ES2125272T3 - Metodo de biosensor de sonda genica. - Google Patents

Metodo de biosensor de sonda genica.

Info

Publication number
ES2125272T3
ES2125272T3 ES92919549T ES92919549T ES2125272T3 ES 2125272 T3 ES2125272 T3 ES 2125272T3 ES 92919549 T ES92919549 T ES 92919549T ES 92919549 T ES92919549 T ES 92919549T ES 2125272 T3 ES2125272 T3 ES 2125272T3
Authority
ES
Spain
Prior art keywords
genic
rna
biosensor method
analysis
probe biosensor
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES92919549T
Other languages
English (en)
Inventor
David James Squirrell
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
UK Secretary of State for Defence
Original Assignee
UK Secretary of State for Defence
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by UK Secretary of State for Defence filed Critical UK Secretary of State for Defence
Application granted granted Critical
Publication of ES2125272T3 publication Critical patent/ES2125272T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/648—Specially adapted constructive features of fluorimeters using evanescent coupling or surface plasmon coupling for the excitation of fluorescence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
    • G01N21/7703—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator using reagent-clad optical fibres or optical waveguides
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/81—Packaged device or kit

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Pathology (AREA)
  • Wood Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Plasma & Fusion (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Steroid Compounds (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

LAS PRESENTE INVENCION SE REFIERE A UN METODO PARA LA DETECCION, IDENTIFICACION Y/O CUANTIFICACION DE TEJIDOS ANIMALES O VEGETALES, MICROORGANISMOS O RNA O ADN LIBRE DE CELULAS Y A APARATO DETECTOR ADAPTADO PARA REALIZAR DICHO METODO. EL METODO USA PARTICULARMENTE FLUORESCENCIA DE REFLEXION INTERNA TOTAL (TIRF) PARA MEDIR LA HIBRIDACION DE RNA O ADN DE PRODUCTO DE ANALISIS CON RNA O ADN QUE ESTA ASOCIADO CON UN GUIAONDAS DETECTOR DE ONDAS EVANESCENTES.
ES92919549T 1991-09-16 1992-09-16 Metodo de biosensor de sonda genica. Expired - Lifetime ES2125272T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB919119735A GB9119735D0 (en) 1991-09-16 1991-09-16 Gene probe biosensor method

Publications (1)

Publication Number Publication Date
ES2125272T3 true ES2125272T3 (es) 1999-03-01

Family

ID=10701461

Family Applications (1)

Application Number Title Priority Date Filing Date
ES92919549T Expired - Lifetime ES2125272T3 (es) 1991-09-16 1992-09-16 Metodo de biosensor de sonda genica.

Country Status (20)

Country Link
US (1) US5750337A (es)
EP (1) EP0604506B1 (es)
JP (1) JP3324607B2 (es)
KR (1) KR100249288B1 (es)
AT (1) ATE174968T1 (es)
AU (1) AU665369B2 (es)
BG (1) BG62337B1 (es)
CA (1) CA2116239C (es)
CZ (1) CZ286927B6 (es)
DE (1) DE69227997T2 (es)
DK (1) DK0604506T3 (es)
ES (1) ES2125272T3 (es)
FI (1) FI108463B (es)
GB (2) GB9119735D0 (es)
GR (1) GR3029722T3 (es)
HU (1) HU218596B (es)
NO (1) NO315380B1 (es)
RU (1) RU2116349C1 (es)
SK (1) SK280713B6 (es)
WO (1) WO1993006241A1 (es)

