JPH0160785B2 - - Google Patents
Info
- Publication number
- JPH0160785B2 JPH0160785B2 JP57193419A JP19341982A JPH0160785B2 JP H0160785 B2 JPH0160785 B2 JP H0160785B2 JP 57193419 A JP57193419 A JP 57193419A JP 19341982 A JP19341982 A JP 19341982A JP H0160785 B2 JPH0160785 B2 JP H0160785B2
- Authority
- JP
- Japan
- Prior art keywords
- support
- autoradiography
- stimulable phosphor
- phosphor
- phosphor sheet
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims description 143
- 239000000126 substance Substances 0.000 claims description 63
- 238000000376 autoradiography Methods 0.000 claims description 48
- 238000000926 separation method Methods 0.000 claims description 19
- 239000011230 binding agent Substances 0.000 claims description 17
- 238000005259 measurement Methods 0.000 claims description 17
- 238000011161 development Methods 0.000 claims description 16
- 229910052693 Europium Inorganic materials 0.000 claims description 9
- 229910052784 alkaline earth metal Inorganic materials 0.000 claims description 4
- 150000001342 alkaline earth metals Chemical group 0.000 claims description 4
- 238000004809 thin layer chromatography Methods 0.000 claims description 3
- OGPBJKLSAFTDLK-UHFFFAOYSA-N europium atom Chemical compound [Eu] OGPBJKLSAFTDLK-UHFFFAOYSA-N 0.000 claims description 2
- 239000010410 layer Substances 0.000 description 39
- 230000005855 radiation Effects 0.000 description 31
- 238000000034 method Methods 0.000 description 28
- 108020004414 DNA Proteins 0.000 description 17
- 239000011248 coating agent Substances 0.000 description 17
- 238000000576 coating method Methods 0.000 description 17
- 238000004020 luminiscence type Methods 0.000 description 17
- 239000000463 material Substances 0.000 description 17
- 239000000203 mixture Substances 0.000 description 15
- 239000000047 product Substances 0.000 description 14
- 239000002585 base Substances 0.000 description 13
- 229910052794 bromium Inorganic materials 0.000 description 13
- 239000000460 chlorine Substances 0.000 description 13
- 239000002245 particle Substances 0.000 description 13
- 229910052801 chlorine Inorganic materials 0.000 description 12
- 230000002285 radioactive effect Effects 0.000 description 11
- 239000000499 gel Substances 0.000 description 10
- 239000000243 solution Substances 0.000 description 10
- 238000000211 autoradiogram Methods 0.000 description 9
- 229910052740 iodine Inorganic materials 0.000 description 9
- 238000012545 processing Methods 0.000 description 9
- -1 DNA Chemical class 0.000 description 8
- 229910052791 calcium Inorganic materials 0.000 description 8
- 239000011575 calcium Substances 0.000 description 8
- 238000000354 decomposition reaction Methods 0.000 description 8
- 230000005284 excitation Effects 0.000 description 8
- 229910052751 metal Inorganic materials 0.000 description 8
- 239000002184 metal Substances 0.000 description 8
- 229920000728 polyester Polymers 0.000 description 8
- 229910052712 strontium Inorganic materials 0.000 description 8
- ZWEHNKRNPOVVGH-UHFFFAOYSA-N 2-Butanone Chemical compound CCC(C)=O ZWEHNKRNPOVVGH-UHFFFAOYSA-N 0.000 description 6
- 229910052771 Terbium Inorganic materials 0.000 description 6
- GWEVSGVZZGPLCZ-UHFFFAOYSA-N Titan oxide Chemical compound O=[Ti]=O GWEVSGVZZGPLCZ-UHFFFAOYSA-N 0.000 description 6
- 229910052793 cadmium Inorganic materials 0.000 description 6
- 229910052749 magnesium Inorganic materials 0.000 description 6
- 239000011777 magnesium Substances 0.000 description 6
- 230000001681 protective effect Effects 0.000 description 6
- 230000035945 sensitivity Effects 0.000 description 6
- 229910052725 zinc Inorganic materials 0.000 description 6
- 239000011701 zinc Substances 0.000 description 6
- 239000000020 Nitrocellulose Substances 0.000 description 5
- 229910052772 Samarium Inorganic materials 0.000 description 5
- 229920001220 nitrocellulos Polymers 0.000 description 5
- 230000003287 optical effect Effects 0.000 description 5
- 239000000941 radioactive substance Substances 0.000 description 5
- 229910052684 Cerium Inorganic materials 0.000 description 4
- 229910052691 Erbium Inorganic materials 0.000 description 4
- 229910052779 Neodymium Inorganic materials 0.000 description 4
- 229910052769 Ytterbium Inorganic materials 0.000 description 4
- 229910052790 beryllium Inorganic materials 0.000 description 4
- 238000001962 electrophoresis Methods 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 229910052736 halogen Inorganic materials 0.000 description 4
- 150000002367 halogens Chemical class 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- 238000002156 mixing Methods 0.000 description 4
- 108020004707 nucleic acids Proteins 0.000 description 4
- 102000039446 nucleic acids Human genes 0.000 description 4
- 150000007523 nucleic acids Chemical class 0.000 description 4
- 239000002985 plastic film Substances 0.000 description 4
- 229920000139 polyethylene terephthalate Polymers 0.000 description 4
- 239000005020 polyethylene terephthalate Substances 0.000 description 4
- 108090000623 proteins and genes Proteins 0.000 description 4
- 102000004169 proteins and genes Human genes 0.000 description 4
- 241001473992 Abax Species 0.000 description 3
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 3
- 229910052689 Holmium Inorganic materials 0.000 description 3
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 3
- 229910052775 Thulium Inorganic materials 0.000 description 3
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 3
- 229910052782 aluminium Inorganic materials 0.000 description 3
- QVQLCTNNEUAWMS-UHFFFAOYSA-N barium oxide Chemical compound [Ba]=O QVQLCTNNEUAWMS-UHFFFAOYSA-N 0.000 description 3
- ATBAMAFKBVZNFJ-UHFFFAOYSA-N beryllium atom Chemical compound [Be] ATBAMAFKBVZNFJ-UHFFFAOYSA-N 0.000 description 3
- BDOSMKKIYDKNTQ-UHFFFAOYSA-N cadmium atom Chemical compound [Cd] BDOSMKKIYDKNTQ-UHFFFAOYSA-N 0.000 description 3
- 239000006229 carbon black Substances 0.000 description 3
- 238000009125 cardiac resynchronization therapy Methods 0.000 description 3
- 239000000470 constituent Substances 0.000 description 3
- 229920001577 copolymer Polymers 0.000 description 3
- 239000007857 degradation product Substances 0.000 description 3
- MTHSVFCYNBDYFN-UHFFFAOYSA-N diethylene glycol Chemical compound OCCOCCO MTHSVFCYNBDYFN-UHFFFAOYSA-N 0.000 description 3
- 239000011888 foil Substances 0.000 description 3
- 238000001502 gel electrophoresis Methods 0.000 description 3
- 239000011521 glass Substances 0.000 description 3
- 229910052746 lanthanum Inorganic materials 0.000 description 3
- CIOAGBVUUVVLOB-UHFFFAOYSA-N strontium atom Chemical compound [Sr] CIOAGBVUUVVLOB-UHFFFAOYSA-N 0.000 description 3
- 229910052716 thallium Inorganic materials 0.000 description 3
- 239000004408 titanium dioxide Substances 0.000 description 3
- WKBOTKDWSSQWDR-UHFFFAOYSA-N Bromine atom Chemical compound [Br] WKBOTKDWSSQWDR-UHFFFAOYSA-N 0.000 description 2
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 2
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
- 229910052692 Dysprosium Inorganic materials 0.000 description 2
- 229910052688 Gadolinium Inorganic materials 0.000 description 2
- 108010010803 Gelatin Proteins 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 2
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 description 2
- 239000004952 Polyamide Substances 0.000 description 2
- 239000004372 Polyvinyl alcohol Substances 0.000 description 2
- YSMRWXYRXBRSND-UHFFFAOYSA-N TOTP Chemical compound CC1=CC=CC=C1OP(=O)(OC=1C(=CC=CC=1)C)OC1=CC=CC=C1C YSMRWXYRXBRSND-UHFFFAOYSA-N 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 239000000853 adhesive Substances 0.000 description 2
- 230000001070 adhesive effect Effects 0.000 description 2
- WNLRTRBMVRJNCN-UHFFFAOYSA-N adipic acid Chemical compound OC(=O)CCCCC(O)=O WNLRTRBMVRJNCN-UHFFFAOYSA-N 0.000 description 2
- 229910052783 alkali metal Inorganic materials 0.000 description 2
- 150000001340 alkali metals Chemical class 0.000 description 2
- 230000003321 amplification Effects 0.000 description 2
- 229910052788 barium Inorganic materials 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- GDTBXPJZTBHREO-UHFFFAOYSA-N bromine Substances BrBr GDTBXPJZTBHREO-UHFFFAOYSA-N 0.000 description 2
- 239000000872 buffer Substances 0.000 description 2
- 229910052792 caesium Inorganic materials 0.000 description 2
- 229920002301 cellulose acetate Polymers 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical compound NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 description 2
- FLKPEMZONWLCSK-UHFFFAOYSA-N diethyl phthalate Chemical compound CCOC(=O)C1=CC=CC=C1C(=O)OCC FLKPEMZONWLCSK-UHFFFAOYSA-N 0.000 description 2
- 239000002270 dispersing agent Substances 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000012634 fragment Substances 0.000 description 2
- 230000006870 function Effects 0.000 description 2
- 229910052733 gallium Inorganic materials 0.000 description 2
- 229920000159 gelatin Polymers 0.000 description 2
- 239000008273 gelatin Substances 0.000 description 2
- 235000019322 gelatine Nutrition 0.000 description 2
- 235000011852 gelatine desserts Nutrition 0.000 description 2
- UYTPUPDQBNUYGX-UHFFFAOYSA-N guanine Chemical compound O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 description 2
- FUZZWVXGSFPDMH-UHFFFAOYSA-N hexanoic acid Chemical compound CCCCCC(O)=O FUZZWVXGSFPDMH-UHFFFAOYSA-N 0.000 description 2
- 229910052739 hydrogen Inorganic materials 0.000 description 2
- 239000001257 hydrogen Substances 0.000 description 2
- 229910052738 indium Inorganic materials 0.000 description 2
- 229910052745 lead Inorganic materials 0.000 description 2
- 229910052744 lithium Inorganic materials 0.000 description 2
- 238000003199 nucleic acid amplification method Methods 0.000 description 2
- XNGIFLGASWRNHJ-UHFFFAOYSA-N phthalic acid Chemical compound OC(=O)C1=CC=CC=C1C(O)=O XNGIFLGASWRNHJ-UHFFFAOYSA-N 0.000 description 2
- 239000000049 pigment Substances 0.000 description 2
- 229920006255 plastic film Polymers 0.000 description 2
- 239000004014 plasticizer Substances 0.000 description 2
- 229920003229 poly(methyl methacrylate) Polymers 0.000 description 2
- 229920002037 poly(vinyl butyral) polymer Polymers 0.000 description 2
- 229920002647 polyamide Polymers 0.000 description 2
- 229920000515 polycarbonate Polymers 0.000 description 2
- 239000004417 polycarbonate Substances 0.000 description 2
- 239000004926 polymethyl methacrylate Substances 0.000 description 2
- 229920002689 polyvinyl acetate Polymers 0.000 description 2
- 239000011118 polyvinyl acetate Substances 0.000 description 2
- 229920002451 polyvinyl alcohol Polymers 0.000 description 2
- 229910052700 potassium Inorganic materials 0.000 description 2
- 229920005989 resin Polymers 0.000 description 2
- 239000011347 resin Substances 0.000 description 2
- 108091008146 restriction endonucleases Proteins 0.000 description 2
