ATE457065T1 - Verfahren zum analytennachweis unter verwendung von proximitätssonden - Google Patents

Verfahren zum analytennachweis unter verwendung von proximitätssonden

Info

Publication number
ATE457065T1
ATE457065T1 AT07732076T AT07732076T ATE457065T1 AT E457065 T1 ATE457065 T1 AT E457065T1 AT 07732076 T AT07732076 T AT 07732076T AT 07732076 T AT07732076 T AT 07732076T AT E457065 T1 ATE457065 T1 AT E457065T1
Authority
AT
Austria
Prior art keywords
proximity
analyte
nucleic acid
probes
proximity probe
Prior art date
Application number
AT07732076T
Other languages
English (en)
Inventor
Edith Schallmeiner
Original Assignee
Olink Ab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Olink Ab filed Critical Olink Ab
Application granted granted Critical
Publication of ATE457065T1 publication Critical patent/ATE457065T1/de

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • C12Q1/682Signal amplification
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Hematology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Cell Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Prostheses (AREA)
  • Geophysics And Detection Of Objects (AREA)
AT07732076T 2006-03-20 2007-03-20 Verfahren zum analytennachweis unter verwendung von proximitätssonden ATE457065T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB0605584.2A GB0605584D0 (en) 2006-03-20 2006-03-20 Method for analyte detection using proximity probes
PCT/GB2007/000984 WO2007107743A1 (en) 2006-03-20 2007-03-20 Method for analyte detection using proximity probes

Publications (1)

Publication Number Publication Date
ATE457065T1 true ATE457065T1 (de) 2010-02-15

Family

ID=36293119

Family Applications (1)

Application Number Title Priority Date Filing Date
AT07732076T ATE457065T1 (de) 2006-03-20 2007-03-20 Verfahren zum analytennachweis unter verwendung von proximitätssonden

Country Status (7)

Country Link
US (1) US8268554B2 (de)
EP (1) EP1996941B1 (de)
JP (1) JP5144639B2 (de)
AT (1) ATE457065T1 (de)
DE (1) DE602007004666D1 (de)
GB (1) GB0605584D0 (de)
WO (1) WO2007107743A1 (de)

