PL1888636T3 - Sposób oczyszczania przeciwciał - Google Patents

Sposób oczyszczania przeciwciał

Info

Publication number
PL1888636T3
PL1888636T3 PL06753791T PL06753791T PL1888636T3 PL 1888636 T3 PL1888636 T3 PL 1888636T3 PL 06753791 T PL06753791 T PL 06753791T PL 06753791 T PL06753791 T PL 06753791T PL 1888636 T3 PL1888636 T3 PL 1888636T3
Authority
PL
Poland
Prior art keywords
purification
ion exchange
antibodies
immunoglobulin
single step
Prior art date
Application number
PL06753791T
Other languages
English (en)
Other versions
PL1888636T5 (pl
Inventor
Roberto Falkenstein
Burkard Kolb
Maria Smida
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=34936911&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=PL1888636(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Publication of PL1888636T3 publication Critical patent/PL1888636T3/pl
Publication of PL1888636T5 publication Critical patent/PL1888636T5/pl

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B01D15/361—Ion-exchange
    • B01D15/362—Cation-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B01D15/361—Ion-exchange
    • B01D15/363—Anion-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/42—Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
    • B01D15/424—Elution mode
    • B01D15/426—Specific type of solvent
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2866—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against receptors for cytokines, lymphokines, interferons
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/32—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against translation products of oncogenes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/96—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation using ion-exchange
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oncology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
PL06753791.0T 2005-05-25 2006-05-23 Sposób oczyszczania przeciwciał PL1888636T5 (pl)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP05011302 2005-05-25
EP06753791.0A EP1888636B2 (en) 2005-05-25 2006-05-23 Method for the purification of antibodies
PCT/EP2006/004863 WO2006125599A2 (en) 2005-05-25 2006-05-23 Method for the purification of antibodies

Publications (2)

Publication Number Publication Date
PL1888636T3 true PL1888636T3 (pl) 2012-02-29
PL1888636T5 PL1888636T5 (pl) 2023-03-06

Family

ID=34936911

Family Applications (1)

Application Number Title Priority Date Filing Date
PL06753791.0T PL1888636T5 (pl) 2005-05-25 2006-05-23 Sposób oczyszczania przeciwciał

Country Status (18)

Country Link
US (3) US20090098660A1 (pl)
EP (2) EP1888636B2 (pl)
JP (1) JP4852602B2 (pl)
KR (1) KR100967778B1 (pl)
CN (1) CN101180317B (pl)
AT (1) ATE525087T1 (pl)
AU (1) AU2006251307B2 (pl)
BR (1) BRPI0610201B8 (pl)
CA (1) CA2607375C (pl)
DK (1) DK1888636T4 (pl)
ES (2) ES2373402T5 (pl)
FI (1) FI1888636T4 (pl)
IL (2) IL186338A (pl)
MX (1) MX2007014269A (pl)
NO (1) NO345362B1 (pl)
PL (1) PL1888636T5 (pl)
TW (2) TWI391399B (pl)
WO (1) WO2006125599A2 (pl)

