ATE169683T1 - Verfahren zur amplifikation von nukleinsäuren auf einer festphase und reagenziensatz dafür - Google Patents

Verfahren zur amplifikation von nukleinsäuren auf einer festphase und reagenziensatz dafür

Info

Publication number
ATE169683T1
ATE169683T1 AT95936616T AT95936616T ATE169683T1 AT E169683 T1 ATE169683 T1 AT E169683T1 AT 95936616 T AT95936616 T AT 95936616T AT 95936616 T AT95936616 T AT 95936616T AT E169683 T1 ATE169683 T1 AT E169683T1
Authority
AT
Austria
Prior art keywords
nucleic acids
solid phase
amplification
reagent set
disclosed
Prior art date
Application number
AT95936616T
Other languages
English (en)
Inventor
Christine Peponnet
Original Assignee
Genset Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genset Sa filed Critical Genset Sa
Application granted granted Critical
Publication of ATE169683T1 publication Critical patent/ATE169683T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6846—Common amplification features

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
AT95936616T 1994-10-28 1995-10-27 Verfahren zur amplifikation von nukleinsäuren auf einer festphase und reagenziensatz dafür ATE169683T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR9412972A FR2726286B1 (fr) 1994-10-28 1994-10-28 Procede d'amplification d'acides nucleiques en phase solide et trousse de reactifs utile pour la mise en oeuvre de ce procede

Publications (1)

Publication Number Publication Date
ATE169683T1 true ATE169683T1 (de) 1998-08-15

Family

ID=9468346

Family Applications (1)

Application Number Title Priority Date Filing Date
AT95936616T ATE169683T1 (de) 1994-10-28 1995-10-27 Verfahren zur amplifikation von nukleinsäuren auf einer festphase und reagenziensatz dafür

Country Status (9)

Country Link
US (2) US6274351B1 (de)
EP (1) EP0787205B1 (de)
JP (1) JP4067057B2 (de)
AT (1) ATE169683T1 (de)
DE (1) DE69504094T2 (de)
DK (1) DK0787205T3 (de)
ES (1) ES2122692T3 (de)
FR (1) FR2726286B1 (de)
WO (1) WO1996013609A1 (de)

