AR041993A1 - Variantes de subtilasa originaria de bacillus y su uso en composiciones detergentes - Google Patents

Variantes de subtilasa originaria de bacillus y su uso en composiciones detergentes

Info

Publication number
AR041993A1
AR041993A1 ARP030104052A ARP030104052A AR041993A1 AR 041993 A1 AR041993 A1 AR 041993A1 AR P030104052 A ARP030104052 A AR P030104052A AR P030104052 A ARP030104052 A AR P030104052A AR 041993 A1 AR041993 A1 AR 041993A1
Authority
AR
Argentina
Prior art keywords
variants
l96lg
compositions
detergent compositions
g61ge
Prior art date
Application number
ARP030104052A
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English (en)
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Novozymes As
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Application filed by Novozymes As filed Critical Novozymes As
Publication of AR041993A1 publication Critical patent/AR041993A1/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/582—Recycling of unreacted starting or intermediate materials

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Detergent Compositions (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La presente se refiere a variantes de subtilasa novedosas que exhiben alteraciones respecto de la subtilasa originaria en una o más propiedades incluyendo: rendimiento de lavado, estabilidad térmica, estabilidad de almacenamiento o actividad catalítica. Las variantes de la presente son adecuadas para empleo en, por ejemplo, composiciones de lavado o detergentes, tales como composiciones detergentes de lavandería y composiciones para lavado de vajilla, incluyendo composiciones para lavado de vajilla automático. Reivindicación 1: Una variante de proteasa que comprende: a) una inserción, substitución o deleción de uno de los residuos de aminoácido K, H, R, E, D, Q, N, C, V, L, I, P, M, F, W, Y, G, A, S, T en una o más de las posiciones 62,68, 97,98, 99,106, 131,170, 245,252 en combinación con al menos una de las siguientes modificaciones *0AQSVPWG; A1T,V; Q2L; S3T,A,L; V4L,A; I8V,T; S9G,D,R,K,L,V; R10H,K; V11A; Q12D; A13V; P14S,T,D,A,M,V,K,Q,L,H,R,I; A15M,T; A16P; H17R; N18S,H; R19W,K,L,F,G,I; G20*,R,A; L21F,LP,LW,LA,LG; T22S,A,K,TV,TG,TL,TW,TV,G,L,TY; G23S; S24P; K27R, V28I; V30I; I35T,V; T38S; P40L; N43D; R45H,K; G46D; A48T; S49N; F50S; V51A,I,D; P52V,A; P55S,A; S57P; G61E,D,S,R,GP; N62D,ND,NE,DE,NG,E,S; V68A,S,L,I; T71A; I72V; L75I; N76S,D; N77S; S78T; V81A; A85T; S87C; A88V,T; E89G; K94N; V95C,T; L96LA,LG; G97E,D,W,A,GG,GA,GV,N,GS; A98S,D,E,T,AS,AD,AV,AE,AH,Q,N,M,L,G,R,V,S; S99D,L,A,AD,SD,SM,SG,DA,P,G,N,C,M,V,I; G100S,GE,C; S101SA,SK; G102D,S; S103D,E,Y,L,Q,H,T; V104T,S,R,I,N,M,L,D; S106D,E,T,M,G,A,L,F,I; I107T,V,M; A108V,T,S; L111I,V; A114V; N116S,D; G118D; M119L,I,V,A,S; H120N,D,Q,K,E,Y,S; V121A; L124C; L126I; G127E; S128N,I,G,C; P129PSN,T,E,D,S,N,A; S130P,T,C,*; P131M,F,W,L,A,H,T,*,PA,S,Q,R,E,G,D,C; S132G,T; A133ASA; T134A; Q137H,E,D; A138G,V; V139L,I; N140D,K; T143A; S144D,N,P; R145G; V150I; A151V,G; A152P; A158T,V,C,E,L,D,M; G160A,D; S163G,C,N,A; Y167K,A,I; A168G; A169G; R170C,S,H,L; Y171C; A172V; N173D; A174V; M175L,I,V,A,S,T; N183D; N184D,S; N185S,D; R186L,C,H; S188G; S190A; Y192H; G195F,E; V203S,A,L,Q,M,F,I; N204T,D,S; Q206L; Y209C,H; G211D; S212N,L; T213A; Y214C,H; A215D,T; N218D,S; M222L,I,V,A,S; A223G; T224A,S; A228T; A230V; A232S,L,T,P; V234I; Q236A,L,D,T,C,M,F,S; K237R; N238D; P239T,S; S240F; S242T; V244I,M,A; Q245R,K,E,D,T,F,N,V,W,G,I,S,C,L,A,M; N248P,D,S; K251E,R; N252G,H,D,V,M,S,T,E,Y,S,Q,K,A,L; A254S; T255A,S; S256N,R,G; L257G; G258K, S259A,N,G; T260A,R; N261D; L262S,Q,V; Y263H,F; G264E; S265G,R,N; V268L,I; N269T; N296K; E271A; T274S,L,A,R, o