ATE174968T1 - Verfahren mit einem gen-sonden biosensor - Google Patents

Verfahren mit einem gen-sonden biosensor

Info

Publication number
ATE174968T1
ATE174968T1 AT92919549T AT92919549T ATE174968T1 AT E174968 T1 ATE174968 T1 AT E174968T1 AT 92919549 T AT92919549 T AT 92919549T AT 92919549 T AT92919549 T AT 92919549T AT E174968 T1 ATE174968 T1 AT E174968T1
Authority
AT
Austria
Prior art keywords
immobilised
waveguide
wave detector
rna
hybridisation
Prior art date
Application number
AT92919549T
Other languages
English (en)
Inventor
David James Squirrell
Original Assignee
Secr Defence Brit
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Secr Defence Brit filed Critical Secr Defence Brit
Application granted granted Critical
Publication of ATE174968T1 publication Critical patent/ATE174968T1/de

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/648—Specially adapted constructive features of fluorimeters using evanescent coupling or surface plasmon coupling for the excitation of fluorescence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
    • G01N21/7703—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator using reagent-clad optical fibres or optical waveguides
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/81—Packaged device or kit

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Pathology (AREA)
  • Wood Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plasma & Fusion (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Steroid Compounds (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
AT92919549T 1991-09-16 1992-09-16 Verfahren mit einem gen-sonden biosensor ATE174968T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB919119735A GB9119735D0 (en) 1991-09-16 1991-09-16 Gene probe biosensor method

Publications (1)

Publication Number Publication Date
ATE174968T1 true ATE174968T1 (de) 1999-01-15

Family

ID=10701461

Family Applications (1)

Application Number Title Priority Date Filing Date
AT92919549T ATE174968T1 (de) 1991-09-16 1992-09-16 Verfahren mit einem gen-sonden biosensor

Country Status (20)

Country Link
US (1) US5750337A (de)
EP (1) EP0604506B1 (de)
JP (1) JP3324607B2 (de)
KR (1) KR100249288B1 (de)
AT (1) ATE174968T1 (de)
AU (1) AU665369B2 (de)
BG (1) BG62337B1 (de)
CA (1) CA2116239C (de)
CZ (1) CZ286927B6 (de)
DE (1) DE69227997T2 (de)
DK (1) DK0604506T3 (de)
ES (1) ES2125272T3 (de)
FI (1) FI108463B (de)
GB (2) GB9119735D0 (de)
GR (1) GR3029722T3 (de)
HU (1) HU218596B (de)
NO (1) NO315380B1 (de)
RU (1) RU2116349C1 (de)
SK (1) SK280713B6 (de)
WO (1) WO1993006241A1 (de)

