ATE241017T1 - Nukleinsäure - sandwichassay in lösungsphase mit reduzierten hintergrundsignalen - Google Patents

Nukleinsäure - sandwichassay in lösungsphase mit reduzierten hintergrundsignalen

Info

Publication number
ATE241017T1
ATE241017T1 AT95904290T AT95904290T ATE241017T1 AT E241017 T1 ATE241017 T1 AT E241017T1 AT 95904290 T AT95904290 T AT 95904290T AT 95904290 T AT95904290 T AT 95904290T AT E241017 T1 ATE241017 T1 AT E241017T1
Authority
AT
Austria
Prior art keywords
solution phase
background signals
nucleic acid
sandwich assay
reduced background
Prior art date
Application number
AT95904290T
Other languages
English (en)
Inventor
Michael S Urdea
Timothy Fultz
Brian D Warner
Mark Collins
Original Assignee
Bayer Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bayer Ag filed Critical Bayer Ag
Application granted granted Critical
Publication of ATE241017T1 publication Critical patent/ATE241017T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F02—COMBUSTION ENGINES; HOT-GAS OR COMBUSTION-PRODUCT ENGINE PLANTS
    • F02B—INTERNAL-COMBUSTION PISTON ENGINES; COMBUSTION ENGINES IN GENERAL
    • F02B75/00—Other engines
    • F02B75/02—Engines characterised by their cycles, e.g. six-stroke
    • F02B2075/022—Engines characterised by their cycles, e.g. six-stroke having less than six strokes per cycle
    • F02B2075/027—Engines characterised by their cycles, e.g. six-stroke having less than six strokes per cycle four
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/808—Optical sensing apparatus
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/81—Packaged device or kit

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
AT95904290T 1993-12-08 1994-12-07 Nukleinsäure - sandwichassay in lösungsphase mit reduzierten hintergrundsignalen ATE241017T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/164,388 US5681697A (en) 1993-12-08 1993-12-08 Solution phase nucleic acid sandwich assays having reduced background noise and kits therefor
PCT/US1994/014119 WO1995016055A1 (en) 1993-12-08 1994-12-07 Solution phase nucleic acid sandwich assays having reduced background noise

Publications (1)

Publication Number Publication Date
ATE241017T1 true ATE241017T1 (de) 2003-06-15

Family

ID=22594262

Family Applications (1)

Application Number Title Priority Date Filing Date
AT95904290T ATE241017T1 (de) 1993-12-08 1994-12-07 Nukleinsäure - sandwichassay in lösungsphase mit reduzierten hintergrundsignalen

Country Status (11)

Country Link
US (2) US5681697A (de)
EP (1) EP0731848B1 (de)
JP (4) JP3802925B2 (de)
KR (1) KR100327610B1 (de)
AT (1) ATE241017T1 (de)
AU (1) AU694468B2 (de)
CA (1) CA2178598A1 (de)
DE (1) DE69432709T2 (de)
ES (1) ES2199236T3 (de)
HU (1) HU221237B1 (de)
WO (1) WO1995016055A1 (de)

