BRPI0810120A2 - Processo para selecionar rapidamente hospedeiros microbianos para a identificação de certas cepas com maior rendimento e/ou qualidade na expressão de proteínas heterólogas - Google Patents

Processo para selecionar rapidamente hospedeiros microbianos para a identificação de certas cepas com maior rendimento e/ou qualidade na expressão de proteínas heterólogas

Info

Publication number
BRPI0810120A2
BRPI0810120A2 BRPI0810120-5A2A BRPI0810120A BRPI0810120A2 BR PI0810120 A2 BRPI0810120 A2 BR PI0810120A2 BR PI0810120 A BRPI0810120 A BR PI0810120A BR PI0810120 A2 BRPI0810120 A2 BR PI0810120A2
Authority
BR
Brazil
Prior art keywords
microbian
host
expression
identification
quality
Prior art date
Application number
BRPI0810120-5A2A
Other languages
English (en)
Inventor
Thomas M Ramseier
Russell J Coleman
Jane C Schneider
Charles D Hershberger
Original Assignee
Dow Global Technologies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dow Global Technologies Inc filed Critical Dow Global Technologies Inc
Publication of BRPI0810120A2 publication Critical patent/BRPI0810120A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/78—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Pseudomonas

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
BRPI0810120-5A2A 2007-04-27 2008-04-25 Processo para selecionar rapidamente hospedeiros microbianos para a identificação de certas cepas com maior rendimento e/ou qualidade na expressão de proteínas heterólogas BRPI0810120A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US91436107P 2007-04-27 2007-04-27
PCT/US2008/061483 WO2008134461A2 (en) 2007-04-27 2008-04-25 Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins

Publications (1)

Publication Number Publication Date
BRPI0810120A2 true BRPI0810120A2 (pt) 2014-11-11

Family

ID=39739280

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0810120-5A2A BRPI0810120A2 (pt) 2007-04-27 2008-04-25 Processo para selecionar rapidamente hospedeiros microbianos para a identificação de certas cepas com maior rendimento e/ou qualidade na expressão de proteínas heterólogas

Country Status (10)

Country Link
US (1) US9394571B2 (pt)
EP (2) EP2615172A1 (pt)
JP (1) JP5444553B2 (pt)
KR (1) KR101530395B1 (pt)
CN (1) CN101688213A (pt)
AU (1) AU2008245696B2 (pt)
BR (1) BRPI0810120A2 (pt)
CA (2) CA2964910C (pt)
MX (1) MX2009011523A (pt)
WO (1) WO2008134461A2 (pt)

Families Citing this family (49)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9453251B2 (en) 2002-10-08 2016-09-27 Pfenex Inc. Expression of mammalian proteins in Pseudomonas fluorescens
BRPI0513826A2 (pt) 2004-07-26 2010-06-22 Dow Global Technologies Inc processo para expressão de proteìna melhorada através de engenharia de cepa
EP2468869B1 (en) * 2007-01-31 2015-03-18 Pfenex Inc. Bacterial leader sequences for increased expression
US9394571B2 (en) 2007-04-27 2016-07-19 Pfenex Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
US9580719B2 (en) 2007-04-27 2017-02-28 Pfenex, Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
WO2010008764A1 (en) * 2008-06-23 2010-01-21 Dow Global Technologies Inc. Pseudomonas fluorescens strains for production of extracellular recombinant protein
US8524220B1 (en) 2010-02-09 2013-09-03 David Gordon Bermudes Protease inhibitor: protease sensitivity expression system composition and methods improving the therapeutic activity and specificity of proteins delivered by bacteria
US8771669B1 (en) 2010-02-09 2014-07-08 David Gordon Bermudes Immunization and/or treatment of parasites and infectious agents by live bacteria
US9597379B1 (en) 2010-02-09 2017-03-21 David Gordon Bermudes Protease inhibitor combination with therapeutic proteins including antibodies
EP2542574B1 (en) 2010-03-04 2017-08-09 Pfenex Inc. Method for producing soluble recombinant interferon protein without denaturing
KR20130072201A (ko) * 2010-03-30 2013-07-01 피페넥스 인크. 재조합 독소 단백질의 고수준 발현
AU2010201410B2 (en) * 2010-03-30 2015-04-30 Pelican Technology Holdings, Inc. High level expression of recombinant CRM197
AU2011235210B2 (en) * 2010-04-01 2015-07-16 Pfenex Inc. Methods for G-CSF production in a Pseudomonas host cell
US9169304B2 (en) 2012-05-01 2015-10-27 Pfenex Inc. Process for purifying recombinant Plasmodium falciparum circumsporozoite protein
WO2014011662A2 (en) * 2012-07-09 2014-01-16 Alvine Pharmaceuticals, Inc. Acid stable prolyl endopeptidases for degrading gluten
CA2894511C (en) 2012-12-11 2021-12-07 Albert Einstein College Of Medicine Of Yeshiva University Methods for high throughput receptor:ligand identification
US20140368723A1 (en) * 2013-06-18 2014-12-18 Samsung Electro-Mechanics Co., Ltd. Lens module and camera module including the same
US9737592B1 (en) 2014-02-14 2017-08-22 David Gordon Bermudes Topical and orally administered protease inhibitors and bacterial vectors for the treatment of disorders and methods of treatment
CN107532190B (zh) 2014-12-01 2021-07-09 菲尼克斯公司 用于肽生产的融合伴侣
US10676723B2 (en) 2015-05-11 2020-06-09 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
WO2017201210A1 (en) 2016-05-18 2017-11-23 Cue Biopharma, Inc. T-cell modulatory multimeric polypeptides and methods of use thereof
CN109689096A (zh) 2016-05-18 2019-04-26 阿尔伯特爱因斯坦医学院公司 变体pd-l1多肽、t细胞调节性多聚体多肽及其使用方法
MD3290436T2 (ro) 2016-09-01 2019-10-31 Metax Inst Fur Diatetik Gmbh Proteină fără fenilalanină pentru tratamentul fenilcetonuriei
EP3532600A4 (en) * 2016-10-19 2020-09-16 General Automation LAB Technologies Inc. HIGH RESOLUTION SYSTEMS, KITS, DEVICES AND METHODS FOR SCREENING MICRO-ORGANISMS AND OTHER HIGH-PERFORMANCE MICROBIOLOGICAL APPLICATIONS
US11180535B1 (en) 2016-12-07 2021-11-23 David Gordon Bermudes Saccharide binding, tumor penetration, and cytotoxic antitumor chimeric peptides from therapeutic bacteria
US11129906B1 (en) 2016-12-07 2021-09-28 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
ES2973548T3 (es) 2016-12-22 2024-06-20 Cue Biopharma Inc Polipéptidos multiméricos moduladores de linfocitos T y métodos para su uso
EP3565829A4 (en) 2017-01-09 2021-01-27 Cue Biopharma, Inc. T-CELL-MODULATING MULTIMERIC POLYPEPTIDES AND METHOD OF USING THEREOF
US20200010528A1 (en) 2017-03-15 2020-01-09 Cue Biopharma, Inc. Methods for modulating an immune response
US20200168291A1 (en) * 2017-06-06 2020-05-28 Zymergen Inc. Prioritization of genetic modifications to increase throughput of phenotypic optimization
WO2018226810A1 (en) * 2017-06-06 2018-12-13 Zymergen Inc. High throughput transposon mutagenesis
CA3077393A1 (en) 2017-10-27 2019-05-02 Pfenex Inc. Bacterial leader sequences for periplasmic protein expression
JP7784804B2 (ja) * 2017-10-27 2025-12-12 フェネックス インク． 組み換えｅｒｗｉｎｉａアスパラギナーゼの製造のための方法
EP3701036A4 (en) * 2017-10-27 2021-09-01 Pfenex Inc. PROCESS FOR PRODUCING ASPARAGINASE OF E. COLI
IL275239B2 (en) 2017-12-11 2026-03-01 Abalone Bio Inc Yeast presentation of proteins in the subplasmic space
CN108251330B (zh) * 2017-12-28 2021-03-23 江苏世邦生物工程科技有限公司 用于治理土壤污染的复合微生物菌剂及其制备方法和应用
CN111886241A (zh) 2018-01-09 2020-11-03 库尔生物制药有限公司 多聚体t细胞调节多肽及其使用方法
AU2020206751A1 (en) * 2019-01-07 2021-07-01 Pivot Bio, Inc. Plant colonization assays using natural microbial barcodes
CN109825464B (zh) * 2019-01-17 2021-06-15 浙江海洋大学 敲除t6ss-1基因簇的杀香鱼假单胞菌鱼用减毒疫苗
CN110041139B (zh) * 2019-05-30 2021-11-30 沈阳农业大学 一种用于连作土壤修复的生物菌多层带剂及使用方法
WO2021025043A1 (ja) * 2019-08-07 2021-02-11 学校法人東京歯科大学 判別方法、蛍光測定装置および検査薬
US12285437B2 (en) 2019-10-30 2025-04-29 The Research Foundation For The State University Of New York Reversing the undesirable pH-profile of doxorubicin via activation of a disubstituted maleamic acid prodrug at tumor acidity
CN110898080B (zh) * 2019-11-01 2021-07-06 广西中医药大学 鲎血浆在促生长发育中的应用
WO2021231376A2 (en) 2020-05-12 2021-11-18 Cue Biopharma, Inc. Multimeric t-cell modulatory polypeptides and methods of use thereof
WO2022056014A1 (en) 2020-09-09 2022-03-17 Cue Biopharma, Inc. Mhc class ii t-cell modulatory multimeric polypeptides for treating type 1 diabetes mellitus (t1d) and methods of use thereof
WO2022211829A1 (en) 2021-03-30 2022-10-06 Jazz Pharmaceuticals Ireland Ltd. Dosing of recombinant l-asparaginase
CN115725476B (zh) * 2022-12-21 2025-02-18 中国科学院沈阳应用生态研究所 一株边缘假单胞菌及其应用
CN115838712B (zh) * 2022-12-27 2024-05-14 华熙生物科技股份有限公司 具有肌肽水解酶功能的蛋白酶及其在l-肌肽合成中的应用
WO2024186259A1 (en) * 2023-03-07 2024-09-12 Karyolaimos Alexandros A method for enhancing recombinant production in a production host, an expression system, a library, a platform system, a production strain, an e. coli strain, a kit of parts, a method of using the platform system and a computer program, related to the method