Families Citing this family (42)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2133643A1 (en) * 1992-04-06 1993-10-14 Stanley R. Bouma Method and device for detection of nucleic acid or analyte using total internal reflectance
WO1995026416A1 (en) * 1994-03-25 1995-10-05 Research Corporation Technologies, Inc. Nucleic acid biosensor diagnostics
US5599668A (en) * 1994-09-22 1997-02-04 Abbott Laboratories Light scattering optical waveguide method for detecting specific binding events
WO1996019587A2 (en) * 1994-12-22 1996-06-27 Abbott Laboratories Methods of immobilizing oligonucleotides to solid support materials and methods of using support bound oligonucleotides
US5690894A (en) * 1995-05-23 1997-11-25 The Regents Of The University Of California High density array fabrication and readout method for a fiber optic biosensor
SE9502608D0 (sv) * 1995-07-14 1995-07-14 Pharmacia Biosensor Ab Method for nucleic acid senquencing
DE69733650T2 (de) * 1996-03-01 2006-04-20 Beckman Coulter, Inc., Fullerton System zum simultanen Durchführen einer Vielzahl von Ligandenbindungs-Untersuchungen
US5832165A (en) * 1996-08-28 1998-11-03 University Of Utah Research Foundation Composite waveguide for solid phase binding assays
NZ502303A (en) 1997-06-18 2002-02-01 Ulrich J Krull Nucleic acid biosensor system & diagnostic use
DE19730359A1 (de) * 1997-07-15 1999-01-21 Boehringer Mannheim Gmbh Integriertes Verfahren und System zur Amplifizierung und zum Nachweis von Nukleinsäuren
JP2001511358A (ja) * 1997-07-28 2001-08-14 メディカル・バイオシステムズ・リミテッド 核酸配列の解析
GB2335035B (en) * 1998-03-03 2003-05-28 Brax Genomics Ltd Screening for functional antisense agents
GB9805935D0 (en) * 1998-03-19 1998-05-13 Ciba Geigy Ag Organic compounds
EP0957354A3 (de) * 1998-03-20 2000-03-22 IA Inc. Verfahren und Vorrichtung zum Messen der Bindung zwischen Biomolekülen
US6429023B1 (en) 1998-07-20 2002-08-06 Shayda Technologies, Inc. Biosensors with polymeric optical waveguides
EP1046717B1 (de) * 1999-04-20 2010-10-06 National Institute of Advanced Industrial Science and Technology Verfahren und Sonden zur Bestimmung der Konzentration von Nukleinsäure-Molekülen und Verfahren zur Analyse der gewonnenen Daten
RU2182708C2 (ru) * 2000-04-17 2002-05-20 Институт молекулярной биологии им. В.А. Энгельгардта РАН Способ множественного параллельного скрининга специфичности связывания биологически активных соединений с нуклеиновыми кислотами с использованием биочипа (варианты)
WO2002033005A2 (en) * 2000-10-19 2002-04-25 Trans Photonics, L.L.C. Novel substituted-polyaryl chromophoric compounds
AR031640A1 (es) * 2000-12-08 2003-09-24 Applied Research Systems Amplificacion isotermica de acidos nucleicos en un soporte solido
US6597499B2 (en) 2001-01-25 2003-07-22 Olympus Optical Co., Ltd. Total internal reflection fluorescence microscope having a conventional white-light source
RU2194761C2 (ru) * 2001-03-15 2002-12-20 Гематологический научный центр РАМН Способ конструирования внутреннего стандарта для количественного определения днк методом конкурентной полимеразной цепной реакции
WO2003029435A2 (en) * 2001-10-04 2003-04-10 The Regents Of The University Of California Detection of polynucleotide hybridization
RU2206615C1 (ru) * 2001-10-22 2003-06-20 Институт молекулярной биологии им. В.А. Энгельгардта РАН Способ частичного секвенирования днк для определения мутаций в коротких фрагментах одноцепочечной днк с использованием микрочипа
DE102004016247A1 (de) * 2004-04-02 2005-10-20 Chromeon Gmbh Feste Trägersysteme zum homogenen Nachweis von Wechselwirkungen von Biomolekülen ohne Waschschritte
RU2270254C2 (ru) * 2004-04-30 2006-02-20 Институт Молекулярной Биологии Им. В.А. Энгельгардта Российской Академии Наук Способ идентификации трансгенных последовательностей днк в растительном материале и продуктах на его основе, набор олигонуклеотидов и биочип для осуществления этого способа
US20060088844A1 (en) * 2004-10-22 2006-04-27 Honeywell International Inc. Real-time PCR microarray based on evanescent wave biosensor
RU2315999C2 (ru) * 2004-11-24 2008-01-27 Александр Иванович Арчаков Нанодиагностическая тест-система для выявления вируса гепатитов
GB0522310D0 (en) 2005-11-01 2005-12-07 Solexa Ltd Methods of preparing libraries of template polynucleotides
GB0524069D0 (en) * 2005-11-25 2006-01-04 Solexa Ltd Preparation of templates for solid phase amplification
JP5429962B2 (ja) * 2006-03-16 2014-02-26 国立大学法人秋田大学 核酸検出方法及び核酸検出キット
WO2007107710A1 (en) * 2006-03-17 2007-09-27 Solexa Limited Isothermal methods for creating clonal single molecule arrays
US7824902B2 (en) * 2006-11-03 2010-11-02 Mark Selker Optical interface for disposable bioreactors
EP1935496A1 (de) 2006-12-22 2008-06-25 Eppendorf Array Technologies SA Vorrichtung und/oder Verfahren zur Detektion erweiterter Nukleotidsequenzen auf Mikro-Arrays
US8124944B2 (en) * 2007-01-17 2012-02-28 Honeywell International Inc. Microarray reader based on evanescent wave detection and method of reading a microarray
WO2008093098A2 (en) 2007-02-02 2008-08-07 Illumina Cambridge Limited Methods for indexing samples and sequencing multiple nucleotide templates
WO2009079857A1 (en) * 2007-12-24 2009-07-02 Honeywell International Inc. A reactor for the quantitative analysis of nucleic acids
EP2231876B1 (de) * 2007-12-24 2012-08-01 Honeywell International Inc. Programmierbarer oligonukleotid-mikroarray
WO2009098605A1 (en) 2008-02-04 2009-08-13 Koninklijke Philips Electronics N.V. Molecular diagnostic system based on evanescent illumination and fluorescence
CN102791882A (zh) 2010-01-20 2012-11-21 霍尼韦尔国际公司 用于核酸定量分析的反应器
US20140371082A1 (en) * 2011-06-30 2014-12-18 Bioquanta Sa Process and apparatus for quantifying nucleic acid in a sample
BR112019007865A2 (pt) * 2016-10-27 2019-07-02 Vanadis Diagnostics método para processamento de produtos de amplificação do círculo rolante
JP2022523799A (ja) * 2019-03-01 2022-04-26 ヴィディア・ホールディングス・リミテッド 光学要素における、または関連した改善

