DK0832200T3 - Nye metoder til rensning af faktor IX - Google Patents

Nye metoder til rensning af faktor IX

Info

Publication number
DK0832200T3
DK0832200T3 DK96915892T DK96915892T DK0832200T3 DK 0832200 T3 DK0832200 T3 DK 0832200T3 DK 96915892 T DK96915892 T DK 96915892T DK 96915892 T DK96915892 T DK 96915892T DK 0832200 T3 DK0832200 T3 DK 0832200T3
Authority
DK
Denmark
Prior art keywords
purification
factor
new methods
hydroxyapatite
resins
Prior art date
Application number
DK96915892T
Other languages
Danish (da)
English (en)
Inventor
W Barry Foster
Robert J Costigan
Duane Bonam
Mary B Switzer
Rochelle Walsh
Original Assignee
Genetics Inst Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genetics Inst Llc filed Critical Genetics Inst Llc
Application granted granted Critical
Publication of DK0832200T3 publication Critical patent/DK0832200T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/644—Coagulation factor IXa (3.4.21.22)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21022—Coagulation factor IXa (3.4.21.22)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Compounds Of Unknown Constitution (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
DK96915892T 1995-06-07 1996-05-21 Nye metoder til rensning af faktor IX DK0832200T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/472,823 US5714583A (en) 1995-06-07 1995-06-07 Factor IX purification methods
PCT/US1996/007305 WO1996040883A1 (en) 1995-06-07 1996-05-21 Novel factor ix purification methods

Publications (1)

Publication Number Publication Date
DK0832200T3 true DK0832200T3 (da) 2008-07-21

Family

ID=23877079

Family Applications (2)

Application Number Title Priority Date Filing Date
DK05012614.3T DK1571208T3 (da) 1995-06-07 1996-05-21 Fremgangsmåde til ny faktor IX-rensning
DK96915892T DK0832200T3 (da) 1995-06-07 1996-05-21 Nye metoder til rensning af faktor IX

Family Applications Before (1)

Application Number Title Priority Date Filing Date
DK05012614.3T DK1571208T3 (da) 1995-06-07 1996-05-21 Fremgangsmåde til ny faktor IX-rensning

Country Status (11)

Country Link
US (2) US5714583A (de)
EP (2) EP1571208B1 (de)
JP (1) JP3676817B2 (de)
AT (2) ATE393217T1 (de)
AU (1) AU5754396A (de)
CA (1) CA2220501C (de)
DE (2) DE69637509T2 (de)
DK (2) DK1571208T3 (de)
ES (2) ES2306450T3 (de)
PT (2) PT832200E (de)
WO (1) WO1996040883A1 (de)