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WO1993020240A1 (en) * 1992-04-06 1993-10-14 Abbott Laboratories Method and device for detection of nucleic acid or analyte using total internal reflectance
WO1995026416A1 (en) * 1994-03-25 1995-10-05 Research Corporation Technologies, Inc. Nucleic acid biosensor diagnostics
US5599668A (en) * 1994-09-22 1997-02-04 Abbott Laboratories Light scattering optical waveguide method for detecting specific binding events
EP0795032A2 (en) * 1994-12-22 1997-09-17 Abbott Laboratories Methods of immobilizing oligonucleotides to solid support materials and methods of using support bound oligonucleotides
US5690894A (en) 1995-05-23 1997-11-25 The Regents Of The University Of California High density array fabrication and readout method for a fiber optic biosensor
SE9502608D0 (sv) * 1995-07-14 1995-07-14 Pharmacia Biosensor Ab Method for nucleic acid senquencing
WO1997032212A1 (en) * 1996-03-01 1997-09-04 Beckman Instruments, Inc. System for simultaneously conducting multiple ligand binding assays
US5832165A (en) * 1996-08-28 1998-11-03 University Of Utah Research Foundation Composite waveguide for solid phase binding assays
AU755913B2 (en) 1997-06-18 2003-01-02 Masad Damha Nucleic acid biosensor diagnostics
DE19730359A1 (de) * 1997-07-15 1999-01-21 Boehringer Mannheim Gmbh Integriertes Verfahren und System zur Amplifizierung und zum Nachweis von Nukleinsäuren
DK1017848T3 (da) * 1997-07-28 2003-02-10 Medical Biosystems Ltd Nucleinsyresekvensanalyse
GB2335035B (en) * 1998-03-03 2003-05-28 Brax Genomics Ltd Screening for functional antisense agents
GB9805935D0 (en) * 1998-03-19 1998-05-13 Ciba Geigy Ag Organic compounds
EP0957354A3 (en) * 1998-03-20 2000-03-22 IA Inc. Method and apparatus for measuring binding between biological molecules
US6429023B1 (en) 1998-07-20 2002-08-06 Shayda Technologies, Inc. Biosensors with polymeric optical waveguides
DE60045059D1 (de) * 1999-04-20 2010-11-18 Nat Inst Of Advanced Ind Scien Verfahren und Sonden zur Bestimmung der Konzentration von Nukleinsäure-Molekülen und Verfahren zur Analyse der gewonnenen Daten
RU2182708C2 (ru) * 2000-04-17 2002-05-20 Институт молекулярной биологии им. В.А. Энгельгардта РАН Способ множественного параллельного скрининга специфичности связывания биологически активных соединений с нуклеиновыми кислотами с использованием биочипа (варианты)
US7205347B2 (en) * 2000-10-19 2007-04-17 Trans Photonics, Llc. Substituted-polyaryl chromophoric compounds
AR031640A1 (es) * 2000-12-08 2003-09-24 Applied Research Systems Amplificacion isotermica de acidos nucleicos en un soporte solido
US6597499B2 (en) 2001-01-25 2003-07-22 Olympus Optical Co., Ltd. Total internal reflection fluorescence microscope having a conventional white-light source
RU2194761C2 (ru) * 2001-03-15 2002-12-20 Гематологический научный центр РАМН Способ конструирования внутреннего стандарта для количественного определения днк методом конкурентной полимеразной цепной реакции
US20040241699A1 (en) * 2001-10-04 2004-12-02 Giovanni Zocchi Detection of polynucleotide hybridization
RU2206615C1 (ru) * 2001-10-22 2003-06-20 Институт молекулярной биологии им. В.А. Энгельгардта РАН Способ частичного секвенирования днк для определения мутаций в коротких фрагментах одноцепочечной днк с использованием микрочипа
DE102004016247A1 (de) * 2004-04-02 2005-10-20 Chromeon Gmbh Feste Trägersysteme zum homogenen Nachweis von Wechselwirkungen von Biomolekülen ohne Waschschritte
RU2270254C2 (ru) * 2004-04-30 2006-02-20 Институт Молекулярной Биологии Им. В.А. Энгельгардта Российской Академии Наук Способ идентификации трансгенных последовательностей днк в растительном материале и продуктах на его основе, набор олигонуклеотидов и биочип для осуществления этого способа
US20060088844A1 (en) * 2004-10-22 2006-04-27 Honeywell International Inc. Real-time PCR microarray based on evanescent wave biosensor
RU2315999C2 (ru) * 2004-11-24 2008-01-27 Александр Иванович Арчаков Нанодиагностическая тест-система для выявления вируса гепатитов
GB0522310D0 (en) * 2005-11-01 2005-12-07 Solexa Ltd Methods of preparing libraries of template polynucleotides
GB0524069D0 (en) * 2005-11-25 2006-01-04 Solexa Ltd Preparation of templates for solid phase amplification
JP5429962B2 (ja) * 2006-03-16 2014-02-26 国立大学法人秋田大学 核酸検出方法及び核酸検出キット
EP2021503A1 (en) * 2006-03-17 2009-02-11 Solexa Ltd. Isothermal methods for creating clonal single molecule arrays
US7824902B2 (en) * 2006-11-03 2010-11-02 Mark Selker Optical interface for disposable bioreactors
EP1935496A1 (en) 2006-12-22 2008-06-25 Eppendorf Array Technologies SA Device and/or method for the detection of amplified nucleotides sequences on micro-arrays
WO2008092291A1 (en) * 2007-01-17 2008-08-07 Honeywell International Inc. Microarray reader based on evanescent wave detection and method of reading a microarray
WO2008093098A2 (en) 2007-02-02 2008-08-07 Illumina Cambridge Limited Methods for indexing samples and sequencing multiple nucleotide templates
WO2009079856A1 (en) * 2007-12-24 2009-07-02 Honeywell International Inc. A programmable oligonucleotme micro array
US9480982B2 (en) * 2007-12-24 2016-11-01 Honeywell International Inc. Reactor for the quantitative analysis of nucleic acids
JP5542067B2 (ja) 2008-02-04 2014-07-09 コーニンクレッカ フィリップス エヌ ヴェ エバネセント照射及び蛍光に基づく分子診断システム
WO2011088588A1 (en) 2010-01-20 2011-07-28 Honeywell International, Inc. Reactor for quantitative analysis of nucleic acids
WO2013003849A2 (en) * 2011-06-30 2013-01-03 Bioquanta Sa Process and apparatus for quantifying nucleic acid in a sample
JP6997775B2 (ja) * 2016-10-27 2022-01-18 バナディス ダイアグノスティクス ローリングサークル増幅産物を処理する方法
EP3930576A1 (en) * 2019-03-01 2022-01-05 Vidya Holdings Ltd Improvements in or relating to an optical element

