413 amino acids Chemical class 0.000 claims description 8
230000002378 acidificating effect Effects 0.000 claims description 7
235000003704 aspartic acid Nutrition 0.000 claims description 7
OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 claims description 7
230000007935 neutral effect Effects 0.000 claims description 7
235000020777 polyunsaturated fatty acids Nutrition 0.000 claims description 7
230000009261 transgenic effect Effects 0.000 claims description 7
230000008569 process Effects 0.000 claims description 6
102000039446 nucleic acids Human genes 0.000 claims description 5
108020004707 nucleic acids Proteins 0.000 claims description 5
235000014113 dietary fatty acids Nutrition 0.000 claims description 4
229930195729 fatty acid Natural products 0.000 claims description 4
239000000194 fatty acid Substances 0.000 claims description 4
230000002209 hydrophobic effect Effects 0.000 claims description 4
238000002741 site-directed mutagenesis Methods 0.000 claims description 4
239000000126 substance Substances 0.000 claims description 4
230000002103 transcriptional effect Effects 0.000 claims description 4
WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 claims description 3
WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 claims description 3
150000004665 fatty acids Chemical class 0.000 claims description 3
235000013922 glutamic acid Nutrition 0.000 claims description 3
239000004220 glutamic acid Substances 0.000 claims description 3
230000005855 radiation Effects 0.000 claims description 3
230000001105 regulatory effect Effects 0.000 claims description 3
FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims 2
235000013311 vegetables Nutrition 0.000 claims 2
108700007698 Genetic Terminator Regions Proteins 0.000 claims 1
229940053200 antiepileptics fatty acid derivative Drugs 0.000 claims 1
230000000087 stabilizing effect Effects 0.000 claims 1
108090000128 Lipoxygenases Proteins 0.000 abstract description 234
102000003820 Lipoxygenases Human genes 0.000 abstract description 232
235000019640 taste Nutrition 0.000 description 50
239000000284 extract Substances 0.000 description 32
238000003556 assay Methods 0.000 description 31
239000000047 product Substances 0.000 description 26
102000004169 proteins and genes Human genes 0.000 description 26
210000002257 embryonic structure Anatomy 0.000 description 24
235000018102 proteins Nutrition 0.000 description 24
108020004414 DNA Proteins 0.000 description 23
102000053602 DNA Human genes 0.000 description 23
102000004190 Enzymes Human genes 0.000 description 21
108090000790 Enzymes Proteins 0.000 description 21
238000003752 polymerase chain reaction Methods 0.000 description 19
DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 18
210000001938 protoplast Anatomy 0.000 description 18
235000010469 Glycine max Nutrition 0.000 description 17
QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 17
230000002068 genetic effect Effects 0.000 description 17
238000002474 experimental method Methods 0.000 description 16
239000012634 fragment Substances 0.000 description 16
108700028369 Alleles Proteins 0.000 description 15
244000068988 Glycine max Species 0.000 description 15
239000013615 primer Substances 0.000 description 15
101150106914 LOX1 gene Proteins 0.000 description 14
101150014436 LOX1.1 gene Proteins 0.000 description 14
101150086211 OLR1 gene Proteins 0.000 description 14
101100455173 Phaseolus vulgaris LOXA gene Proteins 0.000 description 14
101100182222 Solanum tuberosum LOX1.2 gene Proteins 0.000 description 14
230000015572 biosynthetic process Effects 0.000 description 14
238000001514 detection method Methods 0.000 description 14
HCZKYJDFEPMADG-TXEJJXNPSA-N masoprocol Chemical compound C([C@H](C)[C@H](C)CC=1C=C(O)C(O)=CC=1)C1=CC=C(O)C(O)=C1 HCZKYJDFEPMADG-TXEJJXNPSA-N 0.000 description 13
239000002773 nucleotide Substances 0.000 description 13
125000003729 nucleotide group Chemical group 0.000 description 13
210000004027 cell Anatomy 0.000 description 11
239000002299 complementary DNA Substances 0.000 description 11
101100128617 Arabidopsis thaliana LOX3 gene Proteins 0.000 description 10
101100455162 Arabidopsis thaliana LOX4 gene Proteins 0.000 description 10
108091026890 Coding region Proteins 0.000 description 10
101100455161 Glycine max LOX1.3 gene Proteins 0.000 description 10
108010044467 Isoenzymes Proteins 0.000 description 10
OYHQOLUKZRVURQ-HZJYTTRNSA-N Linoleic acid Chemical compound CCCCC\C=C/C\C=C/CCCCCCCC(O)=O OYHQOLUKZRVURQ-HZJYTTRNSA-N 0.000 description 10
PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 description 10
235000020778 linoleic acid Nutrition 0.000 description 10
OYHQOLUKZRVURQ-IXWMQOLASA-N linoleic acid Natural products CCCCC\C=C/C\C=C\CCCCCCCC(O)=O OYHQOLUKZRVURQ-IXWMQOLASA-N 0.000 description 10
239000004471 Glycine Substances 0.000 description 9
230000002255 enzymatic effect Effects 0.000 description 9
JARKCYVAAOWBJS-UHFFFAOYSA-N hexanal Chemical compound CCCCCC=O JARKCYVAAOWBJS-UHFFFAOYSA-N 0.000 description 9
210000001161 mammalian embryo Anatomy 0.000 description 9
238000012216 screening Methods 0.000 description 9
238000006243 chemical reaction Methods 0.000 description 8
235000013305 food Nutrition 0.000 description 8
230000037361 pathway Effects 0.000 description 8
229910021653 sulphate ion Inorganic materials 0.000 description 8