Families Citing this family (38)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6150328A (en) 1986-07-01 2000-11-21 Genetics Institute, Inc. BMP products
US6291206B1 (en) 1993-09-17 2001-09-18 Genetics Institute, Inc. BMP receptor proteins
DE69434651T2 (de) 1993-12-07 2007-03-08 Genetics Institute, Inc., Cambridge Bmp-12, bmp-13 und diese enthaltende sehne-induzierende zusammensetzungen
US5714583A (en) * 1995-06-07 1998-02-03 Genetics Institute, Inc. Factor IX purification methods
US20030036629A1 (en) * 1997-12-12 2003-02-20 Barry Foster Novel tgf-beta protein purification methods
PT1308456E (pt) 1998-05-06 2007-12-03 Genentech Inc Purificação de anticorpos por cromatografia de permuta iónica
JP2002526455A (ja) 1998-09-21 2002-08-20 ジェネティックス インスティテュート インク． 治療用たんぱく質に対する免疫応答を下方変調する方法
US6727224B1 (en) 1999-02-01 2004-04-27 Genetics Institute, Llc. Methods and compositions for healing and repair of articular cartilage
PT1223990E (pt) 1999-10-15 2004-12-31 Fidia Advanced Biopolymers Srl Formulacoes de acido hialuronico para administracao de proteinas osteogenicas
WO2002039951A2 (en) * 2000-11-15 2002-05-23 Globe Immune, Inc. Yeast-dentritic cell vaccines and uses thereof
EP1399023B1 (de) 2001-06-01 2008-04-30 Wyeth Zusammensetzungen für die systemische verabreichung von sequenzen, die für knochenmorphogenese-proteinen kodieren
TWI267378B (en) 2001-06-08 2006-12-01 Wyeth Corp Calcium phosphate delivery vehicles for osteoinductive proteins
KR100451308B1 (ko) * 2001-12-28 2004-10-06 선바이오(주) 단백질 의약품 정제 방법에서의 바이러스 제거 방법
JP2006517415A (ja) 2003-01-09 2006-07-27 ジェネンテック・インコーポレーテッド ポリペプチドの精製
MXPA05010846A (es) * 2003-04-09 2006-03-30 Nektar Therapeutics Tratamiento de hemofilia mediante inhalacion de factores de coagulacion.
KR101146160B1 (ko) * 2004-06-30 2012-07-16 넥타르 테라퓨틱스 중합체­인자 ｉｘ 부분의 접합체
EP1969127B8 (de) 2005-12-21 2017-12-13 Aptevo BioTherapeutics LLC Verfahren zur herstellung biologisch wirksamer vitamin-k-abhängiger proteine mit rekombinanten verfahren
EP2004683B1 (de) * 2006-03-24 2016-05-11 Biogen Hemophilia Inc. Pc5 als faktor-ix-propeptid-verarbeitendes enzym
WO2008104372A1 (en) * 2007-02-28 2008-09-04 Baxter International Inc. Method for the purification of recombinant blood coagulation factor ix enriched in sulfated and/or phosphorylated molecules
AU2008245524A1 (en) * 2007-04-26 2008-11-06 Cnj Holdings, Inc. Recombinant vitamin K dependent proteins with high sialic acid content and methods of preparing same
RU2353386C1 (ru) * 2007-10-11 2009-04-27 Общество с ограниченной ответственностью "БиоГениус" Способ получения концентрата ix фактора свертывания крови человека
CN101889025B (zh) 2007-10-30 2013-11-13 健泰科生物技术公司 通过阳离子交换层析进行的抗体纯化
US20110166332A1 (en) * 2008-09-12 2011-07-07 Ge Healthcare Bio-Sciences Ab Enhanced antibody aggregate removal with capto adhere in the presence of protein-excluded zwitterions
FR2946348B1 (fr) 2009-06-05 2011-08-05 Lab Francais Du Fractionnement Procede de preparation d'une composition de complexe prothrombique a haut degre de purete
DK2451963T3 (da) 2009-07-10 2014-08-04 Csl Ltd Fremgangsmåde til forøgelse afvitamin-k-afhængige proteiners exprimerings-ydelse
WO2011053738A1 (en) 2009-10-30 2011-05-05 Inspiration Biopharmaceuticals, Inc. Method of producing recombinant vitamin k dependent proteins
WO2011064247A1 (en) 2009-11-24 2011-06-03 Novo Nordisk Health Care Ag Method of purifying pegylated proteins
EP2553095B1 (de) * 2010-03-30 2016-10-12 Octapharma AG Verfahren zur Reinigung von Vitamin-K-abhängigen Proteinen wie dem Blutgerinnungsfaktor IX
DK2552948T3 (en) 2010-03-30 2016-10-17 Octapharma Ag A process for the purification of the protein growth factor G-CSF
MX336728B (es) * 2010-04-29 2016-01-27 Baxter Int Metodo de purificacion para proteinas de union de cation divalente en resina de intercambio anionico.
CA2821711C (en) 2010-12-15 2017-10-10 Baxter International Inc. Eluate collection using conductivity gradient
BRPI1105317A2 (pt) 2011-01-24 2013-04-30 Fundacco Hemoct De Ribeirco Preto produÇço estÁvel e em larga escala de fviii humano em linhagem celular humana sk-hep-1
CN103814137A (zh) * 2011-09-06 2014-05-21 米迪缪尼有限公司 处理凝血因子的方法
AU2012322948B2 (en) 2011-10-14 2014-11-06 Takeda Pharmaceutical Company Limited Protein purification by anion exchange chromatography
JP6117219B2 (ja) 2011-10-14 2017-04-19 バクスアルタ ゲーエムベーハー 陰イオン交換クロマトグラフィによるタンパク質の精製方法
WO2014140289A1 (en) 2013-03-15 2014-09-18 Baxter International Inc. Purification method for vitamin k dependent proteins by anion exchange chromatography
US9663553B2 (en) 2014-01-29 2017-05-30 Hemarus Therapeutics Limited Integrated process for the production of therapeutics (human albumin, immunoglobulins, clotting factor VIII and clotting factor IX) from human plasma
CN114134109B (zh) * 2021-12-10 2023-01-24 广州远想生物科技股份有限公司 Egf间充质干细胞外泌体的纯化方法