- 229910052701 rubidium Inorganic materials 0.000 description 2
- 229910052706 scandium Inorganic materials 0.000 description 2
- 238000012163 sequencing technique Methods 0.000 description 2
- 229910052708 sodium Inorganic materials 0.000 description 2
- 239000002904 solvent Substances 0.000 description 2
- KDYFGRWQOYBRFD-UHFFFAOYSA-N succinic acid Chemical compound OC(=O)CCC(O)=O KDYFGRWQOYBRFD-UHFFFAOYSA-N 0.000 description 2
- RWQNBRDOKXIBIV-UHFFFAOYSA-N thymine Chemical compound CC1=CNC(=O)NC1=O RWQNBRDOKXIBIV-UHFFFAOYSA-N 0.000 description 2
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 2
- 229910052727 yttrium Inorganic materials 0.000 description 2
- PZBLUWVMZMXIKZ-UHFFFAOYSA-N 2-o-(2-ethoxy-2-oxoethyl) 1-o-ethyl benzene-1,2-dicarboxylate Chemical compound CCOC(=O)COC(=O)C1=CC=CC=C1C(=O)OCC PZBLUWVMZMXIKZ-UHFFFAOYSA-N 0.000 description 1
- QGHDLJAZIIFENW-UHFFFAOYSA-N 4-[1,1,1,3,3,3-hexafluoro-2-(4-hydroxy-3-prop-2-enylphenyl)propan-2-yl]-2-prop-2-enylphenol Chemical group C1=C(CC=C)C(O)=CC=C1C(C(F)(F)F)(C(F)(F)F)C1=CC=C(O)C(CC=C)=C1 QGHDLJAZIIFENW-UHFFFAOYSA-N 0.000 description 1
- ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical compound [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 description 1
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 description 1
- 229930024421 Adenine Natural products 0.000 description 1
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 1
- 229910018072 Al 2 O 3 Inorganic materials 0.000 description 1
- 229910000838 Al alloy Inorganic materials 0.000 description 1
- GOJCZVPJCKEBQV-UHFFFAOYSA-N Butyl phthalyl butylglycolate Chemical compound CCCCOC(=O)COC(=O)C1=CC=CC=C1C(=O)OCCCC GOJCZVPJCKEBQV-UHFFFAOYSA-N 0.000 description 1
- 239000004215 Carbon black (E152) Substances 0.000 description 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 1
- 102000053602 DNA Human genes 0.000 description 1
- 229920002307 Dextran Polymers 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 239000001856 Ethyl cellulose Substances 0.000 description 1
- ZZSNKZQZMQGXPY-UHFFFAOYSA-N Ethyl cellulose Chemical compound CCOCC1OC(OC)C(OCC)C(OCC)C1OC1C(O)C(O)C(OC)C(CO)O1 ZZSNKZQZMQGXPY-UHFFFAOYSA-N 0.000 description 1
- 229910005793 GeO 2 Inorganic materials 0.000 description 1
- AEMRFAOFKBGASW-UHFFFAOYSA-N Glycolic acid Chemical class OCC(O)=O AEMRFAOFKBGASW-UHFFFAOYSA-N 0.000 description 1
- 229920000084 Gum arabic Polymers 0.000 description 1
- 229910021193 La 2 O 3 Inorganic materials 0.000 description 1
- 229910052765 Lutetium Inorganic materials 0.000 description 1
- 206010034960 Photophobia Diseases 0.000 description 1
- 239000004698 Polyethylene Substances 0.000 description 1
- 239000002202 Polyethylene glycol Substances 0.000 description 1
- 239000004642 Polyimide Substances 0.000 description 1
- 229920001328 Polyvinylidene chloride Polymers 0.000 description 1
- 229910052777 Praseodymium Inorganic materials 0.000 description 1
- 241000978776 Senegalia senegal Species 0.000 description 1
- 229910004298 SiO 2 Inorganic materials 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 229910006404 SnO 2 Inorganic materials 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 235000021355 Stearic acid Nutrition 0.000 description 1
- 229910010413 TiO 2 Inorganic materials 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- BZHJMEDXRYGGRV-UHFFFAOYSA-N Vinyl chloride Chemical compound ClC=C BZHJMEDXRYGGRV-UHFFFAOYSA-N 0.000 description 1
- QCWXUUIWCKQGHC-UHFFFAOYSA-N Zirconium Chemical compound [Zr] QCWXUUIWCKQGHC-UHFFFAOYSA-N 0.000 description 1
- GEIAQOFPUVMAGM-UHFFFAOYSA-N ZrO Inorganic materials [Zr]=O GEIAQOFPUVMAGM-UHFFFAOYSA-N 0.000 description 1
- FJWGYAHXMCUOOM-QHOUIDNNSA-N [(2s,3r,4s,5r,6r)-2-[(2r,3r,4s,5r,6s)-4,5-dinitrooxy-2-(nitrooxymethyl)-6-[(2r,3r,4s,5r,6s)-4,5,6-trinitrooxy-2-(nitrooxymethyl)oxan-3-yl]oxyoxan-3-yl]oxy-3,5-dinitrooxy-6-(nitrooxymethyl)oxan-4-yl] nitrate Chemical compound O([C@@H]1O[C@@H]([C@H]([C@H](O[N+]([O-])=O)[C@H]1O[N+]([O-])=O)O[C@H]1[C@@H]([C@@H](O[N+]([O-])=O)[C@H](O[N+]([O-])=O)[C@@H](CO[N+]([O-])=O)O1)O[N+]([O-])=O)CO[N+](=O)[O-])[C@@H]1[C@@H](CO[N+]([O-])=O)O[C@@H](O[N+]([O-])=O)[C@H](O[N+]([O-])=O)[C@H]1O[N+]([O-])=O FJWGYAHXMCUOOM-QHOUIDNNSA-N 0.000 description 1
- 230000009102 absorption Effects 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000000205 acacia gum Substances 0.000 description 1
- 235000010489 acacia gum Nutrition 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- NIXOWILDQLNWCW-UHFFFAOYSA-N acrylic acid group Chemical group C(C=C)(=O)O NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 229960000643 adenine Drugs 0.000 description 1
- 239000012790 adhesive layer Substances 0.000 description 1
- 239000001361 adipic acid Substances 0.000 description 1
- 235000011037 adipic acid Nutrition 0.000 description 1
- 125000001931 aliphatic group Chemical group 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 229910052785 arsenic Inorganic materials 0.000 description 1
- RQNWIZPPADIBDY-UHFFFAOYSA-N arsenic atom Chemical compound [As] RQNWIZPPADIBDY-UHFFFAOYSA-N 0.000 description 1
- 229910001864 baryta Inorganic materials 0.000 description 1
- 229920001222 biopolymer Polymers 0.000 description 1
- HSUIVCLOAAJSRE-UHFFFAOYSA-N bis(2-methoxyethyl) benzene-1,2-dicarboxylate Chemical compound COCCOC(=O)C1=CC=CC=C1C(=O)OCCOC HSUIVCLOAAJSRE-UHFFFAOYSA-N 0.000 description 1
- 229910052797 bismuth Inorganic materials 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920006217 cellulose acetate butyrate Polymers 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 125000001309 chloro group Chemical group Cl* 0.000 description 1
- 229910052804 chromium Inorganic materials 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 229940104302 cytosine Drugs 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- ASMQGLCHMVWBQR-UHFFFAOYSA-M diphenyl phosphate Chemical compound C=1C=CC=CC=1OP(=O)([O-])OC1=CC=CC=C1 ASMQGLCHMVWBQR-UHFFFAOYSA-M 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- VHJLVAABSRFDPM-QWWZWVQMSA-N dithiothreitol Chemical compound SC[C@@H](O)[C@H](O)CS VHJLVAABSRFDPM-QWWZWVQMSA-N 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- 125000002573 ethenylidene group Chemical group [*]=C=C([H])[H] 0.000 description 1
- 229920001249 ethyl cellulose Polymers 0.000 description 1
- 235000019325 ethyl cellulose Nutrition 0.000 description 1
- 230000029142 excretion Effects 0.000 description 1
- 229910052731 fluorine Inorganic materials 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 229930195733 hydrocarbon Natural products 0.000 description 1
- 150000002430 hydrocarbons Chemical class 0.000 description 1
- PNDPGZBMCMUPRI-UHFFFAOYSA-N iodine Chemical compound II PNDPGZBMCMUPRI-UHFFFAOYSA-N 0.000 description 1
- 239000011630 iodine Substances 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 150000002576 ketones Chemical class 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 208000013469 light sensitivity Diseases 0.000 description 1
- 229910001629 magnesium chloride Inorganic materials 0.000 description 1
- 229910052748 manganese Inorganic materials 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 229910044991 metal oxide Inorganic materials 0.000 description 1
- 150000004706 metal oxides Chemical class 0.000 description 1
- 150000002739 metals Chemical class 0.000 description 1
- 238000013508 migration Methods 0.000 description 1
- 230000005012 migration Effects 0.000 description 1
- 238000000465 moulding Methods 0.000 description 1
- 229910052759 nickel Inorganic materials 0.000 description 1
- QIQXTHQIDYTFRH-UHFFFAOYSA-N octadecanoic acid Chemical compound CCCCCCCCCCCCCCCCCC(O)=O QIQXTHQIDYTFRH-UHFFFAOYSA-N 0.000 description 1
- OQCDKBAXFALNLD-UHFFFAOYSA-N octadecanoic acid Natural products CCCCCCCC(C)CCCCCCCCC(O)=O OQCDKBAXFALNLD-UHFFFAOYSA-N 0.000 description 1
- 150000003014 phosphoric acid esters Chemical class 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 150000003021 phthalic acid derivatives Chemical class 0.000 description 1
- 239000013612 plasmid Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 229920001225 polyester resin Polymers 0.000 description 1
- 239000004645 polyester resin Substances 0.000 description 1
- 229920000573 polyethylene Polymers 0.000 description 1
- 229920001223 polyethylene glycol Polymers 0.000 description 1
- 229920001721 polyimide Polymers 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 239000002861 polymer material Substances 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 229920002635 polyurethane Polymers 0.000 description 1
- 239000004814 polyurethane Substances 0.000 description 1
- 239000005033 polyvinylidene chloride Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000000163 radioactive labelling Methods 0.000 description 1
- 239000000700 radioactive tracer Substances 0.000 description 1
- 238000002601 radiography Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- SIXSYDAISGFNSX-UHFFFAOYSA-N scandium atom Chemical compound [Sc] SIXSYDAISGFNSX-UHFFFAOYSA-N 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
- 239000010703 silicon Substances 0.000 description 1
- GGCZERPQGJTIQP-UHFFFAOYSA-N sodium;9,10-dioxoanthracene-2-sulfonic acid Chemical compound [Na+].C1=CC=C2C(=O)C3=CC(S(=O)(=O)O)=CC=C3C(=O)C2=C1 GGCZERPQGJTIQP-UHFFFAOYSA-N 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008117 stearic acid Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000001384 succinic acid Substances 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 229920001059 synthetic polymer Polymers 0.000 description 1
- 229920003002 synthetic resin Polymers 0.000 description 1
- 239000000057 synthetic resin Substances 0.000 description 1
- 239000013076 target substance Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 229940113082 thymine Drugs 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 229910052723 transition metal Inorganic materials 0.000 description 1
- 150000003624 transition metals Chemical class 0.000 description 1
- 238000013519 translation Methods 0.000 description 1
- 229920006352 transparent thermoplastic Polymers 0.000 description 1
- 238000011282 treatment Methods 0.000 description 1
- ILJSQTXMGCGYMG-UHFFFAOYSA-N triacetic acid Chemical compound CC(=O)CC(=O)CC(O)=O ILJSQTXMGCGYMG-UHFFFAOYSA-N 0.000 description 1
- ZIBGPFATKBEMQZ-UHFFFAOYSA-N triethylene glycol Chemical compound OCCOCCOCCO ZIBGPFATKBEMQZ-UHFFFAOYSA-N 0.000 description 1
- XZZNDPSIHUTMOC-UHFFFAOYSA-N triphenyl phosphate Chemical compound C=1C=CC=CC=1OP(OC=1C=CC=CC=1)(=O)OC1=CC=CC=C1 XZZNDPSIHUTMOC-UHFFFAOYSA-N 0.000 description 1
- 229910052720 vanadium Inorganic materials 0.000 description 1
- 229920002554 vinyl polymer Polymers 0.000 description 1
- 229910052726 zirconium Inorganic materials 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/60—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances involving radioactive labelled substances