229940024606 amino acid Drugs 0.000 description 7
229940098396 barley grain Drugs 0.000 description 7
230000001488 breeding effect Effects 0.000 description 7
HCZKYJDFEPMADG-UHFFFAOYSA-N erythro-nordihydroguaiaretic acid Natural products C=1C=C(O)C(O)=CC=1CC(C)C(C)CC1=CC=C(O)C(O)=C1 HCZKYJDFEPMADG-UHFFFAOYSA-N 0.000 description 7
230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 7
108700039691 Genetic Promoter Regions Proteins 0.000 description 6
OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 6
239000002202 Polyethylene glycol Substances 0.000 description 6
239000011543 agarose gel Substances 0.000 description 6
108010050181 aleurone Proteins 0.000 description 6
238000013459 approach Methods 0.000 description 6
238000000855 fermentation Methods 0.000 description 6
230000004151 fermentation Effects 0.000 description 6
239000000796 flavoring agent Substances 0.000 description 6
235000019634 flavors Nutrition 0.000 description 6
238000001114 immunoprecipitation Methods 0.000 description 6
229960003951 masoprocol Drugs 0.000 description 6
230000035800 maturation Effects 0.000 description 6
239000013612 plasmid Substances 0.000 description 6
229920001223 polyethylene glycol Polymers 0.000 description 6
230000000644 propagated effect Effects 0.000 description 6
238000007894 restriction fragment length polymorphism technique Methods 0.000 description 6
230000009466 transformation Effects 0.000 description 6
JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 5
239000007995 HEPES buffer Substances 0.000 description 5
238000012408 PCR amplification Methods 0.000 description 5
230000032683 aging Effects 0.000 description 5
KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 description 5
239000004327 boric acid Substances 0.000 description 5
238000009395 breeding Methods 0.000 description 5
239000000872 buffer Substances 0.000 description 5
238000003776 cleavage reaction Methods 0.000 description 5
238000010411 cooking Methods 0.000 description 5
238000011161 development Methods 0.000 description 5
230000018109 developmental process Effects 0.000 description 5
239000003112 inhibitor Substances 0.000 description 5
239000000463 material Substances 0.000 description 5
230000002441 reversible effect Effects 0.000 description 5
230000007017 scission Effects 0.000 description 5
238000012360 testing method Methods 0.000 description 5
FYGDTMLNYKFZSV-URKRLVJHSA-N (2s,3r,4s,5s,6r)-2-[(2r,4r,5r,6s)-4,5-dihydroxy-2-(hydroxymethyl)-6-[(2r,4r,5r,6s)-4,5,6-trihydroxy-2-(hydroxymethyl)oxan-3-yl]oxyoxan-3-yl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1[C@@H](CO)O[C@@H](OC2[C@H](O[C@H](O)[C@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O FYGDTMLNYKFZSV-URKRLVJHSA-N 0.000 description 4
QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 4
IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
229920002498 Beta-glucan Polymers 0.000 description 4
XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 4
240000007594 Oryza sativa Species 0.000 description 4
235000007164 Oryza sativa Nutrition 0.000 description 4
ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 description 4
240000004808 Saccharomyces cerevisiae Species 0.000 description 4
108700019146 Transgenes Proteins 0.000 description 4
240000008042 Zea mays Species 0.000 description 4
235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 description 4
235000002017 Zea mays subsp mays Nutrition 0.000 description 4
238000004458 analytical method Methods 0.000 description 4
125000002915 carbonyl group Chemical group [*:2]C([*:1])=O 0.000 description 4
238000001035 drying Methods 0.000 description 4
238000005516 engineering process Methods 0.000 description 4
239000013604 expression vector Substances 0.000 description 4
230000035784 germination Effects 0.000 description 4
235000009973 maize Nutrition 0.000 description 4
230000001404 mediated effect Effects 0.000 description 4
YBYRMVIVWMBXKQ-UHFFFAOYSA-N phenylmethanesulfonyl fluoride Chemical compound FS(=O)(=O)CC1=CC=CC=C1 YBYRMVIVWMBXKQ-UHFFFAOYSA-N 0.000 description 4
230000000306 recurrent effect Effects 0.000 description 4
108091008146 restriction endonucleases Proteins 0.000 description 4
235000009566 rice Nutrition 0.000 description 4
239000000523 sample Substances 0.000 description 4
239000006228 supernatant Substances 0.000 description 4
238000013518 transcription Methods 0.000 description 4
230000035897 transcription Effects 0.000 description 4
238000012546 transfer Methods 0.000 description 4
230000010474 transient expression Effects 0.000 description 4
238000001262 western blot Methods 0.000 description 4
QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
108091092236 Chimeric RNA Proteins 0.000 description 3
MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 3
ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 description 3
108091034117 Oligonucleotide Proteins 0.000 description 3
UCKMPCXJQFINFW-UHFFFAOYSA-N Sulphide Chemical compound [S-2] UCKMPCXJQFINFW-UHFFFAOYSA-N 0.000 description 3
108090000848 Ubiquitin Proteins 0.000 description 3
102000044159 Ubiquitin Human genes 0.000 description 3
JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 3
239000002253 acid Substances 0.000 description 3
DTOSIQBPPRVQHS-PDBXOOCHSA-N alpha-linolenic acid Chemical compound CC\C=C/C\C=C/C\C=C/CCCCCCCC(O)=O DTOSIQBPPRVQHS-PDBXOOCHSA-N 0.000 description 3