Families Citing this family (62)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7014992B1 (en) 1996-11-05 2006-03-21 Clinical Micro Sensors, Inc. Conductive oligomers attached to electrodes and nucleoside analogs
US7045285B1 (en) 1996-11-05 2006-05-16 Clinical Micro Sensors, Inc. Electronic transfer moieties attached to peptide nucleic acids
GB9624165D0 (en) 1996-11-19 1997-01-08 Amdex A S Use of nucleic acids bound to carrier macromolecules
JP2002503954A (ja) 1997-04-01 2002-02-05 グラクソ、グループ、リミテッド 核酸増幅法
US6511803B1 (en) 1997-10-10 2003-01-28 President And Fellows Of Harvard College Replica amplification of nucleic acid arrays
US6485944B1 (en) * 1997-10-10 2002-11-26 President And Fellows Of Harvard College Replica amplification of nucleic acid arrays
US6322968B1 (en) 1997-11-21 2001-11-27 Orchid Biosciences, Inc. De novo or “universal” sequencing array
US6780591B2 (en) 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US7875440B2 (en) 1998-05-01 2011-01-25 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US6872521B1 (en) 1998-06-16 2005-03-29 Beckman Coulter, Inc. Polymerase signaling assay
DE19848403A1 (de) * 1998-10-21 2000-04-27 Guenter Von Kiedrowski Verfahren zur exponentiellen Amplifikation molekularer Matrizen
DE19853185C1 (de) * 1998-11-19 2001-01-18 Guenter Von Kiedrowski Chemische Evolution durch exponentielle Amplifikation molekularer Matrizen
US6818395B1 (en) 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
WO2001029248A2 (en) * 1999-10-19 2001-04-26 Bionex, Inc. Method for amplifying and detecting nucleic acid
FR2802550B1 (fr) * 1999-12-17 2004-03-05 Centre Nat Rech Scient Biopuces, preparation et utilisations
EP1244812A1 (de) * 1999-12-21 2002-10-02 LION Bioscience AG Verzweigte verbindungen zur verwendung in reaktionen zum nachweis und zur analyse von nukleinsäuren
EP1111068A1 (de) * 1999-12-21 2001-06-27 LION Bioscience AG Verzweigte Substanz zur Verwendung in Reaktionen zur Detektion und Analyse von Nukleinsäuren
EP1130121A3 (de) * 2000-01-26 2003-04-16 Nisshinbo Industries Inc. Immobilisierte Nukleinsäuren und Methoden zum Nchhweis von Nukleinsäuren
JP2004523243A (ja) * 2001-03-12 2004-08-05 カリフォルニア インスティチュート オブ テクノロジー 非同期性塩基伸長によってポリヌクレオチド配列を分析するための方法および装置
DE10224339A1 (de) * 2002-05-29 2003-12-11 Axaron Bioscience Ag Verfahren zur hochparallelen Nukleinsäuresequenzierung
CA2513899C (en) 2003-01-29 2013-03-26 454 Corporation Methods of amplifying and sequencing nucleic acids
CN1761748A (zh) * 2003-03-18 2006-04-19 日本电气株式会社 Dna片段扩增方法,扩增dna片段的反应装置和制备该反应装置的方法
US8048627B2 (en) * 2003-07-05 2011-11-01 The Johns Hopkins University Method and compositions for detection and enumeration of genetic variations
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
KR101077603B1 (ko) * 2004-01-28 2011-10-27 삼성전자주식회사 카르복실기 또는 아미노기로 코팅된 고체상 물질을 이용한핵산의 증폭 방법
WO2005080605A2 (en) 2004-02-19 2005-09-01 Helicos Biosciences Corporation Methods and kits for analyzing polynucleotide sequences
US7635562B2 (en) 2004-05-25 2009-12-22 Helicos Biosciences Corporation Methods and devices for nucleic acid sequence determination
US7476734B2 (en) 2005-12-06 2009-01-13 Helicos Biosciences Corporation Nucleotide analogs
US7220549B2 (en) 2004-12-30 2007-05-22 Helicos Biosciences Corporation Stabilizing a nucleic acid for nucleic acid sequencing
US7482120B2 (en) 2005-01-28 2009-01-27 Helicos Biosciences Corporation Methods and compositions for improving fidelity in a nucleic acid synthesis reaction
EP3257949A1 (de) 2005-06-15 2017-12-20 Complete Genomics Inc. Nukleinsäureanalyse durch zufällige mischungen von nichtüberlappenden fragmenten
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
US7960104B2 (en) * 2005-10-07 2011-06-14 Callida Genomics, Inc. Self-assembled single molecule arrays and uses thereof
GB0522310D0 (en) 2005-11-01 2005-12-07 Solexa Ltd Methods of preparing libraries of template polynucleotides
CN101432439B (zh) * 2006-02-24 2013-07-24 考利达基因组股份有限公司 Dna阵列上的高通量基因组测序
SG170028A1 (en) * 2006-02-24 2011-04-29 Callida Genomics Inc High throughput genome sequencing on dna arrays
EP3617321B1 (de) 2006-05-31 2024-10-23 Sequenom, Inc. Kit zur nukleinsäureextraktion und -verstärkung aus einer probe
US7910354B2 (en) 2006-10-27 2011-03-22 Complete Genomics, Inc. Efficient arrays of amplified polynucleotides
US20080221832A1 (en) * 2006-11-09 2008-09-11 Complete Genomics, Inc. Methods for computing positional base probabilities using experminentals base value distributions
US20090111706A1 (en) 2006-11-09 2009-04-30 Complete Genomics, Inc. Selection of dna adaptor orientation by amplification
ES2665690T3 (es) 2007-02-02 2018-04-26 Genera Biosystems Limited Generación de moléculas de ácido nucleico
GB0712882D0 (en) * 2007-07-03 2007-08-15 Leicester University Of Nucleic acid amplification
US20090093378A1 (en) * 2007-08-29 2009-04-09 Helen Bignell Method for sequencing a polynucleotide template
US8518640B2 (en) * 2007-10-29 2013-08-27 Complete Genomics, Inc. Nucleic acid sequencing and process
US8415099B2 (en) 2007-11-05 2013-04-09 Complete Genomics, Inc. Efficient base determination in sequencing reactions
US8298768B2 (en) * 2007-11-29 2012-10-30 Complete Genomics, Inc. Efficient shotgun sequencing methods
US7897344B2 (en) * 2007-11-06 2011-03-01 Complete Genomics, Inc. Methods and oligonucleotide designs for insertion of multiple adaptors into library constructs
WO2009061840A1 (en) * 2007-11-05 2009-05-14 Complete Genomics, Inc. Methods and oligonucleotide designs for insertion of multiple adaptors employing selective methylation
US8592150B2 (en) 2007-12-05 2013-11-26 Complete Genomics, Inc. Methods and compositions for long fragment read sequencing
WO2009094583A1 (en) * 2008-01-23 2009-07-30 Complete Genomics, Inc. Methods and compositions for preventing bias in amplification and sequencing reactions
WO2009097368A2 (en) * 2008-01-28 2009-08-06 Complete Genomics, Inc. Methods and compositions for efficient base calling in sequencing reactions
WO2009132028A1 (en) * 2008-04-21 2009-10-29 Complete Genomics, Inc. Array structures for nucleic acid detection
JP5438922B2 (ja) * 2008-06-25 2014-03-12 日東電工株式会社 核酸の製造方法
DE102009012169B3 (de) 2009-03-06 2010-11-04 Albert-Ludwigs-Universität Freiburg Vorrichtung und Verfahren zum Herstellen eines Replikats oder eines Derivats aus einem Array von Molekülen und Anwendungen derselben
EP3514244B1 (de) 2009-04-03 2021-07-07 Sequenom, Inc. Verfahren für die herstellung von nukleinsäuren
US9524369B2 (en) 2009-06-15 2016-12-20 Complete Genomics, Inc. Processing and analysis of complex nucleic acid sequence data
KR20120045909A (ko) * 2010-11-01 2012-05-09 엘지전자 주식회사 복수 종의 표적 핵산을 실시간으로 검출하는 장치 및 방법
US20120152743A1 (en) * 2010-12-17 2012-06-21 General Electric Company Method for electroeluting genetic material from dried samples
DE102011054101A1 (de) 2011-09-30 2013-04-04 Albert-Ludwigs-Universität Freiburg Verfahren zur räumlichen Anordnung von Probenfragmenten zur Amplifikation und Immobilisierung für weitere Derivatisierungen
US10167501B2 (en) * 2014-06-12 2019-01-01 Auckland Uniservices Limited Methods and apparatus for quantification of nucleic acid amplification by monitoring impedances
US10955332B2 (en) 2016-12-22 2021-03-23 Illumina, Inc. Flow cell package and method for making the same
TW202120693A (zh) * 2019-08-21 2021-06-01 美商伊路米納有限公司 偵測核苷酸之聚合酶併入的方法

