JPS60173469A - ルミネセンス免疫測定試薬キット及びその為に使用できる化学ルミネセンス標識ハプテン共役体並びにそれらの製造方法 - Google Patents

ルミネセンス免疫測定試薬キット及びその為に使用できる化学ルミネセンス標識ハプテン共役体並びにそれらの製造方法

Info

Publication number: JPS60173469A
Authority: JP; Japan
Prior art keywords: hapten; group; luminescence; conjugate; moles
Prior art date: 1983-07-29
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Pending

Application number

JP59155123A

Other languages

English (en)

Japanese (ja)

Inventor

アンドレ　ガトウ

ダブリー　グラハム　ウード

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Henning Berlin GmbH Chemie- und Pharmawerk

Original Assignee

Henning Berlin GmbH Chemie- und Pharmawerk

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1983-07-29

Filing date

1984-07-25

Publication date

1985-09-06

1984-07-25 Application filed by Henning Berlin GmbH Chemie- und Pharmawerk filed Critical Henning Berlin GmbH Chemie- und Pharmawerk

1985-09-06 Publication of JPS60173469A publication Critical patent/JPS60173469A/ja

Status Pending legal-status Critical Current

Links

239000003153 chemical reaction reagent Substances 0.000 title claims abstract description 23
238000004020 luminiscence type Methods 0.000 title claims description 18
238000004519 manufacturing process Methods 0.000 title claims description 4
IMNFDUFMRHMDMM-UHFFFAOYSA-N N-Heptane Chemical compound CCCCCCC IMNFDUFMRHMDMM-UHFFFAOYSA-N 0.000 title 2
230000036039 immunity Effects 0.000 title 1
108090000765 processed proteins & peptides Proteins 0.000 claims abstract description 4
229920000642 polymer Polymers 0.000 claims description 18
238000003018 immunoassay Methods 0.000 claims description 15
230000027455 binding Effects 0.000 claims description 10
125000005647 linker group Chemical group 0.000 claims description 10
239000007787 solid Substances 0.000 claims description 4
229930186217 Glycolipid Natural products 0.000 claims description 3
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229930195733 hydrocarbon Natural products 0.000 claims description 3
150000002430 hydrocarbons Chemical class 0.000 claims description 3
125000000524 functional group Chemical group 0.000 claims description 2
HVCOBJNICQPDBP-UHFFFAOYSA-N 3-[3-[3,5-dihydroxy-6-methyl-4-(3,4,5-trihydroxy-6-methyloxan-2-yl)oxyoxan-2-yl]oxydecanoyloxy]decanoic acid;hydrate Chemical compound O.OC1C(OC(CC(=O)OC(CCCCCCC)CC(O)=O)CCCCCCC)OC(C)C(O)C1OC1C(O)C(O)C(O)C(C)O1 HVCOBJNICQPDBP-UHFFFAOYSA-N 0.000 claims 2
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VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 10
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102000007562 Serum Albumin Human genes 0.000 description 5
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FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
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HWYHZTIRURJOHG-UHFFFAOYSA-N luminol Chemical compound O=C1NNC(=O)C2=C1C(N)=CC=C2 HWYHZTIRURJOHG-UHFFFAOYSA-N 0.000 description 4
NCAIGTHBQTXTLR-UHFFFAOYSA-N phentermine hydrochloride Chemical compound [Cl-].CC(C)([NH3+])CC1=CC=CC=C1 NCAIGTHBQTXTLR-UHFFFAOYSA-N 0.000 description 4
239000000725 suspension Substances 0.000 description 4
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FALRKNHUBBKYCC-UHFFFAOYSA-N 2-(chloromethyl)pyridine-3-carbonitrile Chemical compound ClCC1=NC=CC=C1C#N FALRKNHUBBKYCC-UHFFFAOYSA-N 0.000 description 2
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RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
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NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
239000004793 Polystyrene Substances 0.000 description 2
CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 2
PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 description 2
QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
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102000004169 proteins and genes Human genes 0.000 description 2
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125000006850 spacer group Chemical group 0.000 description 2
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238000012360 testing method Methods 0.000 description 2
LWIHDJKSTIGBAC-UHFFFAOYSA-K tripotassium phosphate Chemical compound [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 2
VXNZUUAINFGPBY-UHFFFAOYSA-N 1-Butene Chemical compound CCC=C VXNZUUAINFGPBY-UHFFFAOYSA-N 0.000 description 1
DWTFOKXEDHVWPW-UHFFFAOYSA-N 4-(ethyliminomethylideneamino)butan-2-amine;hydrochloride Chemical compound Cl.CCN=C=NCCC(C)N DWTFOKXEDHVWPW-UHFFFAOYSA-N 0.000 description 1
241000143060 Americamysis bahia Species 0.000 description 1