235000020661 alpha-linolenic acid Nutrition 0.000 description 3
238000013124 brewing process Methods 0.000 description 3
238000006555 catalytic reaction Methods 0.000 description 3
230000008859 change Effects 0.000 description 3
150000001875 compounds Chemical class 0.000 description 3
230000029087 digestion Effects 0.000 description 3
238000011156 evaluation Methods 0.000 description 3
239000007789 gas Substances 0.000 description 3
125000001165 hydrophobic group Chemical group 0.000 description 3
238000003780 insertion Methods 0.000 description 3
230000037431 insertion Effects 0.000 description 3
239000002198 insoluble material Substances 0.000 description 3
229960004488 linolenic acid Drugs 0.000 description 3
KQQKGWQCNNTQJW-UHFFFAOYSA-N linolenic acid Natural products CC=CCCC=CCC=CCCCCCCCC(O)=O KQQKGWQCNNTQJW-UHFFFAOYSA-N 0.000 description 3
150000002632 lipids Chemical class 0.000 description 3
238000004890 malting Methods 0.000 description 3
108020004999 messenger RNA Proteins 0.000 description 3
230000029553 photosynthesis Effects 0.000 description 3
238000010672 photosynthesis Methods 0.000 description 3
230000037039 plant physiology Effects 0.000 description 3
238000002360 preparation method Methods 0.000 description 3
108090000765 processed proteins & peptides Proteins 0.000 description 3
238000012545 processing Methods 0.000 description 3
230000000284 resting effect Effects 0.000 description 3
238000005204 segregation Methods 0.000 description 3
238000013519 translation Methods 0.000 description 3
239000013598 vector Substances 0.000 description 3
108091032973 (ribonucleotides)n+m Proteins 0.000 description 2
BFKLIGQXXNIIEL-ODZAUARKSA-N (z)-but-2-enedioic acid;hydrazine Chemical compound NN.OC(=O)\C=C/C(O)=O BFKLIGQXXNIIEL-ODZAUARKSA-N 0.000 description 2
IKHGUXGNUITLKF-UHFFFAOYSA-N Acetaldehyde Chemical compound CC=O IKHGUXGNUITLKF-UHFFFAOYSA-N 0.000 description 2
102000002260 Alkaline Phosphatase Human genes 0.000 description 2
108020004774 Alkaline Phosphatase Proteins 0.000 description 2
QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
241000252095 Congridae Species 0.000 description 2
239000003155 DNA primer Substances 0.000 description 2
QSJXEFYPDANLFS-UHFFFAOYSA-N Diacetyl Chemical group CC(=O)C(C)=O QSJXEFYPDANLFS-UHFFFAOYSA-N 0.000 description 2
PLUBXMRUUVWRLT-UHFFFAOYSA-N Ethyl methanesulfonate Chemical compound CCOS(C)(=O)=O PLUBXMRUUVWRLT-UHFFFAOYSA-N 0.000 description 2
108700024394 Exon Proteins 0.000 description 2
101100128612 Glycine max LOX1.2 gene Proteins 0.000 description 2
102000001554 Hemoglobins Human genes 0.000 description 2
108010054147 Hemoglobins Proteins 0.000 description 2
OAKJQQAXSVQMHS-UHFFFAOYSA-N Hydrazine Chemical compound NN OAKJQQAXSVQMHS-UHFFFAOYSA-N 0.000 description 2
108091092195 Intron Proteins 0.000 description 2
108010025815 Kanamycin Kinase Proteins 0.000 description 2
101150046318 LOX2 gene Proteins 0.000 description 2
101100343701 Mus musculus Loxl1 gene Proteins 0.000 description 2
ZRKWMRDKSOPRRS-UHFFFAOYSA-N N-Methyl-N-nitrosourea Chemical compound O=NN(C)C(N)=O ZRKWMRDKSOPRRS-UHFFFAOYSA-N 0.000 description 2
229930193140 Neomycin Natural products 0.000 description 2
238000000636 Northern blotting Methods 0.000 description 2
108700026244 Open Reading Frames Proteins 0.000 description 2
108700008625 Reporter Genes Proteins 0.000 description 2
YDBYJHTYSHBBAU-YFKPBYRVSA-N S-methyl-L-methioninate Chemical compound C[S+](C)CC[C@H](N)C([O-])=O YDBYJHTYSHBBAU-YFKPBYRVSA-N 0.000 description 2
WYURNTSHIVDZCO-UHFFFAOYSA-N Tetrahydrofuran Chemical compound C1CCOC1 WYURNTSHIVDZCO-UHFFFAOYSA-N 0.000 description 2
235000006085 Vigna mungo var mungo Nutrition 0.000 description 2
240000005616 Vigna mungo var. mungo Species 0.000 description 2
230000009418 agronomic effect Effects 0.000 description 2
150000001299 aldehydes Chemical class 0.000 description 2
230000003321 amplification Effects 0.000 description 2
229940009098 aspartate Drugs 0.000 description 2
239000012131 assay buffer Substances 0.000 description 2
QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
238000009835 boiling Methods 0.000 description 2
-1 carbon chain aldehydes Chemical class 0.000 description 2
150000001728 carbonyl compounds Chemical class 0.000 description 2
238000005119 centrifugation Methods 0.000 description 2
239000002962 chemical mutagen Substances 0.000 description 2
230000000052 comparative effect Effects 0.000 description 2
239000013078 crystal Substances 0.000 description 2
KSMVZQYAVGTKIV-UHFFFAOYSA-N decanal Chemical compound CCCCCCCCCC=O KSMVZQYAVGTKIV-UHFFFAOYSA-N 0.000 description 2
230000003247 decreasing effect Effects 0.000 description 2
230000001066 destructive effect Effects 0.000 description 2
230000009483 enzymatic pathway Effects 0.000 description 2
238000000605 extraction Methods 0.000 description 2
125000003630 glycyl group Chemical group [H]N([H])C([H])([H])C(*)=O 0.000 description 2
239000001963 growth medium Substances 0.000 description 2
238000003306 harvesting Methods 0.000 description 2
238000011534 incubation Methods 0.000 description 2
229910052742 iron Inorganic materials 0.000 description 2
238000002386 leaching Methods 0.000 description 2
108010073854 lipoxygenase 3 Proteins 0.000 description 2
238000005259 measurement Methods 0.000 description 2
230000007246 mechanism Effects 0.000 description 2