Family Cites Families (305)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6696561B1 (en) 1909-07-09 2004-02-24 Basf Aktiengesellschaft Corynebacterium glutamicum genes encoding proteins involved in membrane synthesis and membrane transport
GB1381306A (en) 1972-03-03 1975-01-22 Ici Ltd Separating bacterial cells from a liquid medium
US3844893A (en) 1973-02-12 1974-10-29 Phillips Petroleum Co Microbial synthesis
US4169010A (en) 1976-11-18 1979-09-25 Phillips Petroleum Company Fermentation process for improved oxygen utilization
US4551433A (en) 1981-05-18 1985-11-05 Genentech, Inc. Microbial hybrid promoters
US5399684A (en) 1982-05-20 1995-03-21 Washington Research Foundation DNA sequences expressing mammalian alpha-1-antitrypsin
US4595658A (en) 1982-09-13 1986-06-17 The Rockefeller University Method for facilitating externalization of proteins synthesized in bacteria
US5151350A (en) 1982-10-27 1992-09-29 Repligen Corporation Cloned genes encoding recombinant protein a
US4432895A (en) 1982-11-24 1984-02-21 Hoffmann-La Roche Inc. Monomeric interferons
US4511503A (en) 1982-12-22 1985-04-16 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
GB8308483D0 (en) 1983-03-28 1983-05-05 Health Lab Service Board Secretion of gene products
US4755465A (en) 1983-04-25 1988-07-05 Genentech, Inc. Secretion of correctly processed human growth hormone in E. coli and Pseudomonas
US4680264A (en) 1983-07-01 1987-07-14 Lubrizol Genetics, Inc. Class II mobilizable gram-negative plasmid
US5281532A (en) 1983-07-27 1994-01-25 Mycogen Corporation Pseudomas hosts transformed with bacillus endotoxin genes
US4637980A (en) 1983-08-09 1987-01-20 Smithkline Beckman Corporation Externalization of products of bacteria
EP0155189A3 (en) 1984-03-16 1987-09-16 Genentech, Inc. Expression of cell wall degrading proteins and host cells harboring dna encoding such protein
SU1364343A1 (ru) 1984-07-13 1988-01-07 Всесоюзный научно-исследовательский институт генетики и селекции промышленных микроорганизмов Способ получени человеческого лейкоцитарного интерферона альфа-2
US4963495A (en) 1984-10-05 1990-10-16 Genentech, Inc. Secretion of heterologous proteins
EP0177343B1 (en) 1984-10-05 1992-07-22 Genentech, Inc. Dna, cell cultures and methods for the secretion of heterologous proteins and periplasmic protein recovery
US4695462A (en) 1985-06-28 1987-09-22 Mycogen Corporation Cellular encapsulation of biological pesticides
US4695455A (en) 1985-01-22 1987-09-22 Mycogen Corporation Cellular encapsulation of pesticides produced by expression of heterologous genes
GB8517071D0 (en) 1985-07-05 1985-08-14 Hoffmann La Roche Gram-positive expression control sequence
US4888274A (en) 1985-09-18 1989-12-19 Yale University RecA nucleoprotein filament and methods
US5618920A (en) 1985-11-01 1997-04-08 Xoma Corporation Modular assembly of antibody genes, antibodies prepared thereby and use
GB8529014D0 (en) 1985-11-25 1986-01-02 Biogen Nv Enhanced secretion of heterologous proteins
CA1338138C (en) 1986-03-24 1996-03-12 Michael A. Capage Recombinant-dna mediated production of xanthan gum
EP0380470B1 (en) 1986-03-24 1995-02-15 Texaco Development Corporation Process for the synthesis of sugar nucleotides using recombinant-dna methods
US5559015A (en) 1986-03-26 1996-09-24 Getty Scientific Development Company Recombinant-DNA mediated production of xanthan gum
US5232840A (en) 1986-03-27 1993-08-03 Monsanto Company Enhanced protein production in bacteria by employing a novel ribosome binding site
US5292507A (en) 1986-08-01 1994-03-08 Imperial Oil Limited Method of using polysaccharides to stabilize microorganisms for inoculating plant seeds
US5165927A (en) 1986-08-01 1992-11-24 University Of Southern California Composition with modified pertussis toxin
US5541308A (en) 1986-11-24 1996-07-30 Gen-Probe Incorporated Nucleic acid probes for detection and/or quantitation of non-viral organisms
US5084559A (en) 1987-03-27 1992-01-28 Repligen Corporation Protein a domain mutants
EP0288451B1 (en) 1987-04-23 1994-08-10 Monsanto Company Secretion of insulin-like growth factor 1 in E. coli
US5591603A (en) 1987-08-28 1997-01-07 Novo Nordisk A/S Process for preparing aprotinin and aprotinin analogs in yeast cells
KR0168039B1 (ko) 1987-09-04 1999-01-15 로버트 디. 웨스트 재조합 dna에서 유도한 보르데텔라 외독소 소단위체의 유사체 및 그를 포함하는 백신
GB8727489D0 (en) 1987-11-24 1987-12-23 Connaught Lab Detoxification of pertussis toxin
US5128130A (en) 1988-01-22 1992-07-07 Mycogen Corporation Hybrid Bacillus thuringiensis gene, plasmid and transformed Pseudomonas fluorescens
US5055294A (en) 1988-03-03 1991-10-08 Mycogen Corporation Chimeric bacillus thuringiensis crystal protein gene comprising hd-73 and berliner 1715 toxin genes, transformed and expressed in pseudomonas fluorescens
JPH01257491A (ja) 1988-04-08 1989-10-13 Tosoh Corp 不溶性融合異種蛋白質の処理方法
EP0422049A4 (en) 1988-05-09 1991-11-13 Us Health Vector for secretion of proteins directly into periplasm or culture medium
US5169772A (en) 1988-06-06 1992-12-08 Massachusetts Institute Of Technology Large scale method for purification of high purity heparinase from flavobacterium heparinum
WO1990003438A1 (en) 1988-09-30 1990-04-05 Allied-Signal Inc. Improved bacterial strains for heterologous gene expression
US6780613B1 (en) 1988-10-28 2004-08-24 Genentech, Inc. Growth hormone variants
US5552302A (en) 1989-04-24 1996-09-03 Promega Corporation Methods and compositions for production of human recombinant placental ribonuclease inhibitor
US6551784B2 (en) 1989-06-07 2003-04-22 Affymetrix Inc Method of comparing nucleic acid sequences
US5143854A (en) 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
US6040138A (en) 1995-09-15 2000-03-21 Affymetrix, Inc. Expression monitoring by hybridization to high density oligonucleotide arrays
US5744101A (en) 1989-06-07 1998-04-28 Affymax Technologies N.V. Photolabile nucleoside protecting groups
DE3920358A1 (de) 1989-06-22 1991-01-17 Behringwerke Ag Bispezifische und oligospezifische, mono- und oligovalente antikoerperkonstrukte, ihre herstellung und verwendung
US5683888A (en) 1989-07-22 1997-11-04 University Of Wales College Of Medicine Modified bioluminescent proteins and their use
US5169760A (en) 1989-07-27 1992-12-08 Mycogen Corporation Method, vectors, and host cells for the control of expression of heterologous genes from lac operated promoters
US5023171A (en) 1989-08-10 1991-06-11 Mayo Foundation For Medical Education And Research Method for gene splicing by overlap extension using the polymerase chain reaction
