ES2149244T3 - Procedimientos para producir proteina c. - Google Patents

Procedimientos para producir proteina c.

Info

Publication number
ES2149244T3
ES2149244T3 ES94309314T ES94309314T ES2149244T3 ES 2149244 T3 ES2149244 T3 ES 2149244T3 ES 94309314 T ES94309314 T ES 94309314T ES 94309314 T ES94309314 T ES 94309314T ES 2149244 T3 ES2149244 T3 ES 2149244T3
Authority
ES
Spain
Prior art keywords
high levels
procedures
cell lines
producing protein
cells
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES94309314T
Other languages
English (en)
Inventor
Brian William Grinnell
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eli Lilly and Co
Original Assignee
Eli Lilly and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eli Lilly and Co filed Critical Eli Lilly and Co
Application granted granted Critical
Publication of ES2149244T3 publication Critical patent/ES2149244T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/745—Blood coagulation or fibrinolysis factors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6464—Protein C (3.4.21.69)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2523/00—Culture process characterised by temperature

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Hematology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

LA PRESENTE INVENCION SE REFIERE A UN METODO PARA PRODUCIR ELEVADOS NIVELES DE PROTEINAS RECOMBINANTES FUNCIONALES EN CELULAS DE MAMIFEROS TRANSFORMADAS ADENOVIRUS, POR INCUBACION DE CELULAS CAPACES DE PRODUCIR PROTEINAS RECOMBINANTES EN UN MARGEN DE TEMPERATURA ENTRE APROXIMADAMENTE 38 (GRADOS) C Y APROXIMADAMENTE 39 (GRADOS) C. EL METODO TIENE EN CUENTA ELEVADOS NIVELES DE EXPRESION DE PROTEINA TOTAL EN ALGUNAS LINEAS DE CELULAS Y ELEVADOS NIVELES DE PROTEINA FUNCIONAL EN OTRAS LINEAS DE CELULAS.
ES94309314T 1993-12-15 1994-12-14 Procedimientos para producir proteina c. Expired - Lifetime ES2149244T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/168,035 US5618714A (en) 1993-12-15 1993-12-15 Methods for producing protein C

Publications (1)

Publication Number Publication Date
ES2149244T3 true ES2149244T3 (es) 2000-11-01

Family

ID=22609820

Family Applications (1)

Application Number Title Priority Date Filing Date
ES94309314T Expired - Lifetime ES2149244T3 (es) 1993-12-15 1994-12-14 Procedimientos para producir proteina c.

Country Status (28)

Country Link
US (1) US5618714A (es)
EP (1) EP0658626B1 (es)
JP (1) JP3662614B2 (es)
KR (1) KR950018454A (es)
CN (1) CN1107887A (es)
AT (1) ATE196165T1 (es)
AU (1) AU687914B2 (es)
BR (1) BR9404991A (es)
CA (1) CA2138101A1 (es)
CO (1) CO4340654A1 (es)
CZ (2) CZ315794A3 (es)
DE (1) DE69425809T2 (es)
DK (1) DK0658626T3 (es)
ES (1) ES2149244T3 (es)
FI (1) FI106385B (es)
GR (1) GR3034909T3 (es)
HU (1) HU219508B (es)
IL (1) IL111983A0 (es)
NO (1) NO944846L (es)
NZ (1) NZ270140A (es)
PE (1) PE42695A1 (es)
PL (1) PL176381B1 (es)
PT (1) PT658626E (es)
RU (1) RU2110574C1 (es)
SI (1) SI0658626T1 (es)
UA (1) UA26870C2 (es)
YU (1) YU73494A (es)
ZA (1) ZA949981B (es)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19724543A1 (de) * 1997-06-11 1998-12-17 Philips Patentverwaltung Wechsler-Gerät für Informationsplatten
WO2002069232A2 (en) 2001-02-19 2002-09-06 Merck Patent Gmbh Method for identification of t-cell epitopes and use for preparing molecules with reeduced immunogenicity
ATE386824T1 (de) 2002-03-29 2008-03-15 Merck & Co Inc Verfahren zur virusproduktion
US20070142272A1 (en) * 2003-01-24 2007-06-21 Zlokovic Berislav V Neuroprotective activity of activated protein c independent of its anticoagulant activity
JP2008507561A (ja) * 2004-07-23 2008-03-13 ザ ユニバーシティ オブ ロチェスター 活性化プロテインｃによる、脳内のプラスミノゲン活性化因子の不都合な作用の阻害
US20090148458A1 (en) * 2005-06-23 2009-06-11 The University Of British Columbia Coagulation factor iii polymorphisms associated with prediction of subject outcome and response to therapy
CA2654761A1 (en) * 2006-06-09 2007-12-13 The University Of British Columbia Interferon gamma polymorphisms as indicators of subject outcome in critically ill subjects
US20110171200A1 (en) * 2008-01-15 2011-07-14 Walley Keith R Protein c rs2069915 as a response predictor to survival and administration of activated protein c or protein c-like compound
WO2012068519A2 (en) 2010-11-19 2012-05-24 Sirius Genomics Inc. Markers associated with response to activated protein c administration, and uses thereof