230000004060 metabolic process Effects 0.000 description 2
229960005173 methiosulfonium chloride Drugs 0.000 description 2
229960004927 neomycin Drugs 0.000 description 2
229910052757 nitrogen Inorganic materials 0.000 description 2
238000003199 nucleic acid amplification method Methods 0.000 description 2
229910052760 oxygen Inorganic materials 0.000 description 2
239000001301 oxygen Substances 0.000 description 2
238000004806 packaging method and process Methods 0.000 description 2
239000002245 particle Substances 0.000 description 2
230000008635 plant growth Effects 0.000 description 2
229920001184 polypeptide Polymers 0.000 description 2
102000004196 processed proteins & peptides Human genes 0.000 description 2
230000009467 reduction Effects 0.000 description 2
238000004007 reversed phase HPLC Methods 0.000 description 2
238000007423 screening assay Methods 0.000 description 2
230000010153 self-pollination Effects 0.000 description 2
238000002864 sequence alignment Methods 0.000 description 2
238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 2
230000002194 synthesizing effect Effects 0.000 description 2
238000011426 transformation method Methods 0.000 description 2
238000011282 treatment Methods 0.000 description 2
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
MTCFGRXMJLQNBG-REOHCLBHSA-N (2S)-2-Amino-3-hydroxypropansäure Chemical compound OC[C@H](N)C(O)=O MTCFGRXMJLQNBG-REOHCLBHSA-N 0.000 description 1
PMJHHCWVYXUKFD-PLNGDYQASA-N (3z)-penta-1,3-diene Chemical compound C\C=C/C=C PMJHHCWVYXUKFD-PLNGDYQASA-N 0.000 description 1
QRXMUCSWCMTJGU-UHFFFAOYSA-L (5-bromo-4-chloro-1h-indol-3-yl) phosphate Chemical compound C1=C(Br)C(Cl)=C2C(OP([O-])(=O)[O-])=CNC2=C1 QRXMUCSWCMTJGU-UHFFFAOYSA-L 0.000 description 1
OYHQOLUKZRVURQ-NTGFUMLPSA-N (9Z,12Z)-9,10,12,13-tetratritiooctadeca-9,12-dienoic acid Chemical compound C(CCCCCCC\C(=C(/C\C(=C(/CCCCC)\[3H])\[3H])\[3H])\[3H])(=O)O OYHQOLUKZRVURQ-NTGFUMLPSA-N 0.000 description 1
101150084750 1 gene Proteins 0.000 description 1
JDSRHVWSAMTSSN-IRQZEAMPSA-N 13(S)-HPODE Chemical compound CCCCC[C@H](OO)\C=C\C=C/CCCCCCCC(O)=O JDSRHVWSAMTSSN-IRQZEAMPSA-N 0.000 description 1
FOWNDZJYGGTHRO-DKWTVANSSA-N 2-aminoacetic acid;(2s)-2-aminobutanedioic acid Chemical class NCC(O)=O.OC(=O)[C@@H](N)CC(O)=O FOWNDZJYGGTHRO-DKWTVANSSA-N 0.000 description 1
241000589158 Agrobacterium Species 0.000 description 1
241001677738 Aleuron Species 0.000 description 1
108020005544 Antisense RNA Proteins 0.000 description 1
DCXYFEDJOCDNAF-UHFFFAOYSA-N Asparagine Natural products OC(=O)C(N)CC(N)=O DCXYFEDJOCDNAF-UHFFFAOYSA-N 0.000 description 1
CKLJMWTZIZZHCS-UHFFFAOYSA-N Aspartic acid Chemical compound OC(=O)C(N)CC(O)=O CKLJMWTZIZZHCS-UHFFFAOYSA-N 0.000 description 1
241000228212 Aspergillus Species 0.000 description 1
241000894006 Bacteria Species 0.000 description 1
VEJPZXZKCZQONO-UHFFFAOYSA-N CCCCCCCCCC(O)CCCC=CC=CC(=O)OO Chemical compound CCCCCCCCCC(O)CCCC=CC=CC(=O)OO VEJPZXZKCZQONO-UHFFFAOYSA-N 0.000 description 1
108020004705 Codon Proteins 0.000 description 1
230000009946 DNA mutation Effects 0.000 description 1
238000001712 DNA sequencing Methods 0.000 description 1
RWSOTUBLDIXVET-UHFFFAOYSA-N Dihydrogen sulfide Chemical class S RWSOTUBLDIXVET-UHFFFAOYSA-N 0.000 description 1
238000002965 ELISA Methods 0.000 description 1
LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
206010064571 Gene mutation Diseases 0.000 description 1
206010020649 Hyperkeratosis Diseases 0.000 description 1
DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 description 1
ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 description 1
AYFVYJQAPQTCCC-GBXIJSLDSA-N L-threonine Chemical compound C[C@@H](O)[C@H](N)C(O)=O AYFVYJQAPQTCCC-GBXIJSLDSA-N 0.000 description 1
QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 1
OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 1
108091026898 Leader sequence (mRNA) Proteins 0.000 description 1
239000000867 Lipoxygenase Inhibitor Substances 0.000 description 1
229940122142 Lipoxygenase inhibitor Drugs 0.000 description 1
231100000678 Mycotoxin Toxicity 0.000 description 1
239000000020 Nitrocellulose Substances 0.000 description 1
WRRQLBKKJXSSCZ-UHFFFAOYSA-N OOOC(CCCCCCCC)CCCCCCCCC Chemical compound OOOC(CCCCCCCC)CCCCCCCCC WRRQLBKKJXSSCZ-UHFFFAOYSA-N 0.000 description 1
241000283973 Oryctolagus cuniculus Species 0.000 description 1
238000002944 PCR assay Methods 0.000 description 1
108010002747 Pfu DNA polymerase Proteins 0.000 description 1
108700001094 Plant Genes Proteins 0.000 description 1
229920001213 Polysorbate 20 Polymers 0.000 description 1
LCTONWCANYUPML-UHFFFAOYSA-M Pyruvate Chemical compound CC(=O)C([O-])=O LCTONWCANYUPML-UHFFFAOYSA-M 0.000 description 1
239000013614 RNA sample Substances 0.000 description 1
108700005079 Recessive Genes Proteins 0.000 description 1
102000052708 Recessive Genes Human genes 0.000 description 1
101100473189 Schizosaccharomyces pombe (strain 972 / ATCC 24843) rpn1 gene Proteins 0.000 description 1
229940124639 Selective inhibitor Drugs 0.000 description 1
MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 description 1
DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 1
108010073771 Soybean Proteins Proteins 0.000 description 1
108091081024 Start codon Proteins 0.000 description 1
NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