DE69002817T2 (de) 1989-11-28 1993-12-09 Wellcome Found Vakzine.
US5292658A (en) 1989-12-29 1994-03-08 University Of Georgia Research Foundation, Inc. Boyd Graduate Studies Research Center Cloning and expressions of Renilla luciferase
US5641671A (en) 1990-07-06 1997-06-24 Unilever Patent Holdings B.V. Production of active Pseudomonas glumae lipase in homologous or heterologous hosts
US5639949A (en) 1990-08-20 1997-06-17 Ciba-Geigy Corporation Genes for the synthesis of antipathogenic substances
US5662898A (en) 1990-08-20 1997-09-02 Ciba-Geigy Corporation Genes for the synthesis of antipathogenic substances
US5670350A (en) 1990-08-20 1997-09-23 Novartis Finance Corporation Genomic DNA encoding a pseudomonas global transcriptional activation element and its use in activating gene expression
US5610044A (en) 1990-10-01 1997-03-11 Lam; Stephen T. Microorganisms with mannopine catabolizing ability
JP2679390B2 (ja) 1990-10-12 1997-11-19 日本電気株式会社 コード設定回路
US5264365A (en) 1990-11-09 1993-11-23 Board Of Regents, The University Of Texas System Protease-deficient bacterial strains for production of proteolytically sensitive polypeptides
US5508192A (en) 1990-11-09 1996-04-16 Board Of Regents, The University Of Texas System Bacterial host strains for producing proteolytically sensitive polypeptides
EP0834575B1 (en) 1990-12-06 2001-11-28 Affymetrix, Inc. (a Delaware Corporation) Identification of nucleic acids in samples
ES2142801T3 (es) 1991-03-11 2000-05-01 Univ Georgia Res Found Clonacion y expresion de luciferasa de renilla.
US6800738B1 (en) 1991-06-14 2004-10-05 Genentech, Inc. Method for making humanized antibodies
US5558862A (en) 1991-09-23 1996-09-24 Monsanto Company Method of controlling insects
US5348867A (en) 1991-11-15 1994-09-20 George Georgiou Expression of proteins on bacterial surface
ATE207080T1 (de) 1991-11-25 2001-11-15 Enzon Inc Multivalente antigen-bindende proteine
IL104419A0 (en) 1992-01-24 1993-05-13 Fmc Corp Insecticidally effective peptides
GB9206645D0 (en) 1992-03-26 1992-05-06 Mini Agriculture & Fisheries Biological control of post harvest pests
DK154292D0 (da) 1992-12-23 1992-12-23 Novo Nordisk As Nyt enzym
US5652128A (en) 1993-01-05 1997-07-29 Jarvik; Jonathan Wallace Method for producing tagged genes, transcripts, and proteins
US5581476A (en) 1993-01-28 1996-12-03 Amgen Inc. Computer-based methods and articles of manufacture for preparing G-CSF analogs
GB9314886D0 (en) 1993-07-19 1993-09-01 Zeneca Ltd Production of a biological control agent
US5914254A (en) 1993-08-02 1999-06-22 Celtrix Pharmaceuticals, Inc. Expression of fusion polypeptides transported out of the cytoplasm without leader sequences
EP0759170B1 (en) 1993-09-10 2008-07-09 The Trustees Of Columbia University In The City Of New York Uses of green fluorescent protein
TW300879B (pt) 1993-11-10 1997-03-21 Ibm
WO1995015388A1 (en) 1993-12-03 1995-06-08 Medical Research Council Recombinant binding proteins and peptides
WO1995018634A1 (en) 1994-01-04 1995-07-13 The Scripps Research Institute Human monoclonal antibodies to herpes simplex virus and methods therefor
WO1995021191A1 (en) 1994-02-04 1995-08-10 William Ward Bioluminescent indicator based upon the expression of a gene for a modified green-fluorescent protein
US7294331B2 (en) 1994-03-07 2007-11-13 Immunex Corporation Methods of using flt3-ligand in hematopoietic cell transplantation
AUPM516994A0 (en) 1994-04-20 1994-05-12 Gene Shears Pty. Limited An in vivo gene expression system
US5527883A (en) 1994-05-06 1996-06-18 Mycogen Corporation Delta-endotoxin expression in pseudomonas fluorescens
US6117670A (en) 1994-06-08 2000-09-12 Novartis Finance Corporation Pyrrolnitrin biosynthesis genes and uses thereof
CA2150526C (en) 1994-06-09 2005-11-15 Andreas Kiener Biotechnological process for the preparation of cyclic s-.alpha.-amino carboxylic acids and r-.alpha.-amino carboxamides
US5989808A (en) 1994-06-14 1999-11-23 American Cyanamid Company Identification of compounds affecting specific interaction of peptide binding pairs
DE69534166T2 (de) 1994-10-28 2006-03-09 Trustees Of The University Of Pennsylvania Rekombinanter adenovirus und methoden zu dessen verwendung
US5625048A (en) 1994-11-10 1997-04-29 The Regents Of The University Of California Modified green fluorescent proteins
US5777079A (en) 1994-11-10 1998-07-07 The Regents Of The University Of California Modified green fluorescent proteins
EP0797671B1 (en) 1994-12-09 2004-03-03 Novo Nordisk A/S A process of producing extracellular proteins in bacteria
US5958713A (en) 1995-01-31 1999-09-28 Novo Nordisk A/S Method of detecting biologically active substances by using green fluorescent protein
US5641870A (en) 1995-04-20 1997-06-24 Genentech, Inc. Low pH hydrophobic interaction chromatography for antibody purification
ZA963530B (en) 1995-05-05 1996-11-05 Hoffmann La Roche Recombinant obese (ob) proteins
US5711945A (en) 1995-05-11 1998-01-27 Regents Of The University Of Minnesota Pitch degradation with Pseudomonas fluorescens
FR2734842B1 (fr) 1995-06-02 1998-02-27 Rhone Poulenc Agrochimie Sequence adn d'un gene de l'hydroxy-phenyl pyruvate dioxygenase et obtention de plantes contenant un gene de l'hydroxy-phenyl pyruvate dioxygenase, tolerantes a certains herbicides
US6083690A (en) 1995-06-02 2000-07-04 Osteoscreen, Inc. Methods and compositions for identifying osteogenic agents
US5955298A (en) 1995-06-26 1999-09-21 The United States Of America As Represented By The Secretary Of Agriculture Sequences for production of 2,4-Diacetylphloroglucinol and methods
US6495357B1 (en) 1995-07-14 2002-12-17 Novozyme A/S Lipolytic enzymes
US6020473A (en) 1995-08-25 2000-02-01 Genentech, Inc. Nucleic acids encoding variants of vascular endothelial cell growth factor
ATE184613T1 (de) 1995-09-22 1999-10-15 Novo Nordisk As Varianten des grünen fluoreszenzproteins, gfp
US5952236A (en) 1995-10-26 1999-09-14 Thompson; Richard B. Enzyme-based fluorescence biosensor for chemical analysis
US5968738A (en) 1995-12-06 1999-10-19 The Board Of Trustees Of The Leland Stanford Junior University Two-reporter FACS analysis of mammalian cells using green fluorescent proteins
US5776730A (en) 1995-12-15 1998-07-07 University Of Hawaii Neurospora hosts for the production of recombinant proteins, and methods for producing same
US6020192A (en) 1996-01-18 2000-02-01 University Of Florida Humanized green fluorescent protein genes and methods
