DK82293A - Normalt sporulerende bacillus uden evne til at udskille subtilisin eller neutral protease, kulturer deraf og fremgangsmaade til fremstilling af heterologe proteiner dermed - Google Patents

Normalt sporulerende bacillus uden evne til at udskille subtilisin eller neutral protease, kulturer deraf og fremgangsmaade til fremstilling af heterologe proteiner dermed

Info

Publication number
DK82293A
DK82293A DK082293A DK82293A DK82293A DK 82293 A DK82293 A DK 82293A DK 082293 A DK082293 A DK 082293A DK 82293 A DK82293 A DK 82293A DK 82293 A DK82293 A DK 82293A
Authority
DK
Denmark
Prior art keywords
neutral protease
subtilisin
cultures
procedures
ability
Prior art date
Application number
DK082293A
Other languages
English (en)
Other versions
DK175768B1 (da
DK82293D0 (da
Inventor
Richard Ray Bott
David Aaron Estell
Eugenio Ferrari
Dennis James Henner
James Allen Wells
Original Assignee
Genentech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=27560079&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DK82293(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority claimed from US06/614,612 external-priority patent/US4760025A/en
Application filed by Genentech Inc filed Critical Genentech Inc
Publication of DK82293D0 publication Critical patent/DK82293D0/da
Publication of DK82293A publication Critical patent/DK82293A/da
Application granted granted Critical
Publication of DK175768B1 publication Critical patent/DK175768B1/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07—Bacillus

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Detergent Compositions (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DK199300822A 1983-06-24 1993-07-08 Normalt sporulerence Bacillus stamme uden evne til at udskille subtilisin eller neutral protease og fremgangsmåde til fremstilling af proteiner dermed DK175768B1 (da)

Applications Claiming Priority (12)

Application Number Priority Date Filing Date Title
US50741983A 1983-06-24 1983-06-24
US50741983 1983-06-24
US61461684A 1984-05-29 1984-05-29
US61461584A 1984-05-29 1984-05-29
US61449184A 1984-05-29 1984-05-29
US61461784A 1984-05-29 1984-05-29
US61461584 1984-05-29
US61461684 1984-05-29
US61461784 1984-05-29
US61449184 1984-05-29
US61461284 1984-05-29
US06/614,612 US4760025A (en) 1984-05-29 1984-05-29 Modified enzymes and methods for making same

Publications (3)

Publication Number Publication Date
DK82293D0 DK82293D0 (da) 1993-07-08
DK82293A true DK82293A (da) 1993-07-08
DK175768B1 DK175768B1 (da) 2005-02-14

Family

ID=27560079

Family Applications (3)

Application Number Title Priority Date Filing Date
DK198403059A DK175278B1 (da) 1983-06-24 1984-06-22 Fremgangsmåde til frembringelse af mutation i DNA-sekvens, der koder for et Bacillus-subtilisin-enzym eller dets præ- eller præproensym samt fremgangsmåde til fremstilling af et mutant-subtilisin-enzym med en önsket
DK199300822A DK175768B1 (da) 1983-06-24 1993-07-08 Normalt sporulerence Bacillus stamme uden evne til at udskille subtilisin eller neutral protease og fremgangsmåde til fremstilling af proteiner dermed
DK082393A DK82393A (da) 1983-06-24 1993-07-08 Fremgangsmaade til frembringelse af mutationer ved hvert enkelt sted i kodeomraadet for et protein og til udtrykkelse af de dannede mutant-dna-dele under opnaaelse af mutantproteiner.

Family Applications Before (1)

Application Number Title Priority Date Filing Date
DK198403059A DK175278B1 (da) 1983-06-24 1984-06-22 Fremgangsmåde til frembringelse af mutation i DNA-sekvens, der koder for et Bacillus-subtilisin-enzym eller dets præ- eller præproensym samt fremgangsmåde til fremstilling af et mutant-subtilisin-enzym med en önsket

Family Applications After (1)

Application Number Title Priority Date Filing Date
DK082393A DK82393A (da) 1983-06-24 1993-07-08 Fremgangsmaade til frembringelse af mutationer ved hvert enkelt sted i kodeomraadet for et protein og til udtrykkelse af de dannede mutant-dna-dele under opnaaelse af mutantproteiner.

Country Status (8)

Country Link
EP (4) EP0247647B1 (da)
JP (3) JP2594533B2 (da)
AT (2) ATE60356T1 (da)
DE (3) DE3486139T2 (da)
DK (3) DK175278B1 (da)
HK (3) HK75393A (da)
IE (1) IE81141B1 (da)
NZ (1) NZ208612A (da)