Families Citing this family (345)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7825215B1 (en) 1993-04-26 2010-11-02 Peter E. Nielsen Substituted nucleic acid mimics
US7582421B2 (en) * 1993-11-01 2009-09-01 Nanogen, Inc. Methods for determination of single nucleic acid polymorphisms using a bioelectronic microchip
US5681702A (en) * 1994-08-30 1997-10-28 Chiron Corporation Reduction of nonspecific hybridization by using novel base-pairing schemes
WO1996020287A2 (en) * 1994-12-23 1996-07-04 Behringwerke Aktiengesellschaft Detection of nucleic acids by target-catalyzed product formation
FR2737502B1 (fr) 1995-07-31 1997-10-24 Genset Sa Procede de detection d'acides nucleiques utilisant des sondes nucleotidiques permettant a la fois une capture specifique et une detection
DE19537952A1 (de) * 1995-10-12 1997-04-17 Boehringer Mannheim Gmbh Verfahren zum Nachweis eines Analyten
WO1997023647A1 (en) * 1995-12-22 1997-07-03 Behringwerke Aktiengesellschaft Homogeneous amplification and detection of nucleic acids
US6013439A (en) * 1995-12-22 2000-01-11 Dade Behring Marburg Gmbh Detection of differences in nucleic acids
US5824478A (en) * 1996-04-30 1998-10-20 Vysis, Inc. Diagnostic methods and probes
CA2259918A1 (en) 1996-07-12 1998-01-22 Tm Technologies, Inc. Signal amplification method
DK0975807T3 (da) 1997-05-02 2007-01-29 Gen Probe Inc To-trins hybridisering og indfangning af et polynukleotid
US6534273B2 (en) 1997-05-02 2003-03-18 Gen-Probe Incorporated Two-step hybridization and capture of a polynucleotide
US6406857B1 (en) 1997-06-16 2002-06-18 Exact Sciences Corporation Methods for stool sample preparation
US6268136B1 (en) 1997-06-16 2001-07-31 Exact Science Corporation Methods for stool sample preparation
US5939261A (en) * 1997-06-24 1999-08-17 Sarnoff Corporation Method for capturing a nucleic acid
PT1038022E (pt) * 1997-12-12 2005-11-30 Digene Corp Avaliacao de doencas relacionadas com o virus papiloma humano
US20070166741A1 (en) * 1998-12-14 2007-07-19 Somalogic, Incorporated Multiplexed analyses of test samples
US6242246B1 (en) * 1997-12-15 2001-06-05 Somalogic, Inc. Nucleic acid ligand diagnostic Biochip
US6451526B1 (en) 1998-01-20 2002-09-17 Mayo Foundation For Medical Education And Research Simplified mutation detection
US6289229B1 (en) 1998-01-20 2001-09-11 Scimed Life Systems, Inc. Readable probe array for in vivo use
US6365346B1 (en) 1998-02-18 2002-04-02 Dade Behring Inc. Quantitative determination of nucleic acid amplification products
GB9805935D0 (en) * 1998-03-19 1998-05-13 Ciba Geigy Ag Organic compounds
WO2000004192A1 (en) * 1998-07-17 2000-01-27 Emory University Methods for detecting and mapping genes, mutations and variant polynucleotide sequences
EP1271154A3 (de) 1998-09-18 2005-08-17 Massachusetts Institute Of Technology Biologische Verwendung von Halbleiter-Nanokristallen
US6203989B1 (en) 1998-09-30 2001-03-20 Affymetrix, Inc. Methods and compositions for amplifying detectable signals in specific binding assays
US20030032029A1 (en) * 1998-12-21 2003-02-13 Nanogen, Inc. Three dimensional apparatus and method for integrating sample preparation and multiplex assays
US6432642B1 (en) * 1999-01-15 2002-08-13 Pe Corporation (Ny) Binary probe and clamp composition and methods for a target hybridization detection
EP1155147B1 (de) 1999-02-25 2006-06-14 EXACT Sciences Corporation Verfahren zur erhaltung der dns-integrität
GB9905580D0 (en) * 1999-03-12 1999-05-05 Tepnel Medical Ltd Enyzymatically catalysed signal amplification
JP4794052B2 (ja) 1999-04-09 2011-10-12 ジェンザイム・コーポレーション 癌の指標である核酸を検出するための方法
US20060275782A1 (en) 1999-04-20 2006-12-07 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
DK1923471T3 (da) 1999-04-20 2013-04-02 Illumina Inc Detektion af nukleinsyrereaktioner på bead-arrays
US8080380B2 (en) 1999-05-21 2011-12-20 Illumina, Inc. Use of microfluidic systems in the detection of target analytes using microsphere arrays
US8481268B2 (en) 1999-05-21 2013-07-09 Illumina, Inc. Use of microfluidic systems in the detection of target analytes using microsphere arrays
US20020045182A1 (en) * 1999-07-16 2002-04-18 Lynx Therapeutics, Inc. Multiplexed differential displacement for nucleic acid determinations
US6849403B1 (en) 1999-09-08 2005-02-01 Exact Sciences Corporation Apparatus and method for drug screening
US6586177B1 (en) * 1999-09-08 2003-07-01 Exact Sciences Corporation Methods for disease detection
US6428957B1 (en) 1999-11-08 2002-08-06 Agilent Technologies, Inc. Systems tools and methods of assaying biological materials using spatially-addressable arrays
US7368233B2 (en) 1999-12-07 2008-05-06 Exact Sciences Corporation Methods of screening for lung neoplasm based on stool samples containing a nucleic acid marker indicative of a neoplasm
US6919174B1 (en) 1999-12-07 2005-07-19 Exact Sciences Corporation Methods for disease detection
US6235483B1 (en) 2000-01-31 2001-05-22 Agilent Technologies, Inc. Methods and kits for indirect labeling of nucleic acids
US7611869B2 (en) 2000-02-07 2009-11-03 Illumina, Inc. Multiplexed methylation detection methods
US8076063B2 (en) 2000-02-07 2011-12-13 Illumina, Inc. Multiplexed methylation detection methods
ATE411397T1 (de) 2000-02-07 2008-10-15 Illumina Inc Nukleinsäure-nachweisverfahren mit universellem priming
US7955794B2 (en) 2000-09-21 2011-06-07 Illumina, Inc. Multiplex nucleic acid reactions
US7582420B2 (en) 2001-07-12 2009-09-01 Illumina, Inc. Multiplex nucleic acid reactions
US20020094538A1 (en) * 2001-01-13 2002-07-18 Getts Robert C. Methods for detecting and assaying nucleic acid sequences using temperature cycling
WO2001073122A1 (en) * 2000-03-28 2001-10-04 Nanogen, Inc. Methods for determination of single nucleic acid polymorphisms using a bioelectronic microchip
AU2001249674A1 (en) * 2000-03-29 2001-10-08 Polyprobe, Inc. Quality control reagents for nucleic acid microarrays
US7439016B1 (en) * 2000-06-15 2008-10-21 Digene Corporation Detection of nucleic acids by type-specific hybrid capture method
US7601497B2 (en) * 2000-06-15 2009-10-13 Qiagen Gaithersburg, Inc. Detection of nucleic acids by target-specific hybrid capture method
GB0016813D0 (en) * 2000-07-07 2000-08-30 Lee Helen Improved dipstick assays (4)
GB0016836D0 (en) * 2000-07-07 2000-08-30 Lee Helen Improved dipstick assays (1)
GB0016833D0 (en) * 2000-07-07 2000-08-30 Lee Helen Improved dipstick assays (2)
GB0016814D0 (en) * 2000-07-07 2000-08-30 Lee Helen Improved dipstick assays (3)
EP2020450B9 (de) 2000-09-01 2011-09-14 Gen-Probe Incorporated HIV-1-Sequenzverstärkung zur Erkennung von Sequenzen in Zusammenhang mit Wirkstoffresistenz-Mutationen
US20030077590A1 (en) * 2000-09-22 2003-04-24 Pedersen Finn Skou Methods for diagnosis and treatment of diseases associated with altered expression of neurogranin
US20030044803A1 (en) * 2000-09-22 2003-03-06 Pedersen Finn Skou Methods for diagnosis and treatment of diseases associated with altered expression of JAK1
US20020115058A1 (en) * 2000-09-22 2002-08-22 Pedersen Finn Skou Methods for diagnosis and treatment of diseases associated with altered expression of Pik3r1
US6806051B2 (en) * 2000-09-25 2004-10-19 Picoliter Inc. Arrays of partially nonhybridizing oligonucleotides and preparation thereof using focused acoustic energy
WO2002031206A2 (en) * 2000-10-11 2002-04-18 Ragland William L Methods and compositions using hybridization assays for detecting infectious agents
US7700274B2 (en) * 2000-12-22 2010-04-20 Sagres Discovery, Inc. Compositions and methods in cancer associated with altered expression of KCNJ9
US7820447B2 (en) * 2000-12-22 2010-10-26 Sagres Discovery Inc. Compositions and methods for cancer
US20030232334A1 (en) * 2000-12-22 2003-12-18 Morris David W. Novel compositions and methods for cancer
US7645441B2 (en) 2000-12-22 2010-01-12 Sagres Discovery Inc. Compositions and methods in cancer associated with altered expression of PRLR
US7892730B2 (en) * 2000-12-22 2011-02-22 Sagres Discovery, Inc. Compositions and methods for cancer
US20030099963A1 (en) * 2000-12-22 2003-05-29 Morris David W. Novel compositions and methods in cancer associated with altered expression of TBX21
US20030087252A1 (en) * 2000-12-22 2003-05-08 Morris David W. Novel compositions and methods in cancer associated with altered expression of PRDM11