US5874304A (en) 1996-01-18 1999-02-23 University Of Florida Research Foundation, Inc. Humanized green fluorescent protein genes and methods
EP0880598A4 (en) 1996-01-23 2005-02-23 Affymetrix Inc RAPID EVALUATION OF NUCLEIC ACID ABUNDANCE DIFFERENCE, WITH A HIGH-DENSITY OLIGONUCLEOTIDE SYSTEM
US5804387A (en) 1996-02-01 1998-09-08 The Board Of Trustees Of The Leland Stanford Junior University FACS-optimized mutants of the green fluorescent protein (GFP)
US5876995A (en) 1996-02-06 1999-03-02 Bryan; Bruce Bioluminescent novelty items
US6255558B1 (en) 1996-02-14 2001-07-03 Btg International Limited Gene expression
US6121247A (en) 1996-03-29 2000-09-19 The Johns Hopkins University Therapy for allergic diseases
US5741663A (en) 1996-04-16 1998-04-21 University Of Georgia Research Foundation, Inc. Selective additive and media for Pseudomonas fluorescens
US6218185B1 (en) 1996-04-19 2001-04-17 The United States Of America As Represented By The Secretary Of Agriculture Piggybac transposon-based genetic transformation system for insects
US6699838B1 (en) 1996-05-03 2004-03-02 Abbott Laboratories Antiangiogenic peptides
US6096865A (en) 1996-05-06 2000-08-01 Amgen Inc. Mutants of the green fluorescent protein having improved fluorescent properties at 37°
US6027881A (en) 1996-05-08 2000-02-22 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Mutant Aequorea victoria fluorescent proteins having increased cellular fluorescence
US5948889A (en) 1996-05-21 1999-09-07 Case Western Reserve University Compositions and methods for screening antimicrobials
US5925558A (en) 1996-07-16 1999-07-20 The Regents Of The University Of California Assays for protein kinases using fluorescent protein substrates
US5912137A (en) 1996-07-16 1999-06-15 The Regents Of The University Of California Assays for protein kinases using fluorescent
US7129061B1 (en) 1996-08-07 2006-10-31 Biogen Idec Ma Inc. Tumor necrosis factor related ligand
US5948681A (en) 1996-08-14 1999-09-07 Children's Hospital Of Philadelphia Non-viral vehicles for use in gene transfer
US6124128A (en) 1996-08-16 2000-09-26 The Regents Of The University Of California Long wavelength engineered fluorescent proteins
US5914233A (en) 1996-08-23 1999-06-22 Osteo Screen Screening assay for the identification of agents which alter expression of PTH-rP
WO1998008839A1 (en) 1996-08-26 1998-03-05 Eli Lilly And Company Combinatorial process for preparing substituted thiophene libraries
AU722375B2 (en) 1996-09-06 2000-08-03 Trustees Of The University Of Pennsylvania, The Methods using cre-lox for production of recombinant adeno-associated viruses
US6025192A (en) 1996-09-20 2000-02-15 Cold Spring Harbor Laboratory Modified retroviral vectors
US6255071B1 (en) 1996-09-20 2001-07-03 Cold Spring Harbor Laboratory Mammalian viral vectors and their uses
US6136566A (en) 1996-10-04 2000-10-24 Lexicon Graphics Incorporated Indexed library of cells containing genomic modifications and methods of making and utilizing the same
US5976796A (en) 1996-10-04 1999-11-02 Loma Linda University Construction and expression of renilla luciferase and green fluorescent protein fusion genes
TW371617B (en) 1996-10-09 1999-10-11 Of Animal And Plant Health Inspection And Quarantine Council Of Agriculture Executive Yuan Bureau Method to transplant GFP into autographa californica multiple nuclear polyhedrosis virus for inflicting pest in an attempt to detect and flow up it existence and to improve life span against UV
US5757051A (en) 1996-11-12 1998-05-26 Micron Technology, Inc. Static memory cell and method of manufacturing a static memory cell
CA2272578A1 (en) 1996-11-18 1998-05-28 Mcgill University Post-mitotic neurons containing adenovirus vectors that modulate apoptosis and growth
EP0941350A1 (en) 1996-12-06 1999-09-15 Novartis AG GENETICALLY MODIFIED $i(PSEUDOMONAS) STRAINS WITH ENHANCED BIOCONTROL ACTIVITY
US5891688A (en) 1996-12-06 1999-04-06 Novartis Finance Corporation DNA encoding lemA-independent GacA and its use in activating gene expression
US5955348A (en) 1997-11-25 1999-09-21 Novartis Ag Genetically modified pseudomonas strains with enhanced biocontrol activity
US5756087A (en) 1996-12-06 1998-05-26 Novartis Finance Corporation Genetically modified Pseudomonas strains with enhanced biocontrol activity
IL129767A0 (en) 1996-12-12 2000-02-29 Prolume Ltd Apparatus and method for detecting and identifying infectious agents
US6194194B1 (en) 1996-12-23 2001-02-27 Daniel Molloy Method for controlling dreissena species
DE69740107D1 (de) 1996-12-23 2011-03-10 Immunex Corp Rezeptor aktivator von nf-kappa b, rezeptor is mitglied der tnf rezeptor superfamilie
WO1998029533A1 (en) 1996-12-31 1998-07-09 Gaitanaris George A Vectors and methods for the mutagenesis of mammalian genes
US6090919A (en) 1997-01-31 2000-07-18 The Board Of Trustees Of The Leland Stanford Junior University FACS-optimized mutants of the green fluorescent protein (GFP)
US5994077A (en) 1997-01-31 1999-11-30 The Board Of Trustees Of The Leland Stanford Junior University Flourescence-based isolation of differentially induced genes
US5795759A (en) 1997-03-10 1998-08-18 The University Of Iowa Research Foundation Carboxylic acid reductase, and methods of using same
US6197928B1 (en) 1997-03-14 2001-03-06 The Regents Of The University Of California Fluorescent protein sensors for detection of analytes
US5994071A (en) 1997-04-04 1999-11-30 Albany Medical College Assessment of prostate cancer
US5952208A (en) 1997-04-07 1999-09-14 Energy Biosystems Corporation Dsz gene expression in pseudomonas hosts
US6251384B1 (en) 1997-04-28 2001-06-26 Anticancer, Inc. Metastasis models using green fluorescent protein (GFP) as a marker
US5993778A (en) 1997-05-07 1999-11-30 Firestein; Stuart J. Functional expression of, and assay for, functional cellular receptors in vivo
US6225082B1 (en) 1997-05-09 2001-05-01 Research Corporation Technologies, Inc. Myelin basic protein MRNA transport and translation enhancer sequences
US6303373B1 (en) 1999-06-09 2001-10-16 Whitehead Institute For Biomedical Research Method of measuring plasma membrane targeting of GLUT4
US6251582B1 (en) 1997-07-17 2001-06-26 New York University Alternative G-coupled receptors associated with retroviral entry into cells, methods of identifying the same, and diagnostic and therapeutic uses thereof
US6420108B2 (en) 1998-02-09 2002-07-16 Affymetrix, Inc. Computer-aided display for comparative gene expression
