PT89367A - Melhoramentos na expressao e secrecao de proteinas heterologas em levedura utilizando sequencias guias alfa-factor truncadas - Google Patents

Melhoramentos na expressao e secrecao de proteinas heterologas em levedura utilizando sequencias guias alfa-factor truncadas

Info

Publication number: PT89367A
Authority: PT; Portugal
Prior art keywords: yeast; encodes; precursor polypeptide; precursor; protein
Prior art date: 1987-12-30

Application number

PT89367A

Other languages

English (en)

Other versions

PT89367B (pt

Inventor

Patricia Tekamp-Olson

Original Assignee

Chiron Corp

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1987-12-30

Filing date

1988-12-30

Publication date

1989-12-29

1988-12-30 Application filed by Chiron Corp filed Critical Chiron Corp

1989-12-29 Publication of PT89367A publication Critical patent/PT89367A/pt

1993-09-30 Publication of PT89367B publication Critical patent/PT89367B/pt

Links

240000004808 Saccharomyces cerevisiae Species 0.000 title abstract 5
108090000623 proteins and genes Proteins 0.000 title abstract 3
102000004169 proteins and genes Human genes 0.000 title abstract 2
IXKSXJFAGXLQOQ-XISFHERQSA-N WHWLQLKPGQPMY Chemical compound C([C@@H](C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(C)C)C(=O)N1CCC[C@H]1C(=O)NCC(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(O)=O)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(O)=O)NC(=O)[C@@H](N)CC=1C2=CC=CC=C2NC=1)C1=CNC=N1 IXKSXJFAGXLQOQ-XISFHERQSA-N 0.000 title 1
239000002243 precursor Substances 0.000 abstract 8
229920001184 polypeptide Polymers 0.000 abstract 7
108090000765 processed proteins & peptides Proteins 0.000 abstract 7
102000004196 processed proteins & peptides Human genes 0.000 abstract 7
108010058643 Fungal Proteins Proteins 0.000 abstract 5
NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 abstract 4
108020004414 DNA Proteins 0.000 abstract 3
238000003776 cleavage reaction Methods 0.000 abstract 3
230000013595 glycosylation Effects 0.000 abstract 3
238000006206 glycosylation reaction Methods 0.000 abstract 3
230000007017 scission Effects 0.000 abstract 3
108091026890 Coding region Proteins 0.000 abstract 2
102000004877 Insulin Human genes 0.000 abstract 2
108090001061 Insulin Proteins 0.000 abstract 2
101150045458 KEX2 gene Proteins 0.000 abstract 2
238000001727 in vivo Methods 0.000 abstract 2
229940125396 insulin Drugs 0.000 abstract 2
102000053602 DNA Human genes 0.000 abstract 1
108010016626 Dipeptides Proteins 0.000 abstract 1
102000005593 Endopeptidases Human genes 0.000 abstract 1
108010059378 Endopeptidases Proteins 0.000 abstract 1
101000976075 Homo sapiens Insulin Proteins 0.000 abstract 1
108090000723 Insulin-Like Growth Factor I Proteins 0.000 abstract 1
102100037852 Insulin-like growth factor I Human genes 0.000 abstract 1
125000000729 N-terminal amino-acid group Chemical group 0.000 abstract 1
108010076181 Proinsulin Proteins 0.000 abstract 1
108010076504 Protein Sorting Signals Proteins 0.000 abstract 1
241000235070 Saccharomyces Species 0.000 abstract 1
150000001413 amino acids Chemical class 0.000 abstract 1
PBGKTOXHQIOBKM-FHFVDXKLSA-N insulin (human) Chemical compound C([C@@H](C(=O)N[C@@H](CC(C)C)C(=O)N[C@H]1CSSC[C@H]2C(=O)N[C@H](C(=O)N[C@@H](CO)C(=O)N[C@H](C(=O)N[C@H](C(N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC=3C=CC(O)=CC=3)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CC=3C=CC(O)=CC=3)C(=O)N[C@@H](CSSC[C@H](NC(=O)[C@H](C(C)C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC=3C=CC(O)=CC=3)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](C)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](C(C)C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC=3NC=NC=3)NC(=O)[C@H](CO)NC(=O)CNC1=O)C(=O)NCC(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)NCC(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H]([C@@H](C)O)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H]([C@@H](C)O)C(O)=O)C(=O)N[C@@H](CC(N)=O)C(O)=O)=O)CSSC[C@@H](C(N2)=O)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](C(C)C)NC(=O)[C@@H](NC(=O)CN)[C@@H](C)CC)[C@@H](C)CC)[C@@H](C)O)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@@H](NC(=O)[C@@H](N)CC=1C=CC=CC=1)C(C)C)C1=CN=CN1 PBGKTOXHQIOBKM-FHFVDXKLSA-N 0.000 abstract 1
230000028327 secretion Effects 0.000 abstract 1
230000005026 transcription initiation Effects 0.000 abstract 1
230000005030 transcription termination Effects 0.000 abstract 1
210000005253 yeast cell Anatomy 0.000 abstract 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
- C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/62—Insulins
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/65—Insulin-like growth factors, i.e. somatomedins, e.g. IGF-1, IGF-2
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Genetics & Genomics (AREA)
Biochemistry (AREA)
Biophysics (AREA)
General Health & Medical Sciences (AREA)
Zoology (AREA)
Molecular Biology (AREA)
Mycology (AREA)
Gastroenterology & Hepatology (AREA)
Medicinal Chemistry (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Engineering & Computer Science (AREA)
Diabetes (AREA)
General Engineering & Computer Science (AREA)
Endocrinology (AREA)
Wood Science & Technology (AREA)
Microbiology (AREA)
Biomedical Technology (AREA)
Biotechnology (AREA)
Toxicology (AREA)
Bioinformatics & Cheminformatics (AREA)
Plant Pathology (AREA)
Physics & Mathematics (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Peptides Or Proteins (AREA)
Enzymes And Modification Thereof (AREA)

PT89367A 1987-12-30 1988-12-30 Melhoramentos na expressao e secrecao de proteinas heterologas em levedura utilizando sequencias guias alfa-factor truncadas PT89367B (pt)

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
US13968287A	1987-12-30	1987-12-30

Publications (2)

Publication Number	Publication Date
PT89367A true PT89367A (pt)	1989-12-29
PT89367B PT89367B (pt)	1993-09-30

Family

ID=22487825

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
PT89367A PT89367B (pt)	1987-12-30	1988-12-30	Melhoramentos na expressao e secrecao de proteinas heterologas em levedura utilizando sequencias guias alfa-factor truncadas

Country Status (15)

Country	Link
US (2)	US5602034A (pt)
EP (1)	EP0324274B1 (pt)
JP (1)	JP2793215B2 (pt)
AT (1)	ATE97441T1 (pt)
AU (1)	AU616228B2 (pt)
CA (1)	CA1340772C (pt)
DE (1)	DE3885728T2 (pt)
DK (2)	DK175910B1 (pt)
ES (1)	ES2059547T3 (pt)
FI (1)	FI100995B (pt)
HK (1)	HK139994A (pt)
IE (1)	IE62087B1 (pt)
IL (1)	IL88802A0 (pt)
NO (1)	NO178829C (pt)
PT (1)	PT89367B (pt)

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1988
- 1988-12-22 CA CA000586916A patent/CA1340772C/en not_active Expired - Fee Related
- 1988-12-23 AT AT88312306T patent/ATE97441T1/de not_active IP Right Cessation
- 1988-12-23 ES ES88312306T patent/ES2059547T3/es not_active Expired - Lifetime
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- 1988-12-23 EP EP88312306A patent/EP0324274B1/en not_active Expired - Lifetime
- 1988-12-26 IL IL88802A patent/IL88802A0/xx unknown
- 1988-12-28 NO NO885786A patent/NO178829C/no unknown
- 1988-12-28 FI FI886008A patent/FI100995B/fi not_active IP Right Cessation
- 1988-12-29 DK DK198807285A patent/DK175910B1/da not_active IP Right Cessation
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1994
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1998
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ES2059547T3 (es)	1994-11-16
US5602034A (en)	1997-02-11
DK175910B1 (da)	2005-06-13
FI886008L (fi)	1989-07-01
IE883896L (en)	1989-06-30
DK728588A (da)	1989-07-01
AU616228B2 (en)	1991-10-24
FI100995B (fi)	1998-03-31
IE62087B1 (en)	1994-12-14
DK200500130A (da)	2005-01-27
CA1340772C (en)	1999-09-28
AU2765088A (en)	1989-07-06
NO178829C (no)	1996-06-12
DE3885728T2 (de)	1994-03-10
HK139994A (en)	1994-12-16
DE3885728D1 (de)	1993-12-23
USRE37343E1 (en)	2001-08-28
NO885786D0 (no)	1988-12-28
JPH022339A (ja)	1990-01-08
IL88802A0 (en)	1989-07-31
JP2793215B2 (ja)	1998-09-03
NO178829B (no)	1996-03-04
ATE97441T1 (de)	1993-12-15
EP0324274A1 (en)	1989-07-19
PT89367B (pt)	1993-09-30
DK176075B1 (da)	2006-04-10
EP0324274B1 (en)	1993-11-18
NO885786L (no)	1989-07-03
DK728588D0 (da)	1988-12-29

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