LSNNMFCWUKXFEE-UHFFFAOYSA-N Sulfurous acid Chemical class OS(O)=O LSNNMFCWUKXFEE-UHFFFAOYSA-N 0.000 description 1
108010006785 Taq Polymerase Proteins 0.000 description 1
108091036066 Three prime untranslated region Proteins 0.000 description 1
AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 description 1
239000004473 Threonine Substances 0.000 description 1
QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 1
238000002835 absorbance Methods 0.000 description 1
230000001133 acceleration Effects 0.000 description 1
238000009825 accumulation Methods 0.000 description 1
IKHGUXGNUITLKF-XPULMUKRSA-N acetaldehyde Chemical compound [14CH]([14CH3])=O IKHGUXGNUITLKF-XPULMUKRSA-N 0.000 description 1
108020002494 acetyltransferase Proteins 0.000 description 1
102000005421 acetyltransferase Human genes 0.000 description 1
150000007513 acids Chemical class 0.000 description 1
230000004075 alteration Effects 0.000 description 1
229910021529 ammonia Inorganic materials 0.000 description 1
239000003963 antioxidant agent Substances 0.000 description 1
230000003078 antioxidant effect Effects 0.000 description 1
235000006708 antioxidants Nutrition 0.000 description 1
125000003118 aryl group Chemical group 0.000 description 1
229960001230 asparagine Drugs 0.000 description 1
235000009582 asparagine Nutrition 0.000 description 1
238000002820 assay format Methods 0.000 description 1
244000052616 bacterial pathogen Species 0.000 description 1
101150103518 bar gene Proteins 0.000 description 1
230000008901 benefit Effects 0.000 description 1
238000002306 biochemical method Methods 0.000 description 1
230000003115 biocidal effect Effects 0.000 description 1
230000008033 biological extinction Effects 0.000 description 1
230000033228 biological regulation Effects 0.000 description 1
244000309466 calf Species 0.000 description 1
229910052799 carbon Inorganic materials 0.000 description 1
238000012512 characterization method Methods 0.000 description 1
239000007795 chemical reaction product Substances 0.000 description 1
239000003795 chemical substances by application Substances 0.000 description 1
238000010367 cloning Methods 0.000 description 1
238000000658 coextraction Methods 0.000 description 1
230000000295 complement effect Effects 0.000 description 1
239000003184 complementary RNA Substances 0.000 description 1
238000012790 confirmation Methods 0.000 description 1
238000011109 contamination Methods 0.000 description 1
230000001276 controlling effect Effects 0.000 description 1
238000001816 cooling Methods 0.000 description 1
239000000287 crude extract Substances 0.000 description 1
230000002950 deficient Effects 0.000 description 1
238000012217 deletion Methods 0.000 description 1
230000037430 deletion Effects 0.000 description 1
230000001419 dependent effect Effects 0.000 description 1
238000001212 derivatisation Methods 0.000 description 1
239000003599 detergent Substances 0.000 description 1
SWULYVBHRSPZCK-UHFFFAOYSA-N diazonio(1,2,3-trihydroxypropyl)azanide Chemical compound OCC(O)C(O)[N-][N+]#N SWULYVBHRSPZCK-UHFFFAOYSA-N 0.000 description 1
239000005546 dideoxynucleotide Substances 0.000 description 1
150000001993 dienes Chemical class 0.000 description 1
230000004069 differentiation Effects 0.000 description 1
238000010790 dilution Methods 0.000 description 1
239000012895 dilution Substances 0.000 description 1
235000021186 dishes Nutrition 0.000 description 1
238000009300 dissolved air flotation Methods 0.000 description 1
210000005069 ears Anatomy 0.000 description 1
235000013399 edible fruits Nutrition 0.000 description 1
238000001962 electrophoresis Methods 0.000 description 1
238000007824 enzymatic assay Methods 0.000 description 1
230000009088 enzymatic function Effects 0.000 description 1
238000006911 enzymatic reaction Methods 0.000 description 1
238000001952 enzyme assay Methods 0.000 description 1
230000029142 excretion Effects 0.000 description 1
239000013613 expression plasmid Substances 0.000 description 1
239000011536 extraction buffer Substances 0.000 description 1
238000001125 extrusion Methods 0.000 description 1
239000000835 fiber Substances 0.000 description 1
230000037433 frameshift Effects 0.000 description 1
230000006870 function Effects 0.000 description 1
238000004817 gas chromatography Methods 0.000 description 1
239000011521 glass Substances 0.000 description 1
ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 1
150000002333 glycines Chemical class 0.000 description 1
230000012010 growth Effects 0.000 description 1
238000010438 heat treatment Methods 0.000 description 1
230000002363 herbicidal effect Effects 0.000 description 1
239000004009 herbicide Substances 0.000 description 1
150000002432 hydroperoxides Chemical class 0.000 description 1
RWSOTUBLDIXVET-UHFFFAOYSA-M hydrosulfide Chemical compound [SH-] RWSOTUBLDIXVET-UHFFFAOYSA-M 0.000 description 1
239000012133 immunoprecipitate Substances 0.000 description 1
230000006872 improvement Effects 0.000 description 1
230000002779 inactivation Effects 0.000 description 1
239000004615 ingredient Substances 0.000 description 1
150000002576 ketones Chemical class 0.000 description 1
PRFXRIUZNKLRHM-UHFFFAOYSA-N l-prostaglandin B2 Natural products CCCCCC(O)C=CC1=C(CC=CCCCC(O)=O)C(=O)CC1 PRFXRIUZNKLRHM-UHFFFAOYSA-N 0.000 description 1
235000015095 lager Nutrition 0.000 description 1
239000003446 ligand Substances 0.000 description 1