US6188783B1 (en) 1997-07-25 2001-02-13 Affymetrix, Inc. Method and system for providing a probe array chip design database
IL121404A0 (en) 1997-07-27 1998-01-04 Yissum Res Dev Co Transgenic higher plants of altered structural morphology
US6221612B1 (en) 1997-08-01 2001-04-24 Aurora Biosciences Corporation Photon reducing agents for use in fluorescence assays
US6214563B1 (en) 1997-08-01 2001-04-10 Aurora Biosciences Corporation Photon reducing agents for reducing undesired light emission in assays
US6251677B1 (en) 1997-08-25 2001-06-26 The Trustees Of The University Of Pennsylvania Hybrid adenovirus-AAV virus and methods of use thereof
HUP9701446A1 (hu) 1997-08-27 1999-05-28 Phylaxia-Pharma Gyógyszer-, Oltóanyag és Agrobiológiai Készítményeket Gyártó és Forgalmazó Rt. Eljárás a talaj mikroorganizmus populációjának előnyös kialakítására
US6153409A (en) 1997-09-11 2000-11-28 University Of Maryland Biotechnology Institute Process for continuous optimized protein production in insect larvae
US6071738A (en) 1997-09-19 2000-06-06 Energy Biosystems Corporation Conversion of organosulfur compounds to oxyorganosulfur compounds for desulfurization of fossil fuels
IL135242A0 (en) 1997-09-26 2001-05-20 Athersys Inc Expression of endogenous genes by non-homologous recombination of a vector construct with cellular dna
US6130313A (en) 1997-10-02 2000-10-10 Clontech Laboratories, Inc. Rapidly degrading GFP-fusion proteins
US6133429A (en) 1997-10-03 2000-10-17 Becton Dickinson And Company Chromophores useful for the preparation of novel tandem conjugates
US6284496B1 (en) 1997-10-03 2001-09-04 University Of South Florida DNA vector for determining the presence of out-of-reading-frame mutations
US6361989B1 (en) 1997-10-13 2002-03-26 Novozymes A/S α-amylase and α-amylase variants
US5968773A (en) 1997-11-14 1999-10-19 Heddle; John A. System and method for regulation of gene expression
US6277625B1 (en) 1997-11-20 2001-08-21 The United States Of America As Represented By The Secretary Of Agriculture Transgenic strains of Pseudomonas for biocontrol of plant root diseases
US6447770B1 (en) 1997-11-20 2002-09-10 The United States Of America As Represented By The Secretary Of Agriculture Biocontrol agents for take-all
US6013447A (en) 1997-11-21 2000-01-11 Innovir Laboratories, Inc. Random intracellular method for obtaining optimally active nucleic acid molecules
JP3736617B2 (ja) 1997-12-12 2006-01-18 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア 細胞型の決定方法
US6060594A (en) 1997-12-18 2000-05-09 Ecogen, Inc. Nucleic acid segments encoding modified bacillus thuringiensis coleopteran-toxic crystal proteins
US6506559B1 (en) 1997-12-23 2003-01-14 Carnegie Institute Of Washington Genetic inhibition by double-stranded RNA
US6136539A (en) 1998-02-11 2000-10-24 The Regents Of The University Of California Compositions and methods for the inhibition of MUC-5 mucin gene expression
US6156552A (en) 1998-02-18 2000-12-05 Novo Nordisk A/S Lipase variants
US5922576A (en) 1998-02-27 1999-07-13 The John Hopkins University Simplified system for generating recombinant adenoviruses
US6210910B1 (en) 1998-03-02 2001-04-03 Trustees Of Tufts College Optical fiber biosensor array comprising cell populations confined to microcavities
US6261760B1 (en) 1998-03-09 2001-07-17 The Regents Of The University Of California Regulation of the cell cycle by sterols
AUPP249298A0 (en) 1998-03-20 1998-04-23 Ag-Gene Australia Limited Synthetic genes and genetic constructs comprising same I
JP2002507410A (ja) 1998-03-27 2002-03-12 プロルーム・リミテッド ルシフェラーゼ、蛍光タンパク質、ルシフェラーゼおよび蛍光タンパク質をコードする核酸および、診断、高処理スクリーニングおよび新規アイテムにおけるその使用
US6080576A (en) 1998-03-27 2000-06-27 Lexicon Genetics Incorporated Vectors for gene trapping and gene activation
US6248558B1 (en) 1998-03-31 2001-06-19 Vanderbilt University Sequence and method for genetic engineering of proteins with cell membrane translocating activity
WO1999053035A1 (en) 1998-04-13 1999-10-21 The University Of Georgia Research Foundation, Inc. Pyruvate carboxylase overexpression for enhanced production of oxaloacetate-derived biochemicals in microbial cells
US6037133A (en) 1998-04-17 2000-03-14 Clontech Laboratories, Inc. IκBEGFP constructs, cell lines and methods of use
US7112324B1 (en) 1998-04-21 2006-09-26 Micromet Ag CD 19×CD3 specific polypeptides and uses thereof
US6280934B1 (en) 1998-05-13 2001-08-28 Millennium Pharmaceuticals, Inc. Assay for agents which alter G-protein coupled receptor activity
US20030064435A1 (en) 1998-05-28 2003-04-03 Weiner Joel Hirsch Compositions and methods for protein secretion
US6150176A (en) 1998-06-09 2000-11-21 The Regents Of The University Of California Fluorescent protein sensors for measuring the pH of a biological sample
US6140132A (en) 1998-06-09 2000-10-31 The Regents Of The University Of California Fluorescent protein sensors for measuring the pH of a biological sample
US6291175B1 (en) 1998-06-16 2001-09-18 Variagenics, Inc. Methods for treating a neurological disease by determining BCHE genotype
US5989837A (en) 1998-07-13 1999-11-23 Wisconsin Alumni Research Foundation Immortalized human keratinocyte cell line
AUPP589798A0 (en) 1998-09-14 1998-10-08 Charles Sturt University Novel bacterial strains and methods of controlling fungal pathogens
US6185561B1 (en) 1998-09-17 2001-02-06 Affymetrix, Inc. Method and apparatus for providing and expression data mining database
US6323007B1 (en) 1998-09-18 2001-11-27 Novozymes A/S 2,6-β-D-fructan hydrolase enzyme and processes for using the enzyme
US6180343B1 (en) 1998-10-08 2001-01-30 Rigel Pharmaceuticals, Inc. Green fluorescent protein fusions with random peptides
US5985577A (en) 1998-10-14 1999-11-16 The Trustees Of Columbia University In The City Of New York Protein conjugates containing multimers of green fluorescent protein
US6203986B1 (en) 1998-10-22 2001-03-20 Robert H. Singer Visualization of RNA in living cells
US6268201B1 (en) 1998-10-23 2001-07-31 Albert Einstein College Of Medicine Of Yeshiva University IniB, iniA and iniC genes of mycobacteria and methods of use
PT1124941E (pt) 1998-10-28 2004-02-27 Genentech Inc Processo para recuperar polipeptidos heterologos a partir de celulas bacterianas
US6270958B1 (en) 1998-10-29 2001-08-07 Washington University Detection of negative-strand RNA viruses
WO2000029604A1 (en) 1998-11-18 2000-05-25 The University Of Akron Production of biological materials by simultaneous aerobic and anaerobic respiration