Families Citing this family (165)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007117444A2 (en) * 2006-03-31 2007-10-18 Yinghe Hu Protein detection by aptamers
US20070281367A1 (en) * 2006-05-03 2007-12-06 Applera Corporation Methods, Compositions, and Kits for Quantitating Antibodies
US8034569B2 (en) * 2008-06-06 2011-10-11 Biotex, Inc. Methods for molecular detection
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
CN102947703A (zh) 2010-06-17 2013-02-27 皇家飞利浦电子股份有限公司 多表位测定
WO2012006556A2 (en) * 2010-07-08 2012-01-12 Life Technologies Corporation Detection of analytes
GB201011971D0 (en) * 2010-07-15 2010-09-01 Olink Ab Methods and product
US10669569B2 (en) 2010-10-15 2020-06-02 Navinci Diagnostics Ab Dynamic range methods
WO2012057689A1 (en) * 2010-10-29 2012-05-03 Ge Healthcare Bio-Sciences Ab Proximity ligation technology for western blot applications
EP2466310A1 (de) * 2010-12-17 2012-06-20 Helmholtz Zentrum München Deutsches Forschungszentrum für Gesundheit und Umwelt GmbH Mittel und Verfahren für den Nachweis von mehrstrangigen Brüchen
US9804179B2 (en) * 2011-01-08 2017-10-31 Access Medical Systems, Ltd. Systems for immunoassay tests
EP3567121B1 (de) * 2011-01-17 2023-08-30 Life Technologies Corporation Arbeitsablauf zum nachweis von liganden mit nukleinsäuren
ES2645953T3 (es) 2011-01-17 2017-12-11 Life Technologies Corporation Ligamiento enzimático de ácidos nucleicos
GB201101621D0 (en) 2011-01-31 2011-03-16 Olink Ab Method and product
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
US10597701B2 (en) 2011-05-11 2020-03-24 Navinci Diagnostics Ab Unfolding proximity probes and methods for the use thereof
GB201107863D0 (en) 2011-05-11 2011-06-22 Olink Ab Method and product
GB201108678D0 (en) 2011-05-24 2011-07-06 Olink Ab Multiplexed proximity ligation assay
EP3604555B1 (de) 2011-10-14 2024-12-25 President and Fellows of Harvard College Sequenzierung durch strukturanordnung
EP4249605B1 (de) 2011-12-22 2024-08-28 President And Fellows Of Harvard College Verfahren zum nachweis von analyten
ES2991004T3 (es) 2011-12-22 2024-12-02 Harvard College Métodos para la detección de analitos
US9029086B2 (en) 2012-01-26 2015-05-12 Masood Kamali Moghaddam Detection of single and multimodal analytes
GB201201547D0 (en) * 2012-01-30 2012-03-14 Olink Ab Method and product
US9914967B2 (en) 2012-06-05 2018-03-13 President And Fellows Of Harvard College Spatial sequencing of nucleic acids using DNA origami probes
EP4592400A3 (de) 2012-10-17 2025-10-29 10x Genomics Sweden AB Verfahren und produkt zur optimierung einer lokalisierten oder räumlichen detektion einer genexpression in einer gewebeprobe
EP2971184B1 (de) 2013-03-12 2019-04-17 President and Fellows of Harvard College Verfahren zur erzeugung einer dreidimensionalen nukleinsäure mit matrix
US10114015B2 (en) 2013-03-13 2018-10-30 Meso Scale Technologies, Llc. Assay methods
CA2904181A1 (en) 2013-03-13 2014-10-09 Anahit Aghvanyan Sandwich immunoassay comprising anchoring reagent
US9593364B2 (en) 2013-05-21 2017-03-14 Src, Inc. Detecting a target molecule in a sample using a dual-antibody quantitative fluorescence-based detection method
SG10201913068PA (en) 2013-06-04 2020-02-27 Harvard College Rna-guided transcriptional regulation
CA2916662C (en) 2013-06-25 2022-03-08 Prognosys Biosciences, Inc. Methods and systems for determining spatial patterns of biological targets in a sample
US20160146804A1 (en) * 2013-06-27 2016-05-26 The Board Of Trustees Of The Leland Stanford Junior University Dna-conjugated antibodies for improved antibody affinity and reduced antibody cross reactivity