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4775624A (en) * 1985-02-08 1988-10-04 Eli Lilly And Company Vectors and compounds for expression of human protein C
US4968626A (en) * 1985-08-15 1990-11-06 Board Of Reagents Of The University Of Washington DNA sequence coding for protein C
US4959318A (en) * 1985-06-27 1990-09-25 Zymogenetics, Inc. Expression of protein C
EP0245949B1 (en) * 1986-04-09 1997-10-29 Eli Lilly And Company A method of using eukaryotic expression vectors comprising the bk virus enhancer
AU8339187A (en) * 1986-11-17 1988-06-16 New England Medical Center Hospitals, Inc., The Enhancing gamma-carboxylation of recombinant vitamin k-dependent proteins
US4992373A (en) * 1987-12-04 1991-02-12 Eli Lilly And Company Vectors and compounds for direct expression of activated human protein C
ZA889497B (en) * 1987-12-28 1990-08-29 Lilly Co Eli Vectors and compounds for expression of zymogen forms of human protein c
JPH0246296A (ja) * 1988-08-09 1990-02-15 Hoechst Japan Ltd 雑種プロテインｃ及びその製造方法
US4981952A (en) * 1988-10-04 1991-01-01 Eli Lilly And Company Method for the purification of vitamin K-dependent proteins
CA1332049C (en) * 1988-10-07 1994-09-20 Eli Lilly And Company Eukaryotic expression
DK0485504T3 (da) * 1989-08-11 1994-04-18 Zymogenetics Inc Fremgangsmåde til fremstilling af aktiveret protein C ved celledyrkning
IL97312A (en) * 1990-02-23 1999-01-26 Lilly Co Eli A method of generating a polypeptide in an eukaryotic vector AND recombinant surrogate cell containing an enhanced transcriptional control unit based on the primary late adenovirus coefficient
AU1180692A (en) * 1991-01-29 1992-08-27 Teijin Limited Human protein c expression vector

Also Published As

Publication number Publication date
GR3034909T3 (en) 2001-02-28
CN1107887A (zh) 1995-09-06
RU94043769A (ru) 1997-03-27
JPH07222589A (ja) 1995-08-22
DE69425809T2 (de) 2001-02-01
ZA949981B (en) 1996-06-14
PT658626E (pt) 2001-01-31
HU9403600D0 (en) 1995-02-28
KR950018454A (ko) 1995-07-22
CA2138101A1 (en) 1995-06-16
HU219508B (hu) 2001-04-28
FI106385B (fi) 2001-01-31
FI945877L (fi) 1995-06-16
FI945877A0 (fi) 1994-12-14
NZ270140A (en) 1996-05-28
PE42695A1 (es) 1995-12-12
PL306291A1 (en) 1995-06-26
ATE196165T1 (de) 2000-09-15
NO944846D0 (no) 1994-12-14
RU2110574C1 (ru) 1998-05-10
AU8045294A (en) 1995-06-22
CZ315794A3 (en) 1995-10-18
JP3662614B2 (ja) 2005-06-22
CO4340654A1 (es) 1996-07-30
US5618714A (en) 1997-04-08
NO944846L (no) 1995-06-16
EP0658626B1 (en) 2000-09-06
CZ290724B6 (cs) 2002-10-16
PL176381B1 (pl) 1999-05-31
IL111983A0 (en) 1995-03-15
BR9404991A (pt) 1995-08-08
EP0658626A1 (en) 1995-06-21
UA26870C2 (uk) 1999-12-29
DE69425809D1 (de) 2000-10-12
HUT69979A (en) 1995-09-28
SI0658626T1 (en) 2001-02-28
YU73494A (sh) 1997-03-07
DK0658626T3 (da) 2000-10-16
AU687914B2 (en) 1998-03-05

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