PL191529B1 - Nowa odmiana proteazy, cząsteczka DNA kodująca nową odmianę proteazy, wektor zawierający tę cząsteczkę oraz karma dla zwierząt i kompozycje zawierające nową odmianę proteazy - Google Patents
Nowa odmiana proteazy, cząsteczka DNA kodująca nową odmianę proteazy, wektor zawierający tę cząsteczkę oraz karma dla zwierząt i kompozycje zawierające nową odmianę proteazyInfo
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- PL191529B1 PL191529B1 PL340600A PL34060098A PL191529B1 PL 191529 B1 PL191529 B1 PL 191529B1 PL 340600 A PL340600 A PL 340600A PL 34060098 A PL34060098 A PL 34060098A PL 191529 B1 PL191529 B1 PL 191529B1
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- positions
- substitution
- amino acid
- subtilisin
- residue
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Abstract
1. Odmiana proteazy, znamienna tym, ze zawiera podstawienie aminowasowe w pozycji reszty odpowiadajacej pozycji 103 oraz przynajmniej jednej z pozycji 236 i 245 w subtylizynie z Bacillus amyloliquefaciens oraz podstawienie przynajmniej jednego aminokwasu w pozycji reszty odpowiadajacej nastepujacym pozycjom w tej subtylizynie: 1, 3, 4, 8, 10, 12, 13, 16, 17, 18, 19, 20, 21, 22, 24, 27, 33, 37, 38, 42, 43, 48, 55, 57, 58, 61, 62, 68, 72, 75, 76, 77, 78, 79, 86, 87, 89, 97, 98, 99, 101, 102, 104, 106, 107, 109, 111, 114, 116, 117, 119, 121, 123, 126, 128, 130, 131, 133, 134, 137, 140, 141, 142, 146, 147, 158, 159, 160, 166, 167, 170, 173, 174, 177,181, 182, 183, 184, 185, 188, 192, 194, 198, 203, 204, 205, 206, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 222, 224, 227, 228, 230, 232, 237, 238, 240, 242, 243, 244, 245, 246, 247, 248, 249, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 265, 268, 269, 270, 271, 272, 274 oraz 275, przy czym jesli odmiana obejmuje podstawienie jednoczesnie w pozycjach 103 i 76, to wystepuje równiez pod- stawienie w przynajmniej jednej z pozycji innych niz odpowiadajace nastepujacym pozycjom 27, 99, 101, 104, 107, 109, 123, 128, 166, 204, 206, 210, 216, 217, 218, 222, 260, 265 lub 274. 9. Wektor ekspresyjny, znamienny tym, ze obejmuje sekwencje regulatorowe funkcjonalnie polaczone z sekwencja DNA kodujaca odmiane proteazy, która zawiera podstawienie aminowasowe w pozycji reszty odpowiadajacej pozycji 103 oraz przynajmniej jednej z pozycji 236 i 245 w subtylizynie z Bacillus amyloliquefaciens o sekwencji nr 1 oraz podstawienie przynajmniej jednego aminokwasu w pozycji reszty odpowiadajacej nastepujacym pozycjom w tej subtylizynie: 1, 3, 4, 8, 10, 12, 13, 16, 17, 18, 19, 20, 21, 22, 24, 27, 33, 37, 38, 42, 43, 48, 55, 57, 58, 61, 62, 68, 72, 75, 76, 77, 78, 79, 86, 87, 89, 97, 98, 99, 101, 102, 104, 106, 107, 109, 111, 114, 116, 117, 119, 121, 123, 126, 128, 130, 131, 133, 134, 137, 140, 141, 142, 146, 147, 158, 159, 160, 166, 167, 170, 173, 174, 177,181, 182, 183, 184, 185, 188, 192, 194, 198, 203, 204, 205, 206, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 222, 224, 227, 228, 230, 232, 237, 238, 240, 242, 243, 244, 245, 246, 247, 248, 249, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 265, 268, 269, 270, 271, 272, 274 oraz 275, przy czym jesli odmiana obejmuje podstawienie jednoczesnie w pozycjach 103 i 76, to wystepuje równiez pod- stawienie w przynajmniej jednej z pozycji innych niz odpowiadajace nastepujacym pozycjom 27, 99, 101, 104, 107, 109, 123, 128, 166, 204, 206, 210, 216, 217, 218, 222, 260, 265 lub 274, korzystnie sekwencja regulatorowa obejmuje promotor, sekwencje operatora, sekwencje kodujaca miejsce wiazania rybosomu z mRNA oraz sekwencje regulujace terminacje transkrypcji i translacji. PL PL PL PL PL PL PL
Description
Przedmiotem wynalazku jest nowa odmiana proteazy, cząsteczka DNA kodująca nową odmianę proteazy, wektor ekspresyjny obejmujący DNA kodujący nową odmianę proteazy i komórka gospodarza zawierająca wektor ekspresyjny obejmujący DNA kodujący nową odmianę proteazy oraz karma dla zwierząt i kompozycja do czyszczenia i kompozycja do obróbki tekstyliów zawierające nową odmianę proteazy.
Proteazy serynowe stanowią podgrupę hydrolaz karbonylowych. Obejmują one klasę różnorodnych enzymów o szerokim zakresie specyficzności i funkcji biologicznych, Stroud. R. Sci. Amer., 131: 74-88. Pomimo ich funkcjonalnej różnorodności, katalityczną aktywność proteaz serynowych uzyskano dla przynajmniej dwóch genetycznie odmiennych rodzin enzymów:
1) subtylizyn oraz
2) pokrewnych pochodzącej od ssaków chymotrypsynie i homologicznych proteaz bakteryjnych (np. trypsyna i trypsyna z S. gresius).
Te dwie rodziny proteaz serynowych wykazują niezwykle podobne mechanizmy katalizy, Kraut. J. (1977), Annu. Rev. Biochem., 46: 331-358. Ponadto, chociaż ich zasadnicza budowa nie jest zbliżona, struktura trzeciorzędowa obejmuje zachowaną katalityczną triadę aminokwasową złożoną z seryny, histydyny i asparaginianu.
Subtylizyny są proteazami serynowymi (przybliżona masa cząsteczkowa 27500), wydzielanymi w dużych ilościach przez bakterie z rodzaju Bacillus i inne bakterie. Białkową sekwencję subtylizyny określono dla przynajmniej dziewięciu różnych gatunków Bacillus, Markland F. S. i in. (1983), Hoppe-Seyler's Z. Physiol. Chem., 364: 1537-1540.
Przedstawiono trójwymiarową strukturę krystalograficzną subtylizyn z Bacillus amyloliquefaciens, Bacillus licheniformis oraz kilku naturalnych odmian B. lentus. Te badania wykazały, że chociaż subtylizyna nie jest genetycznie spokrewniona z ssaczymi proteazami serynowymi, to ma podobną strukturę miejsc aktywnych.
Rentgenowskie badania struktury krystalicznej subtylizyny zawierającej kowalencyjnie związane inhibitory peptydowe (Robertus J. D., i in. (1972), Biochemistry. 11: 2439-2449) lub kompleksy (Robertus J. D., i in.(1976), J. Biol. Chem., 251: 1097-1103) również dostarczyły informacji o aktywnych pozycjach i przypuszczalnej lokalizacji wiązania substratów subtylizyny.
Ponadto, wykonano wiele badań kinetycznych i chemicznych odmian subtylizyny, jak opisano w pracach Svendsen, B. (1976), Carlsberg. Res. Commun., 41: 237-291; Markland F. S., tamże).
Istnieje przynajmniej jedno doniesienie o eksperymencie, w którym boczny łańcuch metioniny w pozycji reszty 222 subtylizyny poddano konwersji działaniem nadtlenku wodoru, uzyskując sulfotlenek metioniny (Stauffer, D. C., i in. (1965) J. Biol. Chem. 244: 5333-5338).
Przeprowadzono także oraz obszerne badania techniką mutagenezy punktowej (Wells i Estell (1988) TIBS 13: 291-297).
Przedmiotem wynalazku jest nowa odmiana proteazy, która zawiera podstawienie aminowasowe w pozycji reszty odpowiadającej pozycji 103 oraz przynajmniej jednej z pozycji 236 i 245 w subtylizynie z Bacillus amyloliquefaciens oraz podstawienie przynajmniej jednego aminokwasu w pozycji reszty odpowiadającej następującym pozycjom w tej subtylizynie: 1, 3, 4, 8, 10, 12,13, 16, 17, 18, 19, 20, 21, 22, 24, 27, 33, 37, 38, 42, 43, 48, 55, 57, 58, 61, 62, 68, 72, 75, 76, 77, 78, 79, 86, 87, 89, 97, 98, 99, 101, 102, 104, 106, 107, 109, 111, 114, 116, 117, 119, 121, 123, 126, 128, 130, 131, 133, 134, 137, 140, 141, 142, 146, 147, 158, 159, 160, 166, 167, 170, 173, 174, 177,181, 182, 183, 184, 185, 188, 192, 194, 198, 203, 204, 205, 206, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 222, 224, 227, 228, 230, 232, 237, 238, 240, 242, 243, 244, 245, 246, 247, 248, 249, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 265, 268, 269, 270, 271, 272, 274 oraz 275, przy czym jeśli odmiana obejmuje podstawienie jednocześnie w pozycjach 103 i 76, to występuje również podstawienie w przynajmniej jednej z pozycji innych niż odpowiadające następującym pozycjom 27, 99, 101, 104, 107, 109, 123, 128, 166, 204, 206, 210, 216, 217, 218, 222, 260, 265 lub 274.
Korzystnie odmiana proteazy zawiera podstawienie aminowasowe w pozycji reszty odpowiadającej pozycji 103 i 236 oraz w przynajmniej jednej z pozycji: 1, 9, 12, 61, 62, 68, 76, 97, 98, 97, 98, 101, 102, 104, 109, 130, 131, 159, 183, 185, 205, 209, 210, 211, 212, 213, 215, 217, 230, 232, 248, 252, 257, 260, 270 i 275 albo podstawienie aminowasowe w pozycji reszty odpowiadającej pozycji 103 i 245 oraz w przynajmniej jednej z pozycji: 1, 9, 12, 61, 62, 68, 76, 97, 98, 97, 98, 101, 102, 104,
PL 191 529 B1
109, 130, 131, 159, 183, 185, 205, 209, 210, 211, 212, 213, 215, 217, 230, 232, 248, 252, 257, 260, 270 i 275.
W innym korzystnym rozwiązaniu odmiana proteazy według zawiera podstawienie aminowasowe w pozycji reszty odpowiadającej pozycji 103, 236 i 245 oraz w przynajmniej jednej z pozycji: 1, 9, 12, 61, 62, 68, 76, 97, 98, 97, 98, 101, 102, 104, 109, 130, 131, 159, 183, 185, 205, 209, 210, 211, 212, 213, 215, 217, 230, 232, 248, 252, 257, 260, 270 i 275.
Korzystnie odmiana proteazy zawiera podstawienie aminowasowe w pozycji reszty odpowiadającej pozycji 159.
W korzystnym rozwiązaniu odmiana proteazy pochodzi od subtylizyny z Bacillus, korzystniej od subtylizyny z Bacillus lentus.
Inną odmianą wynalazku jest cząsteczka DNA, która zawiera kodony kodujące odmianę proteazy, która zawiera podstawienie aminowasowe w pozycji reszty odpowiadającej pozycji 103 oraz przynajmniej jednej z pozycji 236 i 245 w subtylizynie z Bacillus amyloliquefaciens o sekwencji nr 1 oraz podstawienie przynajmniej jednego aminokwasu w pozycji reszty odpowiadającej następującym pozycjom wtej subtylizynie: 1, 3, 4, 8, 10, 12, 13, 16, 17, 18, 19, 20, 21, 22, 24, 27, 33, 37, 38, 42, 43, 48, 55, 57, 58, 61, 62, 68, 72, 75, 76, 77, 78, 79, 86, 87, 89, 97, 98, 99, 101, 102, 104, 106, 107, 109, 111, 114, 116, 117, 119, 121, 123, 126, 128, 130, 131, 133, 134, 137, 140, 141, 142, 146, 147, 158, 159, 160, 166, 167, 170, 173, 174, 177,181, 182, 183, 184, 185, 188, 192, 194, 198, 203, 204, 205, 206, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 222, 224, 227, 228, 230, 232, 237, 238, 240, 242, 243, 244, 245, 246, 247, 248, 249, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 265, 268, 269, 270, 271, 272, 274 oraz 275, przy czym jeśli odmiana obejmuje podstawienie jednocześnie w pozycjach 103 i 76, to występuje również podstawienie w przynajmniej jednej z pozycji innych niż odpowiadające następującym pozycjom 27, 99, 101, 104, 107, 109, 123, 128, 166, 204, 206, 210, 216, 217, 218, 222, 260, 265 lub 274.
Kolejną odmianą wynalazku jest wektor ekspresyjny, który obejmuje sekwencje regulatorowe funcjonalnie połączone z sekwencją DNA kodującą odmianę proteazy, która zawiera podstawienie aminowasowe w pozycji reszty odpowiadającej pozycji 103 oraz przynajmniej jednej z pozycji 236 i245 w subtylizynie z Bacillus amyloliquefaciens o sekwencji nr 1 oraz podstawienie przynajmniej jednego aminokwasu w pozycji reszty odpowiadającej następującym pozycjom wtej subtylizynie: 1, 3, 4, 8, 10, 12, 13, 16, 17, 18, 19, 20, 21, 22, 24, 27, 33, 37, 38, 42, 43, 48, 55, 57, 58, 61, 62, 68, 72, 75, 76, 77, 78, 79, 86, 87, 89, 97, 98, 99, 101, 102, 104, 106, 107, 109, 111, 114, 116, 117, 119, 121, 123, 126, 128, 130, 131, 133, 134, 137, 140, 141, 142, 146, 147, 158, 159, 160, 166, 167, 170, 173, 174, 177,181, 182, 183, 184, 185, 188, 192, 194, 198, 203, 204, 205, 206, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 222, 224, 227, 228, 230, 232, 237, 238, 240, 242, 243, 244, 245, 246, 247, 248, 249, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 265, 268, 269, 270, 271, 272, 274 oraz 275, przy czym jeśli odmiana obejmuje podstawienie jednocześnie w pozycjach 103 i 76, to występuje również podstawienie w przynajmniej jednej z pozycji innych niż odpowiadające następującym pozycjom 27, 99, 101, 104, 107, 109, 123, 128, 166, 204, 206, 210, 216, 217, 218, 222, 260, 265 lub 274, korzystnie sekwencja regulatorowa obejmuje promotor, sekwencje operatora, sekwencję kodującą miejsce wiązania rybosomu z mRNA oraz sekwencje regulujące terminację transkrypcji i translacji.
Kolejną odmianą wynalazku jest komórka gospodarza znamienna tym, że jest transforomowana wektorem ekspresyjnym obejmującym sekwencje regulatorowe funcjonalnie połączone z sekwencją DNA kodującą odmianę proteazy, która zawiera podstawienie aminowasowe w pozycji reszty odpowiadającej pozycji 103 oraz przynajmniej jednej z pozycji 236 i 245 w subtylizynie z Bacillus amyloliquefaciens o sekwencji nr 1 oraz podstawienie przynajmniej jednego aminokwasu w pozycji reszty odpowiadającej następującym pozycjom w tej subtylizynie: 1, 3, 4, 8, 10, 12, 13, 16, 17, 18, 19, 20, 21, 22, 24, 27, 33, 37, 38, 42, 43, 48, 55, 57, 58, 61, 62, 68, 72, 75, 76, 77, 78, 79, 86, 87, 89, 97, 98, 99, 101, 102, 104, 106, 107, 109, 111, 114, 116, 117, 119, 121, 123, 126, 128, 130, 131, 133, 134, 137, 140, 141, 142, 146, 147, 158, 159, 160, 166, 167, 170, 173, 174, 177,181, 182, 183, 184, 185, 188, 192, 194, 198, 203, 204, 205, 206, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 222, 224, 227, 228, 230, 232, 237, 238, 240, 242, 243, 244, 245, 246, 247, 248, 249, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 265, 268, 269, 270, 271, 272, 274 oraz 275, przy czym jeśli odmiana obejmuje podstawienie jednocześnie w pozycjach 103 i 76, to występuje również podstawienie w przynajmniej jednej z pozycji innych niż odpowiadające następującym pozycjom 27, 99, 101, 104,
PL 191 529 B1
107, 109, 123, 128, 166, 204, 206, 210, 216, 217, 218, 222, 260, 265 lub 274, korzystnie szczep Bacillus BG2036, Bacillus subtilis 1168 oraz B. licheniformis oraz B. lentus.