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4849077A (en) * 1984-08-06 1989-07-18 Akademie Der Wissenschaften Der Ddr Process for solid phase-sequencing of nucleic acid fragments
WO1990001546A1 (en) * 1988-08-05 1990-02-22 Applied Biotechnology, Inc. Equine herpesvirus-1 vaccine
WO1991000868A1 (en) * 1989-07-07 1991-01-24 National Research Development Corporation Immobilised polynucleotides
ATE152777T1 (de) * 1989-12-04 1997-05-15 Microprobe Corp Verstärkter einfang von ziel-nukleinsäuren mittels verwendung von oligonukleotiden, die kovalent an polymere gebunden sind
JP3011987B2 (ja) 1990-10-11 2000-02-21 旭化成工業株式会社 固相化プライマーを用いる逆転写酵素の測定法
US5437976A (en) * 1991-08-08 1995-08-01 Arizona Board Of Regents, The University Of Arizona Multi-domain DNA ligands bound to a solid matrix for protein and nucleic acid affinity chromatography and processing of solid-phase DNA
WO1993004199A2 (en) * 1991-08-20 1993-03-04 Scientific Generics Limited Methods of detecting or quantitating nucleic acids and of producing labelled immobilised nucleic acids
BR9206705A (pt) * 1991-11-01 1995-11-21 Adelaide Children S Hospital Processo de amplificação de fase sólida
FI943054A7 (fi) * 1991-12-24 1994-08-22 Tepnel Medical Ltd Nukleiinihapposekvenssien manipulointi
WO1993015228A1 (en) * 1992-01-29 1993-08-05 Hitachi Chemical Co., Ltd. Polynucleotide immobilized support
US5344701A (en) * 1992-06-09 1994-09-06 Minnesota Mining And Manufacturing Company Porous supports having azlactone-functional surfaces
US6060288A (en) * 1994-08-03 2000-05-09 Mosaic Technologies Method for performing amplification of nucleic acid on supports

