ATE341621T1 - Rekombinatorisches klonieren unter verwendung von nukleinsaüren mit rekombinationsstellen - Google Patents

Rekombinatorisches klonieren unter verwendung von nukleinsaüren mit rekombinationsstellen

Info

Publication number
ATE341621T1
ATE341621T1 AT98955110T AT98955110T ATE341621T1 AT E341621 T1 ATE341621 T1 AT E341621T1 AT 98955110 T AT98955110 T AT 98955110T AT 98955110 T AT98955110 T AT 98955110T AT E341621 T1 ATE341621 T1 AT E341621T1
Authority
AT
Austria
Prior art keywords
cloning
recombinatory
recombination site
nuclic acids
acids
Prior art date
Application number
AT98955110T
Other languages
English (en)
Inventor
James L Hartley
Michael A Brasch
Gary F Temple
Donna K Fox
Original Assignee
Invitrogen Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Invitrogen Corp filed Critical Invitrogen Corp
Application granted granted Critical
Publication of ATE341621T1 publication Critical patent/ATE341621T1/de

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Saccharide Compounds (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Gas Separation By Absorption (AREA)
AT98955110T 1997-10-24 1998-10-26 Rekombinatorisches klonieren unter verwendung von nukleinsaüren mit rekombinationsstellen ATE341621T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US6593097P 1997-10-24 1997-10-24
US17738798A 1998-10-23 1998-10-23

Publications (1)

Publication Number Publication Date
ATE341621T1 true ATE341621T1 (de) 2006-10-15

Family

ID=26746186

Family Applications (1)

Application Number Title Priority Date Filing Date
AT98955110T ATE341621T1 (de) 1997-10-24 1998-10-26 Rekombinatorisches klonieren unter verwendung von nukleinsaüren mit rekombinationsstellen

Country Status (11)

Country Link
US (15) US6277608B1 (de)
EP (1) EP1025217B1 (de)
JP (2) JP4303418B2 (de)
CN (1) CN100342008C (de)
AT (1) ATE341621T1 (de)
AU (1) AU752704B2 (de)
CA (1) CA2307016A1 (de)
DE (1) DE69836092T2 (de)
IL (1) IL135776A0 (de)
NZ (3) NZ533288A (de)
WO (1) WO1999021977A1 (de)

