UA77642C2 - Human hybrid cell for expression of gene of mammals, human cell line hkb11, method for producing hybrid human cells suitable for expression of heterologic protein - Google Patents

Human hybrid cell for expression of gene of mammals, human cell line hkb11, method for producing hybrid human cells suitable for expression of heterologic protein Download PDF

Info

Publication number: UA77642C2
Authority: UA; Ukraine
Prior art keywords: cells; cell; human; protein; cell line
Prior art date: 1998-12-10

Application number

UA2001074776A

Other languages

English (en)

Ukrainian (uk)

Original Assignee

Bayer Corp

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1998-12-10

Filing date

1999-08-12

Publication date

2007-01-15

1999-08-12 Application filed by Bayer Corp filed Critical Bayer Corp

2007-01-15 Publication of UA77642C2 publication Critical patent/UA77642C2/uk

Links

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Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/02—Preparation of hybrid cells by fusion of two or more cells, e.g. protoplast fusion
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/10—Cells modified by introduction of foreign genetic material
- C12N5/12—Fused cells, e.g. hybridomas
- C12N5/16—Animal cells
- C12N5/163—Animal cells one of the fusion partners being a B or a T lymphocyte
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2510/00—Genetically modified cells
- C12N2510/02—Cells for production

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Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Engineering & Computer Science (AREA)
Genetics & Genomics (AREA)
Chemical & Material Sciences (AREA)
Biotechnology (AREA)
Organic Chemistry (AREA)
Bioinformatics & Cheminformatics (AREA)
Biomedical Technology (AREA)
Wood Science & Technology (AREA)
Zoology (AREA)
General Engineering & Computer Science (AREA)
General Health & Medical Sciences (AREA)
Biochemistry (AREA)
Microbiology (AREA)
Cell Biology (AREA)
Immunology (AREA)
Molecular Biology (AREA)
Biophysics (AREA)
Physics & Mathematics (AREA)
Plant Pathology (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Peptides Or Proteins (AREA)
Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

UA2001074776A 1998-12-10 1999-08-12 Human hybrid cell for expression of gene of mammals, human cell line hkb11, method for producing hybrid human cells suitable for expression of heterologic protein UA77642C2 (en)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
US09/209,920 US6136599A (en)	1998-12-10	1998-12-10	Human hybrid host cell for mammalian gene expression
PCT/US1999/029332 WO2000034462A1 (en)	1998-12-10	1999-12-08	Human hybrid host cell for mammalian gene expression

Publications (1)

Publication Number	Publication Date
UA77642C2 true UA77642C2 (en)	2007-01-15

Family

ID=22780882

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
UA2001074776A UA77642C2 (en)	1998-12-10	1999-08-12	Human hybrid cell for expression of gene of mammals, human cell line hkb11, method for producing hybrid human cells suitable for expression of heterologic protein

Country Status (27)

Country	Link
US (1)	US6136599A (id)
EP (1)	EP1137768B1 (id)
JP (3)	JP4332300B2 (id)
KR (1)	KR100627753B1 (id)
CN (1)	CN1230532C (id)
AT (1)	ATE306547T1 (id)
AU (1)	AU757188B2 (id)
BG (1)	BG65263B1 (id)
BR (1)	BRPI9916095B8 (id)
CA (1)	CA2353698C (id)
CU (2)	CU23382A3 (id)
CZ (1)	CZ302677B6 (id)
DE (1)	DE69927707T2 (id)
DK (1)	DK1137768T3 (id)
ES (1)	ES2249929T3 (id)
HU (1)	HU229775B1 (id)
ID (1)	ID30448A (id)
IL (2)	IL143351A0 (id)
NO (1)	NO330548B1 (id)
PL (1)	PL205549B1 (id)
RO (1)	RO121563B1 (id)
RU (1)	RU2228355C2 (id)
SI (1)	SI20693B (id)
SK (1)	SK287888B6 (id)
TR (1)	TR200101642T2 (id)
UA (1)	UA77642C2 (id)
WO (1)	WO2000034462A1 (id)