Przedmiotem wynalazku jest ponadto kompozycja do czyszczenia, która jako środek aktywny zawiera odmianę proteazy, która zawiera podstawienie aminowasowe w pozycji reszty odpowiadającej pozycji 103 oraz przynajmniej jednej z pozycji 236 i 245 w subtylizynie z Bacillus amyloliquefaciens o sekwencji nr 1 oraz podstawienie przynajmniej jednego aminokwasu w pozycji reszty odpowiadającej następującym pozycjom w tej subtylizynie: 1, 3, 4, 8, 10, 12, 13, 16, 17, 18, 19, 20, 21, 22, 24, 27, 33, 37, 38, 42, 43, 48, 55, 57, 58, 61, 62, 68, 72, 75, 76, 77, 78, 79, 86, 87, 89, 97, 98, 99, 101, 102, 104, 106, 107, 109, 111, 114, 116, 117, 119, 121, 123, 126, 128, 130, 131, 133, 134, 137, 140, 141, 142, 146, 147, 158, 159, 160, 166, 167, 170, 173, 174, 177,181, 182, 183, 184, 185, 188, 192, 194, 198, 203, 204, 205, 206, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 222, 224, 227, 228, 230, 232, 237, 238, 240, 242, 243, 244, 245, 246, 247, 248, 249, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 265, 268, 269, 270, 271, 272, 274 oraz 275, przy czym jeśli odmiana obejmuje podstawienie jednocześnie w pozycjach 103 i 76, to występuje również podstawienie w przynajmniej jednej z pozycji innych niż odpowiadające następującym pozycjom 27, 99, 101, 104, 107, 109, 123, 128, 166, 204, 206, 210, 216, 217, 218, 222, 260, 265 lub 274, korzystnie w ilości od 0,01% do 5% wagowych w proszkowych lub ciekłych środkach czyszczących.
Innym przedmiotem wynalazku jest karma dla zwierząt, która zawiera odmianę proteazy, która zawiera podstawienie aminowasowe w pozycji reszty odpowiadającej pozycji 103 oraz przynajmniej jednej z pozycji 236 i 245 w subtylizynie z Bacillus amyloliquefaciens o sekwencji nr 1 oraz podstawienie przynajmniej jednego aminokwasu w pozycji reszty odpowiadającej następującym pozycjom wtej subtylizynie: 1, 3, 4, 8, 10, 12, 13, 16, 17, 18, 19, 20, 21, 22, 24, 27, 33, 37, 38, 42, 43, 48, 55, 57, 58, 61, 62, 68, 72, 75, 76, 77, 78, 79, 86, 87, 89, 97, 98, 99, 101, 102, 104, 106, 107, 109, 111, 114, 116, 117, 119, 121, 123, 126, 128, 130, 131, 133, 134, 137, 140, 141, 142, 146, 147, 158, 159, 160, 166, 167, 170, 173, 174, 177,181, 182, 183, 184, 185, 188, 192, 194, 198, 203, 204, 205, 206, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 222, 224, 227, 228, 230, 232, 237, 238, 240, 242, 243, 244, 245, 246, 247, 248, 249, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 265, 268, 269, 270, 271, 272, 274 oraz 275, przy czym jeśli odmiana obejmuje podstawienie jednocześnie w pozycjach 103 i 76, to występuje również podstawienie w przynajmniej jednej z pozycji innych niż odpowiadające następującym pozycjom 27, 99, 101, 104, 107, 109, 123, 128, 166, 204, 206, 210, 216, 217, 218, 222, 260, 265 lub 274, korzystnie w ilości od 0,01% do 5% wagowych.
Inną odmianą wynalazku jest kompozycja do obróbki tekstyliów, która zawiera odmianę proteazy, która zawiera podstawienie aminowasowe w pozycji reszty odpowiadającej pozycji 103 oraz przynajmniej jednej z pozycji 236 i 245 w subtylizynie z Bacillus amyloliquefaciens o sekwencji nr 1 oraz podstawienie przynajmniej jednego aminokwasu w pozycji reszty odpowiadającej następującym pozycjom w tej subtylizynie: 1, 3, 4, 8, 10, 12, 13, 16, 17, 18, 19, 20, 21, 22, 24, 27, 33, 37, 38, 42, 43, 48, 55, 57, 58, 61, 62, 68, 72, 75, 76, 77, 78, 79, 86, 87, 89, 97, 98, 99, 101, 102, 104, 106, 107, 109, 111, 114, 116, 117, 119, 121, 123, 126, 128, 130, 131, 133, 134, 137, 140, 141, 142, 146, 147, 158, 159, 160, 166, 167, 170, 173, 174, 177,181, 182, 183, 184, 185, 188, 192, 194, 198, 203, 204, 205, 206, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 222, 224, 227, 228, 230, 232, 237, 238, 240, 242, 243, 244, 245, 246, 247, 248, 249, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 265, 268, 269, 270, 271, 272, 274 oraz 275, przy czym jeśli odmiana obejmuje podstawienie jednocześnie w pozycjach 103 i 76, to występuje również podstawienie w przynajmniej jednej z pozycji innych niż odpowiadające następującym pozycjom 27, 99, 101, 104, 107, 109, 123, 128, 166, 204, 206, 210, 216, 217, 218, 222, 260, 265 lub 274, korzystnie w ilości od 0,01% do 5% wagowych.
Bardziej korzystne odmiany proteaz obejmują zestaw podstawień wybrany z grupy zawierającej pozycje reszt odpowiadające pozycjom w subtylizynie z Bacillus amyloliquefaciens, podanym w tabeli 1.
PL 191 529 B1
Tabel a 1
| 76 | 103 | 104 | 222 | |||||||||
| 76 | 98 | 103 | 104 | |||||||||
| 76 | 78 | 103 | 104 | |||||||||
| 76 | 103 | 104 | 107 | |||||||||
| 4 | 76 | 103 | 104 | |||||||||
| 76 | 103 | 104 | 246 | |||||||||
| 76 | 77 | 103 | 104 | |||||||||
| 76 | 103 | 104 | 183 | 218 | ||||||||
| 16 | 76 | 103 | 104 | 248 | ||||||||
| 1 | 76 | 103 | 104 | |||||||||
| 76 | 103 | 104 | 261 | |||||||||
| 76 | 103 | 104 | 160 | |||||||||
| 76 | 103 | 104 | 216 | |||||||||
| 17 | 76 | 103 | 104 | |||||||||
| 37 | 76 | 103 | 104 | |||||||||
| 76 | 77 | 103 | 104 | 174 | ||||||||
| 38 | 76 | 103 | 104 | |||||||||
| 38 | 76 | 103 | 104 | 237 | ||||||||
| 8 | 76 | 103 | 104 | |||||||||
| 76 | 103 | 104 | 183 | |||||||||
| 19 | 76 | 103 | 104 | |||||||||
| 13 | 76 | 103 | 104 | |||||||||
| 19 | 76 | 103 | 104 | |||||||||
| 76 | 103 | 104 | 184 | |||||||||
| 76 | 103 | 104 | 252 | |||||||||
| 76 | 103 | 104 | 259 | |||||||||
| 76 | 103 | 104 | 251 | |||||||||
| 76 | 86 | 103 | 104 | |||||||||
| 72 | 76 | 103 | 104 | 185 | ||||||||
| 76 | 103 | 104 | 237 | 274 | ||||||||
| 76 | 103 | 104 | 160 | |||||||||
| 76 | 103 | 104 | 228 | |||||||||
| 55 | 76 | 103 | 104 | 240 | ||||||||
| 76 | 103 | 104 | 254 | |||||||||
| 76 | 103 | 104 | 204 | |||||||||
| 76 | 103 | 104 | 204 | |||||||||
| 43 | 76 | 103 | 104 |
PL 191 529 B1 ciąg dalszy tabeli 1
| 76 | 103 | 104 | 159 | |||||||||
| 10 | 76 | 103 | 104 | 177 | ||||||||
| 58 | 76 | 103 | 104 | |||||||||
| 76 | 103 | 104 | 270 | |||||||||
| 76 | 103 | 104 | 185 | |||||||||
| 27 | 76 | 103 | 104 | |||||||||
| 76 | 103 | 104 | 262 | |||||||||
| 76 | 78 | 103 | 104 | |||||||||
| 24 | 76 | 103 | 104 | |||||||||
| 76 | 103 | 104 | 166 | 236 | 251 | |||||||
| 17 | 76 | 103 | 104 | 237 | ||||||||
| 76 | 103 | 104 | 130 | |||||||||
| 76 | 103 | 104 | 109 | |||||||||
| 76 | 99 | 103 | 104 | 204 | ||||||||
| 76 | 103 | 104 | 181 | |||||||||
| 12 | 76 | 103 | 104 | |||||||||
| 76 | 103 | 104 | 212 | 271 | ||||||||
| 76 | 103 | 104 | 252 | 261 | ||||||||
| 76 | 103 | 104 | 242 | |||||||||
| 76 | 103 | 104 | 271 | |||||||||
| 12 | 76 | 103 | 104 | 242 | ||||||||
| 43 | 76 | 103 | 104 | 116 | 183 | |||||||
| 76 | 103 | 104 | 258 | |||||||||
| 76 | 103 | 104 | 271 | |||||||||
| 61 | 76 | 103 | 104 | |||||||||
| 38 | 76 | 103 | 104 | 182 | 263 | |||||||
| 76 | 103 | 104 | 182 | 272 | ||||||||
| 76 | 103 | 104 | 109 | 246 | ||||||||
| 76 | 87 | 103 | 104 | 206 | 249 | 265 | ||||||
| 76 | 103 | 104 | 137 | 238 | 271 | |||||||
| 103 | 104 | 228 | ||||||||||
| 76 | 103 | 104 | 182 | 198 | ||||||||
| 21 | 76 | 103 | 104 | 182 | ||||||||
| 76 | 103 | 104 | 119 | 137 | ||||||||
| 76 | 103 | 104 | 137 | 248 | ||||||||
| 13 | 76 | 103 | 104 | 206 | ||||||||
| 76 | 103 | 104 | 206 |
PL 191 529 B1 ciąg dalszy tabeli 1
| 76 | 103 | 104 | 212 | 258 | ||||||||
| 58 | 76 | 103 | 104 | 271 | ||||||||
| 76 | 103 | 104 | 206 | 261 | ||||||||
| 4 | 76 | 103 | 104 | 206 | ||||||||
| 76 | 77 | 103 | 104 | 206 | ||||||||
| 76 | 103 | 104 | 158 | |||||||||
| 76 | 103 | 104 | 206 | |||||||||
| 4 | 76 | 103 | 104 | 159 | 217 | 251 | ||||||
| 4 | 76 | 103 | 104 | 159 | 217 | 252 | ||||||
| 76 | 77 | 103 | 104 | 133 | 185 | 251 | ||||||
| 76 | 103 | 104 | 159 | 206 | 244 | |||||||
| 4 | 76 | 103 | 104 | 188 | ||||||||
| 4 | 76 | 103 | 104 | 158 | ||||||||
| 76 | 77 | 103 | 104 | 185 | ||||||||
| 76 | 103 | 104 | 206 | 251 | ||||||||
| 48 | 76 | 103 | 104 | 111 | 159 | |||||||
| 68 | 76 | 103 | 104 | 159 | 236 | |||||||
| 42 | 76 | 103 | 104 | 159 | ||||||||
| 12 | 62 | 76 | 103 | 104 | 159 | |||||||
| 42 | 76 | 103 | 104 | 159 | ||||||||
| 76 | 103 | 104 | 146 | 159 | ||||||||
| 76 | 103 | 104 | 159 | 238 | ||||||||
| 76 | 103 | 104 | 159 | 224 | ||||||||
| 76 | 103 | 104 | 212 | 268 | 271 | |||||||
| 76 | 89 | 103 | 104 | |||||||||
| 76 | 87 | 103 | 104 | 212 | 271 | |||||||
| 76 | 103 | 104 | 212 | 245 | 271 | |||||||
| 76 | 103 | 104 | 134 | 141 | 212 | 271 | ||||||
| 76 | 103 | 104 | 212 | 236 | 243 | 271 | ||||||
| 76 | 103 | 104 | 109 | 245 | ||||||||
| 76 | 103 | 104 | 109 | 210 | ||||||||
| 20 | 62 | 76 | 103 | 104 | ||||||||
| 68 | 76 | 103 | 104 | 236 | ||||||||
| 68 | 76 | 103 | 104 | 159 | 236 | 271 | ||||||
| 68 | 76 | 103 | 104 | 159 | 236 | 245 | ||||||
| 68 | 76 | 103 | 104 | 159 | 217 | 236 | 271 | |||||
| 17 | 68 | 76 | 103 | 104 |
PL 191 529 B1 ciąg dalszy tabeli 1
| 68 | 76 | 103 | 104 | |||||||||
| 68 | 76 | 103 | 104 | 159 | 236 | |||||||
| 68 | 75 | 76 | 103 | 104 | 159 | 236 | ||||||
| 68 | 76 | 76 | 103 | 114 | 121 | 159 | 236 | 245 | ||||
| 12 | 68 | 76 | 103 | 104 | 159 | 236 | ||||||
| 68 | 76 | 103 | 104 | 159 | 209 | 236 | 253 | |||||
| 68 | 76 | 103 | 104 | 117 | 159 | 184 | 236 | |||||
| 68 | 76 | 103 | 104 | 159 | 236 | 243 | ||||||
| 68 | 76 | 103 | 104 | 159 | 236 | 245 | ||||||
| 68 | 76 | 103 | 104 | 142 | 159 | |||||||
| 68 | 76 | 103 | 104 | 123 | 159 | 236 | 249 | |||||
| 68 | 76 | 103 | 104 | 159 | 236 | 249 | ||||||
| 76 | 103 | 104 | 222 | 245 | ||||||||
| 12 | 76 | 103 | 104 | 222 | 249 | |||||||
| 76 | 103 | 104 | 173 | 222 | ||||||||
| 76 | 103 | 104 | 222 | 263 | ||||||||
| 21 | 76 | 103 | 104 | 222 | 237 | 263 | ||||||
| 76 | 103 | 104 | 109 | 222 | ||||||||
| 76 | 103 | 104 | 109 | 222 | 271 | |||||||
| 61 | 76 | 103 | 104 | 222 | ||||||||
| 76 | 103 | 104 | 137 | 222 | ||||||||
| 76 | 103 | 104 | 109 | 222 | 248 | |||||||
| 76 | 103 | 104 | 222 | 249 | ||||||||
| 68 | 76 | 103 | 104 | 159 | 236 | 245 | 261 | |||||
| 68 | 76 | 103 | 104 | 141 | 159 | 236 | 245 | 255 | ||||
| 68 | 76 | 103 | 104 | 159 | 236 | 245 | 247 | |||||
| 68 | 76 | 103 | 104 | 159 | 174 | 204 | 236 | 245 | ||||
| 68 | 76 | 103 | 104 | 159 | 204 | 236 | 245 | |||||
| 68 | 76 | 103 | 104 | 133 | 159 | 218 | 236 | 245 | ||||
| 68 | 76 | 103 | 104 | 159 | 232 | 236 | 245 | |||||
| 68 | 76 | 103 | 104 | 159 | 194 | 203 | 236 | 245 | ||||
| 12 | 76 | 103 | 104 | 222 | 245 | |||||||
| 76 | 103 | 104 | 232 | 245 | ||||||||
| 24 | 68 | 76 | 103 | 104 | 159 | 232 | 236 | 245 | ||||
| 68 | 103 | 104 | 159 | 232 | 236 | 245 | 252 | |||||
| 68 | 76 | 103 | 104 | 159 | 213 | 232 | 236 | 245 | 260 | |||
| 12 | 76 | 103 | 104 | 222 | 244 | 245 |
PL 191 529 B1 ciąg dalszy tabeli 1
| 12 | 76 | 103 | 222 | 210 | 245 | |||||||
| 12 | 76 | 103 | 104 | 130 | 222 | 245 | ||||||
| 22 | 68 | 76 | 103 | 104 | ||||||||
| 68 | 76 | 103 | 104 | 184 | ||||||||
| 68 | 103 | 104 | 159 | 232 | 236 | 245 | 248 | 252 | ||||
| 68 | 103 | 104 | 159 | 232 | 236 | 245 | ||||||
| 68 | 103 | 104 | 140 | 159 | 232 | 236 | 245 | 252 | ||||
| 43 | 68 | 103 | 104 | 159 | 232 | 236 | 245 | 252 | ||||
| 43 | 68 | 103 | 104 | 159 | 232 | 236 | 245 | |||||
| 43 | 68 | 103 | 104 | 159 | 232 | 236 | 245 | 252 | ||||
| 68 | 87 | 103 | 104 | 159 | 232 | 236 | 245 | 252 | 275 | |||
| 12 | 76 | 103 | 104 | 130 | 222 | 245 | 248 | 262 | ||||
| 12 | 76 | 103 | 104 | 130 | 215 | 222 | 245 | |||||
| 12 | 76 | 103 | 104 | 130 | 222 | 227 | 245 | 262 | ||||
| 12 | 76 | 103 | 104 | 130 | 222 | 245 | 261 | |||||
| 76 | 103 | 104 | 130 | 222 | 245 | |||||||
| 12 | 76 | 103 | 104 | 130 | 218 | 222 | 245 | 262 | 269 | |||
