ES2192060T3 - Analisis de bioluminiscencia para agonistas o antagonistas de un receptor acoplado al calcio. - Google Patents

Analisis de bioluminiscencia para agonistas o antagonistas de un receptor acoplado al calcio.

Info

Publication number
ES2192060T3
ES2192060T3 ES99930956T ES99930956T ES2192060T3 ES 2192060 T3 ES2192060 T3 ES 2192060T3 ES 99930956 T ES99930956 T ES 99930956T ES 99930956 T ES99930956 T ES 99930956T ES 2192060 T3 ES2192060 T3 ES 2192060T3
Authority
ES
Spain
Prior art keywords
calcium
bioluminiscence
agonists
antagonists
analysis
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES99930956T
Other languages
English (en)
Other versions
ES2192060T5 (es
Inventor
Vincent Dupriez
Michel Detheux
Marc Parmentier
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ogeda SA
Original Assignee
Euroscreen SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=8237065&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ES2192060(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Euroscreen SA filed Critical Euroscreen SA
Application granted granted Critical
Publication of ES2192060T3 publication Critical patent/ES2192060T3/es
Publication of ES2192060T5 publication Critical patent/ES2192060T5/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6872—Intracellular protein regulatory factors and their receptors, e.g. including ion channels
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Toxicology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

Método diagnóstico y/o dosificación de un agonista y/o antagonista del receptor acoplado al calcio o un canal acoplado al calcio o cualquier otra proteína acoplada al calcio, que consta de los siguientes pasos: disposición de un agonista y/o antagonista en un soporte sólido, incubación de una o más célula(s) que expresan la apoaecuorina o cualquier proteína relacionada y el denominado receptor acoplado al calcio con coelenteracina o cualquier otro cofactor de una proteína sensible al calcio con el fin de reconstituir una aecuorina activa por la(s) denominadas(s) célula(s), - adición de una o más de las denominadas células al denominado soporte sólido y - medición de la luz emitida por la(s) denominada(s) célula(s).
ES99930956T 1998-07-06 1999-07-06 Ensayo de bioluminiscencia para agonistas o antagonistas de un receptor acoplado al calcio. Expired - Lifetime ES2192060T5 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP98870149 1998-07-06
EP98870149 1998-07-06

Publications (2)

Publication Number Publication Date
ES2192060T3 true ES2192060T3 (es) 2003-09-16
ES2192060T5 ES2192060T5 (es) 2009-05-01

Family

ID=8237065

Family Applications (1)

Application Number Title Priority Date Filing Date
ES99930956T Expired - Lifetime ES2192060T5 (es) 1998-07-06 1999-07-06 Ensayo de bioluminiscencia para agonistas o antagonistas de un receptor acoplado al calcio.

Country Status (9)