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TWI391399B (zh) 2005-05-25 2013-04-01 赫孚孟拉羅股份公司 測定溶離多肽之鹽濃度之方法
RU2009148891A (ru) 2007-06-01 2011-07-20 Ф.Хоффманн-Ля Рош Аг (Ch) Очистка иммуноглобулина
AR067537A1 (es) * 2007-07-17 2009-10-14 Hoffmann La Roche Purificacion de polipeptidos pegilados
CL2008002054A1 (es) * 2007-07-17 2009-05-29 Hoffmann La Roche Metodo para la regeneracion de una columna de cromatografia de intercambio cationico despues de la elusion de eritropoyetina monopeguilada y metodo para obtener una eritropoyetina monopeguilada, incorporando el metodo de regeneracion de la columna de intercambio cationico.
JP5529869B2 (ja) * 2008-08-14 2014-06-25 メルク・シャープ・アンド・ドーム・コーポレーション プロテインａアフィニティークロマトグラフィーを用いる抗体の精製方法
BR112012020964B8 (pt) 2010-03-10 2021-08-24 Hoffmann La Roche método para produzir uma imunoglobulina da subclasse igg1 ou igg4
MX2012011737A (es) * 2010-04-14 2012-11-16 Hoffmann La Roche Remocion de agregado de inmunoglobulina.
BR112013008738B1 (pt) * 2010-10-11 2017-12-19 Abbvie Bahamas Ltd. Method for purification of a protein
WO2012084829A1 (en) 2010-12-21 2012-06-28 F. Hoffmann-La Roche Ag Isoform enriched antibody preparation and method for obtaining it
HRP20180028T4 (hr) * 2011-03-16 2020-10-02 F. Hoffmann - La Roche Ag Kromatografija izmjene iona s poboljšanom selektivnošću za odvajanje polipeptidnih monomera, agregata i fragmenata modulacijom pokretne faze
JP6292718B2 (ja) 2011-07-01 2018-03-14 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 凝集ポリペプチドから単量体ポリペプチドを分離するための方法
US10481164B2 (en) * 2012-03-26 2019-11-19 Amgen Inc. Method for using light scattering in real time to directly monitor and control impurity removal in purification processes
KR20160054597A (ko) * 2013-09-17 2016-05-16 가부시키가이샤 가네카 신규 항체 정제 방법 및 그로부터 얻어지는 항체, 및 양이온 교환기를 사용한 신규 항체 정제법 및 그로부터 얻어지는 항체
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RU2688349C2 (ru) 2014-03-21 2019-05-21 Ф. Хоффманн-Ля Рош Аг In vitro прогнозирование времени полужизни антител in vivo
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BR112018009882A2 (pt) * 2015-11-18 2018-11-13 Merck Patent Gmbh gradientes de ph-sal opostos para separações melhoradas de proteína
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EP3773969A1 (en) * 2018-04-03 2021-02-17 Merck Patent GmbH Cex chromatography media and low salt elution of target proteins from biopharmaceutical feeds
CN111068361B (zh) * 2018-10-22 2022-02-11 中国石油化工股份有限公司 一种己内酰胺离子交换装置及其再生方法
CN110066314B (zh) * 2019-04-01 2023-10-27 上海药明生物技术有限公司 一种高效提高多聚体分离分辨率的亲和纯化工艺
TW202128264A (zh) * 2019-09-25 2021-08-01 瑞士商赫孚孟拉羅股份公司 使用經預測之溶離緩衝鹽濃度的陽離子層析法
AU2021210905A1 (en) * 2020-01-24 2022-09-01 Mural Oncology, Inc. Methods of purification
CN115728433B (zh) * 2021-08-27 2024-11-15 联邦生物科技(珠海横琴)有限公司 IgG4型单克隆抗体电荷异质性的离子交换色谱检测方法
CN116295834B (zh) * 2022-01-05 2026-03-10 上海迈晋生物医药科技有限公司 一种生物制品在层析工艺中的表观稳定性的在线监测方法
CN120939820A (zh) * 2025-07-31 2025-11-14 浙江安各洛生物技术有限公司 一种梯度混合方法及其配套的梯度混合装置

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Also Published As

Publication number Publication date
EP1888636B2 (en) 2022-03-30
TW200716669A (en) 2007-05-01
US20140243508A1 (en) 2014-08-28
ES2389641T5 (es) 2021-06-10
EP1888636B1 (en) 2011-09-21
AU2006251307B2 (en) 2011-08-04
DK1888636T4 (da) 2022-07-18
WO2006125599A3 (en) 2007-06-21
ES2373402T3 (es) 2012-02-03
EP1888636A2 (en) 2008-02-20
CN101180317B (zh) 2013-06-19
CA2607375C (en) 2014-01-21
NO345362B1 (no) 2020-12-28
ES2389641T3 (es) 2012-10-30
CA2607375A1 (en) 2006-11-30
KR100967778B1 (ko) 2010-07-05
US11034754B2 (en) 2021-06-15
TWI391399B (zh) 2013-04-01
BRPI0610201A2 (pt) 2010-06-01
JP2008542218A (ja) 2008-11-27
AU2006251307A1 (en) 2006-11-30
FI1888636T4 (fi) 2023-01-31
WO2006125599A2 (en) 2006-11-30
US9631007B2 (en) 2017-04-25
TWI320788B (en) 2010-02-21
ATE525087T1 (de) 2011-10-15
BRPI0610201B8 (pt) 2021-05-25
IL206993A0 (en) 2012-01-01
KR20080017322A (ko) 2008-02-26
ES2373402T5 (es) 2022-09-14
EP2261252A3 (en) 2011-03-23
IL186338A0 (en) 2008-01-20
US20090098660A1 (en) 2009-04-16
IL186338A (en) 2011-01-31
BRPI0610201B1 (pt) 2020-12-29
CA2607375F (en) 2006-11-30
NO20075091L (no) 2008-01-23
CN101180317A (zh) 2008-05-14
EP2261252B1 (en) 2012-07-25
TW200946543A (en) 2009-11-16
JP4852602B2 (ja) 2012-01-11
EP2261252A2 (en) 2010-12-15
DK1888636T3 (da) 2011-11-14
MX2007014269A (es) 2008-01-24
EP2261252B2 (en) 2020-10-21
PL1888636T5 (pl) 2023-03-06
US20170183394A1 (en) 2017-06-29

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