US20030165878A1 (en) * 2000-12-22 2003-09-04 Morris David W. Novel compositions and methods in cancer associated with altered expression of MCM3AP
GB0101397D0 (en) * 2001-01-19 2001-03-07 Amersham Pharm Biotech Uk Ltd Suppression of non-specific nucleic acid amplication
US20030092157A1 (en) * 2001-03-16 2003-05-15 Hayden Michael R. Compositions, screening systems and methods for modulating HDL cholesterol and triglyceride levels
US20030082571A1 (en) * 2001-04-10 2003-05-01 Kachab Edward Hanna Linear nucleic acid and sequence therefor
US20030191073A1 (en) 2001-11-07 2003-10-09 Challita-Eid Pia M. Nucleic acid and corresponding protein entitled 161P2F10B useful in treatment and detection of cancer
AU2002305019A1 (en) * 2001-05-25 2002-12-09 University Of British Columbia Diagnostic methods for cardiovascular disease, low hdl-cholesterol levels, and high triglyceride levels
US7291457B2 (en) * 2001-06-21 2007-11-06 The Regents Of The University Of California Electrochemical detection of mismatch nucleic acids
US20070098728A1 (en) * 2001-09-24 2007-05-03 Pedersen Finn S Novel compositions and methods in cancer
US20040166490A1 (en) * 2002-12-17 2004-08-26 Morris David W. Novel therapeutic targets in cancer
US20040126762A1 (en) * 2002-12-17 2004-07-01 Morris David W. Novel compositions and methods in cancer
US20040180344A1 (en) * 2003-03-14 2004-09-16 Morris David W. Novel therapeutic targets in cancer
US20040197778A1 (en) * 2002-12-26 2004-10-07 Sagres Discovery, Inc. Novel compositions and methods in cancer
US20060040262A1 (en) * 2002-12-27 2006-02-23 Morris David W Novel compositions and methods in cancer
US6946245B2 (en) * 2001-12-04 2005-09-20 Quest Diagnostics Investments Incorporated Oligonucleotides and methods for detecting hepatitis C viral nucleic acids
US6635428B2 (en) * 2001-12-04 2003-10-21 Quest Diagnostics Investments Incorporated Oligonucleotides and methods for detecting hepatitis B viral nucleic acids
CA2470774A1 (en) * 2001-12-19 2003-06-26 Hvidovre Hospital A method and a kit for determination of a microbial count
US20030175709A1 (en) * 2001-12-20 2003-09-18 Murphy George L. Method and system for depleting rRNA populations
EP1481090A4 (de) * 2002-02-15 2006-08-09 Somalogic Inc Verfahren und reagentien zum nachweis der bindung von zielmolekülen durch nukleinsäureliganden
WO2003071252A2 (en) 2002-02-15 2003-08-28 Exact Sciences Corporation Methods for analysis of molecular events
EP1501855A4 (de) * 2002-03-21 2006-02-22 Sagres Discovery Inc Neue zusammensetzungen und verfahren bei krebs
JP2005521410A (ja) * 2002-03-29 2005-07-21 ジェネンテック・インコーポレーテッド 核酸分析試料の検出と定量化のための方法及び組成物
AU2003243562A1 (en) * 2002-06-12 2003-12-31 Datascope Investment Corp. Polymeric label molecules
US7601493B2 (en) * 2002-07-26 2009-10-13 Nanogen, Inc. Methods and apparatus for screening and detecting multiple genetic mutations
EP1389638A1 (de) * 2002-08-06 2004-02-18 Roche Diagnostics GmbH Verbesserte Proben für FRET
US20040081653A1 (en) 2002-08-16 2004-04-29 Raitano Arthur B. Nucleic acids and corresponding proteins entitled 251P5G2 useful in treatment and detection of cancer
US20060105337A1 (en) * 2002-08-30 2006-05-18 Brian Warner Solid phase based nucleic acid assays combining high affinity and high specificity
AU2003258683A1 (en) * 2002-09-03 2004-03-29 Zeptosens Ag Analytical platform and detection method with analytes which are to be detected in a sample in the form of immobilized specific binding partners
WO2004024949A2 (en) * 2002-09-13 2004-03-25 Hvidovre Hospital Method of rapid detection of mutations and nucleotide polymorphisms using chemometrics
WO2004040605A1 (ja) * 2002-10-31 2004-05-13 Mitsubishi Chemical Corporation 電解コンデンサ用電解液及び電解コンデンサ、並びに有機オニウムのテトラフルオロアルミン酸塩の製造方法
AU2003293367A1 (en) * 2002-12-20 2004-07-29 Stratagene California Compositions and methods for polynucleotide detection
CA2860151A1 (en) 2003-02-10 2004-08-26 Agensys, Inc. Nucleic acid and corresponding protein named 158p1d7 useful in the treatment and detection of bladder and other cancers
EP2058408A3 (de) 2003-02-14 2009-09-09 Sagres Discovery, Inc. Therapeutische GPCR-Targets für Karzinome
US20070218071A1 (en) * 2003-09-15 2007-09-20 Morris David W Novel therapeutic targets in cancer
US20040170982A1 (en) 2003-02-14 2004-09-02 Morris David W. Novel therapeutic targets in cancer
US7767387B2 (en) * 2003-06-13 2010-08-03 Sagres Discovery, Inc. Therapeutic targets in cancer
US6943768B2 (en) 2003-02-21 2005-09-13 Xtellus Inc. Thermal control system for liquid crystal cell
US7229763B2 (en) * 2003-04-07 2007-06-12 Beckman Coulter, Inc. Assay system using labeled oligonucleotides
GB2422196B (en) * 2003-05-20 2008-05-07 Investigen Inc System for detecting polynucleotides
CA2526274C (en) 2003-05-30 2015-12-01 Agensys, Inc. Prostate stem cell antigen (psca) variants and subsequences thereof
US20040259101A1 (en) * 2003-06-20 2004-12-23 Shuber Anthony P. Methods for disease screening
US20040259100A1 (en) 2003-06-20 2004-12-23 Illumina, Inc. Methods and compositions for whole genome amplification and genotyping
DE20310332U1 (de) * 2003-07-04 2004-11-11 Mwg-Biotech Ag Vorrichtung zum automatischen Öffnen und Schließen von Reaktionsgefäßen
US20060035242A1 (en) * 2004-08-13 2006-02-16 Michelitsch Melissa D Prion-specific peptide reagents
WO2005040074A2 (en) * 2003-08-29 2005-05-06 Dendritic Nanotechnologies, Inc. Crick-watson base paired dendrimer structures
US20090163375A1 (en) 2003-09-09 2009-06-25 Bowman Christopher N Use of Photopolymerization for Amplification and Detection of a Molecular Recognition Event
US7354706B2 (en) * 2003-09-09 2008-04-08 The Regents Of The University Of Colorado, A Body Corporate Use of photopolymerization for amplification and detection of a molecular recognition event
US20050064435A1 (en) * 2003-09-24 2005-03-24 Xing Su Programmable molecular barcodes
US20070281896A1 (en) * 2003-09-30 2007-12-06 Morris David W Novel compositions and methods in cancer
US20050136414A1 (en) * 2003-12-23 2005-06-23 Kevin Gunderson Methods and compositions for making locus-specific arrays
US20050250147A1 (en) * 2004-05-10 2005-11-10 Macevicz Stephen C Digital profiling of polynucleotide populations
WO2005111244A2 (en) * 2004-05-10 2005-11-24 Exact Sciences Corporation Methods for detecting a mutant nucleic acid
EP2322650A1 (de) 2004-05-14 2011-05-18 Rosetta Genomics Ltd Mikro-RNAs und deren Verwendung
EP2322662A1 (de) 2004-05-14 2011-05-18 Rosetta Genomics Ltd Mikro-RNAs und deren Verwendung
WO2005113769A1 (en) * 2004-05-14 2005-12-01 Exact Sciences Corporation Method for stabilizing biological samples for nucleic acid analysis
EP1804059A2 (de) 2004-05-21 2007-07-04 Atonomics A/S Oberflächenschallwellensensor mit einem Hydrogel
EP1753871B1 (de) 2004-05-28 2015-07-15 Agensys, Inc. An psca-proteine bindende antikörper und verwandte moleküle
US8034554B2 (en) 2004-07-01 2011-10-11 Gen-Probe Incorporated Methods and compositions to detect nucleic acids in a biological sample
US20060024677A1 (en) 2004-07-20 2006-02-02 Morris David W Novel therapeutic targets in cancer
US7981607B2 (en) * 2004-08-27 2011-07-19 Esoterix Genetic Laboratories LLC Method for detecting recombinant event
US7482122B1 (en) * 2004-10-19 2009-01-27 Hai Xing Chen Method of signal enhancement for measurement of nucleic acid sequences
WO2006047787A2 (en) 2004-10-27 2006-05-04 Exact Sciences Corporation Method for monitoring disease progression or recurrence
AU2005333156B2 (en) * 2004-11-03 2011-05-26 Iris Molecular Diagnostics, Inc. Homogeneous analyte detection
WO2006076683A2 (en) * 2005-01-13 2006-07-20 Novartis Vaccines And Diagnostics Inc. Isolation and detection of pathogenic prions
EP2348320B1 (de) 2005-03-10 2024-05-01 Gen-Probe Incorporated Verfahren und Systeme zur Erkennung mehrerer Fluoreszenzemissionssignale
WO2006099255A2 (en) 2005-03-10 2006-09-21 Gen-Probe Incorporated Systems and methods to perform assays for detecting or quantifying analytes within samples
EP3300739A3 (de) 2005-03-31 2018-07-18 Agensys, Inc. An 161p2f10b-proteine bindende antikörper und zugehörige moleküle
US20090214536A1 (en) 2005-04-07 2009-08-27 Guoying Yu CACNA1E in Cancer Diagnosis, Detection and Treatment