Families Citing this family (308)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6720140B1 (en) 1995-06-07 2004-04-13 Invitrogen Corporation Recombinational cloning using engineered recombination sites
CA2226463A1 (en) 1995-06-07 1996-12-19 Life Technologies Corporation Recombinational cloning using engineered recombination sites
US6964861B1 (en) * 1998-11-13 2005-11-15 Invitrogen Corporation Enhanced in vitro recombinational cloning of using ribosomal proteins
US6143557A (en) * 1995-06-07 2000-11-07 Life Technologies, Inc. Recombination cloning using engineered recombination sites
US5851808A (en) * 1997-02-28 1998-12-22 Baylor College Of Medicine Rapid subcloning using site-specific recombination
US7227057B2 (en) 1997-06-03 2007-06-05 Chromatin, Inc. Plant centromere compositions
US7193128B2 (en) 1997-06-03 2007-03-20 Chromatin, Inc. Methods for generating or increasing revenues from crops
US7119250B2 (en) 1997-06-03 2006-10-10 The University Of Chicago Plant centromere compositions
US7235716B2 (en) 1997-06-03 2007-06-26 Chromatin, Inc. Plant centromere compositions
DE69836092T2 (de) * 1997-10-24 2007-05-10 Invitrogen Corp., Carlsbad Rekombinatorisches klonieren unter verwendung von nukleinsaüren mit rekombinationsstellen
CN101125873A (zh) * 1997-10-24 2008-02-20 茵维特罗根公司 利用具重组位点的核酸进行重组克隆
US7393632B2 (en) * 1999-12-10 2008-07-01 Invitrogen Corp. Use of multiple recombination sites with unique specificity in recombinational cloning
US7351578B2 (en) * 1999-12-10 2008-04-01 Invitrogen Corp. Use of multiple recombination sites with unique specificity in recombinational cloning
CA2327387C (en) 1998-05-07 2012-07-03 Gx Biosystems A/S Cytotoxin-based biological containment
WO2000005355A1 (en) * 1998-07-24 2000-02-03 Baylor College Of Medicine Rapid subcloning using site-specific recombination
EP1133523A1 (de) 1998-11-16 2001-09-19 Genway Biotech, Inc. Antikörpererzeugung durch polynukleotidimpfung von vögeln
DE60042969D1 (de) * 1999-03-02 2009-10-29 Life Technologies Corp Zubereitungen und methoden zur verwendung in rekombinatorischem klonen von nukleinsäuren
US7989202B1 (en) 1999-03-18 2011-08-02 The University Of Chicago Plant centromere compositions
US6709852B1 (en) * 1999-06-22 2004-03-23 Invitrogen Corporation Rapid growing microorganisms for biotechnology applications
DE19941186A1 (de) * 1999-08-30 2001-03-01 Peter Droege Sequenz-spezifische DNA-Rekombination in eukaryotischen Zellen
GB9921937D0 (en) * 1999-09-17 1999-11-17 Univ Leeds Targeted gens removal
CA2388907A1 (en) * 1999-10-25 2001-05-03 Life Technologies Corporation Methods of manipulating and sequencing nucleic acid molecules using transposition and recombination
US7244560B2 (en) 2000-05-21 2007-07-17 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
US6551828B1 (en) 2000-06-28 2003-04-22 Protemation, Inc. Compositions and methods for generating expression vectors through site-specific recombination
US7198924B2 (en) 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
JP4830079B2 (ja) * 2000-11-29 2011-12-07 国立感染症研究所長 プロテアーゼ活性の測定方法
AU2002227153B2 (en) 2000-12-08 2008-01-24 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
US7214515B2 (en) * 2001-01-05 2007-05-08 The General Hospital Corporation Viral delivery system for infectious transfer of large genomic DNA inserts
US8470580B2 (en) 2001-02-23 2013-06-25 Universite Libre De Bruxelles Method for the selection of recombination clones comprising a sequence encoding an antidote protein to a toxic molecule
JP2004531259A (ja) * 2001-04-19 2004-10-14 インヴィトロジェン コーポレーション 核酸分子の組換えクローニングのための組成物および方法
CA2448505A1 (en) * 2001-05-21 2002-11-28 Invitrogen Corporation Compositions and methods for use in isolation of nucleic acid molecules
CN1323170C (zh) * 2001-08-28 2007-06-27 日本烟草产业株式会社 修饰高等植物基因组的方法
AU2002356891A1 (en) * 2001-11-02 2003-05-19 Intradigm Corporation Method and system for inducible recombinational cloning in bacterial cells
JP4210769B2 (ja) * 2001-11-22 2009-01-21 独立行政法人産業技術総合研究所 Gatewayエントリークローンの作製方法
US7338800B2 (en) * 2002-01-16 2008-03-04 Baylor College Of Medicine In vivo gene transfer
US6818420B2 (en) 2002-02-27 2004-11-16 Biosource International, Inc. Methods of using FET labeled oligonucleotides that include a 3′-5′ exonuclease resistant quencher domain and compositions for practicing the same
US20040005710A1 (en) * 2002-03-09 2004-01-08 Ho-Sun Son Method for producing recombinant viruses using site-specific recombination
CA2480642C (en) 2002-03-29 2012-10-16 Janet Louise Suttie Lambda integrase mediated recombination in plants
AU2002257486A1 (en) * 2002-04-25 2003-11-10 Institute Of Zoology, Chinese Academy Of Sciences Method for constructing a linear expression vector without cloning
AU2003231244A1 (en) * 2002-05-02 2003-11-17 University Of North Carolina At Chapel Hill In vitro mutagenesis, phenotyping, and gene mapping
US20030235814A1 (en) * 2002-06-19 2003-12-25 Los Alamos National Laboratory Compositions and methods for selecting open reading frames
US20040132133A1 (en) * 2002-07-08 2004-07-08 Invitrogen Corporation Methods and compositions for the production, identification and purification of fusion proteins
AU2003253992A1 (en) * 2002-07-18 2004-02-09 Robert P. Bennett Viral vectors containing recombination sites
US9309518B2 (en) 2002-09-03 2016-04-12 Universite Libre De Bruxelles Reversible, parallel and multitask cloning method and kit
CN1703509B (zh) 2002-09-03 2010-09-01 布鲁塞尔自由大学 可逆的、平行的和多任务的克隆方法和试剂盒
US20040115642A1 (en) * 2002-12-12 2004-06-17 Changlin Fu Single-step methods for gene cloning
US7078234B2 (en) 2002-12-18 2006-07-18 Monsanto Technology Llc Maize embryo-specific promoter compositions and methods for use thereof
US20040214306A1 (en) * 2003-01-23 2004-10-28 Bloom Fredric R. Rapid growing microorganisms for biotechnology applications
EP1616949A4 (de) * 2003-04-07 2006-06-21 Jujo Paper Co Ltd Neuer vektor und verfahren zur konstruktion einer transformantenpflanze unter verwendung des vektors
EP1881074B1 (de) 2003-05-05 2013-01-02 Monsanto Technology, LLC HRGP Promotorkonstrukte
US7323313B2 (en) * 2003-05-09 2008-01-29 Health Research, Inc. Methods for protein interaction determination
WO2004104194A1 (en) * 2003-05-15 2004-12-02 Invitrogen Corporation Compositions and methods for site-specific recombination and expression
US20050095615A1 (en) * 2003-06-26 2005-05-05 Welch Peter J. Methods and compositions for detecting promoter activity and expressing fusion proteins
WO2005014796A2 (en) 2003-08-08 2005-02-17 Invitrogen Corporation Methods and compositions for seamless cloning of nucleic acid molecules
EP2272967B1 (de) 2003-10-02 2015-12-23 Monsanto Technology LLC Sammeln von Kulturpflanzenverbesserungsmerkmalen bei transgenen Pflanzen
WO2005035769A2 (en) 2003-10-09 2005-04-21 E. I. Du Pont De Nemours And Company Gene silencing by using micro-rna molecules
JP2007512838A (ja) 2003-12-01 2007-05-24 インヴィトロジェン コーポレーション 組換え部位を含む核酸分子およびその使用方法
US7935862B2 (en) 2003-12-02 2011-05-03 Syngenta Participations Ag Targeted integration and stacking of DNA through homologous recombination
US20050227309A1 (en) 2004-01-21 2005-10-13 Corry Schuyler B Optically-detectable enzyme substrates and their method of use
AR047598A1 (es) 2004-02-10 2006-01-25 Monsanto Technology Llc Semilla de maiz transgenica con mayor contenido de aminoacidos
EP1718754A1 (de) * 2004-02-23 2006-11-08 Chromatin, Inc. Mit minichromosomen modifizierte pflanzen
US20090098611A1 (en) * 2004-02-27 2009-04-16 Wood David W Self-cleaving affinity tags and methods of use
US20050282265A1 (en) * 2004-04-19 2005-12-22 Laura Vozza-Brown Electroporation apparatus and methods
BRPI0511046A (pt) * 2004-05-10 2007-11-27 Basf Plant Science Gmbh métodos para produzir uma construção de expressão múltipla, para produzir alimento ou produtos alimentìcios, produtos farmacêuticos ou produtos quìmicos, e uma planta ou célula de planta transgênica, construção de expressão múltipla, microorganismo,e, uso de um microorganismo, de uma célula de planta, de um tecido de planta, de um orgão de planta, ou de uma parte de uma planta
EP2484772B1 (de) 2004-05-18 2016-08-17 Intrexon Corporation Verfahren zur dynamischen Vektorassemblierung zur DNA-Klonierung von Vektorplasmiden
US20060064247A1 (en) * 2004-07-14 2006-03-23 Shao-Min Yuan Methods and systems for in silico experimental design and for providing a biotechnology product to a customer
JP2006141320A (ja) * 2004-11-22 2006-06-08 Invitrogen Japan Kk 複数の核酸断片をクローニングする方法
US8314290B2 (en) 2004-12-21 2012-11-20 Monsanto Technology Llc Temporal regulation of gene expression by MicroRNAs
WO2006073727A2 (en) 2004-12-21 2006-07-13 Monsanto Technology, Llc Recombinant dna constructs and methods for controlling gene expression
US20060200878A1 (en) 2004-12-21 2006-09-07 Linda Lutfiyya Recombinant DNA constructs and methods for controlling gene expression
CA2591992A1 (en) 2004-12-22 2006-06-29 The Salk Institute For Biological Studies Compositions and methods for producing recombinant proteins
US7582475B2 (en) 2005-01-07 2009-09-01 University Of Georgia Research Foundation, Inc. Vectors and methods for high throughput co-expression
CA2606220A1 (en) * 2005-04-19 2006-12-21 Basf Plant Science Gmbh Starchy-endosperm and/or germinating embryo-specific expression in mono-cotyledonous plants
USD564100S1 (en) 2005-04-19 2008-03-11 Invitrogen Corporation Electroporation vessel
ATE545701T1 (de) * 2005-05-17 2012-03-15 Ozgene Pty Ltd Sequentielles klonierungssystem
US20060270040A1 (en) * 2005-05-26 2006-11-30 Conjugon, Inc. Compositions and methods for treating tissue
EP2272968A3 (de) * 2005-09-08 2011-08-31 Chromatin, Inc. Mit Minichromosomen modifizierte Pflanzen
US9121028B2 (en) * 2005-09-09 2015-09-01 Monsanto Technology Llc Selective gene expression in plants
US20070117179A1 (en) * 2005-09-27 2007-05-24 Invitrogen Corporation In vitro protein synthesis systems for membrane proteins that include adolipoproteins and phospholipid-adolipoprotein particles