US6210922B1 (en) 1998-11-30 2001-04-03 National Research Council Of Canada Serum free production of recombinant proteins and adenoviral vectors
US6136538A (en) 1999-01-08 2000-10-24 Washington University Silent inducible virus replicons and uses thereof
US6015557A (en) 1999-02-24 2000-01-18 Tobinick; Edward L. Tumor necrosis factor antagonists for the treatment of neurological disorders
KR100312456B1 (ko) 1999-03-13 2001-11-03 윤덕용 슈도모나스 플루오레슨스 유래의 외래단백질 분비촉진유전자
AU779814B2 (en) 1999-03-29 2005-02-10 Novozymes A/S Polypeptides having branching enzyme activity and nucleic acids encoding same
US6960652B2 (en) 1999-03-30 2005-11-01 Board Of Regents, The University Of Texas System Compositions and methods for modifying toxic effects of proteinaceous compounds
US6664386B1 (en) 1999-04-08 2003-12-16 The Research Foundation Of State University Of New York System for efficient secretion of recombinant proteins
AU783682B2 (en) 1999-08-04 2005-11-24 Amgen, Inc. Fhm, a novel member of the TNF ligand supergene family
US6558939B1 (en) 1999-08-31 2003-05-06 Novozymes, A/S Proteases and variants thereof
US7459540B1 (en) 1999-09-07 2008-12-02 Amgen Inc. Fibroblast growth factor-like polypeptides
DE19944870A1 (de) 1999-09-18 2001-03-29 Aventis Pharma Gmbh Signalsequenzen zur Herstellung von Leu-Hirudin über Sekretion durch E. coli in das Kulturmedium
AU1206801A (en) 1999-10-13 2001-04-23 University Of Georgia Research Foundation, Inc., The High yield protein expression system and methods
US6607885B1 (en) 1999-10-15 2003-08-19 E. I. Du Pont De Nemours And Company Method for high-density microarray medicated gene expression profiling
US6617143B1 (en) 1999-10-20 2003-09-09 Novozymes A/S Polypeptides having glucanotransferase activity and nucleic acids encoding same
WO2001032844A1 (en) * 1999-11-05 2001-05-10 Novozymes A/S Microtiter plate (mtp) based high throughput screening (hts) assays
CA2390292A1 (en) 1999-11-12 2001-05-25 Maxygen Holdings Ltd. Interferon gamma conjugates
AU2001239884B2 (en) 2000-02-25 2006-08-10 Vegenics Limited Materials and methods involving hybrid vascular endothelial growth factor DNAs and proteins and screening methods for modulators
ES2336887T5 (es) 2000-03-30 2019-03-06 Whitehead Inst Biomedical Res Mediadores de interferencia por ARN específicos de secuencias de ARN
US6509181B1 (en) 2000-04-14 2003-01-21 Novozymes, A/S Polypeptides having haloperoxide activity
JP2001299360A (ja) 2000-04-28 2001-10-30 Morihiro Matsuda ヒト型抗破傷風毒素一本鎖組換え抗体フラグメント
CN1137999C (zh) 2000-07-04 2004-02-11 清华大学 集成式微阵列装置
GB0016702D0 (en) 2000-07-08 2000-08-23 Secr Defence Brit Expression system
US20030108980A1 (en) 2000-08-14 2003-06-12 Sayler Gary S. Bioluminescent methods for direct visual detection of environmental compounds
WO2002016940A2 (en) 2000-08-23 2002-02-28 Genome Therapeutics Corporation Genomics-assisted rapid identification of targets
JP2002201141A (ja) 2000-10-27 2002-07-16 Hayashibara Biochem Lab Inc 蛋白質合成抑制遺伝子の発現増強剤
US6673580B2 (en) 2000-10-27 2004-01-06 Genentech, Inc. Identification and modification of immunodominant epitopes in polypeptides
GB0027782D0 (en) 2000-11-14 2000-12-27 Boehringer Ingelheim Int Methods fro large scale protein productio in prokaryotes
US7087412B2 (en) 2000-11-14 2006-08-08 Boehringer Ingelheim International Gmbh Methods for large scale protein production in prokaryotes
JP4056880B2 (ja) 2000-12-14 2008-03-05 ジェネンテック・インコーポレーテッド 細菌性宿主株
WO2002061090A2 (en) 2000-12-14 2002-08-08 Genentech, Inc. Prokaryotically produced antibodies and uses thereof
US6979556B2 (en) 2000-12-14 2005-12-27 Genentech, Inc. Separate-cistron contructs for secretion of aglycosylated antibodies from prokaryotes
WO2002053191A1 (en) 2001-01-05 2002-07-11 Viromed Limited Compositions for gene therapy of rheumatoid arthritis including a gene encoding an anti-angiogenic protein or parts thereof
US7202059B2 (en) 2001-02-20 2007-04-10 Sanofi-Aventis Deutschland Gmbh Fusion proteins capable of being secreted into a fermentation medium
DE10108212A1 (de) 2001-02-20 2002-08-22 Aventis Pharma Gmbh Fusionsprotein zur Sekretion von Wertprotein in bakterielle Überstände
US6887462B2 (en) 2001-04-09 2005-05-03 Chiron Corporation HSA-free formulations of interferon-beta
US7217785B2 (en) 2001-05-09 2007-05-15 The Regents Of The University Of California Cysteine-containing peptides having antioxidant properties
WO2002092000A2 (en) 2001-05-11 2002-11-21 Genome Therapeutics Corporation Hbm variants that modulate bone mass and lipid levels
CA2347657A1 (en) 2001-05-22 2002-11-22 University Of British Columbia Expression of heterologous peptides from caulobacter
IL159756A0 (en) 2001-07-12 2004-06-20 Univ Massachusetts IN VIVO PRODUCTION OF SMALL INTERFERING RNAs THAT MEDIATE GENE SILENCING
WO2003012052A2 (en) 2001-07-30 2003-02-13 The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Specific inhibition of gene expression by small double stranded rnas
JP4344858B2 (ja) 2001-09-13 2009-10-14 カリフォルニア インスティチュート オブ テクノロジー 細胞内で抗ウイルス性小ｒｎａ分子を発現させる方法
US7419783B2 (en) 2001-11-05 2008-09-02 Research Development Foundation Engineering of leader peptides for the secretion of recombinant proteins in bacteria
AU2002360394A1 (en) 2001-11-16 2003-06-10 University Of Massachusetts Facilitation of rna interference
CN1620508A (zh) 2001-12-21 2005-05-25 牛津生物医学（英国）有限公司 转基因生物
US6806328B2 (en) 2002-01-28 2004-10-19 Univation Technologies, Llc Polymerization catalyst activator complexes and their use in a polymerization process
EP1572902B1 (en) 2002-02-01 2014-06-11 Life Technologies Corporation HIGH POTENCY siRNAS FOR REDUCING THE EXPRESSION OF TARGET GENES
AU2002306484A1 (en) * 2002-02-13 2003-09-04 Dow Global Technologies Inc. Over-expression of extremozyme genes in pseudomonads and closely related bacteria
EP1474433A4 (en) 2002-02-20 2005-02-23 Sirna Therapeutics Inc TARGET LOCALIZATION TARGETED BY RNA INTERFERENCE AND TARGET VALIDATION WITH SHORT INTERFERING NUCLEIC ACID (siNA)
AU2003218217A1 (en) 2002-03-15 2003-09-29 The Trustees Of The University Of Pennsylvania Heterologous protein production using the twin arginine translocation pathway
CA2482995C (en) 2002-04-22 2013-01-29 Dow Global Technologies Inc. Low-cost production of peptides
EP1506286B1 (en) 2002-05-24 2014-03-19 Merck Sharp & Dohme Corp. Neutralizing human anti-igfr antibody
US7439063B2 (en) 2002-06-11 2008-10-21 Burnham Institute For Medical Research Neuroprotective synergy of erythropoietin and insulin-like growth factors