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1180647A (en) * 1981-07-17 1985-01-08 Cavit Akin Light-emitting polynucleotide hybridization diagnostic method
US4582809A (en) * 1982-06-14 1986-04-15 Myron J. Block Apparatus including optical fiber for fluorescence immunoassay
EP0144914A3 (de) * 1983-12-12 1986-08-13 Miles Inc. Hybridisierungstest mittels markierter Paare von hybridbindenden Reagenzien
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US5242797A (en) * 1986-03-21 1993-09-07 Myron J. Block Nucleic acid assay method
EP0245206A1 (de) * 1986-05-05 1987-11-11 IntraCel Corporation Analytisches Verfahren zum Nachweis und zur Messung einer spezifisch sequentierten Nukleinsäure
BE1000572A4 (fr) * 1987-05-20 1989-02-07 Block Myron J Cellule rta, appareil et procede pour titrer un polynucleotide dans un liquide.
CA1323293C (en) * 1987-12-11 1993-10-19 Keith C. Backman Assay using template-dependent nucleic acid probe reorganization
GB8902689D0 (en) * 1989-02-07 1989-03-30 Ici Plc Assay method
GB8910880D0 (en) * 1989-05-11 1989-06-28 Amersham Int Plc Sequencing method
GB8919411D0 (en) * 1989-08-25 1989-10-11 Amersham Int Plc Assay method
CA2032203C (en) * 1989-12-29 2009-05-19 Christine L. Brakel Amplification capture assay
EP0478319B1 (de) * 1990-09-28 1997-04-02 Kabushiki Kaisha Toshiba Verfahren zum Gennachweis
USH1398H (en) * 1990-12-28 1995-01-03 Campbell; James R. DNA-based fluourescent sensor

Also Published As

Publication number Publication date
GB2274710A (en) 1994-08-03
CA2116239A1 (en) 1993-04-01
JP3324607B2 (ja) 2002-09-17
DE69227997D1 (de) 1999-02-04
FI941220A0 (fi) 1994-03-15
BG62337B1 (bg) 1999-08-31
NO940901D0 (no) 1994-03-14
US5750337A (en) 1998-05-12
AU665369B2 (en) 1996-01-04
CA2116239C (en) 2008-11-18
WO1993006241A1 (en) 1993-04-01
NO940901L (no) 1994-05-16
HU9400770D0 (en) 1994-06-28
AU2564192A (en) 1993-04-27
BG98660A (bg) 1995-03-31
EP0604506A1 (de) 1994-07-06
RU94019336A (ru) 1996-09-27
HUT68771A (en) 1995-07-28
NO315380B1 (no) 2003-08-25
KR100249288B1 (ko) 2000-04-01
EP0604506B1 (de) 1998-12-23
SK280713B6 (sk) 2000-06-12
SK30694A3 (en) 1994-07-06
RU2116349C1 (ru) 1998-07-27
FI108463B (fi) 2002-01-31
DE69227997T2 (de) 1999-05-12
GR3029722T3 (en) 1999-06-30
CZ52194A3 (en) 1994-07-13
DK0604506T3 (da) 1999-08-23
ES2125272T3 (es) 1999-03-01
GB9119735D0 (en) 1991-10-30
HU218596B (hu) 2000-10-28
JPH06510670A (ja) 1994-12-01
CZ286927B6 (cs) 2000-08-16
FI941220L (fi) 1994-03-15
GB2274710B (en) 1995-09-06
GB9402823D0 (en) 1994-04-27

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Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification
REN Ceased due to non-payment of the annual fee