Families Citing this family (224)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ208612A (en) 1983-06-24 1991-09-25 Genentech Inc Method of producing "procaryotic carbonyl hydrolases" containing predetermined, site specific mutations
CA1311429C (en) * 1983-07-06 1992-12-15 Vasantha Nagarajan Vector for polypeptides in bacilli
JPH0659224B2 (ja) * 1983-09-12 1994-08-10 工業技術院長 Ｄνａ塩基配列及びそのｄνａ塩基配列を含む組換え体プラスミドの創製法
EP0462632A1 (en) * 1983-10-28 1991-12-27 Genetics Institute, Inc. Method for producing a desired human protein by recombinant DNA techniques
JPH07106156B2 (ja) * 1983-10-28 1995-11-15 ジェネティックス、インスティチュ−ト ファクタ−▲ｖｉｉｉ▼および関連生産物の製造
EP0149241B1 (en) * 1983-12-28 1991-03-27 Agency Of Industrial Science And Technology Dna base sequence containing regions involved in the production and secretion of a protein, recombinant dna including the whole or a part of the dna base sequence, and method of producing proteins by use of the recombinant dna
US5411873A (en) * 1984-05-29 1995-05-02 Genencor, Inc. Process for producing heterologous polypeptides
US5972682A (en) * 1984-05-29 1999-10-26 Genencor International, Inc. Enzymatically active modified subtilisins
US5185258A (en) 1984-05-29 1993-02-09 Genencor International, Inc. Subtilisin mutants
US4801537A (en) * 1984-06-08 1989-01-31 Genex Corporation Vector for expression of polypeptides in bacilli
US5840522A (en) * 1984-09-20 1998-11-24 Chiron Corporation Recombinant lectins
US4828994A (en) * 1984-09-21 1989-05-09 Genex Corporation Bacillus strains with reduced extracellular protease levels
US4752585A (en) * 1985-12-17 1988-06-21 Cetus Corporation Oxidation-resistant muteins
CA1340265C (en) * 1985-01-18 1998-12-15 Kirston E. Koths Oxidation resistant muteins
US6084073A (en) * 1985-03-25 2000-07-04 Chiron Corporation Recombinant ricin toxin
EP0316018A3 (en) * 1985-03-29 1989-07-26 Cetus Oncology Corporation Modification of dna sequences
DE3685768T2 (de) * 1985-04-15 1993-01-21 Procter & Gamble Fluessige reinigungsmittel mit einer anionischen oberflaechenaktiven verbindung, einem verstaerker und einem proteolytischen enzym.
ATE77649T1 (de) * 1985-04-15 1992-07-15 Procter & Gamble Fluessige reinigungsmittel mit einer oberflaechenaktiven verbindung, einem proteolytischen enzym und borsaeure.
IT1186750B (it) * 1985-07-10 1987-12-16 Eniricerche Spa Vettore di clonaggio,molecole di dna ricombinante,ceppi di bacillus subtilis trasformati con dette molecole e metodi per l'espressione di geni eterologhi e produzione e secrezione di proteine codificate da detti geni
DE3527913A1 (de) * 1985-08-03 1987-02-12 Henkel Kgaa Alkalische protease, verfahren zur herstellung von hybridvektoren und genetisch transformierte mikroorganismen
EP0254735B2 (en) * 1986-01-15 1998-06-17 Amgen Inc. METHOD FOR PRODUCTION OF THERMALLY STABLE AND pH STABLE SUBTILISIN ANALOGS
US5013657A (en) * 1988-04-12 1991-05-07 Bryan Philip N Subtilisin mutations
US4908773A (en) * 1987-04-06 1990-03-13 Genex Corporation Computer designed stabilized proteins and method for producing same
IE65767B1 (en) * 1986-04-30 1995-11-15 Genencor Int Non-human carbonyl hydrolase mutants DNA sequences and vectors encoding same and hosts transformed with said vectors
DE3781017T2 (de) * 1986-05-06 1993-03-18 Univ Leland Stanford Junior Cytotoxische zusammensetzung aus "killer"-zellen.
US5364554A (en) * 1986-06-09 1994-11-15 The Clorox Company Proteolytic perhydrolysis system and method of use for bleaching
EP0260350B1 (en) * 1986-09-05 1992-02-12 Cetus Oncology Corporation Oxidation-resistant interferon-beta muteins and their production; formulations containing such muteins
NZ221627A (en) * 1986-09-09 1993-04-28 Genencor Inc Preparation of enzymes, modifications, catalytic triads to alter ratios or transesterification/hydrolysis ratios
WO1988006623A1 (en) * 1987-02-27 1988-09-07 Gist-Brocades N.V. Stable gene amplification in chromosomal dna of prokaryotic microorganisms
WO1988006624A2 (en) * 1987-02-27 1988-09-07 Gist-Brocades N.V. Molecular cloning and expression of genes encoding proteolytic enzymes
ES2135386T3 (es) * 1987-02-27 1999-11-01 Genencor Int Transformacion de cepas de bacillus alcalofilas.
EP0285123A3 (en) * 1987-04-03 1989-02-01 Stabra AG A method for complete mutagenesis of nucleic acids
DE3856593D1 (de) * 1987-04-06 2007-10-04 Novozymes As Regelung von elektrostatischen interaktionen an metallionen-Bindungsstellen zur Stabilisierung von Proteinen
US4853871A (en) * 1987-04-06 1989-08-01 Genex Corporation Computer-based method for designing stablized proteins
US4914031A (en) * 1987-04-10 1990-04-03 Amgen, Inc. Subtilisin analogs
IT1228925B (it) * 1987-08-07 1991-07-10 Eniricerche Spa Procedimento per la preparazione dell'ormone della crescita umano
US5041378A (en) * 1987-08-11 1991-08-20 Cetus Corporation Procaryotic xylose isomerase muteins
US4939666A (en) * 1987-09-02 1990-07-03 Genex Corporation Incremental macromolecule construction methods
US4966846A (en) * 1987-10-01 1990-10-30 W. R. Grace & Co.-Conn. Molecular cloning and expression of a vibrio proteolyticus neutral protease gene
DK571587D0 (da) * 1987-11-02 1987-11-02 Novo Industri As Enzymatisk detergentsammensaetning
GB8727772D0 (en) * 1987-11-27 1987-12-31 Celltech Ltd Host cells
US4865983A (en) * 1987-12-04 1989-09-12 W. R. Grace & Co.-Conn. Cleaning compositions containing protease produced by vibrio and method of use
EP0319759B1 (en) * 1987-12-11 1993-01-07 Abbott Laboratories Method for mutagenesis by oligonucleotide-directed repair of a strand break