US20070243617A1 (en) * 2005-10-13 2007-10-18 Holt Robert A Modular genomes for synthetic biology and metabolic engineering
CA2625031C (en) 2005-10-13 2016-07-19 Monsanto Technology Llc Methods for producing hybrid seed
US20070231906A1 (en) * 2005-10-25 2007-10-04 Invitrogen Corporation Three Frame cDNA Expression Libraries for Functional Screening of Proteins
WO2007137300A2 (en) 2006-05-23 2007-11-29 Bellicum Pharmaceuticals, Inc. Modified dendritic cells having enhanced survival and immunogenicity and related compositions and methods
EP2059596B1 (de) 2006-08-31 2017-10-04 Monsanto Technology, LLC Srna in phasen
EP2664673A3 (de) 2006-10-05 2014-02-19 E. I. du Pont de Nemours and Company microRNA-Sequenzen von Mais
CN101802215A (zh) 2006-10-12 2010-08-11 孟山都技术有限公司 植物微rna及其使用方法
WO2008098181A2 (en) * 2007-02-09 2008-08-14 University Of Utah Research Foundation In vivo genome-wide mutagenesis
CA2675926A1 (en) 2007-02-16 2008-08-21 Basf Plant Science Gmbh Nucleic acid sequences for regulation of embryo-specific expression in monocotyledonous plants
WO2008103643A1 (en) 2007-02-20 2008-08-28 Monsanto Technology, Llc Invertebrate micrornas
EP2118129A4 (de) * 2007-03-01 2010-04-28 Life Technologies Corp Isolierte phospholipidproteinpartikel
WO2008112972A2 (en) * 2007-03-15 2008-09-18 Chromatin, Inc. Centromere sequences and minichromosomes
WO2009021215A1 (en) * 2007-08-09 2009-02-12 Celula, Inc. Methods and devices for correlated, multi-parameter single cell measurements and recovery of remnant biological material
US20090280103A1 (en) * 2008-04-04 2009-11-12 Martin Flueck Regulation of muscle repair
US9040774B2 (en) 2008-07-01 2015-05-26 Monsanto Technology Llc Recombinant DNA constructs encoding ribonuclease cleavage blockers and methods for modulating expression of a target gene
WO2011003034A2 (en) 2009-07-02 2011-01-06 Verdezyne, Inc. Biological methods for preparing adipic acid
AP3766A (en) 2009-07-09 2016-07-31 Du Pont Engineered microorganisms with enhanced fermentation activity
WO2011011693A1 (en) 2009-07-23 2011-01-27 Chromatin, Inc. Sorghum centromere sequences and minichromosomes
US8889394B2 (en) * 2009-09-07 2014-11-18 Empire Technology Development Llc Multiple domain proteins
WO2011105429A1 (ja) * 2010-02-24 2011-09-01 学校法人日本大学 部位特異的組換え方法及びキット
CN101967475B (zh) * 2010-06-10 2014-01-22 复旦大学 一种基于位点特异性重组的基因打靶载体快速构建方法
CN101921747A (zh) * 2010-06-10 2010-12-22 复旦大学 一种基于位点特异性重组的克隆方法
DE102010056289A1 (de) 2010-12-24 2012-06-28 Geneart Ag Verfahren zur Herstellung von Leseraster-korrekten Fragment-Bibliotheken
JP2011078436A (ja) * 2011-01-27 2011-04-21 National Institute Of Infectious Diseases プロテアーゼ活性の測定方法
US8343752B2 (en) 2011-05-03 2013-01-01 Verdezyne, Inc. Biological methods for preparing adipic acid
US8728798B2 (en) 2011-05-03 2014-05-20 Verdezyne, Inc. Biological methods for preparing adipic acid
CA2841796C (en) 2011-07-06 2021-06-29 Verdezyne, Inc. Biological methods for preparing a fatty dicarboxylic acid
EP2795501A2 (de) 2011-12-21 2014-10-29 Life Technologies Corporation Verfahren und systeme für experimentelles in-silico-designing und zur ausführung eines biologischen workflows
WO2013152220A2 (en) 2012-04-04 2013-10-10 Life Technologies Corporation Tal-effector assembly platform, customized services, kits and assays
WO2014093701A1 (en) 2012-12-12 2014-06-19 The Broad Institute, Inc. Functional genomics using crispr-cas systems, compositions, methods, knock out libraries and applications thereof
DK2921557T3 (en) 2012-12-12 2016-11-07 Broad Inst Inc Design of systems, methods and optimized sequence manipulation guide compositions
IL300461A (en) 2012-12-12 2023-04-01 Harvard College Engineering and optimization of improved systems, methods and enzyme compositions for sequence manipulation
DK3327127T3 (da) 2012-12-12 2021-06-28 Broad Inst Inc Fremføring, modificering og optimering af systemer, fremgangsmåder og sammensætninger til sekvensmanipulation og terapeutiske anvendelser
HK1216431A1 (zh) 2012-12-19 2016-11-11 科威生物制品有限公司 制备脂肪二羧酸的生物学方法
WO2014100504A2 (en) 2012-12-19 2014-06-26 Verdezyne, Inc. Biological methods for preparing a fatty dicarboxylic acid
CA2915795C (en) 2013-06-17 2021-07-13 The Broad Institute, Inc. Delivery, use and therapeutic applications of the crispr-cas systems and compositions for targeting disorders and diseases using viral components
AU2014281028B2 (en) 2013-06-17 2020-09-10 Massachusetts Institute Of Technology Delivery and use of the CRISPR-Cas systems, vectors and compositions for hepatic targeting and therapy
JP6665088B2 (ja) 2013-06-17 2020-03-13 ザ・ブロード・インスティテュート・インコーポレイテッド 配列操作のための最適化されたCRISPR−Cas二重ニッカーゼ系、方法および組成物
AU2014281030B2 (en) 2013-06-17 2020-07-09 Massachusetts Institute Of Technology Delivery, engineering and optimization of systems, methods and compositions for targeting and modeling diseases and disorders of post mitotic cells
WO2014204724A1 (en) 2013-06-17 2014-12-24 The Broad Institute Inc. Delivery, engineering and optimization of tandem guide systems, methods and compositions for sequence manipulation
CN110564663B (zh) 2013-08-19 2023-03-10 辛格隆股份公司 微生物的受控生长
WO2015089473A1 (en) 2013-12-12 2015-06-18 The Broad Institute Inc. Engineering of systems, methods and optimized guide compositions with new architectures for sequence manipulation
SG10201804974RA (en) 2013-12-12 2018-07-30 Broad Inst Inc Compositions and Methods of Use of Crispr-Cas Systems in Nucleotide Repeat Disorders
AU2014361826A1 (en) 2013-12-12 2016-06-23 Massachusetts Institute Of Technology Delivery, use and therapeutic applications of the CRISPR-Cas systems and compositions for targeting disorders and diseases using particle delivery components
IL289736B2 (en) 2013-12-12 2025-09-01 Massachusetts Inst Technology Administration, use and therapeutic applications of CRISPR–Cas gene editing systems and gene editing preparations
AU2014361784A1 (en) 2013-12-12 2016-06-23 Massachusetts Institute Of Technology Delivery, use and therapeutic applications of the CRISPR-Cas systems and compositions for HBV and viral diseases and disorders
WO2015089364A1 (en) 2013-12-12 2015-06-18 The Broad Institute Inc. Crystal structure of a crispr-cas system, and uses thereof
HUE056155T2 (hu) 2014-04-09 2022-01-28 Scripps Research Inst Nem-természetes vagy módosított nukleozid-trifoszfátok sejtekbe importálása nukleinsav-trifoszfát transzporterekkel
EP3180426B1 (de) 2014-08-17 2019-12-25 The Broad Institute, Inc. Gemonbearbeitung mit cas9-nickasen
WO2016049024A2 (en) 2014-09-24 2016-03-31 The Broad Institute Inc. Delivery, use and therapeutic applications of the crispr-cas systems and compositions for modeling competition of multiple cancer mutations in vivo
WO2016049251A1 (en) 2014-09-24 2016-03-31 The Broad Institute Inc. Delivery, use and therapeutic applications of the crispr-cas systems and compositions for modeling mutations in leukocytes
WO2016049163A2 (en) 2014-09-24 2016-03-31 The Broad Institute Inc. Use and production of chd8+/- transgenic animals with behavioral phenotypes characteristic of autism spectrum disorder
CN106715691B (zh) * 2014-10-14 2020-06-02 深圳华大智造科技有限公司 一种转座酶打断核酸并加接头的方法和试剂
US20170247762A1 (en) 2014-10-27 2017-08-31 The Board Institute Inc. Compositions, methods and use of synthetic lethal screening
FR3028527A1 (fr) 2014-11-13 2016-05-20 Pivert Identification de facteurs de transcription de yarrowia lipolytica
WO2016094867A1 (en) 2014-12-12 2016-06-16 The Broad Institute Inc. Protected guide rnas (pgrnas)
WO2016094874A1 (en) 2014-12-12 2016-06-16 The Broad Institute Inc. Escorted and functionalized guides for crispr-cas systems
WO2016094872A1 (en) 2014-12-12 2016-06-16 The Broad Institute Inc. Dead guides for crispr transcription factors
WO2016100974A1 (en) 2014-12-19 2016-06-23 The Broad Institute Inc. Unbiased identification of double-strand breaks and genomic rearrangement by genome-wide insert capture sequencing
WO2016106236A1 (en) 2014-12-23 2016-06-30 The Broad Institute Inc. Rna-targeting system
AU2015369725A1 (en) 2014-12-24 2017-06-29 Massachusetts Institute Of Technology CRISPR having or associated with destabilization domains
WO2016108926A1 (en) 2014-12-30 2016-07-07 The Broad Institute Inc. Crispr mediated in vivo modeling and genetic screening of tumor growth and metastasis
US20180148744A1 (en) 2015-03-20 2018-05-31 Verdezyne, Inc. Biological methods for preparing 3-hydroxypropionic acid
WO2016205728A1 (en) 2015-06-17 2016-12-22 Massachusetts Institute Of Technology Crispr mediated recording of cellular events
FI3430134T3 (fi) 2015-06-18 2023-01-13 Uusia CRISPR-entsyymejä ja järjestelmiä
WO2016205745A2 (en) 2015-06-18 2016-12-22 The Broad Institute Inc. Cell sorting
WO2016205749A1 (en) 2015-06-18 2016-12-22 The Broad Institute Inc. Novel crispr enzymes and systems
US9790490B2 (en) 2015-06-18 2017-10-17 The Broad Institute Inc. CRISPR enzymes and systems
IL293323B2 (en) 2015-06-18 2024-01-01 Massachusetts Inst Technology Crispr enzyme mutations reducing off-target effects
US12241053B2 (en) 2015-10-09 2025-03-04 The Brigham And Women's Hospital, Inc. Modulation of novel immune checkpoint targets
CN109153980B (zh) 2015-10-22 2023-04-14 布罗德研究所有限公司 Vi-b型crispr酶和系统
EP3368687B1 (de) 2015-10-27 2021-09-29 The Broad Institute, Inc. Zusammensetzungen und verfahren gegen krebsspezifische sequenzvariationen
WO2017075451A1 (en) 2015-10-28 2017-05-04 The Broad Institute Inc. Compositions and methods for evaluating and modulating immune responses by detecting and targeting pou2af1
WO2017075465A1 (en) 2015-10-28 2017-05-04 The Broad Institute Inc. Compositions and methods for evaluating and modulating immune responses by detecting and targeting gata3
WO2017075478A2 (en) 2015-10-28 2017-05-04 The Broad Institute Inc. Compositions and methods for evaluating and modulating immune responses by use of immune cell gene signatures
US12110490B2 (en) 2015-12-18 2024-10-08 The Broad Institute, Inc. CRISPR enzymes and systems