b) una de las variantes de combinación siguientes A108T+L111V; L124I+S125A; P129S+S130AT; L96LA+A151G+V203A; S49N+V203L+N218D; S3T+A16P+R45C+G100S+A230V; I8V+R19K+V139I; N76D+A174AL+A194P+A230V; N185R; N62NE; H120Q+Q137E, G61GE, G61GS, G100L, A133D, V68A, N123D, L111F+Y263H, V11A+G61GE+V227A+S240F, A133E+S144K+N218D, S128A+P129S+S130SP, S9R+A15T+T22TQ+S101P, S9R+A15T+H120R+Q137D+N173S, G97E, Q245W, S9R+A15T+L96LG+Q137E+Y209H, S9R+A15T+L111V+Q137E+G211D, S9R+A15T+L111I+Q137E, S9R+A15T+L111I+H120N+Q137E, S9R+A15T+L96LG+H120Q+Q137E, S9R+A15T+T260M, S9R+A15T, Q245I, S9R+A15T+H120G+Q137E+N218D, S9R+A15T+S130P, Q245F, S9R+A15T+N218D, G63E+N76D+A194P+A230V, S9R+A15T+T224A, G100S, S9R+A15T+D60DG, A138V+V139I+A194P+N218D+A230V, A108V+A169G+R170A+Y171H, I8V+P14L+R19L+V30I+I35V+S57P+P129S+Q137D+S144D+S256N, A133D+T134S+Q137A, Q137D, A98AH, V51D, Q12E+P14L+A15T, G63E+N76D+A194P+A230V, Q12E+P14L+A15T, G97GS, V51A+S163T; V139I+A151G; S9R+A15T+L96LG+S130*; A169G+R170H o c) una o más modificaciones en la posición 68, en donde dicha(s) modificación/modificaciones comprende(n): delación, inserción y/o substitución de un residuo de aminoácido seleccionado del grupo que consiste en K, H, R, E, D, Q, N, C, V, L, I, P, M, F, W, Y, G, A, S y T.
ARP030104052A 2002-11-06 2003-11-05 Variantes de subtilasa originaria de bacillus y su uso en composiciones detergentes AR041993A1 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DKPA200201705 2002-11-06
DKPA200201933 2002-12-18
US50753703P 2003-10-01 2003-10-01

Publications (1)

Publication Number Publication Date
AR041993A1 true AR041993A1 (es) 2005-06-08

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ARP030104052A AR041993A1 (es) 2002-11-06 2003-11-05 Variantes de subtilasa originaria de bacillus y su uso en composiciones detergentes
ARP100102589A AR077318A2 (es) 2002-11-06 2010-07-15 Variantes de subtilasa

Family Applications After (1)

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ARP100102589A AR077318A2 (es) 2002-11-06 2010-07-15 Variantes de subtilasa

Country Status (13)

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EP (4) EP2138574B1 (es)
JP (3) JP4694964B2 (es)
KR (4) KR20140021051A (es)
CN (3) CN105018451A (es)
AR (2) AR041993A1 (es)
AU (1) AU2003277836B2 (es)
BR (1) BR0315916A (es)
CA (1) CA2503965C (es)
CL (1) CL2010000493A1 (es)
MX (1) MXPA05004755A (es)
MY (1) MY140915A (es)
TW (1) TWI319007B (es)
WO (1) WO2004041979A2 (es)

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EP2138574A3 (en) 2010-08-11
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AR077318A2 (es) 2011-08-17
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CA2503965C (en) 2013-10-08
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