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3101752A1 (de) * 1981-01-21 1982-08-26 Behringwerke Ag, 3550 Marburg "verfahren zur reinigung der blutgerinnungsfaktoren ii, vii, ix und/oder x und danach hergestellte praeparationen"
US4447416A (en) * 1982-04-28 1984-05-08 American National Red Cross Plasma protein concentrates of reduced thrombogenicity and their use in clinical replacement therapy
US5055557A (en) * 1983-03-04 1991-10-08 Scripps Clinic & Research Foundation Ultrapurification of factor IX and other vitamin K-dependent proteins
JPS61500226A (ja) * 1983-10-28 1986-02-06 ニュ−・イングランド・メディカル・センタ−・ホスピタルズ・インコ−ポレ−テッド コンホメ−ション特異性抗体を用いる血液凝固タンパク質の精製および単離法
US4721572A (en) * 1985-07-12 1988-01-26 Miles Laboratories, Inc. Purfication of blood clotting factors and other blood proteins on non-carbohydrate sulfated matrices
US4786726A (en) * 1986-01-06 1988-11-22 Blood Systems, Inc. Factor IX therapeutic blood product, means and methods of preparing same
US4725673A (en) * 1986-08-29 1988-02-16 Alpha Therapeutic Corporation Plasma fraction purification using silica resin bound to a ligand
US4831118A (en) * 1987-08-07 1989-05-16 Scripps Clinic And Research Foundation Flouroplastic immunoaffinity columns for purification of blood proteins
DE3877529T2 (de) * 1987-10-23 1996-11-07 Centre Regional De Transfusion Herstellung von hoch-gereingtem menschlichen Faktor IX sowie anderem Plasmaproteinkonzentrat und dessen therapeutische Verwendung.
WO1989005652A1 (en) * 1987-12-21 1989-06-29 American National Red Cross Pure factor ix product
GB8729822D0 (en) * 1987-12-22 1988-02-03 Central Blood Lab Authority Chemical process
CA1330302C (en) * 1988-01-18 1994-06-21 Miroslav Rybak Concentrates of coagulation factors ii, vii, ix and x, method of their preparation and use
DE3826792C1 (de) * 1988-08-06 1989-07-06 Biotest Pharma Gmbh, 6072 Dreieich, De
US4981952A (en) * 1988-10-04 1991-01-01 Eli Lilly And Company Method for the purification of vitamin K-dependent proteins
DE3914869C1 (de) * 1989-05-05 1990-08-09 Biotest Pharma Gmbh, 6072 Dreieich, De
AT402261B (de) * 1991-01-25 1997-03-25 Immuno Ag Komplex enthaltend den gerinnungsfaktor ix
IT1262899B (it) * 1992-03-27 1996-07-22 Sclavo Spa Processo per l'isolamento di fattore ix, fattore x e fattore ii altamente purificati dal complesso protrombinico o dal plasma umano
US5714583A (en) * 1995-06-07 1998-02-03 Genetics Institute, Inc. Factor IX purification methods