WO2006110585A2 (en) 2005-04-07 2006-10-19 Novartis Vaccines And Diagnostics Inc. Cancer-related genes (prlr)
WO2007044071A2 (en) * 2005-04-21 2007-04-19 Exact Sciences Corporation Analysis of heterogeneous nucleic acid samples
ES2381279T3 (es) 2005-05-06 2012-05-24 Gen-Probe Incorporated Métodos y productos para la captura de ácido nucleico diana
EP1880206B1 (de) 2005-05-09 2012-11-14 Affymetrix, Inc. Multiplex-einfang von nukleinsäuren
US8632970B2 (en) 2005-05-09 2014-01-21 Affymetrix, Inc. Multiplex capture of nucleic acids
WO2006124771A2 (en) * 2005-05-12 2006-11-23 Panomics, Inc. Multiplex branched-chain dna assays
US20090081688A1 (en) * 2005-06-20 2009-03-26 Advanced Cell Diagnostics Methods of detecting nucleic acids in individual cells and of identifying rare cells from large heterogeneous cell populations
US20080038725A1 (en) 2005-06-20 2008-02-14 Yuling Luo Methods of detecting nucleic acids in individual cells and of identifying rare cells from large heterogeneous cell populations
KR20080047355A (ko) * 2005-07-06 2008-05-28 바이오칩 이노베이션즈 피티와이 리미티드 표적 핵산 서열 분석 방법 및 키트
WO2007019444A2 (en) * 2005-08-05 2007-02-15 Euclid Diagnostics Llc Subtractive separation and amplification of non-ribosomal transcribed rna (nrrna)
US7410763B2 (en) * 2005-09-01 2008-08-12 Intel Corporation Multiplex data collection and analysis in bioanalyte detection
US20120231453A1 (en) * 2005-09-13 2012-09-13 Affymetrix, Inc. Brownian Microbarcodes for Bioassays
US7927798B2 (en) * 2005-10-05 2011-04-19 Panomics, Inc. Detection of nucleic acids from whole blood
EP1951905B1 (de) 2005-11-10 2012-04-11 Affymetrix, Inc. Nachweis von nukleinsäuren über amplifikation von stellvertreternukleinsäuren
WO2007087312A2 (en) * 2006-01-23 2007-08-02 Population Genetics Technologies Ltd. Molecular counting
WO2007100711A2 (en) * 2006-02-24 2007-09-07 Investigen, Inc. Methods and compositions for detecting polynucleotides
EP2411530A2 (de) 2006-04-26 2012-02-01 Bayer Technology Services GmbH Nukleinsäure-testverfahren auf festphasenbasis mit kombination aus hochaffinem einfang und nachweis über spezifische hybridisierung
CA2672246A1 (en) 2006-06-30 2008-03-13 Rosetta Genomics Ltd Detecting nucleic acids
JP2008043332A (ja) * 2006-08-17 2008-02-28 Panomics Inc 組織スライドからの核酸定量
JP2008064475A (ja) * 2006-09-04 2008-03-21 Osaka Univ 標的物質の高感度検出方法、検出用キットおよび検出装置
WO2008052774A2 (en) * 2006-10-31 2008-05-08 Noxxon Pharma Ag Methods for detection of a single- or double-stranded nucleic acid molecule
US7615351B2 (en) * 2006-12-01 2009-11-10 Panomics, Inc. Two stage nucleic acid amplification using an amplification oligomer
WO2009024834A2 (en) * 2006-12-05 2009-02-26 Rosetta Genomics Ltd Nucleic acids involved in viral infection
US20110136099A1 (en) * 2007-01-16 2011-06-09 Somalogic, Inc. Multiplexed Analyses of Test Samples
US7964356B2 (en) * 2007-01-16 2011-06-21 Somalogic, Inc. Method for generating aptamers with improved off-rates
US7855054B2 (en) * 2007-01-16 2010-12-21 Somalogic, Inc. Multiplexed analyses of test samples
US8975026B2 (en) 2007-01-16 2015-03-10 Somalogic, Inc. Method for generating aptamers with improved off-rates
US7947447B2 (en) 2007-01-16 2011-05-24 Somalogic, Inc. Method for generating aptamers with improved off-rates
US20080241140A1 (en) 2007-02-12 2008-10-02 Medical College Of Georgia Gene amplification of coactivator coaa and uses thereof
WO2008103702A2 (en) * 2007-02-23 2008-08-28 Investigen, Inc. Methods and compositions for rapid light-activated isolation and detection of analytes
JP5211790B2 (ja) * 2007-03-26 2013-06-12 住友化学株式会社 Ｄｎａメチル化測定方法
US7794985B2 (en) * 2007-04-04 2010-09-14 Ghc Technologies, Inc. Methods and compositions for rapid amplification, capture and detection of nucleic acids and proteins
US20100047792A1 (en) * 2007-04-10 2010-02-25 Szendroe Istvan Label-free optical detection method
US8450058B2 (en) * 2007-08-14 2013-05-28 Eisai R&D Management Co., Ltd. Method of detecting target substance
EP2511708B1 (de) 2007-10-05 2016-09-14 Affymetrix, Inc. Assays auf der Basis hoch gebündelter Partikel
US8906700B2 (en) 2007-11-06 2014-12-09 Ambergen, Inc. Methods and compositions for phototransfer
US20090137405A1 (en) * 2007-11-16 2009-05-28 Christopher Bowman Detection of nucleic acid biomarkers using polymerization-based amplification
US20090162845A1 (en) * 2007-12-20 2009-06-25 Elazar Rabbani Affinity tag nucleic acid and protein compositions, and processes for using same
US8538733B2 (en) * 2008-01-25 2013-09-17 Life Technologies Corporation Methods for the analysis of dissociation melt curve data
CA2721982A1 (en) * 2008-04-30 2009-11-05 Novartis Ag Assay for pathogenic conformers
EP2806037B1 (de) 2008-05-13 2016-09-21 Gen-Probe Incorporated Inaktivierbare Target-Capture-Oligomere zur Verwendung bei der selektiven Hybridisierung und beim Fangen von Zielnukleinsäuresequenzen
US20090298709A1 (en) * 2008-05-28 2009-12-03 Affymetrix, Inc. Assays for determining telomere length and repeated sequence copy number
US8703416B2 (en) 2008-07-17 2014-04-22 Somalogic, Inc. Method for purification and identification of sperm cells
EP2350648B8 (de) * 2008-09-22 2017-07-19 Ventana Medical Systems, Inc. Selektive verarbeitung biologischer materialien auf einem mikroarray-substrat
KR20100042082A (ko) * 2008-10-15 2010-04-23 삼성전자주식회사 신호물질의 밀도가 증가되어 있는 고체 지지체, 그를 포함하는 키트 및 그를 이용한 표적물질 검출 방법
JP2012506705A (ja) 2008-10-27 2012-03-22 キアジェン ゲイサーズバーグ インコーポレイテッド 迅速に結果を得るハイブリッドキャプチャー法による分析およびシステム
US8309306B2 (en) * 2008-11-12 2012-11-13 Nodality, Inc. Detection composition
JP5850746B2 (ja) * 2008-11-17 2016-02-03 ヘッドウェイ テクノロジーズ, インク.Headway Technologies, Inc. 連結分子を用いた標的分子の粒子ベースの検出における方法および組成物
EP2358898B1 (de) 2008-11-17 2016-08-31 Headway Technologies, Inc. Verfahren und zusammensetzungen bei dem nachweis von zielmolekülen auf partikelbasis unter verwendung von kovalente bindungen ausbildenden reaktiven paaren
DK2189539T4 (en) * 2008-11-21 2018-09-17 Chimera Biotec Gmbh Conjugate complexes for analyte detection
CN102292457B (zh) 2008-11-25 2014-04-09 简·探针公司 检测小rna的组合物和方法及其用途
CN102257160A (zh) * 2008-12-18 2011-11-23 西门子医疗保健诊断公司 用于缩短杂交分析中的培养时间的方法和试剂
CA2750338C (en) * 2009-01-28 2019-06-25 Qiagen Gaithersburg, Inc. Sequence-specific large volume sample preparation method and assay
EP3021120A1 (de) 2009-02-20 2016-05-18 Michael P. Lisanti Verfahren zur diagnose oder prognose eines neoplasmas einschliesslich der bestimmung des expressionsgrads eines proteins in stromazellen neben dem neoplasma
CN102414327B (zh) 2009-05-01 2015-04-08 奇亚根盖瑟斯堡股份有限公司 用于在样品中检测rna剪接形式的非靶向扩增方法
WO2013132700A1 (ja) * 2012-03-05 2013-09-12 日本碍子株式会社 標的核酸の検出方法
WO2011032124A1 (en) 2009-09-14 2011-03-17 Qiagen Gaithersburg, Inc. Compositions and methods for recovery of nucleic acids or proteins from tissue samples fixed in cytology media
WO2011038403A1 (en) 2009-09-28 2011-03-31 Yuling Luo Methods of detecting nucleic acid sequences with high specificity
CN102725639A (zh) 2009-11-04 2012-10-10 诺华有限公司 蛋白质聚集物与单体分离中作为结合试剂的带正电物质
WO2011071923A2 (en) 2009-12-07 2011-06-16 Illumina, Inc. Multi-sample indexing for multiplex genotyping
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
US9315857B2 (en) 2009-12-15 2016-04-19 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse label-tags
US8501490B2 (en) * 2010-01-08 2013-08-06 The Regents Of The University Of California Bioassays based on polymeric sequence probe
JP6103941B2 (ja) * 2010-01-29 2017-03-29 キアジェン ゲイサーズバーグ インコーポレイテッド 核酸の配列特異的精製および多重分析のための方法および組成物
JP2013517803A (ja) 2010-01-29 2013-05-20 キアジェン ゲイサーズバーグ インコーポレイテッド サンプル中のｈｐｖの存在を決定および確認する方法
EP2529030B1 (de) 2010-01-29 2019-03-13 Advanced Cell Diagnostics, Inc. Verfahren zum in situ nachweis von nukleinsäuren
EP2539355B1 (de) * 2010-02-26 2016-10-05 Ventana Medical Systems, Inc. In-situ hybridisierung mit polytag-sonden
CA2799200A1 (en) 2010-05-19 2011-11-24 Qiagen Gaithersburg, Inc. Methods and compositions for sequence-specific purification and multiplex analysis of nucleic acids
US9376727B2 (en) 2010-05-25 2016-06-28 Qiagen Gaithersburg, Inc. Fast results hybrid capture assay and associated strategically truncated probes
US20120004132A1 (en) * 2010-07-02 2012-01-05 Affymetrix, Inc. Detection of Nucleic Acids and Proteins
