DK2496692T3 - Polypeptider med xylanaseaktivitet og polynukleotider, der koder for dem - Google Patents

Polypeptider med xylanaseaktivitet og polynukleotider, der koder for dem Download PDF

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DK2496692T3
DK2496692T3 DK10776907.7T DK10776907T DK2496692T3 DK 2496692 T3 DK2496692 T3 DK 2496692T3 DK 10776907 T DK10776907 T DK 10776907T DK 2496692 T3 DK2496692 T3 DK 2496692T3
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polypeptide
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ala
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ser
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DK10776907.7T
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Elena Vlasenko
Brett Mcbrayer
Dominique Skovlund
Sara Landvik
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Novozymes Inc
Novozymes As
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/248—Xylanases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2462—Lysozyme (3.2.1.17)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01008—Endo-1,4-beta-xylanase (3.2.1.8)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

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  • Chemical Kinetics & Catalysis (AREA)
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  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Claims (20)

1. Isoleret polypeptid med xylanaseaktivitet, som er valgt fra gruppen bestående af: (a) et polypeptid, som omfatter en aminosyresekvens, der udviser mindst 65% identitet med det modne polypeptid ifølge SEQ ID NO: 2; (b) et polypeptid, som kodes for af et polynukleotid, der omfatter en nukleotidsekvens, som udviser mindst 65% identitet med det modne polypeptids kodningssekvens ifølge SEQ ID NO: 1; og (c) en variant, som omfatter en substitution, deletion og/eller insertion af 1-5 aminosyrer i det modne polypeptid ifølge SEQ ID NO: 2.
2. Polypeptid ifølge krav 1, som omfatter eller består af aminosyresekvensen ifølge SEQ ID NO: 2; eller et fragment deraf med xylanaseaktivitet.
3. Polypeptid ifølge krav 1, hvilket polypeptid kodes for af polynukleotidet indeholdt i plasmid pTF12Xyl170, hvilket plasmid er indeholdt i E. coli NRRL B-50309.
4. Isoleret polynukleotid, som omfatter en nukleotidsekvens, der koder for polypeptidet ifølge et hvilket som helst af kravene 1-3.
5. Fremgangsmåde til fremstilling af polypeptidet ifølge et hvilket som helst af kravene 1-3, hvilken fremgangsmåde omfatter: (a) dyrkning af en celle, som i sin vildtypeform fremstiller polypeptidet, under betingelser, der er befordrende for fremstilling af polypeptidet; og (b) indvinding af polypeptidet.
6. Fremgangsmåde til fremstilling af polypeptidet ifølge et hvilket som helst af kravene 1-3, hvilken fremgangsmåde omfatter: (a) dyrkning af en værtscelle, som omfatter en nukleinsyrekonstruktion, hvilken nukleinsyrekonstruktion omfatter en nukleotidsekvens, der koder for polypeptidet, under betingelser, som er befordrende for fremstilling af polypeptidet; og (b) indvinding af polypeptidet.
7. Fremgangsmåde til fremstilling af en mutant af en stamcelle, hvilken fremgangsmåde omfatter introduktion af forstyrrelse i eller deletion af et polynukleotid, der koder for polypeptidet, eller en del deraf, ifølge et hvilket som helst af kravene 1-3, hvilket resulterer i, at mutanten fremstiller mindre af polypeptidet end stamcellen.
8. Fremgangsmåde til fremstilling af polypeptidet ifølge et hvilket som helst af kravene 1-3, hvilken fremgangsmåde omfatter: (a) dyrkning af en transgen plante eller en plantecelle, som omfatter et polynukleotid, hvilket polynukleotid koder for polypeptidet, under betingelser, der er befordrende for fremstilling af polypeptidet; og (b) indvinding af polypeptidet.
9. Transgen plante, plantedel eller plantecelle, som indeholder polypeptidet ifølge et hvilket som helst af kravene 1-3.
10. Dobbeltstrenget hæmmende RNA (dsRNA)-molekyle, som omfatter en undersekvens af polynukleotidet ifølge krav 4, hvor dsRNA'et eventuelt er et siRNA- eller miRNA-molekyle.
11. Fremgangsmåde til hæmning af ekspressionen af et polypeptid med xylanaseaktivitet i en celle, hvilken fremgangsmåde omfatter administration til cellen eller ekspression i cellen af det dobbeltstrengede hæmmende RNA-molekyle ifølge krav 10.
12. Isoleret polynukleotid, som koder for et signalpeptid, der omfatter eller består af aminosyrerne 1 til 19 ifølge SEQ ID NO: 2.
13. Fremgangsmåde til fremstilling af et protein, hvilken fremgangsmåde omfatter: (a) dyrkning af en rekombinant værtscelle, som omfatter et gen, der koder for et protein, som er operativt forbundet til polynukleotidet ifølge krav 12, hvor genet er fremmed for polynukleotidet, under betingelser, der er befordrende for fremstilling af proteinet; og (b) indvinding af proteinet.
14. Fremgangsmåde til nedbrydning eller omdannelse af et celluloseholdigt materiale eller xylanholdigt materiale, hvilken fremgangsmåde omfatter: behandling af det celluloseholdige materiale eller xylanholdige materiale med en enzymsammensætning i nærværelse af polypeptidet ifølge et hvilket som helst af kravene 1-3.
15. Fremgangsmåde ifølge krav 14, hvilken fremgangsmåde endvidere omfatter indvinding af det nedbrudte celluloseholdige materiale eller xylanholdige materiale.
16. Fremgangsmåde til fremstilling af et fermenteringsprodukt, hvilken fremgangsmåde omfatter: (a) forsukring af et celluloseholdigt materiale eller xylanholdigt materiale med en enzymsammensætning i nærværelse af polypeptidet ifølge et hvilket som helst af kravene 1-3; (b) fermentering af det forsukrede celluloseholdige materiale eller xylanholdige materiale med en eller flere (adskillige) fermenterende mikroorganismer for at fremstille fermenteringsproduktet; og (c) indvinding af fermenteringsproduktet fra fermenteringen.
17. Fremgangsmåde til fermentering af et celluloseholdigt materiale eller xylanholdigt materiale, hvilken fremgangsmåde omfatter: fermentering af det celluloseholdige materiale eller xylanholdige materiale med en eller flere (adskillige) fermenterende mikroorganismer, hvor det celluloseholdige materiale eller xylanholdige materiale er forsukret med en enzymsammensætning i nærværelse af et polypeptid ifølge et hvilket som helst af kravene 1-3.
18. Fremgangsmåde ifølge krav 17, hvor fermenteringen af det celluloseholdige materiale eller xylanholdige materiale frembringer et fermenteringsprodukt.
19. Fremgangsmåde ifølge krav 18, hvilken fremgangsmåde endvidere omfatter indvinding af fermenteringsproduktet fra fermenteringen.
20. Fremgangsmåde ifølge krav 18 eller 19, hvor fermenteringsproduktet er en alkohol, en organisk syre, en keton, en aminosyre eller en gas.
DK10776907.7T 2009-11-06 2010-11-05 Polypeptider med xylanaseaktivitet og polynukleotider, der koder for dem DK2496692T3 (da)

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US25900609P 2009-11-06 2009-11-06
PCT/US2010/055640 WO2011057083A1 (en) 2009-11-06 2010-11-05 Polypeptides having xylanase activity and polynucleotides encoding same

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US20170145395A1 (en) 2017-05-25
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US20120278952A1 (en) 2012-11-01
EP2496692A1 (en) 2012-09-12
CN102639697A (zh) 2012-08-15
CN104694517B (zh) 2019-06-28
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