ES2496596T3 - Método para producir trombomodulina soluble de gran pureza - Google Patents

Método para producir trombomodulina soluble de gran pureza Download PDF

Info

Publication number
ES2496596T3
ES2496596T3 ES08722575.1T ES08722575T ES2496596T3 ES 2496596 T3 ES2496596 T3 ES 2496596T3 ES 08722575 T ES08722575 T ES 08722575T ES 2496596 T3 ES2496596 T3 ES 2496596T3
Authority
ES
Spain
Prior art keywords
soluble thrombomodulin
thrombomodulin
denatured product
product
soluble
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
ES08722575.1T
Other languages
English (en)
Inventor
Susumu Ohigashi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Asahi Kasei Pharma Corp
Original Assignee
Asahi Kasei Pharma Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Asahi Kasei Pharma Corp filed Critical Asahi Kasei Pharma Corp
Application granted granted Critical
Publication of ES2496596T3 publication Critical patent/ES2496596T3/es
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/165—Extraction; Separation; Purification by chromatography mixed-mode chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/745—Blood coagulation or fibrinolysis factors
    • C07K14/7455—Thrombomodulin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Hematology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Toxicology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Diabetes (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Epidemiology (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Un método para producir trombomodulina soluble que no contiene sustancialmente un producto desnaturalizado de la trombomodulina soluble, que puede ser generado a partir de la trombomodulina soluble en condiciones ácidas, en donde el contenido del producto desnaturalizado de la trombomodulina soluble en la trombomodulina soluble que no contiene sustancialmente el producto desnaturalizado de trombomodulina soluble es 3 % o menos, que comprende: (0) una etapa en la que se deja la trombomodulina soluble en condiciones ácidas de pH 5 o menor; (1) una etapa en la que se hace pasar un material que contiene trombomodulina soluble que contiene o se sospecha que contiene un producto desnaturalizado de la trombomodulina soluble, que se obtiene en la etapa (0) anterior, a través de un intercambiador aniónico o hidroxiapatita; y (2) un etapa en la que se obtiene una fracción que contiene trombomodulina soluble que no contiene sustancialmente un producto desnaturalizado de la trombomodulina soluble, en donde el contenido del producto desnaturalizado de la trombomodulina soluble en la trombomodulina soluble que no contiene sustancialmente el producto de trombomodulina soluble es 3% o menos, en condiciones de separación en la cuales la trombomodulina soluble puede ser separada de un producto desnaturalizado de la trombomodulina soluble.

Description

imagen1
imagen2
imagen3
imagen4
imagen5
imagen6
imagen7
imagen8
imagen9
imagen10
imagen11
imagen12
imagen13
imagen14
imagen15
imagen16
imagen17
imagen18
imagen19
imagen20

Claims (1)

  1. imagen1
    imagen2
ES08722575.1T 2007-03-23 2008-03-21 Método para producir trombomodulina soluble de gran pureza Active ES2496596T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2007077389 2007-03-23
JP2007077389 2007-03-23
PCT/JP2008/055211 WO2008117735A1 (ja) 2007-03-23 2008-03-21 高純度可溶性トロンボモジュリンの製造方法

Publications (1)

Publication Number Publication Date
ES2496596T3 true ES2496596T3 (es) 2014-09-19

Family

ID=39788470

Family Applications (1)

Application Number Title Priority Date Filing Date
ES08722575.1T Active ES2496596T3 (es) 2007-03-23 2008-03-21 Método para producir trombomodulina soluble de gran pureza

Country Status (12)

Country Link
US (2) US8258269B2 (es)
EP (1) EP2138505B1 (es)
JP (1) JP5117486B2 (es)
KR (1) KR101238047B1 (es)
CN (1) CN101641368B (es)
AU (1) AU2008230465B2 (es)
CA (1) CA2681294C (es)
ES (1) ES2496596T3 (es)
NZ (1) NZ579873A (es)
SG (1) SG184699A1 (es)
TW (2) TWI461434B (es)
WO (1) WO2008117735A1 (es)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011088225A1 (en) 2010-01-15 2011-07-21 Bio-Rad Laboratories, Inc. Surface neutralization of apatite
SG10201500036WA (en) * 2010-04-30 2015-02-27 Asahi Kasei Pharma Corp Highly-purified soluble thrombomodulin and method for producing same
US8895707B2 (en) 2010-08-18 2014-11-25 Bio-Rad Laboratories, Inc. Elution of proteins from hydroxyapatite resins without resin deterioration
JP5881744B2 (ja) 2011-02-02 2016-03-09 バイオ−ラッド ラボラトリーズ インコーポレーティッド アルカリ溶液でのアパタイトの表面中和方法
EP3456837A3 (en) * 2011-03-14 2019-04-24 Catalent Pharma Solutions, LLC Decorin compositions and use thereof
BR112014011488B1 (pt) 2011-11-15 2022-07-05 Asahi Kasei Pharma Corporation Medicamento para tratamento terapêutico e/ou melhora de sépsis
US9815695B2 (en) 2012-05-30 2017-11-14 Bio-Rad Laboratories, Inc. In situ restoration of apatite-based chromatography resins
US10099157B2 (en) 2014-06-23 2018-10-16 Bio-Rad Laboratories, Inc. Apatite in-situ restoration
CN105392569B (zh) 2014-06-23 2019-08-20 生物辐射实验室股份有限公司 磷灰石预处理
BR112021007460A2 (pt) 2018-10-22 2021-11-03 Asahi Kasei Pharma Corp Medicamento para tratamento terapêutico e/ou melhora de um paciente com sepse

Family Cites Families (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS62125173A (ja) 1985-11-26 1987-06-06 鹿島建設株式会社 免震構造物のフエ−ルセ−フ装置
EP0816495A1 (en) 1987-01-08 1998-01-07 Asahi Kasei Kogyo Kabushiki Kaisha DNA coding for a peptide promoting the activation of protein C by thrombin and dprocess for producing the same
JP2738428B2 (ja) 1987-01-08 1998-04-08 旭化成工業株式会社 トロンビンによるプロテインｃの活性化を促進する作用を有するペプチド
DK299087D0 (da) 1987-06-12 1987-06-12 Novo Industri As Proteins and derivatives thereof
JPS646219U (es) 1987-06-30 1989-01-13
JPH0720997B2 (ja) 1988-08-29 1995-03-08 興和株式会社 新規なトロンビン結合性物質及びその製法
JPH0798840B2 (ja) 1988-12-27 1995-10-25 持田製薬株式会社 尿由来の抗血液疑固物質、その製法およびそれを含有する医薬組成物
US5202421A (en) 1988-12-27 1993-04-13 Mochida Pharmaceutical Co., Ltd. Anticoagulant substance obtained from urine and process for the preparation thereof
JPH02255699A (ja) 1989-03-28 1990-10-16 Asahi Chem Ind Co Ltd 新規血液抗凝固物質及びその製法
US5466668A (en) 1989-04-28 1995-11-14 Schering Aktiengesellschaft Superior thrombomodulin analogs for pharmaceutical use
US6063763A (en) 1989-04-28 2000-05-16 Schering Aktiengesellschaft Protease-resistant thrombomodulin analogs
CA2022713A1 (en) 1989-08-11 1991-02-12 Nils U. Bang Human thrombomodulin derivatives
JPH03259084A (ja) 1990-03-06 1991-11-19 Kowa Co トロンビン結合性物質の製造法
AU643306B2 (en) 1990-06-27 1993-11-11 Mochida Pharmaceutical Co., Ltd. Anticoagulant polypeptides
JPH05213998A (ja) 1990-08-03 1993-08-24 Asahi Chem Ind Co Ltd 新規なポリペプチド及びこれを有効成分とする 医薬組成物
KR930008093B1 (ko) 1990-08-03 1993-08-25 아사히가세이고오교 가부시끼가이샤 신규 폴리펩티드 및 이를 유효성분으로 하는 의약조성물
CA2087271C (en) 1990-08-15 2009-03-24 Charles B. Glaser Superior thrombomodulin analogs for pharmaceutical use
JP3220174B2 (ja) 1990-12-12 2001-10-22 第一製薬株式会社 組換ヒトトロンボモジュリン誘導体
JP3517236B2 (ja) 1992-02-05 2004-04-12 シェーリング アクチェンゲゼルシャフト プロテアーゼ−耐性トロンボモジュリン・アナログ
JPH05310787A (ja) 1992-05-01 1993-11-22 Asahi Chem Ind Co Ltd 新規なポリペプチド
JP3007785B2 (ja) 1993-03-16 2000-02-07 旭化成工業株式会社 トロンボモジュリン組成物およびその変性防止方法
JPH06321085A (ja) 1993-05-18 1994-11-22 Mazda Motor Corp 車両のスリップ制御装置
JPH07173189A (ja) 1993-12-17 1995-07-11 Masashi Kosakai リガンド存在下で二段階に分けて行うヒドロキシアパタイトクロマトグラフィーの用法
JP3822383B2 (ja) 1993-12-17 2006-09-20 持田製薬株式会社 可溶性トロンボモジュリン含有組成物
JP3537440B2 (ja) 1993-12-17 2004-06-14 持田製薬株式会社 可溶性トロンボモジュリンを長期保存安定化させる方法
JPH11171790A (ja) 1994-03-15 1999-06-29 Asahi Chem Ind Co Ltd トロンボモジュリン用変性防止剤
JP2824392B2 (ja) 1994-05-31 1998-11-11 旭化成工業株式会社 トロンビンによるプロテインｃの活性化を促進する作用を有するペプチドを含有する培養上澄液
JP3745805B2 (ja) 1995-10-24 2006-02-15 日本ケミカルリサーチ株式会社 トロンボモジュリンの精製方法
KR100373480B1 (ko) * 1997-10-15 2003-02-25 아사히 가세이 가부시키가이샤 트롬보모듈린 수용액 주사제의 저장 및 유통시의 품질유지 방법
JP4157644B2 (ja) * 1998-03-30 2008-10-01 旭化成ファーマ株式会社 高純度可溶性トロンボモジュリンの製造方法
EP1500661A1 (en) 1998-06-01 2005-01-26 Genetech, Inc. Separation of protein monomers from aggregates by use of ion-exchange chromatography
EP1475098B1 (en) 2002-01-18 2015-08-05 Asahi Kasei Pharma Corporation High-concentration preparation of soluble thrombomodulin

Also Published As

Publication number Publication date
TW201431872A (zh) 2014-08-16
TWI461434B (zh) 2014-11-21
NZ579873A (en) 2011-11-25
AU2008230465B2 (en) 2011-10-13
CN101641368B (zh) 2013-05-08
CN101641368A (zh) 2010-02-03
SG184699A1 (en) 2012-10-30
AU2008230465A1 (en) 2008-10-02
JP5117486B2 (ja) 2013-01-16
US20130237693A1 (en) 2013-09-12
KR101238047B1 (ko) 2013-02-27
EP2138505B1 (en) 2014-08-06
US8258269B2 (en) 2012-09-04
WO2008117735A1 (ja) 2008-10-02
EP2138505A4 (en) 2011-02-09
TW200906848A (en) 2009-02-16
KR20090112709A (ko) 2009-10-28
US20100145020A1 (en) 2010-06-10
JPWO2008117735A1 (ja) 2010-07-15
CA2681294C (en) 2014-05-06
US8952137B2 (en) 2015-02-10
EP2138505A1 (en) 2009-12-30
CA2681294A1 (en) 2008-10-02
HK1137185A1 (en) 2010-07-23

Similar Documents

Publication Publication Date Title
ES2496596T3 (es) Método para producir trombomodulina soluble de gran pureza
ES2551711T3 (es) Proceso de maceración
ES2520841T3 (es) Procedimiento para la producción de partículas
AR065833A1 (es) Procedimiento para la produccion biologica de 1,3-propanodiol a partir de glicerina con un alto rendimiento
ES2565180T3 (es) Proceso para la preparación de ácido polisiálico de gran pureza
ES2546652T3 (es) Procedimiento para fabricar un cuerpo de moldeo rápidamente humectable
CO6290676A2 (es) Recuperacion de alcoholes superiores a partir de soluciones acuosas diluidas
ES2542692T3 (es) Procedimiento para la producción de biogás y aminoácidos a partir de un hidrolizado
BRPI0920619A8 (pt) Produção de soluções de proteína solúveis a partir de soja (''s701'')
MX2009013580A (es) Extracto que contiene equol, metodo para la produccion del mismo, metodo para la extraccion de equol, y alimento que contiene equol.
ATE389678T1 (de) Verfahren zur herstellung von wässrigen dispersionen
EA200970778A1 (ru) Способ получения эпихлоргидрина
AR044480A1 (es) Proceso para la produccion de carbonato de calcio precipitado en placas ,producto producido mediante el mismo ,y papel que lo incorpora
BR112012032208A2 (pt) fermentação de biomassa
AR059379A1 (es) Proceso y sistema para la produccion de alcohol por destilacion de efecto multiple, alcohol hidratado y proceso para producir alcohol anhidro a partir de este ultimo
MX382322B (es) Producto fermentado que contiene un microorganismo productor de equol, por el cual se mantiene la capacidad productiva de equol, y procedimiento para la producción del mismo.
CO5631483A2 (es) Metodo holografico para el etiquetado de productos
ES2567159T3 (es) Método para la deshidratación de alcoholes grasos
ES2634034T3 (es) Composiciones nutricionales con copos de fruta que contienen ácido docosahexenoico
Liu et al. Evaluation of quercetin in alleviating the negative effects of high soybean meal diet on spotted sea bass Lateolabrax maculatus
CN104560253A (zh) 一种沼气膜分离提纯二氧化碳气源热泵冷热联产技术
ES2531599T3 (es) Utilización de triglicéridos docosahexaenoicos para el tratamiento de enfermedades tumorales
AR057440A1 (es) Deshidrogenacion de alcoholes mezclados
ES2615878T3 (es) Procedimiento y aparato para la producción de un líquido mediante pirolisis
TW200726850A (en) Zirconium strip material and process for making same