WO2017106767A1 (en) 2015-12-18 2017-06-22 The Scripps Research Institute Production of unnatural nucleotides using a crispr/cas9 system
EP3445853A1 (de) 2016-04-19 2019-02-27 The Broad Institute, Inc. Cpf1-komplexe mit reduzierter indelaktivität
AU2017253089B2 (en) 2016-04-19 2023-07-20 Massachusetts Institute Of Technology Novel CRISPR enzymes and systems
EP3445856A1 (de) 2016-04-19 2019-02-27 The Broad Institute Inc. Neuartige crispr-enzyme und -systeme
US11939571B2 (en) 2016-06-10 2024-03-26 President And Fellows Of Harvard College Library-scale engineering of metabolic pathways
KR20190019168A (ko) 2016-06-17 2019-02-26 더 브로드 인스티튜트, 인코퍼레이티드 제vi형 crispr 오솔로그 및 시스템
EP4163293A1 (de) 2016-06-24 2023-04-12 The Scripps Research Institute Neuartiger nukleosid-triphosphat-transporter und verwendungen davon
WO2018005873A1 (en) 2016-06-29 2018-01-04 The Broad Institute Inc. Crispr-cas systems having destabilization domain
US12431216B2 (en) 2016-08-17 2025-09-30 Broad Institute, Inc. Methods for identifying class 2 crispr-cas systems
US20200283743A1 (en) 2016-08-17 2020-09-10 The Broad Institute, Inc. Novel crispr enzymes and systems
US11352647B2 (en) 2016-08-17 2022-06-07 The Broad Institute, Inc. Crispr enzymes and systems
US20190262399A1 (en) 2016-09-07 2019-08-29 The Broad Institute, Inc. Compositions and methods for evaluating and modulating immune responses
US12499971B2 (en) 2016-09-28 2025-12-16 The Broad Institute, Inc. Systematic screening and mapping of regulatory elements in non-coding genomic regions, methods, compositions, and applications thereof
WO2018067991A1 (en) 2016-10-07 2018-04-12 The Brigham And Women's Hospital, Inc. Modulation of novel immune checkpoint targets
WO2018074979A1 (en) 2016-10-17 2018-04-26 Nanyang Technological University Truncated crispr-cas proteins for dna targeting
US10496048B2 (en) * 2016-11-02 2019-12-03 Edison Labs, Inc. Switch terminal methods with wiring components secured to circuitry wiring without external live points of contact
US10241477B2 (en) * 2016-11-02 2019-03-26 Edison Labs, Inc. Adaptive control methods for buildings with redundant circuitry
CN108665933B (zh) * 2016-11-02 2020-10-16 旺宏电子股份有限公司 非易失性存储器元件的操作方法及其应用
PT3551753T (pt) 2016-12-09 2022-09-02 Harvard College Diagnósticos baseados num sistema efetor de crispr
US12404514B2 (en) 2016-12-09 2025-09-02 The Broad Institute, Inc. CRISPR-systems for modifying a trait of interest in a plant
US11739308B2 (en) 2017-03-15 2023-08-29 The Broad Institute, Inc. Cas13b orthologues CRISPR enzymes and systems
EP3596218B1 (de) 2017-03-15 2023-08-23 The Broad Institute, Inc. Auf crispr-effektorsystem basierende diagnostik für virusnachweis
US11174515B2 (en) 2017-03-15 2021-11-16 The Broad Institute, Inc. CRISPR effector system based diagnostics
CN108624516B (zh) * 2017-03-20 2022-08-26 华东理工大学 一种提高发酵细胞中的代谢产物量及制备idms标准品的方法
WO2018175924A1 (en) 2017-03-24 2018-09-27 The Broad Institute, Inc. Methods and compositions for regulating innate lymphoid cell inflammatory responses
WO2018183908A1 (en) 2017-03-31 2018-10-04 Dana-Farber Cancer Institute, Inc. Compositions and methods for treating ovarian tumors
EP3610009A1 (de) 2017-04-12 2020-02-19 The Broad Institute, Inc. Neuartige typ-vi-crispr-orthologe und systeme
US20200071773A1 (en) 2017-04-12 2020-03-05 Massachusetts Eye And Ear Infirmary Tumor signature for metastasis, compositions of matter methods of use thereof
US11591601B2 (en) 2017-05-05 2023-02-28 The Broad Institute, Inc. Methods for identification and modification of lncRNA associated with target genotypes and phenotypes
US20210278416A1 (en) 2017-05-09 2021-09-09 The Broad Institute, Inc. Gut microbiome function predicts response to anti-integrin biologic therapy in inflammatory bowel diseases
EP3622092A4 (de) 2017-05-11 2021-06-23 The Broad Institute, Inc. Verfahren und zusammensetzungen zur verwendung von cd8+-tumor-infiltrierenden lymphozyten-subtypen und gensignaturen davon
WO2018213726A1 (en) 2017-05-18 2018-11-22 The Broad Institute, Inc. Systems, methods, and compositions for targeted nucleic acid editing
US11897953B2 (en) 2017-06-14 2024-02-13 The Broad Institute, Inc. Compositions and methods targeting complement component 3 for inhibiting tumor growth
US10011849B1 (en) 2017-06-23 2018-07-03 Inscripta, Inc. Nucleic acid-guided nucleases
AU2018289077B2 (en) 2017-06-23 2022-03-10 Inscripta, Inc. Nucleic acid-guided nucleases
US9982279B1 (en) 2017-06-23 2018-05-29 Inscripta, Inc. Nucleic acid-guided nucleases
AU2018290843B2 (en) 2017-06-26 2025-04-24 Massachusetts Institute Of Technology CRISPR/Cas-adenine deaminase based compositions, systems, and methods for targeted nucleic acid editing
SI3645719T1 (sl) 2017-06-30 2022-07-29 Inscripta, Inc., Postopki, moduli, instrumenti in sistemi za avtomatizirano obdelavo celic
CA3069321A1 (en) 2017-07-11 2019-01-17 The Scripps Research Institute Incorporation of unnatural nucleotides and methods thereof
US11174488B2 (en) 2017-07-13 2021-11-16 Radici Chimica S.P.A. Biological methods for modifying cellular carbon flux
WO2019014581A1 (en) 2017-07-14 2019-01-17 The Broad Institute, Inc. METHODS AND COMPOSITIONS FOR MODULATING THE ACTIVITY OF A CYTOTOXIC LYMPHOCYTE
EP3654993A4 (de) 2017-07-17 2021-08-25 The Broad Institute, Inc. Zellatlas des gesunden menschlichen dickdarms und menschlichen dickdarms mit colitis ulcerosa
KR20250145137A (ko) 2017-08-03 2025-10-13 신톡스, 인크. 자가면역 질환의 치료를 위한 사이토카인 접합체
US10738327B2 (en) 2017-08-28 2020-08-11 Inscripta, Inc. Electroporation cuvettes for automation
WO2019060746A1 (en) 2017-09-21 2019-03-28 The Broad Institute, Inc. SYSTEMS, METHODS, AND COMPOSITIONS FOR THE TARGETED EDITING OF NUCLEIC ACIDS
AU2018338990B2 (en) 2017-09-30 2022-06-23 Inscripta, Inc. Flow through electroporation instrumentation
EP3692152B1 (de) 2017-10-04 2025-09-03 The Broad Institute, Inc. Verfahren und zusammensetzungen zur veränderung der funktion und struktur von chromatinschleifen und/oder -domänen
US11547614B2 (en) 2017-10-31 2023-01-10 The Broad Institute, Inc. Methods and compositions for studying cell evolution
EP3710039A4 (de) 2017-11-13 2021-08-04 The Broad Institute, Inc. Verfahren und zusammensetzungen zur behandlung von krebs durch targeting des clec2d-klrb1-wegs
WO2019094984A1 (en) 2017-11-13 2019-05-16 The Broad Institute, Inc. Methods for determining spatial and temporal gene expression dynamics during adult neurogenesis in single cells
WO2019094955A1 (en) 2017-11-13 2019-05-16 The Broad Institute, Inc. Methods and compositions for targeting developmental and oncogenic programs in h3k27m gliomas
US11332736B2 (en) 2017-12-07 2022-05-17 The Broad Institute, Inc. Methods and compositions for multiplexing single cell and single nuclei sequencing
KR102777327B1 (ko) 2017-12-19 2025-03-05 신굴론 에스.에이. 발효 공정
WO2019126709A1 (en) 2017-12-22 2019-06-27 The Broad Institute, Inc. Cas12b systems, methods, and compositions for targeted dna base editing
CN112105627B (zh) 2017-12-29 2024-07-02 斯克利普斯研究所 非天然碱基对组合物及使用方法
US11994512B2 (en) 2018-01-04 2024-05-28 Massachusetts Institute Of Technology Single-cell genomic methods to generate ex vivo cell systems that recapitulate in vivo biology with improved fidelity
DK3746568T5 (da) 2018-01-29 2024-08-26 Broad Inst Inc Crispr-effektorsystembaseret diagnostik
EP3775159A4 (de) 2018-03-29 2022-01-19 Inscripta, Inc. Automatisierte steuerung von zellwachstumsgeschwindigkeiten für induktion und transformation
US10968257B2 (en) 2018-04-03 2021-04-06 The Broad Institute, Inc. Target recognition motifs and uses thereof
US10376889B1 (en) 2018-04-13 2019-08-13 Inscripta, Inc. Automated cell processing instruments comprising reagent cartridges
US10557216B2 (en) 2018-04-24 2020-02-11 Inscripta, Inc. Automated instrumentation for production of T-cell receptor peptide libraries
US10501738B2 (en) 2018-04-24 2019-12-10 Inscripta, Inc. Automated instrumentation for production of peptide libraries
US10858761B2 (en) 2018-04-24 2020-12-08 Inscripta, Inc. Nucleic acid-guided editing of exogenous polynucleotides in heterologous cells
US11957695B2 (en) 2018-04-26 2024-04-16 The Broad Institute, Inc. Methods and compositions targeting glucocorticoid signaling for modulating immune responses
WO2019210268A2 (en) 2018-04-27 2019-10-31 The Broad Institute, Inc. Sequencing-based proteomics
WO2019232542A2 (en) 2018-06-01 2019-12-05 Massachusetts Institute Of Technology Methods and compositions for detecting and modulating microenvironment gene signatures from the csf of metastasis patients
US12036240B2 (en) 2018-06-14 2024-07-16 The Broad Institute, Inc. Compositions and methods targeting complement component 3 for inhibiting tumor growth
WO2020006067A1 (en) 2018-06-26 2020-01-02 The Broad Institute, Inc. Crispr double nickase based amplification compositions, systems, and methods
AU2019292919A1 (en) 2018-06-30 2021-03-11 Inscripta, Inc. Instruments, modules, and methods for improved detection of edited sequences in live cells
US20210163944A1 (en) 2018-08-07 2021-06-03 The Broad Institute, Inc. Novel cas12b enzymes and systems
US20210308171A1 (en) * 2018-08-07 2021-10-07 The Broad Institute, Inc. Methods for combinatorial screening and use of therapeutic targets thereof
US10752874B2 (en) 2018-08-14 2020-08-25 Inscripta, Inc. Instruments, modules, and methods for improved detection of edited sequences in live cells
US10532324B1 (en) 2018-08-14 2020-01-14 Inscripta, Inc. Instruments, modules, and methods for improved detection of edited sequences in live cells
US11142740B2 (en) 2018-08-14 2021-10-12 Inscripta, Inc. Detection of nuclease edited sequences in automated modules and instruments
WO2020041387A1 (en) 2018-08-20 2020-02-27 The Brigham And Women's Hospital, Inc. Degradation domain modifications for spatio-temporal control of rna-guided nucleases
US12460244B2 (en) 2018-08-20 2025-11-04 The Broad Institute, Inc. Inhibitors of RNA-guided nuclease activity and uses thereof
WO2020041380A1 (en) 2018-08-20 2020-02-27 The Broad Institute, Inc. Methods and compositions for optochemical control of crispr-cas9