ES2523140T3 (es) 2010-09-21 2014-11-21 Population Genetics Technologies Ltd. Aumento de la confianza en las identificaciones de alelos con el recuento molecular
EP3034625B1 (de) 2010-10-21 2017-10-04 Advanced Cell Diagnostics, Inc. Hochsensitives verfahren für den in-situ-nachweis von nukleinsäuren
JP5960709B2 (ja) 2010-10-27 2016-08-02 プレジデント アンド フェローズ オブ ハーバード カレッジ トーホールドプライマーデュプレックスの組成物およびその使用方法
EP3388532B1 (de) * 2010-11-01 2021-03-10 Gen-Probe Incorporated Integrierte erfassung und amplifizierung von zielnukleinsäuren zur sequenzierung
US20120178081A1 (en) * 2010-12-31 2012-07-12 Affymetrix. Inc. Methods of Labeling Cells, Labeled Cells, and uses Thereof
JP6126997B2 (ja) 2011-02-16 2017-05-10 ヘッドウェイ テクノロジーズ, インク.Headway Technologies, Inc. 検出可能な標識の標的限局性の係留のための方法及び組成物
WO2012116220A2 (en) 2011-02-24 2012-08-30 Qiagen Gaithersburg, Inc. Materials and methods for detection of hpv nucleic acid
US9335292B2 (en) 2011-10-13 2016-05-10 Auburn University Electrochemical proximity assay
EP3604555B1 (de) 2011-10-14 2024-12-25 President and Fellows of Harvard College Sequenzierung durch strukturanordnung
JP6430253B2 (ja) * 2011-11-05 2018-11-28 プレジデント アンド フェローズ オブ ハーバード カレッジ 相互作用を検出および測定するための核酸ベースのリンカー
EP2783016A1 (de) 2011-11-22 2014-10-01 Intermune, Inc. Verfahren zur diagnose und behandlung von idiopathischer lungenfibrose
WO2014163886A1 (en) 2013-03-12 2014-10-09 President And Fellows Of Harvard College Method of generating a three-dimensional nucleic acid containing matrix
ES2991004T3 (es) 2011-12-22 2024-12-02 Harvard College Métodos para la detección de analitos
US10385392B2 (en) * 2011-12-30 2019-08-20 Abbott Molecular Inc. Nucleic acid hybridization probes
GB2523211B (en) 2012-01-27 2020-03-18 Univ Jefferson MCT protein inhibitor-related prognostic and therapeutic methods
CA2865575C (en) 2012-02-27 2024-01-16 Cellular Research, Inc. Compositions and kits for molecular counting
US11177020B2 (en) 2012-02-27 2021-11-16 The University Of North Carolina At Chapel Hill Methods and uses for molecular tags
US9670529B2 (en) 2012-02-28 2017-06-06 Population Genetics Technologies Ltd. Method for attaching a counter sequence to a nucleic acid sample
US20140178869A1 (en) 2012-04-05 2014-06-26 Advanced Cell Diagnostics, Inc. Detection of immunoglobulin light chain restriction by rna in situ hybridization
WO2013184754A2 (en) 2012-06-05 2013-12-12 President And Fellows Of Harvard College Spatial sequencing of nucleic acids using dna origami probes
US10876152B2 (en) 2012-09-04 2020-12-29 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
US20160040229A1 (en) 2013-08-16 2016-02-11 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
IL269097B2 (en) 2012-09-04 2024-01-01 Guardant Health Inc Systems and methods to detect rare mutations and copy number variation
US11913065B2 (en) 2012-09-04 2024-02-27 Guardent Health, Inc. Systems and methods to detect rare mutations and copy number variation
US9783841B2 (en) 2012-10-04 2017-10-10 The Board Of Trustees Of The Leland Stanford Junior University Detection of target nucleic acids in a cellular sample
WO2014074785A1 (en) 2012-11-08 2014-05-15 Ludwig Institute For Cancer Research Ltd. Methods of predicting outcome and treating breast cancer
US20160024565A1 (en) * 2013-03-13 2016-01-28 Tufts University Fragment complementation of based assays
US9273349B2 (en) 2013-03-14 2016-03-01 Affymetrix, Inc. Detection of nucleic acids
US9255265B2 (en) 2013-03-15 2016-02-09 Illumina, Inc. Methods for producing stranded cDNA libraries
WO2014172627A1 (en) 2013-04-19 2014-10-23 Thomas Jefferson University Caveolin-1 related methods for treating glioblastoma with temozolomide
US20140371088A1 (en) * 2013-06-14 2014-12-18 Nanostring Technologies, Inc. Multiplexable tag-based reporter system
US9745633B2 (en) * 2013-06-28 2017-08-29 Diacarta Ltd Detection of PNA clamping
DE102013011304A1 (de) * 2013-07-02 2015-01-22 Technische Universität Dresden Verfahren und Anordnung zur Erfassung von Bindungsereignissen von Molekülen
ES2857908T3 (es) 2013-08-28 2021-09-29 Becton Dickinson Co Análisis masivamente paralelo de células individuales
CN105745528A (zh) 2013-10-07 2016-07-06 赛卢拉研究公司 用于以数字方式对阵列上的特征进行计数的方法和系统
EP3378952B1 (de) 2013-12-28 2020-02-05 Guardant Health, Inc. Verfahren und systeme für den nachweis genetischer varianten
WO2016007839A1 (en) 2014-07-11 2016-01-14 President And Fellows Of Harvard College Methods for high-throughput labelling and detection of biological features in situ using microscopy
WO2016089588A1 (en) 2014-12-06 2016-06-09 Children's Medical Center Corporation Nucleic acid-based linkers for detecting and measuring interactions
US11639522B2 (en) 2015-01-30 2023-05-02 President And Fellows Of Harvard College Microscope-free imaging
WO2016134078A1 (en) 2015-02-19 2016-08-25 Becton, Dickinson And Company High-throughput single-cell analysis combining proteomic and genomic information
WO2016138496A1 (en) 2015-02-27 2016-09-01 Cellular Research, Inc. Spatially addressable molecular barcoding
ES2934982T3 (es) 2015-03-30 2023-02-28 Becton Dickinson Co Métodos para la codificación con códigos de barras combinatorios
US11390914B2 (en) 2015-04-23 2022-07-19 Becton, Dickinson And Company Methods and compositions for whole transcriptome amplification
WO2016196229A1 (en) 2015-06-01 2016-12-08 Cellular Research, Inc. Methods for rna quantification
US11396650B2 (en) 2015-06-02 2022-07-26 Children's Medical Center Corporation Nucleic acid complexes for screening barcoded compounds
US12077807B2 (en) 2015-06-27 2024-09-03 The Research Foundation For The State University Of New York Compositions and methods for analyte detection using nanoswitches
EP3325650B1 (de) 2015-07-17 2024-01-03 Nanostring Technologies, Inc. Simultane quantifizierung der genexpression in einer benutzerdefinierten region eines gewebequerschnitts
AU2016297513B2 (en) 2015-07-17 2021-02-25 Board Of Regents, The University Of Texas System Simultaneous quantification of a plurality of proteins in a user-defined region of a cross-sectioned tissue
CN108026524A (zh) 2015-09-11 2018-05-11 赛卢拉研究公司 用于核酸文库标准化的方法和组合物
EP3362462B1 (de) 2015-10-12 2021-07-21 Advanced Cell Diagnostics, Inc. In-situ-detektion von nukleotidvarianten bei proben mit hohem rauschen sowie zugehörige zusammensetzungen und verfahren
EP3371329B1 (de) 2015-11-03 2026-03-25 President and Fellows of Harvard College Verfahren und vorrichtung zur volumetrischen bildgebung einer dreidimensionalen nukleinsäurehaltigen matrix
WO2017106768A1 (en) 2015-12-17 2017-06-22 Guardant Health, Inc. Methods to determine tumor gene copy number by analysis of cell-free dna
US11713483B2 (en) 2016-02-09 2023-08-01 Children's Medical Center Corporation Method for detection of analytes via polymer complexes
WO2017165585A1 (en) 2016-03-23 2017-09-28 Children's Medical Center Corporation Systems and apparatus for detecting compounds in human biological samples
WO2017165647A1 (en) 2016-03-23 2017-09-28 Children's Medical Center Corporation Rapid and sensitive detection and quantification of analytes in complex samples using polymer-based methods
CA3210120C (en) * 2016-04-25 2024-04-09 President And Fellows Of Harvard College Hybridization chain reaction methods for in situ molecular detection
CA3019589A1 (en) 2016-05-02 2017-11-09 Cellular Research, Inc. Accurate molecular barcoding
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
CN109074430B (zh) 2016-05-26 2022-03-29 贝克顿迪金森公司 分子标记计数调整方法
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
EP3481843B1 (de) 2016-07-05 2022-01-26 California Institute of Technology Hybridisierungskettenreaktion mit fraktioniertem initiator
GB2569252A (en) 2016-08-31 2019-06-12 Harvard College Methods of combining the detection of biomolecules into a single assay using fluorescent in situ sequencing
KR102363716B1 (ko) 2016-09-26 2022-02-18 셀룰러 리서치, 인크. 바코딩된 올리고뉴클레오티드 서열을 갖는 시약을 이용한 단백질 발현의 측정
US11608497B2 (en) 2016-11-08 2023-03-21 Becton, Dickinson And Company Methods for cell label classification
JP7232180B2 (ja) 2016-11-08 2023-03-02 ベクトン・ディキンソン・アンド・カンパニー 発現プロファイル分類の方法
KR102476709B1 (ko) 2016-11-21 2022-12-09 나노스트링 테크놀로지스, 인크. 화학적 조성물 및 이것을 사용하는 방법