US9957554B1 (en) 2013-12-19 2018-05-01 National Technology & Engineering Solutions Of Sandia, Llc Microfluidic platform for multiplexed detection in single cells and methods thereof
EP3143401A4 (de) 2014-05-15 2017-10-11 Meso Scale Technologies, LLC Verbesserte testverfahren
DE102014209393B4 (de) * 2014-05-19 2016-02-04 Trumpf Werkzeugmaschinen Gmbh + Co. Kg Verfahren zum maschinellen Bearbeiten eines plattenartigen Werkstücks, insbesondere einer Blechtafel
WO2016007839A1 (en) 2014-07-11 2016-01-14 President And Fellows Of Harvard College Methods for high-throughput labelling and detection of biological features in situ using microscopy
KR20170064540A (ko) * 2014-09-26 2017-06-09 투 포어 가이즈, 인코포레이티드 합성 프로브의 나노포어 감지에 의한 표적 서열 검출
EP3901282B1 (de) 2015-04-10 2023-06-28 Spatial Transcriptomics AB Räumlich getrennte multiplex-nukleinsäureanalyse von biologischen proben
MX2018003532A (es) 2015-09-24 2019-04-25 Abvitro Llc Conjugados de afinidad-oligonucleotido y usos de los mismos.
GB201518655D0 (en) 2015-10-21 2015-12-02 Olink Ab Method for generating proximity probes
AU2016349288A1 (en) 2015-11-03 2018-05-31 President And Fellows Of Harvard College Method and apparatus for volumetric imaging of a three-dimensional nucleic acid containing matrix
US11486873B2 (en) 2016-03-31 2022-11-01 Ontera Inc. Multipore determination of fractional abundance of polynucleotide sequences in a sample
EP4613756A3 (de) 2016-04-25 2025-11-12 President And Fellows Of Harvard College Hybridisierungskettenreaktionsverfahren für den in-situ-molekularnachweis
US20170342463A1 (en) * 2016-05-25 2017-11-30 Bio-Rad Laboratories, Inc. Digital proximity assay
US11352667B2 (en) 2016-06-21 2022-06-07 10X Genomics, Inc. Nucleic acid sequencing
CN118853848A (zh) 2016-08-31 2024-10-29 哈佛学院董事及会员团体 将生物分子的检测组合到使用荧光原位测序的单个试验的方法
CN118389650A (zh) 2016-08-31 2024-07-26 哈佛学院董事及会员团体 生成用于通过荧光原位测序检测的核酸序列文库的方法
JP6929354B2 (ja) 2016-09-24 2021-09-01 アブビトロ, エルエルシー 親和性−オリゴヌクレオチドコンジュゲートおよびその使用
EP3548652B1 (de) 2016-12-01 2024-04-03 Nautilus Subsidiary, Inc. Verfahren zur untersuchung von proteinen
GB201700567D0 (en) 2017-01-12 2017-03-01 Genagon Therapeutics Ab Therapeutic agents
JP7248368B2 (ja) * 2017-03-01 2023-03-29 ザ ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティー 高度に特異的な環状近接ライゲーションアッセイ
GB201703625D0 (en) * 2017-03-07 2017-04-19 Quantumdx Group Ltd Aptamer based methods for protein detection
CN120210336A (zh) 2017-10-06 2025-06-27 10X基因组学有限公司 Rna模板化连接
KR20250152680A (ko) 2017-12-29 2025-10-23 노틸러스 서브시디어리, 인크. 단백질 식별을 위한 디코딩 접근법
SG11202009802WA (en) 2018-04-03 2020-11-27 The Royal Institution For The Advancement Of Learning/Mcgill Univ Colocalization-by-linkage sandwich assays
WO2019238944A1 (en) 2018-06-15 2019-12-19 Olink Proteomics Ab Biomarker panel for ovarian cancer
CN112770776B (zh) 2018-07-30 2025-08-19 瑞德库尔有限责任公司 用于样品处理或分析的方法和系统
US11519033B2 (en) 2018-08-28 2022-12-06 10X Genomics, Inc. Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample
US12528082B2 (en) 2018-10-01 2026-01-20 University Of Washington Lateral flow-based systems and methods
WO2020076976A1 (en) 2018-10-10 2020-04-16 Readcoor, Inc. Three-dimensional spatial molecular indexing
WO2020123316A2 (en) 2018-12-10 2020-06-18 10X Genomics, Inc. Methods for determining a location of a biological analyte in a biological sample
US12529094B2 (en) 2018-12-10 2026-01-20 10X Genomics, Inc. Imaging system hardware
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
CN113748217B (zh) 2019-03-01 2023-08-25 仁慈生物分析公司 用于靶实体检测的系统、组合物和方法
CN120098057A (zh) 2019-03-01 2025-06-06 中尺度技术有限责任公司 用于免疫方法的被电化学发光标记的探针、使用这类探针的方法和包含这类探针的试剂盒
WO2020243579A1 (en) 2019-05-30 2020-12-03 10X Genomics, Inc. Methods of detecting spatial heterogeneity of a biological sample
