WO1997004103A3 - 5-enol pyruvylshikimate-3-phosphate synthase mutee, gene codant pour cette proteine et plantes transformees contenant ce gene - Google Patents

5-enol pyruvylshikimate-3-phosphate synthase mutee, gene codant pour cette proteine et plantes transformees contenant ce gene Download PDF

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Publication number
WO1997004103A3
WO1997004103A3 PCT/FR1996/001125 FR9601125W WO9704103A3 WO 1997004103 A3 WO1997004103 A3 WO 1997004103A3 FR 9601125 W FR9601125 W FR 9601125W WO 9704103 A3 WO9704103 A3 WO 9704103A3
Authority
WO
WIPO (PCT)
Prior art keywords
gene
mutated
transformed plants
phosphate synthase
protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/FR1996/001125
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English (en)
Other versions
WO1997004103A2 (fr
Inventor
Michel Lebrun
Alain Sailland
Georges Freyssinet
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bayer CropScience SA
Original Assignee
Rhone Poulenc Agrochimie SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=9481324&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=WO1997004103(A3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority to HU9900463A priority Critical patent/HU226089B1/hu
Application filed by Rhone Poulenc Agrochimie SA filed Critical Rhone Poulenc Agrochimie SA
Priority to DE69635995T priority patent/DE69635995T2/de
Priority to JP50636597A priority patent/JP4691733B2/ja
Priority to EP96925812A priority patent/EP0837944B1/fr
Priority to PL96324572A priority patent/PL189453B1/pl
Priority to HU0700738A priority patent/HU226302B1/hu
Priority to SK64-98A priority patent/SK285144B6/sk
Priority to BR9609792A priority patent/BR9609792A/pt
Priority to IL12294196A priority patent/IL122941A0/xx
Priority to AU66191/96A priority patent/AU6619196A/en
Priority to NZ313667A priority patent/NZ313667A/xx
Priority to APAP/P/1998/001195A priority patent/AP886A/en
Priority to SI9630734T priority patent/SI0837944T1/sl
Priority to MX9800562A priority patent/MX9800562A/es
Priority to EA199800138A priority patent/EA199800138A1/ru
Priority to UA98020770A priority patent/UA75315C2/uk
Priority to CA002223875A priority patent/CA2223875C/fr
Priority to RO98-00084A priority patent/RO120849B1/ro
Priority to US08/945,144 priority patent/US6566587B1/en
Publication of WO1997004103A2 publication Critical patent/WO1997004103A2/fr
Publication of WO1997004103A3 publication Critical patent/WO1997004103A3/fr
Anticipated expiration legal-status Critical
Priority to BG102247A priority patent/BG64628B1/bg
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
    • C12N9/1092—3-Phosphoshikimate 1-carboxyvinyltransferase (2.5.1.19), i.e. 5-enolpyruvylshikimate-3-phosphate synthase
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8275—Glyphosate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Botany (AREA)
  • Physiology (AREA)
  • Environmental Sciences (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Peptides Or Proteins (AREA)
  • Catching Or Destruction (AREA)

Abstract

Gène de résistance au glyphosate. 1) Gène muté de résistance au glyphosate. 2) Gène EPSPS comprenant au moins une substitution de la Thréonine 102 par l'isoleucine. 3) Il est utilisable pour l'obtention de plantes transformées résistantes au glyphosate.
PCT/FR1996/001125 1995-07-19 1996-07-18 5-enol pyruvylshikimate-3-phosphate synthase mutee, gene codant pour cette proteine et plantes transformees contenant ce gene Ceased WO1997004103A2 (fr)

Priority Applications (20)