| 12 | 57 | 76 | 103 | 104 | 130 | 222 | 245 | 251 | ||||
| 12 | 76 | 103 | 104 | 130 | 170 | 185 | 222 | 243 | 245 | |||
| 12 | 76 | 103 | 104 | 130 | 222 | 245 | 268 | |||||
| 12 | 76 | 103 | 104 | 130 | 222 | 210 | 245 | |||||
| 68 | 103 | 104 | 159 | 232 | 236 | 245 | 257 | |||||
| 68 | 103 | 104 | 116 | 159 | 232 | 236 | 245 | |||||
| 68 | 103 | 104 | 159 | 232 | 236 | 245 | 248 | |||||
| 10 | 68 | 103 | 104 | 159 | 232 | 236 | 245 | |||||
| 68 | 103 | 104 | 159 | 203 | 232 | 236 | 245 | |||||
| 68 | 103 | 104 | 159 | 232 | 236 | 237 | 245 | |||||
| 68 | 76 | 79 | 103 | 104 | 159 | 232 | 236 | 245 | ||||
| 68 | 103 | 104 | 159 | 183 | 232 | 236 | 245 | |||||
| 68 | 103 | 104 | 159 | 174 | 206 | 232 | 236 | 245 | ||||
| 68 | 103 | 104 | 159 | 188 | 232 | 236 | 245 | |||||
| 68 | 103 | 104 | 159 | 230 | 232 | 236 | 245 | |||||
| 68 | 98 | 103 | 104 | 159 | 232 | 236 | 245 | |||||
| 68 | 103 | 104 | 159 | 215 | 232 | 236 | 245 | |||||
| 68 | 103 | 104 | 159 | 232 | 236 | 245 | 248 | |||||
| 68 | 76 | 103 | 104 | 159 | 232 | 236 | 245 | |||||
| 68 | 76 | 103 | 104 | 159 | 210 | 232 | 236 | 245 |
PL 191 529 B1 ciąg dalszy tabeli 1
| 68 | 76 | 103 | 104 | 159 | 232 | 236 | 245 | 257 | ||||
| 76 | 103 | 104 | 232 | 236 | 245 | 257 | ||||||
| 68 | 103 | 104 | 159 | 232 | 236 | 245 | 257 | 275 | ||||
| 76 | 103 | 104 | 257 | 275 | ||||||||
| 68 | 103 | 104 | 159 | 224 | 232 | 236 | 245 | 257 | ||||
| 76 | 103 | 104 | 159 | 232 | 236 | 245 | 257 | |||||
| 68 | 76 | 103 | 104 | 159 | 209 | 232 | 236 | 245 | ||||
| 68 | 76 | 103 | 104 | 159 | 211 | 232 | 236 | 245 | ||||
| 12 | 68 | 76 | 103 | 104 | 159 | 214 | 232 | 236 | 245 | |||
| 68 | 76 | 103 | 104 | 159 | 215 | 232 | 236 | 245 | ||||
| 12 | 68 | 76 | 103 | 104 | 159 | 232 | 236 | 245 | ||||
| 20 | 68 | 76 | 103 | 104 | 159 | 232 | 236 | 245 | 259 | |||
| 68 | 87 | 76 | 103 | 104 | 159 | 232 | 236 | 245 | 260 | |||
| 68 | 76 | 103 | 104 | 169 | 232 | 236 | 245 | 261 | ||||
| 76 | 103 | 104 | 232 | 236 | 242 | 245 | ||||||
| 68 | 76 | 103 | 104 | 159 | 210 | 232 | 236 | 245 | ||||
| 12 | 48 | 68 | 76 | 103 | 104 | 159 | 232 | 236 | 245 | |||
| 76 | 103 | 104 | 232 | 236 | 245 | |||||||
| 76 | 103 | 104 | 159 | 192 | 232 | 236 | 245 | |||||
| 76 | 103 | 104 | 147 | 159 | 232 | 236 | 245 | 248 | 251 | |||
| 12 | 68 | 76 | 103 | 104 | 159 | 232 | 236 | 245 | 272 | |||
| 68 | 76 | 103 | 104 | 159 | 183 | 206 | 232 | 236 | 245 | |||
| .68 | 76 | 103 | 104 | 159 | 232 | 236 | 245 | 256 | ||||
| 68 | 76 | 103 | 104 | 159 | 206 | 232 | 236 | 245 | ||||
| 27 | 68 | 76 | 103 | 104 | 159 | 232 | 236 | 245 | ||||
| 68 | 76 | 103 | 104 | 116 | 159 | 170 | 185 | 232 | 236 | 245 | ||
| 61 | 68 | 103 | 104 | 159 | 232 | 236 | 245 | 248 | 252 | |||
| 43 | 68 | 103 | 104 | 159 | 232 | 236 | 245 | 248 | 252 | |||
| 68 | 103 | 104 | 159 | 212 | 232 | 236 | 245 | 248 | 252 | |||
| 68 | 103 | 104 | 99 | 159 | 184 | 232 | 236 | 245 | 248 | 252 | ||
| 103 | 104 | 159 | 232 | 236 | 245 | 248 | 252 | |||||
| 68 | 103 | 104 | 159 | 209 | 232 | 236 | 245 | 248 | 252 | |||
| 68 | 103 | 104 | 109 | 159 | 232 | 236 | 245 | 248 | 252 | |||
| 20 | 68 | 103 | 104 | 159 | 232 | 236 | 245 | 248 | 252 | |||
| 68 | 103 | 104 | 159 | 309 | 232 | 236 | 245 | 248 | 252 | |||
| 68 | 103 | 104 | 159 | 232 | 236 | 245 | 248 | 252 | 261 | |||
| 68 | 103 | 104 | 159 | 185 | 232 | 236 | 245 | 248 | 252 |
PL 191 529 B1 ciąg dalszy tabeli 1
| 68 | 103 | 104 | 159 | 210 | 232 | 236 | 245 | 248 | 252 | |||
| 68 | 103 | 104 | 159 | 185 | 210 | 232 | 236 | 245 | 248 | 252 | ||
| 68 | 103 | 104 | 159 | 212 | 232 | 236 | 245 | 248 | 252 | |||
| 68 | 103 | 104 | 159 | 213 | 232 | 236 | 245 | 248 | 252 | |||
| 68 | 103 | 104 | 213 | 232 | 236 | 245 | 248 | 252 | ||||
| 68 | 103 | 104 | 159 | 215 | 232 | 236 | 245 | 248 | 252 | |||
| 68 | 103 | 104 | 159 | 216 | 232 | 236 | 245 | 248 | 252 | |||
| 20 | 68 | 103 | 104 | 159 | 232 | 236 | 245 | 248 | 252 | |||
| 68 | 103 | 104 | 159 | 173 | 232 | 236 | 245 | 248 | 252 | |||
| 68 | 103 | 104 | 159 | 232 | 236 | 245 | 248 | 251 | 252 | |||
| 68 | 103 | 104 | 159 | 206 | 232 | 236 | 245 | 248 | 252 | |||
| 68 | 103 | 104 | 159 | 232 | 236 | 245 | 248 | 252 | ||||
| 55 | 68 | 103 | 104 | 159 | 232 | 236 | 245 | 248 | 252 | |||
| 68 | 103 | 104 | 159 | 232 | 236 | 245 | 248 | 252 | 255 | |||
| 68 | 103 | 104 | 159 | 232 | 236 | 245 | 248 | 252 | 256 | |||
| 68 | 103 | 104 | 159 | 232 | 236 | 245 | 248 | 252 | 260 | |||
| 68 | 103 | 104 | 159 | 232 | 236 | 245 | 248 | 252 | 257 | |||
| 68 | 103 | 104 | 159 | 232 | 236 | 245 | 248 | 252 | 258 | |||
| 8 | 68 | 103 | 104 | 159 | 232 | 236 | 245 | 248 | 252 | 269 | ||
| 68 | 103 | 104 | 116 | 159 | 232 | 236 | 245 | 248 | 252 | 260 | ||
| 68 | 103 | 104 | 159 | 232 | 236 | 245 | 248 | 252 | 261 | |||
| 68 | 103 | 104 | 159 | 232 | 236 | 245 | 248 | 252 | 261 | |||
| 68 | 76 | 103 | 104 | 159 | 232 | 236 | 245 | 248 | 252 | |||
| 68 | 103 | 104 | 232 | 236 | 245 | 248 | 252 | |||||
| 103 | 104 | 159 | 232 | 236 | 245 | 248 | 252 | |||||
| 68 | 103 | 104 | 159 | 232 | 236 | 245 | 248 | 252 | ||||
| 18 | 68 | 103 | 104 | 159 | 232 | 236 | 245 | 248 | 252 | |||
| 68 | 103 | 104 | 159 | 232 | 236 | 245 | 248 | 252 | ||||
| 68 | 76 | 101 | 103 | 104 | 159 | 213 | 218 | 232 | 236 | 245 | 260 | |
| 68 | 103 | 104 | 159 | 228 | 232 | 236 | 245 | 248 | 252 | |||
| 33 | 68 | 76 | 103 | 104 | 159 | 232 | 236 | 245 | 248 | 252 | ||
| 68 | 76 | 89 | 103 | 104 | 159 | 210 | 213 | 232 | 236 | 245 | 260 | |
| 61 | 68 | 76 | 103 | 104 | 159 | 232 | 236 | 245 | 248 | 252 | ||
| 103 | 104 | 159 | 205 | 210 | 232 | 236 | 245 | |||||
| 61 | 68 | 103 | 104 | 130 | 159 | 232 | 236 | 245 | 248 | 252 | ||
| 61 | 68 | 103 | 104 | 133 | 137 | 159 | 232 | 236 | 245 | 248 | 252 | |
| 61 | 103 | 104 | 133 | 159 | 232 | 236 | 245 | 248 | 252 |
PL 191 529 B1 ciąg dalszy tabeli 1
| 68 | 103 | 104 | 159 | 232 | 236 | 245 | 248 | 252 | ||||
| 68 | 103 | 104 | 159 | 218 | 232 | 236 | 245 | 248 | 252 | |||
| 61 | 68 | 103 | 104 | 159 | 160 | 232 | 236 | 245 | 248 | 252 | ||
| 3 | 61 | 68 | 76 | 103 | 104 | 232 | 236 | 245 | 248 | 252 | ||
| 61 | 68 | 103 | 104 | 159 | 167 | 232 | 236 | 245 | 248 | 252 | ||
| 97 | 103 | 104 | 159 | 232 | 236 | 245 | 248 | 252 | ||||
| 98 | 103 | 104 | 159 | 232 | 236 | 245 | 248 | 252 | ||||
| 99 | 103 | 104 | 159 | 232 | 236 | 245 | 248 | 252 | ||||
| 101 | 103 | 104 | 159 | 232 | 236 | 245 | 248 | 252 | ||||
| 102 | 103 | 104 | 159 | 232 | 236 | 245 | 248 | 252 | ||||
| 103 | 104 | 106 | 159 | 232 | 236 | 245 | 248 | 252 | ||||
| 103 | 104 | 109 | 159 | 232 | 236 | 245 | 248 | 252 | ||||
| 103 | 104 | 159 | 232 | 236 | 245 | 248 | 252 | 261 | ||||
| 62 | 103 | 104 | 159 | 232 | 236 | 245 | 248 | 252 | ||||
| 103 | 104 | 159 | 184 | 232 | 236 | 245 | 248 | 252 | ||||
| 103 | 104 | 159 | 166 | 232 | 236 | 245 | 248 | 252 | ||||
| 103 | 104 | 159 | 217 | 232 | 236 | 245 | 248 | 252 | ||||
| 20 | 62 | 103 | 104 | 159 | 213 | 232 | 236 | 245 | 248 | 252 | ||
| 62 | 103 | 104 | 159 | 213 | 232 | 236 | 245 | 248 | 252 | |||
| 103 | 104 | 159 | 206 | 217 | 232 | 236 | 245 | 248 | 252 | |||
| 62 | 103 | 104 | 159 | 206 | 232 | 236 | 245 | 248 | 252 | |||
| 103 | 104 | 130 | 159 | 232 | 236 | 245 | 248 | 252 | ||||
| 103 | 104 | 131 | 159 | 232 | 236 | 245 | 248 | 252 | ||||
| 27 | 103 | 104 | 159 | 2-32 | 236 | 245 | 248 | 252 | ||||
| 38 | 103 | 104 | 159 | 232 | 236 | 245 | 248 | 252 | ||||
| 38 | 76 | 103 | 104 | 159 | 213 | 232 | 236 | 245 | 260 | |||
| 68 | 76 | 103 | 104 | 159 | 213 | 232 | 236 | 245 | 260 | 271 | ||
| 68 | 76 | 103 | 104 | 159 | 209 | 213 | 232 | 236 | 245 | 260 | ||
| 68 | 76 | 103 | 104 | 159 | 210 | 213 | 232 | 236 | 245 | 260 | ||
| 68 | 76 | 103 | 104 | 159 | 205 | 213 | 232 | 236 | 245 | 260 | ||
| 68 | 76 | 103 | 104 | 159 | 210 | 232 | 236 | 245 | 260 | |||
| 68 | 103 | 104 | 159 | 213 | 232 | 236 | 245 | 260 | ||||
| 76 | 103 | 104 | 159 | 213 | 232 | 236 | 245 | 260 | ||||
| 68 | 103 | 104 | 159 | 209 | 232 | 236 | 245 | |||||
| 68 | 103 | 104 | 159 | 210 | 232 | 236 | 245 | |||||
| 68 | 103 | 104 | 159 | 230 | 232 | 236 | 245 | |||||
| 68 | 103 | 104 | 159 | 126 | 232 | 236 | 245 |
PL 191 529 B1 ciąg dalszy tabeli 1
| 68 | 103 | 104 | 159 | 205 | 232 | 236 | 245 | |||||
| 68 | 103 | 104 | 159 | 210 | 232 | 236 | 245 | |||||
| 103 | 104 | 159 | 230 | 236 | 245 | |||||||
| 68 | 103 | 104 | 159 | 232 | 236 | 245 | 260 | |||||
| 103 | 104 | 159 | 232 | 236 | 245 | |||||||
| 68 | 103 | 104 | 159 | 174 | 232 | 236 | 245 | 257 | ||||
| 68 | 103 | 104 | 159 | 194 | 232 | 236 | 245 | 257 | ||||
| 68 | 103 | 104 | 159 | 209 | 232 | 236 | 245 | 257 | ||||
| 103 | 104 | 159 | 232 | 236 | 245 | 257 | ||||||
| 68 | 76 | 103 | 104 | 159 | 213 | 232 | 236 | 245 | 260 | 261 | ||
| 68 | 103 | 104 | 159 | 232 | 236 | 245 | 257 | 261 | ||||
| 103 | 104 | 159 | 213 | 232 | 236 | 245 | 260 | |||||
| 103 | 104 | 159 | 210 | 232 | 236 | 245 | 248 | 252 | ||||
| 103 | 104 | 159 | 209 | 232 | 236 | 245 | 257 | |||||
| 68 | 76 | 103 | 104 | 159 | 210 | 213 | 232 | 236 | 245 | 260 | ||
| 12 | 103 | 104 | 159 | 209 | 213 | 232 | 236 | 245 | 260 | |||
| 103 | 104 | 209 | 232 | 236 | 245 | 257 | ||||||
| 103 | 104 | 159 | 205 | 210 | 213 | 232 | 236 | 245 | 260 | |||
| 103 | 104 | 159 | 205 | 209 | 232 | 236 | 245 | 260 | ||||
| 68 | 103 | 104 | 159 | 205 | 209 | 210 | 232 | 236 | 245 | |||
| 103 | 104 | 159 | 205 | 209 | 210 | 232 | 236 | 245 | 257 | |||
| 103 | 104 | 159 | 205 | 209 | 232 | 236 | 245 | 257 | ||||
| 68 | 103 | 104 | 159 | 205 | 209 | 210 | 232 | 236 | 245 | 260 | ||
| 103 | 104 | 159 | 205 | 209 | 210 | 232 | 236 | 245 | ||||
| 103 | 104 | 159 | 209 | 210 | 232 | 236 | 245 | |||||
| 103 | 104 | 159 | 205 | 210 | 232 | 236 | 245 | |||||
| 68 | 103 | 104 | 128 | 159 | 232 | 236 | 245 | |||||
| -48 | 103 | 104 | 159 | 230 | 236 | 245 | ||||||
| 48 | 68 | 103 | 104 | 169 | 209 | 232 | 236 | 245 | ||||
| 48 | 68 | 103 | 104 | 159 | 232 | 236 | 245 | 248 | 252 | |||
| 48 | 68 | 103 | 104 | 159 | 232 | 236 | 245 | 257 | 261 | |||
| 102 | 103 | 104 | 159 | 212 | 232 | 236 | 245 | 248 | 252 | |||
| 12 | 102 | 103 | 104 | 159 | 212 | 232 | 236 | 245 | 248 | 252 | ||
| 101 | 102 | 103 | 104 | 159 | 212 | 232 | 236 | 245 | 248 | 252 | ||
| 98 | 102 | 103 | 104 | 159 | 212 | 232 | 236 | 245 | 248 | 252 | ||
| 102 | 103 | 104 | 159 | 213 | 232 | 236 | 245 | 248 | 252 | |||
| 103 | 104 | 131 | 159 | 232 | 236 | 245 | 248 | 252 |
PL 191 529 B1 ciąg dalszy tabeli 1
| 103 | 104 | 159 | 184 | 232 | 236 | 245 | 248 | 252 | ||||
| 103 | 104 | 159 | 232 | 236 | 244 | 245 | 248 | 252 | ||||
| 62 | 103 | 104 | 159 | 213 | 232 | 236 | 245 | 248 | 252 | 256 | ||
| 12 | 62 | 103 | 104 | 159 | 213 | 232 | 236 | 245 | 248 | 252 | ||
| 101 | 103 | 104 | 159 | 185 | 232 | 236 | 245 | 248 | 252 | |||
| 101 | 103 | 104 | 159 | 206 | 232 | 236 | 245 | 248 | 252 | |||
| 101 | 103 | 104 | 159 | 213 | 232 | 236 | 245 | 248 | 252 | |||
| 98 | 102 | 103 | 104 | 159 | 232 | 236 | 245 | 248 | 252 | |||
| 101 | 102 | 103 | 104 | 159 | 232 | 236 | 245 | 248 | 252 | |||
| 98 | 102 | 103 | 104 | 159 | 212 | 232 | 236 | 245 | 248 | 252 | ||
| 98 | 102 | 103 | 104 | 159 | 212 | 232 | 236 | 248 | 252 | |||
| 62 | 103 | 104 | 109 | 159 | 213 | 232 | 236 | 245 | 248 | 252 | ||
| 62 | 103 | 104 | 159 | 212 | 213 | 232 | 236 | 245 | 248 | 252 | ||
| 62 | 101 | 103 | 104 | 159 | 212 | 213 | 232 | 236 | 245 | 248 | 252 | |
| 103 | 104 | 159 | 232 | 245 | 248 | 252 | ||||||