Families Citing this family (26)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US6358703B1 (en)	1998-12-10	2002-03-19	Bayer Corporation	Expression system for factor VIII
US6136599A (en) *	1998-12-10	2000-10-24	Bayer Corporation	Human hybrid host cell for mammalian gene expression
US6348192B1 (en) *	1999-05-11	2002-02-19	Bayer Corporation	Interleukin-2 mutein expressed from mammalian cells
US20040235715A1 (en) *	1999-11-12	2004-11-25	Bayer Corporation	Method of producing glycosylated bikunin
US6569657B1 (en) *	2000-06-27	2003-05-27	Millennium Pharmaceuticals, Inc.	32140, a novel human aldehyde dehydrogenase and uses therefor
US7094587B2 (en) *	2000-06-27	2006-08-22	Millennium Pharmaceuticals, Inc.	16002 Molecules and uses therefor
MY139948A (en) *	2000-09-28	2009-11-30	Bayer Corp	Enhanced transfection system
US7723102B2 (en) *	2000-09-28	2010-05-25	Bayer Corporation	Enhanced transfection system
US20060073141A1 (en) *	2001-06-28	2006-04-06	Domantis Limited	Compositions and methods for treating inflammatory disorders
KR100496861B1 (ko)	2002-09-26	2005-06-22	삼성전자주식회사	하나의 핸들러에 2개 이상의 테스트 보드를 갖는 테스트장비 및 그 테스트 방법
US7285269B2 (en)	2002-12-02	2007-10-23	Amgen Fremont, Inc.	Antibodies directed to tumor necrosis factor
US7244616B2 (en)	2003-06-27	2007-07-17	Bayer Pharmaceuticals Corporation	Use of molecular chaperones for the enhanced production of secreted, recombinant proteins in mammalian cells
PT2363414T (pt)	2004-11-12	2022-08-04	Bayer Healthcare Llc	Modificação de fviii direcionada a sítio
JP2010528647A (ja)	2007-06-06	2010-08-26	ドマンティスリミテッド	ポリペプチド、抗体可変ドメインおよびアンタゴニスト
JP5628790B2 (ja)	2008-04-07	2014-11-19	バイエル・ヘルスケア・エルエルシー	糖タンパク質の組換え生産の方法
KR101005967B1 (ko) *	2008-04-12	2011-01-05	(주)셀트리온	우수한 재조합 단백질을 생산하기 위한 인간 숙주 세포
US9090948B2 (en)	2008-09-30	2015-07-28	Abbott Molecular Inc.	Primers and probes for detecting human papillomavirus and human beta globin sequences in test samples
CN102612556A (zh) *	2009-06-10	2012-07-25	Bts研究国际股份有限公司	制备杂交细胞/嵌合细胞的方法及其应用
US11649449B2 (en)	2015-09-03	2023-05-16	CHO Plus, Inc.	Hybrid cell lines for high level production of a target protein
WO2018017144A1 (en)	2016-07-19	2018-01-25	Brandeis University	Compositions and methods for identifying rna binding polypeptide targets
AU2019270342A1 (en)	2018-05-16	2020-10-29	Morphosys Ag	Antibodies targeting glycoprotein VI
CA3125527A1 (en)	2018-12-20	2020-06-25	Orionis Biosciences, Inc.	Particle based small molecule-protein complex trap
JP7617012B2 (ja)	2019-03-14	2025-01-17	モルフォシス・アーゲー	Ｃ５ａＲを標的とする抗体
EP3980524A4 (en) *	2019-06-10	2023-01-11	Cho Plus Inc.	Cell lines for high level production of protein-based pharmaceuticals
US20230357398A1 (en)	2020-09-24	2023-11-09	Morphosys Ag	Novel human antibodies binding to human cd3 epsilon
CN113604425B (zh) *	2021-06-22	2022-10-18	中山康天晟合生物技术有限公司	一种适应无血清培养基环境的wayne293 lvpro细胞及其应用