CN119220641A (zh) 2019-05-31 2024-12-31 10X基因组学有限公司 检测目标核酸分子的方法
CN115380218A (zh) 2019-10-02 2022-11-22 诺米克生物公司 用于多重化的连接-共定位夹心测定
WO2021092433A2 (en) 2019-11-08 2021-05-14 10X Genomics, Inc. Enhancing specificity of analyte binding
EP4069731A4 (de) 2019-12-03 2024-05-29 Alamar Biosciences, Inc. Nukleinsäure-gekoppelter immun-sandwich-assay (nulisa)
GB201919032D0 (en) 2019-12-20 2020-02-05 Cartana Ab Method of detecting an analyte
ES2982420T3 (es) 2019-12-23 2024-10-16 10X Genomics Inc Métodos para el análisis espacial mediante el uso de la ligazón con plantilla de ARN
EP4081656A1 (de) 2019-12-23 2022-11-02 10X Genomics, Inc. Zusammensetzungen und verfahren zur verwendung von fixierten biologischen proben bei partitionsbasierten tests
CN115135984B (zh) 2019-12-23 2025-12-23 10X基因组学有限公司 可逆固定试剂及其使用方法
US12365942B2 (en) 2020-01-13 2025-07-22 10X Genomics, Inc. Methods of decreasing background on a spatial array
US12405264B2 (en) 2020-01-17 2025-09-02 10X Genomics, Inc. Electrophoretic system and method for analyte capture
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US20210230681A1 (en) 2020-01-24 2021-07-29 10X Genomics, Inc. Methods for spatial analysis using proximity ligation
EP4097251A1 (de) 2020-01-29 2022-12-07 10X Genomics, Inc. Zusammensetzungen und verfahren zur analytdetektion
US12076701B2 (en) 2020-01-31 2024-09-03 10X Genomics, Inc. Capturing oligonucleotides in spatial transcriptomics
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
US12110548B2 (en) 2020-02-03 2024-10-08 10X Genomics, Inc. Bi-directional in situ analysis
US12110541B2 (en) 2020-02-03 2024-10-08 10X Genomics, Inc. Methods for preparing high-resolution spatial arrays
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
WO2021158925A1 (en) 2020-02-07 2021-08-12 10X Genomics, Inc. Quantitative and automated permeabilization performance evaluation for spatial transcriptomics
US12281357B1 (en) 2020-02-14 2025-04-22 10X Genomics, Inc. In situ spatial barcoding
CN115485391A (zh) 2020-02-17 2022-12-16 10X基因组学有限公司 染色质相互作用的原位分析
EP4484571A3 (de) 2020-02-21 2025-03-19 10X Genomics, Inc. Verfahren und zusammensetzungen für integrierten räumlichen in-situ-assay
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
US12188085B2 (en) 2020-03-05 2025-01-07 10X Genomics, Inc. Three-dimensional spatial transcriptomics with sequencing readout
GB202004472D0 (en) 2020-03-27 2020-05-13 Olink Proteomics Ab Method for detecting analytes of varying abundance
WO2021191442A1 (en) 2020-03-27 2021-09-30 Olink Proteomics Ab Method for detecting analytes of varying abundance
GB202004469D0 (en) 2020-03-27 2020-05-13 Olink Proteomics Ab Controls for proximity detection assays
EP4139485B1 (de) 2020-04-22 2023-09-06 10X Genomics, Inc. Verfahren zur räumlichen analyse unter verwendung von gezieltem rna-abbau
EP4153776B1 (de) 2020-05-22 2025-03-05 10X Genomics, Inc. Räumliche analyse zur erkennung von sequenzvarianten
EP4414459B1 (de) 2020-05-22 2025-09-03 10X Genomics, Inc. Simultane räumlich-zeitliche messung der genexpression und der zellaktivität
US12031177B1 (en) 2020-06-04 2024-07-09 10X Genomics, Inc. Methods of enhancing spatial resolution of transcripts
EP4421186B1 (de) 2020-06-08 2025-08-13 10X Genomics, Inc. Verfahren zur bestimmung eines chirurgischen randes und verfahren zur verwendung davon
WO2021252800A1 (en) 2020-06-11 2021-12-16 Nautilus Biotechnology, Inc. Methods and systems for computational decoding of biological, chemical, and physical entities
US12209273B2 (en) 2020-06-12 2025-01-28 10X Genomics, Inc. Nucleic acid assays using click chemistry bioconjugation