AU2019363487A1 (en) 2018-08-30 2021-04-15 Inscripta, Inc. Improved detection of nuclease edited sequences in automated modules and instruments
WO2020060948A1 (en) 2018-09-17 2020-03-26 Levadura Biotechnology, Inc. Production of cannabinoids in yeast using a fatty acid feedstock
EP3852813A4 (de) 2018-09-18 2022-06-22 VNV Newco Inc. Lichtbogenbasierte kapside und deren verwendungen
US12467925B2 (en) 2018-10-03 2025-11-11 The Broad Institute, Inc. CRISPR effector system based diagnostics for hemorrhagic fever detection
WO2020077135A1 (en) 2018-10-10 2020-04-16 Dana-Farber Cancer Institute, Inc. Modulating resistance to bcl-2 inhibitors
US20220411783A1 (en) 2018-10-12 2022-12-29 The Broad Institute, Inc. Method for extracting nuclei or whole cells from formalin-fixed paraffin-embedded tissues
CN112912392B (zh) 2018-10-26 2024-08-02 豪夫迈·罗氏有限公司 利用重组酶介导的盒式交换的多特异性抗体筛选方法
US12165743B2 (en) 2018-11-09 2024-12-10 The Broad Institute, Inc. Compressed sensing for screening and tissue imaging
US12402610B2 (en) 2018-11-09 2025-09-02 The Broad Institute, Inc. Methods and compositions for modulating innate lymphoid cell pathogenic effectors
EP3880844A2 (de) 2018-11-14 2021-09-22 President And Fellows Of Harvard College Multiplexen von sich hochentwickelnden viralen varianten mit sherlock-nachweismethode
KR20210104698A (ko) 2018-11-14 2021-08-25 더 브로드 인스티튜트, 인코퍼레이티드 Crispr 시스템 기반 액적 진단 시스템 및 방법
US20250043366A1 (en) 2018-12-13 2025-02-06 The Broad Institute, Inc. Tiled assays using crispr-cas based detection
CA3124110A1 (en) 2018-12-17 2020-06-25 The Broad Institute, Inc. Crispr-associated transposase systems and methods of use thereof
US11739156B2 (en) 2019-01-06 2023-08-29 The Broad Institute, Inc. Massachusetts Institute of Technology Methods and compositions for overcoming immunosuppression
US20220090091A1 (en) * 2019-01-09 2022-03-24 Asimov, Inc. Compositions of polynucleic acid vectors and uses thereof
SG11202107354WA (en) 2019-02-06 2021-08-30 Synthorx Inc Il-2 conjugates and methods of use thereof
US12215382B2 (en) 2019-03-01 2025-02-04 The General Hospital Corporation Liver protective MARC variants and uses thereof
WO2020186231A2 (en) 2019-03-14 2020-09-17 The Broad Institute, Inc. Crispr effector system based multiplex diagnostics
US12534714B2 (en) 2019-03-18 2026-01-27 The Broad Institute, Inc. Type VII CRISPR proteins and systems
EP3947437A1 (de) 2019-03-29 2022-02-09 F. Hoffmann-La Roche AG Verfahren zur erzeugung einer fcrn-exprimierenden zelle durch gezielte integration mehrerer expressionskassetten in einer definierten organisation
US20220175848A1 (en) 2019-04-01 2022-06-09 The Broad Institute, Inc. Methods and compositions for cell therapy
US20220249701A1 (en) 2019-05-14 2022-08-11 The Broad Institute, Inc. Compositions and methods for targeting multinucleated cells
US20220220469A1 (en) 2019-05-20 2022-07-14 The Broad Institute, Inc. Non-class i multi-component nucleic acid targeting systems
US20220243178A1 (en) 2019-05-31 2022-08-04 The Broad Institute, Inc. Methods for treating metabolic disorders by targeting adcy5
TWI874408B (zh) 2019-06-14 2025-03-01 美商史基普研究協會 於半合成生物體中複製、轉錄及轉譯之試劑及方法
US10907125B2 (en) 2019-06-20 2021-02-02 Inscripta, Inc. Flow through electroporation modules and instrumentation
CN114008070A (zh) 2019-06-21 2022-02-01 因思科瑞普特公司 导致大肠杆菌赖氨酸产量增加的全基因组合理设计的突变
US10927385B2 (en) 2019-06-25 2021-02-23 Inscripta, Inc. Increased nucleic-acid guided cell editing in yeast
US20220333208A1 (en) 2019-09-03 2022-10-20 The Broad Institute, Inc. Crispr effector system based multiplex cancer diagnostics
JP2022547570A (ja) 2019-09-12 2022-11-14 ザ・ブロード・インスティテュート・インコーポレイテッド 操作済みのアデノ随伴ウイルスキャプシド
US20220389451A1 (en) 2019-09-20 2022-12-08 The Broad Institute, Inc. Compositions and methods for delivering cargo to a target cell
US12297426B2 (en) 2019-10-01 2025-05-13 The Broad Institute, Inc. DNA damage response signature guided rational design of CRISPR-based systems and therapies
US11981922B2 (en) 2019-10-03 2024-05-14 Dana-Farber Cancer Institute, Inc. Methods and compositions for the modulation of cell interactions and signaling in the tumor microenvironment
US10689669B1 (en) 2020-01-11 2020-06-23 Inscripta, Inc. Automated multi-module cell processing methods, instruments, and systems
US12165747B2 (en) 2020-01-23 2024-12-10 The Broad Institute, Inc. Molecular spatial mapping of metastatic tumor microenvironment
CN115135370A (zh) 2020-01-27 2022-09-30 因思科瑞普特公司 电穿孔模块和仪器
US20210332388A1 (en) 2020-04-24 2021-10-28 Inscripta, Inc. Compositions, methods, modules and instruments for automated nucleic acid-guided nuclease editing in mammalian cells
US11787841B2 (en) 2020-05-19 2023-10-17 Inscripta, Inc. Rationally-designed mutations to the thrA gene for enhanced lysine production in E. coli
WO2022136370A1 (en) 2020-12-22 2022-06-30 Helmholtz Zentrum Muenchen - Deutsches Forschungszentrum Für Gesundheit Und Umwelt (Gmbh) Application of crispr/cas13 for therapy of rna virus and/or bacterium induced diseases
WO2023004367A2 (en) 2021-07-20 2023-01-26 The Broad Institute, Inc. Engineered targeting compositions for endothelial cells of the central nervous system vasculature and methods of use thereof
US20260022386A1 (en) 2021-11-01 2026-01-22 Basecamp Research Ltd Single Construct Platform for Simultaneous Delivery of Gene Editing Machinery and Nucleic Acid Cargo
WO2023122764A1 (en) 2021-12-22 2023-06-29 Tome Biosciences, Inc. Co-delivery of a gene editor construct and a donor template
WO2023196818A1 (en) 2022-04-04 2023-10-12 The Regents Of The University Of California Genetic complementation compositions and methods
WO2023200770A1 (en) 2022-04-12 2023-10-19 Inscripta, Inc. Curing for iterative nucleic acid-guided nuclease editing
WO2023205744A1 (en) 2022-04-20 2023-10-26 Tome Biosciences, Inc. Programmable gene insertion compositions
WO2023215831A1 (en) 2022-05-04 2023-11-09 Tome Biosciences, Inc. Guide rna compositions for programmable gene insertion
WO2023225564A1 (en) 2022-05-18 2023-11-23 The Broad Institute, Inc. Engineered viral capsids with increased stability and methods of use thereof
WO2023225670A2 (en) 2022-05-20 2023-11-23 Tome Biosciences, Inc. Ex vivo programmable gene insertion
JP2025524656A (ja) 2022-07-14 2025-07-30 ザ・ブロード・インスティテュート・インコーポレイテッド トランスフェリン受容体との相互作用を通じてcns全体への遺伝子送達を可能にするaavキャプシド
WO2024020587A2 (en) 2022-07-22 2024-01-25 Tome Biosciences, Inc. Pleiopluripotent stem cell programmable gene insertion
CN116024234B (zh) * 2022-12-12 2023-07-21 南京林业大学 一种杨树壳针孢菌效应蛋白SmCSEP3及其应用
WO2024138194A1 (en) 2022-12-22 2024-06-27 Tome Biosciences, Inc. Platforms, compositions, and methods for in vivo programmable gene insertion
WO2024163842A2 (en) 2023-02-03 2024-08-08 The Broad Institute, Inc. Delivering genes to the brain endothelium to treat lysosomal storage disorder-derived neuropathology
WO2024168253A1 (en) 2023-02-10 2024-08-15 Possible Medicines Llc Delivery of an rna guided recombination system
WO2024168265A1 (en) 2023-02-10 2024-08-15 Possible Medicines Llc Aav delivery of rna guided recombination system
WO2024234006A1 (en) 2023-05-11 2024-11-14 Tome Biosciences, Inc. Systems, compositions, and methods for targeting liver sinusodial endothelial cells (lsecs)
WO2024238666A1 (en) 2023-05-16 2024-11-21 Inscripta, Inc. Methods to enable insertion of large fragments of dna into genomes of live cells at library scale
WO2025050069A1 (en) 2023-09-01 2025-03-06 Tome Biosciences, Inc. Programmable gene insertion using engineered integration enzymes
WO2025072383A1 (en) 2023-09-25 2025-04-03 The Broad Institute, Inc. Viral open reading frames, uses thereof, and methods of detecting the same
WO2025097055A2 (en) 2023-11-02 2025-05-08 The Broad Institute, Inc. Compositions and methods of use of t cells in immunotherapy
WO2025129158A1 (en) 2023-12-15 2025-06-19 The Broad Institute, Inc. Engineered arc delivery vesicles and uses thereof
WO2025155923A1 (en) 2024-01-17 2025-07-24 The Broad Institute, Inc. Aav capsid modifications that enable improved cns-wide gene delivery through interactions with the transferrin receptor
WO2025160155A1 (en) 2024-01-22 2025-07-31 The Broad Institute, Inc. Epigenetic targeting of prion diseases
US20250276095A1 (en) 2024-03-04 2025-09-04 Kate Therapeutics, Inc. Adeno-associated virus compositions for the treatment of duchenne muscular dystrophy
WO2025217163A2 (en) 2024-04-08 2025-10-16 The Broad Institute, Inc. Novel aav capsids binding to human cd59
WO2025217174A1 (en) 2024-04-08 2025-10-16 The Broad Institute, Inc. Aav capsid modifications that enable improved cns-wide gene delivery through interactions with carbonic anhydrase iv
WO2025224107A1 (en) 2024-04-22 2025-10-30 Basecamp Research Ltd Method and compositions for detecting off-target editing
WO2025224182A2 (en) 2024-04-23 2025-10-30 Basecamp Research Ltd Single construct platform for simultaneous delivery of gene editing machinery and nucleic acid cargo
WO2025226841A1 (en) 2024-04-24 2025-10-30 Kate Therapeutics, Inc. Gene therapy approach for treating disorders associated with tnnt2
WO2025226842A1 (en) 2024-04-24 2025-10-30 Kate Therapeutics, Inc. Expression control by drg-expressed mirnas
WO2025250808A1 (en) 2024-05-29 2025-12-04 The Brigham And Women’S Hospital, Inc. Anti-crispr delivery compositions and methods
WO2026011009A1 (en) 2024-07-02 2026-01-08 Kate Therapeutics, Inc. Compositions and methods for muscle disorders
WO2026011008A1 (en) 2024-07-02 2026-01-08 Kate Therapeutics, Inc. Expression control by skeletal muscle-expressed mirnas
WO2026030242A1 (en) 2024-07-29 2026-02-05 Kate Therapeutics, Inc. Adeno-associated virus compositions for the treatment of facioscapulohumeral muscular dystrophy
WO2026030244A1 (en) 2024-07-30 2026-02-05 Kate Therapeutics, Inc. Adeno-associated virus compositions for the treatment of limb girdle muscular dystrophy 2a
WO2026050402A1 (en) 2024-08-29 2026-03-05 Kate Therapeutics, Inc. Liver de-targeted muscle tropic capsids