| 103 | 104 | 159 | 230 | 245 | ||||||||
| 62 | 103 | 104 | 130 | 159 | 213 | 232 | 236 | 245 | 248 | 252 | ||
| 101 | 103 | 104 | 130 | 159 | 232 | 236 | 245 | 248 | 252 | |||
| 101 | 103 | 104 | 128 | 159 | 232 | 236 | 245 | 248 | 252 | |||
| 62 | 101 | 103 | 104 | 159 | 213 | 232 | 236 | 245 | 248 | 252 | ||
| 62 | 103 | 104 | 128 | 159 | 213 | 232 | 236 | 245 | 248 | 252 | ||
| 62 | 103 | 104 | 128 | 159 | 213 | 232 | 236 | 245 | 248 | 252 | ||
| 101 | 103 | 104 | 159 | 232 | 236 | 245 | 248 | 252 | 260 | |||
| 101 | 103 | 104 | 131 | 159 | 232 | 236 | 245 | 248 | 252 | |||
| 98 | 101 | 103 | 104 | 159 | 232 | 236 | 245 | 248 | 252 | |||
| 99 | 101 | 103 | 104 | 159 | 232 | 236 | 245 | 248 | 252 | |||
| 101 | 103 | 104 | 159 | 212 | 232 | 236 | 245 | 248 | 252 | |||
| 101 | 103 | 104 | 159 | 209 | 232 | 236 | 245 | 248 | 252 | |||
| 101 | 103 | 104 | 159 | 210 | 232 | 236 | 245 | 248 | 252 | |||
| 101 | 103 | 104 | 159 | 205 | 232 | 236 | 245 | 248 | 252 | |||
| 101 | 103 | 104 | 159 | 230 | 236 | 245 | ||||||
| 101 | 103 | 104 | 159 | 194 | 232 | 236 | 245 | 248 | 252 | |||
| 76 | 101 | 103 | 104 | 159 | 194 | 232 | 236 | 245 | 248 | 252 | ||
| 101 | 103 | 104 | 159 | 230 | 232 | 236 | 245 | 248 | 252 | |||
| 62 | 103 | 104 | 159 | 185 | 206 | 213 | 232 | 236 | 245 | 248 | 252 | 271 |
PL 191 529 B1
Bardziej korzystne odmiany proteazy zawierają zestaw podstawień wybrany z grupy obejmującej pozycje reszt odpowiadających pozycjom subtylizyny z Bacillus amyloliquefaciens przedstawionym w tabeli 2.
T a b e l a 2
| N76D | S103A | V104l | M222S | |||||||||
| N76D | A98E | S103A | V104l | |||||||||
| N76D | S78T | S103A | Y104I | |||||||||
| N76D | S103A | V104l | I107Y | |||||||||
| V4E | N76D | S103A | V104l | |||||||||
| N76D | S103A | V104l | I246Y | |||||||||
| N76D | N77D | S103A | V104J | |||||||||
| N76D | S103A | V104l | N183D | N218I | ||||||||
| A16T | N76D | S103A | V104l | N248D | ||||||||
| A1E | N76D | S103A | V104l | |||||||||
| N76D | S103A | V104l | N261D | |||||||||
| N76D | S103A | V104l | S160T | |||||||||
| N76D | S103A | Y104I | S216C | |||||||||
| H17Q | N76D | S103A | V104l | |||||||||
| S37T | N76D | S103A | V104l | |||||||||
| N76D | N77D | S103A | V104l | A174V | ||||||||
| T38S | N76D | S103A | V104l | |||||||||
| T38S | N76D | S103A | V104l | K237Q | ||||||||
| I8V | N76D | S103A | V104l | |||||||||
| N76D | S103A | V 1041 | N183D | |||||||||
| R19L | N76D | S103A | V104l | |||||||||
| A13V | N76D | S103A | V104l | |||||||||
| R19C | N76D | S103A | V104l | |||||||||
| N76D | S103A | V104l | N184D | |||||||||
| N76D | S103A | V104l | N252D | |||||||||
| N76D | S103A | V104l | S259C | |||||||||
| N76D | S103A | V104l | K251T | |||||||||
| N76D | P86S | S103A | V104l | |||||||||
| I72V | N76D | S103A | V104l | N185D | ||||||||
| N76D | S103A | V104l | K237E | T274A | ||||||||
| N76D | S103A | V104l | S160L | |||||||||
| N76D | S103A | V104l | A228Y | |||||||||
| P55S | N76D | S103A | V104l | S240T |
PL 191 529 B1 ciąg dalszy tabeli 2
| N76D | S103A | V104l | A254T | |||||||||
| N76D | S103A | I104N | N204T | |||||||||
| N76D | S103A | V104l | N204D | |||||||||
| N43S | N76D | S103A | V104l | |||||||||
| N76D | S103A | V104i | G159D | |||||||||
| R10H | N76D | S103A | V104l | Y177A | ||||||||
| T58S | N76D | S103A | V104l | |||||||||
| N76D | S103A | V104l | A270Y | |||||||||
| N76D | S103A | V104l | N185D | |||||||||
| Κ27Ν | N76D | S103A | V104l | |||||||||
| N76D | S103A | V104l | L262M | |||||||||
| N76D | S78P | S103A | V104l | |||||||||
| S24P | N76D | S103A | V104l | |||||||||
| N76D | S103A | V104l | S166G | Q236R | K251R | |||||||
| H17L | N76D | S103A | V104l | K237E | ||||||||
| N76D | S103A | V104l | S130L | |||||||||
| N76D | S103A | V104l | Q109R | |||||||||
| N76D | S99R | S103A | V104l | N204T | ||||||||
| N76D | S103A | V104l | D181N | |||||||||
| Q12R | N76D | S103A | V104l | |||||||||
| N76D | S103A | V104l | S212P | E271V | ||||||||
| N76D | S103A | V104l | N252K | N261Y | ||||||||
| N76D | S103A | V104l | S242T | |||||||||
| N76D | S103A | V104l | E271Q | |||||||||
| Q12R | N76D | S103A | V104l | S242T | ||||||||
| N43S | N76D | S103A | V104l | N116K | N1831 | |||||||
| N76D | S103A | V104l | G258R | |||||||||
| N76D | S103A | V104l | E271G | |||||||||
| G61R | N76D | S103A | V104l | |||||||||
| T38S | N76D | S103A | V104l | Q182R | Y263H | |||||||
| N76D | S103A | V104l | Q182R | A272S | ||||||||
| N76D | S103A | V104l | Q109R | I246Y | ||||||||
| N76D | S87G | S103A | V104l | Q206R | H249Q | S265G | ||||||
| N76D | S103A | V104l | Q137R | N238Y | E271V | |||||||
| S103A | V104l | A228T | ||||||||||
| N76D | S103A | V104l | Q182R | 1198V | ||||||||
| L21M | N76D | S103A | V104l | Q182R |
PL 191 529 B1 ciąg dalszy tabeli 2
| N76D | S103A | V104l | M119I | Q137R | ||||||||
| N76D | S103A | V104l | Q137R | N248S | ||||||||
| Α13Τ | N76D | S103A | V104) | Q206R | ||||||||
| N76D | S103A | V104l | Q206R | |||||||||
| N76D | S103A | Y104I | 8212P | G258R | ||||||||
| T58S | N76D | S103A | V104l | E271G | ||||||||
| N76D | S103A | V104l | Q206E | N261D | ||||||||
| V4E | N76D | S103A | V104l | Q206E | ||||||||
| N76D | N77D | S103A | V104l | Q206E | ||||||||
| N76D | S103A | V104l | A158E | |||||||||
| N76D | S103A | V104l | Q206E | |||||||||
| V4E | N76D | S103A | V104l | G159D | L217E | K251Q | ||||||
| V4E | N76D | S103A | V104l | G159D | L217E | N252D | ||||||
| N76D | N77D | S103A | V104l | A1SST | N185D | K251T | ||||||
| N76D | S103A | V104l | G159D | Q206E | Y244A | |||||||
| V4E | N76D | S103A | V104l | 8188E | ||||||||
| V4E | N76D | S103A | V104l | A158E | ||||||||
| N76D | N77D | S103A | V104l | N185D | ||||||||
| N76D | S103A | V104l | Q206E | K251T | ||||||||
| A48T | N76D | S103A | V104l | L111M | G159D | |||||||
| V68A | N76D | S103A | V104l | G159D | Q2S6H | |||||||
| L42V | N76D | S103A | V104l | G159D | ||||||||
| Q12H | N62H | N76D | S103A | V104l | G159D | |||||||
| L421 | N76D | S103A | V104l | G159D | ||||||||
| N76D | S103A | Y104I | G146S | G159D | ||||||||
| N76D | S103A | V104l | G159D | N238S | ||||||||
| N76D | S103A | Y104I | G159D | T224A | ||||||||
| N76D | S103A | V104l | S212P | Y268F | E271Y | |||||||
| N76D | E89A | S103A | V104l | |||||||||
| N76D | S87R | S103A | V104l | S212P | E271Y | |||||||
| N76D | S103A | V104l | S212P | Q245L | E271V | |||||||
| N76D | S103A | V104l | T1A4S | S141N | S212P | E271V | ||||||
| N76D | S103A | V104l | S212P | Q236L | N243S | E271V | ||||||
| N76D | S103A | V104l | Q109R | Q245R | ||||||||
| N76D | S103A | V104l | Q109R | P210L | ||||||||
| G20V | N62S | N76D | S103A | V104l | ||||||||
| V68A | N76D | S103A | V104l | Q236H |
PL 191 529 B1 ciąg dalszy tabeli 2
| V68A | N76D | S103A | V104l | G159D | Q236H | E271V | ||||||
| V68A | N76D | S103A | V104l | G159D | Q236H | Q245R | ||||||
| V68A | N76D | S103A | V104l | G159D | L217I | Q236H | E271V | |||||
| H17Q | V68A | N76D | S103A | V104l | ||||||||
| V68A | N76D | S103A | V104l | |||||||||
| V68A | N76D | S103A | V104l | G159D | Q236R | |||||||
| V68A | L75R | N76D | S103A | V104l | G159D | Q236H | ||||||
| V68A | N76D | N76D | S103A | A114Y | Y121I | G159D | Q236H | Q245R | ||||
| Q12R | V68A | N76D | S103A | V104l | G159D | Q236H | ||||||
| V68A | N76D | S103A | V104l | G159D | Y209S | Q236H | T253K | |||||
| V68A | N76D | S103A | V104l | N117K | G159D | N184S | Q236H | |||||
| V68A | N76D | S103A | V104l | G159D | Q236H | N243I | ||||||
| V68A | N76D | S103A | V104l | G159D | Q236H | Q245L | ||||||
| V68A | N76D | S103A | V104l | A142Y | G159D | |||||||
| V68A | N76D | S103A | V104l | N123S | G159D | Q236H | H249Y | |||||
| V68A | N76D | S103A | V104l | G159D | Q236H | H249Q | ||||||
| N76D | S103A | V104l | M222S | Q245R | ||||||||
| N76D | S103A | V104l | Q12R | M222S | H249R | |||||||
| N76D | S103A | V104l | N173R | M222S | ||||||||
| N76D | S103A | V104l | M222S | Y263F | ||||||||
| L21M | N76D | S103A | V104l | M222S | K237R | Y263F | ||||||
| N76D | S103A | V104l | Q109R | M222S | ||||||||
| N76D | S103A | Y104I | Q109R | M222S | E271D | |||||||
| G61R | N76D | S103A | V104l | M222S | ||||||||
| N76D | S103A | V104l | Q137R | M222S | ||||||||
| N76D | S103A | V104l | Q109R | M222S | N248S | |||||||
| N76D | S103A | Y104I | M222S | H249R | ||||||||
| V68A | N76D | S103A | V104i | G159D | Q236H | Q245R | N261D | |||||
| V68A | N76D | S103A | V104l | S141N | G159D | Q236H | Q245R | T255S | ||||
| V68A | N76D | S103A | V104l | G159D | Q236H | Q245R | R247H | |||||
| V68A | N76D | S103A | V104l | G159D | A174V | N204D | Q236H | Q245R | ||||
| V68A | N76D | S103A | V104l | G159D | N204D | Q236H | Q245R | |||||
| V68A | N76D | S103A | V104l | A133V | G159D | N218D | Q236H | Q245R | ||||
| V68A | N76D | S103A | V104l | G159D | A232V | Q236H | Q245R | |||||
| V68A | N76D | S103A | V104l | G159D | A194I | Y203A | Q236H | Q245R | ||||
| Q12R | N76D | S103A | V104l | M222S | Q245R | |||||||
| N76D | S103A | Y104I | A232Y | Q245R |
PL 191 529 B1 ciąg dalszy tabeli 2
| S24T | V68A | N76D | S103A | V104l | G159D | A232V | Q236H | Q245R | ||||
| V68A | S103A | V104l | G159D | A232V | Q236H | Q245R | N252K | |||||
| V68A | N76D | S103A | V104l | G159D | T213R | A232V | Q236H | G245R | T260A | |||
| Q12R | N76D | S103A | I104T | M222S | Y244I | Q245R | ||||||
| Q12R | N76D | S103A | M222S | P210T | Q245R | |||||||
| Q12R | N76D | S103A | I104T | S130T | M222S | Q245R | ||||||
| T22K | V68A | N76D | S103A | V104l | ||||||||
| V68A | N76D | S103A | N184D | |||||||||
| V68A | S103A | V104l | G159D | A232V | Q236H | Q245R | N248D | N252K | ||||
| V68A | S103A | Y104I | G159D | A232Y | Q236H | Q245R | ||||||
| V68A | S103A | V104l | N140D | G159D | A232V | Q236H | Q245R | N252K | ||||
| N43S | V68A | S103A | V104l | G159D | A232V | Q236H | Q245R | N252K | ||||
| N43K | V68A | S103A | V104l | G159D | A232V | Q236H | Q245R | |||||
| N43D | V68A | S103A | V104l | G159D | A232V | Q236H | Q245R | N252K | ||||
| V68A | S87G | S103A | V104l | G159D | A232V | Q236H | Q245R | N252K | R275S | |||
| Q12R | N76D | S103A | I104T | S130T | M222S | Q245R | N248S | L262M | ||||
| Q12R | N76D | S103A | I104T | S130T | A215Y | M222S | Q245R | |||||
| Q12R | N76D | S103A | I104T | S130T | M222S | V227A | Q245R | L262S | ||||
| Q12R | N76D | S103A | I104T | S130T | A215T | M222S | Q245R | |||||
| Q12R | N76D | S103A | I104T | S130T | M222S | Q245R | N261D | |||||
| N76D | S103A | I104T | S130T | M222S | Q245R | |||||||
| Q12R | N76D | S103A | I104T | S130T | N218D | M222S | Q245R | L262S | N269D | |||
| Q12R | S57P | N76D | S103A | I104T | S130T | M222S | Q245R | K251Q | ||||
| Q12R | N76D | S103A | I104T | S130T | R170S | N185D | M222S | N243D | O245R | |||
| Q12R | N76D | S103A | I104T | S130T | M222S | Q245R | Y268A | |||||
| Q12R | N76D | S103A | I104T | S130T | M222S | P210S | Q245R | |||||
| V68A | S103A | V104l | G159D | A232Y | Q236H | Q245R | L257V | |||||
| V68A | S103A | V104l | N116D | G159D | A232Y | Q236H | Q245R | |||||
| V68A | S103A | V104l | G159D | A232Y | Q236H | Q245R | N248D | |||||
| R10C | V68A | S103A | V104l | G159D | A232Y | Q236H | Q245R | |||||
| V68A | S103A | V104l | G159D | Y203E | A232Y | Q236H | Q245R | |||||
| V68A | S103A | V104l | G159D | A232Y | Q236H | K237E | Q245R | |||||
| V68A | N76D | 179N | S103A | V104l | G159D | A232Y | Q236H | Q245R | ||||
| V68A | S103A | V104l | G159D | N183D | A232Y | Q236H | Q245R | |||||
| V68A | S103A | V104l | G159D | A174Y | Q206L | A232Y | Q236H | Q245R | ||||