241000193830 Bacillus <bacterium> Species 0.000 description 1
241000894006 Bacteria Species 0.000 description 1
JQUCWIWWWKZNCS-LESHARBVSA-N C(C1=CC=CC=C1)(=O)NC=1SC[C@H]2[C@@](N1)(CO[C@H](C2)C)C=2SC=C(N2)NC(=O)C2=NC=C(C=C2)OC(F)F Chemical compound C(C1=CC=CC=C1)(=O)NC=1SC[C@H]2[C@@](N1)(CO[C@H](C2)C)C=2SC=C(N2)NC(=O)C2=NC=C(C=C2)OC(F)F JQUCWIWWWKZNCS-LESHARBVSA-N 0.000 description 1
OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
XZMCDFZZKTWFGF-UHFFFAOYSA-N Cyanamide Chemical compound NC#N XZMCDFZZKTWFGF-UHFFFAOYSA-N 0.000 description 1
229920002307 Dextran Polymers 0.000 description 1
101000766306 Homo sapiens Serotransferrin Proteins 0.000 description 1
DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
241000699670 Mus sp. Species 0.000 description 1
NQTADLQHYWFPDB-UHFFFAOYSA-N N-Hydroxysuccinimide Chemical compound ON1C(=O)CCC1=O NQTADLQHYWFPDB-UHFFFAOYSA-N 0.000 description 1
IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 description 1
229910019142 PO4 Inorganic materials 0.000 description 1
UGPVGRMTKQLUNS-YFKPBYRVSA-N Phospho lysine Chemical compound NCCCC[C@H](N)C(=O)OP(O)(O)=O UGPVGRMTKQLUNS-YFKPBYRVSA-N 0.000 description 1
OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
102000005686 Serum Globulins Human genes 0.000 description 1
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XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 1
AZFNGPAYDKGCRB-XCPIVNJJSA-M [(1s,2s)-2-amino-1,2-diphenylethyl]-(4-methylphenyl)sulfonylazanide;chlororuthenium(1+);1-methyl-4-propan-2-ylbenzene Chemical compound [Ru+]Cl.CC(C)C1=CC=C(C)C=C1.C1=CC(C)=CC=C1S(=O)(=O)[N-][C@@H](C=1C=CC=CC=1)[C@@H](N)C1=CC=CC=C1 AZFNGPAYDKGCRB-XCPIVNJJSA-M 0.000 description 1
150000003973 alkyl amines Chemical class 0.000 description 1
229910000147 aluminium phosphate Inorganic materials 0.000 description 1
150000001408 amides Chemical class 0.000 description 1
229910021529 ammonia Inorganic materials 0.000 description 1
150000008064 anhydrides Chemical class 0.000 description 1
239000007864 aqueous solution Substances 0.000 description 1
150000004982 aromatic amines Chemical class 0.000 description 1
IAQRGUVFOMOMEM-UHFFFAOYSA-N butene Natural products CC=CC IAQRGUVFOMOMEM-UHFFFAOYSA-N 0.000 description 1
AIXAANGOTKPUOY-UHFFFAOYSA-N carbachol Chemical compound [Cl-].C[N+](C)(C)CCOC(N)=O AIXAANGOTKPUOY-UHFFFAOYSA-N 0.000 description 1
239000004202 carbamide Substances 0.000 description 1
150000001720 carbohydrates Chemical class 0.000 description 1
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239000004020 conductor Substances 0.000 description 1
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GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 1
238000002523 gelfiltration Methods 0.000 description 1
150000004676 glycans Chemical class 0.000 description 1
150000002429 hydrazines Chemical class 0.000 description 1
230000000984 immunochemical effect Effects 0.000 description 1
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230000000977 initiatory effect Effects 0.000 description 1
108010030727 lens intermediate filament proteins Proteins 0.000 description 1
239000007788 liquid Substances 0.000 description 1
125000003588 lysine group Chemical group [H]N([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])(N([H])[H])C(*)=O 0.000 description 1
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KHIWWQKSHDUIBK-UHFFFAOYSA-N periodic acid Chemical class OI(=O)(=O)=O KHIWWQKSHDUIBK-UHFFFAOYSA-N 0.000 description 1
102000013415 peroxidase activity proteins Human genes 0.000 description 1
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239000011574 phosphorus Substances 0.000 description 1
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235000011009 potassium phosphates Nutrition 0.000 description 1
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LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
210000004885 white matter Anatomy 0.000 description 1

Classifications

- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
- G01N33/78—Thyroid gland hormones, e.g. T3, T4, TBH, TBG or their receptors
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
- G01N33/533—Production of labelled immunochemicals with fluorescent label
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/544—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being organic
- G01N33/545—Synthetic resin
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/807—Apparatus included in process claim, e.g. physical support structures
- Y10S436/808—Automated or kit