Country Link
US (1) US6872538B1 (es)
EP (1) EP1145002B2 (es)
JP (1) JP3495987B2 (es)
AT (1) ATE232298T1 (es)
CA (1) CA2336929C (es)
DE (1) DE69905291T3 (es)
DK (1) DK1145002T4 (es)
ES (1) ES2192060T5 (es)
WO (1) WO2000002045A2 (es)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6025154A (en) 1995-06-06 2000-02-15 Human Genome Sciences, Inc. Polynucleotides encoding human G-protein chemokine receptor HDGNR10
US6248542B1 (en) 1997-12-09 2001-06-19 Massachusetts Institute Of Technology Optoelectronic sensor
DE10008373C2 (de) * 2000-02-23 2002-11-28 Helmut Adelsberger Verfahren und Vorrichtung zur Bestimmung der Ionenkanalaktivität
US7422860B2 (en) * 2001-02-07 2008-09-09 Massachusetts Institute Of Technology Optoelectronic detection system
US7214346B2 (en) 2001-02-07 2007-05-08 Massachusetts Institute Of Technology Optoelectronic detection system
US8216797B2 (en) * 2001-02-07 2012-07-10 Massachusetts Institute Of Technology Pathogen detection biosensor
US7175988B2 (en) 2001-02-09 2007-02-13 Human Genome Sciences, Inc. Human G-protein Chemokine Receptor (CCR5) HDGNR10
WO2002075273A2 (en) * 2001-03-20 2002-09-26 Euroscreen S.A. Screening, diagnostic and/or dosage method and device of an agonist and/or antagonist for a calcium-coupled receptor
GB0108143D0 (en) * 2001-03-31 2001-05-23 Univ Dundee High-throughput screen
US7393934B2 (en) 2001-12-21 2008-07-01 Human Genome Sciences, Inc. Human G-protein chemokine receptor (CCR5) HDGNR10
JP4360915B2 (ja) 2002-01-07 2009-11-11 ユーロスクリーン・ソシエテ・アノニム Ｇタンパク質共役受容体ｇｐｒ４３のリガンドおよびその使用
EP1900753B1 (en) 2002-11-08 2017-08-09 Ablynx N.V. Method of administering therapeutic polypeptides, and polypeptides therefor
GB0228139D0 (en) * 2002-12-03 2003-01-08 Kozlova Zwinderman Olga Fungal biosensor for contaminant detection
JP2006517789A (ja) 2003-01-10 2006-08-03 アブリンクス エン．ヴェー． 治療用ポリペプチド、その相同物、その断片、および血小板媒介凝集の調節での使用
ATE485307T1 (de) 2003-11-07 2010-11-15 Ablynx Nv Camelidae schwere ketten antikörper vhhs gegen epidermalen wachstumfaktor rezeptor (egfr) und ihre verwendung
EP1723178A4 (en) 2004-03-12 2007-12-12 Human Genome Sciences Inc HUMAN G-PROTEIN CHEMOKIN RECEPTOR (CCR5) HDGNR10
ITMI20040598A1 (it) * 2004-03-26 2004-06-26 Carlotta Giorgi Metodo per la rilevazione di parametri intracellulari con sonde proteiche luminescenti per lo screening di molecole in grado di alterare detti parametri
CA2504920C (en) 2004-05-14 2014-04-01 Euroscreen S.A. Ligand for g-protein coupled receptor fprl2 and uses thereof
US8188223B2 (en) 2005-05-18 2012-05-29 Ablynx N.V. Serum albumin binding proteins
ES2694247T3 (es) 2005-05-20 2018-12-19 Ablynx N.V. NanobodiesTM mejorados para el tratamiento de trastornos mediados por agregación
WO2007100618A2 (en) * 2006-02-23 2007-09-07 Atto Bioscience Novel cell-based phosphodiesterase assays
US20080287371A1 (en) * 2007-05-17 2008-11-20 Tranzyme Pharma Inc. Macrocyclic antagonists of the motilin receptor for modulation of the migrating motor complex
JP2012533298A (ja) * 2009-07-14 2012-12-27 イー・エム・デイー・ミリポア・コーポレイシヨン カルシウム親和性の増加と生物発光の増強を示す改変型発光蛋白質及びその使用

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5422266A (en) 1984-12-31 1995-06-06 University Of Georgia Research Foundation, Inc. Recombinant DNA vectors capable of expressing apoaequorin
JPH01285196A (ja) 1988-05-11 1989-11-16 Chisso Corp 発光蛋白エクオリンの製造法
JPH02253162A (ja) † 1989-03-27 1990-10-11 Chisso Corp 特異的結合能を有する物質と結合したエクオリンを用いる検出法
DE68922971T2 (de) † 1988-12-06 1995-10-19 Chisso Corp Mit einem Protein mit spezifischer Bindungsaktivität fusioniertes Aequorin, seine Herstellung, Reinigung und Nachweismethode.
JPH03153700A (ja) † 1989-11-09 1991-07-01 Chisso Corp 物理吸着法により担体に固定化されたエクオリン活性を示す蛋白質
US5714666A (en) † 1993-02-09 1998-02-03 Children's Hospital Of Philadelphia Measurement of intracellular calcium using bioluminescent apoaequorin expressed in mammalian cells
US5744320A (en) † 1995-06-07 1998-04-28 Promega Corporation Quenching reagents and assays for enzyme-mediated luminescence

Also Published As

Publication number Publication date
WO2000002045A2 (en) 2000-01-13
DE69905291T3 (de) 2009-06-25
EP1145002B1 (en) 2003-02-05
CA2336929C (en) 2005-10-04
DK1145002T4 (da) 2009-03-16
US6872538B1 (en) 2005-03-29
DE69905291T2 (de) 2003-11-06
ES2192060T5 (es) 2009-05-01
EP1145002A2 (en) 2001-10-17
DE69905291D1 (de) 2003-03-13
CA2336929A1 (en) 2000-01-13
EP1145002B2 (en) 2008-12-10
ATE232298T1 (de) 2003-02-15
JP2002519699A (ja) 2002-07-02
DK1145002T3 (da) 2003-05-05
WO2000002045A3 (en) 2001-11-08
JP3495987B2 (ja) 2004-02-09

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