| V68A | S103A | V104l | G159D | S188C | A232Y | Q236H | Q245R | |||||
| V68A | S103A | V104l | G159D | A230T | A232Y | Q236H | Q245R |
PL 191 529 B1 ciąg dalszy tabeli 2
| V68A | A98T | S103A | V104l | G159D | A232Y | Q236H | Q245R | |||||
| V68A | S103A | V104l | G159D | A215T | A232Y | Q236H | Q245R | |||||
| V68A | S103A | V104l | G159D | A232Y | Q236H | Q245R | N248S | |||||
| V68A | N76D | S103A | V104l | G159D | A232Y | Q236H | Q245R | |||||
| V68A | N76D | S103A | V104l | G159D | P210R | A232Y | Q236H | Q245R | ||||
| V68A | N76D | S103A | V104l | G159D | A232Y | Q236H | Q245R | L257V | ||||
| N76D | S103A | V104l | A232Y | Q236H | Q245R | L257V | ||||||
| V68A | S103A | V104l | G159D | A232Y | Q236H | Q245R | L257Y | R275H | ||||
| N76D | S103A | V104l | L257Y | R275H | ||||||||
| V68A | S103A | V104l | G159D | T224A | A232Y | Q236H | Q245R | L257Y | ||||
| N76D | S103A | V104l | G159D | A232Y | Q236H | Q245R | L257Y | |||||
| V68A | N76D | S103A | V104l | G159D | Y209W | A232V | Q236H | Q245R | ||||
| V68A | N76D | S103A | V104l | G159D | G211R | A232V | Q236H | Q245R | ||||
| V68A | N76D | S103A | V104l | G159D | G211Y | A232V | Q236H | Q245R | ||||
| Q12R | V68A | N76D | S103A | V104l | G159D | Y214L | A232V | Q236H | Q245R | |||
| V68A | N76D | S103A | V104l | G159D | A215R | A232V | Q236H | Q245R | ||||
| Q12R | V68A | N76D | S103A | V1Q4I | G159D | A232V | Q236H | Q245R | ||||
| G20R | V68A | N76D | S103A | V104l | G159D | A232V | Q236H | Q245R | S259G | |||
| V68A | S87R | N76D | S103A | V104l | G159D | A232V | Q236H | Q245R | T260Y | |||
| V68A | N76D | S103A | V104l | G159D | A232V | Q236H | Q245R | N261G | ||||
| V68A | N76D | S103A | V104l | G159D | A232Y | Q236H | Q245R | N261W | ||||
| N76D | S103A | V104l | A232Y | Q236H | S242P | Q245R | ||||||
| V68A | N76D | S103A | V104l | G159D | P210L | A232V | Q236H | Q245R | ||||
| Q12R | A48Y | V68A | N76D | S103A | V104l | G159D | A232V | Q236H | Q245R | |||
| N76D | S103A | V104l | A232Y | Q236H | Q245R | |||||||
| N76D | S103A | V104l | G159D | Y192F | A232V | Q236H | Q245R | |||||
| V68A | S103A | V104l | G159D | A232Y | Q236H | Q245R | L257Y | R275H | ||||
| N76D | S103A | V104l | L257Y | R275H | ||||||||
| V68A | S103A | V104l | G159D | T224A | A232Y | Q236H | Q245R | L257V | ||||
| N76D | S103A | V104l | G159D | A232Y | Q236H | Q245R | L257V | |||||
| V68A | N76D | S103A | V104l | G159D | Y209W | A232V | Q236H | Q245R | ||||
| V68A | N76D | S103A | V104l | G159D | G211R | A232V | Q236H | Q245R | ||||
| V68A | N76D | S103A | V104l | G159D | G211Y | A232V | Q236H | Q245R | ||||
| Q12R | V68A | N76D | S103A | V104l | G159D | Y214L | A232V | Q236H | Q245R | |||
| V68A | N76D | S103A | VfÓ4l | G159D | A215R | A232V | Q236H | Q245R | ||||
| Q12R | V68A | N76D | S103A | V104l | G159D | A232V | Q236H | Q245R | ||||
| G20R | V68A | N76D | S103A | V104l | G159D | A232V | Q236H | Q245R | S259G |
PL 191 529 B1 ciąg dalszy tabeli 2
| V68A | S87R | N76D | S103A | V104l | G159D | A232V | Q236H | Q245R | T260Y | |||
| V68A | N76D | S103A | V104l | G159D | A232V | Q236H | Q245R | N261G | ||||
| V68A | N76D | S103A | V104l | G159D | A232Y | Q236H | Q245R | N261W | ||||
| N76D | S103A | V104l | A232Y | Q236H | S242P | Q245R | ||||||
| V68A | N76D | S103A | V104l | G159D | P210L | A232V | Q236H | Q245R | ||||
| Q12R | A48V | V68A | N76D | S103A | V104l | G159D | A232V | Q236H | Q245R | |||
| N76D | S103A | V104l | A232Y | Q236H | Q245R | |||||||
| N76D | S103A | V104l | G159D | Y192F | A232V | Q236H | Q245R | |||||
| N76D | S103A | V104l | Y147I | G159D | A232V | Q236H | Q245R | N248S | K251R | |||
| Q12R | V68A | N76D | S103A | V104l | G159D | A232V | Q236H | Q245R | A272S | |||
| V68A | N76D | S103A | V104l | G159D | N183K | Q206L | A232V | Q236H | Q245R | |||
| V68A | N76D | S103A | V104l | G159D | A232V | Q236H | Q245R | S256R | ||||
| V68A | N76D | S103A | V104l | G159D | Q206R | A232V | Q236H | Q245R | ||||
| K27R | V68A | N76D | S103A | V104l | G159D | A232V | Q236H | Q245R | ||||
| V68A | N76D | S103A | V104l | N116T | G159D | R170S | N185S | A232V | Q236H | Q245R | ||
| G61E | V68A | S103A | V104l | G159D | A232V | Q236H | Q245R | N248D | N252K | |||
| N43D | V68A | S103A | V104l | G159D | A232V | Q236H | Q245R | N248D | N252K | |||
| V68A | S103A | V104l | G159D | S212P | A232V | Q236H | Q245R | N248D | N252K | |||
| V68A | S103A | V104l | S99N | G159D | N184D | A232V | Q236H | Q245R | N248D | N252K | ||
| S103A | V104l | G159D | A232Y | Q236H | Q245R | N248D | N252K | |||||
| V68A | S103A | V104l | G159D | Y209W | A232V | Q236H | Q245R | N248D | N252K | |||
| V68A | S103A | V104l | 0109R | G159D | A232V | Q236H | Q245R | N248D | N252K | |||
| G20R | V68A | S103A | V104l | G159D | A232V | Q236H | Q245R | N248D | N252K | |||
| V68A | S103A | V104l | G159D | Y209F | A232V | Q236H | Q245R | N248D | N252K | |||
| V68A | S103A | V104l | G159D | A232V | Q236H | Q245R | N248D | N252K | N261 | |||
| V68A | S103A | V104l | G159D | N185D | A232V | Q236H | Q245R | N248D | N252K | |||
| V68A | S103A | V104l | G159D | P210R | A232V | Q236H | Q245R | N248D | N252K | |||
| V68A | S103A | V104l | G159D | P210T | A232V | Q236H | Q245R | N248D | N252K | |||
| V68A | S103A | V104l | G159D | P210S | A232V | Q236H | Q245R | N248D | N252K | |||
| V68A | S103A | V104l | G159D | N185D | P210L | A232V | Q236H | Q245R | N248D | N252K | ||
| V68A | S103A | V104l | G159D | P210L | A232V | Q236H | Q245R | N248D | N252K | |||
| V68A | S103A | V104l | G159D | S212A | A232V | Q236H | Q245R | N248D | N252K | |||
| V68A | S103A | V104l | G159D | S212G | A232V | Q236H | Q245R | N248D | N252K | |||
| V68A | S103A | V104l | G159D | S212E | A232V | Q236H | Q245R | N248D | N252K | |||
| V68A | S103A | V104l | G159D | T213E | A232V | Q236H | Q245R | N248D | N252K | |||
| V68A | S103A | V104l | T213S | A232V | G236H | Q245R | N248D | N252K | ||||
| V68A | A103V | V104l | G159D | T213E | A232V | Q236H | Q245R | N248D | N252K |
PL 191 529 B1 ciąg dalszy tabeli 2
| V68A | S103A | V104l | G159D | T213R | A232V | Q236H | Q245R | N248D | N252K | |||
| V68A | S103A | V104l | G159D | T213G | A232V | Q236H | Q245R | N248D | N252K | |||
| V68A | S103A | V104l | G159D | A215Y | A232V | Q236H | Q245R | N248D | N252K | |||
| V68A | S103A | V104l | G159D | A215R | A232V | Q236H | Q245R | N248D | N252K | |||
| V68A | S103A | V104l | G159D | S216T | A232V | Q236H | Q245R | N248D | N252K | |||
| V68A | S103A | V104l | G159D | S216Y | A232V | Q236H | Q245R | N248D | N252K | |||
| V68A | S103A | V104l | G159D | S216C | A232V | Q236H | Q245R | N248D | N252K | |||
| G20A | V68A | S103A | V104ł | G159D | A232V | Q236H | Q245R | N248D | N252K | |||
| V68A | S103A | V104l | G159D | N173D | A232V | Q236K | Q245R | N248D | N252K | |||
| V68A | S103A | V104l | G159D | A232V | Q236H | Q245R | N248D | K251Y | N252K | |||
| V68A | S103A | V104l | G159D | Q206R | A232V | Q236H | Q245R | N248D | N252K | |||
| V68A | S103A | V104l | G159D | A232V | Q236H | Q245R | N248D | N252F | ||||
| V68A | S103A | V104l | G159D | A232V | Q236H | Q245R | N248D | N252L | ||||
| P55S | V68A | S103A | V104l | G159D | A232V | Q236H | Q245R | N248D | N252F | |||
| V68A | S103A | V104l | G159D | A232V | Q236H | Q245R | N248D | N252K | T255Y | |||
| V68A | S103A | V104l | G159D | A232V | Q236H | Q245R | N248D | N252K | S256N | |||
| V68A | S103A | V104l | G159D | A232V | Q236H | Q245R | N248D | N252K | S256E | |||
| V68A | S103A | V104l | G159D | A232V | Q236H | Q245R | N248D | N252K | S256R | |||
| V68A | S103A | V104l | G159D | A232V | Q236H | Q245R | N248D | N252K | T260R | |||
| V68A | S103A | V104l | G159D | A232V | Q236H | Q245R | N248D | N252K | L257R | |||
| V68A | S103A | V104l | G159D | A232V | Q236H | Q245R | N248D | N252K | G258D | |||
| 18V | V68A | S103A | V104l | G159D | A232V | Q236H | Q245R | N248D | N252K | N269D | ||
| V68A | S103A | V104l | N116S | G159D | A232V | Q236H | Q245R | N248D | N252K | T260E | ||
| V68A | S103A | V104l | G159D | A232V | Q236H | Q245R | N248D | N252K | N261R | |||
| V68A | S103A | V104l | G159D | A232V | Q236H | Q245R | N248D | N252K | N261D | |||
| V68A | N76D | S103A | V104l | G159D | A232V | Q236H | Q245R | N248D | N252K | |||
| V68A | S103A | V104l | A232V | Q236H | Q245R | N248D | N252K | |||||
| S103A | V104l | G159D | A232S | Q236H | Q245R | N248D | N252K | |||||
| V68A | S103A | V104l | G159D | A232Y | Q236R | Q245R | N248D | N252K | ||||
| N18S | V68A | S103A | V104l | G159D | A232V | Q236H | Q245R | N248D | N252K | |||
| V68A | S103A | V104l | G159D | A232Y | Q236H | Q245V | N248D | N252K | ||||
| V68A | N76D | S101T | S103A | V104l | G159D | T213R | N218S | A232V | Q236H | Q245R | T260A | |
| V68A | S103A | V104l | G159D | A228Y | A232V | Q236H | Q245R | N248D | N252K | |||
| T33S | V68A | N76D | S103A | V104l | G159D | A232V | Q236H | Q245R | N248D | N252K | ||
| V68A | N76D | E89D | S103A | V104l | G159D | P210L | T213R | A232V | Q236H | Q245R | T260A | |
| G61E | V68A | N76D | S103A | V104l | G159D | A232V | Q236H | Q245R | N248D | N252K | ||
| S103A | V104l | G159D | V205l | P210I | A232V | Q236H | Q245R |
PL 191 529 B1 ciąg dalszy tabeli 2
| G61E | V68A | S103A | V104l | S130A | G159D | A232V | Q236H | Q245R | N248D | N252K | ||
| G61E | V68A | S103A | V104l | A133S | Q137R | G159D | A232V | Q236H | Q245R | N248D | N252K | |
| G61E | S103A | V104l | A133Y | G159D | A232V | Q236H | Q245R | N248D | N252K | |||
| V68A | S103A | V104l | G159D | A232V | Q236H | Q245R | N248G | N262K | ||||
| V68A | S103A | V104l | G159D | N218S | A232V | Q236H | Q245R | N248D | N252K | |||
| G61E | V68A | S103A | V104l | G159D | S 160 | A232V | Q236H | Q245R | N248D | N252K | ||
| S3L | G61E | V68A | N76D | S103A | V104l | A232V | Q236H | Q245R | N248D | N252K | ||
| G61E | V68A | S103A | V104l | G159D | S167F | A232V | Q236H | Q245R | N248D | N252K | ||
| G97E | S103A | V104l | G159D | A232V | Q236H | Q245R | N248D | N252K | ||||
| A98D | S103A | V104l | G159D | A232V | Q236H | Q245R | N248D | N252K | ||||
| S99E | S103A | V104l | G159D | A232V | Q236H | Q245R | N248D | N252K | ||||
| S101E | S103A | V104l | G159D | A232V | Q236H | Q245R | N248D | N252K | ||||
| S101G | S103A | V104l | G159D | A232V | Q236H | Q245R | N248D | N252K | ||||
| G102A | S103A | V104l | G159D | A232V | Q236H | Q245R | N248D | N252K | ||||
| S103A | V104l | S106E | G159D | A232V | Q236K | Q245R | N248D | N252K | ||||
| S103A | V104l | Q109E | G159D | A232V | Q236K | Q245R | N248D | N252K | ||||
| S103A | V104l | G159D | A232V | Q236H | Q245R | N248D | N252K | N261 | ||||
| S103A | V104l | Q109R | G159D | A232V | Q236H | Q245R | N248D | N252K | ||||
| N62D | S103A | V104l | G159D | A232V | Q236H | Q245R | N248D | N252K | ||||
| S103A | V104l | G159D | N184D | A232V | Q236H | Q245R | N248D | N252K | ||||
| S103A | V104l | G159D | S166D | A232V | Q236H | Q245R | N248D | N252K | ||||
| S103A | V104l | G159D | L217E | A232V | Q236H | Q245R | N248D | N252K | ||||
| G20R | N62D | S103A | V104l | G159D | T213R | A232V | Q236H | Q245R | N248D | N252K | ||
| N62D | S103A | V104l | G159D | T213R | A232V | Q236H | Q245R | N248D | N252K | |||
| S103A | V104l | G159D | Q206R | L217E | A232V | Q236H | Q245R | N248D | N252K | |||
| N62D | S103A | V104l | G159D | Q206R | A232V | Q236H | Q245R | N248D | N252K | |||
| S103A | V104l | S130G | G159D | A232V | Q236H | Q245R | N248D | N252K | ||||