JP7093355B2 (ja) * 2016-12-16 2022-06-29 アラトム， エルエルシー ライゲーション増幅を使用する分子検出
JP7116062B2 (ja) * 2017-01-10 2022-08-09 プレジデント アンド フェローズ オブ ハーバード カレッジ 多重化シグナル増幅
JP7104048B2 (ja) 2017-01-13 2022-07-20 セルラー リサーチ， インコーポレイテッド 流体チャネルの親水性コーティング
US11319583B2 (en) 2017-02-01 2022-05-03 Becton, Dickinson And Company Selective amplification using blocking oligonucleotides
US10852274B2 (en) 2017-03-09 2020-12-01 Auburn University Differential circuit for background correction in electrochemical measurements
WO2018226293A1 (en) 2017-06-05 2018-12-13 Becton, Dickinson And Company Sample indexing for single cells
CN107227350A (zh) * 2017-06-09 2017-10-03 苏州达麦迪生物医学科技有限公司 一种快速检测alk基因断裂的探针组、试剂盒及方法
US11505799B2 (en) 2017-07-07 2022-11-22 Innamed, Inc. Aptamers for measuring lipoprotein levels
EP4589017A3 (de) 2017-10-06 2025-10-08 10x Genomics, Inc. Rna-template-ligation
US11591636B2 (en) 2017-11-20 2023-02-28 Children's Medical Center Corporation Force-controlled nanoswitch assays for single-molecule detection in complex biological fluids
CN111492068B (zh) 2017-12-19 2025-03-21 贝克顿迪金森公司 与寡核苷酸相关联的颗粒
CN111630179B (zh) 2018-01-26 2024-07-19 哈佛学院院长及董事 邻近检测方法和组合物
WO2019157445A1 (en) 2018-02-12 2019-08-15 Nanostring Technologies, Inc. Biomolecular probes and methods of detecting gene and protein expression
WO2019199643A1 (en) 2018-04-09 2019-10-17 Bio-Techne Corporation Methods to further enhance signal amplification for the in situ detection of nucleic acids
WO2019213294A1 (en) 2018-05-03 2019-11-07 Becton, Dickinson And Company High throughput multiomics sample analysis
WO2019213237A1 (en) 2018-05-03 2019-11-07 Becton, Dickinson And Company Molecular barcoding on opposite transcript ends
EP3794146B1 (de) 2018-05-14 2025-12-10 Bruker Spatial Biology, Inc. Verfahren zur erkennung einer zuvor bestimmten nukleotidesequenz
US11560565B2 (en) 2018-06-13 2023-01-24 Auburn University Electrochemical detection nanostructure, systems, and uses thereof
CN118853827A (zh) 2018-10-01 2024-10-29 贝克顿迪金森公司 确定5’转录物序列
WO2020076976A1 (en) 2018-10-10 2020-04-16 Readcoor, Inc. Three-dimensional spatial molecular indexing
CN112969789A (zh) 2018-11-08 2021-06-15 贝克顿迪金森公司 使用随机引发的单细胞全转录组分析
EP3894552B1 (de) 2018-12-13 2026-01-28 Becton, Dickinson and Company Selektive verlängerung in der vollständigen transkriptomanalyse von einzelzellen
US11371076B2 (en) 2019-01-16 2022-06-28 Becton, Dickinson And Company Polymerase chain reaction normalization through primer titration
ES2945227T3 (es) 2019-01-23 2023-06-29 Becton Dickinson Co Oligonucleótidos asociados con anticuerpos
EP3924506A1 (de) 2019-02-14 2021-12-22 Becton Dickinson and Company Hybride gezielte und gesamttranskriptomamplifikation
US12571028B2 (en) 2019-02-15 2026-03-10 Advanced Cell Diagnostics, Inc. Methods for multiplex detection of nucleic acids by in situ hybridization
US11965208B2 (en) 2019-04-19 2024-04-23 Becton, Dickinson And Company Methods of associating phenotypical data and single cell sequencing data
AU2020282024A1 (en) 2019-05-31 2021-11-11 10X Genomics, Inc. Method of detecting target nucleic acid molecules
CN114051534B (zh) 2019-07-22 2025-02-21 贝克顿迪金森公司 单细胞染色质免疫沉淀测序测定
CN114729350A (zh) 2019-11-08 2022-07-08 贝克顿迪金森公司 使用随机引发获得用于免疫组库测序的全长v(d)j信息
GB201919032D0 (en) 2019-12-20 2020-02-05 Cartana Ab Method of detecting an analyte
EP4090763B1 (de) 2020-01-13 2024-12-04 Becton Dickinson and Company Verfahren und zusammensetzungen zur quantifizierung von proteinen und rna
WO2021155063A1 (en) 2020-01-29 2021-08-05 Readcoor, Llc Compositions and methods for analyte detection
ES2993319T3 (en) 2020-01-29 2024-12-27 Becton Dickinson Co Barcoded wells for spatial mapping of single cells through sequencing
US12110548B2 (en) 2020-02-03 2024-10-08 10X Genomics, Inc. Bi-directional in situ analysis
US12215379B2 (en) 2020-02-17 2025-02-04 10X Genomics, Inc. In situ analysis of chromatin interaction
JP2023514749A (ja) 2020-02-21 2023-04-07 １０エックス ジェノミクス インコーポレイテッド 統合型インサイチュ空間的アッセイのための方法および組成物
EP4111168A1 (de) 2020-02-25 2023-01-04 Becton Dickinson and Company Bispezifische sonden zur ermöglichung der verwendung von einzelzellenproben als einzelfarbkompensationssteuerung
WO2021174071A1 (en) 2020-02-29 2021-09-02 President And Fellows Of Harvard College Nanoswitch caliper trains for high-throughput, high-resolution structural analysis of biomolecules
US12188085B2 (en) 2020-03-05 2025-01-07 10X Genomics, Inc. Three-dimensional spatial transcriptomics with sequencing readout
JP2023521356A (ja) 2020-04-07 2023-05-24 アドヴァンスド セル ダイアグノスティクス，インコーポレイテッド ｉｎ ｓｉｔｕハイブリダイゼーションによって標的核酸を検出する方法及びそのキット
CN115605614A (zh) 2020-05-14 2023-01-13 贝克顿迪金森公司（Us） 用于免疫组库谱分析的引物
WO2021247593A1 (en) 2020-06-02 2021-12-09 Becton, Dickinson And Company Oligonucleotides and beads for 5 prime gene expression assay
US12209273B2 (en) 2020-06-12 2025-01-28 10X Genomics, Inc. Nucleic acid assays using click chemistry bioconjugation
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
US12391940B2 (en) 2020-07-31 2025-08-19 Becton, Dickinson And Company Single cell assay for transposase-accessible chromatin
US12297499B2 (en) 2020-08-17 2025-05-13 10X Genomics, Inc. Multicomponent nucleic acid probes for sample analysis
US20220049303A1 (en) 2020-08-17 2022-02-17 Readcoor, Llc Methods and systems for spatial mapping of genetic variants
WO2022040455A1 (en) 2020-08-21 2022-02-24 Advanced Cell Diagnostics, Inc. Compositions and methods for determining t cell clonality
US12071667B2 (en) 2020-11-04 2024-08-27 10X Genomics, Inc. Sequence analysis using meta-stable nucleic acid molecules
WO2022109343A1 (en) 2020-11-20 2022-05-27 Becton, Dickinson And Company Profiling of highly expressed and lowly expressed proteins
EP4012046A1 (de) 2020-12-11 2022-06-15 10X Genomics, Inc. Verfahren und zusammensetzungen zur multimodalen in-situ-analyse
EP4263859A1 (de) 2020-12-15 2023-10-25 Becton, Dickinson and Company Einzelzellensekretomanalyse
US12060603B2 (en) 2021-01-19 2024-08-13 10X Genomics, Inc. Methods for internally controlled in situ assays using padlock probes
EP4284925A4 (de) 2021-01-26 2025-08-13 California Inst Of Techn Allosterische bedingte guide-rnas zur zellselektiven regulierung von crispr/cas
US12275984B2 (en) 2021-03-02 2025-04-15 10X Genomics, Inc. Sequential hybridization and quenching
EP4347874A1 (de) * 2021-05-24 2024-04-10 California Institute of Technology Verknüpfte amplifikation mit exponentieller strahlung
US12139751B2 (en) 2021-07-30 2024-11-12 10X Genomics, Inc. Circularizable probes for in situ analysis
ES2988400T3 (es) 2021-07-30 2024-11-20 10X Genomics Inc Métodos y composiciones para sincronizar reacciones in situ
US12529096B2 (en) 2021-08-03 2026-01-20 10X Genomics, Inc. Stabilization and/or compaction of nucleic acid structures
WO2023015192A1 (en) 2021-08-03 2023-02-09 10X Genomics, Inc. Nucleic acid concatemers and methods for stabilizing and/or compacting the same
US12460251B2 (en) 2021-08-03 2025-11-04 10X Genomics, Inc. Stabilization and/or compaction of nucleic acid molecules
US12391984B2 (en) 2021-08-03 2025-08-19 10X Genomics, Inc. Compositions and methods for rolling circle amplification
EP4446426B1 (de) 2021-08-16 2026-03-04 10X Genomics, Inc. Sonden mit einem geteilten strichcodebereich und verfahren zur verwendung
EP4466376B1 (de) 2022-01-21 2026-03-04 10X Genomics, Inc. Mehrere auslesesignale zur analyse einer probe
EP4490737A1 (de) 2022-03-08 2025-01-15 10X Genomics, Inc. In-situ-codedesignverfahren zur minimierung von optischem crowding
CN119032271A (zh) 2022-04-01 2024-11-26 10X基因组学有限公司 用于靶向掩蔽自发荧光的组合物和方法
WO2023215603A1 (en) 2022-05-06 2023-11-09 10X Genomics, Inc. Methods and compositions for in situ analysis of v(d)j sequences
US20240084378A1 (en) 2022-05-11 2024-03-14 10X Genomics, Inc. Compositions and methods for in situ sequencing
EP4540607A1 (de) 2022-06-17 2025-04-23 10X Genomics, Inc. Katalytische entvernetzung von proben zur in-situ-analyse
CN119677873A (zh) 2022-08-16 2025-03-21 10X基因组学有限公司 Ap50聚合酶及其用途
WO2024124041A1 (en) 2022-12-07 2024-06-13 Advanced Cell Diagnostics, Inc. Multiplexed detection of nucleic acid targets
WO2025029831A1 (en) 2023-07-31 2025-02-06 10X Genomics, Inc. Methods and systems for targeted rna cleavage and target rna-primed rolling circle amplification