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CA1180647A (en) * 1981-07-17 1985-01-08 Cavit Akin Light-emitting polynucleotide hybridization diagnostic method
US4582809A (en) * 1982-06-14 1986-04-15 Myron J. Block Apparatus including optical fiber for fluorescence immunoassay
EP0144914A3 (en) * 1983-12-12 1986-08-13 Miles Inc. Hybridization assay employing labeled pairs of hybrid binding reagents
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US5242797A (en) * 1986-03-21 1993-09-07 Myron J. Block Nucleic acid assay method
EP0245206A1 (en) * 1986-05-05 1987-11-11 IntraCel Corporation Analytical method for detecting and measuring specifically sequenced nucleic acid
BE1000572A4 (fr) * 1987-05-20 1989-02-07 Block Myron J Cellule rta, appareil et procede pour titrer un polynucleotide dans un liquide.
AU622426B2 (en) * 1987-12-11 1992-04-09 Abbott Laboratories Assay using template-dependent nucleic acid probe reorganization
GB8902689D0 (en) * 1989-02-07 1989-03-30 Ici Plc Assay method
GB8910880D0 (en) * 1989-05-11 1989-06-28 Amersham Int Plc Sequencing method
GB8919411D0 (en) * 1989-08-25 1989-10-11 Amersham Int Plc Assay method
CA2032203C (en) * 1989-12-29 2009-05-19 Christine L. Brakel Amplification capture assay
EP0478319B1 (en) * 1990-09-28 1997-04-02 Kabushiki Kaisha Toshiba Gene detection method
USH1398H (en) * 1990-12-28 1995-01-03 Campbell; James R. DNA-based fluourescent sensor

Also Published As

Publication number Publication date
CZ52194A3 (en) 1994-07-13
HU218596B (hu) 2000-10-28
AU665369B2 (en) 1996-01-04
NO315380B1 (no) 2003-08-25
BG62337B1 (bg) 1999-08-31
ATE174968T1 (de) 1999-01-15
FI108463B (fi) 2002-01-31
JP3324607B2 (ja) 2002-09-17
EP0604506B1 (en) 1998-12-23
HU9400770D0 (en) 1994-06-28
AU2564192A (en) 1993-04-27
KR100249288B1 (ko) 2000-04-01
DE69227997D1 (de) 1999-02-04
GB2274710A (en) 1994-08-03
RU94019336A (ru) 1996-09-27
WO1993006241A1 (en) 1993-04-01
CA2116239A1 (en) 1993-04-01
GB9119735D0 (en) 1991-10-30
DE69227997T2 (de) 1999-05-12
GR3029722T3 (en) 1999-06-30
GB2274710B (en) 1995-09-06
CA2116239C (en) 2008-11-18
HUT68771A (en) 1995-07-28
CZ286927B6 (cs) 2000-08-16
JPH06510670A (ja) 1994-12-01
US5750337A (en) 1998-05-12
NO940901L (no) 1994-05-16
SK30694A3 (en) 1994-07-06
BG98660A (bg) 1995-03-31
SK280713B6 (sk) 2000-06-12
EP0604506A1 (en) 1994-07-06
GB9402823D0 (en) 1994-04-27
FI941220A0 (fi) 1994-03-15
RU2116349C1 (ru) 1998-07-27
FI941220L (fi) 1994-03-15
DK0604506T3 (da) 1999-08-23
NO940901D0 (no) 1994-03-14

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