WO2004041857A2 (en) 2002-06-17 2004-05-21 Ballard Jimmy D Mutant of clostridium difficile toxin b and methods of use
CA2491178A1 (en) 2002-07-03 2004-01-15 Maxygen Holdings Ltd. Full-length interferon gamma polypeptide variants
EP1539794B1 (en) 2002-07-03 2008-12-31 Dow Global Technologies Inc. Benzoate- and anthranilate-inducible promoters
AU2003243831B2 (en) 2002-07-11 2008-07-24 Sugar Research Australia Limited Transgenic plants used as a bioreactor system
NO20023581D0 (no) 2002-07-26 2002-07-26 Fmc Biopolymer As Nye mutantstammer av Pseudomonas fluorescens og varianter derav, metoder for produksjon og bruk derav til produksjon avalginat
US20040157289A1 (en) 2002-09-06 2004-08-12 Salerno John C. Protein expression system
US9453251B2 (en) 2002-10-08 2016-09-27 Pfenex Inc. Expression of mammalian proteins in Pseudomonas fluorescens
CA2501690C (en) 2002-10-08 2012-04-03 Frank H. Gaertner Amended recombinant cells (arcs) for the production and delivery of antiviral agents, adjuvants and vaccine accelerants
EP1900753B1 (en) 2002-11-08 2017-08-09 Ablynx N.V. Method of administering therapeutic polypeptides, and polypeptides therefor
US20060034845A1 (en) 2002-11-08 2006-02-16 Karen Silence Single domain antibodies directed against tumor necrosis factor alpha and uses therefor
EP1558645B1 (en) 2002-11-08 2011-07-27 Ablynx N.V. Stabilized single domain antibodies in pharmaceutical composition adapted for inhalation
DE10258885A1 (de) 2002-12-17 2004-07-15 Aventis Pharma Deutschland Gmbh Verfahren zur Generierung eines gentechnisch veränderten Organismus
JP2006517789A (ja) 2003-01-10 2006-08-03 アブリンクス エン．ヴェー． 治療用ポリペプチド、その相同物、その断片、および血小板媒介凝集の調節での使用
EP1599499A2 (en) 2003-02-24 2005-11-30 Dow Global Technologies Inc. Periodic antimicrobial peptides
JP2008501621A (ja) 2003-05-31 2008-01-24 マイクロメット アクツィエン ゲゼルシャフト Ｂ細胞関連疾患を処置するための二重特異性抗ｃｄ３、抗ｃｄ１９抗体構築物を含む薬学的組成物
PL1633876T3 (pl) 2003-06-17 2009-01-30 Sembiosys Genetics Inc Sposoby wytwarzania insuliny w roślinach
WO2005052151A1 (en) 2003-11-19 2005-06-09 Dow Global Technologies Inc. Improved protein expression systems
CA2546157C (en) * 2003-11-21 2014-07-22 Dow Global Technolgies Inc. Improved expression systems with sec-system secretion
EP1687016A4 (en) 2003-11-25 2009-07-29 Anjin Corp GENETIC VARIANT OF DIPHTHERIC TOXIN
CA2547511A1 (en) 2003-12-01 2005-07-28 Dow Global Technolgies Inc. Recombinant icosahedral virus like particle production in pseudomonads
EP2270163A1 (en) 2003-12-10 2011-01-05 Eli Lilly and Company Muteins of fibroblast growth factor 21
US7235641B2 (en) 2003-12-22 2007-06-26 Micromet Ag Bispecific antibodies
EP1709170B1 (en) 2004-01-16 2018-02-21 Pfenex Inc. Expression of mammalian proteins in pseudomonas fluorescens
DE102004014983A1 (de) 2004-03-26 2005-10-20 Univ Stuttgart Rekombinante Polypeptide der Mitglieder der TNF Ligandenfamilie und deren Verwendung
US7384779B2 (en) 2004-04-12 2008-06-10 Corning Incorporated Porous substrate plates and the use thereof
EP2194064A1 (en) 2004-05-13 2010-06-09 Eli Lilly & Company FGF-21 fusion proteins
JP2008511286A (ja) 2004-06-02 2008-04-17 ダイアテック・ピーティワイ・リミテッド サメ類のＩｇＮＡＲドメインに基づく結合成分
BRPI0513826A2 (pt) * 2004-07-26 2010-06-22 Dow Global Technologies Inc processo para expressão de proteìna melhorada através de engenharia de cepa
KR100854198B1 (ko) 2004-09-02 2008-08-26 일라이 릴리 앤드 캄파니 섬유아세포 성장 인자 21의 뮤테인
US7563443B2 (en) 2004-09-17 2009-07-21 Domantis Limited Monovalent anti-CD40L antibody polypeptides and compositions thereof
WO2006040153A2 (en) 2004-10-13 2006-04-20 Ablynx N.V. Single domain camelide anti -amyloid beta antibodies and polypeptides comprising the same for the treatment and diagnosis of degenarative neural diseases such as alzheimer's disease
JP4719892B2 (ja) 2004-12-03 2011-07-06 国立大学法人徳島大学 微生物または生体分子の収容容器、並びにその作成方法及び使用方法
EP1836293A2 (en) * 2004-12-17 2007-09-26 Nitto Denko Corporation Immobilized degradable cationic polymer for transfecting eukaryotic cells
US8420102B2 (en) 2006-03-07 2013-04-16 Vaxinnate Corporation Compositions that include hemagglutinin
WO2006074947A2 (en) 2005-01-14 2006-07-20 Ablynx N.V. METHODS AND ASSAYS FOR DISTINGUISHING BETWEEN DIFFERENT FORMS OF DISEASES AND DISORDERS CHARACTERIZED BY THROMBOCYTOPENIA AND/OR BY SPONTANEOUS INTERACTION BETWEEN VON WILLEBRAND FACTOR (vWF) AND PLATELETS
AU2005325801A1 (en) 2005-01-31 2006-08-03 Ablynx N.V. Method for generating variable domain sequences of heavy chain antibodies
JO3058B1 (ar) 2005-04-29 2017-03-15 Applied Molecular Evolution Inc الاجسام المضادة لمضادات -اي ال-6,تركيباتها طرقها واستعمالاتها
US8188223B2 (en) 2005-05-18 2012-05-29 Ablynx N.V. Serum albumin binding proteins
WO2007011670A2 (en) 2005-07-15 2007-01-25 Zymogenetics, Inc. Heterodimeric four helix bundle cytokines
ATE518882T1 (de) 2005-09-19 2011-08-15 Allergan Inc Mit clostridientoxin aktivierbare clostridientoxine
US20090252681A1 (en) 2005-10-11 2009-10-08 Ablynx N.V. Nanobodies and Polypeptides Against EGFR and IGF-IR
JP5399712B2 (ja) 2005-12-21 2014-01-29 アムゲン リサーチ （ミュンヘン） ゲーエムベーハー 可溶性ｃｅａに対する抵抗性を有する医薬組成物
US20090226444A1 (en) 2005-12-21 2009-09-10 Micromet Ag Pharmaceutical antibody compositions with resistance to soluble cea
US7553941B2 (en) 2006-02-03 2009-06-30 Modigene Inc Long-acting polypeptides and methods of producing same
US20070269422A1 (en) 2006-05-17 2007-11-22 Ablynx N.V. Serum albumin binding proteins with long half-lives
US9637753B2 (en) 2006-08-10 2017-05-02 Plantechno S.R.L. In-plant production of oligomeric (comprising three or more units) forms of human Apo A-1 protein muteins
ATE536369T1 (de) 2006-10-11 2011-12-15 Ablynx Nv Im wesentlichen ph-wert-unabhängigerweise an serumproteine bindende aminosäuresequenzen, verbindungen, die diese enthalten, und deren verwendung
US20080267949A1 (en) 2006-12-05 2008-10-30 Ablynx N.V. Peptides capable of binding to serum proteins
EP2468869B1 (en) 2007-01-31 2015-03-18 Pfenex Inc. Bacterial leader sequences for increased expression
US9394571B2 (en) 2007-04-27 2016-07-19 Pfenex Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
US9580719B2 (en) 2007-04-27 2017-02-28 Pfenex, Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
EP2185704A1 (en) 2007-08-03 2010-05-19 Pfenex, Inc. Translation initiation region sequences for the optimal expression of heterologous proteins
BRPI0819598A2 (pt) 2007-11-30 2017-05-09 Kalobios Pharmaceuticals Inc anticorpos para o antígeno pcrv da pseudomonas aeruginosa