Also Published As

Publication number Publication date
ES2306450T3 (es) 2008-11-01
EP0832200B1 (de) 2008-04-23
CA2220501A1 (en) 1996-12-19
DE69638291D1 (de) 2010-12-30
JP3676817B2 (ja) 2005-07-27
DK1571208T3 (da) 2011-01-24
PT832200E (pt) 2008-07-17
DE69637509D1 (de) 2008-06-05
ES2353798T3 (es) 2011-03-07
AU5754396A (en) 1996-12-30
ATE488582T1 (de) 2010-12-15
EP0832200A1 (de) 1998-04-01
DE69637509T2 (de) 2009-05-28
JPH11506603A (ja) 1999-06-15
EP1571208A1 (de) 2005-09-07
EP1571208B1 (de) 2010-11-17
US5714583A (en) 1998-02-03
CA2220501C (en) 2014-01-07
US6627737B1 (en) 2003-09-30
PT1571208E (pt) 2011-01-04
ATE393217T1 (de) 2008-05-15
WO1996040883A1 (en) 1996-12-19

Similar Documents

Publication Publication Date Title
DK0832200T3 (da) Nye metoder til rensning af faktor IX
ATE106897T1 (de) Neue fusionsproteine und deren reinigung.
CA2132533A1 (en) Protein purification
DE69602638D1 (de) Naphthylverbindungen, Zwischenprodukte, Zusammensetzungen und Methoden
SE9404250D0 (sv) Affinity separation method
NO994998D0 (no) Fusjonsproteiner
IL109925A0 (en) Heteroatom-bearing compounds metal complexes thereof, and pharmaceutical kits containing the same
ITTO940751A0 (it) Procedimento per la produzione di carburo di ferro.
DE69023413D1 (de) Reinigung des Faktor-VIII-Komplexes unter Anwendung von Heparinaffinitätschromatographie.
CA2220447A1 (en) Novel fusion protein recovery and purification methods
DK0937055T3 (da) Fremgangsmåde til fremstilling af d,I-alfa-tocopherol
ATE167670T1 (de) N-alkylthiopolyaminderivate als radioprotektive mittel
NO961604D0 (no) Ketogruppe-introduserende enzym, DNA som koder for samme, samt fremgangsmåte for fremstilling av ketokarotenoid
ATE88718T1 (de) Verfahren zur reinigung des hepatitis-oberflaechen-antigens und das so erhaltene produkt.
DK0770077T3 (da) Fremgangsmåde til fremstilling af 2-(1-azabicyclo[2.2.2]oct-3-yl)-2,4,5,6-tetrahydro-1h-benz[de]isoquinolin-1-on og mellemprodukter
DK0407212T3 (da) Enantioselektiv syntese af antifolater
EP0577253A3 (de) Anreicherung von Quinuklidinolenantiomeren.
DK0815092T3 (da) Fremgangsmåde til fremstilling af 1,2-benzisothiazolin-3-oner
DK0683168T3 (da) Fremgangsmåde til fremstilling af 2-methylspiro(1,3-oxathiolan-5,3')quinuclidin
DK0735043T3 (da) Fremgangsmåde til fremstilling af 2-substitueret 3,3-difluorfuran
DK0820468T3 (da) Fremgangsmåde til rensning af glycoproteiner, såsom erythropoietin
FR2703075B1 (fr) Procede de preparation de diaphragme microporeux.
ES2134518T3 (es) Ligante de expansion.
DK0783491T3 (da) Fremgangsmåde til fremstilling af 3,3,5-trichlorglutarimid
SE9302856D0 (sv) Method and means for producing a plasmapeotein-binding protein