NO944846L - Fremgangsmåte for fremstilling av protein C - Google Patents

Fremgangsmåte for fremstilling av protein C

Info

Publication number
NO944846L
NO944846L NO944846A NO944846A NO944846L NO 944846 L NO944846 L NO 944846L NO 944846 A NO944846 A NO 944846A NO 944846 A NO944846 A NO 944846A NO 944846 L NO944846 L NO 944846L
Authority
NO
Norway
Prior art keywords
protein
cell lines
recombinant proteins
levels
preparation
Prior art date
Application number
NO944846A
Other languages
English (en)
Other versions
NO944846D0 (no
Inventor
Brian William Grinnell
Original Assignee
Lilly Co Eli
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lilly Co Eli filed Critical Lilly Co Eli
Publication of NO944846D0 publication Critical patent/NO944846D0/no
Publication of NO944846L publication Critical patent/NO944846L/no

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/745—Blood coagulation or fibrinolysis factors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6464—Protein C (3.4.21.69)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2523/00—Culture process characterised by temperature

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Hematology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

Det blir beskrevet en fremgangsmåte for produsering av høye nivåer av funskjonelt rekomblnante proteiner l adenovlrus-transformerte mammalske celler ved å Inkubere celler som er Istand til å produsere rekombl- nante proteiner ved en temperatur l området mellom omkring 38*C og omkring 39'C. Fremgangsmåten muliggjør høyere ekstpresjonsnlvåer av totalt protein l noen cellelinjer og høyere nivåer av funksjonelt protein l andre cellelinjer.
NO944846A 1993-12-15 1994-12-14 Fremgangsmåte for fremstilling av protein C NO944846L (no)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/168,035 US5618714A (en) 1993-12-15 1993-12-15 Methods for producing protein C

Publications (2)

Publication Number Publication Date
NO944846D0 NO944846D0 (no) 1994-12-14
NO944846L true NO944846L (no) 1995-06-16

Family

ID=22609820

Family Applications (1)

Application Number Title Priority Date Filing Date
NO944846A NO944846L (no) 1993-12-15 1994-12-14 Fremgangsmåte for fremstilling av protein C

Country Status (28)

Country Link
US (1) US5618714A (no)
EP (1) EP0658626B1 (no)
JP (1) JP3662614B2 (no)
KR (1) KR950018454A (no)
CN (1) CN1107887A (no)
AT (1) ATE196165T1 (no)
AU (1) AU687914B2 (no)
BR (1) BR9404991A (no)
CA (1) CA2138101A1 (no)
CO (1) CO4340654A1 (no)
CZ (2) CZ315794A3 (no)
DE (1) DE69425809T2 (no)
DK (1) DK0658626T3 (no)
ES (1) ES2149244T3 (no)
FI (1) FI106385B (no)
GR (1) GR3034909T3 (no)
HU (1) HU219508B (no)
IL (1) IL111983A0 (no)
NO (1) NO944846L (no)
NZ (1) NZ270140A (no)
PE (1) PE42695A1 (no)
PL (1) PL176381B1 (no)
PT (1) PT658626E (no)
RU (1) RU2110574C1 (no)
SI (1) SI0658626T1 (no)
UA (1) UA26870C2 (no)
YU (1) YU73494A (no)
ZA (1) ZA949981B (no)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19724543A1 (de) * 1997-06-11 1998-12-17 Philips Patentverwaltung Wechsler-Gerät für Informationsplatten
MXPA03007316A (es) 2001-02-19 2003-12-04 Merck Patent Gmbh Metodo para la identificacion de epitopes de celulas t y el uso para la preparacion de moleculas con inmunogenicidad reducida.
EP1492891B1 (en) * 2002-03-29 2008-02-20 Merck & Co., Inc. Methods of virus production
US20070142272A1 (en) * 2003-01-24 2007-06-21 Zlokovic Berislav V Neuroprotective activity of activated protein c independent of its anticoagulant activity
JP2008507561A (ja) * 2004-07-23 2008-03-13 ザ ユニバーシティ オブ ロチェスター 活性化プロテインｃによる、脳内のプラスミノゲン活性化因子の不都合な作用の阻害
AU2006261555A1 (en) * 2005-06-23 2006-12-28 The University Of British Columbia Coagulation factor lll polymorphisms associated with prediction of subject outcome and response to therapy
CA2654761A1 (en) * 2006-06-09 2007-12-13 The University Of British Columbia Interferon gamma polymorphisms as indicators of subject outcome in critically ill subjects
US20110171200A1 (en) * 2008-01-15 2011-07-14 Walley Keith R Protein c rs2069915 as a response predictor to survival and administration of activated protein c or protein c-like compound
WO2012068519A2 (en) 2010-11-19 2012-05-24 Sirius Genomics Inc. Markers associated with response to activated protein c administration, and uses thereof

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4775624A (en) * 1985-02-08 1988-10-04 Eli Lilly And Company Vectors and compounds for expression of human protein C
US4968626A (en) * 1985-08-15 1990-11-06 Board Of Reagents Of The University Of Washington DNA sequence coding for protein C
US4959318A (en) * 1985-06-27 1990-09-25 Zymogenetics, Inc. Expression of protein C
EP0245949B1 (en) * 1986-04-09 1997-10-29 Eli Lilly And Company A method of using eukaryotic expression vectors comprising the bk virus enhancer
WO1988003926A1 (en) * 1986-11-17 1988-06-02 New England Medical Center Enhancing gamma-carboxylation of recombinant vitamin k-dependent proteins
US4992373A (en) * 1987-12-04 1991-02-12 Eli Lilly And Company Vectors and compounds for direct expression of activated human protein C
ZA889497B (en) * 1987-12-28 1990-08-29 Lilly Co Eli Vectors and compounds for expression of zymogen forms of human protein c
JPH0246296A (ja) * 1988-08-09 1990-02-15 Hoechst Japan Ltd 雑種プロテインｃ及びその製造方法
US4981952A (en) * 1988-10-04 1991-01-01 Eli Lilly And Company Method for the purification of vitamin K-dependent proteins
CA1332049C (en) * 1988-10-07 1994-09-20 Eli Lilly And Company Eukaryotic expression
JP3045307B2 (ja) * 1989-08-11 2000-05-29 ザイモジェネティクス，インコーポレイティド 活性化プロテインｃを生成するための細胞培養法
IL97312A (en) * 1990-02-23 1999-01-26 Lilly Co Eli A method of generating a polypeptide in an eukaryotic vector AND recombinant surrogate cell containing an enhanced transcriptional control unit based on the primary late adenovirus coefficient
JPH05276952A (ja) * 1991-01-29 1993-10-26 Teijin Ltd ヒトプロテインｃ発現ベクター

Also Published As

Publication number Publication date
AU8045294A (en) 1995-06-22
US5618714A (en) 1997-04-08
SI0658626T1 (en) 2001-02-28
JPH07222589A (ja) 1995-08-22
CA2138101A1 (en) 1995-06-16
ES2149244T3 (es) 2000-11-01
HUT69979A (en) 1995-09-28
KR950018454A (ko) 1995-07-22
ATE196165T1 (de) 2000-09-15
GR3034909T3 (en) 2001-02-28
IL111983A0 (en) 1995-03-15
PT658626E (pt) 2001-01-31
RU94043769A (ru) 1997-03-27
CZ315794A3 (en) 1995-10-18
HU9403600D0 (en) 1995-02-28
FI945877L (fi) 1995-06-16
JP3662614B2 (ja) 2005-06-22
DE69425809T2 (de) 2001-02-01
YU73494A (sh) 1997-03-07
RU2110574C1 (ru) 1998-05-10
NZ270140A (en) 1996-05-28
NO944846D0 (no) 1994-12-14
AU687914B2 (en) 1998-03-05
ZA949981B (en) 1996-06-14
CN1107887A (zh) 1995-09-06
FI106385B (fi) 2001-01-31
PL176381B1 (pl) 1999-05-31
FI945877A0 (fi) 1994-12-14
CO4340654A1 (es) 1996-07-30
DE69425809D1 (de) 2000-10-12
CZ290724B6 (cs) 2002-10-16
EP0658626A1 (en) 1995-06-21
PE42695A1 (es) 1995-12-12
BR9404991A (pt) 1995-08-08
DK0658626T3 (da) 2000-10-16
UA26870C2 (uk) 1999-12-29
EP0658626B1 (en) 2000-09-06
PL306291A1 (en) 1995-06-26
HU219508B (hu) 2001-04-28

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