230000000670 limiting effect Effects 0.000 description 1
230000007774 longterm Effects 0.000 description 1
230000014759 maintenance of location Effects 0.000 description 1
210000004962 mammalian cell Anatomy 0.000 description 1
239000003550 marker Substances 0.000 description 1
238000005360 mashing Methods 0.000 description 1
238000012986 modification Methods 0.000 description 1
230000004048 modification Effects 0.000 description 1
238000010369 molecular cloning Methods 0.000 description 1
238000012544 monitoring process Methods 0.000 description 1
230000036438 mutation frequency Effects 0.000 description 1
239000002636 mycotoxin Substances 0.000 description 1
ZIUHHBKFKCYYJD-UHFFFAOYSA-N n,n'-methylenebisacrylamide Chemical compound C=CC(=O)NCNC(=O)C=C ZIUHHBKFKCYYJD-UHFFFAOYSA-N 0.000 description 1
UMFJAHHVKNCGLG-UHFFFAOYSA-N n-Nitrosodimethylamine Chemical compound CN(C)N=O UMFJAHHVKNCGLG-UHFFFAOYSA-N 0.000 description 1
JPXMTWWFLBLUCD-UHFFFAOYSA-N nitro blue tetrazolium(2+) Chemical compound COC1=CC(C=2C=C(OC)C(=CC=2)[N+]=2N(N=C(N=2)C=2C=CC=CC=2)C=2C=CC(=CC=2)[N+]([O-])=O)=CC=C1[N+]1=NC(C=2C=CC=CC=2)=NN1C1=CC=C([N+]([O-])=O)C=C1 JPXMTWWFLBLUCD-UHFFFAOYSA-N 0.000 description 1
229920001220 nitrocellulos Polymers 0.000 description 1
108010058731 nopaline synthase Proteins 0.000 description 1
235000016709 nutrition Nutrition 0.000 description 1
230000035764 nutrition Effects 0.000 description 1
230000036284 oxygen consumption Effects 0.000 description 1
239000000123 paper Substances 0.000 description 1
230000036961 partial effect Effects 0.000 description 1
244000052769 pathogen Species 0.000 description 1
239000008188 pellet Substances 0.000 description 1
HGBOYTHUEUWSSQ-UHFFFAOYSA-N pentanal Chemical class CCCCC=O HGBOYTHUEUWSSQ-UHFFFAOYSA-N 0.000 description 1
239000008363 phosphate buffer Substances 0.000 description 1
108010082527 phosphinothricin N-acetyltransferase Proteins 0.000 description 1
230000035479 physiological effects, processes and functions Effects 0.000 description 1
238000011020 pilot scale process Methods 0.000 description 1
238000002264 polyacrylamide gel electrophoresis Methods 0.000 description 1
239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 1
235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 1
NLKNQRATVPKPDG-UHFFFAOYSA-M potassium iodide Chemical class [K+].[I-] NLKNQRATVPKPDG-UHFFFAOYSA-M 0.000 description 1
238000001556 precipitation Methods 0.000 description 1
ULWHHBHJGPPBCO-UHFFFAOYSA-N propane-1,1-diol Chemical compound CCC(O)O ULWHHBHJGPPBCO-UHFFFAOYSA-N 0.000 description 1
PRFXRIUZNKLRHM-OSJNIVAESA-N prostaglandin b2 Chemical compound CCCCC[C@H](O)\C=C\C1=C(C\C=C\CCCC(O)=O)C(=O)CC1 PRFXRIUZNKLRHM-OSJNIVAESA-N 0.000 description 1
238000000159 protein binding assay Methods 0.000 description 1
238000003753 real-time PCR Methods 0.000 description 1
238000006479 redox reaction Methods 0.000 description 1
230000003252 repetitive effect Effects 0.000 description 1
230000010076 replication Effects 0.000 description 1
238000011160 research Methods 0.000 description 1
229920006395 saturated elastomer Polymers 0.000 description 1
239000006152 selective media Substances 0.000 description 1
238000000926 separation method Methods 0.000 description 1
210000002966 serum Anatomy 0.000 description 1
239000007790 solid phase Substances 0.000 description 1
239000000243 solution Substances 0.000 description 1
239000002904 solvent Substances 0.000 description 1
229940001941 soy protein Drugs 0.000 description 1
241000894007 species Species 0.000 description 1
239000007921 spray Substances 0.000 description 1
125000004079 stearyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
239000010902 straw Substances 0.000 description 1
229910052717 sulfur Inorganic materials 0.000 description 1
239000011593 sulfur Substances 0.000 description 1
238000010998 test method Methods 0.000 description 1
YLQBMQCUIZJEEH-UHFFFAOYSA-N tetrahydrofuran Natural products C=1C=COC=1 YLQBMQCUIZJEEH-UHFFFAOYSA-N 0.000 description 1
238000004448 titration Methods 0.000 description 1
238000001890 transfection Methods 0.000 description 1
UFTFJSFQGQCHQW-UHFFFAOYSA-N triformin Chemical compound O=COCC(OC=O)COC=O UFTFJSFQGQCHQW-UHFFFAOYSA-N 0.000 description 1
OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 1
238000011144 upstream manufacturing Methods 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12C—BEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
- C12C12/00—Processes specially adapted for making special kinds of beer
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12C—BEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
- C12C1/00—Preparation of malt
- C12C1/18—Preparation of malt extract or of special kinds of malt, e.g. caramel, black malt
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
- C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y113/00—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
- C12Y113/11—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of two atoms of oxygen (1.13.11)
- C12Y113/11012—Linoleate 13S-lipoxygenase (1.13.11.12)
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12C—BEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
- C12C2200/00—Special features
- C12C2200/01—Use of specific genetic variants of barley or other sources of fermentable carbohydrates for beer brewing