Also Published As

Publication number Publication date
EP2142651A2 (en) 2010-01-13
MX2009011523A (es) 2009-11-09
CA2964910C (en) 2018-01-09
KR101530395B1 (ko) 2015-06-19
KR20100023824A (ko) 2010-03-04
CA2685326A1 (en) 2008-11-06
CN101688213A (zh) 2010-03-31
US9394571B2 (en) 2016-07-19
AU2008245696A1 (en) 2008-11-06
US20080269070A1 (en) 2008-10-30
WO2008134461A2 (en) 2008-11-06
EP2142651B1 (en) 2013-05-22
EP2615172A1 (en) 2013-07-17
JP5444553B2 (ja) 2014-03-19
JP2010524506A (ja) 2010-07-22
CA2964910A1 (en) 2008-11-06
WO2008134461A3 (en) 2008-12-24
AU2008245696B2 (en) 2013-11-07

Similar Documents

Publication Publication Date Title
BRPI0810120A2 (pt) Processo para selecionar rapidamente hospedeiros microbianos para a identificação de certas cepas com maior rendimento e/ou qualidade na expressão de proteínas heterólogas
BRPI1010759A2 (pt) processo para produção de proteína
BRPI0716139A2 (pt) processo para purificaÇço de proteÍnas de fusço fc
BRPI0809890A2 (pt) Processo para preparar imatinib ou sal do mesmo e composição farmacêutica
BRPI0814291A2 (pt) Processo para produção de alcoóis
BRPI0720070A2 (pt) Processo para produção de produtos de tecido
BRPI0915366A2 (pt) dispositivo e processo para produção de farinha e/ou sêmola
BRPI0819659A2 (pt) Processo para produção de álcool
BRPI0722077A2 (pt) Processo para o acabamento de poliolefina
BRPI0819688A2 (pt) Processo para tratamento de fratura óssea com anticorpos anti-esclerostina.
BRPI0906246A2 (pt) Processo para alisamento ou relaxamento dos cabelos e compisição para tratar os cabelos
PT2351578T (pt) Processo para o fabrico de vacinas
BRPI0816478A2 (pt) Processo para produção de celulases
BRPI0716479A2 (pt) Processo sol-gel
BRPI0909932A2 (pt) "composição de protéina hidrolisada, processo para a preparação de uma composição de proteína hidrolisada e produto alimentício"
BRPI0910454A2 (pt) processo para produção de uma proteína policlonal
BRPI0916848A2 (pt) unidade e processo de tratamentos de superfície de vidro plano.
BRPI0920920A2 (pt) processo para dessulfuração
EP1973429A4 (en) PROTEIN HYDROLYSATES AND MANUFACTURING METHOD THEREFOR
BRPI0910312A2 (pt) processo para preparação de amidas em presença de água superaquecida
BR112012010194A2 (pt) processo econômico para produção de xilose de hidrolisado usando eletrodiálise e processo de recuperação direta
BRPI0717758A2 (pt) Processo para geração de proteína e usos das mesmas
CR10905A (es) Proceso para producir biomasa y proteinas por microalgas
PT2078730E (pt) Processo para a obtenção de um concentrado do factor de von willebrand ou de um complexo factor viii/factor de von willebrand e sua utilização
BRPI0909004A2 (pt) Processo para produção de biogás

Legal Events

Date Code Title Description
B11A Dismissal acc. art.33 of ipl - examination not requested within 36 months of filing
B11Y Definitive dismissal - extension of time limit for request of examination expired [chapter 11.1.1 patent gazette]
B350 Update of information on the portal [chapter 15.35 patent gazette]