BRPI0408157B8 - ácido nucléico recombinante, polipeptídeo recombinante com atividade lipase, célula transformada, métodos empregando os mesmos e composição - Google Patents

ácido nucléico recombinante, polipeptídeo recombinante com atividade lipase, célula transformada, métodos empregando os mesmos e composição

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Publication number
BRPI0408157B8
BRPI0408157B8 BRPI0408157A BRPI0408157A BRPI0408157B8 BR PI0408157 B8 BRPI0408157 B8 BR PI0408157B8 BR PI0408157 A BRPI0408157 A BR PI0408157A BR PI0408157 A BRPI0408157 A BR PI0408157A BR PI0408157 B8 BRPI0408157 B8 BR PI0408157B8
Authority
BR
Brazil
Prior art keywords
activity
polypeptides
polynucleotides
employing
hydrolase
Prior art date
Application number
BRPI0408157A
Other languages
English (en)
Inventor
Paul Weiner David
Lyon Jonathan
Wongsakul Sirirung
Hitchman Tim
T Bornscheuer Uwe
Original Assignee
Dsm Food Specialties B V
Dsm Ip Assets Bv
Diversa Corp
Verenium Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dsm Food Specialties B V, Dsm Ip Assets Bv, Diversa Corp, Verenium Corp filed Critical Dsm Food Specialties B V
Publication of BRPI0408157A publication Critical patent/BRPI0408157A/pt
Publication of BRPI0408157B1 publication Critical patent/BRPI0408157B1/pt
Publication of BRPI0408157B8 publication Critical patent/BRPI0408157B8/pt

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    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
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    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
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Abstract

hidrolases, ácidos nucléicos e métodos empregando. a invenção refere-se a hidrolases, polinucleotídeos e métodos de empregar estes polinucleotídeos e polipeptídeos. em um aspecto, a invenção é direcionada a polipeptídeos, por exemplo, enzimas, tendo uma atividade de hidrolase, por exemplo, uma esterase, acilase, lipase, fosfolipase (por exemplo, atividade de fosfolipase a, b, c e d, atividade de patatina, atividade de hidrolase de acila de lipídeo (lah)) ou atividade de protease, incluindo atividade de hidrolase termotolerante e termoestável, e polinucleotídeos codificando estas enzimas, e preparar e empregar estes polinucleotídeos e polipeptídeos. as atividades de hidrolase dos polipeptídeos e peptídeos da invenção incluem atividade de esterase, atividade de lipase (hidrólise de lipídeos), reações de acidólise (para substituir um ácido graxo esterificado com um ácido graxo livre), reações de transesterificação (permuta de ácidos graxos entre triglicerídeos), síntese de éster, reações de intercâmbio de éster, atividade de fosfolipase e atividade de protease (hidrólise de ligações de peptídeo). os polipeptídeos da invenção podem ser empregados em uma variedade de contextos industriais, agrícolas e farmacêuticos, incluindo a fabricação de cosméticos e nutracêuticos. em outro aspecto, os polipeptídeos da invenção são empregados para sintetizarem produtos quirais enantiomericamente puros.
BRPI0408157A 2003-03-07 2004-03-08 ácido nucléico recombinante, polipeptídeo recombinante com atividade lipase, célula transformada, métodos empregando os mesmos e composição BRPI0408157B8 (pt)

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