Also Published As

Publication number Publication date
US6274351B1 (en) 2001-08-14
JP4067057B2 (ja) 2008-03-26
EP0787205A1 (de) 1997-08-06
DK0787205T3 (da) 1999-05-17
US6277604B1 (en) 2001-08-21
FR2726286A1 (fr) 1996-05-03
DE69504094T2 (de) 1999-03-11
EP0787205B1 (de) 1998-08-12
JPH10507923A (ja) 1998-08-04
DE69504094D1 (de) 1998-09-17
WO1996013609A1 (fr) 1996-05-09
FR2726286B1 (fr) 1997-01-17
ES2122692T3 (es) 1998-12-16

Similar Documents

Publication Publication Date Title
ATE169683T1 (de) Verfahren zur amplifikation von nukleinsäuren auf einer festphase und reagenziensatz dafür
ATE414171T1 (de) Verfahren zur immobilisierung von oligonukleotiden auf festem trägermaterial
ATE311474T1 (de) Verfahren zur amplifikation und zur sequenzierung von nukleinsäuren
DE69122712D1 (de) Verfahren zur nukleinsäure hybridisierung und amplifizierung
FI971611L (fi) DNA-polymeraaseja joilla on modifioitu nukleotidisitoutumiskohta DNA-sekvensointia varten
DE69607830D1 (de) Verfahren zur isothermer amplifikation von nukleinsäure molekülen
FI954331L (fi) Menetelmiä nukleiinihappojen sitomiseksi ja vapauttamiseksi selektiivisesti heikosti emäksistä polymeeriä käyttäen, sekä näiden monistamiseksi
NO20012735D0 (no) DNA-proteinfusjoner og anvendelser derav
DE59605824D1 (de) Verfahren zur transkriptionsfreien amplifizierung von nukleinsäuren
DE69706430D1 (de) Verfahren zur sequenzierung von dna
DE68928853D1 (de) Befestigung von sequenzspezifischen proben
DE58908694D1 (de) Oligonucleotidbank und verfahren zur dna-sequenzierung.
DE69734263D1 (de) Verfahren zur Isolierung von Ribonucleinsäuren.
ATE210192T1 (de) VERFAHREN ZUR DIREKTEN SEQUENZIERUNG WÄHREND ßTEMPLATEß-AMPLIFIKATION
ATE204290T1 (de) Zusammensetzungen und verfahren zur immobilisierung von nukleinsäure auf festträgern
DE69834129D1 (de) Verfahren und system zum vorausladen von information
DE69710077D1 (de) Verfahren zur erkennung von verbindungen, die die interaktion von rna und rna-bindeproteinen beeinflussen
ATE208827T1 (de) Konjugate von dns und pns und verfahren zu deren herstellung
DE69715006D1 (de) Verfahren zur sequenzierung von nukleinsäuren
ATE214429T1 (de) Verfahren zur amplifizierung spezifischer nukleinsäuresequenzen
DE69334048D1 (de) Verfahren fur nuklein saure hibridisierung mit verwendung von einzelstrangige dna bindende proteine
DE60030811D1 (de) Verfahren zur Ampifizierung von RNA
DE69637579D1 (de) Methode zur Amplifizierung von Nukleinsäure und entsprechende Regenzien dafür
DE69823372T8 (de) Verfahren und reagentiensatz zur amplifizierung, sequenzierung und typisierung von hla i genen
ATE212382T1 (de) Verfahren zur sequenzierung durch oligomerhybridisierung