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
JPS6045849B2 (ja) *	1980-08-25	1985-10-12	林原健	ヒトエリトロポエチンの製造方法
JPS6030654B2 (ja) *	1980-12-31	1985-07-17	株式会社林原生物化学研究所	ヒトコロニ−刺激因子の製造方法
US6136599A (en) *	1998-12-10	2000-10-24	Bayer Corporation	Human hybrid host cell for mammalian gene expression

1998
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1999
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Also Published As

Publication number	Publication date
SK7912001A3 (en)	2002-03-05
DE69927707D1 (de)	2006-02-23
NO330548B1 (no)	2011-05-16
IL143351A (en)	2009-12-24
EP1137768A1 (en)	2001-10-04
PL205549B1 (pl)	2010-04-30
AU1938300A (en)	2000-06-26
EP1137768A4 (en)	2002-09-18
JP2014131513A (ja)	2014-07-17
HUP0104681A2 (hu)	2002-04-29
KR20020002360A (ko)	2002-01-09
TR200101642T2 (tr)	2001-11-21
IL143351A0 (en)	2002-04-21
CA2353698C (en)	2012-02-21
CN1230532C (zh)	2005-12-07
BG65263B1 (bg)	2007-10-31
SI20693B (sl)	2008-12-31
JP2002531125A (ja)	2002-09-24
ES2249929T3 (es)	2006-04-01
CZ20012023A3 (cs)	2001-10-17
KR100627753B1 (ko)	2006-09-26
SI20693A (sl)	2002-04-30
HUP0104681A3 (en)	2005-10-28
CN1339063A (zh)	2002-03-06
JP5826311B2 (ja)	2015-12-02
ATE306547T1 (de)	2005-10-15
JP2009148295A (ja)	2009-07-09
NO20012806D0 (no)	2001-06-07
DE69927707T2 (de)	2006-07-13
CU23238A1 (es)	2007-09-26
ID30448A (id)	2001-12-06
BRPI9916095B8 (pt)	2021-05-25
BR9916095A (pt)	2001-09-04
SK287888B6 (sk)	2012-03-02
CZ302677B6 (cs)	2011-08-31
RO121563B1 (ro)	2007-11-30
JP5508740B2 (ja)	2014-06-04
BG105566A (en)	2002-03-29
BRPI9916095B1 (pt)	2015-10-13
HU229775B1 (en)	2014-07-28
NO20012806L (no)	2001-06-07
CA2353698A1 (en)	2000-06-15
RU2228355C2 (ru)	2004-05-10
CU23382A3 (es)	2009-06-25
US6136599A (en)	2000-10-24
EP1137768B1 (en)	2005-10-12
WO2000034462A1 (en)	2000-06-15
AU757188B2 (en)	2003-02-06
DK1137768T3 (da)	2005-12-19
JP4332300B2 (ja)	2009-09-16

Publication	Publication Date	Title
UA77642C2 (en)	2007-01-15	Human hybrid cell for expression of gene of mammals, human cell line hkb11, method for producing hybrid human cells suitable for expression of heterologic protein
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AU600481B2 (en)	1990-08-16	Method of producing peptides, recombinant plasmid for use in the same and animal cells transformed with the same
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MXPA01005783A (es)	2001-12-13	Celula huesped hibrida de humano para expresion de gen de mamifero
Kreuzburg-Duffy et al.	1994	Establishment and characterization of murine macrophage-like cell lines following transformation with simian virus 40 DNA deleted at the origin of replication
JP2960552B2 (ja)	1999-10-06	動物細胞の育種方法
SU1495375A1 (ru)	1989-07-23	Штамм культивируемых клеток гепатокарциномы человека, дефектный по тимидинкиназе дл гибридизации и трансфекции
JPWO1994012658A1 (ja)	1995-01-05	外来蛋白質の産生方法