| S103A | V104l | P131Y | G159D | A232V | Q236H | Q245R | N248D | N252K | ||||
| K27N | S103A | V104l | G159D | A232V | Q236H | Q245R | N248D | N252K | ||||
| T38G | S103A | V104l | G159D | A232V | Q236H | Q245R | N248D | N252K | ||||
| T38A | N76D | S103A | V104l | G159D | T213R | A232V | Q236H | Q245R | T260A | |||
| V68A | N76D | S103A | V104l | G159D | T213R | A232V | Q236H | Q245R | T260A | E271G | ||
| V68A | N76D | S103A | V104l | G159D | Y209W | T213R | A232V | Q236H | Q245R | T260A | ||
| V68A | N76D | S103A | V104l | G159D | P210I | T213R | A232V | Q236H | Q245R | T260A | ||
| V68A | N76D | S103A | V104l | G159D | Y205I | T213R | A232V | Q236H | Q245R | T260A | ||
| V68A | N76D | S103A | V104l | G159D | P210I | A232V | Q236H | Q245R | T260A | |||
| V68A | S103A | V104l | G159D | T213R | A232V | Q236H | Q245R | T260A |
PL 191 529 B1 ciąg dalszy tabeli 2
| N76D | S103A | V104l | G159D | T213R | A232V | Q236H | Q245R | T260A | ||||
| V68A | S103A | V104l | G159D | Y209W | A232V | Q236H | Q245R | |||||
| V68A | S103A | V104l | G159D | P210I | A232V | Q236H | Q245R | |||||
| V68A | S103A | V104l | G159D | A230Y | A232V | Q236H | Q245R | |||||
| V68A | S103A | V104l | G159D | L126F | A232V | Q236H | Q245R | |||||
| V68A | S103A | V104l | G159D | Y205! | A232V | Q236H | Q245R | |||||
| V68A | S103A | V104l | G159D | P210L | A232V | Q236H | Q245R | |||||
| S103A | V104l | G159D | A230Y | Q236H | Q245R | |||||||
| V68A | S103A | V104l | G159D | A232V | Q236H | Q245R | T260A | |||||
| S103A | V104l | G159D | A232V | Q236H | Q245R | |||||||
| V68A | S103A | V104l | G159D | A174V | A232V | Q236H | Q245R | L257V | ||||
| V68A | S103A | V1041 | G159D | A194S | A232V | Q236H | Q245R | L257V | ||||
| V68A | S103A | V1041 | G159D | Y209W | A232V | Q236H | Q245R | L257V | ||||
| S103A | V1041 | G159D | A232Y | Q236H | Q245R | L257V | ||||||
| V68A | N76D | S103A | V1041 | G159D | T213R | A232V | Q236H | Q245R | T260A | N261W | ||
| V68A | S103A | V1041 | G159D | A232V | Q236H | Q245R | L257V | N261W | ||||
| S103A | V1041 | G159D | T213R | A232V | Q236H | Q245R | T260A | |||||
| S103A | V1041 | G159D | P210I | A232V | Q236H | Q245R | N248D | N252K | ||||
| S103A | Y104I | G159D | Y209W | A232V | Q236H | Q245R | L257V | |||||
| V68A | N76D | S103A | V104l | G159D | P210L | T213R | A232V | Q236H | Q245R | T260A | ||
| Q12R | S103A | V1041 | G159D | Y209W | T213R | A232V | Q236H | Q245R | T260A | |||
| S103A | V1041 | Y209W | A232V | Q236H | Q245R | L257V | * | |||||
| S103A | V1041 | G159D | V205l | P210I | T213R | A232V | Q236H | Q245R | T260A | |||
| S103A | Y104I | G159D | V205l | Y209W | A232V | Q236H | Q245R | T260A | ||||
| V68A | S103A | V1041 | G159D | V205l | Y209W | P210I | A232V | Q236H | Q245R | |||
| S103A | V1041 | G159D | V205l | Y209W | P210I | A232V | Q236H | Q245R | L257V | |||
| S103A | V1041 | G159D | V205l | Y209W | A232V | Q236H | Q245R | L257V | ||||
| V68A | S103A | V1041 | G159D | V205l | Y209W | P210I | A232V | Q236H | Q245R | T260A | ||
| S103A | V1041 | G159D | V205l | Y209W | P210I | A232V | Q236H | Q245R | ||||
| S103A | V1041 | G159D | Y209W | P210I | A232V | Q236H | Q245R | |||||
| S103A | V104l | G159D | V205l | P210I | A232V | Q236H | Q245R | |||||
| V68A | S103A | V104l | S128L | G159D | A232V | Q236H | Q245R | |||||
| A48V | S103A | V104l | G159D | A230Y | Q236H | Q245R | ||||||
| A48V | V68A | S103A | V104l | G159D | Y209W | A232V | Q236H | Q245R | ||||
| A48V | V68A | S103A | V104l | G159D | A232V | Q236H | Q245R | N248D | N252K | |||
| A48V | V68A | S103A | V104l | G159D | A232V | Q236H | Q245R | L257Y | N261W | |||
| G102A | S103A | V104l | G159D | S212G | A232V | Q236H | Q245R | N248D | N252K |
PL 191 529 B1 ciąg dalszy tabeli 2
| Q12R | G102A | S103A | V104l | G159D | S212G | A232V | Q236H | Q245R | N248D | N252K | ||
| S101G | G102A | S103A | V104l | G159D | S212G | A232V | Q236H | Q245R | N248D | N252K | ||
| A98L | G102A | S103A | V104l | G159D | S212G | A232V | Q236H | Q245R | N248D | N252K | ||
| G102A | S103A | V104l | G159D | T213R | A232V | Q236H | Q245R | N248D | N252K | |||
| S103A | Y104I | P131Y | G159D | A232V | Q236H | Q245R | N248D | N252K | ||||
| S103A | Y104I | G159D | N184S | A232V | Q236H | Q245R | N248D | N252K | ||||
| S103A | Y104I | G159D | N184G | A232V | Q236H | Q245R | N248D | N252K | ||||
| S103A | V104l | G159D | A232Y | Q236H | V244T | Q245R | N248D | N252K | ||||
| S103A | V104l | G159D | A232V | Q236H | Y244A | Q245R | N248D | N252K | ||||
| N62D | S103A | V104l | G159D | T213R | A232V | Q236H | Q245R | N248D | N252K | S256R | ||
| Q12R | N62D | S103A | V104l | G159D | T213R | A232V | Q236H | Q245R | N248D | N252K | ||
| S101G | S103A | V104l | G159D | N185D | A232V | Q236H | Q245R | N248D | N252K | |||
| S101G | S103A | V104l | G159D | Q206E | A232V | Q236H | Q245R | N248D | N252K | |||
| S101G | S103A | V104l | G159D | T213Q | A232V | Q236H | Q245R | N248D | N252K | |||
| A98L | G102A | S103A | V104l | G159D | A232V | Q236H | Q245R | N248D | N252K | |||
| S101G | G102A | S103A | V104t | G159D | A232V | Q236H | Q245R | N248D | N252K | |||
| A98L | G102A | S103A | V104l | G159D | S212G | A232V | Q236H | Q245R | N248D | N252K | ||
| A98L | G102A | S103A | V104l | G159D | S212G | A232V | Q236H | N248D | N252K | |||
| N62D | S103A | V104l | Q109R | G159D | T213R | A232V | Q236H | Q245R | N248D | N252K | ||
| N62D | S103A | V104l | G159D | S212G | T213R | A232V | Q236H | Q245R | N248D | N252K | ||
| N62D | S101G | S103A | V104l | G159D | S212G | T213R | A232V | Q236H | Q245R | N248D | N252K | |
| S103A | V104l | G159D | A232Y | Q245R | N248D | N252K | ||||||
| S103A | V104l | G159D | A230V | Q245R | ||||||||
| N62D | S103A | V104l | S130G | G159D | T213R | A232V | Q236H | Q245R | N248D | N252K | ||
| S101G | S103A | V104l | S130G | G159D | A232V | Q236H | Q245R | N248D | N252K | |||
| S101G | S103A | V104l | S128G | G159D | A232V | Q236H | Q245R | N248D | N252K | |||
| S101G | S103A | V104l | S128L | G159D | A232V | Q236H | Q245R | N248D | N252K | |||
| N62D | S101G | S103A | V104l | G159D | T213R | A232V | Q236H | Q245R | N248D | N252K | ||
| N62D | S103A | V104l | S128G | G159O | T213R | A232V | Q236H | Q245R | N248D | N252K | ||
| N62D | S103A | V104l | S128L | G159D | T213R | A232V | Q236H | Q245R | N248D | N252K | ||
| S101G | S103A | V104l | G159D | A232V | Q236H | Q245R | N248D | N252K | T260A | |||
| S101G | S103A | V104l | P131Y | G159D | A232V | Q236H | Q245R | N248D | N252K | |||
| A98V | S101G | S103A | V104l | G159D | A232V | Q236H | Q245R | N248D | N252K | |||
| S99G | S101G | S103A | V104l | G159D | A232V | Q236H | Q245R | N248D | N252K | |||
| S101G | S103A | V104l | G159D | S212G | A232V | Q236H | Q245R | N248D | N252K | |||
| S101G | S103A | Y104I | G159D | Y209W | A232V | Q236H | Q245R | N248D | N252K | |||
| S101G | S103A | Y104I | G159D | P210I | A232V | Q236H | Q245R | N248D | N252K |
PL 191 529 B1 ciąg dalszy tabeli 2
| S101G | S103A | V104l | G159D | Y205I | A232V | Q236H | Q245R | N248D | N252K | |||
| S101G | S103A | V104l | G159D | A230Y | 0236H | Q245R | ||||||
| S101G | S103A | V104l | G159 | A194P | A232V | Q236H | Q245R | N248D | N252K | |||
| N76D | S101G | S103A | V104l | G159D | A194P | A232V | Q236H | Q245R | N248D | N252K | ||
| S101G | S103A | V104l | G159D | A230Y | A232V | Q236H | Q245R | N248D | N252K | |||
| N62D | S103A | V104l | G159D | N185D | 0206E | T213R | A232V | Q236H | Q245R | N248D | N252K | E271Q |
Jeszcze bardziej korzystne odmiany proteazy zawierają zestaw podstawień wybrany z grupy obejmującej pozycje reszt odpowiadających pozycjom subtylizyny z Bacillus amyloliquefaciens przedstawionym w tabeli 3.
T ab el a 3
| 76 | 103 | 104 | 222 | 245 | ||||||||
| 76 | 103 | 104 | 222 | 249 | ||||||||
| 68 | 103 | 104 | 159 | 232 | 236 | 245 | 252 | |||||
| 68 | 76 | 103 | 104 | 159 | 213 | 232 | 236 | 245 | 260 | |||
| 22 | 68 | 76 | 103 | 104 | ||||||||
| 68 | 103 | 104 | 159 | 232 | 236 | 245 | 248 | 252 | ||||
| 68 | 103 | 104 | 159 | 232 | 236 | 245 | ||||||
| 68 | 103 | 104 | 140 | 159 | 232 | 236 | 245 | 252 | ||||
| 43 | 68 | 103 | 104 | 159 | 232 | 236 | 245 | 252 | ||||
| 43 | 68 | 103 | 104 | 159 | 232 | 236 | 245 | |||||
| 12 | 76 | 103 | 104 | 130 | 222 | 245 | 261 | |||||
| 76 | 103 | 104 | 130 | 222 | 245 | |||||||
| 68 | 103 | 104 | 159 | 232 | 236 | 245 | 257 | |||||
| 68 | 76 | 103 | 104 | 159 | 210 | 232 | 236 | 245 | ||||
| 68 | 103 | 104 | 159 | 224 | 232 | 236 | 245 | 257 | ||||
| 76 | 103 | 104 | 159 | 232 | 236 | 245 | 257 | |||||
| 68 | 76 | 103 | 104 | 159 | 211 | 232 | 236 | 245 | ||||
| 12 | 68 | 76 | 103 | 104 | 159 | 214 | 232 | 236 | 245 | |||
| 68 | 76 | 103 | 104 | 159 | 215 | 232 | 236 | 245 | ||||
| 12 | 68 | 76 | 103 | 104 | 159 | 232 | 236 | 245 | ||||
| 20 | 68 | 76 | 103 | 104 | 159 | 232 | 236 | 245 | 259 | |||
| 68 | 76 | 87 | 103 | 104 | 159 | 232 | 236 | 245 | 260 |
PL 191 529 B1 ciąg dalszy tabeli 3
| 68 | 76 | 103 | 104 | 159 | 232 | 236 | 245 | 261 | ||||
| 12 | 48 | 68 | 76 | 103 | 104 | 159 | 232 | 236 | 245 | |||
| 76 | 103 | 104 | 159 | 192 | 232 | 236 | 245 | |||||
| 76 | 103 | 104 | 147 | 159 | 232 | 236 | 245 | 248 | 251 | |||
| 12 | 68 | 76 | 103 | 104 | 159 | 232 | 236 | 245 | 272 | |||
| 68 | 76 | 103 | 104 | 159 | 183 | 206 | 232 | 236 | 245 | |||
| 68 | 76 | 103 | 104 | 159 | 232 | 236 | 245 | 256 | ||||
| 68 | 76 | 103 | 104 | 159 | 206 | 232 | 236 | 245 | ||||
| 27 | 68 | 76 | 103 | 104 | 159 | 232 | 236 | 245 | ||||
| 68 | 103 | 104 | 159 | 212 | 232 | 236 | 245 | 248 | 252 | |||
| 103 | 104 | 159 | 232 | 236 | 245 | 248 | 252 | |||||
| 68 | 103 | 104 | 159 | 209 | 232 | 236 | 245 | 248 | 252 | |||
| 68 | 103 | 104 | 109 | 159 | 232 | 236 | 245 | 248 | 252 | |||
| 20 | 68 | 103 | 104 | 159 | 232 | 236 | 245 | 248 | 252 | |||
| 68 | 103 | 104 | 159 | 209 | 232 | 236 | 245 | 248 | 252 | |||
| 68 | 103 | 104 | 159 | 210 | 232 | 236 | 245 | 248 | 252 | |||
| 68 | 103 | 104 | 159 | 212 | 232 | 236 | 245 | 248 | 252 | |||
| 68 | 103 | 104 | 159 | 213 | 232 | 236 | 245 | 248 | 252 | |||
| 68 | 103 | 104 | 213 | 232 | 236 | 245 | 248 | 252 | ||||
| 68 | 103 | 104 | 159 | 215 | 232 | 236 | 245 | 248 | 252 | |||
| 68 | 103 | 104 | 159 | 216 | 232 | 236 | 245 | 248 | 252 | |||
| 20 | 68 | 103 | 104 | 159 | 232 | 236 | 245 | 248 | 252 | |||
| 68 | 103 | 104 | 159 | 232 | 236 | 245 | 248 | 252 | 255 | |||
| 68 | 103 | 104 | 159 | 232 | 236 | 245 | 248 | 252 | 256 | |||
| 68 | 103 | 104 | 159 | 232 | 236 | 245 | 248 | 252 | 260 | |||
| 68 | 103 | 104 | 159 | 228 | 232 | 236 | 245 | 248 | 252 | |||
| 68 | 76 | 89 | 103 | 104 | 159 | 210 | 213 | 232 | 236 | 245 | 260 | |
| 68 | 103 | 104 | 159 | 218 | 232 | 236 | 245 | 248 | 252 |
Wynalazek jest dodatkowo objaśniony na rysunku, na którym: fig. 1 przedstawia sekwencje DNA i aminokwasową subtylizyny z Bacillus amyloliquefaciens oraz częściową mapę restrykcyjną tego genu; fig. 2 przedstawia konserwowane reszty aminokwasowe subtylizyn z Bacillus amyloliquefaciens (BPN1) i Bacillus lentus (naturalna); oraz fig. 3 A i 3 B przedstawiają sekwencje aminokwasowe czterech subtylizyn w następującym porządku: pierwsza linia dotyczy sekwencji aminokwasowej subtylizyny z Bacillus amyloliquefaciens (zwanej także subtylizyną BPN'), druga linia - z Bacillus subtylis, trzecia - z Bacillus licheniformis, a czwarta - Bacillus lentus (zwanej także subtylizyną 309 w opisie PCT W089/06276). Symbol * oznacza brak specyficznych reszt aminokwasowych w porównaniu do subtylizyny BPN'.