Application Number Priority Date Filing Date Title
US08/945,144 US6566587B1 (en) 1995-07-19 1996-07-18 Mutated 5-enolpyruvylshikimate-3-phosphate synthase, gene coding for said protein and transformed plants containing said gene
APAP/P/1998/001195A AP886A (en) 1995-07-19 1996-07-18 Mutated 5-enol pyruvylshikimate-3-phosphate synthase, gene coding for said protein and transformed plants containing said gene.
DE69635995T DE69635995T2 (de) 1995-07-19 1996-07-18 Mutierte 5-enol-pyruvylshikimat-3-phosphat- synthase, für dieses protein kodierendes gen und dieses gen enthaltende transformierte pflanzen
JP50636597A JP4691733B2 (ja) 1995-07-19 1996-07-18 変異５−エノールピリビルシキミ酸−３−リン酸シンターゼ、この蛋白質をコードする遺伝子及びこの遺伝子を有する形質転換植物
EP96925812A EP0837944B1 (fr) 1995-07-19 1996-07-18 5-enol pyruvylshikimate-3-phosphate synthase mutee, gene codant pour cette proteine et plantes transformees contenant ce gene
PL96324572A PL189453B1 (pl) 1995-07-19 1996-07-18 DNA genu kodującego zmutowaną syntazę 3-enolopiruwoiloszykimiano-5-fosforanową, zmutowane białko EPSPS, gen chimerowy, wektor do transformowania roślin, komórka roślinna, roślina oraz sposoby wytwarzania roślin i ich traktowania
HU0700738A HU226302B1 (en) 1995-07-19 1996-07-18 Transgenic plants containing mutated 5-enol pyruvylshikimate-3-phosphate synthase, and process for producing thereof
SK64-98A SK285144B6 (sk) 1995-07-19 1996-07-18 Sekvencia rastlinnej DNA kódujúca mutovanú 5-enolpyruvylšikimát- 3-fosfátsyntázu, mutovaný rastlinný proteín EPSPS, chimérický gén, vektor, rastlinnábunka, rastlina, spôsob produkcie rastlín a spôsob ošetrenia rastlín
BR9609792A BR9609792A (pt) 1995-07-19 1996-07-18 Gene de dna proteína de epsps mutada gene quimérico vetor para a transformação de plantas célula de planta planta método para a produção de plantas e método de tratamento de plantas
IL12294196A IL122941A0 (en) 1995-07-19 1996-07-18 Mutated 5-enol pyruvylshikimate-3-phosphate synthase gene coding for said protein and transformed plants containing said gene
AU66191/96A AU6619196A (en) 1995-07-19 1996-07-18 Mutated 5-enol pyruvylshikimate-3-phosphate synthase, gene coding for said protein and transformed plants containing said gene
HU9900463A HU226089B1 (en) 1995-07-19 1996-07-18 Mutated 5-enol pyruvylshikimate-3-phosphate synthase, gene coding for said protein and transformed plants containing said gene
MX9800562A MX9800562A (es) 1995-07-19 1996-07-18 5-enol piruvilshikimato-3-fosfato sintasa mutada, gen que codifica para esta proteina, y plantas transformadas que contienen el gen.
NZ313667A NZ313667A (en) 1995-07-19 1996-07-18 a DNA sequence for encoding mutated 5-enol pyruvylshikimate-3-phosphate synthase, the protein and transformed plants containing said DNAne
SI9630734T SI0837944T1 (sl) 1995-07-19 1996-07-18 Mutirana 5-enol pirovilshikimat-3-fosfatna sintaza, gen, ki kodira za ta protein, in transformiranerastline, ki vsebujejo ta gen
EA199800138A EA199800138A1 (ru) 1995-07-19 1996-07-18 Мутантная 5-енолпирувилшикимат-3-фосфатсинтеза, ген, кодирующий этот белок, и трансформированные растения, содержащие этот ген
UA98020770A UA75315C2 (en) 1995-07-19 1996-07-18 A vegetable dna-sequence coding mutant 5-enol-pyruvylshikimate-3-phosphatesyntase (epsps), mutant epsps-protein, transformed plants with an improved tolerance to glyfosate and a method for preparing thereof
CA002223875A CA2223875C (fr) 1995-07-19 1996-07-18 5-enolpyruvylshikimate-3-phosphate synthase mutee, gene codant pour cette proteine et plantes transformees contenant ce gene
RO98-00084A RO120849B1 (ro) 1995-07-19 1996-07-18 5-enolpiruvilshikimat-3-fosfat sintază, genă carecodifică această proteină şi plante care conţin această genă
BG102247A BG64628B1 (bg) 1995-07-19 1998-02-12 Мутантна 5-енолпирувилшикимат-3-фосфат синтетаза, ген, кодиращ този протеин, и трансформирани растения, съдържащи този ген

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR9508979A FR2736926B1 (fr) 1995-07-19 1995-07-19 5-enol pyruvylshikimate-3-phosphate synthase mutee, gene codant pour cette proteine et plantes transformees contenant ce gene
FR95/08979 1995-07-19