Landscapes

Life Sciences & Earth Sciences (AREA)
Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Engineering & Computer Science (AREA)
Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
Bioinformatics & Cheminformatics (AREA)
General Engineering & Computer Science (AREA)
Biotechnology (AREA)
Biochemistry (AREA)
General Health & Medical Sciences (AREA)
Molecular Biology (AREA)
Biomedical Technology (AREA)
Microbiology (AREA)
Food Science & Technology (AREA)
Plant Pathology (AREA)
Cell Biology (AREA)
Physics & Mathematics (AREA)
Biophysics (AREA)
Nutrition Science (AREA)
Oil, Petroleum & Natural Gas (AREA)
Medicinal Chemistry (AREA)
Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Enzymes And Modification Thereof (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Cereal-Derived Products (AREA)
Peptides Or Proteins (AREA)
Non-Alcoholic Beverages (AREA)

SK937-2003A 2000-12-29 2001-01-22 Low-lipoxygenase 1 barley SK9372003A3 (en)

Applications Claiming Priority (3)

Application Number	Priority Date	Filing Date	Title
US09/751,687 US6660915B2 (en)	2000-12-29	2000-12-29	Low lipoxygenase 1 barley
PCT/IB2000/002045 WO2002053720A1 (en)	2000-12-29	2000-12-29	Low-lipoxygenase 1 barley
PCT/IB2001/000207 WO2002053721A1 (en)	2000-12-29	2001-01-22	Low-lipoxygenase 1 barley

Publications (1)

Publication Number	Publication Date
SK9372003A3 true SK9372003A3 (en)	2004-05-04

Family

ID=26318622

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
SK937-2003A SK9372003A3 (en)	2000-12-29	2001-01-22	Low-lipoxygenase 1 barley

Country Status (19)

Country	Link
EP (2)	EP2305797A3 (cs)
JP (1)	JP5004402B2 (cs)
CN (1)	CN100372930C (cs)
AT (1)	ATE531794T1 (cs)
BG (1)	BG66243B1 (cs)
BR (1)	BR0116579A (cs)
CA (1)	CA2433250C (cs)
CZ (1)	CZ298689B6 (cs)
DK (1)	DK1346030T3 (cs)
EA (1)	EA007955B1 (cs)
EE (1)	EE05567B1 (cs)
ES (1)	ES2376415T3 (cs)
HU (1)	HUP0401290A3 (cs)
NZ (1)	NZ527171A (cs)
PL (1)	PL208246B1 (cs)
PT (1)	PT1346030E (cs)
SK (1)	SK9372003A3 (cs)
UA (1)	UA88130C2 (cs)
WO (1)	WO2002053721A1 (cs)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
JP4113795B2 (ja) *	2003-03-25	2008-07-09	サッポロビール株式会社	大麦リポキシゲナーゼ−１遺伝子、大麦の選抜方法、麦芽アルコール飲料用原料及び麦芽アルコール飲料の製造方法
US7420105B2 (en) *	2004-03-11	2008-09-02	Carlsberg A/S	Barley for production of flavor-stable beverage
US9587210B2 (en)	2008-12-03	2017-03-07	Carlsberg Breweries A/S	Energy saving brewing method
MX2011005619A (es)	2008-12-03	2011-09-09	Carlsberg Breweries As	Bebidas derivadas de cebada y malta con niveles bajos de sulfuro de dimetilo.
SA109310019B1 (ar)	2008-12-30	2014-09-10	Carlsberg Breweries As	شعير له نشاط ليبوأوكسجيناز منخفض
WO2011074964A1 (en) *	2009-12-18	2011-06-23	Keygene N.V.	Improved bulked mutant analysis
CN103209584B (zh)	2010-06-03	2014-10-29	嘉士伯酿酒有限公司	节能酿造方法
JP2017205038A (ja) *	2016-05-17	2017-11-24	サッポロビール株式会社	ビールテイスト飲料、ビールテイスト飲料の製造方法、及びビールテイスト飲料の香味向上方法
JP2017205036A (ja) *	2016-05-17	2017-11-24	サッポロビール株式会社	ビールテイスト飲料、ビールテイスト飲料の製造方法、及びビールテイスト飲料の香味向上方法
JP6691826B2 (ja) *	2016-06-01	2020-05-13	サッポロビール株式会社	蒸留酒類
RS67705B1 (sr)	2016-07-01	2026-02-27	Carlsberg Breweries As	Prerađena pića zasnovana na žitaricama
CN106405024B (zh) *	2016-08-25	2018-08-24	青岛啤酒股份有限公司	一种评价麦芽脂质氧化程度的方法
ES2986024T3 (es)	2017-12-28	2024-11-08	Carlsberg As	Métodos rápidos para preparar extractos de cereales
UA128208C2 (uk)	2017-12-28	2024-05-08	Карлсберг А/С	Спосіб одержання водного екстракту зернової культури та спосіб одержання напою
EP3784058A1 (en)	2018-04-25	2021-03-03	Carlsberg A/S	Barley based beverages
CN111718974B (zh) *	2020-06-29	2021-09-24	中食都庆（山东）生物技术有限公司	绿豆肽在制备促排铅的药物中的应用、活性肽及其应用