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01T—MEASUREMENT OF NUCLEAR OR X-RADIATION
- G01T1/00—Measuring X-radiation, gamma radiation, corpuscular radiation, or cosmic radiation
- G01T1/16—Measuring radiation intensity
- G01T1/20—Measuring radiation intensity with scintillation detectors
- G01T1/2012—Measuring radiation intensity with scintillation detectors using stimulable phosphors, e.g. stimulable phosphor sheets
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- General Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Immunology (AREA)
- Biomedical Technology (AREA)
- Physics & Mathematics (AREA)
- Urology & Nephrology (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Food Science & Technology (AREA)
- Pathology (AREA)
- High Energy & Nuclear Physics (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Measurement Of Radiation (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Conversion Of X-Rays Into Visible Images (AREA)
- Radiography Using Non-Light Waves (AREA)
Description
ãçºæã®è©³çŽ°ãªèª¬æã
æ¬çºæã¯ããªãŒãã©ãžãªã°ã©ãã€ãŒçšæž¬å®ãã
ãã«é¢ãããã®ã§ãããæŽã«è©³ããã¯ãæ¬çºæ
ã¯ãæŸå°æ§æšèãä»ãããŠããçç©äœç±æ¥ã®ç©è³ª
ã®æ··åç©ãããªãè©Šæããæ¯æäœãçšããŠåé¢å±
éãã次ãã§ããã®æ¯æäœäžã§åé¢å±éãããå
ç©è³ªã®äžæ¬¡å çãããã¯äºæ¬¡å çãªäœçœ®æ å ±ãã
該ç©è³ªã®æŸå°æ§ãå©çšããŠæ€åºæž¬å®ããããšã«ã
ãããããã®ç©è³ªã®åé¢å±éãåå®ãªã©ãè¡ãªã
ããã«æå¹ã«å©çšããããªãŒãã©ãžãªã°ã©ãã€ãŒ
çšã®æž¬å®ãããã«é¢ãããã®ã§ããã
ãã«é¢ãããã®ã§ãããæŽã«è©³ããã¯ãæ¬çºæ
ã¯ãæŸå°æ§æšèãä»ãããŠããçç©äœç±æ¥ã®ç©è³ª
ã®æ··åç©ãããªãè©Šæããæ¯æäœãçšããŠåé¢å±
éãã次ãã§ããã®æ¯æäœäžã§åé¢å±éãããå
ç©è³ªã®äžæ¬¡å çãããã¯äºæ¬¡å çãªäœçœ®æ å ±ãã
該ç©è³ªã®æŸå°æ§ãå©çšããŠæ€åºæž¬å®ããããšã«ã
ãããããã®ç©è³ªã®åé¢å±éãåå®ãªã©ãè¡ãªã
ããã«æå¹ã«å©çšããããªãŒãã©ãžãªã°ã©ãã€ãŒ
çšã®æž¬å®ãããã«é¢ãããã®ã§ããã
æŸå°æ§æšèãä»äžããç©è³ªãçç©äœã«æäžãã
ã®ã¡ããã®çç©äœããããã¯ããã®çç©äœã®çµç¹
ã®äžéšãè©Šæãšãããã®è©Šæãšé«æ床ç·ãã€ã«
ã ãªã©ã®æŸå°ç·ãã€ã«ã ãšãäžå®æééãåãã
ããšã«ãã€ãŠã該ãã€ã«ã ãæå ïŒãããã¯ãé²
å ïŒããããã®æå éšäœãã該詊æäžã«ãããæŸ
å°æ§æšèç©è³ªã®äœçœ®æ å ±ãåŸãããšãããªããªãŒ
ãã©ãžãªã°ã©ãã€ãŒïŒã©ãžãªãªãŒãã°ã©ãã€ãŒãš
ãåŒã°ããïŒã¯ãåŸæ¥ããç¥ãããŠããããã®ãª
ãŒãã©ãžãªã°ã©ãã€ãŒã¯ãããšãã°ãçç©äœã«ã
ããæäžç©è³ªã®ä»£è¬ãåžåãææ³ã®çµè·¯ãç¶æ ãª
ã©ã詳ããç 究ããããã«å©çšãããŠããããã®
ãããªãªãŒãã©ãžãªã°ã©ãã€ãŒã«ã€ããŠã¯ãããš
ãã°ã次ã«ç€ºãæç®ã«èšèŒãããã
ã®ã¡ããã®çç©äœããããã¯ããã®çç©äœã®çµç¹
ã®äžéšãè©Šæãšãããã®è©Šæãšé«æ床ç·ãã€ã«
ã ãªã©ã®æŸå°ç·ãã€ã«ã ãšãäžå®æééãåãã
ããšã«ãã€ãŠã該ãã€ã«ã ãæå ïŒãããã¯ãé²
å ïŒããããã®æå éšäœãã該詊æäžã«ãããæŸ
å°æ§æšèç©è³ªã®äœçœ®æ å ±ãåŸãããšãããªããªãŒ
ãã©ãžãªã°ã©ãã€ãŒïŒã©ãžãªãªãŒãã°ã©ãã€ãŒãš
ãåŒã°ããïŒã¯ãåŸæ¥ããç¥ãããŠããããã®ãª
ãŒãã©ãžãªã°ã©ãã€ãŒã¯ãããšãã°ãçç©äœã«ã
ããæäžç©è³ªã®ä»£è¬ãåžåãææ³ã®çµè·¯ãç¶æ ãª
ã©ã詳ããç 究ããããã«å©çšãããŠããããã®
ãããªãªãŒãã©ãžãªã°ã©ãã€ãŒã«ã€ããŠã¯ãããš
ãã°ã次ã«ç€ºãæç®ã«èšèŒãããã
çååŠå®éšè¬åº§ïŒ ãã¬ãŒãµãŒå®éšæ³ïŒäžïŒ
271ã289é ãã8.ãªãŒãã©ãžãªã°ã©ãã€ãŒãæ«å
培ãéæŸæäžïŒ1977幎ã(æ ª)æ±äº¬ååŠå人åïŒ ãŸãè¿å¹Žã§ã¯ããªãŒãã©ãžãªã°ã©ãã€ãŒã¯ãæŸ
å°æ§æšèãä»äžãããçç©äœã®çµç¹ããã³ïŒãŸã
ã¯çç©äœç±æ¥ã®ç©è³ªãå«ãæ¯æäœã«ãããæŸå°æ§
æšèç©è³ªã®äœçœ®æ å ±ãåŸãããã«ãæå¹ã«å©çšã
ããŠããã
271ã289é ãã8.ãªãŒãã©ãžãªã°ã©ãã€ãŒãæ«å
培ãéæŸæäžïŒ1977幎ã(æ ª)æ±äº¬ååŠå人åïŒ ãŸãè¿å¹Žã§ã¯ããªãŒãã©ãžãªã°ã©ãã€ãŒã¯ãæŸ
å°æ§æšèãä»äžãããçç©äœã®çµç¹ããã³ïŒãŸã
ã¯çç©äœç±æ¥ã®ç©è³ªãå«ãæ¯æäœã«ãããæŸå°æ§
æšèç©è³ªã®äœçœ®æ å ±ãåŸãããã«ãæå¹ã«å©çšã
ããŠããã
ããšãã°ãèçœè³ªãæ žé
žãªã©ã®ãããªçç©äœç±
æ¥ã®é«ååç©è³ªã«æŸå°æ§æšèãä»äžãããã®æŸå°
æ§æšèé«ååç©è³ªããã®èªå°äœããããã¯ãã®å
解ç©ãªã©ãã²ã«é»æ°æ³³åãªã©ã®åé¢å±éæäœã«ã
ããŠã²ã«ç¶æ¯æäœã«ãããŠåé¢å±éãããã®ã²ã«
ç¶æ¯æäœãšé«æ床ç·ãã€ã«ã ãšãäžå®æééã
åãããããšã«ããã該ãã€ã«ã ãæå ãããã
ã®æå éšäœããåŸããã該ã²ã«äžã«ãããæŸå°æ§
æšèç©è³ªã®äœçœ®æ å ±ãåºã«ããŠããã®é«ååç©è³ª
ã®åé¢ãåå®ããããã¯é«ååç©è³ªã®ååéãç¹
æ§ã®è©äŸ¡ãªã©ãè¡ãªãæ¹æ³ãéçºãããå®éã«å©
çšãããŠããã
æ¥ã®é«ååç©è³ªã«æŸå°æ§æšèãä»äžãããã®æŸå°
æ§æšèé«ååç©è³ªããã®èªå°äœããããã¯ãã®å
解ç©ãªã©ãã²ã«é»æ°æ³³åãªã©ã®åé¢å±éæäœã«ã
ããŠã²ã«ç¶æ¯æäœã«ãããŠåé¢å±éãããã®ã²ã«
ç¶æ¯æäœãšé«æ床ç·ãã€ã«ã ãšãäžå®æééã
åãããããšã«ããã該ãã€ã«ã ãæå ãããã
ã®æå éšäœããåŸããã該ã²ã«äžã«ãããæŸå°æ§
æšèç©è³ªã®äœçœ®æ å ±ãåºã«ããŠããã®é«ååç©è³ª
ã®åé¢ãåå®ããããã¯é«ååç©è³ªã®ååéãç¹
æ§ã®è©äŸ¡ãªã©ãè¡ãªãæ¹æ³ãéçºãããå®éã«å©
çšãããŠããã
ãã®ãããªãªãŒãã©ãžãªã°ã©ãã€ãŒã«ã€ããŠã¯
ããšãã°ã次ã«ç€ºãæç®ã«èšèŒãããŠããã
ããšãã°ã次ã«ç€ºãæç®ã«èšèŒãããŠããã
ãElECTROPHORESIS OF PROTEINS IN
POLYACRY LAMIDE AND STARCH
GELSãïŒA.H.GordonïŒNorthâHolland
Publishing CompanyïŒAmsterdam 1969ïŒïŒéŠ
èš³ãã²ã«é»æ°æ³³åæ³ãæ±äº¬ååŠå人çºè¡ 1974ã ç¹ã«è¿å¹Žã«ãããŠã¯ããªãŒãã©ãžãªã°ã©ãã€ãŒ
DNAãªã©ã®æ žé žã®å¡©åºé åã®æ±ºå®ã«ãæå¹ã«å©
çšãããŠãããåŸã€ãŠçç©äœã«ç±æ¥ããé«ååç©
質ã®æ§é 決å®ã«ãããŠéåžžã«æçšãªæ段ãšãªã€ãŠ
ããã
POLYACRY LAMIDE AND STARCH
GELSãïŒA.H.GordonïŒNorthâHolland
Publishing CompanyïŒAmsterdam 1969ïŒïŒéŠ
èš³ãã²ã«é»æ°æ³³åæ³ãæ±äº¬ååŠå人çºè¡ 1974ã ç¹ã«è¿å¹Žã«ãããŠã¯ããªãŒãã©ãžãªã°ã©ãã€ãŒ
DNAãªã©ã®æ žé žã®å¡©åºé åã®æ±ºå®ã«ãæå¹ã«å©
çšãããŠãããåŸã€ãŠçç©äœã«ç±æ¥ããé«ååç©
質ã®æ§é 決å®ã«ãããŠéåžžã«æçšãªæ段ãšãªã€ãŠ
ããã
ãã®ãªãŒãã©ãžãªã°ã©ãã€ãŒãå©çšããããšã«
ããDNAã®å¡©åºé åã決å®ããæ¹æ³ãšããŠã¯ã
ãããµã ã»ã®ã«ããŒãïŒMaxamâGilbertïŒæ³ã
ããã³ãµã³ã¬ãŒã»ã¯ãŒã«ãœã³ïŒSangerâ
CoulsonïŒæ³ãç¥ãããŠããããããã®æ¹æ³ã¯ã
DNAãäºéã©ã»ã³æ§é ãæãããã€ããã®äºé
ã©ã»ã³ã圢æããäºæ¬ã®éç¶ååéã®çµåããã
ã®ååã®æ§æåäœã§ããå€æ°ã®å¡©åºéã®æ°ŽçŽ çµå
ã«èµ·å ããããšããããŠããã®å€æ°ã®æ§æå¡©åºå
äœã¯ãã¢ããã³(A)ãã°ã¢ãã³ïŒïŒ§ïŒãã·ãã·ã³(C)
ãããŠããã³ïŒïŒŽïŒã®åçš®é¡ã®å¡©åºã®ã¿ãããª
ãããã€åæ§æå¡©åºåäœã®éã®æ°ŽçŽ çµåã¯ãâ
ããã³ïŒ¡âã®äºçš®é¡ã®çµåããã®ã¿ã«ãããŠ
å®çŸããŠãããšããDNAã®ç¹åŸŽçãªæ§é ãå·§åŠ
ã«å©çšããŠããã®å¡©åºé åã決å®ããæ¹æ³ã§ã
ãã
ããDNAã®å¡©åºé åã決å®ããæ¹æ³ãšããŠã¯ã
ãããµã ã»ã®ã«ããŒãïŒMaxamâGilbertïŒæ³ã
ããã³ãµã³ã¬ãŒã»ã¯ãŒã«ãœã³ïŒSangerâ
CoulsonïŒæ³ãç¥ãããŠããããããã®æ¹æ³ã¯ã
DNAãäºéã©ã»ã³æ§é ãæãããã€ããã®äºé
ã©ã»ã³ã圢æããäºæ¬ã®éç¶ååéã®çµåããã
ã®ååã®æ§æåäœã§ããå€æ°ã®å¡©åºéã®æ°ŽçŽ çµå
ã«èµ·å ããããšããããŠããã®å€æ°ã®æ§æå¡©åºå
äœã¯ãã¢ããã³(A)ãã°ã¢ãã³ïŒïŒ§ïŒãã·ãã·ã³(C)
ãããŠããã³ïŒïŒŽïŒã®åçš®é¡ã®å¡©åºã®ã¿ãããª
ãããã€åæ§æå¡©åºåäœã®éã®æ°ŽçŽ çµåã¯ãâ
ããã³ïŒ¡âã®äºçš®é¡ã®çµåããã®ã¿ã«ãããŠ
å®çŸããŠãããšããDNAã®ç¹åŸŽçãªæ§é ãå·§åŠ
ã«å©çšããŠããã®å¡©åºé åã決å®ããæ¹æ³ã§ã
ãã
ããšãã°ããããµã ã»ã®ã«ããŒãæ³ã¯æ¬¡ã«èšèŒ
ãããããªæäœã«ããå®æœããã
ãããããªæäœã«ããå®æœããã
å¡©åºé
åã決å®ããããšããŠããDNAããã
ã¯DNAã®å解ç©ã®éç¶ååã®äžæ¹ã®åŽã®ç«¯éšã«
çïŒïŒ°ïŒã®æŸå°æ§åäœå çŽ ãå«ãåºãçµåããã
ããšã«ããããã®å¯Ÿè±¡ç©ãæŸå°æ§æšèç©è³ªãšãã
ã®ã¡ãååŠçæ段ãå©çšããŠéç¶ååã®åå¡©åºã®
éã®çµåãç¹ç°çã«åæããã次ã«ããã®æäœã«
ããåŸãããDNAãããã¯DNAã®å解ç©ã®å€æ°
ã®åæå解ç©ã®æ··åç©ãã²ã«é»æ°æ³³åæ³ã«ããå
é¢å±éããå€æ°ã®åæå解ç©ããããã垯ç¶ã圢
æããŠåé¢å±éãããã©ãžãªã¯ãããã°ã©ã ïŒã
ã ããèŠèŠçã«ã¯èŠãããšãã§ããªãïŒãåŸãã
ãšãã§ããããã®ã©ãžãªã¯ãããã°ã©ã ãšé«æ床
ç·ãã€ã«ã ãšãäœæž©äžã«ãŠé·æééãåãããŠ
ãããšãæŸå°æ§åäœå çŽ ãååäžã«å«ãåæå解
ç©ãååšããäœçœ®ã«é¢ããç·ãã€ã«ã ã®éšåã¯
æå ããŠæœåã圢æããããã®ããã«ããŠæœåã
圢æããç·ãã€ã«ã ãçŸåããããšã«ããã©ãž
ãªã¯ãããã°ã©ã ã«å¯Ÿå¿ããå€æ°ã®åž¯ç¶åž¯åãå«
ãã¯ãããã°ã©ã ãç·ãã€ã«ã äžã«å¯èŠåãšã
ãŠçŸãããããããŠããã®å¯èŠåãããã¯ããã
ã°ã©ã ãšç¹ç°çåææ段ãšãããæŸå°æ§åäœå çŽ
ãçµåãããéç¶ååã®ç«¯éšããäžå®ã®äœçœ®é¢ä¿
ã«ããå¡©åºãé 次決å®ããããšãã§ãããã®ãã
ã«ããŠå¯Ÿè±¡ç©ã®ãã¹ãŠã®å¡©åºã®é åã決å®ããã
ãšãã§ããã
ã¯DNAã®å解ç©ã®éç¶ååã®äžæ¹ã®åŽã®ç«¯éšã«
çïŒïŒ°ïŒã®æŸå°æ§åäœå çŽ ãå«ãåºãçµåããã
ããšã«ããããã®å¯Ÿè±¡ç©ãæŸå°æ§æšèç©è³ªãšãã
ã®ã¡ãååŠçæ段ãå©çšããŠéç¶ååã®åå¡©åºã®
éã®çµåãç¹ç°çã«åæããã次ã«ããã®æäœã«
ããåŸãããDNAãããã¯DNAã®å解ç©ã®å€æ°
ã®åæå解ç©ã®æ··åç©ãã²ã«é»æ°æ³³åæ³ã«ããå
é¢å±éããå€æ°ã®åæå解ç©ããããã垯ç¶ã圢
æããŠåé¢å±éãããã©ãžãªã¯ãããã°ã©ã ïŒã
ã ããèŠèŠçã«ã¯èŠãããšãã§ããªãïŒãåŸãã
ãšãã§ããããã®ã©ãžãªã¯ãããã°ã©ã ãšé«æ床
ç·ãã€ã«ã ãšãäœæž©äžã«ãŠé·æééãåãããŠ
ãããšãæŸå°æ§åäœå çŽ ãååäžã«å«ãåæå解
ç©ãååšããäœçœ®ã«é¢ããç·ãã€ã«ã ã®éšåã¯
æå ããŠæœåã圢æããããã®ããã«ããŠæœåã
圢æããç·ãã€ã«ã ãçŸåããããšã«ããã©ãž
ãªã¯ãããã°ã©ã ã«å¯Ÿå¿ããå€æ°ã®åž¯ç¶åž¯åãå«
ãã¯ãããã°ã©ã ãç·ãã€ã«ã äžã«å¯èŠåãšã
ãŠçŸãããããããŠããã®å¯èŠåãããã¯ããã
ã°ã©ã ãšç¹ç°çåææ段ãšãããæŸå°æ§åäœå çŽ
ãçµåãããéç¶ååã®ç«¯éšããäžå®ã®äœçœ®é¢ä¿
ã«ããå¡©åºãé 次決å®ããããšãã§ãããã®ãã
ã«ããŠå¯Ÿè±¡ç©ã®ãã¹ãŠã®å¡©åºã®é åã決å®ããã
ãšãã§ããã
ãªããäžèšã«èŠçŽãããããµã ã»ã®ã«ããŒãæ³
ã«ã€ããŠã¯æ¬¡ã®æç®ã«è©³çŽ°ã«èšèŒãããŠããã
ã«ã€ããŠã¯æ¬¡ã®æç®ã«è©³çŽ°ã«èšèŒãããŠããã
METHODS IN ENZYMOLOGYïŒVOL.65ïŒ
PART ïŒACADEMIC PRESSïŒNEW YORK
LONDON TRONTO SYDNEY SAN
FRANCISICOïŒ1980ïŒ ãµã³ã¬ãŒã»ã¯ãŒã«ãœã³æ³ããŸãDNAã®ç¹åŸŽç
ãªæ§é ã«çç®ããDNAåæé µçŽ ãã²ã«é»æ°æ³³å
ããã³ãªãŒãã©ãžãªã°ã©ãã€ãŒãå©çšããŠDNA
ã®å¡©åºé åã決å®ããæ¹æ³ã§ããããã®ãµã³ã¬
ãŒã»ã¯ãŒã«ãœã³æ³ããã³åèšã®ãããµã ã»ã®ã«ã
ãŒãæ³ã®ç¹åŸŽããã³æäœã«ã€ããŠã®ç°¡åãªèšè¿°ã¯
次ã®æç®ã«èŠãããã
PART ïŒACADEMIC PRESSïŒNEW YORK
LONDON TRONTO SYDNEY SAN
FRANCISICOïŒ1980ïŒ ãµã³ã¬ãŒã»ã¯ãŒã«ãœã³æ³ããŸãDNAã®ç¹åŸŽç
ãªæ§é ã«çç®ããDNAåæé µçŽ ãã²ã«é»æ°æ³³å
ããã³ãªãŒãã©ãžãªã°ã©ãã€ãŒãå©çšããŠDNA
ã®å¡©åºé åã決å®ããæ¹æ³ã§ããããã®ãµã³ã¬
ãŒã»ã¯ãŒã«ãœã³æ³ããã³åèšã®ãããµã ã»ã®ã«ã
ãŒãæ³ã®ç¹åŸŽããã³æäœã«ã€ããŠã®ç°¡åãªèšè¿°ã¯
次ã®æç®ã«èŠãããã
ãéºäŒæ
å ±ãåèªã§èªãã»æè¡šãè¡ããDNA
ã®å¡©åºé å解ææ³ãäžæµŠè¬¹äžéãçŸä»£ååŠã1977
幎ïŒæå·46ã54é ïŒ(æ ª)æ±äº¬ååŠå人åïŒ ãããŸã§ã«è¿°ã¹ãããã«ãªãŒãã©ãžãªã°ã©ãã€
ãŒã¯ãæŸå°æ§æšèãä»ãããŠããçç©äœç±æ¥ã®ç©
質ã®æ··åç©ãããªãè©Šæãæ¯æäœïŒããšãã°ãé»
æ°æ³³ååé¢çšæ¯æäœããããã¯èå±€ã¯ãããã°ã©
ãã€ãŒçšæ¯æäœãªã©ïŒãçšããŠåé¢å±éããŠãªãŒ
ãã©ãžãªã°ã©ã ãåŸãã®ã¡ããã®æ¯æäœäžã§åé¢
å±éãããåç©è³ªã®äžæ¬¡å çãããã¯äºæ¬¡å çãª
äœçœ®æ å ±ãããªãŒãã©ãžãªã°ã©ã ãæ§æãã該ç©
質ã®æŸå°æ§ãå©çšããŠæ€åºæž¬å®ããããšã«ããã
ãããã®ç©è³ªã®åé¢å±éããã³åå®ãªã©ãè¡ãªã
ããã«æå¹ã«å©çšãããŠãããåŸã€ãŠããã®çš®ã®
ãªãŒãã©ãžãªã°ã©ãã€ãŒãå©çšããããšã«ããã
ããšãã°ãçäœé«ååã®æ§é 決å®ãªã©ãå¹çè¯ã
éæããããšãã§ãããããè¿å¹Žã§ã¯ãã®çš®ã®ãª
ãŒãã©ãžãªã°ã©ãã€ãŒãåºãå©çšãããŠããã
ã®å¡©åºé å解ææ³ãäžæµŠè¬¹äžéãçŸä»£ååŠã1977
幎ïŒæå·46ã54é ïŒ(æ ª)æ±äº¬ååŠå人åïŒ ãããŸã§ã«è¿°ã¹ãããã«ãªãŒãã©ãžãªã°ã©ãã€
ãŒã¯ãæŸå°æ§æšèãä»ãããŠããçç©äœç±æ¥ã®ç©
質ã®æ··åç©ãããªãè©Šæãæ¯æäœïŒããšãã°ãé»
æ°æ³³ååé¢çšæ¯æäœããããã¯èå±€ã¯ãããã°ã©
ãã€ãŒçšæ¯æäœãªã©ïŒãçšããŠåé¢å±éããŠãªãŒ
ãã©ãžãªã°ã©ã ãåŸãã®ã¡ããã®æ¯æäœäžã§åé¢
å±éãããåç©è³ªã®äžæ¬¡å çãããã¯äºæ¬¡å çãª
äœçœ®æ å ±ãããªãŒãã©ãžãªã°ã©ã ãæ§æãã該ç©
質ã®æŸå°æ§ãå©çšããŠæ€åºæž¬å®ããããšã«ããã
ãããã®ç©è³ªã®åé¢å±éããã³åå®ãªã©ãè¡ãªã
ããã«æå¹ã«å©çšãããŠãããåŸã€ãŠããã®çš®ã®
ãªãŒãã©ãžãªã°ã©ãã€ãŒãå©çšããããšã«ããã
ããšãã°ãçäœé«ååã®æ§é 決å®ãªã©ãå¹çè¯ã
éæããããšãã§ãããããè¿å¹Žã§ã¯ãã®çš®ã®ãª
ãŒãã©ãžãªã°ã©ãã€ãŒãåºãå©çšãããŠããã
ããããªããããã®ããã«æçšãªãªãŒãã©ãžãª
ã°ã©ãã€ãŒãå®éã«å©çšããå Žåã«ã¯ãããã€ã
ã®åé¡ãããã
ã°ã©ãã€ãŒãå®éã«å©çšããå Žåã«ã¯ãããã€ã
ã®åé¡ãããã
ãã®ç¬¬äžã¯ããªãŒãã©ãžãªã°ã©ã ãããªãã¡ã
æ¯æäœäžã«åé¢å±éãããç¶æ ã§ååšããæŸå°æ§
æšèç©è³ªããæ§æãããã¯ãããã°ã©ã ã«ããã
æŸå°æ§ç©è³ªã®äœçœ®ãå¯èŠåããããã«ããã®æ¯æ
äœãšé«æ床ç·ãã€ã«ã ãªã©ã®æŸå°ç·ãã€ã«ã ãš
ãäžå®æééãåãããããšã«ãã€ãŠã該ãã€ã«
ã ãæå ïŒé²å ïŒãããæäœãç ©éã§ããããã€
é·æéãå¿ èŠãšããç¹ã§ãããããªãã¡åŸæ¥ã®ãª
ãŒãã©ãžãªã°ã©ãã€ãŒã«ãããŠäžèšã®é²å æäœ
ã¯ãäœæž©ïŒããšãã°ãïŒâä»è¿ããããŠæ žé žã®å¡©
åºé å決å®ãªã©ã«ãããã²ã«ã¯ãããã°ã©ã ã®é²
å ã®å Žåã«ã¯â70ãâ90âïŒã§ããã€é·æéïŒã
ãšãã°ãæ°æ¥éïŒãããŠå®æœãããŠããããã
ã¯ããªãŒãã©ãžãªã°ã©ãã€ãŒã®æž¬å®å¯Ÿè±¡ãšãªãé
åžžã®æŸå°æ§æšèç©è³ªã¯äžè¬ã«é«ãæŸå°æ§ãæããŠ
ããªããããå åãªæå ãåŸãããã«ã¯é²å ãé·
æéããªããã°ãªããªãããšããããŠãããšãã°
宀枩ãªã©ã®æ¯èŒçé«ã枩床ã«ãŠæ¯æäœãšæŸå°ç·ã
ã€ã«ã ãšãé·æééãåãããŠãããšãæŸå°ç·ã
ã€ã«ã ã®æå æåã§ããéå¡©ãããã®æ¯æäœäžã®
åçš®ã®ç©è³ªã«ããååŠã«ããªãåãããã®ãã該
ãã€ã«ã ã«ç²ŸåºŠã®é«ãæå ç»åãåŸããã«ããã
åŸã€ãŠããã®ãããªååŠã«ããªãäœæžããããã«
é²å æäœãäœæž©äžã§è¡ãªãå¿ èŠãããããšãªã©ã®
çç±ã«ããããã®ãããªå³ããé²å æ¡ä»¶ãç·©åã
ãããã«æŸå°ç·ãã€ã«ã ã®æ床ãæŽã«é«ããããš
ãèããããããåŸæ¥ã®ãªãŒãã©ãžãªã°ã©ãã€ãŒ
ã«ãããŠæŸå°ç·ãã€ã«ã ã¯ãæ¢ã«éåžžã«é«æ床ã«
ããããã®ãçšããããŠãããåŸãããç»åã®é®®
æããèæ ®ãããšãæŸå°ç·ãã€ã«ã ã®é£èºçãªé«
æ床åã¯å°é£ã§ããã
æ¯æäœäžã«åé¢å±éãããç¶æ ã§ååšããæŸå°æ§
æšèç©è³ªããæ§æãããã¯ãããã°ã©ã ã«ããã
æŸå°æ§ç©è³ªã®äœçœ®ãå¯èŠåããããã«ããã®æ¯æ
äœãšé«æ床ç·ãã€ã«ã ãªã©ã®æŸå°ç·ãã€ã«ã ãš
ãäžå®æééãåãããããšã«ãã€ãŠã該ãã€ã«
ã ãæå ïŒé²å ïŒãããæäœãç ©éã§ããããã€
é·æéãå¿ èŠãšããç¹ã§ãããããªãã¡åŸæ¥ã®ãª
ãŒãã©ãžãªã°ã©ãã€ãŒã«ãããŠäžèšã®é²å æäœ
ã¯ãäœæž©ïŒããšãã°ãïŒâä»è¿ããããŠæ žé žã®å¡©
åºé å決å®ãªã©ã«ãããã²ã«ã¯ãããã°ã©ã ã®é²
å ã®å Žåã«ã¯â70ãâ90âïŒã§ããã€é·æéïŒã
ãšãã°ãæ°æ¥éïŒãããŠå®æœãããŠããããã
ã¯ããªãŒãã©ãžãªã°ã©ãã€ãŒã®æž¬å®å¯Ÿè±¡ãšãªãé
åžžã®æŸå°æ§æšèç©è³ªã¯äžè¬ã«é«ãæŸå°æ§ãæããŠ
ããªããããå åãªæå ãåŸãããã«ã¯é²å ãé·
æéããªããã°ãªããªãããšããããŠãããšãã°
宀枩ãªã©ã®æ¯èŒçé«ã枩床ã«ãŠæ¯æäœãšæŸå°ç·ã
ã€ã«ã ãšãé·æééãåãããŠãããšãæŸå°ç·ã
ã€ã«ã ã®æå æåã§ããéå¡©ãããã®æ¯æäœäžã®
åçš®ã®ç©è³ªã«ããååŠã«ããªãåãããã®ãã該
ãã€ã«ã ã«ç²ŸåºŠã®é«ãæå ç»åãåŸããã«ããã
åŸã€ãŠããã®ãããªååŠã«ããªãäœæžããããã«
é²å æäœãäœæž©äžã§è¡ãªãå¿ èŠãããããšãªã©ã®
çç±ã«ããããã®ãããªå³ããé²å æ¡ä»¶ãç·©åã
ãããã«æŸå°ç·ãã€ã«ã ã®æ床ãæŽã«é«ããããš
ãèããããããåŸæ¥ã®ãªãŒãã©ãžãªã°ã©ãã€ãŒ
ã«ãããŠæŸå°ç·ãã€ã«ã ã¯ãæ¢ã«éåžžã«é«æ床ã«
ããããã®ãçšããããŠãããåŸãããç»åã®é®®
æããèæ ®ãããšãæŸå°ç·ãã€ã«ã ã®é£èºçãªé«
æ床åã¯å°é£ã§ããã
ãŸããæŸå°ç·ãã€ã«ã ã®æå
æåã®éå¡©ã¯ååŠ
çåºæ¿ã®ã¿ã§ãªããç©ççãªåºæ¿ã«ã圱é¿ããã
ããæ¬ ç¹ããããããããªãŒãã©ãžãªã°ã©ãã€ãŒ
ã®æäœãå°é£ã«ãããã€ãã®ç²ŸåºŠãäœäžãããå
å ãšãªããããªãã¡ããªãŒãã©ãžãªã°ã©ãã€ãŒã«
ã§ã¯äžè¬ã«æŸå°ç·ãã€ã«ã ãæ¯æäœãšæ¥è§Šããç¶
æ ãšããŠé²å æäœãè¡ãªãå¿ èŠããããããæŸå°
ç·ãã€ã«ã ã®ç§»åãèšçœ®ãªã©ã®äœæ¥ã¯æŸå°ç·ãã€
ã«ã ã裞ã®ç¶æ ã«ããŠè¡ãªãããšãå€ããåŸã€
ãŠããã®ãããªäœæ¥ã®éã«ãæŸå°ç·ãã€ã«ã ãæ
äœæ åœè ã®æãããã¯æ©åšãªã©ã«æ¥è§Šããæ©äŒã
å¢å ãããã®æ¥è§Šãªã©ã«èµ·å ããç©ççå§åãªã©
ã«ãã€ãŠæŸå°ç·ãã€ã«ã ã¯ç©ççã«ããªçŸè±¡ãèµ·
ãåŸåãããããã®ç¹ããªãŒãã©ãžãªã°ã©ãã€ãŒ
ã®ç²ŸåºŠãäœäžãããåå ãšãªãããããŠããã®ã
ããªæŸå°ç·ãã€ã«ã ã®ç©ççã«ããªã®çºçãåé¿
ããããã«ã¯ããã®åæ±ãäœæ¥ã«ãããŠé«åºŠã®ç
ç·Žãšæ³šæãšãå¿ èŠãšãããªãŒãã©ãžãªã°ã©ãã€ãŒ
ã®æäœãããã«è€éã«ããçµæãšãªãã
çåºæ¿ã®ã¿ã§ãªããç©ççãªåºæ¿ã«ã圱é¿ããã
ããæ¬ ç¹ããããããããªãŒãã©ãžãªã°ã©ãã€ãŒ
ã®æäœãå°é£ã«ãããã€ãã®ç²ŸåºŠãäœäžãããå
å ãšãªããããªãã¡ããªãŒãã©ãžãªã°ã©ãã€ãŒã«
ã§ã¯äžè¬ã«æŸå°ç·ãã€ã«ã ãæ¯æäœãšæ¥è§Šããç¶
æ ãšããŠé²å æäœãè¡ãªãå¿ èŠããããããæŸå°
ç·ãã€ã«ã ã®ç§»åãèšçœ®ãªã©ã®äœæ¥ã¯æŸå°ç·ãã€
ã«ã ã裞ã®ç¶æ ã«ããŠè¡ãªãããšãå€ããåŸã€
ãŠããã®ãããªäœæ¥ã®éã«ãæŸå°ç·ãã€ã«ã ãæ
äœæ åœè ã®æãããã¯æ©åšãªã©ã«æ¥è§Šããæ©äŒã
å¢å ãããã®æ¥è§Šãªã©ã«èµ·å ããç©ççå§åãªã©
ã«ãã€ãŠæŸå°ç·ãã€ã«ã ã¯ç©ççã«ããªçŸè±¡ãèµ·
ãåŸåãããããã®ç¹ããªãŒãã©ãžãªã°ã©ãã€ãŒ
ã®ç²ŸåºŠãäœäžãããåå ãšãªãããããŠããã®ã
ããªæŸå°ç·ãã€ã«ã ã®ç©ççã«ããªã®çºçãåé¿
ããããã«ã¯ããã®åæ±ãäœæ¥ã«ãããŠé«åºŠã®ç
ç·Žãšæ³šæãšãå¿ èŠãšãããªãŒãã©ãžãªã°ã©ãã€ãŒ
ã®æäœãããã«è€éã«ããçµæãšãªãã
ãŸãããã«ãåŸæ¥ã®ãªãŒãã©ãžãªã°ã©ãã€ãŒã§
ã¯äžèšã®ããã«é·æéã®é²å æäœãè¡ãªãããã
ããæŸå°æ§æšèç©è³ªä»¥å€ã«è©Šæäžã«å«ãŸããèªç¶
æŸå°èœããŸãæŸå°ç·ãã€ã«ã ã®é²å ã«é¢äžããåŸ
ãããæŸå°æ§æšèç©è³ªã®äœçœ®æ å ±ã®ç²ŸåºŠãäœäžã
ãããšã®åé¡ãããããã®ãããªèªç¶æŸå°èœã«ã
ã劚害ãé€ãããã«ãããšãã°ãå¯Ÿç §è©Šæãçšã
ã䞊è¡å®éšã®å®æœãé²å æéã®é©æ£åãªã©ãå³ã
ããŠãããã䞊è¡å®éšã®å®æœã«ããå®éšåæ°ã®å¢
倧ã奜é©ãªé²å æéã®æ±ºå®ãè¡ãªãããã®äºåå®
éšã®å¿ èŠæ§ãªã©ã«ããããã®æäœå šäœãç ©éã«ãª
ããšã®æ¬ ç¹ãããã
ã¯äžèšã®ããã«é·æéã®é²å æäœãè¡ãªãããã
ããæŸå°æ§æšèç©è³ªä»¥å€ã«è©Šæäžã«å«ãŸããèªç¶
æŸå°èœããŸãæŸå°ç·ãã€ã«ã ã®é²å ã«é¢äžããåŸ
ãããæŸå°æ§æšèç©è³ªã®äœçœ®æ å ±ã®ç²ŸåºŠãäœäžã
ãããšã®åé¡ãããããã®ãããªèªç¶æŸå°èœã«ã
ã劚害ãé€ãããã«ãããšãã°ãå¯Ÿç §è©Šæãçšã
ã䞊è¡å®éšã®å®æœãé²å æéã®é©æ£åãªã©ãå³ã
ããŠãããã䞊è¡å®éšã®å®æœã«ããå®éšåæ°ã®å¢
倧ã奜é©ãªé²å æéã®æ±ºå®ãè¡ãªãããã®äºåå®
éšã®å¿ èŠæ§ãªã©ã«ããããã®æäœå šäœãç ©éã«ãª
ããšã®æ¬ ç¹ãããã
æ¬çºæè
ã¯ãåŸæ¥ã®ãªãŒãã©ãžãªã°ã©ãã€ãŒã«
ééããäžèšã®ãããªåé¡ç¹ã®è§£æ±ºãç®çãšããŠ
éæç 究ãè¡ãªã€ãçµæãåé¢å±éçšæ¯æäœäžã«
圢æããããªãŒãã©ãžãªã°ã©ã ãšçµåãããŠçšã
ãæå ææãšããŠæŸå°ç·ãã€ã«ã ã®ä»£ãã«ãèŒå°œ
æ§èå äœãçµåå€äžã«åæ£ããŠãªãèå äœå±€ãæ
ããèç©æ§èå äœã·ãŒããçšããããšã«ãããå
èšã®åé¡ç¹ã®è§£æ±ºãããã¯æ¬ ç¹ã®äœæžãå®çŸãã
ããšãèŠåºããæ¬çºæã«å°éããã
ééããäžèšã®ãããªåé¡ç¹ã®è§£æ±ºãç®çãšããŠ
éæç 究ãè¡ãªã€ãçµæãåé¢å±éçšæ¯æäœäžã«
圢æããããªãŒãã©ãžãªã°ã©ã ãšçµåãããŠçšã
ãæå ææãšããŠæŸå°ç·ãã€ã«ã ã®ä»£ãã«ãèŒå°œ
æ§èå äœãçµåå€äžã«åæ£ããŠãªãèå äœå±€ãæ
ããèç©æ§èå äœã·ãŒããçšããããšã«ãããå
èšã®åé¡ç¹ã®è§£æ±ºãããã¯æ¬ ç¹ã®äœæžãå®çŸãã
ããšãèŠåºããæ¬çºæã«å°éããã
ããªãã¡ããªãŒãã©ãžãªã°ã©ã ã瀺ãåé¢å±é
çšæ¯æäœäžã®æŸå°æ§æšèç©è³ªã®äœçœ®æ å ±ãåŸãã
ãã«çšããæå ææãšããŠãèŒå°œæ§èå äœãçµå
å€äžã«åæ£ããŠãªãèå äœå±€ãæããèç©æ§èå
äœã·ãŒããçšããå Žåã«ã¯ãé²å æéã®å€§å¹ ãªç
çž®åãå®çŸããã®ã¿ã§ãªããç°å¢æž©åºŠãããã¯ã
ã®ä»è¿ã®æž©åºŠãšãã枩床æ¡ä»¶ã§ãã€ãŠããåŸãã
ãæŸå°æ§æšèç©è³ªã®äœçœ®æ å ±ã®ç²ŸåºŠãäœäžããã
ããšãªããé²å ãè¡ãªãããšãã§ããããšãå€æ
ããããã®ç¹ã¯åŸæ¥ã®ãããŠå·åŽäžã«å®æœãããŠ
ãããªãŒãã©ãžãªã°ã©ãã€ãŒã«ãããé²å æäœã
èããç°¡ç¥åãããã®ã§ããããŸãé²å æéã®å€§
å¹ ãªççž®åãå®çŸããããšã«ããããªãŒãã©ãžãª
ã°ã©ãã€ãŒã®æäœå šäœãçæéã§å¹çè¯ãå®æœã§
ããããšã«ãªãããã®ç¹ã«ãããŠãå®çšäžéåžžã«
æå©ãšãªãã
çšæ¯æäœäžã®æŸå°æ§æšèç©è³ªã®äœçœ®æ å ±ãåŸãã
ãã«çšããæå ææãšããŠãèŒå°œæ§èå äœãçµå
å€äžã«åæ£ããŠãªãèå äœå±€ãæããèç©æ§èå
äœã·ãŒããçšããå Žåã«ã¯ãé²å æéã®å€§å¹ ãªç
çž®åãå®çŸããã®ã¿ã§ãªããç°å¢æž©åºŠãããã¯ã
ã®ä»è¿ã®æž©åºŠãšãã枩床æ¡ä»¶ã§ãã€ãŠããåŸãã
ãæŸå°æ§æšèç©è³ªã®äœçœ®æ å ±ã®ç²ŸåºŠãäœäžããã
ããšãªããé²å ãè¡ãªãããšãã§ããããšãå€æ
ããããã®ç¹ã¯åŸæ¥ã®ãããŠå·åŽäžã«å®æœãããŠ
ãããªãŒãã©ãžãªã°ã©ãã€ãŒã«ãããé²å æäœã
èããç°¡ç¥åãããã®ã§ããããŸãé²å æéã®å€§
å¹ ãªççž®åãå®çŸããããšã«ããããªãŒãã©ãžãª
ã°ã©ãã€ãŒã®æäœå šäœãçæéã§å¹çè¯ãå®æœã§
ããããšã«ãªãããã®ç¹ã«ãããŠãå®çšäžéåžžã«
æå©ãšãªãã
ããã«ããªãŒãã©ãžãªã°ã©ãã€ãŒã®æå
ææãš
ããŠäžèšã®èç©æ§èå äœã·ãŒããå©çšããããšã«
ãããåŸæ¥æŸå°ç·ãã€ã«ã ã®äœ¿çšã«ãããŠå€§ããª
åé¡ãšãªã€ãŠããååŠã«ããªããã³ç©çã«ããªã
å®è³ªçã«çºçããªããªãç¹ãããªãŒãã©ãžãªã°ã©
ãã€ãŒã®ç²ŸåºŠã®åäžããã³äœæ¥æ§ã«ãããŠéåžžã«
æå©ã«äœçšããã
ããŠäžèšã®èç©æ§èå äœã·ãŒããå©çšããããšã«
ãããåŸæ¥æŸå°ç·ãã€ã«ã ã®äœ¿çšã«ãããŠå€§ããª
åé¡ãšãªã€ãŠããååŠã«ããªããã³ç©çã«ããªã
å®è³ªçã«çºçããªããªãç¹ãããªãŒãã©ãžãªã°ã©
ãã€ãŒã®ç²ŸåºŠã®åäžããã³äœæ¥æ§ã«ãããŠéåžžã«
æå©ã«äœçšããã
ãŸããæå
ææãšããŠèç©æ§èå
äœã·ãŒãã䜿
çšããå Žåã«ã¯ãè©Šæããèç©æ§èå äœã·ãŒãã«
転åãããæŸå°æ§æšèç©è³ªã®äœçœ®æ å ±ãåŸããã
ã«ã¯ãç¹ã«ç»ååããå¿ èŠã¯ãªãããã®èç©æ§è
å äœã·ãŒããã¬ãŒã¶ãŒãªã©ã®é»ç£æ³¢ã§èµ°æ»ããã
ãšã«ããäžèšã®äœçœ®æ å ±ãèªã¿åºãããã®äœçœ®æ
å ±ãç»åãèšå·ãããã¯æ°å€ããããã¯ãããã®
çµåãããªã©ã®ä»»æãªåœ¢æ ã«å€ããŠåãåºãããš
ãå¯èœãšãªããããã«ãäžèšã®äœçœ®æ å ±ãé»æ°ç
æ段ãªã©ãå©çšããŠæŽã«å å·¥ããããšã«ãããæ
æã®åçš®ã®åœ¢æ ã§å¿ èŠãªæ å ±ãå ¥æããããšãå¯
èœã§ããã
çšããå Žåã«ã¯ãè©Šæããèç©æ§èå äœã·ãŒãã«
転åãããæŸå°æ§æšèç©è³ªã®äœçœ®æ å ±ãåŸããã
ã«ã¯ãç¹ã«ç»ååããå¿ èŠã¯ãªãããã®èç©æ§è
å äœã·ãŒããã¬ãŒã¶ãŒãªã©ã®é»ç£æ³¢ã§èµ°æ»ããã
ãšã«ããäžèšã®äœçœ®æ å ±ãèªã¿åºãããã®äœçœ®æ
å ±ãç»åãèšå·ãããã¯æ°å€ããããã¯ãããã®
çµåãããªã©ã®ä»»æãªåœ¢æ ã«å€ããŠåãåºãããš
ãå¯èœãšãªããããã«ãäžèšã®äœçœ®æ å ±ãé»æ°ç
æ段ãªã©ãå©çšããŠæŽã«å å·¥ããããšã«ãããæ
æã®åçš®ã®åœ¢æ ã§å¿ èŠãªæ å ±ãå ¥æããããšãå¯
èœã§ããã
ããã«ãŸããè©Šæäžã«å«ãŸããŠããèªç¶æŸå°èœ
ãªã©ã«èµ·å ãã粟床ã劚害ãããããªåœ±é¿ã¯ãè
ç©æ§èå äœã·ãŒãã«èç©ãããŠããäœçœ®æ å ±ãé»
æ°çã«åŠçããããšã«ãã容æã«äœæžãããã¯æ¶
å»ããããšãå¯èœãšãªãã
ãªã©ã«èµ·å ãã粟床ã劚害ãããããªåœ±é¿ã¯ãè
ç©æ§èå äœã·ãŒãã«èç©ãããŠããäœçœ®æ å ±ãé»
æ°çã«åŠçããããšã«ãã容æã«äœæžãããã¯æ¶
å»ããããšãå¯èœãšãªãã
åŸã€ãŠãæ¬çºæã¯ãæŸå°æ§æšèãä»ãããçç©
äœç±æ¥ã®ç©è³ªãåé¢å±éããããã®æ¯æäœããã
ã³èŒå°œæ§èå äœãçµåå€äžã«åæ£ããŠãªãèå äœ
å±€ãæããèç©æ§èå äœã·ãŒããå«ããªãŒãã©ãž
ãªã°ã©ãã€ãŒçšæž¬å®ããããäŸçµŠãããã®ã§ã
ãã
äœç±æ¥ã®ç©è³ªãåé¢å±éããããã®æ¯æäœããã
ã³èŒå°œæ§èå äœãçµåå€äžã«åæ£ããŠãªãèå äœ
å±€ãæããèç©æ§èå äœã·ãŒããå«ããªãŒãã©ãž
ãªã°ã©ãã€ãŒçšæž¬å®ããããäŸçµŠãããã®ã§ã
ãã
æ¬çºæã®ãªãŒãã©ãžãªã°ã©ãã€ãŒçšæž¬å®ããã
ã«ãããŠäžæ¹ã®æ§æéšæãšããŠäœ¿çšããèç©æ§è
å äœã·ãŒãã¯æŸå°ç·åå€æããã«ãšãåŒã°ããã®
ã§ããããã®äŸã¯ãããšãã°ãç¹éæ55â12145
å·å ¬å ±ãªã©ã«èšèŒãããŠãããäžè¬çãªæ§æãšã
ãŠã¯æ¢ã«å ¬ç¥ã§ããã
ã«ãããŠäžæ¹ã®æ§æéšæãšããŠäœ¿çšããèç©æ§è
å äœã·ãŒãã¯æŸå°ç·åå€æããã«ãšãåŒã°ããã®
ã§ããããã®äŸã¯ãããšãã°ãç¹éæ55â12145
å·å ¬å ±ãªã©ã«èšèŒãããŠãããäžè¬çãªæ§æãšã
ãŠã¯æ¢ã«å ¬ç¥ã§ããã
ããªãã¡ãèç©æ§èå
äœã·ãŒãã¯è¢«åäœãéé
ããæŸå°ç·ãšãã«ã®ãŒããããã¯è¢«æ€äœããçºã
ãããæŸå°ç·ãšãã«ã®ãŒã該ããã«ã®èŒå°œæ§èå
äœã«åžåããããã®ã®ã¡ã«èŒå°œæ§èå äœãå¯èŠå
ç·ããã³èµ€å€ç·ãªã©ã®é»ç£æ³¢ïŒå±èµ·å ïŒãçšããŠ
æç³»åçã«å±èµ·ããããšã«ãããèŒå°œæ§èå äœäž
ã«èç©ãããŠããæŸå°ç·ãšãã«ã®ãŒãèå ãšããŠ
æŸåºããããã®èå ãå é»çã«èªã¿åã€ãŠé»æ°ä¿¡
å·ãåŸããã®é»æ°ä¿¡å·ãæå ãã€ã«ã ãªã©ã®èšé²
ææãCRTãªã©ã®è¡šç€ºè£ 眮äžã«å¯èŠç»åãšããŠ
åçãããããããã¯æ°å€åãããã¯èšå·åãã
äœçœ®æ å ±ãªã©ãšããŠè¡šãããã®ã§ããã
ããæŸå°ç·ãšãã«ã®ãŒããããã¯è¢«æ€äœããçºã
ãããæŸå°ç·ãšãã«ã®ãŒã該ããã«ã®èŒå°œæ§èå
äœã«åžåããããã®ã®ã¡ã«èŒå°œæ§èå äœãå¯èŠå
ç·ããã³èµ€å€ç·ãªã©ã®é»ç£æ³¢ïŒå±èµ·å ïŒãçšããŠ
æç³»åçã«å±èµ·ããããšã«ãããèŒå°œæ§èå äœäž
ã«èç©ãããŠããæŸå°ç·ãšãã«ã®ãŒãèå ãšããŠ
æŸåºããããã®èå ãå é»çã«èªã¿åã€ãŠé»æ°ä¿¡
å·ãåŸããã®é»æ°ä¿¡å·ãæå ãã€ã«ã ãªã©ã®èšé²
ææãCRTãªã©ã®è¡šç€ºè£ 眮äžã«å¯èŠç»åãšããŠ
åçãããããããã¯æ°å€åãããã¯èšå·åãã
äœçœ®æ å ±ãªã©ãšããŠè¡šãããã®ã§ããã
以äžã«ãæ¬çºæã®ãªãŒãã©ãžãªã°ã©ãã€ãŒçšæž¬
å®ãããã«ãããŠå¥œé©ã«äœ¿çšãããèç©æ§èå äœ
ã·ãŒãã«ã€ããŠç°¡åã«èª¬æããã
å®ãããã«ãããŠå¥œé©ã«äœ¿çšãããèç©æ§èå äœ
ã·ãŒãã«ã€ããŠç°¡åã«èª¬æããã
äžèšã®èç©æ§èå
äœã·ãŒãã¯ãåºæ¬æ§é ãšããŠ
æ¯æäœãšããã®çé¢ã«èšããããèå äœå±€ãšãã
ãªããã®ã§ããããã ãããã®èå äœå±€ã®æ¯æäœ
ãšã¯å察åŽã®è¡šé¢ïŒæ¯æäœã«é¢ããŠããªãåŽã®è¡š
é¢ïŒã«ã¯äžè¬ã«ãéæãªä¿è·èãèšããããèå
äœå±€ãååŠçãªå€è³ªãããã¯ç©ççãªè¡æããä¿
è·ããŠããã
æ¯æäœãšããã®çé¢ã«èšããããèå äœå±€ãšãã
ãªããã®ã§ããããã ãããã®èå äœå±€ã®æ¯æäœ
ãšã¯å察åŽã®è¡šé¢ïŒæ¯æäœã«é¢ããŠããªãåŽã®è¡š
é¢ïŒã«ã¯äžè¬ã«ãéæãªä¿è·èãèšããããèå
äœå±€ãååŠçãªå€è³ªãããã¯ç©ççãªè¡æããä¿
è·ããŠããã
èå
äœå±€ã¯ãèŒå°œæ§èå
äœãšããããåæ£ç¶æ
ã§å«ææ¯æããçµåå€ãšãããªããã®ã§ãããã
ã®èŒå°œæ§èå äœã¯ãæŸå°ç·ãåžåããã®ã¡ãå¯èŠ
å ç·ããã³èµ€å€ç·ãªã©ã®é»ç£æ³¢ïŒå±èµ·å ïŒã®ç §å°
ãåãããšçºå ïŒèŒå°œçºå ïŒã瀺ãæ§è³ªãæãã
ãã®ã§ãããåŸã€ãŠãããšãã°ãæŸå°æ§æšèç©è³ª
ãå«ãè©Šæãªã©ã®ãããªè¢«æ€äœããçºããããæŸ
å°ç·ã¯ããã®æŸå°ç·éã«æ¯äŸããŠèç©æ§èå äœã·
ãŒãã®èå äœå±€ã«åžåãããèç©æ§èå äœã·ãŒã
äžã«ã¯è¢«æ€äœã®æŸå°ç·åãæŸå°ç·ãšãã«ã®ãŒã®è
ç©åãšããŠåœ¢æãããããã®èç©åã¯ãå¯èŠå ç·
ããã³èµ€å€ç·ãªã©ã®é»ç£æ³¢ïŒå±èµ·å ïŒã§å±èµ·ãã
ããšã«ãããèŒå°œçºå ïŒèå ïŒãšããŠæŸå°ããã
ããšãã§ãããã®èŒå°œçºå ãå é»çã«èªã¿åã€ãŠ
é»æ°ä¿¡å·ã«å€æããããšã«ãããæŸå°ç·ãšãã«ã®
ãŒã®èç©åãå¯èŠç»åããããã¯æŸå°æ§ïŒæšèïŒ
ç©è³ªã®äœçœ®æ å ±ã瀺ãæ°å€ãèšå·ãªã©ã«å€æãã
ããšãå¯èœãšãªãã
ã§å«ææ¯æããçµåå€ãšãããªããã®ã§ãããã
ã®èŒå°œæ§èå äœã¯ãæŸå°ç·ãåžåããã®ã¡ãå¯èŠ
å ç·ããã³èµ€å€ç·ãªã©ã®é»ç£æ³¢ïŒå±èµ·å ïŒã®ç §å°
ãåãããšçºå ïŒèŒå°œçºå ïŒã瀺ãæ§è³ªãæãã
ãã®ã§ãããåŸã€ãŠãããšãã°ãæŸå°æ§æšèç©è³ª
ãå«ãè©Šæãªã©ã®ãããªè¢«æ€äœããçºããããæŸ
å°ç·ã¯ããã®æŸå°ç·éã«æ¯äŸããŠèç©æ§èå äœã·
ãŒãã®èå äœå±€ã«åžåãããèç©æ§èå äœã·ãŒã
äžã«ã¯è¢«æ€äœã®æŸå°ç·åãæŸå°ç·ãšãã«ã®ãŒã®è
ç©åãšããŠåœ¢æãããããã®èç©åã¯ãå¯èŠå ç·
ããã³èµ€å€ç·ãªã©ã®é»ç£æ³¢ïŒå±èµ·å ïŒã§å±èµ·ãã
ããšã«ãããèŒå°œçºå ïŒèå ïŒãšããŠæŸå°ããã
ããšãã§ãããã®èŒå°œçºå ãå é»çã«èªã¿åã€ãŠ
é»æ°ä¿¡å·ã«å€æããããšã«ãããæŸå°ç·ãšãã«ã®
ãŒã®èç©åãå¯èŠç»åããããã¯æŸå°æ§ïŒæšèïŒ
ç©è³ªã®äœçœ®æ å ±ã瀺ãæ°å€ãèšå·ãªã©ã«å€æãã
ããšãå¯èœãšãªãã
æ¬çºæã«ãããŠäœ¿çšããèç©æ§èå
äœã·ãŒãã®
æ¯æäœã¯ãåŸæ¥ã®æŸå°ç·åçæ³ã«ãããå¢æçŽã®
æ¯æäœãšããŠçšããããŠããåçš®ã®ææããä»»æ
ã«éžã¶ããšãã§ããããã®ãããªææã®äŸãšããŠ
ã¯ãã»ã«ããŒã¹ã¢ã»ããŒããããªãšã¹ãã«ãããª
ãšãã¬ã³ãã¬ãã¿ã¬ãŒããããªã¢ãããããªã€ã
ããããªã¢ã»ããŒããããªã«ãŒãããŒããªã©ã®ã
ã©ã¹ããã¯ç©è³ªã®ãã€ã«ã ãã¢ã«ãããŠã ç®ãã¢
ã«ãããŠã åéç®ãªã©ã®éå±ã·ãŒããéåžžã®çŽã
ãã©ã€ã¿çŽãã¬ãžã³ã³ãŒãçŽãäºé žåãã¿ã³ãªã©
ã®é¡æãå«æãããã°ã¡ã³ãçŽãããªããã«ã¢ã«
ã³ãŒã«ãªã©ããµã€ãžã³ã°ããçŽãªã©ãæããããš
ãã§ããããã ããèç©æ§èå äœã·ãŒãã®æ å ±èš
é²ææãšããŠã®ç¹æ§ããã³åæ±ããªã©ãèæ ®ãã
å Žåãæ¬çºæã«ãããŠç¹ã«å¥œãŸããæ¯æäœã®ææ
ã¯ãã©ã¹ããã¯ãã€ã«ã ã§ããããã®ãã©ã¹ãã
ã¯ãã€ã«ã ã«ã¯ã«ãŒãã³ãã©ãã¯ãªã©ã®å åžåæ§
ç©è³ªãç·Žã蟌ãŸããŠããŠãããããããã¯äºé žå
ãã¿ã³ãªã©ã®å åå°æ§ç©è³ªãç·Žã蟌ãŸããŠããŠã
ãããåè ã¯é«é®®é床ã¿ã€ãã®èç©æ§èå äœã·ãŒ
ãã«é©ããæ¯æäœã§ãããåŸè ã¯é«æ床ã¿ã€ãã®
èç©æ§èå äœã·ãŒãã«é©ããæ¯æäœã§ããã
æ¯æäœã¯ãåŸæ¥ã®æŸå°ç·åçæ³ã«ãããå¢æçŽã®
æ¯æäœãšããŠçšããããŠããåçš®ã®ææããä»»æ
ã«éžã¶ããšãã§ããããã®ãããªææã®äŸãšããŠ
ã¯ãã»ã«ããŒã¹ã¢ã»ããŒããããªãšã¹ãã«ãããª
ãšãã¬ã³ãã¬ãã¿ã¬ãŒããããªã¢ãããããªã€ã
ããããªã¢ã»ããŒããããªã«ãŒãããŒããªã©ã®ã
ã©ã¹ããã¯ç©è³ªã®ãã€ã«ã ãã¢ã«ãããŠã ç®ãã¢
ã«ãããŠã åéç®ãªã©ã®éå±ã·ãŒããéåžžã®çŽã
ãã©ã€ã¿çŽãã¬ãžã³ã³ãŒãçŽãäºé žåãã¿ã³ãªã©
ã®é¡æãå«æãããã°ã¡ã³ãçŽãããªããã«ã¢ã«
ã³ãŒã«ãªã©ããµã€ãžã³ã°ããçŽãªã©ãæããããš
ãã§ããããã ããèç©æ§èå äœã·ãŒãã®æ å ±èš