DK6488D0 (da) * 1988-01-07 1988-01-07 Novo Industri As Enzymer
US6287841B1 (en) 1988-02-11 2001-09-11 Genencor International, Inc. High alkaline serine protease
US5324653A (en) * 1988-02-11 1994-06-28 Gist-Brocades N.V. Recombinant genetic means for the production of serine protease muteins
CN1056187C (zh) 1988-02-11 2000-09-06 金克克国际有限公司 新的蛋白水解酶及其在洗涤剂中的应用
US5246849A (en) * 1988-04-12 1993-09-21 Enzon, Inc. Thermally stable serine proteases
US5118623A (en) * 1988-05-27 1992-06-02 Solvay Enzymes, Inc. Bleach stable enzymes
CA1333777C (en) 1988-07-01 1995-01-03 Randy M. Berka Aspartic proteinase deficient filamentous fungi
HU200796B (en) * 1988-07-14 1990-08-28 Richter Gedeon Vegyeszet Process for producing expression vectors ensuring insertion of nucleotide sequences into e. coli chromosome
US5665587A (en) * 1989-06-26 1997-09-09 Novo Nordisk A/S Modified subtilisins and detergent compositions containing same
GB8915658D0 (en) * 1989-07-07 1989-08-23 Unilever Plc Enzymes,their production and use
US5658871A (en) * 1989-07-07 1997-08-19 Lever Brothers Company, Division Of Conopco, Inc. Microbial lipase muteins and detergent compositions comprising same
NL8902010A (nl) * 1989-08-04 1991-03-01 Nl Zuivelonderzoek Inst Dna-fragment met ten minste een protease-gen, een dergelijk dna-fragment bevattende cloneringsvektor, een gastheercel getransformeerd met een dusdanig opgebouwde cloneringsvektor, alsmede de met dergelijke gastheercellen geproduceerde proteasen resp. bereide produkten zoals voedingsmiddelen.
EP0414297B1 (en) * 1989-08-11 1996-10-16 Gist-Brocades N.V. Efficient production of mutant proteases
US5352603A (en) * 1989-08-31 1994-10-04 Kali-Chemie Ag Highly alkaline proteases
DE4023458A1 (de) * 1989-08-31 1991-03-07 Kali Chemie Ag Neue hochalkalische proteasen
US6271012B1 (en) * 1989-10-11 2001-08-07 Genencor International, Inc. Protease muteins and their use in detergents
JPH0813271B2 (ja) * 1989-11-27 1996-02-14 日本ケミカルリサーチ株式会社 線溶活性蛋白質およびその製造法
US5030378A (en) * 1990-01-02 1991-07-09 The Procter & Gamble Company Liquid detergents containing anionic surfactant, builder and proteolytic enzyme
DE69133491T2 (de) * 1990-01-04 2006-07-27 Genencor International, Inc., Palo Alto Glucose-Isomerase mit geändertem pH-Optimum
IT1239733B (it) * 1990-02-23 1993-11-15 Eniricerche Spa Mutanti della proteasi neutra termostabili e mezzi e metodi per la loro preparazione
DK271490D0 (da) * 1990-11-14 1990-11-14 Novo Nordisk As Detergentkomposition
DE4037530A1 (de) * 1990-11-26 1992-05-27 Henkel Kgaa Faellungsverfahren fuer exocellulaere proteine
US5482849A (en) * 1990-12-21 1996-01-09 Novo Nordisk A/S Subtilisin mutants
US5340735A (en) 1991-05-29 1994-08-23 Cognis, Inc. Bacillus lentus alkaline protease variants with increased stability
US5702931A (en) * 1991-07-01 1997-12-30 Berlex Laboratories, Inc. Mutagenesis methods and compositions
US5770410A (en) * 1992-01-30 1998-06-23 Genzyme Corporation Chiral synthesis with modified enzymes
JP3431146B2 (ja) * 1992-01-30 2003-07-28 ジェンザイム・リミテッド 改変した酵素によるキラル合成
US5316935A (en) * 1992-04-06 1994-05-31 California Institute Of Technology Subtilisin variants suitable for hydrolysis and synthesis in organic media
US5869644A (en) * 1992-04-15 1999-02-09 The Johns Hopkins University Synthesis of diverse and useful collections of oligonucleotidies
JPH0657299A (ja) * 1992-05-13 1994-03-01 Procter & Gamble Co:The 陰イオン界面活性剤とカルボキシレートビルダーとタンパク分解酵素とアルカノールアミノとを含有する液体洗剤
WO1994002597A1 (en) * 1992-07-23 1994-02-03 Novo Nordisk A/S MUTANT α-AMYLASE, DETERGENT, DISH WASHING AGENT, AND LIQUEFACTION AGENT
PT867504E (pt) 1993-02-11 2003-08-29 Genencor Int Alfa-amilase estavel a oxidacao
DK39093D0 (da) * 1993-04-01 1993-04-01 Novo Nordisk As Enzym
US6440717B1 (en) * 1993-09-15 2002-08-27 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis
US6436690B1 (en) * 1993-09-15 2002-08-20 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis wherein one or more loop regions are substituted
EP1921147B1 (en) 1994-02-24 2011-06-08 Henkel AG & Co. KGaA Improved enzymes and detergents containing them
ES2364774T3 (es) 1994-02-24 2011-09-14 HENKEL AG & CO. KGAA Enzimas mejoradas y detergentes que las contienen.
USH1468H (en) 1994-04-28 1995-08-01 Costa Jill B Detergent compositions containing cellulase enzyme and selected perfumes for improved odor and stability
US6599730B1 (en) * 1994-05-02 2003-07-29 Procter & Gamble Company Subtilisin 309 variants having decreased adsorption and increased hydrolysis
EP0693549A1 (en) 1994-07-19 1996-01-24 The Procter & Gamble Company Solid bleach activator compositions
PL318209A1 (en) * 1994-08-11 1997-05-26 Genencor Int Improved cleaning composition
GB9416841D0 (en) * 1994-08-19 1994-10-12 Finnfeeds Int Ltd An enzyme feed additive and animal feed including it
US5780285A (en) * 1995-03-03 1998-07-14 Genentech, Inc. Subtilisin variants capable of cleaving substrates containing dibasic residues
US5837516A (en) * 1995-03-03 1998-11-17 Genentech, Inc. Subtilisin variants capable of cleaving substrates containing basic residues
US6455295B1 (en) * 1995-03-08 2002-09-24 The Procter & Gamble Company Subtilisin Carlsberg variants having decreased adsorption and increased hydrolysis
US6475765B1 (en) * 1995-03-09 2002-11-05 Procter & Gamble Company Subtilisin DY variants having decreased adsorption and increased hydrolysis
IL117350A0 (en) * 1995-03-09 1996-07-23 Procter & Gamble Proteinase k variants having decreased adsorption and increased hydrolysis