ES2994976T3 (en) 2020-06-25 2025-02-05 10X Genomics Inc Spatial analysis of dna methylation
US11981960B1 (en) 2020-07-06 2024-05-14 10X Genomics, Inc. Spatial analysis utilizing degradable hydrogels
US12209280B1 (en) 2020-07-06 2025-01-28 10X Genomics, Inc. Methods of identifying abundance and location of an analyte in a biological sample using second strand synthesis
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
US12297499B2 (en) 2020-08-17 2025-05-13 10X Genomics, Inc. Multicomponent nucleic acid probes for sample analysis
US20220049303A1 (en) 2020-08-17 2022-02-17 Readcoor, Llc Methods and systems for spatial mapping of genetic variants
US11981958B1 (en) 2020-08-20 2024-05-14 10X Genomics, Inc. Methods for spatial analysis using DNA capture
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
US12071667B2 (en) 2020-11-04 2024-08-27 10X Genomics, Inc. Sequence analysis using meta-stable nucleic acid molecules
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
GB202018503D0 (en) 2020-11-25 2021-01-06 Olink Proteomics Ab Analyte detection method employing concatamers
US12509717B2 (en) 2020-12-11 2025-12-30 10X Genomics, Inc. Methods and compositions for multimodal in situ analysis
EP4729631A2 (de) 2020-12-21 2026-04-22 10X Genomics, Inc. Verfahren, zusammensetzungen und systeme zur erfassung von sonden und/oder barcodes
US12060603B2 (en) 2021-01-19 2024-08-13 10X Genomics, Inc. Methods for internally controlled in situ assays using padlock probes
EP4281774B1 (de) 2021-01-20 2025-11-26 Nautilus Subsidiary, Inc. Quantifizierung von biomolekülen
WO2022159663A1 (en) 2021-01-21 2022-07-28 Nautilus Biotechnology, Inc. Systems and methods for biomolecule preparation
US12275984B2 (en) 2021-03-02 2025-04-15 10X Genomics, Inc. Sequential hybridization and quenching
IL305336B2 (en) 2021-03-11 2025-09-01 Nautilus Subsidiary Inc Systems and methods for preserving biomolecules
EP4305196B1 (de) 2021-04-14 2025-04-02 10X Genomics, Inc. Verfahren zur messung der fehllokalisierung eines analyten
EP4320271B1 (de) 2021-05-06 2025-03-19 10X Genomics, Inc. Verfahren zur erhöhung der auflösung von räumlicher analyse
WO2022256503A1 (en) 2021-06-03 2022-12-08 10X Genomics, Inc. Methods, compositions, kits, and systems for enhancing analyte capture for spatial analysis
AU2022317053B2 (en) 2021-07-30 2025-01-02 10X Genomics, Inc. Methods and compositions for synchronizing reactions in situ
US12139751B2 (en) 2021-07-30 2024-11-12 10X Genomics, Inc. Circularizable probes for in situ analysis
US12460251B2 (en) 2021-08-03 2025-11-04 10X Genomics, Inc. Stabilization and/or compaction of nucleic acid molecules
US12391984B2 (en) 2021-08-03 2025-08-19 10X Genomics, Inc. Compositions and methods for rolling circle amplification
US12553079B2 (en) 2021-08-03 2026-02-17 10X Genomics, Inc. Nucleic acid concatemers and methods for stabilizing and/or compacting the same
US12529096B2 (en) 2021-08-03 2026-01-20 10X Genomics, Inc. Stabilization and/or compaction of nucleic acid structures
EP4446426B1 (de) 2021-08-16 2026-03-04 10X Genomics, Inc. Sonden mit einem geteilten strichcodebereich und verfahren zur verwendung
WO2023034489A1 (en) 2021-09-01 2023-03-09 10X Genomics, Inc. Methods, compositions, and kits for blocking a capture probe on a spatial array
CA3227872A1 (en) 2021-09-09 2023-03-16 James Henry JOLY Characterization and localization of protein modifications
AU2022352593B2 (en) 2021-09-22 2026-03-26 Nautilus Subsidiary, Inc. Methods and systems for determining polypeptide interactions
WO2023081728A1 (en) 2021-11-03 2023-05-11 Nautilus Biotechnology, Inc. Systems and methods for surface structuring