PL 191 529 B1
W niniejszym opisie określenie „proteza” oznacza proteazę naturalną lub rekombinowaną, gdzie proteazy są hydrolazami karbonylowymi, których działanie polega ogólnie na cięciu wiązań białek lub peptydów. Do występujących naturalnie proteaz należą α-aminoacylopeptydohydrolaza, peptydyloaminohydrolaza kwasowa, acyloaminohydrolaza, karboksypeptydazaserynowa, metalokarboksypeptydaza, proteinaza tiolowa, karboksyloproteinaza oraz metaloproteinaza. Należą tu także proteazy serynowe, metalo-, tiolo-oraz kwasowe, a także endo -i egzoproteazy.
Niniejszy wynalazek dotyczy enzymów proteazowych, które nie stanowią odmian naturalnie występującej hydrolazy karbonylowej. Mają one odmienną czynność proteolityczną, stabilność, specyficzność dla substratów, profil pH i/lub wydajność w porównaniu do wyjściowej hydrolazy karbonylowej, z której pochodzi sekwencja aminokwasowa odmiany. Takie odmiany proteazy zawierają sekwencje aminokwasowe nieznane w naturze, a powstałe w wyniku podstawienia wielu reszt aminokwasowych proteazy wyjściowej przez różne aminokwasy. Proteazy wyjściowe mogą być naturalne lub rekombinowane.
Użyteczne odmiany proteaz według wynalazku zawierają podstawienia określonego z dziewiętnastu naturalnie występujących L-aminokwasów w określonych położeniach reszt aminokwasowych. Takich podstawień można dokonać w dowolnej wyjściowej subtylizynie (prokariotycznej, eukariotycznej, pochodzącej od ssaków itd.). W niniejszym opisie aminokwasy określa się za pomocą standardowego kodu jedno- lub trzyliterowego.
W niniejszym opisie określenie „subtylizyna”oznacza subtylizynę naturalną lub rekombinowaną, gdzie subtylizyny są bakteryjnymi lub grzybowymi proteazami, których działanie polega na cięciu wiązań peptydowych w białkach lub peptydach. Różne gatunki drobnoustrojów wytwarzają i wydzielają wiele naturalnych subtylizyn. Sekwencje aminokwasowe tych subtylizyn nie są całkowicie homologiczne, jednakże wykazują one ten sam lub podobny typ aktywności proteolitycznej. Ta klasa proteaz serynowych ma wspólną sekwencjęaminokwasową z triadą katalityczną, która odróżnia je od proteaz pokrewnych chymotrypsynie. Wprawdzie w obu tych grupach proteaz występuje triada złożona z asparaginianu, histydyny i seryny, ale położenie tych reszt jest różne. Odczytując od końca N wkierunku końca C, kolejność dla proteaz spokrewnionych z subtylizyną jest następująca: asparaginian - histydyna - seryna, podczas gdy w przypadku spokrewnionych z chymotrypsyną: histydyna asparaginian - seryna. A zatem subtylizyny według wynalazku są proteazami serynowymi z triadą katalityczną charakterystyczną dla proteaz spokrewnionych z subtylizyną. Przykłady przedstawiono na fig. 3. Wopisie niniejszego wynalazku numeracja aminokwasów w proteazach odpowiada numerom podanym dla sekwencji subtylizyny z dojrzałej Bacillus amyloliquefaciens, przedstawionej na fig. 1.
„Rekombinacyjna subtylizyna” lub „rekombinacyjna proteza” oznacza subtylizynę lub proteazę, w której sekwencja DNA kodująca subtylizynę lub proteazę jest zmodyfikowana tak, aby wytworzyć odmienną (lub zmutowaną) sekwencję DNA, która koduje podstawienie, delecję lub insercję przynajmniej jednego aminokwasu w naturalnie występującej sekwencji aminokwasowej.
Odpowiednie sposoby wytwarzania takich modyfikacji są dostępne w literaturze.
„Subtylizyny nie pochodzące od człowieka” i kodujące je DNA można uzyskać z wielu organizmów prokariotycznych i eukariotycznych. Przykładami organizmów prokariotycznych są bakterie Gram ujemne, takie jak E. coli lub Pseudomonas oraz bakterie Gram dodatnie, jak Micrococcus lub Bacillus; eukariotyczne -drożdże,np. Saccharomyces cerevisiae oraz grzyby, takie jak Aspergillus sp.
„Odmiana proteazy” ma sekwencję aminokwasową pochodzącą z „proteazy wyjściowej”. Doproteaz wyjściowych należą proteazy naturalne i rekombinowane. Sekwencja aminokwasowa odmiany proteazy jest pochodną sekwencji proteazy wyjściowej uzyskaną przez podstawienie, delecje lub insercje przynajmniej jednego aminokwasu w sekwencji wyjściowej. Taka modyfikacja dotyczy „wyjściowej sekwencji DNA”, kodującej sekwencję aminokwasową proteazy wyjściowej niż samego enzymu jako takiego. Odpowiednie sposoby takiej manipulacji wyjściową sekwencją DNA podaje niniejszy opis,jak również znane są z literatury.
Numeracja aminokwasów odpowiada numerom podanym dla sekwencji subtylizyny z dojrzałego Bacillus amyloliquefaciens, przedstawionej na fig. 1. Wynalazek nie ogranicza się jednakże do mutacji tej wybranej subtylizyny, ale dotyczy również proteaz wyjściowych zawierających reszty aminokwasowe w położeniach „ekwiwalentnych” ze zidentyfikowanymi dla subtylizyny z Bacillus amyloliquefaciens. Pozycję uważa się za ekwiwalentną do pozycji z subtylizyny Bacillus amyloliquefaciens w przypadku, jeżeli jest ona albo homologiczna (tj. odpowiada jej na poziomie zasadniczej lub trzeciorzędowej struktury) albo analogiczna do specyficznej reszty bądź jej części z subtylizyny Bacillus amyloliquefaciens (tj. ma taką samą zdolność do wiązania się, reakcji lub interakcjichemicznej).
PL 191 529 B1
W celu ustalenia homologiczności struktury zasadniczej, sekwencję proteazy wyjściowej porównuje się bezpośrednio z zasadniczą sekwencją subtylizyny z Bacillus amyloliquefaciens, a zwłaszcza z zestawem reszt znanych jako niezmienne dla subtylizyn o znanej sekwencji. Przykładowo, na fig. 2 przedstawiono zachowane reszty subtylizyn z B. amyloliquefaciens i B. lentus. Po ich przeprowadzeniu w postać liniową, z uwzględnieniem koniecznych insercji i delecji, aby zachować liniowość (tj. uniknąć eliminacji reszt przez arbitralne delecje i insercje), definiuje się reszty równoważne do poszczególnych aminokwasów w pierwszorzędowej sekwencji subtylizyny z Bacillus amyloliquefaciens.
Zgodność zachowanych reszt korzystnie powinna dotyczyć 100% takich reszt, jednakże zgodność obejmująca więcej niż 75% bądź około 50% jest wystarczająca do zdefiniowania równoważnych pozostałości. Powinna być zachowana triada katalityczna, Asp32 / His64 / Ser221. Wskazano zgodność znacznej ilości proteaz serynowych, grupując je jako subtylazy i subtylizynopodobne.
Przykładowo, na fig. 3 przedstawiono w postaci liniowej sekwencje aminokwasowe z Bacillus amyloliquefaciens, Bacillus subtylis, Bacillus licheniformis (carlsbergensis) i Bacillus lentus, celem wykazania maksymalnej homologiczności tych sekwencji. Porównanie wskazuje, że w każdej sekwencji obecna jest pewna ilość konserwowanych reszt. Reszty te zidentyfikowano na fig. 2 (dla BPN'i B. lentus).
Te konserwowane reszty mogą być użyte do identyfikacji odpowiednich równoważnych reszt aminokwasowych subtylizyny z Bacillus amyloliquefaciens w innych subtylizynach, jak subtylizyna z Bacillus lentus (publikacja PCT nr W089/06279 z 13 lipca 1989), która jest korzystną proteazą wyjściową lub subtylizyna znana jako PB92 (opis patentowy europejski EP 0 328 299), która wykazuje znaczna homologiczność z wyżej wymienioną. Sekwencje aminokwasowe niektórych subtylizyn przedstawiono w postaci liniowej na fig. 3A i 3B w porównaniu z sekwencjami subtylizyny z Bacillus amyloliquefaciens dla wykazania zgodności konserwowanych reszt. Jak widać, w sekwencji Bacillus lentus występuje pewna ilość delecji w porównaniu z subtylizyną z Bacillus amyloliquefaciens. Przykładowo, ekwiwalentnym aminokwasem dla Val165 w subtylizynie z Bacillus amyloliquefaciens jest izoleucyna w subtylizynach z B. lentus i B. licheniformis.
„Reszty ekwiwalentne” można także zdefiniować przez określenie homologiczności na poziomie struktury trzeciorzędowej proteazy wyjściowej, którą określa się metodą krystalografii rentgenowskiej. Jako reszty ekwiwalentne uważa się takie reszty, w których współrzędne atomowe przynajmniej dwóch atomów z głównego łańcucha danej reszty aminokwasowej proteazy wyjściowej i subtylizyny z Bacillus amyloliquefaciens ( N na N, CAna CA, C na C i O na O) znajdują się w zakresie 0,13 nm, a korzystnie 0,1 nm po przeprowadzeniu w postać liniową. Zgodność uzyskuje się po zorientowaniu i ustawieniu najlepszego modelu tak, aby uzyskać maksymalne nałożenie współrzędnych atomowych dla atomów (nie będących atomami wodoru) cząsteczki danej proteazy i subtylizyny z Bacillus amyloliquefaciens. Najlepszym modelem jest taki model krystalograficzny, dla którego uzyskuje się najniższą wartość współczynnika R dla danych krystalograficznych przy najwyższej osiągalnej rozdzielczości.
ιη, ·, ·, η Eh |Fo(h)-Fc(h)|
Współczynnik R = h——-L21
Ph | Fo(h) |
Resztami ekwiwalentnymi, które są funkcjonalnie analogiczne do danej reszty subtylizyny z Bacillus amyloliquefaciens, określa się te aminokwasy proteazy wyjściowej, które mogą przyjąć konformację prowadzącą do zmiany, modyfikacji lub udziału w budowie białka, wiązaniu substratów lub katalizie w sposób zdefiniowany i przypisany danej reszcie subtylizyny z Bacillus amyloliquefaciens. Ponadto są to takie reszty z proteazy wyjściowej (o strukturze trzeciorzędowej określonej metodą krystalografii rentgenowskiej), które zajmują analogiczne pozycje w takim stopniu, że chociaż atomy głównego łańcucha nie spełniają kryterium ekwiwalentności na podstawie homologiczności położenia, to współrzędne atomowe przynajmniej dwóch atomów w łańcuchu bocznym leżą w zakresie 0,13 nm od odpowiednich atomów w łańcuchu bocznym subtylizyny z Bacillus amyloliquefaciens. Współrzędne trójwymiarowej struktury subtylizyny z Bacillus amyloliquefaciens podano w publikacji EPO nr 0 251 446 i można ich używać do wyznaczania reszt równoważnych, jak wyżej podano.
Niektóre z reszt, wykorzystywanych do podstawień, są resztami konserwowanymi, podczas gdy inne - nie. Jeżeli chodzi o reszty niekonserwowane, podstawienia przynajmniej jednego aminokwasu są ograniczone do takich, w których powstają odmiany z sekwencjami aminokwasowymi innymi od istniejących w naturze. W przypadku reszt konserwowanych podstawienia nie powinny prowadzić do powstania sekwencji naturalnych. Odmiany proteazy według wynalazku są odmianami dojrzałymi, jak
PL 191 529 B1 również formami pro- i prepro- tych odmian. Formy prepro- są najkorzystniejsze, gdyż ułatwia to ekspresję, wydzielanie i dojrzewanie odmian proteaz.
Określenie „prosekwencja” dotyczy sekwencji aminokwasowej dołączonej do końca N dojrzałej postaci proteazy, która usunięta, daje „dojrzałą” postać proteazy. Wiele enzymów proteolitycznych występuje w naturze jako produkty proenzymów translacyjnych i, przy braku obróbki potranslacyjnej, ulega ekspresji w taki sposób. Korzystną prosekwencją do wytwarzania odmian proteazy jest domniemana prosekwencją subtylizyny z Bacillus amyloliquefaciens, chociaż można używać także innych prosekwencji proteazowych.
Określenie „sekwencja sygnałowa” lub „presekwencja” odnosi się do każdej sekwencji aminokwasowej dołączonej do końca N proteazy lub proproteazy, która może wziąć udział w wydzielaniu dojrzałych proteaz lub ich proform. Jest to definicja funkcjonalna, oznaczająca włączenie wszystkich sekwencji aminokwasowych kodowanych przez gen kodujący koniec N proteazy, biorących udział wwydzielaniu proteazy w naturalnych warunkach. Niniejszy wynalazek wykorzystuje takie sekwencje, aby wywołać wydzielanie odpowiednich odmian proteaz. Jedna z możliwych sekwencji sygnałowych zawiera pierwszych siedem reszt aminokwasowych sekwencji sygnałowej subtylizyny z Bacillus subtylis połączonych z pozostałą częścią sekwencji sygnałowej subtylizyny z (ATCC21536).
Postać „prepro”odmiany proteazy składa się z dojrzałej postaci proteazy mającej Bacillus lentus presekwencję czynnie sprzężoną z końcem N prosekwencji.
„Wektor ekspresyjny” oznacza konstrukt DNA zawierający sekwencję DNA funkcjonalnie połączoną z odpowiednią sekwencją regulatorową zdolną do kontrolowania ekspresji wspomnianego DNA wkomórce wybranego gospodarza. Takie sekwencje regulatorowe zawierają promotor inicjujący transkrypcję, sekwencję operatora regulacji transkrypcji, sekwencję kodującą miejsce wiązania rybosomu iodpowiedniego mRNA oraz sekwencje regulujące terminację transkrypcji i translacji. Wektor może być plazmidem, cząstką faga lub ewentualnie insertem genomowym. Po transformacji odpowiednich komórek gospodarza, wektormoże ulec replikacji i działać niezależnie od genomu gospodarza, bądź w niektórych przypadkach, integrować się z genomem. W niniejszym opisie określenia „wektor” oraz „plazmid” używane są zamiennie, ponieważ obecnie plazmid jest najczęściej używaną formą wektora. Jednakże stosuje się także inne znane postacie wektorów ekspresyjnych, spełniające równoważne funkcje.
„Komórki gospodarza” według wynalazku mogą być prokariotyczne lub eukariotyczne. Poddaje się je manipulacjom tak, aby uczynić je niezdolnymi do wydzielania enzymatycznie aktywnych endoproteaz. Korzystnym gospodarzem do ekspresji proteaz jest szczep Bacillus BG2036, który ma deficyt enzymatycznie aktywnej obojętnej proteazy i proteazy alkalicznej (subtylizyny). Innymi stosowanymi gospodarzami są szczepy: Bacillus subtylis 1168 i inne wybrane z B. licheniformis, B. lentus, itd.
Komórki gospodarza są transformowane lub transfektowane wektorami otrzymanymi znanymi sposobami rekombinacji DNA. Po transformacji są one zdolne do replikacji wektorów kodujących odmiany proteazy lub ekspresji pożądanych odmian proteaz. W przypadku wektorów kodujących formy pre- lub prepro- odmian proteaz, takie odmiany są zazwyczaj wydzielane z komórki gospodarza do otoczenia tej komórki.