US6682924B1 (en) * 1995-05-05 2004-01-27 Novozymes A/S Protease variants and compositions
ES2115338T3 (es) 1995-07-13 1998-06-16 Procter & Gamble Composicion de espumacion para envasado.
EP0753567A1 (en) 1995-07-14 1997-01-15 The Procter & Gamble Company Softening through the wash compositions
DE19530816A1 (de) 1995-08-23 1997-02-27 Cognis Bio Umwelt Verwendung von mutierter Subtilisin-Protease in kosmetischen Produkten
EP0778342A1 (en) 1995-12-06 1997-06-11 The Procter & Gamble Company Detergent compositions
AU5725996A (en) 1996-05-03 1997-11-26 Procter & Gamble Company, The Detergent compositions comprising polyamine polymers with improved soil dispersancy
US5858948A (en) * 1996-05-03 1999-01-12 Procter & Gamble Company Liquid laundry detergent compositions comprising cotton soil release polymers and protease enzymes
US5958739A (en) * 1996-06-06 1999-09-28 Genencor International Inc. Mutant α-amylase
US6165966A (en) * 1996-09-24 2000-12-26 The Procter & Gamble Company Liquid detergents containing proteolytic enzyme and protease inhibitors
JP2000506931A (ja) * 1996-09-24 2000-06-06 ザ、プロクター、エンド、ギャンブル、カンパニー タンパク質分解酵素とプロテアーゼインヒビターを含有した液体洗剤
US6180586B1 (en) 1996-09-24 2001-01-30 The Procter & Gamble Company Liquid laundry detergent compositions containing proteolytic enzyme and protease inhibitors
KR19990076798A (ko) * 1996-10-29 1999-10-15 요트.게.아. 롤페즈 텔레비젼 수상기
JP4044143B2 (ja) 1996-11-04 2008-02-06 ノボザイムス アクティーゼルスカブ ズブチラーゼ変異体及び組成物
EP0932667B1 (en) 1996-11-04 2008-10-01 Novozymes A/S Subtilase variants and compositions
BR9714194A (pt) * 1996-12-31 2000-03-28 Procter & Gamble Composições detergentes para a lavagem de tecidos com poliamidas-poliaminas para proporcionar benefìcios na aparência aos tecidos lavados com as memas
BR9714455A (pt) 1996-12-31 2000-03-21 Procter & Gamble e aquosas, de baixo custo com tensoativos aromáticos.
WO1998029527A1 (en) 1996-12-31 1998-07-09 The Procter & Gamble Company Thickened, highly aqueous liquid detergent compositions
US6156722A (en) * 1996-12-31 2000-12-05 The Procter & Gamble Company Laundry detergent compositions comprising dye fixatives
DE19717346C2 (de) * 1997-04-24 1999-05-20 Max Planck Gesellschaft DNA-Sonden, Verfahren und Kit zur Identifizierung Antibiotika-resistenter Bakterienstämme
US6080568A (en) * 1997-08-19 2000-06-27 Genencor International, Inc. Mutant α-amylase comprising modification at residues corresponding to A210, H405 and/or T412 in Bacillus licheniformis
BR9811248B1 (pt) 1997-08-29 2011-10-04 variante de enzima subtilase derivada de uma subtilase originária selecionada a partir do sub-grupo i-s1 ou do sub-grupo i-s2, dita variante tendo melhorado desempenho de lavagem em detergentes em comparação com a subtilase originária, sequência de dna isolada, vetor de expressão, célula hospedeira microbiana, processo para produzir uma variante, composição, uso de uma variante de subtilase.
JP2001514846A (ja) 1997-08-29 2001-09-18 ノボ ノルディスク アクティーゼルスカブ プロテアーゼ変異体及び組成物
EP1032655B1 (en) 1997-11-21 2005-06-29 Novozymes A/S Protease variants and compositions
US6773907B2 (en) 1997-11-21 2004-08-10 Peter Kamp Hansen Subtilase enzymes
US6780629B2 (en) 1997-11-21 2004-08-24 Novozymes A/S Subtilase enzymes
NZ505298A (en) 1997-12-20 2002-10-25 Genencor Int Enzyme (protease) granule formulations with salt hydrate and polymer coating having high solubility and increased stability
CN1242060C (zh) 1997-12-20 2006-02-15 金克克国际有限公司 基体颗粒
EP1057015A1 (en) 1997-12-20 2000-12-06 Genencor International, Inc. Accelerated stability test
EP1021525A1 (en) 1997-12-20 2000-07-26 Genencor International, Inc. Fluidized bed matrix granule
US6908757B1 (en) 1998-03-26 2005-06-21 The Procter & Gamble Company Serine protease variants having amino acid deletions and substitutions
ES2237209T3 (es) 1998-10-27 2005-07-16 Genencor International, Inc. Granulo de matriz.
MXPA01004750A (es) 1998-11-13 2005-07-01 Genencor Int Granulo de baja densidad de lecho fluidizado.
ATE504651T1 (de) 1998-12-18 2011-04-15 Novozymes As Subtilase enzyme der i-s1- und i-s2-untergruppen mit einem zusätzlichen aminosäurerest in einer aktiven schleifenregion
DE60026970T2 (de) 1999-01-08 2006-11-30 Genencor International, Inc., Palo Alto Zusammensetzungen mit niedriger dichte und partikeln der diese enthalten
WO2000071688A1 (en) 1999-05-20 2000-11-30 Novozymes A/S Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additional amino acid residue between positions 126 and 127
ATE408679T1 (de) 1999-05-20 2008-10-15 Novozymes As Subtilase enzyme der i-s1 und i-s2 untergruppen mit mindestens einer zusätzlichen aminosäure zwischen position 127 und 128
AU4393000A (en) 1999-05-20 2000-12-12 Novozymes A/S Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additionalamino acid residue between positions 128 and 129
ATE402996T1 (de) 1999-05-20 2008-08-15 Novozymes As Subtilase-enzyme der i-s1 und i-s2 untergruppen mit wenigstens einem zusätzlichen aminosäurerest zwischen positionen 125 und 126
ATE408676T1 (de) 1999-05-20 2008-10-15 Novozymes As Subtilase enzyme der i-s1 und i-s2 untergruppen mit mindestens einer zusätzlichen aminosäure zwischen position 132 und 133
DE60040284D1 (de) 1999-05-20 2008-10-30 Novozymes As Subtilase enzyme der i-s1 und i-s2 untergruppen mit mindestens einer zusätzlichen aminosäure zwischen position 129 und 130
BR0012660A (pt) 1999-07-22 2002-04-09 Procter & Gamble Variante de protease tipo subtilisina; composição de limpeza; e composição de cuidado pessoal
MXPA02000842A (es) 1999-07-22 2002-07-30 Procter & Gamble Conjugados de proteasa que tienen sitios de corte protegidos estericamente.
US6946128B1 (en) 1999-07-22 2005-09-20 The Procter & Gamble Company Protease conjugates having sterically protected epitope regions
CN1373802A (zh) 1999-07-22 2002-10-09 宝洁公司 在特定表位区域具有氨基酸缺失和取代的枯草杆菌蛋白酶变体
US7217554B2 (en) 1999-08-31 2007-05-15 Novozymes A/S Proteases and variants thereof
WO2001016285A2 (en) 1999-08-31 2001-03-08 Novozymes A/S Novel proteases and variants thereof
US6558939B1 (en) 1999-08-31 2003-05-06 Novozymes, A/S Proteases and variants thereof
BR9917481A (pt) * 1999-09-09 2002-05-21 Procter & Gamble Composição detergente contendo uma protease
CN1398235A (zh) 1999-09-22 2003-02-19 宝洁公司 手持液体容器
JP2003514922A (ja) 1999-10-15 2003-04-22 ジェネンコア インターナショナル インコーポレーテッド 蛋白質含有顆粒および顆粒配合物
US6727085B2 (en) * 1999-12-15 2004-04-27 Fanoe Tina Sejersgaard Subtilase variants having an improved wash performance on egg stains
US6960462B2 (en) 2000-02-08 2005-11-01 Dsm Ip Assets B.V Use of acid-stable subtilisin proteases in animal feed
US6855548B2 (en) 2000-02-08 2005-02-15 F. Hoffman-La Roche Ag Use of acid-stable proteases in animal feed
US6777218B1 (en) 2000-03-14 2004-08-17 Novozymes A/S Subtilase enzymes having an improved wash performance on egg stains
EP1328612B1 (en) 2000-10-27 2007-04-25 Genencor International, Inc. Particle with substituted polyvinyl alcohol coating
US6803222B2 (en) * 2000-11-22 2004-10-12 Kao Corporation Alkaline proteases
PL210859B1 (pl) 2001-03-23 2012-03-30 Genencor Int Odmiana subtylizyny zawierająca zmieniony epitop limfocytów T, kwas nukleinowy kodujący tę odmianę subtylizyny, wektor ekspresyjny i komórka gospodarza zawierająca kwas nukleinowy oraz kompozycje zawierające tę odmianę subtylizyny
US8076113B2 (en) 2001-04-02 2011-12-13 Danisco Us Inc. Method for producing granules with reduced dust potential comprising an antifoam agent
US7157416B2 (en) 2001-07-20 2007-01-02 Genencor International, Inc. Stabilization of enzymes
US7153820B2 (en) 2001-08-13 2006-12-26 Ecolab Inc. Solid detergent composition and method for solidifying a detergent composition
DE10153792A1 (de) 2001-10-31 2003-05-22 Henkel Kgaa Neue Alkalische Protease-Varianten und Wasch- und Reinigungsmittel enthaltend diese neuen Alkalischen Protease-Varianten
DE10162727A1 (de) 2001-12-20 2003-07-10 Henkel Kgaa Neue Alkalische Protease aus Bacillus gibsonii (DSM 14391) und Wasch-und Reinigungsmittel enthaltend diese neue Alkalische Protease
DE10162728A1 (de) 2001-12-20 2003-07-10 Henkel Kgaa Neue Alkalische Protease aus Bacillus gibsonii (DSM 14393) und Wasch-und Reinigungsmittel enthaltend diese neue Alkalische Protease
DE10163884A1 (de) 2001-12-22 2003-07-10 Henkel Kgaa Neue Alkalische Protease aus Bacillus sp. (DSM 14392) und Wasch- und Reinigungsmittel enthaltend diese neue Alkalische Protease
US20050054843A1 (en) * 2001-12-31 2005-03-10 Estell David A Proteases producing an altered immunological response and methods of making and using the same
JP2005535284A (ja) * 2001-12-31 2005-11-24 ジェネンコー・インターナショナル・インク 免疫反応の変化を生じるプロテアーゼ、ならびにその製造および利用方法
DK1495128T3 (da) * 2002-03-29 2014-08-11 Genencor Int Forstærket proteinekspression i Bacillus
EP2431048B1 (en) 2002-10-08 2015-03-11 Danisco US Inc. Phenolic binding peptides
US7888093B2 (en) * 2002-11-06 2011-02-15 Novozymes A/S Subtilase variants
DE602004007766T2 (de) 2003-02-07 2008-06-05 Novozymes A/S Proteasen
JP4819670B2 (ja) 2003-05-07 2011-11-24 ノボザイムス アクティーゼルスカブ 変異体スブチリシン酵素（サブチラーゼ）
DE602004030595D1 (de) 2003-10-10 2011-01-27 Novozymes As Proteasevarianten
ATE516347T1 (de) 2003-10-23 2011-07-15 Novozymes As Protease mit verbesserter stabilität in detergentien
BRPI0511171A (pt) 2004-05-17 2007-12-04 Procter & Gamble composição de alvejamento compreendendo um carboidrato oxidase
ES2380105T3 (es) 2004-06-21 2012-05-08 Novozymes A/S Proteasas de Nocardiopsis
JP4681283B2 (ja) 2004-11-18 2011-05-11 独立行政法人海洋研究開発機構 新規な高アルカリプロテアーゼ及びその利用
JP4485341B2 (ja) 2004-12-20 2010-06-23 花王株式会社 組換え微生物
EP1700904A1 (en) 2005-03-11 2006-09-13 Unilever N.V. Liquid detergent composition
EP1700907A1 (en) 2005-03-11 2006-09-13 Unilever N.V. Liquid bleaching composition
US7666963B2 (en) 2005-07-21 2010-02-23 Akzo Nobel N.V. Hybrid copolymers
WO2011017223A1 (en) 2009-07-31 2011-02-10 Akzo Nobel N.V. Hybrid copolymer compositions for personal care applications
PT2007942E (pt) 2006-04-14 2014-10-07 Danisco Us Inc Tratamento de têxteis num passo
US20090215663A1 (en) 2006-04-20 2009-08-27 Novozymes A/S Savinase variants having an improved wash performance on egg stains
US20080020961A1 (en) 2006-07-21 2008-01-24 Rodrigues Klin A Low Molecular Weight Graft Copolymers
NO20073834L (no) 2006-07-21 2008-01-22 Akzo Nobel Chemicals Int Bv Sulfonerte podede kopolymerer
US8093200B2 (en) 2007-02-15 2012-01-10 Ecolab Usa Inc. Fast dissolving solid detergent
WO2008134343A1 (en) 2007-04-30 2008-11-06 Danisco Us Inc., Genencor Division Use of protein hydrolysates to stabilize metalloprotease detergent formulations
SG148934A1 (en) 2007-06-11 2009-01-29 Novozymes As A process for combined biopolishing and bleach clean-up
WO2009009493A2 (en) 2007-07-09 2009-01-15 THE UNITED STATES OF AMERICA, as represented by THE SECRETARY, DEPARTEMENT OF HEALTH AND HUMAN SERVICES Aegyptin and uses thereof
WO2009050684A2 (en) 2007-10-18 2009-04-23 Ecolab Inc. Pressed, waxy, solid cleaning compositions and methods of making them
DK2205731T3 (da) * 2007-10-31 2013-03-11 Danisco Us Inc Anvendelse og fremstilling af citrat-stabile, neutrale metalloproteaser
MX346974B (es) 2008-02-14 2017-04-07 Danisco Us Inc Granulos pequeños que contienen enzimas.
US8293697B2 (en) 2009-03-18 2012-10-23 The Procter & Gamble Company Structured fluid detergent compositions comprising dibenzylidene sorbitol acetal derivatives
US8153574B2 (en) 2009-03-18 2012-04-10 The Procter & Gamble Company Structured fluid detergent compositions comprising dibenzylidene polyol acetal derivatives and detersive enzymes
WO2010138347A1 (en) 2009-05-26 2010-12-02 The Procter & Gamble Company Aqueous liquid composition for pre-treating soiled dishware
DE102009027045A1 (de) * 2009-06-19 2010-12-30 Henkel Ag & Co. Kgaa Neue Proteasen und Mittel enthaltend diese Proteasen
WO2011005623A1 (en) 2009-07-09 2011-01-13 The Procter & Gamble Company Laundry detergent composition comprising low level of bleach
US20110009307A1 (en) 2009-07-09 2011-01-13 Alan Thomas Brooker Laundry Detergent Composition Comprising Low Level of Sulphate
KR101802988B1 (ko) 2010-05-14 2017-12-14 헨켈 아이피 앤드 홀딩 게엠베하 중합체 함유 세정 조성물, 이의 제조 방법 및 용도
US20130224757A1 (en) * 2010-08-19 2013-08-29 Novozymes A/S Induced sporulation screening method
JP6023180B2 (ja) 2011-05-20 2016-11-09 エコラボ ユーエスエー インコーポレイティド 物品洗浄のための、アルカリ／酸交替系における非ホスフェート洗剤及び非リン酸
CA2856820C (en) 2011-05-20 2019-10-29 Ecolab Usa Inc. Acid formulations for use in a system for warewashing
US8853144B2 (en) 2011-08-05 2014-10-07 Ecolab Usa Inc. Cleaning composition containing a polysaccharide graft polymer composition and methods of improving drainage
US8636918B2 (en) 2011-08-05 2014-01-28 Ecolab Usa Inc. Cleaning composition containing a polysaccharide hybrid polymer composition and methods of controlling hard water scale
US8679366B2 (en) 2011-08-05 2014-03-25 Ecolab Usa Inc. Cleaning composition containing a polysaccharide graft polymer composition and methods of controlling hard water scale
US8841246B2 (en) 2011-08-05 2014-09-23 Ecolab Usa Inc. Cleaning composition containing a polysaccharide hybrid polymer composition and methods of improving drainage
JP2014532791A (ja) 2011-11-04 2014-12-08 アクゾ ノーベル ケミカルズ インターナショナル ベスローテン フエンノートシャップＡｋｚｏ Ｎｏｂｅｌ Ｃｈｅｍｉｃａｌｓ Ｉｎｔｅｒｎａｔｉｏｎａｌ Ｂ．Ｖ． ハイブリッド樹状コポリマー、その組成物及びそれを製造する方法
US9051406B2 (en) 2011-11-04 2015-06-09 Akzo Nobel Chemicals International B.V. Graft dendrite copolymers, and methods for producing the same
WO2013088266A1 (en) 2011-12-13 2013-06-20 Ecolab Usa Inc. Concentrated warewashing compositions and methods
JP6040987B2 (ja) * 2012-07-06 2016-12-07 東洋紡株式会社 改変型アルカリホスファターゼ
WO2014018309A1 (en) 2012-07-26 2014-01-30 The Procter & Gamble Company Low ph liquid cleaning compositions with enzymes
US8945314B2 (en) 2012-07-30 2015-02-03 Ecolab Usa Inc. Biodegradable stability binding agent for a solid detergent
US9745543B2 (en) 2012-09-10 2017-08-29 Ecolab Usa Inc. Stable liquid manual dishwashing compositions containing enzymes
WO2014055107A1 (en) 2012-10-04 2014-04-10 Ecolab Usa Inc. Pre-soak technology for laundry and other hard surface cleaning
US9139458B2 (en) 2013-03-15 2015-09-22 Janet Angel Compositions and methods of use
CA2921800C (en) 2013-09-09 2018-10-02 Ecolab Usa Inc. Synergistic stain removal through novel chelator combination
MX394036B (es) 2013-09-09 2025-03-24 Ecolab Usa Inc Eliminacion sinergica de manchas a traves de una combinacion de quelacion novedosa.
MX395198B (es) 2013-10-24 2025-03-25 Ecolab Usa Inc Composiciones y metodos para eliminar suciedades de las superficies.
US9365805B2 (en) 2014-05-15 2016-06-14 Ecolab Usa Inc. Bio-based pot and pan pre-soak
EP3174446B1 (en) 2014-08-01 2019-01-30 Ecolab USA Inc. A method of manual surface cleaning using cleaning textiles and of washing said cleaning textiles
US10577571B2 (en) 2016-11-08 2020-03-03 Ecolab Usa Inc. Non-aqueous cleaner for vegetable oil soils
WO2020099491A1 (en) 2018-11-14 2020-05-22 Novozymes A/S Oral care composition comprising a polypeptide having dnase activity
CN114207101B (zh) 2019-07-31 2024-05-31 埃科莱布美国股份有限公司 免用个人防护设备的脱灰剂组合物
US11873465B2 (en) 2019-08-14 2024-01-16 Ecolab Usa Inc. Methods of cleaning and soil release of highly oil absorbing substrates employing optimized extended chain nonionic surfactants
CN110760466B (zh) * 2019-11-18 2021-04-20 山东隆科特酶制剂有限公司 一株产耐高温中性蛋白酶的枯草芽孢杆菌及其应用
WO2022010893A1 (en) 2020-07-06 2022-01-13 Ecolab Usa Inc. Foaming mixed alcohol/water compositions comprising a combination of alkyl siloxane and a hydrotrope/solubilizer
WO2022010906A1 (en) 2020-07-06 2022-01-13 Ecolab Usa Inc. Peg-modified castor oil based compositions for microemulsifying and removing multiple oily soils
CA3185062A1 (en) 2020-07-06 2022-01-13 Gang Pu Foaming mixed alcohol/water compositions comprising a structured alkoxylated siloxane
US20230332124A1 (en) 2020-08-24 2023-10-19 Novozymes A/S Oral care composition comprising a fructanase
CN117677689A (zh) 2021-05-18 2024-03-08 诺力昂化学品国际有限公司 在清洁应用中的聚酯聚季铵盐
EP4341317A1 (en) 2021-05-20 2024-03-27 Nouryon Chemicals International B.V. Manufactured polymers having altered oligosaccharide or polysaccharide functionality or narrowed oligosaccharide distribution, processes for preparing them, compositions containing them, and methods of using them
WO2023275269A1 (en) 2021-06-30 2023-01-05 Nouryon Chemicals International B.V. Chelate-amphoteric surfactant liquid concentrates and use thereof in cleaning applications
WO2023057367A1 (en) 2021-10-08 2023-04-13 Unilever Ip Holdings B.V. Laundry composition
EP4558596A1 (en) 2022-07-20 2025-05-28 Ecolab USA Inc. Novel nonionic extended surfactants, compositions and methods of use thereof

Family Cites Families (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US362957A (en) * 1887-05-17 haig-ht
US3557002A (en) 1967-11-15 1971-01-19 Procter & Gamble Stabilized aqueous enzyme preparation
US3558498A (en) 1967-11-29 1971-01-26 Procter & Gamble Granular detergent composition containing enzymes and environmental control components
GB1240058A (en) 1968-04-12 1971-07-21 Procter & Gamble Enzyme-containing detergent compositions
US3623957A (en) 1970-01-21 1971-11-30 Baxter Laboratories Inc Preparation of microbial alkaline protease by fermentation with bacillus subtilis, variety licheniformis
BE785666A (fr) 1971-07-01 1973-01-02 Procter & Gamble Procede pour la production de compositions enzymatiques en granules
BE792982A (en) 1971-12-20 1973-06-19 Procter & Gamble Europ Proteolytic enzymes detergent - contg cationic and anionic surfactants
US4011169A (en) 1973-06-29 1977-03-08 The Procter & Gamble Company Stabilization and enhancement of enzymatic activity
IT1106254B (it) 1976-03-08 1985-11-11 Procter & Gamble Europ Composizione detergente liquida contenente enzimi
US4090973A (en) 1976-06-24 1978-05-23 The Procter & Gamble Company Method for making stable detergent compositions
DE2633601A1 (de) 1976-07-27 1978-02-02 Henkel Kgaa Fluessiges, als wasch- und reinigungsmittel verwendbares, enzymhaltiges konzentrat
GB1603640A (en) 1977-07-20 1981-11-25 Gist Brocades Nv Enzyme particles
US4261868A (en) 1979-08-08 1981-04-14 Lever Brothers Company Stabilized enzymatic liquid detergent composition containing a polyalkanolamine and a boron compound
US4302544A (en) * 1979-10-15 1981-11-24 University Of Rochester Asporogenous mutant of B. subtilis for use as host component of HV1 system
US4318818A (en) 1979-11-09 1982-03-09 The Procter & Gamble Company Stabilized aqueous enzyme composition
JPS5950280B2 (ja) 1980-10-24 1984-12-07 花王株式会社 酵素入り漂白剤組成物
JPS5786299A (en) * 1980-11-19 1982-05-29 Dai Ichi Seiyaku Co Ltd Plasmid and its preparation
CA1170202A (en) * 1981-01-15 1984-07-03 Susan Mickel Process for cloning the gene coding for a thermostable alpha-amylase into escherichia coli and bacillus subtilis
US4404128A (en) 1981-05-29 1983-09-13 The Procter & Gamble Company Enzyme detergent composition
DE3122216A1 (de) * 1981-06-04 1982-12-23 Boehringer Mannheim Gmbh, 6800 Mannheim Verfahren zur herstellung eines mikroorganismus, welcher (alpha)-galactosidase, aber keine invertase bildet, so erhaltener mikroorganismus und seine verwendung
EP0080748B1 (en) 1981-11-13 1985-07-10 Unilever N.V. Enzymatic liquid cleaning composition
JPS58162291A (ja) * 1982-03-23 1983-09-26 Res Dev Corp Of Japan 耐熱性プロテア−ゼの製造法
FI88932C (fi) * 1982-04-15 1993-07-26 Genentech Inc Framstaellning av funktionellt maenskligt urokinasprotein
JPS5959190A (ja) * 1982-09-29 1984-04-04 Agency Of Ind Science & Technol プロテア−ゼの製造法
DK135983D0 (da) * 1983-03-25 1983-03-25 Novo Industri As Maltogen amylaseenzymprodukt og fremgangsmade til dets fremstilling og anvendelse
NZ208612A (en) 1983-06-24 1991-09-25 Genentech Inc Method of producing "procaryotic carbonyl hydrolases" containing predetermined, site specific mutations

Also Published As

Publication number Publication date
DE3486139D1 (da) 1993-06-03
JPH06319534A (ja) 1994-11-22
ATE60797T1 (de) 1991-02-15
JP2594533B2 (ja) 1997-03-26
EP0130756B2 (en) 2000-06-28
EP0247647B1 (en) 1991-01-23
NZ208612A (en) 1991-09-25
EP0130756A1 (en) 1985-01-09
EP0357157A3 (en) 1990-03-28
HK99293A (en) 1993-09-30
DE3486139T2 (de) 1993-11-11
JPS6070075A (ja) 1985-04-20
DK175768B1 (da) 2005-02-14
HK75493A (en) 1993-08-06
DE3484019D1 (de) 1991-02-28
DK175278B1 (da) 2004-08-02
EP0130756B1 (en) 1991-02-06
DE3484079D1 (de) 1991-03-14
EP0357157A2 (en) 1990-03-07
EP0246678B1 (en) 1993-04-28
DK305984A (da) 1985-02-18
DK305984D0 (da) 1984-06-22
DK82393D0 (da) 1993-07-08
EP0246678A1 (en) 1987-11-25
DK82293D0 (da) 1993-07-08
DK82393A (da) 1993-07-08
HK75393A (en) 1993-08-06
IE81141B1 (en) 2000-04-05
ATE60356T1 (de) 1991-02-15
JP2889095B2 (ja) 1999-05-10
EP0247647A1 (en) 1987-12-02
JPH06315378A (ja) 1994-11-15
IE841567L (en) 1984-12-24

Similar Documents

Publication Publication Date Title
DK82293D0 (da) Normalt sporulerende bacillus uden evne til at udskille subtilisin eller neutral protease, kulturer deraf og fremgangsmaade til fremstilling af heterologe proteiner dermed
ATE144281T1 (de) Hefezellen der gattung-schwanniomyces
BG89942A (bg) Метод за получаване на протеолитични ензими
FI906442A0 (fi) Traodsvamp som saknar asparaginproteinas.
ATE320495T1 (de) Kalziumfreie subtilisin mutanten
IE880558L (en) Stable gene amplification in prokaryotic chromosomal dna
DK1117808T3 (da) Transformaationssystem inden jfjor området filamentöse svampeværter: I chrysosporium
YU46723B (sh) Ekspresija, prerada i sekrecija heterologog proteina pomoću kvasca
BG49049A3 (bg) Метод за клониране и експресия на гени, кодиращи протеолитични ензими
NO885391L (no) Nye proteolytiske enzymer.
DE60321901D1 (de) Verbesserte bacillus-wirtszelle
PT83714B (pt) Processo para a preparacao de plasmideos de clonagem e de analise da regiao de regulacao de bacillus
ATE108831T1 (de) Rinder-interleukin-1-alpha.
CN101978056B (zh) 质粒载体
FR2603302A1 (fr) Nouvelles souches de bacillus brevis et leur application
DE60335703D1 (de) Verbesserte proteinexpression in bacillus subtilis
ATE292683T1 (de) Erhöhung der proteinproduktion in gram-positiven mikroorganismen
ATE78870T1 (de) Verfahren zur herstellung von neutraler protease.
IT1186750B (it) Vettore di clonaggio,molecole di dna ricombinante,ceppi di bacillus subtilis trasformati con dette molecole e metodi per l'espressione di geni eterologhi e produzione e secrezione di proteine codificate da detti geni
DK0592574T3 (da) Fremgangsmåde til fremstilling af rekombinante proteiner og værtsceller anvendt derved
ES8608578A1 (es) Procedimiento para la sintesis de una proteina deseada se- leccionada del grupo de amilasa

Legal Events

Date Code Title Description
A0 Application filed
PUP Patent expired

Expiry date: 20130708