WO2023086880A1 (en) 2021-11-10 2023-05-19 10X Genomics, Inc. Methods, compositions, and kits for determining the location of an analyte in a biological sample
AU2022402132A1 (en) 2021-11-30 2024-05-30 Nautilus Subsidiary, Inc. Particle-based isolation of proteins and other analytes
WO2023102118A2 (en) 2021-12-01 2023-06-08 10X Genomics, Inc. Methods, compositions, and systems for improved in situ detection of analytes and spatial analysis
EP4733410A2 (de) 2022-01-21 2026-04-29 10X Genomics, Inc. Mehrere auslesesignale zur analyse einer probe
CN118974836A (zh) 2022-03-08 2024-11-15 10X基因组学有限公司 将光学拥挤最小化的原位代码设计方法
US12092578B2 (en) 2022-03-29 2024-09-17 Nautilus Subsidiary, Inc. Integrated arrays for single-analyte processes
EP4505177A1 (de) 2022-04-01 2025-02-12 10x Genomics, Inc. Zusammensetzungen und verfahren zur gezielten maskierung der autofluoreszenz
EP4515026A1 (de) 2022-04-25 2025-03-05 Nautilus Subsidiary, Inc. Systeme und verfahren zur beurteilung und verbesserung der qualität von multiplex-molekulartests
WO2023215603A1 (en) 2022-05-06 2023-11-09 10X Genomics, Inc. Methods and compositions for in situ analysis of v(d)j sequences
US20240084378A1 (en) 2022-05-11 2024-03-14 10X Genomics, Inc. Compositions and methods for in situ sequencing
EP4540607A1 (de) 2022-06-17 2025-04-23 10X Genomics, Inc. Katalytische entvernetzung von proben zur in-situ-analyse
US20250376716A1 (en) 2022-06-21 2025-12-11 Nautilus Subsidiary, Inc. Method for detecting analytes at sites of optically non-resolvable distances
WO2024040060A1 (en) 2022-08-16 2024-02-22 10X Genomics, Inc. Ap50 polymerases and uses thereof
WO2024059655A1 (en) 2022-09-15 2024-03-21 Nautilus Subsidiary, Inc. Characterizing accessibility of macromolecule structures
WO2024073599A1 (en) 2022-09-29 2024-04-04 Nautilus Subsidiary, Inc. Preparation of array surfaces for single-analyte processes
EP4482979B1 (de) 2022-11-09 2025-10-01 10X Genomics, Inc. Verfahren, zusammensetzungen und kits zur bestimmung der position mehrerer analyten in einer biologischen probe
US20240192202A1 (en) 2022-12-09 2024-06-13 Nautilus Subsidiary, Inc. Fluidic media for single-analtye arrays
US20240201182A1 (en) 2022-12-15 2024-06-20 Nautilus Subsidiary, Inc. Inhibition of photon phenomena on single molecule arrays
EP4655590A1 (de) 2023-01-27 2025-12-03 Biomerieux Modifizierter immunmolekulartest zum schnelleren nachweis von biomarkern
WO2024206122A1 (en) 2023-03-24 2024-10-03 Nautilus Subsidiary, Inc. Improved transfer of nanoparticles to array surfaces
WO2024243237A1 (en) 2023-05-24 2024-11-28 Nautilus Subsidiary, Inc. Systems and methods for machine vision
WO2025029831A1 (en) 2023-07-31 2025-02-06 10X Genomics, Inc. Methods and systems for targeted rna cleavage and target rna-primed rolling circle amplification
US20250066841A1 (en) 2023-08-24 2025-02-27 Nautilus Subsidiary, Inc. Compositions and methods for detecting binding interactions under equilibrium or non-equilibrium conditions
CN117431300A (zh) * 2023-10-24 2024-01-23 格物致和生物科技(北京)有限公司 一种检测方法和试剂盒
US20250189519A1 (en) 2023-12-11 2025-06-12 Nautilus Subsidiary, Inc. Methods and compositions of particle-based arrays
US20250205706A1 (en) 2023-12-20 2025-06-26 Nautilus Subsidiary, Inc. Fluidic devices for single-analyte assays
US20250298016A1 (en) 2024-03-22 2025-09-25 Nautilus Subsidiary, Inc. Plasmonic particle systems for single-analyte assays
US20250305034A1 (en) 2024-04-01 2025-10-02 Nautilus Subsidiary, Inc. Nanostructures for modulation of analyte conformation
WO2025252658A1 (en) 2024-06-03 2025-12-11 Olink Proteomics Ab New product and method
WO2025262178A1 (en) 2024-06-18 2025-12-26 Olink Proteomics Ab System and method for detecting analytes in a sample