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
CA1339684C (en)	1988-05-17	1998-02-24	Peter H. Quail	Plant ubquitin promoter system
AU7443596A (en) *	1995-10-13	1997-04-30	Purdue Research Foundation	Improvement of fruit quality by inhibiting production of lipoxygenase in fruits
US5844121A (en)	1996-01-19	1998-12-01	The Texas A & M University System	Method of inhibiting mycotoxin production in seed crops by modifying lipoxygenase pathway genes

2001
- 2001-01-22 EP EP10011413A patent/EP2305797A3/en not_active Withdrawn
- 2001-01-22 SK SK937-2003A patent/SK9372003A3/sk unknown
- 2001-01-22 PT PT01902597T patent/PT1346030E/pt unknown
- 2001-01-22 CZ CZ20031872A patent/CZ298689B6/cs not_active IP Right Cessation
- 2001-01-22 UA UA2003077122A patent/UA88130C2/uk unknown
- 2001-01-22 NZ NZ527171A patent/NZ527171A/en not_active IP Right Cessation
- 2001-01-22 CA CA2433250A patent/CA2433250C/en not_active Expired - Fee Related
- 2001-01-22 CN CNB01822489XA patent/CN100372930C/zh not_active Expired - Fee Related
- 2001-01-22 WO PCT/IB2001/000207 patent/WO2002053721A1/en not_active Ceased
- 2001-01-22 HU HU0401290A patent/HUP0401290A3/hu unknown
- 2001-01-22 BR BR0116579-8A patent/BR0116579A/pt not_active Application Discontinuation
- 2001-01-22 PL PL363480A patent/PL208246B1/pl not_active IP Right Cessation
- 2001-01-22 AT AT01902597T patent/ATE531794T1/de active
- 2001-01-22 EA EA200300743A patent/EA007955B1/ru not_active IP Right Cessation
- 2001-01-22 JP JP2002555231A patent/JP5004402B2/ja not_active Expired - Fee Related
- 2001-01-22 EP EP01902597A patent/EP1346030B1/en not_active Expired - Lifetime
- 2001-01-22 DK DK01902597.2T patent/DK1346030T3/da active
- 2001-01-22 EE EEP200300257A patent/EE05567B1/xx not_active IP Right Cessation
- 2001-01-22 ES ES01902597T patent/ES2376415T3/es not_active Expired - Lifetime
2003
- 2003-07-04 BG BG107971A patent/BG66243B1/bg unknown

Also Published As

Publication number	Publication date
HUP0401290A2 (hu)	2004-09-28
WO2002053721A1 (en)	2002-07-11
DK1346030T3 (da)	2012-02-13
EP1346030B1 (en)	2011-11-02
UA88130C2 (uk)	2009-09-25
PT1346030E (pt)	2012-02-08
NZ527171A (en)	2005-07-29
CA2433250A1 (en)	2002-07-11
HUP0401290A3 (en)	2005-06-28
CA2433250C (en)	2015-08-11
EP2305797A3 (en)	2011-07-13
CZ298689B6 (cs)	2007-12-19
BR0116579A (pt)	2004-04-20
ES2376415T3 (es)	2012-03-13
PL363480A1 (en)	2004-11-15
EA200300743A1 (ru)	2004-02-26
CN1487995A (zh)	2004-04-07
JP2004522434A (ja)	2004-07-29
CN100372930C (zh)	2008-03-05
EE200300257A (et)	2003-10-15
PL208246B1 (pl)	2011-04-29
ATE531794T1 (de)	2011-11-15
BG107971A (bg)	2004-09-30
EA007955B1 (ru)	2007-02-27
EP2305797A2 (en)	2011-04-06
EE05567B1 (et)	2012-08-15
JP5004402B2 (ja)	2012-08-22
CZ20031872A3 (cs)	2003-11-12
BG66243B1 (bg)	2012-08-31
EP1346030A1 (en)	2003-09-24

Legal Events

Date	Code	Title	Description
2017-05-03	FB9A	Suspension of patent application procedure

Publication	Publication Date	Title
CN1981041B (zh)	2011-03-23	产生风味稳定饮料的大麦
CN102333440B (zh)	2014-07-23	脂加氧酶活性降低的大麦
AU2009321894B2 (en)	2014-07-31	Barley and malt-derived beverages with low dimethyl sulfide level
JP5004402B2 (ja)	2012-08-22	低リポキシゲナーゼ１オオムギ
JP6007174B2 (ja)	2016-10-12	エネルギーを節約した醸造法
US6660915B2 (en)	2003-12-09	Low lipoxygenase 1 barley
AU2001230454B2 (en)	2007-02-22	Low-lipoxygenase 1 Barley
WO2002053720A1 (en)	2002-07-11	Low-lipoxygenase 1 barley
AU2001230454A1 (en)	2003-01-23	Low-lipoxygenase 1 Barley
HK1162851B (en)	2017-07-14	Barley with reduced lipoxygenase activity and beverage prepared therefrom