Family Cites Families (238)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5844072A (en) 1992-05-26 1998-12-01 University Of California Antibiotic cryptdin peptides and methods of their use
US4372745A (en) * 1979-12-19 1983-02-08 Electro-Nucleonics, Inc. Chemical luminescence amplification substrate system for immunochemistry involving microencapsulated fluorescer
US5097097A (en) * 1980-07-30 1992-03-17 Abbott Laboratories Triazinylaminofluoresceins
US4626505A (en) * 1984-02-24 1986-12-02 E. I. Du Pont De Nemours And Company Selectable markers for yeast transformation
US4673640A (en) 1984-04-30 1987-06-16 Biotechnica International, Inc. Regulated protein production using site-specific recombination
US4855231A (en) * 1984-10-30 1989-08-08 Phillips Petroleum Company Regulatory region for heterologous gene expression in yeast
US4808537A (en) * 1984-10-30 1989-02-28 Phillips Petroleum Company Methanol inducible genes obtained from pichia and methods of use
US4743546A (en) 1985-02-13 1988-05-10 Biotechnica International, Inc. Controlled gene excision
US4797368A (en) * 1985-03-15 1989-01-10 The United States Of America As Represented By The Department Of Health And Human Services Adeno-associated virus as eukaryotic expression vector
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4965188A (en) * 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
ATE93541T1 (de) * 1985-07-03 1993-09-15 Genencor Int Hybride polypeptide und verfahren zu deren herstellung.
US6121043A (en) * 1985-09-06 2000-09-19 Syntro Corporation Recombinant herpesvirus of turkeys comprising a foreign DNA inserted into a non-essential region of the herpesvirus of turkeys genome
CA1293460C (en) * 1985-10-07 1991-12-24 Brian Lee Sauer Site-specific recombination of dna in yeast
US4800159A (en) * 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
US4959217A (en) * 1986-05-22 1990-09-25 Syntex (U.S.A.) Inc. Delayed/sustained release of macromolecules
US5079352A (en) * 1986-08-22 1992-01-07 Cetus Corporation Purified thermostable enzyme
US4987071A (en) * 1986-12-03 1991-01-22 University Patents, Inc. RNA ribozyme polymerases, dephosphorylases, restriction endoribonucleases and methods
US4795699A (en) * 1987-01-14 1989-01-03 President And Fellows Of Harvard College T7 DNA polymerase
EP0300422B1 (de) * 1987-07-21 1992-12-02 The Du Pont Merck Pharmaceutical Company Verfahren für die Herstellung von in tierischen Zellen stabilen und lebensfähigen rekombinanten viralen Vektoren
US5204244A (en) * 1987-10-27 1993-04-20 Oncogen Production of chimeric antibodies by homologous recombination
NZ227934A (en) 1988-02-12 1991-06-25 Commw Scient Ind Res Org Fowlpox virus vectors
US5378618A (en) * 1988-04-15 1995-01-03 E. I. Du Pont De Nemours And Company Vitro headful packaging system for cloning DNA fragments as large as 95kb
US5334375A (en) * 1988-12-29 1994-08-02 Colgate Palmolive Company Antibacterial antiplaque oral composition
DE3901681A1 (de) * 1989-01-21 1990-07-26 Behringwerke Ag Signalpeptid fuer die sekretion von peptiden in escherichia coli, verfahren zu seiner gewinnung und seine verwendung
CA2012983A1 (en) * 1989-03-27 1990-09-27 Sydney Brenner Process for nucleic acid detection by binary amplification
US5102797A (en) * 1989-05-26 1992-04-07 Dna Plant Technology Corporation Introduction of heterologous genes into bacteria using transposon flanked expression cassette and a binary vector system
US6040138A (en) * 1995-09-15 2000-03-21 Affymetrix, Inc. Expression monitoring by hybridization to high density oligonucleotide arrays
US5744101A (en) * 1989-06-07 1998-04-28 Affymax Technologies N.V. Photolabile nucleoside protecting groups
US6309822B1 (en) * 1989-06-07 2001-10-30 Affymetrix, Inc. Method for comparing copy number of nucleic acid sequences
US6346413B1 (en) * 1989-06-07 2002-02-12 Affymetrix, Inc. Polymer arrays
EP0489002B1 (de) 1989-08-22 1994-12-28 E.I. Du Pont De Nemours And Company In vitro-verpackungsvorrichtung um dna-fragmente von 95-kb zu klonen
NO904633L (no) 1989-11-09 1991-05-10 Molecular Diagnostics Inc Amplifikasjon av nukleinsyrer ved transkriberbar haarnaalsprobe.
CA2071943C (en) 1989-12-22 2007-04-24 Joan Tellefsen Odell Site-specific recombination of dna in plant cells
US5658772A (en) 1989-12-22 1997-08-19 E. I. Du Pont De Nemours And Company Site-specific recombination of DNA in plant cells
DE69033732T2 (de) * 1990-01-08 2001-10-11 Stratagene, La Jolla Neue Wirtsorganismen zur Klonierung
US5283173A (en) * 1990-01-24 1994-02-01 The Research Foundation Of State University Of New York System to detect protein-protein interactions
US5192675A (en) * 1990-03-20 1993-03-09 Life Technologies, Inc. Cloned KpnI restriction-modification system
US5082784A (en) * 1990-03-20 1992-01-21 Life Technologies, Inc. Cloned kpni restriction-modification system
EP0528881A4 (en) * 1990-04-24 1993-05-26 Stratagene Methods for phenotype creation from multiple gene populations
US5451513A (en) * 1990-05-01 1995-09-19 The State University of New Jersey Rutgers Method for stably transforming plastids of multicellular plants
US5098839A (en) * 1990-05-10 1992-03-24 New England Biolabs, Inc. Type ii restriction endonuclease obtainable from pseudomonas alcaligenes and a process for producing the same
EP0585287B1 (de) 1990-07-10 1999-10-13 Cambridge Antibody Technology Limited Verfahren zur herstellung von spezifischen bindungspaargliedern
US5179015A (en) * 1990-07-23 1993-01-12 New England Biolabs, Inc. Heterospecific modification as a means to clone restriction genes
EP1790723A3 (de) 1990-09-27 2008-10-22 Invitrogen Corporation Direktes Klonen von PCR-amplifizierten Nukleinsäuren
US5227288A (en) 1990-10-01 1993-07-13 Blattner Frederick R DNA sequencing vector with reversible insert
FR2670502B1 (fr) 1990-12-13 1994-09-16 Eurolysine Cassette d'integration "multisite" dans le genome d'une levure, levure transformee et procede de preparation d'un produit d'interet par une levure ainsi transformee.
US5286632A (en) * 1991-01-09 1994-02-15 Jones Douglas H Method for in vivo recombination and mutagenesis
US5348788A (en) * 1991-01-30 1994-09-20 Interpore Orthopaedics, Inc. Mesh sheet with microscopic projections and holes
WO1992015694A1 (en) 1991-03-08 1992-09-17 The Salk Institute For Biological Studies Flp-mediated gene modification in mammalian cells, and compositions and cells useful therefor
US5962255A (en) * 1992-03-24 1999-10-05 Cambridge Antibody Technology Limited Methods for producing recombinant vectors
US5871907A (en) 1991-05-15 1999-02-16 Medical Research Council Methods for producing members of specific binding pairs
US5858657A (en) 1992-05-15 1999-01-12 Medical Research Council Methods for producing members of specific binding pairs
WO1992022650A1 (en) 1991-06-17 1992-12-23 Life Technologies, Inc. Recombination-facilitated multiplex analysis of dna fragments
EP0625052A4 (de) 1991-10-21 1995-07-19 Medimmune Inc Sekretionssignal von lipoproteinen kodierende dna enthaltener bakterieller expressionsvektor.
GB9123929D0 (en) 1991-11-11 1992-01-02 Wellcome Found Novel expression system
ZA929351B (en) * 1991-12-11 1993-06-04 Igen Inc Electrochemiluminescent label for DNA assays.
EP0626999A4 (en) 1992-01-24 1997-04-09 Life Technologies Inc Modulation of enzyme activities in the -i(in vivo) cloning of dna.
ES2157217T3 (es) 1992-02-26 2001-08-16 Zeneca Mogen B V Cepas de agrobacterium capaces de recombinacion especifica para el sitio.
JPH05236997A (ja) 1992-02-28 1993-09-17 Hitachi Ltd ポリヌクレオチド捕捉用チップ
JP3507073B2 (ja) * 1992-03-24 2004-03-15 ケンブリッジ アンティボディー テクノロジー リミティド 特異的結合対の成員の製造方法
US5733743A (en) 1992-03-24 1998-03-31 Cambridge Antibody Technology Limited Methods for producing members of specific binding pairs
US5286393A (en) * 1992-04-15 1994-02-15 Jet-Lube, Inc. Coating and bonding composition
US5285173A (en) 1992-07-13 1994-02-08 Analog Devices, Inc. Signal-controlled ring oscillator with delay cells having constant gain with change in frequency
BE1006085A3 (fr) * 1992-07-31 1994-05-10 Univ Bruxelles Vecteur de clonage.
US7176029B2 (en) * 1992-07-31 2007-02-13 Universite Libre De Bruxelles Cloning and/or sequencing vector
US5733733A (en) 1992-08-04 1998-03-31 Replicon, Inc. Methods for the isothermal amplification of nucleic acid molecules
WO1994003624A1 (en) 1992-08-04 1994-02-17 Auerbach Jeffrey I Methods for the isothermal amplification of nucleic acid molecules
US5614389A (en) 1992-08-04 1997-03-25 Replicon, Inc. Methods for the isothermal amplification of nucleic acid molecules
US5348886A (en) * 1992-09-04 1994-09-20 Monsanto Company Method of producing recombinant eukaryotic viruses in bacteria
US6225121B1 (en) * 1992-09-14 2001-05-01 Institute Of Molecular Biology And Biotechnology/Forth Eukaryotic transposable element
EP0798384A1 (de) 1992-10-02 1997-10-01 Lonza A.G. Biotechnologisches Verfahren zur Herstellung von Biotin
WO1994009127A2 (en) 1992-10-16 1994-04-28 The Government Of The United States Of America As Represented By The Secretary, Department Of Healthand Human Services Supercoiled minicircle dna as as a unitary promoter vector
JP3720353B2 (ja) 1992-12-04 2005-11-24 メディカル リサーチ カウンシル 多価および多重特異性の結合タンパク質、それらの製造および使用
US5334575A (en) * 1992-12-17 1994-08-02 Eastman Kodak Company Dye-containing beads for laser-induced thermal dye transfer
US5527695A (en) * 1993-01-29 1996-06-18 Purdue Research Foundation Controlled modification of eukaryotic genomes
GB9302798D0 (en) * 1993-02-12 1993-03-31 Medical Res Council Improvements in or relating to cloning dna
AU6359894A (en) 1993-03-05 1994-09-26 Crop Genetics International Corporation Agricultural-chemical-producing endosymbiotic microorganisms and method of preparing and using same
FR2705686B1 (fr) * 1993-05-28 1995-08-18 Transgene Sa Nouveaux adénovirus défectifs et lignées de complémentation correspondantes.
US5834256A (en) * 1993-06-11 1998-11-10 Cell Genesys, Inc. Method for production of high titer virus and high efficiency retroviral mediated transduction of mammalian cells
ATE253635T1 (de) 1993-06-15 2003-11-15 Du Pont Rekombinante spinnerseide analoge
US6140087A (en) * 1993-06-24 2000-10-31 Advec, Inc. Adenovirus vectors for gene therapy
US6120764A (en) * 1993-06-24 2000-09-19 Advec, Inc. Adenoviruses for control of gene expression
US5919676A (en) 1993-06-24 1999-07-06 Advec, Inc. Adenoviral vector system comprising Cre-loxP recombination
EP0632054A1 (de) * 1993-06-28 1995-01-04 European Molecular Biology Laboratory Regulation stellenspezifischer Rekombination mittels stellungsspezifischer Rekombinase/Kernrezeptor-Fusionsproteine
US5843744A (en) 1993-07-08 1998-12-01 Ecogen Inc. Bacillus thuringiensis Tn5401 proteins
US5441884A (en) 1993-07-08 1995-08-15 Ecogen Inc. Bacillus thuringiensis transposon TN5401
US5470727A (en) 1993-12-21 1995-11-28 Celtrix Pharmaceuticals, Inc. Chromosomal expression of non-bacterial genes in bacterial cells
US5861273A (en) 1993-12-21 1999-01-19 Celtrix Phamraceuticals, Inc. Chromosomal expression of heterologous genes in bacterial cells
FR2715940B1 (fr) 1994-02-10 1996-04-26 Pasteur Merieux Serums Vacc Procédé de préparation d'une banque multicombinatoire de vecteurs d'expression de gènes d'anticorps, banque et systèmes d'expression d'anticorps "coliclonaux" obtenus.
US5605793A (en) * 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US5837458A (en) * 1994-02-17 1998-11-17 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US5492841A (en) * 1994-02-18 1996-02-20 E. I. Du Pont De Nemours And Company Quaternary ammonium immunogenic conjugates and immunoassay reagents
US5677170A (en) * 1994-03-02 1997-10-14 The Johns Hopkins University In vitro transposition of artificial transposons
WO1995032225A1 (en) 1994-05-23 1995-11-30 The Salk Institute For Biological Studies Method for site-specific integration of nucleic acids and related products
EG23907A (en) 1994-08-01 2007-12-30 Delta & Pine Land Co Control of plant gene expression
US5723765A (en) 1994-08-01 1998-03-03 Delta And Pine Land Co. Control of plant gene expression
US5589618A (en) * 1994-09-01 1996-12-31 University Of Florida Materials and methods for increasing corn seed weight
US5601980A (en) * 1994-09-23 1997-02-11 Hewlett-Packard Company Manufacturing method and apparatus for biological probe arrays using vision-assisted micropipetting
US5605802A (en) * 1994-09-30 1997-02-25 The Salk Institute For Biological Studies Method for identifying agents which block infection of cells by HIV
US6015668A (en) * 1994-09-30 2000-01-18 Life Technologies, Inc. Cloned DNA polymerases from thermotoga and mutants thereof
US5614365A (en) * 1994-10-17 1997-03-25 President & Fellow Of Harvard College DNA polymerase having modified nucleotide binding site for DNA sequencing
FR2727689A1 (fr) * 1994-12-01 1996-06-07 Transgene Sa Nouveau procede de preparation d'un vecteur viral
US5766891A (en) 1994-12-19 1998-06-16 Sloan-Kettering Institute For Cancer Research Method for molecular cloning and polynucleotide synthesis using vaccinia DNA topoisomerase
US5981177A (en) * 1995-01-25 1999-11-09 Demirjian; David C. Protein fusion method and constructs
FR2731014B1 (fr) 1995-02-23 1997-03-28 Rhone Poulenc Rorer Sa Molecules d'adn, preparation et utilisation en therapie genique
US5599695A (en) * 1995-02-27 1997-02-04 Affymetrix, Inc. Printing molecular library arrays using deprotection agents solely in the vapor phase
US5814300A (en) * 1995-03-03 1998-09-29 Cephalon, Inc. Gene-targeted non-human mammals deficient in the SOD-1 gene
US6130364A (en) 1995-03-29 2000-10-10 Abgenix, Inc. Production of antibodies using Cre-mediated site-specific recombination
US5695971A (en) * 1995-04-07 1997-12-09 Amresco Phage-cosmid hybrid vector, open cos DNA fragments, their method of use, and process of production
US6143557A (en) * 1995-06-07 2000-11-07 Life Technologies, Inc. Recombination cloning using engineered recombination sites
US6720140B1 (en) * 1995-06-07 2004-04-13 Invitrogen Corporation Recombinational cloning using engineered recombination sites
CA2226463A1 (en) 1995-06-07 1996-12-19 Life Technologies Corporation Recombinational cloning using engineered recombination sites
US6964861B1 (en) * 1998-11-13 2005-11-15 Invitrogen Corporation Enhanced in vitro recombinational cloning of using ribosomal proteins
US5877021A (en) * 1995-07-07 1999-03-02 Ribozyme Pharmaceuticals, Inc. B7-1 targeted ribozymes
US6455254B1 (en) 1995-07-18 2002-09-24 Diversa Corporation Sequence based screening
FR2737222B1 (fr) * 1995-07-24 1997-10-17 Transgene Sa Nouveaux vecteurs viraux et lignee pour la therapie genique