Landscapes

Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Immunology (AREA)
Engineering & Computer Science (AREA)
Molecular Biology (AREA)
Biomedical Technology (AREA)
Chemical & Material Sciences (AREA)
Hematology (AREA)
Urology & Nephrology (AREA)
Food Science & Technology (AREA)
General Physics & Mathematics (AREA)
Cell Biology (AREA)
Biotechnology (AREA)
Medicinal Chemistry (AREA)
Physics & Mathematics (AREA)
Analytical Chemistry (AREA)
Biochemistry (AREA)
General Health & Medical Sciences (AREA)
Microbiology (AREA)
Pathology (AREA)
Endocrinology (AREA)
Peptides Or Proteins (AREA)
Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Compounds Of Unknown Constitution (AREA)
Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

JP59155123A 1983-07-29 1984-07-25 ルミネセンス免疫測定試薬キット及びその為に使用できる化学ルミネセンス標識ハプテン共役体並びにそれらの製造方法 Pending JPS60173469A (ja)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
DE3327327		1983-07-29
DE3327327.8		1983-07-29

Publications (1)

Publication Number	Publication Date
JPS60173469A true JPS60173469A (ja)	1985-09-06

Family

ID=6205194

Family Applications (2)

Application Number	Title	Priority Date	Filing Date
JP59155123A Pending JPS60173469A (ja)	1983-07-29	1984-07-25	ルミネセンス免疫測定試薬キット及びその為に使用できる化学ルミネセンス標識ハプテン共役体並びにそれらの製造方法
JP59155121A Pending JPS6070359A (ja)	1983-07-29	1984-07-25	有機化学物質や生物学的物質を固定する為の活性化合成樹脂被覆体及びその製造方法

Family Applications After (1)

Application Number	Title	Priority Date	Filing Date
JP59155121A Pending JPS6070359A (ja)	1983-07-29	1984-07-25	有機化学物質や生物学的物質を固定する為の活性化合成樹脂被覆体及びその製造方法

Country Status (5)

Country	Link
US (2)	US4645646A (de)
EP (2)	EP0134307B1 (de)
JP (2)	JPS60173469A (de)
AT (2)	ATE42317T1 (de)
DE (2)	DE3379659D1 (de)

Families Citing this family (48)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
ATE61670T1 (de) *	1985-06-25	1991-03-15	Progen Biotechnik Gmbh	Immuntest zum nachweis von antikoerpern gegen dns.
BE902745A (fr) *	1985-06-26	1985-10-16	Remacle Jose	Methode de dosage par bioluminescence a l'aide d'enzymes immobilisees.
US4794002A (en) *	1985-11-01	1988-12-27	Monsanto Company	Modified polymeric surfaces and process for preparing same
JPS62132172A (ja) *	1985-12-04	1987-06-15	Shionogi & Co Ltd	固相化抗体およびその製造方法
CA1340590C (en) *	1986-07-24	1999-06-08	John C. Voyta	Chemiluminescence enhancement
US4797181A (en) *	1987-08-03	1989-01-10	Gte Laboratories Incorporated	Flavin cofactor modified electrodes and methods of synthesis and use
US5160626A (en) *	1987-09-14	1992-11-03	Gelman Sciences Inc.	Blotting methods using polyaldehyde activated membranes
US4992172A (en) *	1987-09-14	1991-02-12	Gelman Sciences, Inc.	Blotting methods using polyaldehyde activated membranes
US4961852A (en) *	1987-09-14	1990-10-09	Gelman Sciences, Inc.	Polyaldehyde activated membranes
US4824870A (en) *	1987-09-14	1989-04-25	Gelman Sciences, Inc.	Polyaldehyde activated membranes
US6002007A (en) *	1988-02-20	1999-12-14	Dade Behring Marburg Gmbh	Special chemiluminescent acridine derivatives and the use thereof in luminescence immunoassays
DE3844954C2 (de) *	1988-02-20	1998-07-16	Hoechst Ag	Spezielle chemilumineszierende Acridinderivate und ihre Verwendung in Lumineszenzimmunoassays
JPH01280252A (ja) *	1988-05-02	1989-11-10	P C C Technol:Kk	イムノアッセイによる配糖化した低分子化合物の検出方法
US4952519A (en) *	1988-05-02	1990-08-28	E. I. Du Pont De Nemours And Company	Protein immobilization with poly(ethyleneimine) derivatized with a hydroprobic group
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Also Published As

Publication number	Publication date
US4645646A (en)	1987-02-24
EP0135071B1 (de)	1990-05-16
EP0134307B1 (de)	1989-04-19
EP0134307A3 (en)	1986-12-30
US4657873A (en)	1987-04-14
DE3482284D1 (de)	1990-06-21
DE3379659D1 (en)	1989-05-24
ATE52857T1 (de)	1990-06-15
JPS6070359A (ja)	1985-04-22
EP0134307A2 (de)	1985-03-20
EP0135071A2 (de)	1985-03-27
EP0135071A3 (en)	1988-02-03
ATE42317T1 (de)	1989-05-15

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