ATE341621T1 - Rekombinatorisches klonieren unter verwendung von nukleinsaüren mit rekombinationsstellen - Google Patents

Rekombinatorisches klonieren unter verwendung von nukleinsaüren mit rekombinationsstellen

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Publication number
ATE341621T1
ATE341621T1 AT98955110T AT98955110T ATE341621T1 AT E341621 T1 ATE341621 T1 AT E341621T1 AT 98955110 T AT98955110 T AT 98955110T AT 98955110 T AT98955110 T AT 98955110T AT E341621 T1 ATE341621 T1 AT E341621T1
Authority
AT
Austria
Prior art keywords
cloning
recombinatory
recombination site
nuclic acids
acids
Prior art date
Application number
AT98955110T
Other languages
English (en)
Inventor
James L Hartley
Michael A Brasch
Gary F Temple
Donna K Fox
Original Assignee
Invitrogen Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Invitrogen Corp filed Critical Invitrogen Corp
Application granted granted Critical
Publication of ATE341621T1 publication Critical patent/ATE341621T1/de

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Saccharide Compounds (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Gas Separation By Absorption (AREA)
AT98955110T 1997-10-24 1998-10-26 Rekombinatorisches klonieren unter verwendung von nukleinsaüren mit rekombinationsstellen ATE341621T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US6593097P 1997-10-24 1997-10-24
US17738798A 1998-10-23 1998-10-23

Publications (1)

Publication Number Publication Date
ATE341621T1 true ATE341621T1 (de) 2006-10-15

Family

ID=26746186

Family Applications (1)

Application Number Title Priority Date Filing Date
AT98955110T ATE341621T1 (de) 1997-10-24 1998-10-26 Rekombinatorisches klonieren unter verwendung von nukleinsaüren mit rekombinationsstellen

Country Status (11)

Country Link
US (15) US6277608B1 (de)
EP (1) EP1025217B1 (de)
JP (2) JP4303418B2 (de)
CN (1) CN100342008C (de)
AT (1) ATE341621T1 (de)
AU (1) AU752704B2 (de)
CA (1) CA2307016A1 (de)
DE (1) DE69836092T2 (de)
IL (1) IL135776A0 (de)
NZ (3) NZ520579A (de)
WO (1) WO1999021977A1 (de)

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CA2307016A1 (en) 1999-05-06
DE69836092T2 (de) 2007-05-10
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US20020172997A1 (en) 2002-11-21
WO1999021977A1 (en) 1999-05-06
HK1034287A1 (en) 2001-10-19
US20140004567A1 (en) 2014-01-02
US20090186385A1 (en) 2009-07-23
EP1025217A4 (de) 2002-11-13
EP1025217A1 (de) 2000-08-09
US20100267120A1 (en) 2010-10-21
DE69836092D1 (de) 2006-11-16
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US20040171157A1 (en) 2004-09-02
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US20040253631A1 (en) 2004-12-16
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US6277608B1 (en) 2001-08-21
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US20050009091A1 (en) 2005-01-13
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