WO1997006265A2 (en) 1995-08-07 1997-02-20 The Perkin-Elmer Corporation Recombinant clone selection system
US5801030A (en) * 1995-09-01 1998-09-01 Genvec, Inc. Methods and vectors for site-specific recombination
AUPN523995A0 (en) 1995-09-05 1995-09-28 Crc For Biopharmaceutical Research Pty Ltd Method for producing phage display vectors
DE69628026T2 (de) * 1995-09-07 2004-04-08 Novozymes A/S Nachweis der aktivität von enzymen für detergenzien durch phagen.
US20030027296A1 (en) * 1995-09-08 2003-02-06 Deb K. Chatterjee Cloned dna polymerases from thermotoga maritima and mutants thereof
US6013516A (en) * 1995-10-06 2000-01-11 The Salk Institute For Biological Studies Vector and method of use for nucleic acid delivery to non-dividing cells
WO1997013401A1 (en) * 1995-10-13 1997-04-17 Purdue Research Foundation Method for the production of hybrid plants
CA2231582A1 (en) * 1995-10-17 1997-04-24 Genencor International, Inc. Enzymatic array and process of making same
US5695917A (en) * 1995-11-22 1997-12-09 Eastman Kodak Company Combination of yellow filter dye and 4-equivalent pyrazolone magenta coupler
US6238884B1 (en) 1995-12-07 2001-05-29 Diversa Corporation End selection in directed evolution
US6358709B1 (en) 1995-12-07 2002-03-19 Diversa Corporation End selection in directed evolution
US6537776B1 (en) 1999-06-14 2003-03-25 Diversa Corporation Synthetic ligation reassembly in directed evolution
US6479258B1 (en) 1995-12-07 2002-11-12 Diversa Corporation Non-stochastic generation of genetic vaccines
US5965408A (en) 1996-07-09 1999-10-12 Diversa Corporation Method of DNA reassembly by interrupting synthesis
US6713279B1 (en) * 1995-12-07 2004-03-30 Diversa Corporation Non-stochastic generation of genetic vaccines and enzymes
US6740506B2 (en) * 1995-12-07 2004-05-25 Diversa Corporation End selection in directed evolution
US6489145B1 (en) 1996-07-09 2002-12-03 Diversa Corporation Method of DNA shuffling
US6171820B1 (en) 1995-12-07 2001-01-09 Diversa Corporation Saturation mutagenesis in directed evolution
US6361974B1 (en) 1995-12-07 2002-03-26 Diversa Corporation Exonuclease-mediated nucleic acid reassembly in directed evolution
US6352842B1 (en) 1995-12-07 2002-03-05 Diversa Corporation Exonucease-mediated gene assembly in directed evolution
CA2239366A1 (en) 1996-01-05 1997-07-17 Genetic Therapy, Inc. Recombinase-mediated generation of adenoviral vectors
AU2319497A (en) * 1996-03-07 1997-09-22 Regents Of The University Of California, The Helper-free, totally defective adenovirus for gene therapy
US6013440A (en) * 1996-03-11 2000-01-11 Affymetrix, Inc. Nucleic acid affinity columns
JP3346976B2 (ja) * 1996-03-18 2002-11-18 理化学研究所 完全長cDNAライブラリーの作成方法
US5721435A (en) * 1996-04-09 1998-02-24 Hewlett Packard Company Methods and apparatus for measuring optical properties of biological and chemical substances
US6556712B1 (en) 1996-05-23 2003-04-29 Apple Computer, Inc. Methods and apparatus for handwriting recognition
US5928914A (en) * 1996-06-14 1999-07-27 Albert Einstein College Of Medicine Of Yeshiva University, A Division Of Yeshiva University Methods and compositions for transforming cells
US5710248A (en) 1996-07-29 1998-01-20 University Of Iowa Research Foundation Peptide tag for immunodetection and immunopurification
IL128736A0 (en) 1996-09-06 2000-01-31 Univ Pennsylvania Methods using cre-lox for production of recombinant adeno-associated viruses
US6025192A (en) * 1996-09-20 2000-02-15 Cold Spring Harbor Laboratory Modified retroviral vectors
US6190907B1 (en) * 1996-09-21 2001-02-20 Viromedica Pacific Limited Retroviral vectors for gene therapy
CA2267220A1 (en) * 1996-10-11 1998-04-23 The Texas A & M University System Methods for the generation of primordial germ cells and transgenic animal species
US5962303A (en) 1996-10-15 1999-10-05 Smithkline Beecham Corporation Topoisomerase III
US6066778A (en) * 1996-11-06 2000-05-23 The Regents Of The University Of Michigan Transgenic mice expressing APC resistant factor V
BR9713368A (pt) * 1996-11-20 2001-09-18 Introgen Therapeutics Inc Processo melhorado para a produção e a purificação de vetores adenovirais
US6303301B1 (en) * 1997-01-13 2001-10-16 Affymetrix, Inc. Expression monitoring for gene function identification
US5851808A (en) 1997-02-28 1998-12-22 Baylor College Of Medicine Rapid subcloning using site-specific recombination
AU6443298A (en) 1997-02-28 1998-09-18 Nature Technology Corporation Self-assembling genes, vectors and uses thereof
US5948653A (en) * 1997-03-21 1999-09-07 Pati; Sushma Sequence alterations using homologous recombination
DE19720839B4 (de) 1997-05-17 2007-08-16 Dirk Dr. Schindelhauer Baukastenprinzip-Technologie zur Herstellung von langen, exakten DNA-Konstrukten in vitro, insbesondere für die Herstellung von Konstrukten zur Erzeugung von künstlichen Chromosomen (MAC, mammalian artificial chromosome) aus zwei stabil klonierten DNA-Komponenten und der ditelomerische Vektor-Prototyp PTAT
US5876946A (en) * 1997-06-03 1999-03-02 Pharmacopeia, Inc. High-throughput assay
DE69827745T2 (de) 1997-06-12 2005-11-10 Sloan-Kettering Institute For Cancer Research Kovalente bindung von dna zu rna strängen katalysiert durch vaccinia topoisomerase
US5856144A (en) * 1997-06-18 1999-01-05 Novagen, Inc. Direct cloning of DNA fragments
US5989872A (en) 1997-08-12 1999-11-23 Clontech Laboratories, Inc. Methods and compositions for transferring DNA sequence information among vectors
US6140086A (en) 1997-08-15 2000-10-31 Fox; Donna K. Methods and compositions for cloning nucleic acid molecules
GB9717953D0 (en) * 1997-08-22 1997-10-29 Smithkline Beecham Biolog Vaccine
US7135608B1 (en) 1997-08-28 2006-11-14 The Salk Institute For Biological Studies Site-specific recombination in eukaryotes and constructs useful therefor
US5888795A (en) * 1997-09-09 1999-03-30 Becton, Dickinson And Company Thermostable uracil DNA glycosylase and methods of use
US6897066B1 (en) * 1997-09-26 2005-05-24 Athersys, Inc. Compositions and methods for non-targeted activation of endogenous genes
CN101125873A (zh) * 1997-10-24 2008-02-20 茵维特罗根公司 利用具重组位点的核酸进行重组克隆
US7351578B2 (en) 1999-12-10 2008-04-01 Invitrogen Corp. Use of multiple recombination sites with unique specificity in recombinational cloning
DE69836092T2 (de) 1997-10-24 2007-05-10 Invitrogen Corp., Carlsbad Rekombinatorisches klonieren unter verwendung von nukleinsaüren mit rekombinationsstellen
US7393632B2 (en) * 1999-12-10 2008-07-01 Invitrogen Corp. Use of multiple recombination sites with unique specificity in recombinational cloning
JP4206154B2 (ja) 1997-11-13 2009-01-07 大日本住友製薬株式会社 変異型loxP配列とその応用
US6262341B1 (en) * 1997-11-18 2001-07-17 Pioneer Hi-Bred International, Inc. Method for the integration of foreign DNA into eukaryotic genomes
US5874259A (en) 1997-11-21 1999-02-23 Wisconsin Alumni Research Foundation Conditionally amplifiable BAC vector
CA2312474C (en) * 1997-12-05 2012-07-03 Europaisches Laboratorium Fur Molekularbiologie Novel dna cloning method
US5994136A (en) * 1997-12-12 1999-11-30 Cell Genesys, Inc. Method and means for producing high titer, safe, recombinant lentivirus vectors
WO1999032660A1 (en) * 1997-12-19 1999-07-01 Affymetrix Exploiting genomics in the search for new drugs
US6506559B1 (en) * 1997-12-23 2003-01-14 Carnegie Institute Of Washington Genetic inhibition by double-stranded RNA
US6080576A (en) * 1998-03-27 2000-06-27 Lexicon Genetics Incorporated Vectors for gene trapping and gene activation
US6436707B1 (en) * 1998-03-27 2002-08-20 Lexicon Genetics Incorporated Vectors for gene mutagenesis and gene discovery
US6024192A (en) * 1998-04-20 2000-02-15 Griffin; Maurice B. Ladder vise
WO1999055851A2 (en) 1998-04-28 1999-11-04 Novartis Ag Site-directed transformation of plants
US6197584B1 (en) * 1998-05-01 2001-03-06 Isis Pharmaceuticals, Inc. Antisense modulation of CD40 expression
US6413776B1 (en) * 1998-06-12 2002-07-02 Galapagos Geonomics N.V. High throughput screening of gene function using adenoviral libraries for functional genomics applications
US6031094A (en) * 1998-07-23 2000-02-29 The Regents Of The University Of California Beta-lactam substrates and uses thereof
GB9816761D0 (en) * 1998-07-31 1998-09-30 Phogen Limited Herpesvirus preparations and their uses
US6190908B1 (en) * 1998-08-12 2001-02-20 The Scripps Research Institute Modulation of polypeptide display on modified filamentous phage
WO2000012687A1 (en) * 1998-08-28 2000-03-09 Invitrogen Corporation System for the rapid manipulation of nucleic acid sequences
EP1131078A4 (de) 1998-11-13 2005-08-24 Invitrogen Corp Zusammensetzngen und verfahren zur rekombinatorischen klonierunf von nukleinsäure molekülen
DE60042969D1 (de) 1999-03-02 2009-10-29 Life Technologies Corp Zubereitungen und methoden zur verwendung in rekombinatorischem klonen von nukleinsäuren
JP2002537800A (ja) 1999-03-02 2002-11-12 インビトロゲン・コーポレーション 毒性遺伝子に対して耐性のある細胞およびその使用
WO2000052155A2 (en) * 1999-03-05 2000-09-08 Maxygen, Inc. Recombination of insertion modified nucleic acids
FI105584B (fi) * 1999-03-30 2000-09-15 Valmet Corp Menetelmä paperi- tai kartonkikoneen telan valmistamiseksi ja paperi- tai kartonkikoneen tela
EP1169458A2 (de) 1999-04-06 2002-01-09 Oklahoma Medical Research Foundation Verfahren zur selektion von rekombinase varianten mit veränderten spezifität
US6355412B1 (en) * 1999-07-09 2002-03-12 The European Molecular Biology Laboratory Methods and compositions for directed cloning and subcloning using homologous recombination
JP2003510019A (ja) * 1999-07-14 2003-03-18 クロンテック・ラボラトリーズ・インコーポレーテッド リコンビナーゼを利用した発現ベクターを作製する方法、およびこの方法を実施するために使用する組成物
DK1234022T3 (da) 1999-07-23 2010-07-19 Univ California DNA-rekombination i eukaryote celler med bakteriofag PHIC31-rekombinationssystemet
WO2001011058A1 (en) 1999-08-09 2001-02-15 Monsanto Technology Llc Novel cloning methods and vectors
DE19941186A1 (de) * 1999-08-30 2001-03-01 Peter Droege Sequenz-spezifische DNA-Rekombination in eukaryotischen Zellen
US20020006664A1 (en) * 1999-09-17 2002-01-17 Sabatini David M. Arrayed transfection method and uses related thereto
JP2003509060A (ja) * 1999-09-17 2003-03-11 ホワイトヘッド・インスティテュート・フォー・バイオメディカル・リサーチ 逆トランスフェクション方法
CA2388907A1 (en) 1999-10-25 2001-05-03 Life Technologies Corporation Methods of manipulating and sequencing nucleic acid molecules using transposition and recombination
JP2002076051A (ja) * 2000-09-01 2002-03-15 Nec Corp 半導体装置のボンディングパッド構造及びボンディング方法
WO2001062892A2 (en) 2000-02-25 2001-08-30 Stratagene Method for ligating nucleic acids and molecular cloning
EP1272630A2 (de) * 2000-03-16 2003-01-08 Genetica, Inc. Methoden und zusammensetzungen zur interferenz durch rna
US20020025561A1 (en) * 2000-04-17 2002-02-28 Hodgson Clague Pitman Vectors for gene-self-assembly
JP4757369B2 (ja) * 2000-05-08 2011-08-24 パナソニック株式会社 角形アルカリ蓄電池、並びにこれを用いた単位電池及び組電池
US7244560B2 (en) * 2000-05-21 2007-07-17 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
US20020068290A1 (en) * 2000-05-31 2002-06-06 Timur Yarovinsky Topoisomerase activated oligonucleotide adaptors and uses therefor
US6551828B1 (en) 2000-06-28 2003-04-22 Protemation, Inc. Compositions and methods for generating expression vectors through site-specific recombination
GB0016747D0 (en) 2000-07-08 2000-08-30 Johnson Matthey Plc Ink
CA2417130A1 (en) 2000-07-25 2002-01-31 National Research Council Of Canada Glycerol-3-phosphate/dihydroxyacetone phosphate dual substrate acyltransferases
US7198924B2 (en) 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
EP1313851B1 (de) * 2000-08-21 2006-01-04 Invitrogen Corporation Methoden und reagenzien für molekulares klonieren
JP2004537712A (ja) * 2000-10-18 2004-12-16 バーチャル・アレイズ・インコーポレーテッド 多重細胞分析システム
CA2430886A1 (en) 2000-12-08 2002-08-08 Life Technologies Corporation Compositions and methods for rapidly generating recombinant nucleic acid molecules
US20060008817A1 (en) * 2000-12-08 2006-01-12 Invitrogen Corporation Methods and compositions for generating recombinant nucleic acid molecules
US6977165B2 (en) * 2001-01-18 2005-12-20 Clontech Laboratories, Inc. Sequence specific recombinase-based methods for producing intron containing vectors and compositions for use in practicing the same
US20030176644A1 (en) * 2001-02-07 2003-09-18 Devon Byrd Compositions and methods for molecular biology
JP2004531259A (ja) 2001-04-19 2004-10-14 インヴィトロジェン コーポレーション 核酸分子の組換えクローニングのための組成物および方法
CA2448505A1 (en) 2001-05-21 2002-11-28 Invitrogen Corporation Compositions and methods for use in isolation of nucleic acid molecules
WO2003056293A2 (en) * 2001-07-10 2003-07-10 Whitehead Institute For Biomedical Research Small molecule microarrays
WO2003025161A1 (en) 2001-09-18 2003-03-27 Clontech Laboratories, Inc. Site-specific recombinase based method for producing adenoviral vectors
US20030135888A1 (en) * 2001-09-26 2003-07-17 Tong Zhu Genes that are modulated by posttranscriptional gene silencing
JP4210769B2 (ja) 2001-11-22 2009-01-21 独立行政法人産業技術総合研究所 Gatewayエントリークローンの作製方法
US7294504B1 (en) * 2001-12-27 2007-11-13 Allele Biotechnology & Pharmaceuticals, Inc. Methods and compositions for DNA mediated gene silencing
US20030220249A1 (en) * 2002-02-07 2003-11-27 Hackett Perry B. Factors for angiogenesis, vasculogenesis, cartilage formation, bone formation, and methods of use thereof
US7175806B2 (en) * 2002-03-15 2007-02-13 Deal Jeffery L C-band disinfector
EP1539998A4 (de) 2002-06-05 2007-10-31 Invitrogen Corp Verfahren und zusammensetzungen zur synthese von nukleinsäuremolekülen unter verwendung von mehrfacherkennungsstellen
US20040132133A1 (en) * 2002-07-08 2004-07-08 Invitrogen Corporation Methods and compositions for the production, identification and purification of fusion proteins
AU2003253992A1 (en) 2002-07-18 2004-02-09 Robert P. Bennett Viral vectors containing recombination sites
AU2003257109A1 (en) 2002-08-05 2004-02-23 Invitrogen Corporation Compositions and methods for molecular biology
US20040253620A1 (en) * 2003-04-30 2004-12-16 Invitrogen Corporation Use of site specific recombination to prepare molecular markers
US20050095615A1 (en) * 2003-06-26 2005-05-05 Welch Peter J. Methods and compositions for detecting promoter activity and expressing fusion proteins
EP2216415B2 (de) 2003-08-01 2017-01-04 Life Technologies Corporation Zusammensetzungen und Verfahren zur Aufreinigung kurzer RNA-Moleküle und anderer Nukleinsäuren
WO2005014796A2 (en) 2003-08-08 2005-02-17 Invitrogen Corporation Methods and compositions for seamless cloning of nucleic acid molecules
US20050176065A1 (en) * 2003-10-22 2005-08-11 Invitrogen Corporation Target sequences for synthetic molecules
JP2007512838A (ja) * 2003-12-01 2007-05-24 インヴィトロジェン コーポレーション 組換え部位を含む核酸分子およびその使用方法

Also Published As

Publication number Publication date
US20020192819A1 (en) 2002-12-19
US20090186385A1 (en) 2009-07-23
US20040253631A1 (en) 2004-12-16
AU752704B2 (en) 2002-09-26
EP1025217A1 (de) 2000-08-09
CN1280614A (zh) 2001-01-17
NZ520579A (en) 2004-08-27
HK1034287A1 (en) 2001-10-19
HK1030019A1 (en) 2001-04-20
US20090186386A1 (en) 2009-07-23
NZ533288A (en) 2005-10-28
US20050009091A1 (en) 2005-01-13
US20040171157A1 (en) 2004-09-02
EP1025217A4 (de) 2002-11-13
CA2307016A1 (en) 1999-05-06
US20040063207A1 (en) 2004-04-01
CN100342008C (zh) 2007-10-10
US20100267119A1 (en) 2010-10-21
WO1999021977A1 (en) 1999-05-06
NZ504214A (en) 2003-06-30
US20100267120A1 (en) 2010-10-21
DE69836092D1 (de) 2006-11-16
IL135776A0 (en) 2001-05-20
DE69836092T2 (de) 2007-05-10
EP1025217B1 (de) 2006-10-04
US7714116B2 (en) 2010-05-11
US7408049B2 (en) 2008-08-05
US20140004567A1 (en) 2014-01-02
US6277608B1 (en) 2001-08-21
JP4303418B2 (ja) 2009-07-29
US20030157716A1 (en) 2003-08-21
US20020172997A1 (en) 2002-11-21
WO1999021977A9 (en) 1999-07-01
AU1199599A (en) 1999-05-17
US20030175970A1 (en) 2003-09-18
JP2002500861A (ja) 2002-01-15
US20040171156A1 (en) 2004-09-02
JP2009136297A (ja) 2009-06-25

Similar Documents

Publication Publication Date Title
ATE341621T1 (de) Rekombinatorisches klonieren unter verwendung von nukleinsaüren mit rekombinationsstellen
NZ312332A (en) Recombinational cloning using engineered recombination sites
ATE443074T1 (de) Zubereitungen und methoden zur verwendung in rekombinatorischem klonen von nukleinsäuren
CY1108175T1 (el) ΠΟΛΥΠΕΠΤΙΔΙΟ DcR3, ΟΜΟΛΟΓΟ TNFR
DE60033138D1 (de) Polypeptide mit galaktoseoxidaseaktivität und dafür kodierende nukleisäuren
DK1187852T3 (da) Ekspression og eksport af interferon-alpha-proteiner som Fc-fusionsproteiner
DE60144145D1 (de) Subtilisin-variante
DK1294876T3 (da) EV-VEGF-nukleinsyrer og -polypeptider samt fremgangsmåder til deres benyttelse
ATE184052T1 (de) Gdf-1 und uog1 proteine
BR9814404A (pt) " clonagem de expressão em fungos filamentosos "
NO960767L (no) Protocadherinproteiner og deres anvendelse
ATE284954T1 (de) Hyaluronan synthase gen und seine verwendung
DE69930955D1 (de) Polypeptide mit lysophospholipaseaktivität und nukleinesäuren die sie kodieren
ATE325533T1 (de) Verfahren zum verbesserten virusvermittelten dns- transfer unter verwendung von molekülen mit virus-und zellbindenden domänen
ATE450603T1 (de) Polypeptide mit verzweigungsenzym-aktivität und für diese kodierende nukleinsäuren
DE69837475D1 (de) Polypeptide mit aminopeptidaseaktivität und dafür kodierende nukleinsäuren
DK1025245T3 (da) Hurtig etablering af stabile pattedyrscelelinjer, der producerer höje koncentrationer af rekombinante proteiner
DK1064018T3 (da) Anvendelsen af isolerede domæner af Type IV kollagen til at modificere celle- og vævsinteraktioner
DE69840326D1 (de) Steigerung der immunantwort unter verwendung von zielgerichteten molekülen
ATE309370T1 (de) Retinoid-metabolisierendes protein
ATE346919T1 (de) Nebenhoden-spezifisches rezeptorprotein und dessen verwendung
KR930008148A (ko) 변이 인간 프로우로키나아제
ATE131175T1 (de) Protein mit fibroblasten-wachstumsfaktor- rezeptoraktivität.
DE60026049D1 (de) POLYPEPTIDE MIT GLUCANOTRANSFERASE AKTIVITÄT UND DAFüR KODIERENDE NUKLEINSÄUREN
DK0742829T3 (da) Ekspression af heterologe proteiner i svækkede bakterier under anvendelse af HTRA-promotorerne

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties