ATE393217T1 - Verfahren zur reinigung von faktor-ix - Google Patents

Verfahren zur reinigung von faktor-ix

Info

Publication number
ATE393217T1
ATE393217T1 AT96915892T AT96915892T ATE393217T1 AT E393217 T1 ATE393217 T1 AT E393217T1 AT 96915892 T AT96915892 T AT 96915892T AT 96915892 T AT96915892 T AT 96915892T AT E393217 T1 ATE393217 T1 AT E393217T1
Authority
AT
Austria
Prior art keywords
purification
factor
hydroxyapatite
resins
anion exchange
Prior art date
Application number
AT96915892T
Other languages
English (en)
Inventor
W Foster
Robert Costigan
Duane Bonam
Mary Switzer
Rochelle Walsh
Original Assignee
Genetics Inst Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genetics Inst Llc filed Critical Genetics Inst Llc
Application granted granted Critical
Publication of ATE393217T1 publication Critical patent/ATE393217T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/644—Coagulation factor IXa (3.4.21.22)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21022—Coagulation factor IXa (3.4.21.22)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Compounds Of Unknown Constitution (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
AT96915892T 1995-06-07 1996-05-21 Verfahren zur reinigung von faktor-ix ATE393217T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/472,823 US5714583A (en) 1995-06-07 1995-06-07 Factor IX purification methods

Publications (1)

Publication Number Publication Date
ATE393217T1 true ATE393217T1 (de) 2008-05-15

Family

ID=23877079

Family Applications (2)

Application Number Title Priority Date Filing Date
AT05012614T ATE488582T1 (de) 1995-06-07 1996-05-21 Verfahren zur reinigung von faktor ix
AT96915892T ATE393217T1 (de) 1995-06-07 1996-05-21 Verfahren zur reinigung von faktor-ix

Family Applications Before (1)

Application Number Title Priority Date Filing Date
AT05012614T ATE488582T1 (de) 1995-06-07 1996-05-21 Verfahren zur reinigung von faktor ix

Country Status (11)

Country Link
US (2) US5714583A (de)
EP (2) EP0832200B1 (de)
JP (1) JP3676817B2 (de)
AT (2) ATE488582T1 (de)
AU (1) AU5754396A (de)
CA (1) CA2220501C (de)
DE (2) DE69637509T2 (de)
DK (2) DK1571208T3 (de)
ES (2) ES2353798T3 (de)
PT (2) PT1571208E (de)
WO (1) WO1996040883A1 (de)

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US6150328A (en) 1986-07-01 2000-11-21 Genetics Institute, Inc. BMP products
US6291206B1 (en) 1993-09-17 2001-09-18 Genetics Institute, Inc. BMP receptor proteins
EP0733109B9 (de) 1993-12-07 2006-07-05 Genetics Institute, LLC Bmp-12, bmp-13 und diese enthaltende sehne-induzierende zusammensetzungen
US5714583A (en) * 1995-06-07 1998-02-03 Genetics Institute, Inc. Factor IX purification methods
US20030036629A1 (en) * 1997-12-12 2003-02-20 Barry Foster Novel tgf-beta protein purification methods
ATE370961T1 (de) 1998-05-06 2007-09-15 Genentech Inc Reinigung von antikörpern durch ionenaustauschchromatographie
HK1039059A1 (zh) 1998-09-21 2002-04-12 Genetics Institute, Llc 负调节对治疗蛋白的免疫应答的方法
US6727224B1 (en) 1999-02-01 2004-04-27 Genetics Institute, Llc. Methods and compositions for healing and repair of articular cartilage
EP1223990B1 (de) 1999-10-15 2004-07-28 Genetics Institute, LLC Hyaluronsäure enthaltende zusammensetzungen zur abgabe osteogener proteine
AU2002225681A1 (en) * 2000-11-15 2002-05-27 Globe Immune, Inc. Yeast-dentritic cell vaccines and uses thereof
US7226587B2 (en) 2001-06-01 2007-06-05 Wyeth Compositions and methods for systemic administration of sequences encoding bone morphogenetic proteins
TWI267378B (en) 2001-06-08 2006-12-01 Wyeth Corp Calcium phosphate delivery vehicles for osteoinductive proteins
KR100451308B1 (ko) * 2001-12-28 2004-10-06 선바이오(주) 단백질 의약품 정제 방법에서의 바이러스 제거 방법
NZ540895A (en) 2003-01-09 2007-03-30 Genentech Inc Purification of polypeptides with ethacridine lactate
WO2004091487A2 (en) * 2003-04-09 2004-10-28 Wyeth Hemophilia treatment by inhalation of coagulation factors
JP2008505119A (ja) * 2004-06-30 2008-02-21 ネクター セラピューティクス アラバマ，コーポレイション 高分子−第ｉｘ因子部分の抱合体
ES2503365T5 (es) 2005-12-21 2017-10-31 Cnj Holdings, Inc Método para producir proteínas dependientes de vitamina K biológicamente activas por métodos recombinantes
TWI428448B (zh) * 2006-03-24 2014-03-01 Syntonix Pharmaceuticals Inc 作為第九因子（ｆａｃｔｏｒ ｉｘ）原肽處理酶之ｐｃ５
US8399632B2 (en) * 2007-02-28 2013-03-19 Baxter International Inc. Method for the purification of recombinant blood coagulation factor IX enriched in sulfated and/or phosphorylated molecules
AU2008245524A1 (en) * 2007-04-26 2008-11-06 Cnj Holdings, Inc. Recombinant vitamin K dependent proteins with high sialic acid content and methods of preparing same
RU2353386C1 (ru) * 2007-10-11 2009-04-27 Общество с ограниченной ответственностью "БиоГениус" Способ получения концентрата ix фактора свертывания крови человека
HUE024877T2 (en) 2007-10-30 2016-02-29 Genentech Inc Antibody purification by cation exchange chromatography
WO2010030222A1 (en) * 2008-09-12 2010-03-18 Ge Healthcare Bio-Sciences Ab Enhanced protein aggregate removal with multimodal anion exchangers in the presence of protein-excluded zwitterions
FR2946348B1 (fr) 2009-06-05 2011-08-05 Lab Francais Du Fractionnement Procede de preparation d'une composition de complexe prothrombique a haut degre de purete
CN102471794B (zh) 2009-07-10 2014-10-29 Csl有限公司 提高维生素k依赖性蛋白质的表达产量的方法
WO2011053738A1 (en) 2009-10-30 2011-05-05 Inspiration Biopharmaceuticals, Inc. Method of producing recombinant vitamin k dependent proteins
CN107383158A (zh) 2009-11-24 2017-11-24 诺沃—诺迪斯克保健股份有限公司 纯化聚乙二醇化蛋白质的方法
CN102858971B (zh) * 2010-03-30 2016-06-01 奥克塔法马股份有限公司 纯化维生素k依赖性蛋白如凝血因子ix的方法
EP2552948B1 (de) 2010-03-30 2016-07-13 Octapharma AG Verfahren zur reinigung des wachstumsfaktorproteins g-csf
CN103025757A (zh) * 2010-04-29 2013-04-03 巴克斯特国际有限公司 二价阳离子结合蛋白在阴离子交换树脂上的纯化方法
EP2652491B1 (de) 2010-12-15 2017-11-29 Baxalta GmbH Eluatsammlung mit leitfähigkeitsgradient
BRPI1105317A2 (pt) 2011-01-24 2013-04-30 Fundacco Hemoct De Ribeirco Preto produÇço estÁvel e em larga escala de fviii humano em linhagem celular humana sk-hep-1
US9598687B2 (en) 2011-09-06 2017-03-21 Medimmune, Llc Methods for processing coagulation factors
PL2748179T3 (pl) * 2011-10-14 2022-04-11 Takeda Pharmaceutical Company Limited Oczyszczanie białka metodą anionowej chromatografii jonowymiennej
WO2013053888A1 (en) * 2011-10-14 2013-04-18 Baxter International Inc. Protein purification by anion exchange chromatography
TR201808584T4 (tr) 2013-03-15 2018-07-23 Baxalta GmbH Anyon değişimi kromotografisi aracılığıyla k vitaminine bağlı proteinler için saflaştırma yöntemi.
US9663553B2 (en) 2014-01-29 2017-05-30 Hemarus Therapeutics Limited Integrated process for the production of therapeutics (human albumin, immunoglobulins, clotting factor VIII and clotting factor IX) from human plasma
CN114134109B (zh) * 2021-12-10 2023-01-24 广州远想生物科技股份有限公司 Egf间充质干细胞外泌体的纯化方法

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3101752A1 (de) * 1981-01-21 1982-08-26 Behringwerke Ag, 3550 Marburg "verfahren zur reinigung der blutgerinnungsfaktoren ii, vii, ix und/oder x und danach hergestellte praeparationen"
US4447416A (en) * 1982-04-28 1984-05-08 American National Red Cross Plasma protein concentrates of reduced thrombogenicity and their use in clinical replacement therapy
US5055557A (en) * 1983-03-04 1991-10-08 Scripps Clinic & Research Foundation Ultrapurification of factor IX and other vitamin K-dependent proteins
EP0162078B1 (de) * 1983-10-28 1994-09-14 New England Medical Center Hospitals, Inc. VERFAHREN ZUR ISOLIERUNG EINES PROTEINS AUS EINER DIESES PROTEIN ENTHALTENDEN MISCHUNG UNTER VERWENDUNG EINES KONFORMATIONSSPEZIFISCHEN ANTIKöRPERS
US4721572A (en) * 1985-07-12 1988-01-26 Miles Laboratories, Inc. Purfication of blood clotting factors and other blood proteins on non-carbohydrate sulfated matrices
US4786726A (en) * 1986-01-06 1988-11-22 Blood Systems, Inc. Factor IX therapeutic blood product, means and methods of preparing same
US4725673A (en) * 1986-08-29 1988-02-16 Alpha Therapeutic Corporation Plasma fraction purification using silica resin bound to a ligand
US4831118A (en) * 1987-08-07 1989-05-16 Scripps Clinic And Research Foundation Flouroplastic immunoaffinity columns for purification of blood proteins
ES2045167T5 (es) * 1987-10-23 1996-07-01 Centre Regional De Transfusion Preparacion de concentrado de factor ix humano de elevada pureza y de otras proteinas plasmaticas.
WO1989005652A1 (en) * 1987-12-21 1989-06-29 American National Red Cross Pure factor ix product
GB8729822D0 (en) * 1987-12-22 1988-02-03 Central Blood Lab Authority Chemical process
US5118614A (en) * 1988-01-18 1992-06-02 Tessek Sdruzeni Praha Concentrates of coagulation factors ii, vii, ix and x, method of their preparation and use
DE3826792C1 (de) * 1988-08-06 1989-07-06 Biotest Pharma Gmbh, 6072 Dreieich, De
US4981952A (en) * 1988-10-04 1991-01-01 Eli Lilly And Company Method for the purification of vitamin K-dependent proteins
DE3914869C1 (de) * 1989-05-05 1990-08-09 Biotest Pharma Gmbh, 6072 Dreieich, De
AT402261B (de) * 1991-01-25 1997-03-25 Immuno Ag Komplex enthaltend den gerinnungsfaktor ix
IT1262899B (it) * 1992-03-27 1996-07-22 Sclavo Spa Processo per l'isolamento di fattore ix, fattore x e fattore ii altamente purificati dal complesso protrombinico o dal plasma umano
US5714583A (en) * 1995-06-07 1998-02-03 Genetics Institute, Inc. Factor IX purification methods

Also Published As

Publication number Publication date
DK0832200T3 (da) 2008-07-21
AU5754396A (en) 1996-12-30
CA2220501C (en) 2014-01-07
EP1571208B1 (de) 2010-11-17
DK1571208T3 (da) 2011-01-24
ES2353798T3 (es) 2011-03-07
WO1996040883A1 (en) 1996-12-19
DE69637509T2 (de) 2009-05-28
PT832200E (pt) 2008-07-17
US5714583A (en) 1998-02-03
JPH11506603A (ja) 1999-06-15
JP3676817B2 (ja) 2005-07-27
PT1571208E (pt) 2011-01-04
ES2306450T3 (es) 2008-11-01
EP1571208A1 (de) 2005-09-07
EP0832200A1 (de) 1998-04-01
CA2220501A1 (en) 1996-12-19
US6627737B1 (en) 2003-09-30
DE69637509D1 (de) 2008-06-05
DE69638291D1 (de) 2010-12-30
EP0832200B1 (de) 2008-04-23
ATE488582T1 (de) 2010-12-15

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