Publications (2)

Publication Number Publication Date
WO1997004103A2 WO1997004103A2 (fr) 1997-02-06
WO1997004103A3 true WO1997004103A3 (fr) 1997-03-20

Family

ID=9481324

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/FR1996/001125 Ceased WO1997004103A2 (fr) 1995-07-19 1996-07-18 5-enol pyruvylshikimate-3-phosphate synthase mutee, gene codant pour cette proteine et plantes transformees contenant ce gene

Country Status (31)

Country Link
US (1) US6566587B1 (fr)
EP (2) EP1217073B1 (fr)
JP (2) JP4691733B2 (fr)
KR (1) KR19990029084A (fr)
CN (1) CN1154734C (fr)
AP (1) AP886A (fr)
AT (2) ATE320494T1 (fr)
AU (1) AU6619196A (fr)
BG (1) BG64628B1 (fr)
BR (1) BR9609792A (fr)
CA (1) CA2223875C (fr)
CU (1) CU23172A3 (fr)
CZ (1) CZ295649B6 (fr)
DE (2) DE69635913T2 (fr)
DK (2) DK0837944T3 (fr)
EA (1) EA199800138A1 (fr)
ES (2) ES2256863T3 (fr)
FR (1) FR2736926B1 (fr)
HU (2) HU226089B1 (fr)
IL (1) IL122941A0 (fr)
MX (1) MX9800562A (fr)
NZ (1) NZ313667A (fr)
OA (1) OA10788A (fr)
PL (1) PL189453B1 (fr)
PT (2) PT837944E (fr)
RO (1) RO120849B1 (fr)
SI (2) SI0837944T1 (fr)
SK (1) SK285144B6 (fr)
TR (1) TR199800065T1 (fr)
UA (2) UA80895C2 (fr)
WO (1) WO1997004103A2 (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7527955B2 (en) 2000-10-30 2009-05-05 Pioneer Hi-Bred International, Inc. Glyphosate-N-acetyltransferase (GAT) genes
US7622641B2 (en) 2005-08-24 2009-11-24 Pioneer Hi-Bred Int'l., Inc. Methods and compositions for providing tolerance to multiple herbicides

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US7102055B1 (en) 1997-11-18 2006-09-05 Pioneer Hi-Bred International, Inc. Compositions and methods for the targeted insertion of a nucleotide sequence of interest into the genome of a plant
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US7527955B2 (en) 2000-10-30 2009-05-05 Pioneer Hi-Bred International, Inc. Glyphosate-N-acetyltransferase (GAT) genes
US7531339B2 (en) 2000-10-30 2009-05-12 Verdia, Inc. Glyphosate-N-acetyltransferase (GAT) genes
US7666643B2 (en) 2000-10-30 2010-02-23 Pioneer Hi-Bred International, Inc. Glyphosate-N-acetyltransferase (GAT) genes
US7709702B2 (en) 2000-10-30 2010-05-04 Pioneer Hi-Bred Int'l., Inc. Glyphosate-N-acetyltransferase (GAT) genes
US7714188B2 (en) 2000-10-30 2010-05-11 Pioneer Hi-Bred Int'l., Inc. Glyphosate-N-acetyltransferase (GAT) genes
US7998703B2 (en) 2000-10-30 2011-08-16 Verdia, Inc. Glyphosate-N-acetyltransferase (GAT) genes
US8008547B2 (en) 2000-10-30 2011-08-30 E.I. Dupont De Nemours Glyphosate-N-acetyltransferase (GAT) genes
US8088972B2 (en) 2000-10-30 2012-01-03 Verdia, Inc. Glyphosate-N-acetyltransferase (GAT) genes
US7622641B2 (en) 2005-08-24 2009-11-24 Pioneer Hi-Bred Int'l., Inc. Methods and compositions for providing tolerance to multiple herbicides
US7803992B2 (en) 2005-08-24 2010-09-28 Pioneer Hi-Bred International, Inc. Methods and compositions for expressing an herbicide-tolerant polynucleotide
US7973218B2 (en) 2005-08-24 2011-07-05 Pioneer Hi-Bred International, Inc. Methods and compositions for controlling weeds
US8203033B2 (en) 2005-08-24 2012-06-19 Pioneer Hi-Bred International, Inc. Methods and compositions for providing tolerance to multiple herbicides

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