Family Cites Families (53)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU530410B2 (en) * 1978-02-21 1983-07-14 Sintef Preparing aqueous emulsions
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4965188A (en) * 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4883867A (en) * 1985-11-01 1989-11-28 Becton, Dickinson And Company Detection of reticulocytes, RNA or DNA
US4800159A (en) * 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
US5266684A (en) * 1988-05-02 1993-11-30 The Reagents Of The University Of California Peptide mixtures
US5223409A (en) * 1988-09-02 1993-06-29 Protein Engineering Corp. Directed evolution of novel binding proteins
US5449603A (en) * 1989-10-24 1995-09-12 Stratagene Method for hybridizing nucleic acids using single-stranded nucleic acid binding protein
US5733523A (en) 1990-12-10 1998-03-31 Akzo Nobel N.V. Targeted delivery of a therapeutic entity using complementary oligonucleotides
CA2119126C (en) * 1991-09-16 1996-09-03 Molecular Probes, Inc. Dimers of unsymmetrical cyanine dyes
US5770358A (en) * 1991-09-18 1998-06-23 Affymax Technologies N.V. Tagged synthetic oligomer libraries
US5639603A (en) * 1991-09-18 1997-06-17 Affymax Technologies N.V. Synthesizing and screening molecular diversity
WO1993015226A1 (en) * 1992-01-28 1993-08-05 President And Fellows Of Harvard College Method for nucleic acid hybridization using single-stranded dna binding protein
US5321130A (en) * 1992-02-10 1994-06-14 Molecular Probes, Inc. Unsymmetrical cyanine dyes with a cationic side chain
US5541061A (en) * 1992-04-29 1996-07-30 Affymax Technologies N.V. Methods for screening factorial chemical libraries
AU687010B2 (en) * 1992-07-17 1998-02-19 Dana-Farber Cancer Institute Method of intracellular binding of target molecules
US5288514A (en) * 1992-09-14 1994-02-22 The Regents Of The University Of California Solid phase and combinatorial synthesis of benzodiazepine compounds on a solid support
US5565324A (en) * 1992-10-01 1996-10-15 The Trustees Of Columbia University In The City Of New York Complex combinatorial chemical libraries encoded with tags
US5721099A (en) * 1992-10-01 1998-02-24 Trustees Of Columbia University In The City Of New York Complex combinatorial chemical libraries encoded with tags
US5410030A (en) * 1993-04-05 1995-04-25 Molecular Probes, Inc. Dimers of unsymmetrical cyanine dyes containing pyridinium moieties
US5436134A (en) * 1993-04-13 1995-07-25 Molecular Probes, Inc. Cyclic-substituted unsymmetrical cyanine dyes
US5658751A (en) * 1993-04-13 1997-08-19 Molecular Probes, Inc. Substituted unsymmetrical cyanine dyes with selected permeability
US5440016A (en) * 1993-06-18 1995-08-08 Torrey Pines Institute For Molecular Studies Peptides of the formula (KFmoc) ZZZ and their uses
US5851829A (en) * 1993-07-16 1998-12-22 Dana-Farber Cancer Institute Method of intracellular binding of target molecules
US5593853A (en) * 1994-02-09 1997-01-14 Martek Corporation Generation and screening of synthetic drug libraries
US5512462A (en) * 1994-02-25 1996-04-30 Hoffmann-La Roche Inc. Methods and reagents for the polymerase chain reaction amplification of long DNA sequences
US5688997A (en) * 1994-05-06 1997-11-18 Pharmacopeia, Inc. Process for preparing intermediates for a combinatorial dihydrobenzopyran library
US5525735A (en) * 1994-06-22 1996-06-11 Affymax Technologies Nv Methods for synthesizing diverse collections of pyrrolidine compounds
US5549974A (en) * 1994-06-23 1996-08-27 Affymax Technologies Nv Methods for the solid phase synthesis of thiazolidinones, metathiazanones, and derivatives thereof
US5463564A (en) * 1994-09-16 1995-10-31 3-Dimensional Pharmaceuticals, Inc. System and method of automatically generating chemical compounds with desired properties
US5688696A (en) * 1994-12-12 1997-11-18 Selectide Corporation Combinatorial libraries having a predetermined frequency of each species of test compound
US5565974A (en) * 1995-03-31 1996-10-15 Xerox Corporation Penta-level xerographic unit
SE504798C2 (sv) * 1995-06-16 1997-04-28 Ulf Landegren Immunanalys och testkit med två reagens som kan tvärbindas om de adsorberats till analyten
WO1997000887A1 (en) * 1995-06-21 1997-01-09 Martek Biosciences Corporation Combinatorial libraries of labeled biochemical compounds and methods for producing same
US5731423A (en) * 1996-03-21 1998-03-24 Transcell Technologies, Inc. Process for preparing sulfoxides
US6117635A (en) * 1996-07-16 2000-09-12 Intergen Company Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
GB9703369D0 (en) 1997-02-18 1997-04-09 Lindqvist Bjorn H Process
WO1998054312A1 (en) 1997-05-28 1998-12-03 Babraham Institute Ribosome complexes as selection particles for in vitro display and evolution of proteins
US5989823A (en) * 1998-09-18 1999-11-23 Nexstar Pharmaceuticals, Inc. Homogeneous detection of a target through nucleic acid ligand-ligand beacon interaction
EP0930370B1 (de) * 1997-12-15 2007-08-08 CSL Behring GmbH Markierter Primer, geeignet für die Detektion von Nukleinsäuren
AU3601599A (en) 1998-03-25 1999-10-18 Ulf Landegren Rolling circle replication of padlock probes
GB9812768D0 (en) * 1998-06-13 1998-08-12 Zeneca Ltd Methods
US7306904B2 (en) * 2000-02-18 2007-12-11 Olink Ab Methods and kits for proximity probing
SE516272C2 (sv) * 2000-02-18 2001-12-10 Ulf Landegren Metoder och kit för analytdetektion mha proximitets-probning
US6511809B2 (en) * 2000-06-13 2003-01-28 E. I. Du Pont De Nemours And Company Method for the detection of an analyte by means of a nucleic acid reporter
US6531283B1 (en) 2000-06-20 2003-03-11 Molecular Staging, Inc. Protein expression profiling
DE60131903T2 (de) * 2000-10-24 2008-11-27 The Board of Trustees of the Leland S. Stanford Junior University, Palo Alto Direkte multiplex charakterisierung von genomischer dna
EP1249500A1 (de) 2001-04-12 2002-10-16 chimera biotec GmbH Verfahren zum Nachweis einer Substanz
JP2005509444A (ja) 2001-11-23 2005-04-14 シモン・フレデリックソン 多価近接プローブにより近接プロービングするための方法およびキット
US7914987B2 (en) * 2004-06-14 2011-03-29 The Board Of Trustees Of The Leland Stanford Junior University Methods and compositions for use in analyte detection using proximity probes
EP1945813A4 (de) * 2005-10-11 2010-04-21 Stratagene California Binäre signalnachweistests
WO2009012220A2 (en) 2007-07-17 2009-01-22 Agilent Technologies Inc. Analyte detection assays