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1190838A (en) * 1981-07-17 1985-07-23 Cavit Akin Homogeneous nucleic acid hybridization diagnostics by non-radiative energy transfer
CA1260372A (en) * 1984-04-27 1989-09-26 Elazar Rabbani Hybridization method for the detection of genetic materials
US4868105A (en) * 1985-12-11 1989-09-19 Chiron Corporation Solution phase nucleic acid sandwich assay
US5175270A (en) * 1986-09-10 1992-12-29 Polyprobe, Inc. Reagents for detecting and assaying nucleic acid sequences
US5124246A (en) * 1987-10-15 1992-06-23 Chiron Corporation Nucleic acid multimers and amplified nucleic acid hybridization assays using same
JP3276955B2 (ja) * 1988-09-30 2002-04-22 ジーン−トラック・システムス Ｒｎａの鋳型の末端に結合したプローブ構造およびその使用方法
EP0481999B1 (de) * 1989-03-10 1997-07-23 VYSIS, Inc. Interferenzverhinderung mit affinitätseinfangschemas
US5595891A (en) * 1990-07-19 1997-01-21 Behringwerke Ag Method for producing a polynucleotide for use in single primer amplification
JPH07502414A (ja) * 1991-12-23 1995-03-16 バイエル　コーポレイション 溶液相サンドイッチハイブリダイゼーションアッセイに用いるためのクラミジアプローブ
CA2124797C (en) * 1991-12-23 2004-03-30 Bruce D. Irvine Hiv probes for use in solution phase sandwich hybridization assays
JPH07502655A (ja) * 1991-12-23 1995-03-23 バイエル　コーポレイション 溶液相サンドイッチハイブリダイゼーションアッセイに用いるためのｃｍｖプローブ
US5424413A (en) * 1992-01-22 1995-06-13 Gen-Probe Incorporated Branched nucleic acid probes

Also Published As

Publication number Publication date
EP0731848B1 (de) 2003-05-21
EP0731848A1 (de) 1996-09-18
HU221237B1 (en) 2002-08-28
CA2178598A1 (en) 1995-06-15
US5635352A (en) 1997-06-03
JP3892450B2 (ja) 2007-03-14
AU1303895A (en) 1995-06-27
WO1995016055A1 (en) 1995-06-15
ES2199236T3 (es) 2004-02-16
HUT74225A (en) 1996-11-28
KR960706566A (ko) 1996-12-09
US5681697A (en) 1997-10-28
KR100327610B1 (ko) 2002-07-02
HU9601593D0 (en) 1996-07-29
DE69432709T2 (de) 2003-11-20
DE69432709D1 (de) 2003-06-26
JPH09507024A (ja) 1997-07-15
JP2006020649A (ja) 2006-01-26
JP3802925B2 (ja) 2006-08-02
JP2006055170A (ja) 2006-03-02
JP2004301849A (ja) 2004-10-28
AU694468B2 (en) 1998-07-23

Similar Documents

Publication Publication Date Title
ATE241017T1 (de) Nukleinsäure - sandwichassay in lösungsphase mit reduzierten hintergrundsignalen
EP0915173A3 (de) Reagentiensatz und Verfahren für Fluoreszenzerkennungstest
AU582293B2 (en) Heterogeneous specific binding assay employing an aggregatable binding reagent
AU7974987A (en) Immunoassay based on two ensyme inhibition system
EP0256932A3 (en) Use of oxidase enzyme systems in chemiluminescent assays
ES2173292T3 (es) Reactivos de apagado y ebsayos de luminiscencia mediada por enzimas.
EP0276152A3 (de) Immunoassays und Vorrichtungen gekennzeichnet durch orthographischen Fluss
ES2136059T3 (es) Deteccion de una secuencia de acidos nucleicos o de un cambio en la misma.
WO2004059294A3 (en) Improved luciferase-based assays
BG97041A (bg) Проби на база протеин-нуклеинова киселина и метод за използването им при имуноизследване
CA2205135A1 (en) Novel 1,2-dioxetane compounds with haloalkoxy groups, methods of preparation and use
EP0982592A3 (de) Verbessertes Verfahren zur Bestimmung von Estradiol in einer Probenflüssigkeit und Kit
KR920004839A (ko) 1종 이상의 분석물을 검출하기 위한 동시 검정법
EP0402846A3 (de) Fluoreszenzpolarisationsimmunoassay für Tetrahydrocannabinoide
AU6453899A (en) Method for detecting an analyte and kit
DE69516415D1 (de) Porphyrin-markierung von polynukleotiden
BR9609390A (pt) Ensaio quimiluminescente de ligação específica com não-separação
EP0304845A3 (de) Verfahren und Zusammensetzung zum Nachweis der Genexpression
DE69016617D1 (de) Gepufferte Waschzusammensetzung, Zusammensetzung zur Unlöslichmachung, Testsätze und Verfahren zu ihrer Verwendung.
AU7276891A (en) Immunological detection of metolachlor
ATE292283T1 (de) Verfahren zur immobilisierung von affinen reagentien an hydrophoben festphasen
Kessler Non-radioactive analysis of biomolecules
CA2425483A1 (en) Assay method utilizing induced luminescence
DK0815259T3 (da) Modulation af nucleinsyre-bindingspartneres bindingsegenskaber
GB2335038A (en) Double label immunoassay

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties