ATE60797T1 - Prokaryotische carbonyl-hydrolasen, verfahren, dna, vektoren und transformierte wirte zu ihrer herstellung und diese hydrolasen enthaltende detergenszusammensetzungen. - Google Patents

Prokaryotische carbonyl-hydrolasen, verfahren, dna, vektoren und transformierte wirte zu ihrer herstellung und diese hydrolasen enthaltende detergenszusammensetzungen.

Info

Publication number
ATE60797T1
ATE60797T1 AT84304252T AT84304252T ATE60797T1 AT E60797 T1 ATE60797 T1 AT E60797T1 AT 84304252 T AT84304252 T AT 84304252T AT 84304252 T AT84304252 T AT 84304252T AT E60797 T1 ATE60797 T1 AT E60797T1
Authority
AT
Austria
Prior art keywords
dna
hydrolase
prokaryotic
vectors
production
Prior art date
Application number
AT84304252T
Other languages
English (en)
Inventor
Richard Ray Bott
Eugenio Ferrari
James Allen Wells
David Aaron Estell
Dennis James Henner
Original Assignee
Genentech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=27560079&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ATE60797(T1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority claimed from US06/614,612 external-priority patent/US4760025A/en
Application filed by Genentech Inc filed Critical Genentech Inc
Application granted granted Critical
Publication of ATE60797T1 publication Critical patent/ATE60797T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07—Bacillus

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Detergent Compositions (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT84304252T 1983-06-24 1984-06-22 Prokaryotische carbonyl-hydrolasen, verfahren, dna, vektoren und transformierte wirte zu ihrer herstellung und diese hydrolasen enthaltende detergenszusammensetzungen. ATE60797T1 (de)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US50741983A 1983-06-24 1983-06-24
US61461784A 1984-05-29 1984-05-29
US61461584A 1984-05-29 1984-05-29
US61449184A 1984-05-29 1984-05-29
US61461684A 1984-05-29 1984-05-29
US06/614,612 US4760025A (en) 1984-05-29 1984-05-29 Modified enzymes and methods for making same
EP84304252A EP0130756B2 (de) 1983-06-24 1984-06-22 Prokaryotische Carbonyl-Hydrolasen, Verfahren, DNA, Vektoren und transformierte Wirte zu ihrer Herstellung und diese Hydrolasen enthaltende Detergenszusammensetzungen

Publications (1)

Publication Number Publication Date
ATE60797T1 true ATE60797T1 (de) 1991-02-15

Family

ID=27560079

Family Applications (2)

Application Number Title Priority Date Filing Date
AT84304252T ATE60797T1 (de) 1983-06-24 1984-06-22 Prokaryotische carbonyl-hydrolasen, verfahren, dna, vektoren und transformierte wirte zu ihrer herstellung und diese hydrolasen enthaltende detergenszusammensetzungen.
AT87200689T ATE60356T1 (de) 1983-06-24 1984-06-22 Verfahren zur mutagenese von dns.

Family Applications After (1)

Application Number Title Priority Date Filing Date
AT87200689T ATE60356T1 (de) 1983-06-24 1984-06-22 Verfahren zur mutagenese von dns.

Country Status (8)

Country Link
EP (4) EP0247647B1 (de)
JP (3) JP2594533B2 (de)
AT (2) ATE60797T1 (de)
DE (3) DE3486139T2 (de)
DK (3) DK175278B1 (de)
HK (3) HK75393A (de)
IE (1) IE81141B1 (de)
NZ (1) NZ208612A (de)

Families Citing this family (224)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ208612A (en) 1983-06-24 1991-09-25 Genentech Inc Method of producing "procaryotic carbonyl hydrolases" containing predetermined, site specific mutations
CA1311429C (en) * 1983-07-06 1992-12-15 Vasantha Nagarajan Vector for polypeptides in bacilli
JPH0659224B2 (ja) * 1983-09-12 1994-08-10 工業技術院長 Ｄνａ塩基配列及びそのｄνａ塩基配列を含む組換え体プラスミドの創製法
JPH07106156B2 (ja) * 1983-10-28 1995-11-15 ジェネティックス、インスティチュ−ト ファクタ−▲ｖｉｉｉ▼および関連生産物の製造
EP0462632A1 (de) * 1983-10-28 1991-12-27 Genetics Institute, Inc. Verfahren zur Herstellung von gewünschten menschlichen Proteinen durch Rekombinant-DNA-Techniken
EP0149241B1 (de) * 1983-12-28 1991-03-27 Agency Of Industrial Science And Technology DNS-Basensequenz enthaltende Regionen einbegriffen in der Herstellung und Sekretion eines Proteins, rekombinante DNS das Ganze oder einen Teil der DNS-Basensequenz enthaltend und Verfahren zur Herstellung von Proteinen unter Verwendung der rekombinanten DNS
US5411873A (en) * 1984-05-29 1995-05-02 Genencor, Inc. Process for producing heterologous polypeptides
US5972682A (en) * 1984-05-29 1999-10-26 Genencor International, Inc. Enzymatically active modified subtilisins
US5185258A (en) 1984-05-29 1993-02-09 Genencor International, Inc. Subtilisin mutants
US4801537A (en) * 1984-06-08 1989-01-31 Genex Corporation Vector for expression of polypeptides in bacilli
US5840522A (en) * 1984-09-20 1998-11-24 Chiron Corporation Recombinant lectins
US4828994A (en) * 1984-09-21 1989-05-09 Genex Corporation Bacillus strains with reduced extracellular protease levels
CA1340265C (en) * 1985-01-18 1998-12-15 Kirston E. Koths Oxidation resistant muteins
US4752585A (en) * 1985-12-17 1988-06-21 Cetus Corporation Oxidation-resistant muteins
US6084073A (en) * 1985-03-25 2000-07-04 Chiron Corporation Recombinant ricin toxin
EP0316018A3 (de) * 1985-03-29 1989-07-26 Cetus Oncology Corporation Modifikation von DNS-Sequenzen
ATE77648T1 (de) * 1985-04-15 1992-07-15 Procter & Gamble Fluessige reinigungsmittel mit einer anionischen oberflaechenaktiven verbindung, einem verstaerker und einem proteolytischen enzym.
ATE77649T1 (de) * 1985-04-15 1992-07-15 Procter & Gamble Fluessige reinigungsmittel mit einer oberflaechenaktiven verbindung, einem proteolytischen enzym und borsaeure.
IT1186750B (it) * 1985-07-10 1987-12-16 Eniricerche Spa Vettore di clonaggio,molecole di dna ricombinante,ceppi di bacillus subtilis trasformati con dette molecole e metodi per l'espressione di geni eterologhi e produzione e secrezione di proteine codificate da detti geni
DE3527913A1 (de) * 1985-08-03 1987-02-12 Henkel Kgaa Alkalische protease, verfahren zur herstellung von hybridvektoren und genetisch transformierte mikroorganismen
WO1987004461A1 (en) * 1986-01-15 1987-07-30 Amgen THERMALLY STABLE AND pH STABLE SUBTILISIN ANALOGS AND METHOD FOR PRODUCTION THEREOF
US4908773A (en) * 1987-04-06 1990-03-13 Genex Corporation Computer designed stabilized proteins and method for producing same
US5013657A (en) * 1988-04-12 1991-05-07 Bryan Philip N Subtilisin mutations
SG30639G (en) * 1986-04-30 1995-09-01 Genencor Int Non-human carbonyl hydrolase mutants DNA sequences and vectors encoding same and hosts transformed with said vectors
EP0245051B1 (de) * 1986-05-06 1992-08-12 The Board Of Trustees Of The Leland Stanford Junior University Cytotoxische Zusammensetzung aus "Killer"-Zellen
US5364554A (en) * 1986-06-09 1994-11-15 The Clorox Company Proteolytic perhydrolysis system and method of use for bleaching
DE3683910D1 (de) * 1986-09-05 1992-03-26 Cetus Corp Oxidationsresistente muteine von beta-interferon, deren herstellung und diese muteine enthaltende praeparate.
NZ221627A (en) * 1986-09-09 1993-04-28 Genencor Inc Preparation of enzymes, modifications, catalytic triads to alter ratios or transesterification/hydrolysis ratios
EP0283075B1 (de) * 1987-02-27 1999-05-12 Genencor International, Inc. Molekulare Klonierung und Expression von proteolytische Enzyme kodierenden Genen
PT86864B (pt) * 1987-02-27 1992-05-29 Gist Brocades Nv Processo para clonagem molecular e expressao de genes que codificam para enzimas proteoliticas
AU620326B2 (en) * 1987-02-27 1992-02-20 Dsm Ip Assets B.V. Stable gene amplification in chromosomal dna of prokaryotic microorganisms
EP0285123A3 (de) * 1987-04-03 1989-02-01 Stabra AG Verfahren zur vollständigen Mutagenese von Nucleinsäuren
EP0353250B1 (de) * 1987-04-06 1999-09-08 Novo Nordisk A/S Regelung von elektrostatischen interaktionen an metallionen-bindungsstellen zur stabilisierung von proteinen
US4853871A (en) * 1987-04-06 1989-08-01 Genex Corporation Computer-based method for designing stablized proteins
US4914031A (en) * 1987-04-10 1990-04-03 Amgen, Inc. Subtilisin analogs
IT1228925B (it) * 1987-08-07 1991-07-10 Eniricerche Spa Procedimento per la preparazione dell'ormone della crescita umano
US5041378A (en) * 1987-08-11 1991-08-20 Cetus Corporation Procaryotic xylose isomerase muteins
US4939666A (en) * 1987-09-02 1990-07-03 Genex Corporation Incremental macromolecule construction methods
US4966846A (en) * 1987-10-01 1990-10-30 W. R. Grace & Co.-Conn. Molecular cloning and expression of a vibrio proteolyticus neutral protease gene
DK571587D0 (da) * 1987-11-02 1987-11-02 Novo Industri As Enzymatisk detergentsammensaetning
GB8727772D0 (en) * 1987-11-27 1987-12-31 Celltech Ltd Host cells
US4865983A (en) * 1987-12-04 1989-09-12 W. R. Grace & Co.-Conn. Cleaning compositions containing protease produced by vibrio and method of use
DE3877337T2 (de) * 1987-12-11 1993-08-19 Abbott Lab Verfahren zur mutagenese mittels oligonukleotid-gerichteter reparatur eines strandbruchs.
DK6488D0 (da) * 1988-01-07 1988-01-07 Novo Industri As Enzymer
US6287841B1 (en) 1988-02-11 2001-09-11 Genencor International, Inc. High alkaline serine protease
US5324653A (en) * 1988-02-11 1994-06-28 Gist-Brocades N.V. Recombinant genetic means for the production of serine protease muteins
PT89702B (pt) * 1988-02-11 1994-04-29 Gist Brocades Nv Processo para a preparacao de novos enzimas proteoliticos e de detergentes que os contem
US5246849A (en) * 1988-04-12 1993-09-21 Enzon, Inc. Thermally stable serine proteases
US5118623A (en) * 1988-05-27 1992-06-02 Solvay Enzymes, Inc. Bleach stable enzymes
CA1333777C (en) * 1988-07-01 1995-01-03 Randy M. Berka Aspartic proteinase deficient filamentous fungi
HU200796B (en) * 1988-07-14 1990-08-28 Richter Gedeon Vegyeszet Process for producing expression vectors ensuring insertion of nucleotide sequences into e. coli chromosome
US5665587A (en) * 1989-06-26 1997-09-09 Novo Nordisk A/S Modified subtilisins and detergent compositions containing same
US5658871A (en) * 1989-07-07 1997-08-19 Lever Brothers Company, Division Of Conopco, Inc. Microbial lipase muteins and detergent compositions comprising same
GB8915658D0 (en) * 1989-07-07 1989-08-23 Unilever Plc Enzymes,their production and use
NL8902010A (nl) * 1989-08-04 1991-03-01 Nl Zuivelonderzoek Inst Dna-fragment met ten minste een protease-gen, een dergelijk dna-fragment bevattende cloneringsvektor, een gastheercel getransformeerd met een dusdanig opgebouwde cloneringsvektor, alsmede de met dergelijke gastheercellen geproduceerde proteasen resp. bereide produkten zoals voedingsmiddelen.
ATE144283T1 (de) * 1989-08-11 1996-11-15 Gist Brocades Nv Ergiebige herstellung von protease-mutanten
US5352603A (en) * 1989-08-31 1994-10-04 Kali-Chemie Ag Highly alkaline proteases
DE4023458A1 (de) * 1989-08-31 1991-03-07 Kali Chemie Ag Neue hochalkalische proteasen
US6271012B1 (en) * 1989-10-11 2001-08-07 Genencor International, Inc. Protease muteins and their use in detergents
JPH0813271B2 (ja) * 1989-11-27 1996-02-14 日本ケミカルリサーチ株式会社 線溶活性蛋白質およびその製造法
US5030378A (en) * 1990-01-02 1991-07-09 The Procter & Gamble Company Liquid detergents containing anionic surfactant, builder and proteolytic enzyme
DE69133491T2 (de) * 1990-01-04 2006-07-27 Genencor International, Inc., Palo Alto Glucose-Isomerase mit geändertem pH-Optimum
IT1239733B (it) * 1990-02-23 1993-11-15 Eniricerche Spa Mutanti della proteasi neutra termostabili e mezzi e metodi per la loro preparazione
DK271490D0 (da) * 1990-11-14 1990-11-14 Novo Nordisk As Detergentkomposition
DE4037530A1 (de) * 1990-11-26 1992-05-27 Henkel Kgaa Faellungsverfahren fuer exocellulaere proteine
US5482849A (en) * 1990-12-21 1996-01-09 Novo Nordisk A/S Subtilisin mutants
US5340735A (en) * 1991-05-29 1994-08-23 Cognis, Inc. Bacillus lentus alkaline protease variants with increased stability
WO1993001282A1 (en) * 1991-07-01 1993-01-21 Berlex Laboratories, Inc. Novel mutagenesis methods and compositions
JP3431146B2 (ja) * 1992-01-30 2003-07-28 ジェンザイム・リミテッド 改変した酵素によるキラル合成
US5770410A (en) * 1992-01-30 1998-06-23 Genzyme Corporation Chiral synthesis with modified enzymes
US5316935A (en) * 1992-04-06 1994-05-31 California Institute Of Technology Subtilisin variants suitable for hydrolysis and synthesis in organic media
US5869644A (en) * 1992-04-15 1999-02-09 The Johns Hopkins University Synthesis of diverse and useful collections of oligonucleotidies
JPH0657299A (ja) * 1992-05-13 1994-03-01 Procter & Gamble Co:The 陰イオン界面活性剤とカルボキシレートビルダーとタンパク分解酵素とアルカノールアミノとを含有する液体洗剤
WO1994002597A1 (en) * 1992-07-23 1994-02-03 Novo Nordisk A/S MUTANT α-AMYLASE, DETERGENT, DISH WASHING AGENT, AND LIQUEFACTION AGENT
EP0689589B2 (de) * 1993-02-11 2009-09-02 Genencor International, Inc. Oxidativ stabile alpha-amylase
DK39093D0 (da) * 1993-04-01 1993-04-01 Novo Nordisk As Enzym
US6440717B1 (en) * 1993-09-15 2002-08-27 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis
US6436690B1 (en) * 1993-09-15 2002-08-20 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis wherein one or more loop regions are substituted
ATE512227T1 (de) 1994-02-24 2011-06-15 Henkel Ag & Co Kgaa Verbesserte enzyme und detergentien damit
ES2364776T3 (es) * 1994-02-24 2011-09-14 HENKEL AG & CO. KGAA Enzimas mejoradas y detergentes que las contienen.
USH1468H (en) 1994-04-28 1995-08-01 Costa Jill B Detergent compositions containing cellulase enzyme and selected perfumes for improved odor and stability
US6599730B1 (en) * 1994-05-02 2003-07-29 Procter & Gamble Company Subtilisin 309 variants having decreased adsorption and increased hydrolysis
EP0693549A1 (de) 1994-07-19 1996-01-24 The Procter & Gamble Company Feste Bleichaktivatorzusammensetzungen
CA2197203A1 (en) * 1994-08-11 1996-02-22 Genencor International, Inc. An improved cleaning composition
GB9416841D0 (en) 1994-08-19 1994-10-12 Finnfeeds Int Ltd An enzyme feed additive and animal feed including it
US5780285A (en) * 1995-03-03 1998-07-14 Genentech, Inc. Subtilisin variants capable of cleaving substrates containing dibasic residues
US5837516A (en) * 1995-03-03 1998-11-17 Genentech, Inc. Subtilisin variants capable of cleaving substrates containing basic residues
US6455295B1 (en) * 1995-03-08 2002-09-24 The Procter & Gamble Company Subtilisin Carlsberg variants having decreased adsorption and increased hydrolysis
US6475765B1 (en) * 1995-03-09 2002-11-05 Procter & Gamble Company Subtilisin DY variants having decreased adsorption and increased hydrolysis
IL117350A0 (en) * 1995-03-09 1996-07-23 Procter & Gamble Proteinase k variants having decreased adsorption and increased hydrolysis
US6682924B1 (en) * 1995-05-05 2004-01-27 Novozymes A/S Protease variants and compositions
DE69502097T2 (de) 1995-07-13 1998-11-12 Procter & Gamble Verpackte schäumende Zusammensetzung
EP0753567A1 (de) 1995-07-14 1997-01-15 The Procter & Gamble Company Beim Waschen weichmachende Zusammensetzungen
DE19530816A1 (de) 1995-08-23 1997-02-27 Cognis Bio Umwelt Verwendung von mutierter Subtilisin-Protease in kosmetischen Produkten
EP0778342A1 (de) 1995-12-06 1997-06-11 The Procter & Gamble Company Waschmittelzusammensetzungen
US5858948A (en) * 1996-05-03 1999-01-12 Procter & Gamble Company Liquid laundry detergent compositions comprising cotton soil release polymers and protease enzymes
WO1997042282A1 (en) 1996-05-03 1997-11-13 The Procter & Gamble Company Detergent compositions comprising polyamine polymers with improved soil dispersancy
US5958739A (en) * 1996-06-06 1999-09-28 Genencor International Inc. Mutant α-amylase
CN1113088C (zh) * 1996-09-24 2003-07-02 普罗格特-甘布尔公司 含有蛋白酶和蛋白酶抑制剂的液体洗涤剂
CA2266525A1 (en) 1996-09-24 1998-04-02 Charles Winston Saunders Liquid laundry detergent compositions containing proteolytic enzyme and protease inhibitors
US6165966A (en) * 1996-09-24 2000-12-26 The Procter & Gamble Company Liquid detergents containing proteolytic enzyme and protease inhibitors
DE69722591T2 (de) * 1996-10-29 2004-05-06 Koninklijke Philips Electronics N.V. Fernsehempfänger
EP0948610B1 (de) 1996-11-04 2011-05-25 Novozymes A/S Subtilase-varianten und verbindungen
BR9712878A (pt) 1996-11-04 2000-02-01 Novo Nordisk As Variante de enzima subtilase, processos para a identificação de uma variante de protease apresentando estabilidade autoproteolìtica e paraq a produção de uma enzima subtilase mutante e de uma variante de subtilase, sequência de dna, vetor, célula hospedeira microbiana, composição e uso de uma variante de subtilase.
EP0958346A1 (de) * 1996-12-31 1999-11-24 The Procter & Gamble Company Waschmittelzusammensetzungen entnthaltend mittel zum befestigen von farbstoffen
DK0958342T3 (da) 1996-12-31 2003-10-27 Procter & Gamble Fortykkede stærkt vandige væskeformige detergentsammensætninger
CA2276578A1 (en) * 1996-12-31 1998-07-09 Sherri L. Randall Laundry detergent compositions with polyamide-polyamines
EP0958341A1 (de) 1996-12-31 1999-11-24 The Procter & Gamble Company Verdickte, hochwässrige, kostengünstige, flüssige reinigungszusammensetzungen mit aromatische tenside
DE19717346C2 (de) * 1997-04-24 1999-05-20 Max Planck Gesellschaft DNA-Sonden, Verfahren und Kit zur Identifizierung Antibiotika-resistenter Bakterienstämme
US6080568A (en) * 1997-08-19 2000-06-27 Genencor International, Inc. Mutant α-amylase comprising modification at residues corresponding to A210, H405 and/or T412 in Bacillus licheniformis
JP2001514846A (ja) 1997-08-29 2001-09-18 ノボ ノルディスク アクティーゼルスカブ プロテアーゼ変異体及び組成物
JP5095884B2 (ja) 1997-08-29 2012-12-12 ノボザイムス アクティーゼルスカブ プロテアーゼ変異体及び組成物
US6773907B2 (en) 1997-11-21 2004-08-10 Peter Kamp Hansen Subtilase enzymes
CA2310454C (en) 1997-11-21 2012-01-24 Novo Nordisk A/S Protease variants and compositions
US6780629B2 (en) 1997-11-21 2004-08-24 Novozymes A/S Subtilase enzymes
WO1999031959A2 (en) 1997-12-20 1999-07-01 Genencor International, Inc. Accelerated stability test for granulated protein
WO1999032612A1 (en) 1997-12-20 1999-07-01 Genencor International, Inc. Fluidized bed matrix granule
CN1197946C (zh) 1997-12-20 2005-04-20 金克克国际有限公司 带有水合屏障材料的颗粒
CZ302332B6 (cs) 1997-12-20 2011-03-16 Genencor International, Inc. Vrstvená granule s jedinou základní cásticí
US6908757B1 (en) 1998-03-26 2005-06-21 The Procter & Gamble Company Serine protease variants having amino acid deletions and substitutions
AU1240900A (en) 1998-10-27 2000-05-15 Genencor International, Inc. Matrix granule
ATE494355T1 (de) 1998-11-13 2011-01-15 Danisco Us Inc Im wirbelschichtbett hergestelltes granulat mit niedrigem schüttgewicht
EP1803817B1 (de) 1998-12-18 2011-04-06 Novozymes A/S Subtilase Enzyme der I-S1- und I-S2-Untergruppen mit einem zusätzlichen Aminosäurerest in einer aktiven Schleifenregion
DK1149151T3 (da) 1999-01-08 2006-07-31 Genencor Int Sammensætninger med lav densitet og partikulære dele, der indbefatter dem
ATE402996T1 (de) 1999-05-20 2008-08-15 Novozymes As Subtilase-enzyme der i-s1 und i-s2 untergruppen mit wenigstens einem zusätzlichen aminosäurerest zwischen positionen 125 und 126
EP1183341B2 (de) 1999-05-20 2012-05-02 Novozymes A/S Subtilase enzyme der i-s1 und i-s2 untergruppen mit mindestens einer zusätzlichen aminosäure zwischen position 127 und 128
ATE408678T1 (de) 1999-05-20 2008-10-15 Novozymes As Subtilase enzyme der i-s1 und i-s2 untergruppen mit mindestens einer zusätzlichen aminosäure zwischen position 129 und 130
AU4392500A (en) 1999-05-20 2000-12-12 Novozymes A/S Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additionalamino acid residue between positions 132 and 133
AU4392800A (en) 1999-05-20 2000-12-12 Novozymes A/S Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additionalamino acid residue between positions 126 and 127
EP1183342B2 (de) 1999-05-20 2012-06-13 Novozymes A/S Subtilase enzyme der i-s1 und i-s2 untergruppen mit mindestens einer zusätzlichen aminosäure zwischen position 128 und 129
MXPA02000840A (es) 1999-07-22 2002-07-30 Procter & Gamble Variantes de proteasa de subtilisina que tienen substituciones de aminoacidos en regiones de epitopes definidas.
US6946128B1 (en) 1999-07-22 2005-09-20 The Procter & Gamble Company Protease conjugates having sterically protected epitope regions
EP1196548A2 (de) 1999-07-22 2002-04-17 The Procter & Gamble Company Proteasekonjugaten mit sterisch geschützten hydrolysierbaren stellen
JP2003505067A (ja) 1999-07-22 2003-02-12 ザ、プロクター、エンド、ギャンブル、カンパニー 特定のエピトープ領域にアミノ酸欠失及び置換を有するサブチリシンプロテアーゼの変異体
US6558939B1 (en) 1999-08-31 2003-05-06 Novozymes, A/S Proteases and variants thereof
AU6820000A (en) 1999-08-31 2001-03-26 Novozymes A/S Novel proteases and variants thereof
US7217554B2 (en) 1999-08-31 2007-05-15 Novozymes A/S Proteases and variants thereof
AU5911599A (en) * 1999-09-09 2001-04-10 Procter & Gamble Company, The A detergent composition containing a protease
BR9917498A (pt) 1999-09-22 2002-05-21 Procter & Gamble Recipiente de lìquidos para ser seguro com a mão
ES2348461T3 (es) 1999-10-15 2010-12-07 Danisco Us Inc. Gránulos que contienen proteínas y formulaciones de gránulos.
US6727085B2 (en) 1999-12-15 2004-04-27 Fanoe Tina Sejersgaard Subtilase variants having an improved wash performance on egg stains
US6855548B2 (en) 2000-02-08 2005-02-15 F. Hoffman-La Roche Ag Use of acid-stable proteases in animal feed
US6960462B2 (en) 2000-02-08 2005-11-01 Dsm Ip Assets B.V Use of acid-stable subtilisin proteases in animal feed
US6777218B1 (en) 2000-03-14 2004-08-17 Novozymes A/S Subtilase enzymes having an improved wash performance on egg stains
US6872696B2 (en) 2000-10-27 2005-03-29 Genencor International, Inc. Particle with substituted polyvinyl alcohol coating
US6803222B2 (en) 2000-11-22 2004-10-12 Kao Corporation Alkaline proteases
WO2002077187A2 (en) 2001-03-23 2002-10-03 Genencor International, Inc. Proteins producing an altered immunogenic response and methods of making and using the same
US8076113B2 (en) * 2001-04-02 2011-12-13 Danisco Us Inc. Method for producing granules with reduced dust potential comprising an antifoam agent
US7157416B2 (en) 2001-07-20 2007-01-02 Genencor International, Inc. Stabilization of enzymes
US7153820B2 (en) 2001-08-13 2006-12-26 Ecolab Inc. Solid detergent composition and method for solidifying a detergent composition
DE10153792A1 (de) 2001-10-31 2003-05-22 Henkel Kgaa Neue Alkalische Protease-Varianten und Wasch- und Reinigungsmittel enthaltend diese neuen Alkalischen Protease-Varianten
DE10162728A1 (de) 2001-12-20 2003-07-10 Henkel Kgaa Neue Alkalische Protease aus Bacillus gibsonii (DSM 14393) und Wasch-und Reinigungsmittel enthaltend diese neue Alkalische Protease
DE10162727A1 (de) 2001-12-20 2003-07-10 Henkel Kgaa Neue Alkalische Protease aus Bacillus gibsonii (DSM 14391) und Wasch-und Reinigungsmittel enthaltend diese neue Alkalische Protease
DE10163884A1 (de) 2001-12-22 2003-07-10 Henkel Kgaa Neue Alkalische Protease aus Bacillus sp. (DSM 14392) und Wasch- und Reinigungsmittel enthaltend diese neue Alkalische Protease
JP2005535284A (ja) 2001-12-31 2005-11-24 ジェネンコー・インターナショナル・インク 免疫反応の変化を生じるプロテアーゼ、ならびにその製造および利用方法
WO2003057246A1 (en) * 2001-12-31 2003-07-17 Genencor International, Inc. Proteases producing an altered immunological response and methods of making and using the same
US8124399B2 (en) 2002-03-29 2012-02-28 Danisco Us Inc. Enhanced protein expression in Bacillus
EP2431048B1 (de) 2002-10-08 2015-03-11 Danisco US Inc. Phenolbindepeptide
US7888093B2 (en) * 2002-11-06 2011-02-15 Novozymes A/S Subtilase variants
ES2291883T3 (es) 2003-02-07 2008-03-01 Novozymes A/S Proteasas.
BRPI0409992A (pt) 2003-05-07 2006-05-09 Novozymes As enzima subtilase, polinucleotìdeo isolado, construção de ácido nucleico, vetor de expressão recombinante, célula hospedeira recombinante, método para produzir a subtilase, composição limpadora ou detergente, usos de uma subtilase e de uma composição limpadora ou detergente, e, métodos para limpar ou lavar louças, lavar uma superfìcie dura ou lavar roupas e para remover manchas de ovo de uma superfìcie dura ou de roupas para lavar
BRPI0414959A (pt) 2003-10-10 2006-11-07 Novozymes As variante de uma protease precursora, sequência de ácido nucleico isolado, construção de ácido nucleico, vetor de expressão e célula hospedeira recombinantes, métodos para produzir a variante de protease, para melhorar o valor nutricional de uma ração animal, e para tratar proteìnas, planta transgênica, ou parte da planta, animal transgênico, não humano, ou produtos, ou elementos deste, aditivo e composição de ração animal, uso da variante de protease e/ou da composição, protease, e, estrutura 3d
EP1678296B1 (de) 2003-10-23 2011-07-13 Novozymes A/S Protease mit verbesserter stabilität in detergentien
ATE454440T1 (de) 2004-05-17 2010-01-15 Procter & Gamble Bleichmittel mit carbohydratoxidase
EP1766001B1 (de) 2004-06-21 2012-01-11 Novozymes A/S Nocardiopsis-proteasen
JP4681283B2 (ja) 2004-11-18 2011-05-11 独立行政法人海洋研究開発機構 新規な高アルカリプロテアーゼ及びその利用
JP4485341B2 (ja) 2004-12-20 2010-06-23 花王株式会社 組換え微生物
EP1700907A1 (de) 2005-03-11 2006-09-13 Unilever N.V. Flüssige bleichmittelzusammensetzung
EP1700904A1 (de) 2005-03-11 2006-09-13 Unilever N.V. Flüssige Waschmittelzusammensetzung
US7666963B2 (en) 2005-07-21 2010-02-23 Akzo Nobel N.V. Hybrid copolymers
US20100029538A1 (en) 2006-04-14 2010-02-04 Anna-Liisa Auterinen One-Step Treatment of Textiles
WO2007122175A1 (en) 2006-04-20 2007-11-01 Novozymes A/S Savinase variants having an improved wash performance on egg stains
US20080020961A1 (en) 2006-07-21 2008-01-24 Rodrigues Klin A Low Molecular Weight Graft Copolymers
NO20073834L (no) 2006-07-21 2008-01-22 Akzo Nobel Chemicals Int Bv Sulfonerte podede kopolymerer
US8093200B2 (en) 2007-02-15 2012-01-10 Ecolab Usa Inc. Fast dissolving solid detergent
BRPI0810863A2 (pt) 2007-04-30 2014-10-07 Danisco Us Inc Genencor Div Uso de hidrolisados de proteína para estabilizar formulações de detergente de metaloprotease.
SG148934A1 (en) 2007-06-11 2009-01-29 Novozymes As A process for combined biopolishing and bleach clean-up
US8383589B2 (en) 2007-07-09 2013-02-26 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Aegyptin and uses thereof
KR101575741B1 (ko) 2007-10-18 2015-12-08 에코랍 인코퍼레이티드 압축된 왁시 고형 세정 조성물 및 이의 제조 방법
DK2205731T3 (da) * 2007-10-31 2013-03-11 Danisco Us Inc Anvendelse og fremstilling af citrat-stabile, neutrale metalloproteaser
NZ586750A (en) 2008-02-14 2012-01-12 Danisco Us Inc Small enzyme-containing granules
US8153574B2 (en) 2009-03-18 2012-04-10 The Procter & Gamble Company Structured fluid detergent compositions comprising dibenzylidene polyol acetal derivatives and detersive enzymes
US8293697B2 (en) 2009-03-18 2012-10-23 The Procter & Gamble Company Structured fluid detergent compositions comprising dibenzylidene sorbitol acetal derivatives
EP2435547A1 (de) 2009-05-26 2012-04-04 The Procter & Gamble Company Wässriges flüssigmittel zur vorbehandlung von verschmutztem geschirr
DE102009027045A1 (de) * 2009-06-19 2010-12-30 Henkel Ag & Co. Kgaa Neue Proteasen und Mittel enthaltend diese Proteasen
WO2011005623A1 (en) 2009-07-09 2011-01-13 The Procter & Gamble Company Laundry detergent composition comprising low level of bleach
US20110009307A1 (en) 2009-07-09 2011-01-13 Alan Thomas Brooker Laundry Detergent Composition Comprising Low Level of Sulphate
JP5645937B2 (ja) 2009-07-31 2014-12-24 アクゾ ノーベル ナムローゼ フェンノートシャップＡｋｚｏ Ｎｏｂｅｌ Ｎ．Ｖ． パーソナルケア用途のためのハイブリッドコポリマー組成物
JP5882991B2 (ja) 2010-05-14 2016-03-09 ザ サン プロダクツ コーポレーション ポリマー含有洗浄組成物ならびにその生成法および使用法
US20130224757A1 (en) 2010-08-19 2013-08-29 Novozymes A/S Induced sporulation screening method
BR112013028007A2 (pt) 2011-05-20 2017-01-10 Ecolab Usa Inc detergentes não-fosfato e ácidos não-fosfóricos em um sistema alternado alcalinidade/acidez para a lavagem de louça
CA2856820C (en) 2011-05-20 2019-10-29 Ecolab Usa Inc. Acid formulations for use in a system for warewashing
US8841246B2 (en) 2011-08-05 2014-09-23 Ecolab Usa Inc. Cleaning composition containing a polysaccharide hybrid polymer composition and methods of improving drainage
US8679366B2 (en) 2011-08-05 2014-03-25 Ecolab Usa Inc. Cleaning composition containing a polysaccharide graft polymer composition and methods of controlling hard water scale
US8853144B2 (en) 2011-08-05 2014-10-07 Ecolab Usa Inc. Cleaning composition containing a polysaccharide graft polymer composition and methods of improving drainage
US8636918B2 (en) 2011-08-05 2014-01-28 Ecolab Usa Inc. Cleaning composition containing a polysaccharide hybrid polymer composition and methods of controlling hard water scale
BR112014009040A2 (pt) 2011-11-04 2017-05-09 Akzo Nobel Chemicals Int Bv copolímero obtenível através da polimerização de pelo menos um primeiro monômero etilenicamente não saturado e pelo menos um segundo monômero etilenicamente não saturado; composição de copolímero; e processo para preparação do copolímero de dendrito
JP2014532791A (ja) 2011-11-04 2014-12-08 アクゾ ノーベル ケミカルズ インターナショナル ベスローテン フエンノートシャップＡｋｚｏ Ｎｏｂｅｌ Ｃｈｅｍｉｃａｌｓ Ｉｎｔｅｒｎａｔｉｏｎａｌ Ｂ．Ｖ． ハイブリッド樹状コポリマー、その組成物及びそれを製造する方法
CN103998590B (zh) 2011-12-13 2019-02-01 艺康美国股份有限公司 浓的器皿洗涤组合物和方法
JP6040987B2 (ja) * 2012-07-06 2016-12-07 東洋紡株式会社 改変型アルカリホスファターゼ
EP2877562B1 (de) 2012-07-26 2018-04-25 The Procter and Gamble Company Enzymhaltige flüssige reinigungszusammensetzungen mit niedrigem ph-wert
US8945314B2 (en) 2012-07-30 2015-02-03 Ecolab Usa Inc. Biodegradable stability binding agent for a solid detergent
US9745543B2 (en) 2012-09-10 2017-08-29 Ecolab Usa Inc. Stable liquid manual dishwashing compositions containing enzymes
CN105026541A (zh) 2012-10-04 2015-11-04 艺康美国股份有限公司 用于洗衣和其他硬表面清洁的预浸泡工艺
US9139458B2 (en) 2013-03-15 2015-09-22 Janet Angel Compositions and methods of use
US9796950B2 (en) 2013-09-09 2017-10-24 Ecolab Usa Inc. Synergistic stain removal through an alkali metal hydroxide-based detergent composition with novel chelator combination
CN105518118B (zh) 2013-09-09 2019-09-17 艺康美国股份有限公司 通过螯合剂组合来协同去污
JP6254693B2 (ja) 2013-10-24 2017-12-27 エコラボ ユーエスエー インコーポレイティド 表面から汚れを除去する組成物及び方法
US9365805B2 (en) 2014-05-15 2016-06-14 Ecolab Usa Inc. Bio-based pot and pan pre-soak
BR112017002158B1 (pt) 2014-08-01 2022-03-15 Ecolab Usa Inc Método de limpeza manual de superfície usando produtos têxteis de limpeza e sistema
US10577571B2 (en) 2016-11-08 2020-03-03 Ecolab Usa Inc. Non-aqueous cleaner for vegetable oil soils
WO2020099491A1 (en) 2018-11-14 2020-05-22 Novozymes A/S Oral care composition comprising a polypeptide having dnase activity
CN114207101B (zh) 2019-07-31 2024-05-31 埃科莱布美国股份有限公司 免用个人防护设备的脱灰剂组合物
US11873465B2 (en) 2019-08-14 2024-01-16 Ecolab Usa Inc. Methods of cleaning and soil release of highly oil absorbing substrates employing optimized extended chain nonionic surfactants
CN110760466B (zh) * 2019-11-18 2021-04-20 山东隆科特酶制剂有限公司 一株产耐高温中性蛋白酶的枯草芽孢杆菌及其应用
AU2021306707B2 (en) 2020-07-06 2024-06-13 Ecolab Usa Inc. Foaming mixed alcohol/water compositions comprising a structured alkoxylated siloxane
EP4176031A1 (de) 2020-07-06 2023-05-10 Ecolab USA Inc. Peg-modifizierte rizinusöl-basierte zusammensetzungen zur mikroemulgierung und entfernung von mehreren ölhaltigen böden
CN116075583A (zh) 2020-07-06 2023-05-05 埃科莱布美国股份有限公司 包含烷基硅氧烷和水溶助剂/增溶剂的组合的发泡混合醇/水组合物
EP4199897A2 (de) 2020-08-24 2023-06-28 Novozymes A/S Mundpflegezusammensetzung mit einer fructanase
US20240247209A1 (en) 2021-05-18 2024-07-25 Nouryon Chemicals International B.V. Polyester polyquats in cleaning applications
EP4341317A1 (de) 2021-05-20 2024-03-27 Nouryon Chemicals International B.V. Hergestellte polymere mit veränderter oligosaccharid- oder polysaccharid-funktionalität oder verringerter oligosaccharidverteilung, verfahren zu ihrer herstellung, zusammensetzungen damit und verfahren zu ihrer verwendung
US20240287409A1 (en) 2021-06-30 2024-08-29 Nouryon Chemicals International B.V. Chelate-amphoteric surfactant liquid concentrates and use thereof in cleaning applications
WO2023057367A1 (en) 2021-10-08 2023-04-13 Unilever Ip Holdings B.V. Laundry composition
WO2024020445A1 (en) 2022-07-20 2024-01-25 Ecolab Usa Inc. Novel nonionic extended surfactants, compositions and methods of use thereof

Family Cites Families (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US362957A (en) * 1887-05-17 haig-ht
US3557002A (en) * 1967-11-15 1971-01-19 Procter & Gamble Stabilized aqueous enzyme preparation
US3558498A (en) 1967-11-29 1971-01-26 Procter & Gamble Granular detergent composition containing enzymes and environmental control components
GB1240058A (en) 1968-04-12 1971-07-21 Procter & Gamble Enzyme-containing detergent compositions
US3623957A (en) 1970-01-21 1971-11-30 Baxter Laboratories Inc Preparation of microbial alkaline protease by fermentation with bacillus subtilis, variety licheniformis
BE785666A (fr) 1971-07-01 1973-01-02 Procter & Gamble Procede pour la production de compositions enzymatiques en granules
BE792982A (en) 1971-12-20 1973-06-19 Procter & Gamble Europ Proteolytic enzymes detergent - contg cationic and anionic surfactants
US4011169A (en) 1973-06-29 1977-03-08 The Procter & Gamble Company Stabilization and enhancement of enzymatic activity
DE2709476A1 (de) 1976-03-08 1977-09-15 Procter & Gamble Europ Fluessiges, enzymhaltiges wasch- und reinigungsmittel
US4090973A (en) 1976-06-24 1978-05-23 The Procter & Gamble Company Method for making stable detergent compositions
DE2633601A1 (de) 1976-07-27 1978-02-02 Henkel Kgaa Fluessiges, als wasch- und reinigungsmittel verwendbares, enzymhaltiges konzentrat
GB1603640A (en) 1977-07-20 1981-11-25 Gist Brocades Nv Enzyme particles
US4261868A (en) 1979-08-08 1981-04-14 Lever Brothers Company Stabilized enzymatic liquid detergent composition containing a polyalkanolamine and a boron compound
US4302544A (en) * 1979-10-15 1981-11-24 University Of Rochester Asporogenous mutant of B. subtilis for use as host component of HV1 system
US4318818A (en) 1979-11-09 1982-03-09 The Procter & Gamble Company Stabilized aqueous enzyme composition
JPS5950280B2 (ja) 1980-10-24 1984-12-07 花王株式会社 酵素入り漂白剤組成物
JPS5786299A (en) * 1980-11-19 1982-05-29 Dai Ichi Seiyaku Co Ltd Plasmid and its preparation
IE52434B1 (en) * 1981-01-15 1987-10-28 Cpc International Inc A process for cloning the gene coding for a thermostable alpha-amylase into escherichia coli and bacillus subtilis
US4404128A (en) 1981-05-29 1983-09-13 The Procter & Gamble Company Enzyme detergent composition
DE3122216A1 (de) * 1981-06-04 1982-12-23 Boehringer Mannheim Gmbh, 6800 Mannheim Verfahren zur herstellung eines mikroorganismus, welcher (alpha)-galactosidase, aber keine invertase bildet, so erhaltener mikroorganismus und seine verwendung
DE3264685D1 (en) 1981-11-13 1985-08-14 Unilever Nv Enzymatic liquid cleaning composition
JPS58162291A (ja) * 1982-03-23 1983-09-26 Res Dev Corp Of Japan 耐熱性プロテア−ゼの製造法
GR78180B (de) * 1982-04-15 1984-09-26 Genentech Inc
JPS5959190A (ja) * 1982-09-29 1984-04-04 Agency Of Ind Science & Technol プロテア−ゼの製造法
DK135983D0 (da) * 1983-03-25 1983-03-25 Novo Industri As Maltogen amylaseenzymprodukt og fremgangsmade til dets fremstilling og anvendelse
NZ208612A (en) 1983-06-24 1991-09-25 Genentech Inc Method of producing "procaryotic carbonyl hydrolases" containing predetermined, site specific mutations

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