Also Published As

Publication number Publication date
GB0605584D0 (en) 2006-04-26
JP5144639B2 (ja) 2013-02-13
WO2007107743A1 (en) 2007-09-27
US8268554B2 (en) 2012-09-18
EP1996941A1 (de) 2008-12-03
DE602007004666D1 (de) 2010-03-25
EP1996941B1 (de) 2010-02-03
US20100021890A1 (en) 2010-01-28
JP2009529904A (ja) 2009-08-27

Similar Documents

Publication Publication Date Title
ATE457065T1 (de) Verfahren zum analytennachweis unter verwendung von proximitätssonden
GB201101621D0 (en) Method and product
WO2005123963A3 (en) Methods and compositions for use in analyte detection using proximity probes
ATE402269T1 (de) Methoden und testsätze für annäherungstest
ATE509274T1 (de) Biosensorvorrichtung und verfahren zu ihrer verwendung
BR0014468A (pt) Processo de sequenciamento de adn
ATE426681T1 (de) Mittel und verfahren zum nachweis von zielnukleotidsequenzen unter verwendung von ligationstests mit verbesserten oligonukleotidsondenpaaren
ATE394677T1 (de) Fret proben und verfahren zur erkennung aufeinander einwirkeneden moleküle
EP2179290A4 (de) Analytnachweistests
ATE425266T1 (de) Aptamer-nanopartikelkonjugate und verwendungsverfahren zur detektion von zielanalyten
RU2009101049A (ru) Повышенная специфичность детектирования анализируемого вещества путем измерения связанных и не связанных меток
DE50014937D1 (de) Verfahren zur schnellen bestimmung von mikrobieller dns/rns, kit dafür und verwendung des verfahrens
ATE489482T1 (de) Verfahren zur herstellung von hybridsubstraten mit dna und antikörpern sowie verwendungen davon
ATE501280T1 (de) Verfahren zum nachweis eines analyten in einer biologischen matrix
GB201201547D0 (en) Method and product
ATE529533T1 (de) Verfahren zum nachweis von analyten in einer probe
ATE546550T1 (de) Verfahren zum nachweis von hpv sowie sonden, primer und kits
ATE553386T1 (de) Bluttypisierung
ATE529746T1 (de) Zusammensetzung und verfahren zum nachweis von analyten über eine polymerisierungsbasierte signalverstärkung
ATE506455T1 (de) Verfahren zum nachweis von mit lebensfähigkeit assoziierten molekülen
DE502006004013D1 (de) Verfahren zur markierung und analyse von nukleinsäuren
WO2009057702A1 (ja) 標的物質検出用ポリマー及び標的物質の検出方法
WO2009022683A1 (ja) 標的物質の検出方法
SE0602683L (sv) Diagnostisk metod
WO2009054320A1 (ja) 核酸プローブ及びプローブポリマーの形成方法

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties