ATE97441T1 - Verbesserung der expression und sekretion heterologener proteine in hefe mittels trunkierter sequenzen des alpha-faktor-leaders. - Google Patents

Verbesserung der expression und sekretion heterologener proteine in hefe mittels trunkierter sequenzen des alpha-faktor-leaders.

Info

Publication number
ATE97441T1
ATE97441T1 AT88312306T AT88312306T ATE97441T1 AT E97441 T1 ATE97441 T1 AT E97441T1 AT 88312306 T AT88312306 T AT 88312306T AT 88312306 T AT88312306 T AT 88312306T AT E97441 T1 ATE97441 T1 AT E97441T1
Authority
AT
Austria
Prior art keywords
yeast
encodes
precursor polypeptide
precursor
protein
Prior art date
Application number
AT88312306T
Other languages
English (en)
Inventor
Patricia Tekamp-Olson
Original Assignee
Chiron Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chiron Corp filed Critical Chiron Corp
Application granted granted Critical
Publication of ATE97441T1 publication Critical patent/ATE97441T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/65—Insulin-like growth factors, i.e. somatomedins, e.g. IGF-1, IGF-2
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Diabetes (AREA)
  • General Engineering & Computer Science (AREA)
  • Endocrinology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Toxicology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
AT88312306T 1987-12-30 1988-12-23 Verbesserung der expression und sekretion heterologener proteine in hefe mittels trunkierter sequenzen des alpha-faktor-leaders. ATE97441T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US13968287A 1987-12-30 1987-12-30

Publications (1)

Publication Number Publication Date
ATE97441T1 true ATE97441T1 (de) 1993-12-15

Family

ID=22487825

Family Applications (1)

Application Number Title Priority Date Filing Date
AT88312306T ATE97441T1 (de) 1987-12-30 1988-12-23 Verbesserung der expression und sekretion heterologener proteine in hefe mittels trunkierter sequenzen des alpha-faktor-leaders.

Country Status (15)

Country Link
US (2) US5602034A (de)
EP (1) EP0324274B1 (de)
JP (1) JP2793215B2 (de)
AT (1) ATE97441T1 (de)
AU (1) AU616228B2 (de)
CA (1) CA1340772C (de)
DE (1) DE3885728T2 (de)
DK (2) DK175910B1 (de)
ES (1) ES2059547T3 (de)
FI (1) FI100995B (de)
HK (1) HK139994A (de)
IE (1) IE62087B1 (de)
IL (1) IL88802A0 (de)
NO (1) NO178829C (de)
PT (1) PT89367B (de)

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Also Published As

Publication number Publication date
NO178829B (no) 1996-03-04
DK728588A (da) 1989-07-01
DK200500130A (da) 2005-01-27
HK139994A (en) 1994-12-16
DE3885728D1 (de) 1993-12-23
PT89367A (pt) 1989-12-29
EP0324274B1 (de) 1993-11-18
AU2765088A (en) 1989-07-06
FI886008L (fi) 1989-07-01
CA1340772C (en) 1999-09-28
EP0324274A1 (de) 1989-07-19
NO885786D0 (no) 1988-12-28
NO885786L (no) 1989-07-03
IE62087B1 (en) 1994-12-14
JPH022339A (ja) 1990-01-08
IL88802A0 (en) 1989-07-31
AU616228B2 (en) 1991-10-24
FI100995B (fi) 1998-03-31
US5602034A (en) 1997-02-11
USRE37343E1 (en) 2001-08-28
PT89367B (pt) 1993-09-30
ES2059547T3 (es) 1994-11-16
DK728588D0 (da) 1988-12-29
NO178829C (no) 1996-06-12
DE3885728T2 (de) 1994-03-10
IE883896L (en) 1989-06-30
DK175910B1 (da) 2005-06-13
JP2793215B2 (ja) 1998-09-03
DK176075B1 (da) 2006-04-10

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