é²ææãšããŠã®ç¹æ§ããã³åæ±ããªã©ãèæ ®ãã
å Žåãæ¬çºæã«ãããŠç¹ã«å¥œãŸããæ¯æäœã®ææ
ã¯ãã©ã¹ããã¯ãã€ã«ã ã§ããããã®ãã©ã¹ãã
ã¯ãã€ã«ã ã«ã¯ã«ãŒãã³ãã©ãã¯ãªã©ã®å åžåæ§
ç©è³ªãç·Žã蟌ãŸããŠããŠãããããããã¯äºé žå
ãã¿ã³ãªã©ã®å åå°æ§ç©è³ªãç·Žã蟌ãŸããŠããŠã
ãããåè ã¯é«é®®é床ã¿ã€ãã®èç©æ§èå äœã·ãŒ
ãã«é©ããæ¯æäœã§ãããåŸè ã¯é«æ床ã¿ã€ãã®
èç©æ§èå äœã·ãŒãã«é©ããæ¯æäœã§ããã
å
¬ç¥ã®èç©æ§èå
äœã·ãŒãã«ãããŠãæ¯æäœãš
èå äœå±€ã®çµåã匷åããããããããã¯èç©æ§
èå äœã·ãŒããšããŠã®æ床ãããã¯ç»è³ªãåäžã
ããããã«ãèå äœå±€ãèšããããåŽã®æ¯æäœè¡š
é¢ã«ãŒã©ãã³ãªã©ã®é«ååç©è³ªãå¡åžããŠæ¥çæ§
ä»äžå±€ãšãããããããã¯äºé žåãã¿ã³ãªã©ã®å
åå°æ§ç©è³ªãããªãå åå°å±€ããããã¯ã«ãŒãã³
ãã©ãã¯ãªã©ã®å åžåæ§ç©è³ªãããªãå åžåå±€ã
èšããããšãè¡ãªãããŠãããæ¬çºæã«ãããŠçš
ããããæ¯æäœã«ã€ããŠãããããã®åçš®ã®å±€ã
èšããããšãã§ãããããã®æ§æã¯ææã®èç©æ§
èå äœã·ãŒãã®ç®çãçšéãªã©ã«å¿ããŠä»»æã«éž
æããããšãã§ããã
èå äœå±€ã®çµåã匷åããããããããã¯èç©æ§
èå äœã·ãŒããšããŠã®æ床ãããã¯ç»è³ªãåäžã
ããããã«ãèå äœå±€ãèšããããåŽã®æ¯æäœè¡š
é¢ã«ãŒã©ãã³ãªã©ã®é«ååç©è³ªãå¡åžããŠæ¥çæ§
ä»äžå±€ãšãããããããã¯äºé žåãã¿ã³ãªã©ã®å
åå°æ§ç©è³ªãããªãå åå°å±€ããããã¯ã«ãŒãã³
ãã©ãã¯ãªã©ã®å åžåæ§ç©è³ªãããªãå åžåå±€ã
èšããããšãè¡ãªãããŠãããæ¬çºæã«ãããŠçš
ããããæ¯æäœã«ã€ããŠãããããã®åçš®ã®å±€ã
èšããããšãã§ãããããã®æ§æã¯ææã®èç©æ§
èå äœã·ãŒãã®ç®çãçšéãªã©ã«å¿ããŠä»»æã«éž
æããããšãã§ããã
ããã«ãæ¬åºé¡äººã«ããç¹é¡æ57â82431å·æ
现æžã«é瀺ãããŠããããã«ãåŸãããç»åã®é®®
é床ãåäžãããç®çã§ãæ¯æäœã®èå äœå±€åŽã®
è¡šé¢ïŒæ¯æäœã®èå äœå±€åŽã®è¡šé¢ã«æ¥çæ§ä»äž
å±€ãå åå°å±€ãå åžåå±€ããããã¯éå±ç®ãªã©ã
èšããããŠããå Žåã«ã¯ããã®è¡šé¢ãæå³ããïŒ
ã«ã¯ãå¹åžã圢æãããŠããŠãããã
现æžã«é瀺ãããŠããããã«ãåŸãããç»åã®é®®
é床ãåäžãããç®çã§ãæ¯æäœã®èå äœå±€åŽã®
è¡šé¢ïŒæ¯æäœã®èå äœå±€åŽã®è¡šé¢ã«æ¥çæ§ä»äž
å±€ãå åå°å±€ãå åžåå±€ããããã¯éå±ç®ãªã©ã
èšããããŠããå Žåã«ã¯ããã®è¡šé¢ãæå³ããïŒ
ã«ã¯ãå¹åžã圢æãããŠããŠãããã
æ¯æäœã®äžã«ã¯ãåèšã®ããã«èå
äœå±€ã圢æ
ãããŠãããèå äœå±€ã¯ãåºæ¬çã«ã¯ç²åç¶ã®èŒ
å°œæ§èå äœãåæ£ç¶æ ã§å«ææ¯æããçµåå€ãã
ãªãå±€ã§ããã
ãããŠãããèå äœå±€ã¯ãåºæ¬çã«ã¯ç²åç¶ã®èŒ
å°œæ§èå äœãåæ£ç¶æ ã§å«ææ¯æããçµåå€ãã
ãªãå±€ã§ããã
èŒå°œæ§èå
äœã¯ãå
ã«è¿°ã¹ãããã«æŸå°ç·ãç
§
å°ããåŸãå±èµ·å ãç §å°ãããšèŒå°œçºå ã瀺ãè
å äœã§ããããå®çšçãªé¢ããã¯æ³¢é·ã400ã
800nmã®ç¯å²ã«ããå±èµ·å ã«ãã€ãŠ300ã500nm
ã®æ³¢é·ç¯å²ã®èŒå°œçºå ã瀺ãèå äœã§ããããšã
æãŸãããæ¬çºæã«ãããŠå©çšãããèç©æ§èå
äœã·ãŒãã«çšããããèŒå°œæ§èå äœãšããŠã¯ãäº
䟡ã®ãŠãŒãããŠã ã«ãã賊掻ãããŠããã¢ã«ã«ãª
åé¡éå±åŒåããã²ã³åç©ç³»èå äœã§ããããšã
奜ãŸããããããã«éå®ããããã®ã§ã¯ãªãã
å°ããåŸãå±èµ·å ãç §å°ãããšèŒå°œçºå ã瀺ãè
å äœã§ããããå®çšçãªé¢ããã¯æ³¢é·ã400ã
800nmã®ç¯å²ã«ããå±èµ·å ã«ãã€ãŠ300ã500nm
ã®æ³¢é·ç¯å²ã®èŒå°œçºå ã瀺ãèå äœã§ããããšã
æãŸãããæ¬çºæã«ãããŠå©çšãããèç©æ§èå
äœã·ãŒãã«çšããããèŒå°œæ§èå äœãšããŠã¯ãäº
䟡ã®ãŠãŒãããŠã ã«ãã賊掻ãããŠããã¢ã«ã«ãª
åé¡éå±åŒåããã²ã³åç©ç³»èå äœã§ããããšã
奜ãŸããããããã«éå®ããããã®ã§ã¯ãªãã
æ¬çºæã«ãããŠå©çšãããã®èç©æ§èå
äœã·ãŒ
ãã«çšããããèŒå°œæ§èå äœã®äŸãšããŠã¯ã ç±³åœç¹èš±ç¬¬3859527å·æ现æžã«èšèŒãããŠãã
SrSïŒCeïŒSmãSrSïŒEuïŒSmãThO2ïŒErãã
ãã³La2O2SïŒEuïŒSmãªã©ã®çµæåŒã§è¡šããã
ãèå äœã ç¹éæ55â12142å·å ¬å ±ã«èšèŒãããŠãã
ZnSïŒCuïŒPbãBaOã»xAl2O3ïŒEuããã ãã0.8
âŠïœâŠ10ããããã³ãM2+Oã»xSiO2ïŒïŒ¡ããã ãã
M2+ã¯MgãCaãSrãZnãCdããŸãã¯Baã§ããã
ã¯CeãTbãEuãTmãPbãTlãBiããŸãã¯
Mnã§ãããïœã¯ã0.5âŠïœâŠ2.5ã§ããããªã©ã®
çµæåŒã§è¡šããããèå äœã ç¹éæ55â12143å·å ¬å ±ã«èšèŒãããŠãã
ïŒBa1-x-yïŒMgxïŒCayïŒFXïŒaEu2+ããã ãã
ã¯Clããã³Brã®ãã¡ã®å°ãªããšãäžã€ã§ããã
ïœããã³ïœã¯ãïŒïŒïœïŒïœâŠ0.6ããã€xyâ ïŒã§
ãããïœã¯ã10-6âŠïœâŠïŒÃ10-2ã§ãããã®çµæ
åŒã§è¡šããããèå äœã ç¹éæ55â12144å·å ¬å ±ã«èšèŒãããŠãã
LnOXïŒxAããã ããLnã¯LaããGdãããã³
Luã®ãã¡ã®å°ãªããšãäžã€ãã¯Clããã³Brã®
ãã¡ã®å°ãªããšãäžã€ãã¯Ceããã³Tbã®ãã¡
ã®å°ãªããšãäžã€ããããŠãïœã¯ãïŒïŒïœïŒ0.1
ã§ãããã®çµæåŒã§è¡šããããèå äœã ç¹éæ55â12145å·å ¬å ±ã«èšèŒãããŠãã
ïŒBa1-xïŒMãïœïŒFXïŒyAããã ããMãã¯Mgã
CaãSrãZnãããã³Cdã®ãã¡ã®å°ãªããšãäž
ã€ãã¯ClãBrãããã³ïŒ©ã®ãã¡ã®å°ãªããšã
äžã€ãã¯EuãTbãCeãTmãDyãPrãHoã
NdãYbãããã³Erã®ãã¡ã®å°ãªããšãäžã€ãã
ããŠïœã¯ãïŒâŠïœâŠ0.6ãïœã¯ãïŒâŠïœâŠ0.2ã§ã
ããã®çµæåŒã§è¡šããããèå äœã ç¹éæ55â160078å·å ¬å ±ã«èšèŒãããŠããMã
FXã»xAïŒyLnããã ããMãã¯BaãCaãSrã
MgãZnãããã³Cdã®ãã¡ã®å°ãªããšãäžçš®ã
ã¯BeOãMgOãCaOãSrOãBaOãZnOã
Al2O3ãY2O3ãLa2O3ãIn2O3ãSiO2ãTiO2ã
ZrO2ãGeO2ãSnO2ãNb2O5ãTa2O5ãããã³
ThO2ã®ãã¡ã®å°ãªããšãäžçš®ãLnã¯EuãTbã
CeãTmãDyãPrãHoãNdãYbãErãSmã
ããã³Gdã®ãã¡ã®å°ãªããšãäžçš®ãã¯Clã
Brãããã³ïŒ©ã®ãã¡ã®å°ãªããšãäžçš®ã§ããã
ïœããã³ïœã¯ããããïŒÃ10-5âŠïœâŠ0.5ããã
ã³ïŒïŒïœâŠ0.2ã§ãããã®çµæåŒã§è¡šããããè
å äœã ç¹éæ56â116777å·å ¬å ±ã«èšèŒãããŠãã
ïŒBa1-xïŒMãïœïŒF2ã»aBaX2ïŒyEuïŒzAããã ãã
Mãã¯ããªãªãŠã ããã°ãã·ãŠã ãã«ã«ã·ãŠã ã
ã¹ããã³ããŠã ãäºéãããã³ã«ãããŠã ã®ãã¡
ã®å°ãªããšãäžçš®ãã¯å¡©çŽ ãèçŽ ãããã³æ²çŽ
ã®ãã¡ã®å°ãªããšãäžçš®ãã¯ãžã«ã³ããŠã ãã
ã³ã¹ã«ã³ãžãŠã ã®ãã¡ã®å°ãªããšãäžçš®ã§ããã
ïœãïœãïœãããã³ïœã¯ãããã0.5âŠïœâŠ1.25ã
ïŒâŠïœâŠïŒã10-6âŠïœâŠïŒÃ10-1ãããã³ïŒïŒïœ
âŠ10-2ã§ãããã®çµæåŒã§è¡šããããèå äœã ç¹éæ57â23673å·å ¬å ±ã«èšèŒãããŠãã
ïŒBa1-xïŒMãïœïŒF2ã»aBaX2ïŒyEuïŒzBããã ãã
Mãã¯ããªãªãŠã ããã°ãã·ãŠã ãã«ã«ã·ãŠã ã
ã¹ããã³ããŠã ãäºéãããã³ã«ãããŠã ã®ãã¡
ã®å°ãªããšãäžçš®ãã¯å¡©çŽ ãèçŽ ãããã³æ²çŽ
ã®ãã¡ã®å°ãªããšãäžçš®ã§ãããïœãïœãïœãã
ãã³ïœã¯ãããã0.5âŠïœâŠ1.25ãïŒâŠïœâŠïŒã
10-6âŠïœâŠïŒÃ10-1ãããã³ïŒïŒïœâŠïŒÃ10-1ã§
ãããã®çµæåŒã§è¡šããããèå äœã ç¹éæ57â23675å·å ¬å ±ã«èšèŒãããŠãã
ïŒBa1-xïŒMãïœïŒF2ã»aBaX2ïŒyEuïŒzAããã ãã
Mãã¯ããªãªãŠã ããã°ãã·ãŠã ãã«ã«ã·ãŠã ã
ã¹ããã³ããŠã ãäºéãããã³ã«ãããŠã ã®ãã¡
ã®å°ãªããšãäžçš®ãã¯å¡©çŽ ãèçŽ ãããã³æ²çŽ
ã®ãã¡ã®å°ãªããšãäžçš®ã§ãããã¯ç çŽ ããã³
ç¡ çŽ ã®ãã¡ã®å°ãªããšãäžçš®ã§ãããïœãïœã
ïœãããã³ïœã¯ãããã0.5âŠïœâŠ1.25ãïŒâŠïœ
âŠïŒã10-6âŠïœâŠïŒÃ10-1ãããã³ïŒïŒïœâŠïŒÃ
10-1ã§ãããã®çµæåŒã§è¡šããããèå äœã æ¬åºé¡äººã«ããç¹é¡æ56â167498å·æ现æžã«èš
èŒãããŠããMãOXïŒxCeããã ããMãã¯Prã
NdãPmãSmãEuãTbãDyãHoãErãTmã
Ybãããã³Biãããªã矀ããéžã°ããå°ãªããš
ãäžçš®ã®äžäŸ¡éå±ã§ãããã¯Clããã³Brã®ã
ã¡ã®ããããäžæ¹ãããã¯ãã®äž¡æ¹ã§ãããïœã¯
ïŒïŒïœïŒ0.1ã§ãããã®çµæåŒã§è¡šããããèå
äœã æ¬åºé¡äººã«ããç¹é¡æ57â89875å·æ现æžã«èš
èŒãããŠããBa1-xMx/2Lx/2FXïŒyEu2+ããã ãã
ïŒã¯ãLiãNaããRbãããã³Csãããªã矀ã
ãéžã°ããå°ãªããšãäžçš®ã®ã¢ã«ã«ãªéå±ãè¡šã
ãïŒïŒ¬ã¯ãScããLaãCeãPrãNdãPmã
SmãGdãTbãDyãHoãErãTmãYbãLuã
AlãGaãInãããã³Tlãããªã矀ããéžã°ãã
å°ãªããšãäžçš®ã®äžäŸ¡éå±ãè¡šããïŒïŒžã¯ãClã
Brãããã³ïŒ©ãããªã矀ããéžã°ããå°ãªããš
ãäžçš®ã®ããã²ã³ãè¡šããïŒãããŠãïœã¯10-2âŠ
ïœâŠ0.5ãïœã¯ïŒïŒïœâŠ0.1ã§ãããã®çµæåŒã§è¡š
ããããèå äœã æ¬åºé¡äººã«ããç¹é¡æ57â137374å·æ现æžã«èš
èŒãããŠããBaFXã»xAïŒyEu2+ããã ããã¯ã
ClãBrãããã³ïŒ©ãããªã矀ããéžã°ããå°ãª
ããšãäžçš®ã®ããã²ã³ã§ããïŒïŒ¡ã¯ãããã©ãã«
ãªãããŠé žååç©ã®çŒæç©ã§ããïŒãããŠãïœã¯
10-6âŠïœâŠ0.1ãïœã¯ïŒïŒïœâŠ0.1ã§ãããã®çµæ
åŒã§è¡šããããèå äœã æ¬åºé¡äººã«ããç¹é¡æ57â158048å·ïŒæå57幎
ïŒæ13æ¥åºé¡ïŒæ现æžã«èšèŒãããŠããBaFXã»
xAïŒyEu2+ããã ããã¯ãClãBrãããã³ïŒ©ã
ããªã矀ããéžã°ããå°ãªããšãäžçš®ã®ããã²ã³
ã§ããïŒïŒ¡ã¯ããããµãã«ãªãã±ã€é žããããµã
ã«ãªããã¿ã³é žããã³ãããµãã«ãªããžã«ã³ããŠ
ã é žã®äžäŸ¡ãããã¯äºäŸ¡éå±ã®å¡©ãããªããããµ
ãã«ãªãååç©çŸ€ããéžã°ããå°ãªããšãäžçš®ã®
ååç©ã®çŒæç©ã§ããïŒãããŠãïœã¯10-6âŠïœâŠ
0.1ãïœã¯ïŒïŒïœâŠ0.1ã§ãããã®çµæåŒã§è¡šãã
ããèå äœã ç¹éæ59â56479å·å ¬å ±ã«èšèŒãããŠãã
BaFXã»xNaXâ²ïŒaEu2+ããã ããããã³Xâ²ã¯ã
ããããClãBrãããã³ïŒ©ã®ãã¡ã®å°ãªããšã
äžçš®ã§ãããããã³ïœã¯ããããïŒïŒïœâŠïŒã
ããã³ïŒïŒïœâŠ0.2ã§ãããã®çµæåŒã§è¡šããã
ãèå äœã ç¹éæ59â56480å·å ¬å ±ã«èšèŒãããŠããMã
FXã»xNaXâ²ïŒyEu2+ïŒzAããã ããMãã¯Baã
Srãããã³Caãããªã矀ããéžã°ããå°ãªããš
ãäžçš®ã®ã¢ãªã«ãªåé¡éå±ã§ããïŒïŒžããã³
Xâ²ã¯ãããããClãBrãããã³ïŒ©ãããªã矀ã
ãéžã°ããå°ãªããšãäžçš®ã®ããã²ã³ã§ããïŒïŒ¡
ã¯ããCrãMnãFeãCoãããã³Niããéžã°
ããå°ãªããšãäžçš®ã®é·ç§»éå±ã§ããïŒãããŠã
ïœã¯ïŒïŒïœâŠïŒãïœã¯ïŒïŒïœâŠ0.2ãããã³ïœã¯
ïŒïŒïœâŠ10-2ã§ãããã®çµæåŒã§è¡šããããèå
äœã ç¹éæ59â75200å·å ¬å ±ã«èšèŒãããŠããMã
FXã»aMãXâ²ã»bMâ²ãXâ³2ã»cMã3ã»xAïŒ
yEu2+ããã ããMãã¯BaãSrãããã³Caãããª
ã矀ããéžã°ããå°ãªããšãäžçš®ã®ã¢ã«ã«ãªåé¡
éå±ã§ããïŒMãã¯LiãNaããRbãããã³Cs
ãããªã矀ããéžã°ããå°ãªããšãäžçš®ã®ã¢ã«ã«
ãªéå±ã§ããïŒMâ²ãã¯Beããã³Mgãããªã矀ã
ãéžã°ããå°ãªããšãäžçš®ã®äºäŸ¡éå±ã§ããïŒ
Mãã¯AlãGaãInãããã³Tlãããªã矀ããéž
ã°ããå°ãªããšãäžçš®ã®äžäŸ¡éå±ã§ããïŒïŒ¡ã¯é
å±é žåç©ã§ããïŒïŒžã¯ClãBrãããã³ïŒ©ãããª
ã矀ããéžã°ããå°ãªããšãäžçš®ã®ããã²ã³ã§ã
ãïŒXâ²ãXâ³ãããã³ïŒžã¯ããClãBrããã
ã³ïŒ©ãããªã矀ããéžã°ããå°ãªããšãäžçš®ã®ã
ãã²ã³ã§ããïŒãããŠãïœã¯ïŒâŠïœâŠïŒãïœã¯ïŒ
âŠïœâŠ10-2ãïœã¯ïŒâŠïœâŠ10-2ããã€ïœïŒïœïŒïœ
â§10-6ã§ããïŒïœã¯ïŒïŒïœâŠ0.5ãïœã¯ïŒïŒïœâŠ
0.2ã§ãããã®çµæåŒã§è¡šããããèå äœã ãªã©ãæããããšãã§ããã
ãã«çšããããèŒå°œæ§èå äœã®äŸãšããŠã¯ã ç±³åœç¹èš±ç¬¬3859527å·æ现æžã«èšèŒãããŠãã
SrSïŒCeïŒSmãSrSïŒEuïŒSmãThO2ïŒErãã
ãã³La2O2SïŒEuïŒSmãªã©ã®çµæåŒã§è¡šããã
ãèå äœã ç¹éæ55â12142å·å ¬å ±ã«èšèŒãããŠãã
ZnSïŒCuïŒPbãBaOã»xAl2O3ïŒEuããã ãã0.8
âŠïœâŠ10ããããã³ãM2+Oã»xSiO2ïŒïŒ¡ããã ãã
M2+ã¯MgãCaãSrãZnãCdããŸãã¯Baã§ããã
ã¯CeãTbãEuãTmãPbãTlãBiããŸãã¯
Mnã§ãããïœã¯ã0.5âŠïœâŠ2.5ã§ããããªã©ã®
çµæåŒã§è¡šããããèå äœã ç¹éæ55â12143å·å ¬å ±ã«èšèŒãããŠãã
ïŒBa1-x-yïŒMgxïŒCayïŒFXïŒaEu2+ããã ãã
ã¯Clããã³Brã®ãã¡ã®å°ãªããšãäžã€ã§ããã
ïœããã³ïœã¯ãïŒïŒïœïŒïœâŠ0.6ããã€xyâ ïŒã§
ãããïœã¯ã10-6âŠïœâŠïŒÃ10-2ã§ãããã®çµæ
åŒã§è¡šããããèå äœã ç¹éæ55â12144å·å ¬å ±ã«èšèŒãããŠãã
LnOXïŒxAããã ããLnã¯LaããGdãããã³
Luã®ãã¡ã®å°ãªããšãäžã€ãã¯Clããã³Brã®
ãã¡ã®å°ãªããšãäžã€ãã¯Ceããã³Tbã®ãã¡
ã®å°ãªããšãäžã€ããããŠãïœã¯ãïŒïŒïœïŒ0.1
ã§ãããã®çµæåŒã§è¡šããããèå äœã ç¹éæ55â12145å·å ¬å ±ã«èšèŒãããŠãã
ïŒBa1-xïŒMãïœïŒFXïŒyAããã ããMãã¯Mgã
CaãSrãZnãããã³Cdã®ãã¡ã®å°ãªããšãäž
ã€ãã¯ClãBrãããã³ïŒ©ã®ãã¡ã®å°ãªããšã
äžã€ãã¯EuãTbãCeãTmãDyãPrãHoã
NdãYbãããã³Erã®ãã¡ã®å°ãªããšãäžã€ãã
ããŠïœã¯ãïŒâŠïœâŠ0.6ãïœã¯ãïŒâŠïœâŠ0.2ã§ã
ããã®çµæåŒã§è¡šããããèå äœã ç¹éæ55â160078å·å ¬å ±ã«èšèŒãããŠããMã
FXã»xAïŒyLnããã ããMãã¯BaãCaãSrã
MgãZnãããã³Cdã®ãã¡ã®å°ãªããšãäžçš®ã
ã¯BeOãMgOãCaOãSrOãBaOãZnOã
Al2O3ãY2O3ãLa2O3ãIn2O3ãSiO2ãTiO2ã
ZrO2ãGeO2ãSnO2ãNb2O5ãTa2O5ãããã³
ThO2ã®ãã¡ã®å°ãªããšãäžçš®ãLnã¯EuãTbã
CeãTmãDyãPrãHoãNdãYbãErãSmã
ããã³Gdã®ãã¡ã®å°ãªããšãäžçš®ãã¯Clã
Brãããã³ïŒ©ã®ãã¡ã®å°ãªããšãäžçš®ã§ããã
ïœããã³ïœã¯ããããïŒÃ10-5âŠïœâŠ0.5ããã
ã³ïŒïŒïœâŠ0.2ã§ãããã®çµæåŒã§è¡šããããè
å äœã ç¹éæ56â116777å·å ¬å ±ã«èšèŒãããŠãã
ïŒBa1-xïŒMãïœïŒF2ã»aBaX2ïŒyEuïŒzAããã ãã
Mãã¯ããªãªãŠã ããã°ãã·ãŠã ãã«ã«ã·ãŠã ã
ã¹ããã³ããŠã ãäºéãããã³ã«ãããŠã ã®ãã¡
ã®å°ãªããšãäžçš®ãã¯å¡©çŽ ãèçŽ ãããã³æ²çŽ
ã®ãã¡ã®å°ãªããšãäžçš®ãã¯ãžã«ã³ããŠã ãã
ã³ã¹ã«ã³ãžãŠã ã®ãã¡ã®å°ãªããšãäžçš®ã§ããã
ïœãïœãïœãããã³ïœã¯ãããã0.5âŠïœâŠ1.25ã
ïŒâŠïœâŠïŒã10-6âŠïœâŠïŒÃ10-1ãããã³ïŒïŒïœ
âŠ10-2ã§ãããã®çµæåŒã§è¡šããããèå äœã ç¹éæ57â23673å·å ¬å ±ã«èšèŒãããŠãã
ïŒBa1-xïŒMãïœïŒF2ã»aBaX2ïŒyEuïŒzBããã ãã
Mãã¯ããªãªãŠã ããã°ãã·ãŠã ãã«ã«ã·ãŠã ã
ã¹ããã³ããŠã ãäºéãããã³ã«ãããŠã ã®ãã¡
ã®å°ãªããšãäžçš®ãã¯å¡©çŽ ãèçŽ ãããã³æ²çŽ
ã®ãã¡ã®å°ãªããšãäžçš®ã§ãããïœãïœãïœãã
ãã³ïœã¯ãããã0.5âŠïœâŠ1.25ãïŒâŠïœâŠïŒã
10-6âŠïœâŠïŒÃ10-1ãããã³ïŒïŒïœâŠïŒÃ10-1ã§
ãããã®çµæåŒã§è¡šããããèå äœã ç¹éæ57â23675å·å ¬å ±ã«èšèŒãããŠãã
ïŒBa1-xïŒMãïœïŒF2ã»aBaX2ïŒyEuïŒzAããã ãã
Mãã¯ããªãªãŠã ããã°ãã·ãŠã ãã«ã«ã·ãŠã ã
ã¹ããã³ããŠã ãäºéãããã³ã«ãããŠã ã®ãã¡
ã®å°ãªããšãäžçš®ãã¯å¡©çŽ ãèçŽ ãããã³æ²çŽ
ã®ãã¡ã®å°ãªããšãäžçš®ã§ãããã¯ç çŽ ããã³
ç¡ çŽ ã®ãã¡ã®å°ãªããšãäžçš®ã§ãããïœãïœã
ïœãããã³ïœã¯ãããã0.5âŠïœâŠ1.25ãïŒâŠïœ
âŠïŒã10-6âŠïœâŠïŒÃ10-1ãããã³ïŒïŒïœâŠïŒÃ
10-1ã§ãããã®çµæåŒã§è¡šããããèå äœã æ¬åºé¡äººã«ããç¹é¡æ56â167498å·æ现æžã«èš
èŒãããŠããMãOXïŒxCeããã ããMãã¯Prã
NdãPmãSmãEuãTbãDyãHoãErãTmã
Ybãããã³Biãããªã矀ããéžã°ããå°ãªããš
ãäžçš®ã®äžäŸ¡éå±ã§ãããã¯Clããã³Brã®ã
ã¡ã®ããããäžæ¹ãããã¯ãã®äž¡æ¹ã§ãããïœã¯
ïŒïŒïœïŒ0.1ã§ãããã®çµæåŒã§è¡šããããèå
äœã æ¬åºé¡äººã«ããç¹é¡æ57â89875å·æ现æžã«èš
èŒãããŠããBa1-xMx/2Lx/2FXïŒyEu2+ããã ãã
ïŒã¯ãLiãNaããRbãããã³Csãããªã矀ã
ãéžã°ããå°ãªããšãäžçš®ã®ã¢ã«ã«ãªéå±ãè¡šã
ãïŒïŒ¬ã¯ãScããLaãCeãPrãNdãPmã
SmãGdãTbãDyãHoãErãTmãYbãLuã
AlãGaãInãããã³Tlãããªã矀ããéžã°ãã
å°ãªããšãäžçš®ã®äžäŸ¡éå±ãè¡šããïŒïŒžã¯ãClã
Brãããã³ïŒ©ãããªã矀ããéžã°ããå°ãªããš
ãäžçš®ã®ããã²ã³ãè¡šããïŒãããŠãïœã¯10-2âŠ
ïœâŠ0.5ãïœã¯ïŒïŒïœâŠ0.1ã§ãããã®çµæåŒã§è¡š
ããããèå äœã æ¬åºé¡äººã«ããç¹é¡æ57â137374å·æ现æžã«èš
èŒãããŠããBaFXã»xAïŒyEu2+ããã ããã¯ã
ClãBrãããã³ïŒ©ãããªã矀ããéžã°ããå°ãª
ããšãäžçš®ã®ããã²ã³ã§ããïŒïŒ¡ã¯ãããã©ãã«
ãªãããŠé žååç©ã®çŒæç©ã§ããïŒãããŠãïœã¯
10-6âŠïœâŠ0.1ãïœã¯ïŒïŒïœâŠ0.1ã§ãããã®çµæ
åŒã§è¡šããããèå äœã æ¬åºé¡äººã«ããç¹é¡æ57â158048å·ïŒæå57幎
ïŒæ13æ¥åºé¡ïŒæ现æžã«èšèŒãããŠããBaFXã»
xAïŒyEu2+ããã ããã¯ãClãBrãããã³ïŒ©ã
ããªã矀ããéžã°ããå°ãªããšãäžçš®ã®ããã²ã³
ã§ããïŒïŒ¡ã¯ããããµãã«ãªãã±ã€é žããããµã
ã«ãªããã¿ã³é žããã³ãããµãã«ãªããžã«ã³ããŠ
ã é žã®äžäŸ¡ãããã¯äºäŸ¡éå±ã®å¡©ãããªããããµ
ãã«ãªãååç©çŸ€ããéžã°ããå°ãªããšãäžçš®ã®
ååç©ã®çŒæç©ã§ããïŒãããŠãïœã¯10-6âŠïœâŠ
0.1ãïœã¯ïŒïŒïœâŠ0.1ã§ãããã®çµæåŒã§è¡šãã
ããèå äœã ç¹éæ59â56479å·å ¬å ±ã«èšèŒãããŠãã
BaFXã»xNaXâ²ïŒaEu2+ããã ããããã³Xâ²ã¯ã
ããããClãBrãããã³ïŒ©ã®ãã¡ã®å°ãªããšã
äžçš®ã§ãããããã³ïœã¯ããããïŒïŒïœâŠïŒã
ããã³ïŒïŒïœâŠ0.2ã§ãããã®çµæåŒã§è¡šããã
ãèå äœã ç¹éæ59â56480å·å ¬å ±ã«èšèŒãããŠããMã
FXã»xNaXâ²ïŒyEu2+ïŒzAããã ããMãã¯Baã
Srãããã³Caãããªã矀ããéžã°ããå°ãªããš
ãäžçš®ã®ã¢ãªã«ãªåé¡éå±ã§ããïŒïŒžããã³
Xâ²ã¯ãããããClãBrãããã³ïŒ©ãããªã矀ã
ãéžã°ããå°ãªããšãäžçš®ã®ããã²ã³ã§ããïŒïŒ¡
ã¯ããCrãMnãFeãCoãããã³Niããéžã°
ããå°ãªããšãäžçš®ã®é·ç§»éå±ã§ããïŒãããŠã
ïœã¯ïŒïŒïœâŠïŒãïœã¯ïŒïŒïœâŠ0.2ãããã³ïœã¯
ïŒïŒïœâŠ10-2ã§ãããã®çµæåŒã§è¡šããããèå
äœã ç¹éæ59â75200å·å ¬å ±ã«èšèŒãããŠããMã
FXã»aMãXâ²ã»bMâ²ãXâ³2ã»cMã3ã»xAïŒ
yEu2+ããã ããMãã¯BaãSrãããã³Caãããª
ã矀ããéžã°ããå°ãªããšãäžçš®ã®ã¢ã«ã«ãªåé¡
éå±ã§ããïŒMãã¯LiãNaããRbãããã³Cs
ãããªã矀ããéžã°ããå°ãªããšãäžçš®ã®ã¢ã«ã«
ãªéå±ã§ããïŒMâ²ãã¯Beããã³Mgãããªã矀ã
ãéžã°ããå°ãªããšãäžçš®ã®äºäŸ¡éå±ã§ããïŒ
Mãã¯AlãGaãInãããã³Tlãããªã矀ããéž
ã°ããå°ãªããšãäžçš®ã®äžäŸ¡éå±ã§ããïŒïŒ¡ã¯é
å±é žåç©ã§ããïŒïŒžã¯ClãBrãããã³ïŒ©ãããª
ã矀ããéžã°ããå°ãªããšãäžçš®ã®ããã²ã³ã§ã
ãïŒXâ²ãXâ³ãããã³ïŒžã¯ããClãBrããã
ã³ïŒ©ãããªã矀ããéžã°ããå°ãªããšãäžçš®ã®ã
ãã²ã³ã§ããïŒãããŠãïœã¯ïŒâŠïœâŠïŒãïœã¯ïŒ
âŠïœâŠ10-2ãïœã¯ïŒâŠïœâŠ10-2ããã€ïœïŒïœïŒïœ
â§10-6ã§ããïŒïœã¯ïŒïŒïœâŠ0.5ãïœã¯ïŒïŒïœâŠ
0.2ã§ãããã®çµæåŒã§è¡šããããèå äœã ãªã©ãæããããšãã§ããã
ãã ããæ¬çºæã«çšããããèŒå°œæ§èå
äœã¯äž
è¿°ã®èå äœã«éããããã®ã§ã¯ãªããæŸå°ç·ãç §
å°ããã®ã¡ã«å±èµ·å ãç §å°ããå Žåã«ãèŒå°œçºå
ã瀺ãèå äœã§ããã°ãããªããã®ã§ãã€ãŠãã
ãã
è¿°ã®èå äœã«éããããã®ã§ã¯ãªããæŸå°ç·ãç §
å°ããã®ã¡ã«å±èµ·å ãç §å°ããå Žåã«ãèŒå°œçºå
ã瀺ãèå äœã§ããã°ãããªããã®ã§ãã€ãŠãã
ãã
ãŸãèå
äœå±€ã®çµåå€ã®äŸãšããŠã¯ããŒã©ãã³
çã®èçœè³ªãããã¹ãã©ã³çã®ããªãµãã«ã©ã€
ãããŸãã¯ã¢ã©ãã¢ãŽã ã®ãããªå€©ç¶é«ååç©
質ïŒããã³ãããªããã«ããã©ãŒã«ãããªé ¢é žã
ãã«ããããã»ã«ããŒã¹ããšãã«ã»ã«ããŒã¹ãå¡©
åãããªãã³ã»å¡©åããã«ã³ããªããŒãããªã¡ã
ã«ã¡ã¿ã¯ãªã¬ãŒããå¡©åããã«ã»é ¢é žããã«ã³ã
ãªããŒãããªãŠã¬ã¿ã³ãã»ã«ããŒã¹ã¢ã»ããŒãã
ãã¬ãŒããããªããã«ã¢ã«ã³ãŒã«ãç·ç¶ããªãšã¹
ãã«ãªã©ãããªåæé«ååç©è³ªãªã©ã«ãã代衚ã
ããçµåå€ãæããããšãã§ããããã®ãããªçµ
åå€ã®ãªãã§ç¹ã«å¥œãŸãããã®ã¯ããããã»ã«ã
ãŒã¹ãç·ç¶ããªãšã¹ãã«ãããã³ãããã»ã«ããŒ
ã¹ãšç·ç¶ããªãšã¹ãã«ãšã®æ··åç©ã§ããã
çã®èçœè³ªãããã¹ãã©ã³çã®ããªãµãã«ã©ã€
ãããŸãã¯ã¢ã©ãã¢ãŽã ã®ãããªå€©ç¶é«ååç©
質ïŒããã³ãããªããã«ããã©ãŒã«ãããªé ¢é žã
ãã«ããããã»ã«ããŒã¹ããšãã«ã»ã«ããŒã¹ãå¡©
åãããªãã³ã»å¡©åããã«ã³ããªããŒãããªã¡ã
ã«ã¡ã¿ã¯ãªã¬ãŒããå¡©åããã«ã»é ¢é žããã«ã³ã
ãªããŒãããªãŠã¬ã¿ã³ãã»ã«ããŒã¹ã¢ã»ããŒãã
ãã¬ãŒããããªããã«ã¢ã«ã³ãŒã«ãç·ç¶ããªãšã¹
ãã«ãªã©ãããªåæé«ååç©è³ªãªã©ã«ãã代衚ã
ããçµåå€ãæããããšãã§ããããã®ãããªçµ
åå€ã®ãªãã§ç¹ã«å¥œãŸãããã®ã¯ããããã»ã«ã
ãŒã¹ãç·ç¶ããªãšã¹ãã«ãããã³ãããã»ã«ããŒ
ã¹ãšç·ç¶ããªãšã¹ãã«ãšã®æ··åç©ã§ããã
èå
äœå±€ã¯ãããšãã°ã次ã®ãããªæ¹æ³ã«ãã
æ¯æäœäžã«åœ¢æããããšãã§ããã
æ¯æäœäžã«åœ¢æããããšãã§ããã
ãŸãäžèšã®èŒå°œæ§èå
äœç²åãšçµåå€ãšãé©åœ
ãªæº¶å€ïŒããšãã°ãäœçŽã¢ã«ã³ãŒã«ãå¡©çŽ ååå«
æçåæ°ŽçŽ ãã±ãã³ããšã¹ãã«ããšãŒãã«ïŒã«å
ãããããå åã«æ··åããŠãçµåå€æº¶æ¶²äžã«èå
äœç²åãåäžã«åæ£ããå¡åžæ¶²ã調補ããã
ãªæº¶å€ïŒããšãã°ãäœçŽã¢ã«ã³ãŒã«ãå¡©çŽ ååå«
æçåæ°ŽçŽ ãã±ãã³ããšã¹ãã«ããšãŒãã«ïŒã«å
ãããããå åã«æ··åããŠãçµåå€æº¶æ¶²äžã«èå
äœç²åãåäžã«åæ£ããå¡åžæ¶²ã調補ããã
å¡åžæ¶²ã«ãããçµåå€ãšèŒå°œæ§èå
äœç²åãšã®
æ··åæ¯ã¯ãç®çãšããèç©æ§èå äœã·ãŒãã®ç¹
æ§ãèå äœç²åã®çš®é¡ãªã©ã«ãã€ãŠç°ãªãããäž
è¬ã«ã¯çµåå€ãšèå äœç²åãšã®æ··åæ¯ã¯ãïŒïŒïŒ
ä¹è³ïŒïŒ100ïŒééæ¯ïŒã®ç¯å²ããéžã°ãããããŠ
ç¹ã«ïŒïŒïŒä¹è³ïŒïŒ40ïŒééæ¯ïŒã®ç¯å²ããéžã¶
ããšã奜ãŸããã
æ··åæ¯ã¯ãç®çãšããèç©æ§èå äœã·ãŒãã®ç¹
æ§ãèå äœç²åã®çš®é¡ãªã©ã«ãã€ãŠç°ãªãããäž
è¬ã«ã¯çµåå€ãšèå äœç²åãšã®æ··åæ¯ã¯ãïŒïŒïŒ
ä¹è³ïŒïŒ100ïŒééæ¯ïŒã®ç¯å²ããéžã°ãããããŠ
ç¹ã«ïŒïŒïŒä¹è³ïŒïŒ40ïŒééæ¯ïŒã®ç¯å²ããéžã¶
ããšã奜ãŸããã
ãªããå¡åžæ¶²ã«ã¯ã該å¡åžæ¶²äžã«ãããèå
äœ
ç²åã®åæ£æ§ãåäžãããããã®åæ£å€ããŸãã
圢æåŸã®èå äœå±€äžã«ãããçµåå€ãšèå äœç²å
ãšã®éã®çµååãåäžãããããã®å¯å¡å€ãªã©ã®
çš®ã ã®æ·»å å€ãæ··åãããŠããŠãããããã®ãã
ãªç®çã«çšããããåæ£å€ã®äŸãšããŠã¯ããã¿ã«
é žãã¹ãã¢ãªã³é žãã«ããã³é žã芪油æ§çé¢æŽ»æ§
å€ãªã©ãæããããšãã§ããããããŠå¯å¡å€ã®äŸ
ãšããŠã¯ãçé žããªããšãã«ãçé žããªã¯ã¬ãž
ã«ãçé žãžããšãã«ãªã©ã®çé žãšã¹ãã«ïŒãã¿ã«
é žãžãšãã«ããã¿ã«é žãžã¡ããã·ãšãã«ãªã©ã®ã
ã¿ã«é žãšã¹ãã«ïŒã°ãªã³ãŒã«é žãšãã«ãã¿ãªã«ãš
ãã«ãã°ãªã³ãŒã«é žããã«ãã¿ãªã«ããã«ãªã©ã®
ã°ãªã³ãŒã«é žãšã¹ãã«ïŒãããŠãããªãšãã¬ã³ã°
ãªã³ãŒã«ãšã¢ãžãã³é žãšã®ããªãšã¹ãã«ããžãšã
ã¬ã³ã°ãªã³ãŒã«ãšã³ãã¯é žãšã®ããªãšã¹ãã«ãªã©
ã®ããªãšãã¬ã³ã°ãªã³ãŒã«ãšèèªæäºå¡©åºé žãšã®
ããªãšã¹ãã«ãªã©ãæããããšãã§ããã
ç²åã®åæ£æ§ãåäžãããããã®åæ£å€ããŸãã
圢æåŸã®èå äœå±€äžã«ãããçµåå€ãšèå äœç²å
ãšã®éã®çµååãåäžãããããã®å¯å¡å€ãªã©ã®
çš®ã ã®æ·»å å€ãæ··åãããŠããŠãããããã®ãã
ãªç®çã«çšããããåæ£å€ã®äŸãšããŠã¯ããã¿ã«
é žãã¹ãã¢ãªã³é žãã«ããã³é žã芪油æ§çé¢æŽ»æ§
å€ãªã©ãæããããšãã§ããããããŠå¯å¡å€ã®äŸ
ãšããŠã¯ãçé žããªããšãã«ãçé žããªã¯ã¬ãž
ã«ãçé žãžããšãã«ãªã©ã®çé žãšã¹ãã«ïŒãã¿ã«
é žãžãšãã«ããã¿ã«é žãžã¡ããã·ãšãã«ãªã©ã®ã
ã¿ã«é žãšã¹ãã«ïŒã°ãªã³ãŒã«é žãšãã«ãã¿ãªã«ãš
ãã«ãã°ãªã³ãŒã«é žããã«ãã¿ãªã«ããã«ãªã©ã®
ã°ãªã³ãŒã«é žãšã¹ãã«ïŒãããŠãããªãšãã¬ã³ã°
ãªã³ãŒã«ãšã¢ãžãã³é žãšã®ããªãšã¹ãã«ããžãšã
ã¬ã³ã°ãªã³ãŒã«ãšã³ãã¯é žãšã®ããªãšã¹ãã«ãªã©
ã®ããªãšãã¬ã³ã°ãªã³ãŒã«ãšèèªæäºå¡©åºé žãšã®
ããªãšã¹ãã«ãªã©ãæããããšãã§ããã
äžèšã®ããã«ããŠèª¿è£œãããèå
äœç²åãšçµå
å€ãšãå«æããå¡åžæ¶²ãã次ã«ãæ¯æäœã®è¡šé¢ã«
åäžã«å¡åžããããšã«ããå¡åžæ¶²ã®å¡èã圢æã
ãããã®å¡åžæäœã¯ãéåžžã®å¡åžæ段ãããšã
ã°ããã¯ã¿ãŒãã¬ãŒããããŒã«ã³ãŒã¿ãŒããã€ã
ã³ãŒã¿ãŒãªã©ãçšããããšã«ããè¡ãªãããšãã§
ããã
å€ãšãå«æããå¡åžæ¶²ãã次ã«ãæ¯æäœã®è¡šé¢ã«
åäžã«å¡åžããããšã«ããå¡åžæ¶²ã®å¡èã圢æã
ãããã®å¡åžæäœã¯ãéåžžã®å¡åžæ段ãããšã
ã°ããã¯ã¿ãŒãã¬ãŒããããŒã«ã³ãŒã¿ãŒããã€ã
ã³ãŒã¿ãŒãªã©ãçšããããšã«ããè¡ãªãããšãã§
ããã
ã€ãã§ã圢æãããå¡èãåŸã
ã«å ç±ããããš
ã«ãã也ç¥ããŠãæ¯æäœäžãžã®èå äœå±€ã®åœ¢æã
å®äºãããèå äœå±€ã®å±€åã¯ãç®çãšããèç©æ§
èå äœã·ãŒãã®ç¹æ§ãèå äœç²åã®çš®é¡ãçµåå€
ãšèå äœç²åãšã®æ··åæ¯ãªã©ã«ãã€ãŠç°ãªããã
éåžžã¯20ÎŒmä¹è³ïŒmmãšããããã ãããã®å±€å
ã¯50ä¹è³500ÎŒmãšããã®ã奜ãŸããã
ã«ãã也ç¥ããŠãæ¯æäœäžãžã®èå äœå±€ã®åœ¢æã
å®äºãããèå äœå±€ã®å±€åã¯ãç®çãšããèç©æ§
èå äœã·ãŒãã®ç¹æ§ãèå äœç²åã®çš®é¡ãçµåå€
ãšèå äœç²åãšã®æ··åæ¯ãªã©ã«ãã€ãŠç°ãªããã
éåžžã¯20ÎŒmä¹è³ïŒmmãšããããã ãããã®å±€å
ã¯50ä¹è³500ÎŒmãšããã®ã奜ãŸããã
ãªããèå
äœå±€ã¯ãå¿
ãããäžèšã®ããã«æ¯æ
äœäžã«å¡åžæ¶²ãçŽæ¥å¡åžããŠåœ¢æããå¿ èŠã¯ãª
ããããšãã°ãå¥ã«ãã¬ã©ã¹æ¿ãéå±æ¿ããã©ã¹
ããã¯ã·ãŒããªã©ã®ã·ãŒãäžã«å¡åžæ¶²ãå¡åžã也
ç¥ããããšã«ããèå äœå±€ã圢æããåŸããã
ããæ¯æäœäžã«æŒå§ãããããããã¯æ¥çå€ãçš
ããæ¹æ³ãªã©ã«ããæ¯æäœãšèå äœå±€ãšãæ¥åã
ãŠãããã
äœäžã«å¡åžæ¶²ãçŽæ¥å¡åžããŠåœ¢æããå¿ èŠã¯ãª
ããããšãã°ãå¥ã«ãã¬ã©ã¹æ¿ãéå±æ¿ããã©ã¹
ããã¯ã·ãŒããªã©ã®ã·ãŒãäžã«å¡åžæ¶²ãå¡åžã也
ç¥ããããšã«ããèå äœå±€ã圢æããåŸããã
ããæ¯æäœäžã«æŒå§ãããããããã¯æ¥çå€ãçš
ããæ¹æ³ãªã©ã«ããæ¯æäœãšèå äœå±€ãšãæ¥åã
ãŠãããã
èå
äœå±€ã®äžã«ã¯åèšã®ããã«ä¿è·èãèšãã
ããŠããããšã奜ãŸããããã®ä¿è·èã¯ãããšã
ã°ãé ¢é žã»ã«ããŒã¹ããããã»ã«ããŒã¹ãªã©ã®é
æãªã»ã«ããŒã¹èªå°äœïŒããªã¡ãã«ã¡ã¿ã¯ãªã¬ãŒ
ããããªããã«ããã©ãŒã«ãããªããã«ãã«ããŒ
ã«ãããªã«ãŒãããŒããããªé ¢é žããã«ã»é ¢é žã
ãã«ã³ããªããŒãããªãšãã¬ãã¬ãã¿ã¬ãŒããã
ãªãšãã¬ã³ãããªå¡©åãããªãã³ãããªã¢ãããª
ã©ã®éæãªåæé«ååç©è³ªãã圢æããããã®ã§
ãããä¿è·èã®èåã¯ãéåžž0.1ä¹è³100ÎŒmã奜
ãŸããã¯0.3ä¹è³50ÎŒmãšãããã
ããŠããããšã奜ãŸããããã®ä¿è·èã¯ãããšã
ã°ãé ¢é žã»ã«ããŒã¹ããããã»ã«ããŒã¹ãªã©ã®é
æãªã»ã«ããŒã¹èªå°äœïŒããªã¡ãã«ã¡ã¿ã¯ãªã¬ãŒ
ããããªããã«ããã©ãŒã«ãããªããã«ãã«ããŒ
ã«ãããªã«ãŒãããŒããããªé ¢é žããã«ã»é ¢é žã
ãã«ã³ããªããŒãããªãšãã¬ãã¬ãã¿ã¬ãŒããã
ãªãšãã¬ã³ãããªå¡©åãããªãã³ãããªã¢ãããª
ã©ã®éæãªåæé«ååç©è³ªãã圢æããããã®ã§
ãããä¿è·èã®èåã¯ãéåžž0.1ä¹è³100ÎŒmã奜
ãŸããã¯0.3ä¹è³50ÎŒmãšãããã
æ¬çºæã®ãªãŒãã©ãžãªã°ã©ãã€ãŒçšæž¬å®ããã
ã®ããäžæ¹ã®æ§æéšæã§ããæ¯æäœãããªãã¡ã
æŸå°æ§æšèãä»ãããçç©äœç±æ¥ã®ç©è³ªãåé¢å±
éããããã®æ¯æäœã¯ãåŸæ¥ã®ãªãŒãã©ãžãªã°ã©
ãã€ãŒæè¡ã«ãããŠå©çšãããŠãããããããã¯
ãã®å©çšãææ¡ãããŠããåçš®ã®åé¢å±éçšæ¯æ
äœããä»»æã«éžæããããšãã§ããããã®ãããª
åé¢å±éçšæ¯æäœã®äŸãšããŠã¯ãã²ã«ç¶æ¯æäœ
ïŒåœ¢ç¶ã¯å±€ç¶ãæ±ç¶ãªã©ä»»æïŒãã¢ã»ããŒãèãªã©
ã®ããªããŒæ圢äœããããã¯æ¿ŸçŽãªã©ã®åçš®ã®æ¯
æäœã®åœ¢æ ã®é»æ°æ³³ååé¢çšæ¯æäœããããŠã·ãª
ã«ã²ã«ãªã©ãããªãèå±€ã¯ãããã°ã©ãã€ãŒçšæ¯
æäœãæããããšãã§ããããããã®å±éåé¢çš
æ¯æäœã¯ãéåžžã¯ä¹Ÿç¥ç©ãšããŠæ¬çºæã®æž¬å®çšã
ãããæ§æããããææã«ãããããšãã°ãåé¢
å±éçšã®æº¶åªãªã©ãå«æµžãããç¶æ ã§ããããæ§
æããŠããŠãè¯ãããŸãããããã®åé¢å±éçšæ¯
æäœã«ã¯ãã¬ã©ã¹æ¿ããã©ã¹ããã¯ã·ãŒããªã©ã
ããªãæ¯æè£å©å ·ãä»èšãããŠããŠãããã
ã®ããäžæ¹ã®æ§æéšæã§ããæ¯æäœãããªãã¡ã
æŸå°æ§æšèãä»ãããçç©äœç±æ¥ã®ç©è³ªãåé¢å±
éããããã®æ¯æäœã¯ãåŸæ¥ã®ãªãŒãã©ãžãªã°ã©
ãã€ãŒæè¡ã«ãããŠå©çšãããŠãããããããã¯
ãã®å©çšãææ¡ãããŠããåçš®ã®åé¢å±éçšæ¯æ
äœããä»»æã«éžæããããšãã§ããããã®ãããª
åé¢å±éçšæ¯æäœã®äŸãšããŠã¯ãã²ã«ç¶æ¯æäœ
ïŒåœ¢ç¶ã¯å±€ç¶ãæ±ç¶ãªã©ä»»æïŒãã¢ã»ããŒãèãªã©
ã®ããªããŒæ圢äœããããã¯æ¿ŸçŽãªã©ã®åçš®ã®æ¯
æäœã®åœ¢æ ã®é»æ°æ³³ååé¢çšæ¯æäœããããŠã·ãª
ã«ã²ã«ãªã©ãããªãèå±€ã¯ãããã°ã©ãã€ãŒçšæ¯
æäœãæããããšãã§ããããããã®å±éåé¢çš
æ¯æäœã¯ãéåžžã¯ä¹Ÿç¥ç©ãšããŠæ¬çºæã®æž¬å®çšã
ãããæ§æããããææã«ãããããšãã°ãåé¢
å±éçšã®æº¶åªãªã©ãå«æµžãããç¶æ ã§ããããæ§
æããŠããŠãè¯ãããŸãããããã®åé¢å±éçšæ¯
æäœã«ã¯ãã¬ã©ã¹æ¿ããã©ã¹ããã¯ã·ãŒããªã©ã
ããªãæ¯æè£å©å ·ãä»èšãããŠããŠãããã
ãªããåé¢å±éçšæ¯æäœã¯äžèšã«äŸç€ºããæ¯æ
äœã«éå®ããããã®ã§ã¯ãªãããªãŒãã©ãžãªã°ã©
ãã€ãŒæè¡ã«ãããŠè©Šæã®åé¢å±éã«å©çšã§ãã
ãã®ã§ããã°ä»»æã«çšããããšãã§ããã
äœã«éå®ããããã®ã§ã¯ãªãããªãŒãã©ãžãªã°ã©
ãã€ãŒæè¡ã«ãããŠè©Šæã®åé¢å±éã«å©çšã§ãã
ãã®ã§ããã°ä»»æã«çšããããšãã§ããã
次ã«ãæ¬çºæã®ãªãŒãã©ãžãªã°ã©ãã€ãŒæž¬å®çš
ããããçšãããªãŒãã©ãžãªã°ã©ãã€ãŒæž¬å®æäœ
ã«ã€ããŠèª¬æããã
ããããçšãããªãŒãã©ãžãªã°ã©ãã€ãŒæž¬å®æäœ
ã«ã€ããŠèª¬æããã
æ¬çºæã®ãªãŒãã©ãžãªã°ã©ãã€ãŒã«çšãããã
æŸå°æ§æšèç©è³ªã¯ã枬å®å¯Ÿè±¡ã®è©Šæã«é©åœãªæ¹æ³
ã§æŸå°æ§å çŽ ãä¿æãããããšã«ãã€ãŠåŸãã
ãã
æŸå°æ§æšèç©è³ªã¯ã枬å®å¯Ÿè±¡ã®è©Šæã«é©åœãªæ¹æ³
ã§æŸå°æ§å çŽ ãä¿æãããããšã«ãã€ãŠåŸãã
ãã
æ¬çºæã«çšããããæŸå°æ§å
çŽ ã¯æŸå°ç·ïŒÎ±
ç·ãβç·ãγç·ãäžæ§åç·ãç·ãªã©ïŒãæŸå°ã
ããã®ã§ããã°ãã©ã®ãããªæ žçš®ã§ãã€ãŠããã
ãã代衚çãªãã®ãšããŠã¯ 32Pã 14Cã 35Sã
3Hã 125Iãªã©ãããã
ç·ãβç·ãγç·ãäžæ§åç·ãç·ãªã©ïŒãæŸå°ã
ããã®ã§ããã°ãã©ã®ãããªæ žçš®ã§ãã€ãŠããã
ãã代衚çãªãã®ãšããŠã¯ 32Pã 14Cã 35Sã
3Hã 125Iãªã©ãããã
æ¬çºæã«ãããŠåé¢å±éã®å¯Ÿè±¡ãšãããè©Šæã
ããªãã¡æŸå°æ§æšèãä»ãããçç©äœç±æ¥ã®ç©è³ª
ã®äŸãšããŠã¯ãèçœè³ªãæ žé žããããã®èªå°äœã
ãããã®å解ç©ã®ãããªé«ååç©è³ªãæããããš
ãã§ããããããã®ç©è³ªã«æŸå°æ§æšèãä»äžãã
æ¹æ³ã¯ãæ¢ã«è¯ãç¥ãããŠããããªããæ¬çºæã®
ãªãŒãã©ãžãªã°ã©ãã€ãŒçšæž¬å®ãããã®æž¬å®å¯Ÿè±¡
ãšãªãçç©äœç±æ¥ã®ç©è³ªã¯ãäžèšã®ãããªé«åå
ç©è³ªã«éå®ããããã®ã§ã¯ãªãã
ããªãã¡æŸå°æ§æšèãä»ãããçç©äœç±æ¥ã®ç©è³ª
ã®äŸãšããŠã¯ãèçœè³ªãæ žé žããããã®èªå°äœã
ãããã®å解ç©ã®ãããªé«ååç©è³ªãæããããš
ãã§ããããããã®ç©è³ªã«æŸå°æ§æšèãä»äžãã
æ¹æ³ã¯ãæ¢ã«è¯ãç¥ãããŠããããªããæ¬çºæã®
ãªãŒãã©ãžãªã°ã©ãã€ãŒçšæž¬å®ãããã®æž¬å®å¯Ÿè±¡
ãšãªãçç©äœç±æ¥ã®ç©è³ªã¯ãäžèšã®ãããªé«åå
ç©è³ªã«éå®ããããã®ã§ã¯ãªãã
ãŸããåèšã®ãããªåçš®ã®åé¢å±éçšæ¯æäœã
çšããåé¢å±éæ¹æ³ãæ¢ã«è¯ãç¥ãããŠãããã
ãã§ç¹ã«è§Šããããšã¯ããªãã
çšããåé¢å±éæ¹æ³ãæ¢ã«è¯ãç¥ãããŠãããã
ãã§ç¹ã«è§Šããããšã¯ããªãã
æ¬çºæã®ãªãŒãã©ãžãªã°ã©ãã€ãŒçšæž¬å®ããã
ã®äžæ¹ã®æ§æéšæã§ããæ¯æäœã®äžã«äžèšã®ãã
ã«ããŠåé¢å±éãããè©Šæãã圢æãããŠãããª
ãŒãã©ãžãªã°ã©ã ã¯ã次ã«ããã®æ¯æäœã«èç©æ§
èå äœã·ãŒãïŒæ¬çºæã®ãªãŒãã©ãžãªã°ã©ãã€ãŒ
çšæž¬å®ãããã®ããäžæ¹ã®æ§æéšæïŒãäžå®æé
éãåãããŠé²å æäœãå®æœããããšã«ãããã
ã®è©Šæã®æŸå°æ§æšèç©è³ªããæŸåºãããæŸå°ç·ã®
å°ãªããšãäžéšã該èå äœã·ãŒãã«åžåãããã
ãã«ããèå äœã·ãŒãã«ãªãŒãã©ãžãªã°ã©ã ãæŸ
å°ç·ãšãã«ã®ãŒã®èç©ãšããŠè»¢åãããã
ã®äžæ¹ã®æ§æéšæã§ããæ¯æäœã®äžã«äžèšã®ãã
ã«ããŠåé¢å±éãããè©Šæãã圢æãããŠãããª
ãŒãã©ãžãªã°ã©ã ã¯ã次ã«ããã®æ¯æäœã«èç©æ§
èå äœã·ãŒãïŒæ¬çºæã®ãªãŒãã©ãžãªã°ã©ãã€ãŒ
çšæž¬å®ãããã®ããäžæ¹ã®æ§æéšæïŒãäžå®æé
éãåãããŠé²å æäœãå®æœããããšã«ãããã
ã®è©Šæã®æŸå°æ§æšèç©è³ªããæŸåºãããæŸå°ç·ã®
å°ãªããšãäžéšã該èå äœã·ãŒãã«åžåãããã
ãã«ããèå äœã·ãŒãã«ãªãŒãã©ãžãªã°ã©ã ãæŸ
å°ç·ãšãã«ã®ãŒã®èç©ãšããŠè»¢åãããã
ãªããäžèšã®é²å
æäœã«ãããŠããªãŒãã©ãžãª
ã°ã©ã ãæããæ¯æäœã¯ããã®ãŸãŸããããã¯ä¹Ÿ
ç¥åŠçãåé¢å±éç©ã®åºå®åŠçãªã©ã®ä»»æã®åŠç
ãè¡ãªã€ãã®ã¡ã«èç©æ§èå äœã·ãŒããšéãåã
ãããããã«ãããã®é²å æäœãå®æœãããã
ã°ã©ã ãæããæ¯æäœã¯ããã®ãŸãŸããããã¯ä¹Ÿ
ç¥åŠçãåé¢å±éç©ã®åºå®åŠçãªã©ã®ä»»æã®åŠç
ãè¡ãªã€ãã®ã¡ã«èç©æ§èå äœã·ãŒããšéãåã
ãããããã«ãããã®é²å æäœãå®æœãããã
ãŸããé²å
æäœã«ãããŠãäžèšã®æ¯æäœãšèç©
æ§èå äœã·ãŒããšãéãåãããç¶æ ã¯ãéåžžã¯
æ¯æäœãšèç©æ§èå äœã·ãŒããšãå¯çãããããš
ã«ããå®çŸããããå¿ ãããããããå¯çããå¿
èŠã¯ãªããããããè¿æ¥ããç¶æ ã§é 眮ãããŠã
ãã°è¯ãã
æ§èå äœã·ãŒããšãéãåãããç¶æ ã¯ãéåžžã¯
æ¯æäœãšèç©æ§èå äœã·ãŒããšãå¯çãããããš
ã«ããå®çŸããããå¿ ãããããããå¯çããå¿
èŠã¯ãªããããããè¿æ¥ããç¶æ ã§é 眮ãããŠã
ãã°è¯ãã
ãŸããããããé²å
æéã¯ãè©Šæã«å«ãŸããæŸ
å°æ§æšèç©è³ªã®æŸå°èœã®åŒ·ãã該ç©è³ªã®æ¿åºŠãå¯
床ãªã©ãèç©æ§èå äœã·ãŒãã®æ床ãæ¯æäœãšè
ç©æ§èå äœã·ãŒããšã®äœçœ®é¢ä¿ãªã©ã«ããå€åã
ãããé²å æäœã¯äžå®æéãããšãã°ãæ°ç§çšåºŠ
以äžã¯å¿ èŠãšããããã ããæ¬çºæã«åŸã€ãŠæå
ææãšããŠèç©æ§èå äœã·ãŒããçšããå Žåã«
ã¯ãåŸæ¥ã®æŸå°ç·ãã€ã«ã ã䜿çšããå Žåã«å¿ èŠ
ãªé²å æéã«æ¯èŒããŠããã®é²å æéã¯å€§å¹ ã«ç
çž®ãããããŸããé²å ã«ããè©Šæããèç©æ§èå
äœã·ãŒãã«è»¢åèç©ãããè©Šæäžã®æŸå°æ§æšèç©
質ã®äœçœ®æ å ±ãèªã¿åºãæäœã«ãããŠã該èå äœ
ã·ãŒãã«èç©ãããŠãããšãã«ã®ãŒã®åŒ·ããå
åžãææã®æ å ±ãªã©ã«å¿ããŠåçš®ã®é»æ°çåŠçã
æœãããšã«ãããåŸãããäœçœ®æ å ±ã®ç¶æ ãå€ã
ãããšãå¯èœã§ãããããé²å æäœæã«ãããé²
å æéã®å³å¯ãªå¶åŸ¡ã¯ç¹ã«å¿ èŠãšã¯ããªãã
å°æ§æšèç©è³ªã®æŸå°èœã®åŒ·ãã該ç©è³ªã®æ¿åºŠãå¯
床ãªã©ãèç©æ§èå äœã·ãŒãã®æ床ãæ¯æäœãšè
ç©æ§èå äœã·ãŒããšã®äœçœ®é¢ä¿ãªã©ã«ããå€åã
ãããé²å æäœã¯äžå®æéãããšãã°ãæ°ç§çšåºŠ
以äžã¯å¿ èŠãšããããã ããæ¬çºæã«åŸã€ãŠæå
ææãšããŠèç©æ§èå äœã·ãŒããçšããå Žåã«
ã¯ãåŸæ¥ã®æŸå°ç·ãã€ã«ã ã䜿çšããå Žåã«å¿ èŠ
ãªé²å æéã«æ¯èŒããŠããã®é²å æéã¯å€§å¹ ã«ç
çž®ãããããŸããé²å ã«ããè©Šæããèç©æ§èå
äœã·ãŒãã«è»¢åèç©ãããè©Šæäžã®æŸå°æ§æšèç©
質ã®äœçœ®æ å ±ãèªã¿åºãæäœã«ãããŠã該èå äœ
ã·ãŒãã«èç©ãããŠãããšãã«ã®ãŒã®åŒ·ããå
åžãææã®æ å ±ãªã©ã«å¿ããŠåçš®ã®é»æ°çåŠçã
æœãããšã«ãããåŸãããäœçœ®æ å ±ã®ç¶æ ãå€ã
ãããšãå¯èœã§ãããããé²å æäœæã«ãããé²
å æéã®å³å¯ãªå¶åŸ¡ã¯ç¹ã«å¿ èŠãšã¯ããªãã
ãŸããé²å
æäœãå®æœãã枩床ã¯ç¹ã«å¶éã¯ãª
ãããæ¬çºæã®èç©æ§èå äœã·ãŒããå©çšãããª
ãŒãã©ãžãªã°ã©ãã€ãŒã¯ãç¹ã«10ã35âãªã©ã®ç°
å¢æž©åºŠã«ãŠå®æœããããšãå¯èœã§ããããã ãã
åŸæ¥ã®ãªãŒãã©ãžãªã°ã©ãã€ãŒã«ãããŠå©çšãã
ãŠããäœæž©ïŒããšãã°ãïŒâä»è¿ããããã¯ãã
以äžã®æž©åºŠïŒã«ãããŠé²å æäœãè¡ãªã€ãŠãã
ãã
ãããæ¬çºæã®èç©æ§èå äœã·ãŒããå©çšãããª
ãŒãã©ãžãªã°ã©ãã€ãŒã¯ãç¹ã«10ã35âãªã©ã®ç°
å¢æž©åºŠã«ãŠå®æœããããšãå¯èœã§ããããã ãã
åŸæ¥ã®ãªãŒãã©ãžãªã°ã©ãã€ãŒã«ãããŠå©çšãã
ãŠããäœæž©ïŒããšãã°ãïŒâä»è¿ããããã¯ãã
以äžã®æž©åºŠïŒã«ãããŠé²å æäœãè¡ãªã€ãŠãã
ãã
次ã«æ¬çºæã«ãããŠèç©æ§èå
äœã·ãŒãã«è»¢å
èç©ããããªãŒãã©ãžãªã°ã©ã ã瀺ãåé¢å±éã
ããç©è³ªã®äœçœ®æ å ±ãèªã¿åºãããã®æ¹æ³ã«ã€ã
ãŠãæ·»ä»å³é¢ã®ç¬¬ïŒå³ã«ç€ºããèªåºè£ 眮ïŒããã
ã¯èªåè£ çœ®ïŒã®äŸãåç §ããªããç¥è¿°ããã
èç©ããããªãŒãã©ãžãªã°ã©ã ã瀺ãåé¢å±éã
ããç©è³ªã®äœçœ®æ å ±ãèªã¿åºãããã®æ¹æ³ã«ã€ã
ãŠãæ·»ä»å³é¢ã®ç¬¬ïŒå³ã«ç€ºããèªåºè£ 眮ïŒããã
ã¯èªåè£ çœ®ïŒã®äŸãåç §ããªããç¥è¿°ããã
第ïŒå³ã¯ãèç©æ§èå
äœã·ãŒãïŒä»¥äžã«ãããŠ
ã¯ãèå äœã·ãŒããšç¥èšããããšãããïŒïŒã«è
ç©èšé²ãããŠããæŸå°æ§æšèç©è³ªã®äžæ¬¡å ããã
ã¯äºæ¬¡å çãªäœçœ®æ å ±ãä»®ã«èªã¿åºãããã®å èª
ã¿çšèªåºéšïŒãšãæŸå°æ§æšèç©è³ªã®äœçœ®æ å ±ãåº
åããããã«èå äœã·ãŒãïŒã«èç©èšé²ãããŠã
ãæŸå°ç·ç»åãèªã¿åºãæ©èœãæããæ¬èªã¿çšèª
åºéšïŒããæ§æãããèªåºè£ 眮ã®äŸã®æŠç¥å³ã瀺
ããŠããã
ã¯ãèå äœã·ãŒããšç¥èšããããšãããïŒïŒã«è
ç©èšé²ãããŠããæŸå°æ§æšèç©è³ªã®äžæ¬¡å ããã
ã¯äºæ¬¡å çãªäœçœ®æ å ±ãä»®ã«èªã¿åºãããã®å èª
ã¿çšèªåºéšïŒãšãæŸå°æ§æšèç©è³ªã®äœçœ®æ å ±ãåº
åããããã«èå äœã·ãŒãïŒã«èç©èšé²ãããŠã
ãæŸå°ç·ç»åãèªã¿åºãæ©èœãæããæ¬èªã¿çšèª
åºéšïŒããæ§æãããèªåºè£ 眮ã®äŸã®æŠç¥å³ã瀺
ããŠããã
å
èªã¿çšèªåºéšïŒã«ãããŠã¯æ¬¡ã®ãããªå
èªã¿
æäœãè¡ãªãããã
æäœãè¡ãªãããã
ã¬ãŒã¶ãŒå
æºïŒããçºçããã¬ãŒã¶ãŒå
ïŒã¯ã
ã€ã«ã¿ãŒïŒãééããããšã«ããããã®ã¬ãŒã¶ãŒ
å ïŒã«ããå±èµ·ã«å¿ããŠèå äœã·ãŒãïŒããçºç
ããèŒå°œçºå ã®æ³¢é·é åã«è©²åœããæ³¢é·é åã®éš
åãã«ãããããã次ãã§ã¬ãŒã¶ãŒå ã¯ãã¬ã«ã
ããã©ãŒçã®å åååšïŒã«ããåååŠçãããå¹³
é¢åå°é¡ïŒã«ããåå°ãããã®ã¡èå äœã·ãŒãïŒ
äžã«äžæ¬¡å çã«ååããŠå ¥å°ãããããã§çšãã
ã¬ãŒã¶ãŒå æºïŒã¯ããã®ã¬ãŒã¶ãŒå ïŒã®æ³¢é·é å
ããèå äœã·ãŒãïŒããçºããèŒå°œçºå ã®äž»èŠæ³¢
é·é åãšéè€ããªãããã«éžæãããã
ã€ã«ã¿ãŒïŒãééããããšã«ããããã®ã¬ãŒã¶ãŒ
å ïŒã«ããå±èµ·ã«å¿ããŠèå äœã·ãŒãïŒããçºç
ããèŒå°œçºå ã®æ³¢é·é åã«è©²åœããæ³¢é·é åã®éš
åãã«ãããããã次ãã§ã¬ãŒã¶ãŒå ã¯ãã¬ã«ã
ããã©ãŒçã®å åååšïŒã«ããåååŠçãããå¹³
é¢åå°é¡ïŒã«ããåå°ãããã®ã¡èå äœã·ãŒãïŒ
äžã«äžæ¬¡å çã«ååããŠå ¥å°ãããããã§çšãã
ã¬ãŒã¶ãŒå æºïŒã¯ããã®ã¬ãŒã¶ãŒå ïŒã®æ³¢é·é å
ããèå äœã·ãŒãïŒããçºããèŒå°œçºå ã®äž»èŠæ³¢
é·é åãšéè€ããªãããã«éžæãããã
èå
äœã·ãŒãïŒã¯ãäžèšã®ååã¬ãŒã¶ãŒå
ã®ç
§
å°äžã«ãããŠãç¢å°ïŒã®æ¹åã«ç§»éããããåŸã€
ãŠãèå äœã·ãŒãïŒã®å šé¢ã«ããã€ãŠååã¬ãŒã¶
ãŒå ãç §å°ãããããã«ãªãããªããã¬ãŒã¶ãŒå
æºïŒã®åºåãã¬ãŒã¶ãŒå ïŒã®ããŒã åŸãã¬ãŒã¶ãŒ
å ïŒã®èµ°æ»é床ãèå äœã·ãŒãïŒã®ç§»éé床ã«ã€
ããŠã¯ãå èªã¿æäœã®ã¬ãŒã¶ãŒå ïŒã®ãšãã«ã®ãŒ
ãæ¬èªã¿æäœã«çšãããããšãã«ã®ãŒãããå°ã
ããªãããã«èª¿æŽãããã
å°äžã«ãããŠãç¢å°ïŒã®æ¹åã«ç§»éããããåŸã€
ãŠãèå äœã·ãŒãïŒã®å šé¢ã«ããã€ãŠååã¬ãŒã¶
ãŒå ãç §å°ãããããã«ãªãããªããã¬ãŒã¶ãŒå
æºïŒã®åºåãã¬ãŒã¶ãŒå ïŒã®ããŒã åŸãã¬ãŒã¶ãŒ
å ïŒã®èµ°æ»é床ãèå äœã·ãŒãïŒã®ç§»éé床ã«ã€
ããŠã¯ãå èªã¿æäœã®ã¬ãŒã¶ãŒå ïŒã®ãšãã«ã®ãŒ
ãæ¬èªã¿æäœã«çšãããããšãã«ã®ãŒãããå°ã
ããªãããã«èª¿æŽãããã
èå
äœã·ãŒãïŒã¯ãäžèšã®ãããªã¬ãŒã¶ãŒå
ã®
ç §å°ãåãããšãèç©èšé²ãããŠããæŸå°ç·ãšã
ã«ã®ãŒã«æ¯äŸããå éã®èŒå°œçºå ã瀺ãããã®å
ã¯å èªã¿çšå°å æ§ã·ãŒãïŒïŒã«å ¥å°ããããã®å°
å æ§ã·ãŒãïŒïŒã¯ãã®å ¥å°é¢ãçŽç·ç¶ã§ãèå äœ
ã·ãŒãïŒäžã®èµ°æ»ç·ã«å¯Ÿåããããã«è¿æ¥ããŠé
眮ãããŠããããã®å°åºé¢ã¯åç°ã圢æããããª
ããã«ãªã©ã®å æ€åºåšïŒïŒã®åå é¢ã«é£çµ¡ããŠã
ãããã®å°å æ§ã·ãŒãïŒïŒã¯ãããšãã°ã¢ã¯ãªã«
ç³»åææš¹èãªã©ã®éæãªç±å¯å¡æ§æš¹èã·ãŒããå
å·¥ããŠã€ãããããã®ã§ãå ¥å°é¢ããå ¥å°ããå
ããã®å éšã«ãããŠå šåå°ããªããå°åºé¢ãžäŒé
ãããããã«æ§æãããŠãããèå äœã·ãŒãïŒã
ãã®èŒå°œçºå ã¯ãã®å°å æ§ã·ãŒãïŒïŒå ãå°ãã
ãŠå°åºé¢ã«å°éãããã®å°åºé¢ããå°åºãããŠå
æ€åºåšïŒïŒã«åå ãããã
ç §å°ãåãããšãèç©èšé²ãããŠããæŸå°ç·ãšã
ã«ã®ãŒã«æ¯äŸããå éã®èŒå°œçºå ã瀺ãããã®å
ã¯å èªã¿çšå°å æ§ã·ãŒãïŒïŒã«å ¥å°ããããã®å°
å æ§ã·ãŒãïŒïŒã¯ãã®å ¥å°é¢ãçŽç·ç¶ã§ãèå äœ
ã·ãŒãïŒäžã®èµ°æ»ç·ã«å¯Ÿåããããã«è¿æ¥ããŠé
眮ãããŠããããã®å°åºé¢ã¯åç°ã圢æããããª
ããã«ãªã©ã®å æ€åºåšïŒïŒã®åå é¢ã«é£çµ¡ããŠã
ãããã®å°å æ§ã·ãŒãïŒïŒã¯ãããšãã°ã¢ã¯ãªã«
ç³»åææš¹èãªã©ã®éæãªç±å¯å¡æ§æš¹èã·ãŒããå
å·¥ããŠã€ãããããã®ã§ãå ¥å°é¢ããå ¥å°ããå
ããã®å éšã«ãããŠå šåå°ããªããå°åºé¢ãžäŒé
ãããããã«æ§æãããŠãããèå äœã·ãŒãïŒã
ãã®èŒå°œçºå ã¯ãã®å°å æ§ã·ãŒãïŒïŒå ãå°ãã
ãŠå°åºé¢ã«å°éãããã®å°åºé¢ããå°åºãããŠå
æ€åºåšïŒïŒã«åå ãããã
ãªããå°å
æ§ã·ãŒãã®å¥œãŸãã圢ç¶ãæ質çã¯
ç¹éæ55â87970å·å ¬å ±ãå56â11397å·å ¬å ±çã«
é瀺ãããã
ç¹éæ55â87970å·å ¬å ±ãå56â11397å·å ¬å ±çã«
é瀺ãããã
å
æ€åºåšïŒïŒã®åå
é¢ã«ã¯ãèŒå°œçºå
ã®æ³¢é·é
åã®å ã®ã¿ãééããå±èµ·å ïŒã¬ãŒã¶ãŒå ïŒã®æ³¢
é·é åã®å ãã«ãããããã€ã«ã¿ãŒã貌çããã
èŒå°œçºå ã®ã¿ãæ€åºãããããã«ãããŠãããå
æ€åºåšïŒïŒã«ããæ€åºãããèŒå°œçºå ã¯é»æ°ä¿¡å·
ã«å€æãããããã«å¢å¹ åšïŒïŒã«ããå¢å¹ ããåº
åããããå¢å¹ åšïŒïŒããåºåãããèç©èšé²æ
å ±ã¯ãæ¬èªã¿çšèªåºéšïŒã®å¶åŸ¡åè·¯ïŒïŒã«å ¥åã
ãããå¶åŸ¡åè·¯ïŒïŒã¯ãåŸãããèç©èšé²æ å ±ã«
å¿ããŠãæ¿åºŠããã³ã³ã³ãã©ã¹ããæãåäžã§ã
ã€èŠ³å¯èªåœ±æ§èœã®åªããç»åãåŸãããããã«ã
å¢å¹ çèšå®å€ïœãåé²ã¹ã±ãŒã«ãã¢ã¯ã¿ãŒïœãã
ãã³ãåçç»ååŠçæ¡ä»¶èšå®å€ïœãåºåããã
åã®å ã®ã¿ãééããå±èµ·å ïŒã¬ãŒã¶ãŒå ïŒã®æ³¢
é·é åã®å ãã«ãããããã€ã«ã¿ãŒã貌çããã
èŒå°œçºå ã®ã¿ãæ€åºãããããã«ãããŠãããå
æ€åºåšïŒïŒã«ããæ€åºãããèŒå°œçºå ã¯é»æ°ä¿¡å·
ã«å€æãããããã«å¢å¹ åšïŒïŒã«ããå¢å¹ ããåº
åããããå¢å¹ åšïŒïŒããåºåãããèç©èšé²æ
å ±ã¯ãæ¬èªã¿çšèªåºéšïŒã®å¶åŸ¡åè·¯ïŒïŒã«å ¥åã
ãããå¶åŸ¡åè·¯ïŒïŒã¯ãåŸãããèç©èšé²æ å ±ã«
å¿ããŠãæ¿åºŠããã³ã³ã³ãã©ã¹ããæãåäžã§ã
ã€èŠ³å¯èªåœ±æ§èœã®åªããç»åãåŸãããããã«ã
å¢å¹ çèšå®å€ïœãåé²ã¹ã±ãŒã«ãã¢ã¯ã¿ãŒïœãã
ãã³ãåçç»ååŠçæ¡ä»¶èšå®å€ïœãåºåããã
以äžã®ããã«ããŠå
èªã¿æäœãçµäºããèå
äœ
ã·ãŒãïŒã¯æ¬èªã¿çšèªåºéšïŒãžç§»éãããã
ã·ãŒãïŒã¯æ¬èªã¿çšèªåºéšïŒãžç§»éãããã
æ¬èªã¿çšèªåºéšïŒã«ãããŠã¯æ¬¡ã®ãããªæ¬èªã¿
æäœãè¡ãªãããã
æäœãè¡ãªãããã
æ¬èªã¿çšã¬ãŒã¶ãŒå
æºïŒïŒããçºããããã¬ãŒ
ã¶ãŒå ïŒïŒã¯ãåè¿°ã®ãã€ã«ã¿ãŒïŒãšåæ§ãªæ©èœ
ãæãããã€ã«ã¿ãŒïŒïŒãééããã®ã¡ããŒã ã»
ãšã¯ã¹ãã³ããŒïŒïŒã«ããããŒã åŸã®å€§ãããå³
å¯ã«èª¿æŽãããã次ãã§ã¬ãŒã¶ãŒå ã¯ãã¬ã«ãã
ãã©ãŒçã®å åååšïŒïŒã«ããåååŠçãããå¹³
é¢åå°é¡ïŒïŒã«ããåå°ãããã®ã¡èå äœã·ãŒã
ïŒäžã«äžæ¬¡å çã«ååããŠå ¥å°ããããªããå å
ååšïŒïŒãšå¹³é¢åå°é¡ïŒïŒãšã®éã«ã¯fΞã¬ã³ãºïŒ
ïŒãé 眮ãããèå äœã·ãŒãïŒã®äžãååã¬ãŒã¶
ãŒå ãèµ°æ»ããå Žåã«ãåžžã«åäžãªããŒã é床ã
ç¶æããããã«ãããŠããã
ã¶ãŒå ïŒïŒã¯ãåè¿°ã®ãã€ã«ã¿ãŒïŒãšåæ§ãªæ©èœ
ãæãããã€ã«ã¿ãŒïŒïŒãééããã®ã¡ããŒã ã»
ãšã¯ã¹ãã³ããŒïŒïŒã«ããããŒã åŸã®å€§ãããå³
å¯ã«èª¿æŽãããã次ãã§ã¬ãŒã¶ãŒå ã¯ãã¬ã«ãã
ãã©ãŒçã®å åååšïŒïŒã«ããåååŠçãããå¹³
é¢åå°é¡ïŒïŒã«ããåå°ãããã®ã¡èå äœã·ãŒã
ïŒäžã«äžæ¬¡å çã«ååããŠå ¥å°ããããªããå å
ååšïŒïŒãšå¹³é¢åå°é¡ïŒïŒãšã®éã«ã¯fΞã¬ã³ãºïŒ
ïŒãé 眮ãããèå äœã·ãŒãïŒã®äžãååã¬ãŒã¶
ãŒå ãèµ°æ»ããå Žåã«ãåžžã«åäžãªããŒã é床ã
ç¶æããããã«ãããŠããã
èå
äœã·ãŒãïŒã¯ãäžèšã®ååã¬ãŒã¶ãŒå
ã®ç
§
å°äžã«ãããŠãç¢å°ïŒïŒã®æ¹åã«ç§»éããããåŸ
ã€ãŠãå èªã¿æäœã«ããããšåæ§ã«èå äœã·ãŒã
ïŒã®å šé¢ã«ããã€ãŠååã¬ãŒã¶ãŒå ãç §å°ããã
ããã«ãªãã
å°äžã«ãããŠãç¢å°ïŒïŒã®æ¹åã«ç§»éããããåŸ
ã€ãŠãå èªã¿æäœã«ããããšåæ§ã«èå äœã·ãŒã
ïŒã®å šé¢ã«ããã€ãŠååã¬ãŒã¶ãŒå ãç §å°ããã
ããã«ãªãã
èå
äœã·ãŒãïŒã¯ãäžèšã®ããã«ããŠã¬ãŒã¶ãŒ
å ã®ç §å°ãåãããšãå èªã¿æäœã«ããããšåæ§
ã«ãèç©èšé²ãããŠããæŸå°ç·ãšãã«ã®ãŒã«æ¯äŸ
ããå éã®èŒå°œçºå ãçºãããã®å ã¯æ¬èªã¿çšå°
å æ§ã·ãŒãïŒïŒã«å ¥å°ããããã®æ¬èªã¿çšå°å æ§
ã·ãŒãïŒïŒã¯å èªã¿çšå°å æ§ã·ãŒãïŒïŒãšåæ§ã®
æ質ãæ§é ãæããŠãããæ¬èªã¿çšå°å æ§ã·ãŒã
ïŒïŒã®å éšãå šåå°ãç¹°è¿ãã€ã€å°ãããèŒå°œçº
å ã¯ãã®å°åºé¢ããå°åºãããŠãå æ€åºåšïŒïŒã«
åå ãããããªããå æ€åºåšïŒïŒã®åå é¢ã«ã¯èŒ
å°œçºå ã®æ³¢é·é åã®ã¿ãéžæçã«ééãããã€ã«
ã¿ãŒã貌çãããå æ€åºåšïŒïŒãèŒå°œçºå ã®ã¿ã
æ€åºããããã«ãããŠããã
å ã®ç §å°ãåãããšãå èªã¿æäœã«ããããšåæ§
ã«ãèç©èšé²ãããŠããæŸå°ç·ãšãã«ã®ãŒã«æ¯äŸ
ããå éã®èŒå°œçºå ãçºãããã®å ã¯æ¬èªã¿çšå°
å æ§ã·ãŒãïŒïŒã«å ¥å°ããããã®æ¬èªã¿çšå°å æ§
ã·ãŒãïŒïŒã¯å èªã¿çšå°å æ§ã·ãŒãïŒïŒãšåæ§ã®
æ質ãæ§é ãæããŠãããæ¬èªã¿çšå°å æ§ã·ãŒã
ïŒïŒã®å éšãå šåå°ãç¹°è¿ãã€ã€å°ãããèŒå°œçº
å ã¯ãã®å°åºé¢ããå°åºãããŠãå æ€åºåšïŒïŒã«
åå ãããããªããå æ€åºåšïŒïŒã®åå é¢ã«ã¯èŒ
å°œçºå ã®æ³¢é·é åã®ã¿ãéžæçã«ééãããã€ã«
ã¿ãŒã貌çãããå æ€åºåšïŒïŒãèŒå°œçºå ã®ã¿ã
æ€åºããããã«ãããŠããã
å
æ€åºåšïŒïŒã«ããæ€åºãããèŒå°œçºå
ã¯é»æ°
ä¿¡å·ã«å€æãããåèšã®å¢å¹ çèšå®å€ïœã«åŸã€ãŠ
æ床èšå®ãããå¢å¹ åšïŒïŒã«ãããŠé©æ£ã¬ãã«ã®
é»æ°ä¿¡å·ã«å¢å¹ ãããã®ã¡ãïŒïŒ€å€æåšïŒïŒã«
å ¥åããããïŒïŒ€å€æåšïŒïŒã¯ãåé²ã¹ã±ãŒã«
ãã¢ã¯ã¿ãŒèšå®å€ïœã«åŸãä¿¡å·å€åå¹ ã«é©ããã¹
ã±ãŒã«ãã¢ã¯ã¿ãŒã§ããžã¿ã«ä¿¡å·ã«å€æãããä¿¡
å·åŠçåè·¯ïŒïŒã«å ¥åããããä¿¡å·åŠçåè·¯ïŒïŒ
ã§ã¯ãåçç»ååŠçæ¡ä»¶èšå®å€ïœã«åºã¥ããŠãæ¿
床ããã³ã³ã³ãã©ã¹ããé©æ£ã§èŠ³å¯èªåœ±æ§èœã®åª
ããå¯èŠç»åãåŸãããããã«ä¿¡å·åŠçãè¡ãªã
ãã次ãã§å¿ èŠã«ããç£æ°ããŒããªã©ã®ä¿åæ段
ãä»ããŠãèšé²è£ 眮ïŒå³ç€ºãªãïŒãžäŒéãããã
ä¿¡å·ã«å€æãããåèšã®å¢å¹ çèšå®å€ïœã«åŸã€ãŠ
æ床èšå®ãããå¢å¹ åšïŒïŒã«ãããŠé©æ£ã¬ãã«ã®
é»æ°ä¿¡å·ã«å¢å¹ ãããã®ã¡ãïŒïŒ€å€æåšïŒïŒã«
å ¥åããããïŒïŒ€å€æåšïŒïŒã¯ãåé²ã¹ã±ãŒã«
ãã¢ã¯ã¿ãŒèšå®å€ïœã«åŸãä¿¡å·å€åå¹ ã«é©ããã¹
ã±ãŒã«ãã¢ã¯ã¿ãŒã§ããžã¿ã«ä¿¡å·ã«å€æãããä¿¡
å·åŠçåè·¯ïŒïŒã«å ¥åããããä¿¡å·åŠçåè·¯ïŒïŒ
ã§ã¯ãåçç»ååŠçæ¡ä»¶èšå®å€ïœã«åºã¥ããŠãæ¿
床ããã³ã³ã³ãã©ã¹ããé©æ£ã§èŠ³å¯èªåœ±æ§èœã®åª
ããå¯èŠç»åãåŸãããããã«ä¿¡å·åŠçãè¡ãªã
ãã次ãã§å¿ èŠã«ããç£æ°ããŒããªã©ã®ä¿åæ段
ãä»ããŠãèšé²è£ 眮ïŒå³ç€ºãªãïŒãžäŒéãããã
èšé²è£
眮ãšããŠã¯ãããšãã°ãæå
ææäžãã¬
ãŒã¶ãŒå çã§èµ°æ»ããŠå åŠçã«èšé²ãããã®ã
CRTçã«é»åçã«è¡šç€ºãããã®ãCRTçã«è¡šç€º
ãããæŸå°ç·ç»åããããªã»ããªã³ã¿ãŒçã«èšé²
ãããã®ãç±ç·ãçšããŠæç±èšé²ææäžã«èšé²ã
ããã®ãªã©çš®ã ã®åçã«åºã¥ããèšé²è£ 眮ãçšã
ãããšãã§ããã
ãŒã¶ãŒå çã§èµ°æ»ããŠå åŠçã«èšé²ãããã®ã
CRTçã«é»åçã«è¡šç€ºãããã®ãCRTçã«è¡šç€º
ãããæŸå°ç·ç»åããããªã»ããªã³ã¿ãŒçã«èšé²
ãããã®ãç±ç·ãçšããŠæç±èšé²ææäžã«èšé²ã
ããã®ãªã©çš®ã ã®åçã«åºã¥ããèšé²è£ 眮ãçšã
ãããšãã§ããã
ãã ããèšé²è£
眮ã¯äžèšã®ããã«å¯èŠç»ååã
ããã®ã«éããããã®ã§ã¯ãªããåè¿°ããããã«
è©Šæäžã®æŸå°æ§æšèç©è³ªã®äžæ¬¡å çãããã¯äºæ¬¡
å çãªäœçœ®æ å ±ããããšãã°æ°ååãããã¯èšå·
åãããªã©ããŠèšé²ããããšãã§ããã
ããã®ã«éããããã®ã§ã¯ãªããåè¿°ããããã«
è©Šæäžã®æŸå°æ§æšèç©è³ªã®äžæ¬¡å çãããã¯äºæ¬¡
å çãªäœçœ®æ å ±ããããšãã°æ°ååãããã¯èšå·
åãããªã©ããŠèšé²ããããšãã§ããã
ãªããæ¬çºæã«ãããèç©æ§èå
äœã·ãŒãã«è»¢
åèç©ãããè©Šæäžã®æŸå°æ§æšèç©è³ªã®äœçœ®æ å ±
ãèªã¿åºãããã®æ¹æ³ã«ã€ããŠãäžèšã«ãããŠã¯
å èªã¿æäœãšæ¬èªã¿æäœãšãããªãèªåºãæäœã
説æããããæ¬çºæã«ãããŠå©çšããããšãã§ã
ãèªåºãæäœã¯ãäžèšã®äŸã«éããããã®ã§ã¯ãª
ããããšãã°ãè©Šæäžã®æŸå°æ§ç©è³ªã®å«æéãã
ã³ããã®è©Šæã«ã€ããŠã®èç©æ§èå äœã·ãŒãã®é²
å æéãäºãããã€ãŠããã°ãäžèšã®äŸã«ãããŠ
å èªã¿æäœãçç¥ããããšãã§ããã
åèç©ãããè©Šæäžã®æŸå°æ§æšèç©è³ªã®äœçœ®æ å ±
ãèªã¿åºãããã®æ¹æ³ã«ã€ããŠãäžèšã«ãããŠã¯
å èªã¿æäœãšæ¬èªã¿æäœãšãããªãèªåºãæäœã
説æããããæ¬çºæã«ãããŠå©çšããããšãã§ã
ãèªåºãæäœã¯ãäžèšã®äŸã«éããããã®ã§ã¯ãª
ããããšãã°ãè©Šæäžã®æŸå°æ§ç©è³ªã®å«æéãã
ã³ããã®è©Šæã«ã€ããŠã®èç©æ§èå äœã·ãŒãã®é²
å æéãäºãããã€ãŠããã°ãäžèšã®äŸã«ãããŠ
å èªã¿æäœãçç¥ããããšãã§ããã
ãŸããæ¬çºæã«ãããèç©æ§èå
äœã·ãŒãã«è»¢
åèç©ãããè©Šæäžã®æŸå°æ§æšèç©è³ªã®äœçœ®æ å ±
ãèªã¿åºãããã®æ¹æ³ãšããŠã¯ãäžèšã«äŸç€ºãã
以å€ã®é©åœãªæ¹æ³ãå©çšããããšãåœç¶å¯èœã§ã
ãã
åèç©ãããè©Šæäžã®æŸå°æ§æšèç©è³ªã®äœçœ®æ å ±
ãèªã¿åºãããã®æ¹æ³ãšããŠã¯ãäžèšã«äŸç€ºãã
以å€ã®é©åœãªæ¹æ³ãå©çšããããšãåœç¶å¯èœã§ã
ãã
ãªããæ¬çºæã«ãããŠè©Šæäžã®æŸå°æ§æšèç©è³ª
ã®ãäœçœ®æ å ±ããšã¯ãè©Šæäžã«ãããæŸå°æ§æšè
ç©è³ªãããã¯ãã®éåäœã®äœçœ®ãäžå¿ãšããåçš®
ã®æ å ±ãããšãã°ãè©Šæäžã«ååšããæŸå°æ§ç©è³ª
ã®éåäœã®ååšäœçœ®ãšåœ¢ç¶ããã®äœçœ®ã«ãããæŸ
å°æ§ç©è³ªã®æ¿åºŠãååžãªã©ãããªãæ å ±ã®äžã€ã
ããã¯ä»»æã®çµåããšããŠåŸãããåçš®ã®æ å ±ã
æå³ããã
ã®ãäœçœ®æ å ±ããšã¯ãè©Šæäžã«ãããæŸå°æ§æšè
ç©è³ªãããã¯ãã®éåäœã®äœçœ®ãäžå¿ãšããåçš®
ã®æ å ±ãããšãã°ãè©Šæäžã«ååšããæŸå°æ§ç©è³ª
ã®éåäœã®ååšäœçœ®ãšåœ¢ç¶ããã®äœçœ®ã«ãããæŸ
å°æ§ç©è³ªã®æ¿åºŠãååžãªã©ãããªãæ å ±ã®äžã€ã
ããã¯ä»»æã®çµåããšããŠåŸãããåçš®ã®æ å ±ã
æå³ããã
次ã«æ¬çºæã®æž¬å®çšããããçšãããªãŒãã©ãž
ãªã°ã©ãã€ãŒã®å®æœæ æ§ããåè¿°ã®ãããµã ã»ã®
ã«ããŒãæ³ãå©çšããDNAã®å¡©åºé å決å®æ³ã®
ã®åææäœãäŸã«ããŠèšèŒããã
ãªã°ã©ãã€ãŒã®å®æœæ æ§ããåè¿°ã®ãããµã ã»ã®
ã«ããŒãæ³ãå©çšããDNAã®å¡©åºé å決å®æ³ã®
ã®åææäœãäŸã«ããŠèšèŒããã
ãŸãã以äžã®å®æœäŸã«ãããŠäœ¿çšãã枬å®çšã
ããã¯ãåžžæ³ã«ãã調補ããïŒïŒ ããªã¢ã¯ãªã«ã¢
ããïŒæ¶æ©å€çïŒïŒïŒ ïŒã®ã¹ã©ãã²ã«ïŒ1.5mmÃ
200mmÃ200mmïŒãããªãé»æ°æ³³åçšæ¯æäœããšäž
èšã®æ¹æ³ã«ãã調補ããèç©æ§èå äœã·ãŒããšã
ããªããã®ã§ããã
ããã¯ãåžžæ³ã«ãã調補ããïŒïŒ ããªã¢ã¯ãªã«ã¢
ããïŒæ¶æ©å€çïŒïŒïŒ ïŒã®ã¹ã©ãã²ã«ïŒ1.5mmÃ
200mmÃ200mmïŒãããªãé»æ°æ³³åçšæ¯æäœããšäž
èšã®æ¹æ³ã«ãã調補ããèç©æ§èå äœã·ãŒããšã
ããªããã®ã§ããã
èŒå°œæ§ã®ãŠãŒãããŠã 賊掻åŒåèåããªãŠã è
å äœïŒBaFBrïŒEuïŒã®ç²åãšç·ç¶ããªãšã¹ãã«
æš¹èãšã®æ··åç©ã«ã¡ãã«ãšãã«ã±ãã³ãæ·»å ãã
ããã«ç¡å床11.5ïŒ ã®ãããã»ã«ããŒã¹ãæ·»å ã
ãŠèå äœç²åãåæ£ç¶æ ã§å«æããåæ£æ¶²ã調補
ããã次ã«ããã®åæ£æ¶²ã«çé žããªã¯ã¬ãžã«ãïœ
âãã¿ããŒã«ããããŠã¡ãã«ãšãã«ã±ãã³ãæ·»å
ããã®ã¡ããããã©ãããµãŒãçšããŠå åã«æ¹æ
æ··åããŠãèå äœç²åãåäžã«åæ£ãããã€ç²åºŠ
ã25ã35PSïŒ25âïŒã®å¡åžæ¶²ã調補ããã
å äœïŒBaFBrïŒEuïŒã®ç²åãšç·ç¶ããªãšã¹ãã«
æš¹èãšã®æ··åç©ã«ã¡ãã«ãšãã«ã±ãã³ãæ·»å ãã
ããã«ç¡å床11.5ïŒ ã®ãããã»ã«ããŒã¹ãæ·»å ã
ãŠèå äœç²åãåæ£ç¶æ ã§å«æããåæ£æ¶²ã調補
ããã次ã«ããã®åæ£æ¶²ã«çé žããªã¯ã¬ãžã«ãïœ
âãã¿ããŒã«ããããŠã¡ãã«ãšãã«ã±ãã³ãæ·»å
ããã®ã¡ããããã©ãããµãŒãçšããŠå åã«æ¹æ
æ··åããŠãèå äœç²åãåäžã«åæ£ãããã€ç²åºŠ
ã25ã35PSïŒ25âïŒã®å¡åžæ¶²ã調補ããã
次ã«ãã¬ã©ã¹æ¿äžã«æ°Žå¹³ã«çœ®ããã«ãŒãã³ãã©
ãã¯ç·Žã蟌ã¿ããªãšãã¬ã³ãã¬ãã¿ã¬ãŒãã·ãŒã
ïŒæ¯æäœãåã¿ïŒ250ÎŒmïŒã®äžã«å¡åžæ¶²ããã¯ã¿
ãã¬ãŒããçšããŠåäžã«å¡åžããããããŠå¡åžåŸ
ã«ãå¡èã圢æãããæ¯æäœã也ç¥åšå ã«å ¥ãã
ãã®ä¹Ÿç¥åšå éšã®æž©åºŠã25âãã100âã«åŸã ã«
äžæãããŠãå¡èã®ä¹Ÿç¥ãè¡ãªã€ãããã®ããã«
ããŠãæ¯æäœäžã«å±€åã300ÎŒmã®èå äœå±€ã圢æ
ããã
ãã¯ç·Žã蟌ã¿ããªãšãã¬ã³ãã¬ãã¿ã¬ãŒãã·ãŒã
ïŒæ¯æäœãåã¿ïŒ250ÎŒmïŒã®äžã«å¡åžæ¶²ããã¯ã¿
ãã¬ãŒããçšããŠåäžã«å¡åžããããããŠå¡åžåŸ
ã«ãå¡èã圢æãããæ¯æäœã也ç¥åšå ã«å ¥ãã
ãã®ä¹Ÿç¥åšå éšã®æž©åºŠã25âãã100âã«åŸã ã«
äžæãããŠãå¡èã®ä¹Ÿç¥ãè¡ãªã€ãããã®ããã«
ããŠãæ¯æäœäžã«å±€åã300ÎŒmã®èå äœå±€ã圢æ
ããã
ãããŠããã®èå
äœå±€ã®äžã«ãéæãªããªãšã
ã¬ã³ãã¬ãã¿ã¬ãŒããã€ã«ã ïŒåã¿ïŒ12ÎŒmïŒã®
çé¢ã«ããªãšã¹ãã«ç³»æ¥çå€ãä»äžããã®ã¡ãæ¥
çå€å±€åŽãäžã«åããŠçœ®ããŠæ¥çããããšã«ã
ããä¿è·èã圢æããæ¯æäœãèå äœå±€ãããã³
ä¿è·èããæ§æãããèç©æ§èå äœã·ãŒãã調補
ããã
ã¬ã³ãã¬ãã¿ã¬ãŒããã€ã«ã ïŒåã¿ïŒ12ÎŒmïŒã®
çé¢ã«ããªãšã¹ãã«ç³»æ¥çå€ãä»äžããã®ã¡ãæ¥
çå€å±€åŽãäžã«åããŠçœ®ããŠæ¥çããããšã«ã
ããä¿è·èã圢æããæ¯æäœãèå äœå±€ãããã³
ä¿è·èããæ§æãããèç©æ§èå äœã·ãŒãã調補
ããã
å®æœäŸ ïŒ
å¡©åºé
å決å®ã®å¯Ÿè±¡ãšãªãDNAã®åé¢ããã³
æŸå°æ§æšèå åžžæ³ã«ããå€§è žèãã©ã¹ããDNAïŒpBR322ïŒ
ãå¶éé µçŽ Hindâã«ããåæããã®ã¡ã5â²â
æ«ç«¯ã 32Pã§æšèããŠãäºæ¬éDNAïŒ 32Pæšè
ç©ïŒ1ÎŒgãåŸãã
æŸå°æ§æšèå åžžæ³ã«ããå€§è žèãã©ã¹ããDNAïŒpBR322ïŒ
ãå¶éé µçŽ Hindâã«ããåæããã®ã¡ã5â²â
æ«ç«¯ã 32Pã§æšèããŠãäºæ¬éDNAïŒ 32Pæšè
ç©ïŒ1ÎŒgãåŸãã
å¥ã«èª¿è£œãã5mMã®å¡©åãã°ãã·ãŠã ããã³
1mMã®ãžããªã¹ã¬ã€ããŒã«ãå«ã20mMã®ããª
ã¹ãããªã¹ïŒããããã·ã¡ãã«ïŒã¢ããã¡ã¿
ã³ãã»å¡©é žç·©è¡æ¶²ïŒPH7.4ïŒ20ÎŒlã«äžèšã®äºæ¬é
DNA1ÎŒgãšå¶éé µçŽ HaeâçŽïŒåäœãå ãã
37âã«ãŠïŒæéã®ç¹ç°çå解åå¿ãè¡ãªããäžèš
æçã®å解çæç©ãå«ãå解混åç©æº¶æ¶²ãåŸãã
1mMã®ãžããªã¹ã¬ã€ããŒã«ãå«ã20mMã®ããª
ã¹ãããªã¹ïŒããããã·ã¡ãã«ïŒã¢ããã¡ã¿
ã³ãã»å¡©é žç·©è¡æ¶²ïŒPH7.4ïŒ20ÎŒlã«äžèšã®äºæ¬é
DNA1ÎŒgãšå¶éé µçŽ HaeâçŽïŒåäœãå ãã
37âã«ãŠïŒæéã®ç¹ç°çå解åå¿ãè¡ãªããäžèš
æçã®å解çæç©ãå«ãå解混åç©æº¶æ¶²ãåŸãã
äžèšã®å解混åç©æº¶æ¶²ãè©ŠæãšããŠãã¹ã©ãã²
ã«æ¯æäœãçšãããã€1mMã®EDTAãå«ã
50mAã®ããªã¹ã»ããŠé žç·©è¡æ¶²ïŒPH8.3ïŒãé»æ¥µæ¶²
ãšããŠãé»å§500Vã«ãŠé»æ°æ³³åæäœãå®æœããã
è©Šæã«äºãå ããŠãããããŒã«ãŒè²çŽ ãã²ã«ã®äž
端éšã«å°éããæç¹ã«ãŠæ³³åãåæ¢ããã座æšè»ž
ã®åç¹ãšãªãäœçœ®ã« 32På«æã€ã³ã¯ã§å°ãä»ã
ãã
ã«æ¯æäœãçšãããã€1mMã®EDTAãå«ã
50mAã®ããªã¹ã»ããŠé žç·©è¡æ¶²ïŒPH8.3ïŒãé»æ¥µæ¶²
ãšããŠãé»å§500Vã«ãŠé»æ°æ³³åæäœãå®æœããã
è©Šæã«äºãå ããŠãããããŒã«ãŒè²çŽ ãã²ã«ã®äž
端éšã«å°éããæç¹ã«ãŠæ³³åãåæ¢ããã座æšè»ž
ã®åç¹ãšãªãäœçœ®ã« 32På«æã€ã³ã¯ã§å°ãä»ã
ãã
äžèšã®ã²ã«ãšèç©æ§èå
äœã·ãŒããéãåãã
ãŠã宀枩ïŒçŽ25âïŒã«ãŠïŒåéä¿æããŠé²å æäœ
ãè¡ãªã€ãã®ã¡ããã®èç©æ§èå äœã·ãŒãã第ïŒ
å³ã«ç€ºããããªèªåºè£ 眮ã«å°å ¥ãã 32På«æã€ã³
ã¯ã§å°ãä»ããäœçœ®ã座æšè»žã®åç¹ãšããŠã 32P
æšèæçã®å解çæç©ã®æ³³åäœçœ®ã瀺ãäœçœ®æ å ±
ãèªåºããã次ãã§ããã®äœçœ®æ å ±ã«åŸãã 32P
æšèãæããå解çæç©ãå«ãã²ã«éšåãèãã«
ããœãªãçšããŠååºããŠããããè©Šéšç®¡ã«ç§»ã
ãã
ãŠã宀枩ïŒçŽ25âïŒã«ãŠïŒåéä¿æããŠé²å æäœ
ãè¡ãªã€ãã®ã¡ããã®èç©æ§èå äœã·ãŒãã第ïŒ
å³ã«ç€ºããããªèªåºè£ 眮ã«å°å ¥ãã 32På«æã€ã³
ã¯ã§å°ãä»ããäœçœ®ã座æšè»žã®åç¹ãšããŠã 32P
æšèæçã®å解çæç©ã®æ³³åäœçœ®ã瀺ãäœçœ®æ å ±
ãèªåºããã次ãã§ããã®äœçœ®æ å ±ã«åŸãã 32P
æšèãæããå解çæç©ãå«ãã²ã«éšåãèãã«
ããœãªãçšããŠååºããŠããããè©Šéšç®¡ã«ç§»ã
ãã
ãªãã確èªã®ããã«ãäžèšã®äžéšååºãæäœã
è¡ãªã€ãæ®ãã®ã²ã«ãåæ§ã«ããŠèç©æ§èå äœã·
ãŒããšéãåãããã®ã¡ãèªåºè£ 眮ã«ãŠ 32Pæšè
ãæããå解çæç©ã®æ®åã®æç¡ã調ã¹ããšã
ãã 32Pæšèãæããå解çæç©ã®å šéãåãå»
ãããŠããããšãããã€ããããªãã¡ãäžèšã®è
ç©æ§èå äœã·ãŒããä»ããŠåŸã 32Pæšèãæãã
å解çæç©ã®äœçœ®æ å ±ã¯ç²ŸåºŠã®é«ããã®ã§ããã
ãšã確èªãããã
è¡ãªã€ãæ®ãã®ã²ã«ãåæ§ã«ããŠèç©æ§èå äœã·
ãŒããšéãåãããã®ã¡ãèªåºè£ 眮ã«ãŠ 32Pæšè
ãæããå解çæç©ã®æ®åã®æç¡ã調ã¹ããšã
ãã 32Pæšèãæããå解çæç©ã®å šéãåãå»
ãããŠããããšãããã€ããããªãã¡ãäžèšã®è
ç©æ§èå äœã·ãŒããä»ããŠåŸã 32Pæšèãæãã
å解çæç©ã®äœçœ®æ å ±ã¯ç²ŸåºŠã®é«ããã®ã§ããã
ãšã確èªãããã
第ïŒå³ã¯ãæ¬çºæã«ãããŠèç©æ§èå
äœã·ãŒã
ã«è»¢åèç©ãããè©Šæäžã®æŸå°æ§æšèç©è³ªã®äœçœ®
æ å ±ãèªã¿åºãããã®èªåºè£ 眮ïŒãããã¯èªåè£
眮ïŒã®äŸã瀺ããã®ã§ããã ïŒïŒèç©æ§èå äœã·ãŒããïŒïŒå èªã¿çšèªåº
éšãïŒïŒæ¬èªã¿çšèªåºéšãïŒïŒã¬ãŒã¶ãŒå æºã
ïŒïŒã¬ãŒã¶ãŒå ãïŒïŒãã€ã«ã¿ãŒãïŒïŒå åå
åšãïŒïŒå¹³é¢åå°é¡ãïŒïŒç§»éæ¹åãïŒïŒïŒå èª
ã¿çšå°å æ§ã·ãŒããïŒïŒïŒå æ€åºåšãïŒïŒïŒå¢å¹
åšãïŒïŒïŒå¶åŸ¡åè·¯ãïŒïŒïŒã¬ãŒã¶ãŒå æºãïŒ
ïŒïŒã¬ãŒã¶ãŒå ãïŒïŒïŒãã€ã«ã¿ãŒãïŒïŒïŒããŒ
ã ã»ãšã¯ã¹ãã³ããŒãïŒïŒïŒå åååšãïŒïŒïŒå¹³
é¢åå°é¡ãïŒïŒïŒfΞã¬ã³ãºãïŒïŒïŒç§»éæ¹åãïŒ
ïŒïŒæ¬èªã¿çšå°å æ§ã·ãŒããïŒïŒïŒå æ€åºåšãïŒ
ïŒïŒå¢å¹ åšãïŒïŒïŒïŒ¡ïŒïŒ€å€æåšãïŒïŒïŒä¿¡å·åŠ
çåè·¯ã
ã«è»¢åèç©ãããè©Šæäžã®æŸå°æ§æšèç©è³ªã®äœçœ®
æ å ±ãèªã¿åºãããã®èªåºè£ 眮ïŒãããã¯èªåè£
眮ïŒã®äŸã瀺ããã®ã§ããã ïŒïŒèç©æ§èå äœã·ãŒããïŒïŒå èªã¿çšèªåº
éšãïŒïŒæ¬èªã¿çšèªåºéšãïŒïŒã¬ãŒã¶ãŒå æºã
ïŒïŒã¬ãŒã¶ãŒå ãïŒïŒãã€ã«ã¿ãŒãïŒïŒå åå
åšãïŒïŒå¹³é¢åå°é¡ãïŒïŒç§»éæ¹åãïŒïŒïŒå èª
ã¿çšå°å æ§ã·ãŒããïŒïŒïŒå æ€åºåšãïŒïŒïŒå¢å¹
åšãïŒïŒïŒå¶åŸ¡åè·¯ãïŒïŒïŒã¬ãŒã¶ãŒå æºãïŒ
ïŒïŒã¬ãŒã¶ãŒå ãïŒïŒïŒãã€ã«ã¿ãŒãïŒïŒïŒããŒ
ã ã»ãšã¯ã¹ãã³ããŒãïŒïŒïŒå åååšãïŒïŒïŒå¹³
é¢åå°é¡ãïŒïŒïŒfΞã¬ã³ãºãïŒïŒïŒç§»éæ¹åãïŒ
ïŒïŒæ¬èªã¿çšå°å æ§ã·ãŒããïŒïŒïŒå æ€åºåšãïŒ
ïŒïŒå¢å¹ åšãïŒïŒïŒïŒ¡ïŒïŒ€å€æåšãïŒïŒïŒä¿¡å·åŠ
çåè·¯ã
Claims (1)
- ãç¹èš±è«æ±ã®ç¯å²ã ïŒ æŸå°æ§æšèãä»ãããçç©äœç±æ¥ã®ç©è³ªãå
é¢å±éããããã®æ¯æäœãããã³èŒå°œæ§èå äœã
çµåå€äžã«åæ£ããŠãªãèå äœå±€ãæããèç©æ§
èå äœã·ãŒããå«ããªãŒãã©ãžãªã°ã©ãã€ãŒçšæž¬
å®ãããã ïŒ åé¢å±éçšã®æ¯æäœãé»æ°æ³³ååé¢çšã®æ¯æ
äœã§ããããšãç¹åŸŽãšããç¹èš±è«æ±ã®ç¯å²ç¬¬ïŒé
èšèŒã®ãªãŒãã©ãžãªã°ã©ãã€ãŒçšæž¬å®ãããã ïŒ åé¢å±éçšã®æ¯æäœãèå±€ã¯ãããã°ã©ãã€
ãŒçšã®æ¯æäœã§ããããšãç¹åŸŽãšããç¹èš±è«æ±ã®
ç¯å²ç¬¬ïŒé èšèŒã®ãªãŒãã©ãžãªã°ã©ãã€ãŒçšæž¬å®
ãããã ïŒ èç©æ§èå äœã·ãŒãããæ¯æäœãšããã®è¡šé¢
ã«èšããããèŒå°œæ§èå äœãšãããåæ£ç¶æ ã§å«
ææ¯æããçµåå€ãšãããªãèå äœå±€ãšãå«ãã
ã®ã§ããããšãç¹åŸŽãšããç¹èš±è«æ±ã®ç¯å²ç¬¬ïŒä¹
è³ïŒé ã®ããããã®é èšèŒã®ãªãŒãã©ãžãªã°ã©ã
ã€ãŒçšæž¬å®ãããã ïŒ èŒå°œæ§èå äœããäºäŸ¡ã®ãŠãŒãããŠã ã«ãã
賊掻ãããŠããã¢ã«ã«ãªåé¡éå±åŒåããã²ã³å
ç©ç³»èå äœã§ããããšãç¹åŸŽãšããç¹èš±è«æ±ã®ç¯
å²ç¬¬ïŒé èšèŒã®ãªãŒãã©ãžãªã°ã©ãã€ãŒçšæž¬å®ã
ããã
Priority Applications (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP57193419A JPS5983058A (ja) | 1982-11-05 | 1982-11-05 | ãªâãã©ãžãªã°ã©ãã€âçšæž¬å®ããã |
| JP58030604A JPS59155759A (ja) | 1982-11-05 | 1983-02-24 | ãªâãã©ãžãªã°ã©ãã€âçšæž¬å®ããã |
| FI834004A FI834004A7 (fi) | 1982-11-05 | 1983-11-01 | Autoradiografisk process |
| DE8383110984T DE3377671D1 (de) | 1982-11-05 | 1983-11-03 | Autoradiographic process |
| EP83110984A EP0111154B1 (en) | 1982-11-05 | 1983-11-03 | Autoradiographic process |
| CA000440451A CA1220564A (en) | 1982-11-05 | 1983-11-04 | Autoradiographic process |
| US07/378,509 US5260190A (en) | 1982-11-05 | 1989-07-06 | Autoradiographic process |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP57193419A JPS5983058A (ja) | 1982-11-05 | 1982-11-05 | ãªâãã©ãžãªã°ã©ãã€âçšæž¬å®ããã |
| JP58030604A JPS59155759A (ja) | 1982-11-05 | 1983-02-24 | ãªâãã©ãžãªã°ã©ãã€âçšæž¬å®ããã |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS5983058A JPS5983058A (ja) | 1984-05-14 |
| JPH0160785B2 true JPH0160785B2 (ja) | 1989-12-25 |
Family
ID=26368992
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP57193419A Granted JPS5983058A (ja) | 1982-11-05 | 1982-11-05 | ãªâãã©ãžãªã°ã©ãã€âçšæž¬å®ããã |
| JP58030604A Granted JPS59155759A (ja) | 1982-11-05 | 1983-02-24 | ãªâãã©ãžãªã°ã©ãã€âçšæž¬å®ããã |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP58030604A Granted JPS59155759A (ja) | 1982-11-05 | 1983-02-24 | ãªâãã©ãžãªã°ã©ãã€âçšæž¬å®ããã |
Country Status (1)
| Country | Link |
|---|---|
| JP (2) | JPS5983058A (ja) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6066858A (en) * | 1992-09-03 | 2000-05-23 | Fuji Photo Film Co., Ltd. | Autoradiographic process |
| CN106796174B (zh) * | 2014-08-29 | 2019-06-14 | å¯å£«éæ ªåŒäŒç€Ÿ | åææ¹æ³ãåæè£ çœ®ååæçšåº |
-
1982
- 1982-11-05 JP JP57193419A patent/JPS5983058A/ja active Granted
-
1983
- 1983-02-24 JP JP58030604A patent/JPS59155759A/ja active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPS5983058A (ja) | 1984-05-14 |
| JPH0456259B2 (ja) | 1992-09-07 |
| JPS59155759A (ja) | 1984-09-04 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JPH0248080B2 (ja) | ||
| US5260190A (en) | Autoradiographic process | |
| JPH0160782B2 (ja) | ||
| US5270162A (en) | Autoradiographic gene-screening method | |
| US4734581A (en) | Autoradiographic process | |
| EP0159523B1 (en) | Autoradiographic process | |
| EP0141382B1 (en) | Method for determining base sequence of dna or dna fragment utilizing autoradiography | |
| EP0115777A2 (en) | Method for determination of base sequence of DNA or DNA fragment | |
| JPH0160784B2 (ja) | ||
| JPH0160785B2 (ja) | ||
| EP0111154B1 (en) | Autoradiographic process | |
| EP0138086B1 (en) | Autoradiographic gene-screening method | |
| JPH0513475B2 (ja) | ||
| JPS59126246A (ja) | ïœïœãããã¯ïœïœïœéšåå解ç©ã®å¡©åºé å決å®æ¹æ³ | |
| JP2945547B2 (ja) | ãªãŒãã©ãžãªã°ã©ã 枬å®æ¹æ³ | |
| JPH0462032B2 (ja) | ||
| JP2945548B2 (ja) | ãªãŒãã©ãžãªã°ã©ã 枬å®æ¹æ³ | |
| JPH0465997B2 (ja) | ||
| JPH06258258A (ja) | ãªãŒãã©ãžãªã°ã©ãã£ãŒç»åã®ãã¬ã¢ã®æ¶å»æ¹æ³ | |
| JPS6118864A (ja) | ïœïœãããã¯ïœïœïœæçç©ã®å¡©åºé å決å®æ¹æ³ | |
| JPH06258448A (ja) | çç©äœã®çµç¹è©Šæã®ç»åå ã®æšèç©è³ªã®äœçœ®ã®åå®æ¹æ³ | |
| JPH0259953B2 (ja) | ||
| JPH0259954B2 (ja) | ||
| JPH1048399A (ja) | èç©æ§èå äœã·ãŒãããã³èç©æ§èå äœã·ãŒããçšããç»åèªã¿åãè£ çœ® |