„Funkcjonalnie połączony”, odnośnie zależności dwóch obszarów DNA, oznacza po prostu ich funkcjonalną współzależność. Przykładowo, presekwencja jest funkcjonalnie połączona z peptydem, jeżeli działa jako sekwencja sygnałowa, uczestnicząca w wydzielaniu dojrzałej postaci białka najbardziej prawdopodobnie z odcięciem sekwencji sygnałowej. Promotor jest funkcjonalnie połączony zsekwencją kodującą, jeżeli reguluje jej transkrypcję; miejsce wiązania rybosomu jest funkcjonalnie połączone z sekwencją kodującą, jeżeli znajduje się w położeniu umożliwiającym translację.
Geny kodujące naturalnie występującą proteazę wyjściową można otrzymać ogólnie znanymi sposobami. Należą do nich: syntetyzowanie oznaczonych sond zawierających domniemane sekwencje kodujące obszarów pożądanych proteaz, przygotowanie bibliotek genomicznych z organizmów zekspresją proteazy oraz przeszukiwanie bibliotek celem odszukania pożądanego genu przez hybrydyzację z sondą. Pozytywnie zhybrydyzowane klony są następnie mapowane i sekwencjonowane. Sklonowaną proteazę stosuje się z kolei do transformowania komórek gospodarza w celu uzyskania ekspresji proteazy. Gen proteazy jest dołączany do plazmidu wielokopijnego. Taki plazmid replikuje się w organizmie gospodarza w tym sensie, że zawiera dobrze znane elementy niezbędne do replikacji plazmidu: promotor czynnie sprzężony z danym genem (który może być dostarczony jako jego własny, homologiczny promotor, jeżeli jest rozpoznany, tj. transkrybowany, przez gospodarza), obszar terminacji transkrypcji i poliadenylacji (niezbędny dla stabilności mRNA transkrybowanego przez gospodarza z genu proteazy w pewnych eukariotycznych komórkach gospodarzy), który jest egzogeniczny lub
PL 191 529 B1 dostarczony przez obszar endogenicznego terminatora genu proteazy oraz gen selekcyjny, taki jak gen oporności na antybiotyk, który umożliwia uzyskanie selekcji hodowli komórek gospodarza zainfekowanych plazmidem poprzez hodowlę w pożywce zawierającej antybiotyk. Plazmidy wielokopijne zawierają także początek replikacji odpowiedni dla gospodarza, umożliwiając wytwarzanie dużej ilości plazmidów w cytoplazmie bez ograniczeń chromosomowych.
Wynalazek obejmuje integrację wielu kopii genu proteazy z genomem gospodarza. Ułatwiają to organizmy prokariotyczne i eukariotyczne, które są szczególnie czułe na homologiczną rekombinację.
Gen może pochodzić z naturalnego B. lentus, ewentualnie wytwarza się syntetyczny gen kodujący naturalną lub zmutowaną proteazę wyjściową. W takim przypadku określa się sekwencję DNA i/lub aminokwasową proteazy wyjściowej. Następnie syntetyzuje się wielokrotne, nakładające się fragmenty DNA o pojedynczej nici, które poddaje się hybrydyzacji i łączy celem wytworzenia DNA kodującego wyjściową proteazę. Po sklonowaniu naturalnego lub syntetycznego genu proteazy wykonano szereg modyfikacji prowadzących do wzmocnienia zastosowania genu poza syntezą naturalnej proteazy wyjściowej. Te modyfikacje obejmują produkcję proteaz rekombinowanych, a także produkcję odmian proteaz według wynalazku.
W celu ułatwienia utworzenia odmiany proteazy według wynalazku można zastosować poniżej opisany sposób mutagenezy kasetowej, chociaż możliwe jest zastosowanie również innych sposobów. Najpierw uzyskuje się natywny gen kodujący proteazę, po czym poddaje się go sekwencjonowaniu w całości lub części. Następnie przeszukuje się sekwencję szukając miejsca, w którym zamierza się w kodowanym enzymie dokonać mutacji przynajmniej jednego aminokwasu (delecji, insercji lub podstawienia). W sekwencjach flankujących to miejsce sprawdza się obecność miejsc restrykcyjnych wcelu wymiany krótkiego fragmentu genu na inny oligonukleotyd, który po ekspresji może kodować różne mutanty. Korzystnie, aby ułatwić wymianę fragmentu genu, wykorzystuje się unikalne miejsca restrykcyjne w genie proteazy. Jednakże inne odpowiednie miejsce restrykcyjne, może być wykorzystane, które występuje w genie proteazy niezbyt licznie, pod warunkiem, że uzyskane fragmenty genów będą mogły być połączone w odpowiedniej kolejności. Jeżeli miejsca restrykcyjne nie występują w odpowiedniej odległości od wybranego punktu (od 10 do 15 nukleotydów), uzyskuje sieje przez podstawianie nukleotydów w genie w taki sposób, aby w ostatecznym fragmencie nie została zmieniona ani ramka odczytu, ani kodowane aminokwasy. Mutację w genie prowadzącą do zmiany jego sekwencji na pożądaną, wykonuje się znanymi sposobami przy użyciu startera M13. Lokalizację odpowiednich obszarów flankujących i ocenę niezbędnych zmian w sekwencjach dwóch odpowiednich pozycji restrykcyjnych wykonuje się rutynowo z uwzględnieniem zredukowanego kodu genetycznego, mapy enzymów restrykcyjnych i wielu różnych enzymów. Należy zauważyć, że jeżeli dostępne jest odpowiednie flankujące miejsce restrykcyjne, powyższy sposób może być użyty tylko w stosunku do obszaru flankującego, który nie zawiera tego miejsca.
Po sklonowaniu natywnego lub syntetycznego DNA, miejsca restrykcyjne flankujące sekwencje przeznaczone do mutacji tnie się odpowiednimi enzymami restrykcyjnymi i wiele kaset oligonukleotydowych komplementarnych do N-końca liguje się z genem. Taki sposób upraszcza mutagenezę, gdyż wszystkie oligonukleotydy można syntetyzować tak, aby miały te miejsca restrykcyjne i nie potrzeba żadnych syntetycznych linkerów do utworzenia tych miejsc.
W niniejszym opisie, aktywność proteolityczną definiuje się jako szybkość hydrolizy wiązań peptydowych na miligram aktywnego enzymu. Opracowano wiele sposobów jej mierzenia. Oprócz lub zamiast zmodyfikowanej aktywności proteolitycznej, odmiany enzymów według wynalazku mają inne zmodyfikowane właściwości, określonych wartościami Km, kcat, stosunek kcat/Km oraz/lub zmodyfikowaną specyficzność substratową, oraz/lub zmodyfikowany profil aktywności w zakresie pH. Enzymy można dopasowywać do poszczególnych substratów, których obecność jest antycypowana, na przykład w przygotowaniu peptydów lub do procesów hydrolitycznych, takich jak pranie.
Celem wynalazku jest uzyskanie odmiany proteazy o zmienionej w porównaniu z wyjściową proteazą, korzystnie lepszej wydajności prania, w przynajmniej jednej recepturze środka piorącego i przynajmniej jednych warunkach prania.
Warunki prania obejmują dobór środka piorącego, ilość wody, temperatury wody oraz czasu prania. W różnych obszarach używa się środków piorących w różnych stężeniach. Przykładowo zawartość środka piorącego w wodzie z płukania wynosi w Europie: 4500-5000 ppm, w Japonii: około 667 ppm, a w Ameryce Północnej, zwłaszcza Stanach Zjednoczonych: około 975 ppm.
Układ o niskiej zawartości środka piorącego zawiera ten składnik w ilości mniejszej od 800 ppm. Przykładem są tu japońskie środki piorące o zawartości około 667 ppm składnika środka piorącego.
PL 191 529 B1
W układach o średniej zawartości środka piorącego wynosi ona od 800 ppm do 2000 ppm. Jako przykłady można wymienić środki piorące z Ameryki Północnej zawierające około 975 ppm środka piorącego w kąpieli pralniczej, a także brazylijskie - z zawartością około 1500 ppm.
Układ o dużej zawartości środka piorącego obejmują środki piorące o zawartości składników piorących w ilości powyżej 2000 ppm w kąpieli pralniczej. Dotyczy to kąpieli pralniczych typowych dla Europy, zawierających od 4500 ppm do 5000 ppm środka piorącego.
W środkach piorących latynoamerykańskich występuje duża ilość fosforanowych polepszaczy pienistości, a ilość składnika piorącego wynosi od 1500 ppm do 6000 ppm, co pozwala zaliczyć je do układów o średnim, bądź wysokim stężeniu środków piorących.
Przedstawione przykłady wskazują, że w skali świata zawartość środków piorących w kąpielach pralniczych jest znacznie zróżnicowana. Stężenia określano doświadczalnie. Na przykład, w Stanach Zjednoczonych, w typowej maszynie mieści się 64,4 l kąpieli pralniczej, a zatem dla uzyskania stężenia około 975 ppm środka piorącego należy dodać około 62,79 g tego składnika. Jest to typowa ilość wprowadzana przez klienta korzystającego z miarki dołączanej do opakowania. Ponadto stosuje się także różne temperatury prania, przy czym przykładowo w Japonii są one niższe niż w Europie.
Na podstawie wyników przeszukiwania odmian proteaz uważa się, że mutacje obserwowane wsubtylizynie z Bacillus amyloliquefaciens są istotne dla czynności proteolitycznej, wydajności i/lub stabilności tych enzymów oraz ich przydatności do celów pralniczych.
Wiele odmian proteaz według wynalazku jest przydatnych jako składniki środków piorących lub środków higieny osobistej, jak szampony i płyny kosmetyczne. W ich skład wchodzą także odpowiednie środki powierzchniowo czynne, mające charakter niejonowy, anionowy, kationowy lub amfoteryczny. Odpowiedni skład środka piorącego przedstawia przykład 7 w opisie patentowym Stanów Zjednoczonych Ameryki Północnej nr 5204 015. Odmiany proteaz według wynalazku znajdują zastosowanie również do innych celów, jak mydła stałe lub płynne, płyny do mycia naczyń, płyny do mycia soczewek kontaktowych, hydroliza peptydów, oczyszczanie ścieków, włókiennictwo, produkcja białek itd. Zwiększona wydajność w składzie środka piorącego oznacza lepsze usuwanie plam z substancji podatnych nadziałanie enzymów, jak trawa lub krew. Proteazy według wynalazku można wprowadzać w ilości od 0,01% do 5% (korzystnie 0,1% do 0,5%) wagowych do proszkowych lub ciekłych środków piorących, mających pH od 6,5 do 12,0. Takie środki piorące mogą zawierać także inne enzymy, jak znane proteazy, amylazy, celulazy, lipazy lub endoglikozydazy oraz wypełniacze aktywne i stabilizatory.
Dodatek proteaz według wynalazku do zwykłych kompozycji czyszczących nie ogranicza ich zastosowania. Innymi słowy, każda temperatura i wartość pH odpowiednia dla danego środka piorącego, będzie także odpowiednia dla kompozycji z tymi proteazami, jeśli pH znajduje się w określonym zakresie, a temperatura nie przekracza wartości, przy której zachodzi denaturacja proteazy. Proteazy te mogą być także składnikami czyszczących kompozycji bezdetergentowych.
Wynalazek obejmuje w kompozycjach zawierających proteazy według wynalazku, także inne typowe składniki kompozycji do czyszczenia, jak wybielacze, środki powierzchniowo czynne, wypełniacze, enzymy i katalizatory bielenia. Przy tworzeniu składu należy brać pod uwagę wzajemną kompatybilność składników.
Ilość dodatków w kompozycji do czyszczenia wynosi od 0,01% do 99,9%, korzystnie od 1% do 95%, a zwłaszcza korzystnie od 1% do 80%.
Poniższych przykładów nie należy traktować jako ograniczenia wynalazku.
Przykład 1
Uzyskano dużą ilość odmian proteazy i oczyszczono je znanymi sposobami. Wszystkich mutacji dokonano w subtiiizynie GG36 z Bacillus lentus. Odmiany przedstawiono w tabeli 3, powyżej.
Przykład 2
Badano wydajność dużej ilości odmian proteaz uzyskanych w przykładzie 1, w dwóch rodzajach środków do prania i warunków prania, stosując sposób opisany w „An improved method of assaying for a preferred enzyme and/or preferred detergent composition”, U. S. Serial No. 60/068,796.
W tabeli 4 wymieniono badane odmiany proteaz i wyniki badań w dwóch różnych środkach piorących. Wyniki w kolumnie A dotyczą 0,67 g/l filtrowanego środka piorącego Ariel Ultra (Procter & Gamble, Cincinnati, OH, USA) w roztworze o mieszanej twardości wapniowo/magnezowej, w ilości 3 gramów na 4 litry oraz 0,3 ppm enzymu w każdej studzience w temperaturze 20°C; w kolumnie B, dane dotyczą 3,38 g/l filtrowanego środka piorącego Ariel Futur (Procter & Gamble, Cincinnati, OH, USA) w roztworze o mieszanej twardości wapniowe/ magnezowej w wysokości 15 gramów na 4 litry oraz 0.3 ppm enzymu w każdej studzience w temperaturze 40°C.
PL 191 529 B1
Tab el a 4
| A | B | |||||||||||
| N76D | S103A | Y104I | 1 | 1 | ||||||||
| S103A | V104l | A228T | 0.56 | 1.11 | ||||||||
| V68A | S103A | V104l | G159D | A232V | 0236H | Q245R | N252K | 1.41 | 1.85 | |||
| V68A | S103A | V104l | G159D | A232Y | Q236H | Q245R | N248D | N252K | 2.77 | 1.20 | ||
| V68A | S103A | V104l | G159D | A232V | Q236H | Q245R | 2.26 | 1.67 | ||||
| V68A | S103A | V104l | N140D | G159D | A232V | Q236H | Q245R | N252K | 2.96 | 1.42 | ||
| N43S | V68A | S103A | V104l | G159D | A232V | Q236H | Q245R | N252K | 1.91 | 1.80 | ||
| N43K | V68A | S103A | V1OAI | G159D | A232V | Q236H | Q245R | 2.05 | 1.78 | |||
| N43D | V68A | S103A | V104l | G159D | A232V | Q236H | Q245R | N252K | 2.00 | 1.34 | ||
| V68A | S103A | V104l | G159D | A232V | Q236H | Q245R | L257Y | 2.38 | 1.67 | |||
| V68A | S103A | V104l | G159D | A232V | Q236H | Q245R | N248D | 2.83 | 0.53 | |||
| V68A | S103A | V104l | G159D | A232V | Q236H | K237E | Q245R | 2.87 | 0.20 | |||
| V68A | S103A | V104l | G159D | A232V | Q236H | Q245R | N252S | 2.56 | 1.41 | |||
| V68A | S103A | V104l | G159D | A232V | Q236H | Q245R | L257Y | R275H | 3.97 | 0.47 | ||
| V68A | S103A | V104l | G159D | T224A | A232V | Q236H | Q245R | L257Y | 3.35 | 1.28 | ||
| G61E | V68A | S103A | V104l | G159D | A232V | Q236H | Q245R | N248D | N252K | 3.77 | 0.09 | |
| N43D | V68A | S103A | V104l | G159D | A232V | Q236H | Q245R | N248D | N252K | 3.50 | 0.47 | |
| V68A | S103A | V104l | G159D | S212P | A232V | Q236H | Q245R | N248D | N252K | 2.81 | 1.46 | |
| N76D | A98E | S103A | V104l | 1.56 | 0.28 | |||||||
| V4E | N76D | S103A | V104l | 1.22 | 0.33 | |||||||
| N76D | N77D | S103A | V104l | 1.13 | 0.36 | |||||||
| A16T | N76D | S103A | V104l | N248D | 1.22 | 0.43 | ||||||
| A1E | N76D | S103A | V104l | 1.12 | 0.32 | |||||||
| N76D | S103A | V104l | N261 D | 1.54 | 0.33 | |||||||
| N76D | S103A | V104l | S216C | 1.04 | 0.13 | |||||||
| N76D | N77D | S103A | V104l | A174V | 1.09 | 0.35 |
PL 191 529 B1 ciąg dalszy tabeli 4
| T38S | N76D | S103A | V104l | K237Q | 1.11 | 0.55 | ||||||
| N76D | S103A | V104l | N1830 | 1.50 | 0.25 | |||||||
| R19L | N76D | S103A | V104l | 1.11 | 0.48 | |||||||
| R19C | N76D | S103A | V104l | 1.05 | 0.19 | |||||||
| N76D | S103A | V104l | N184D | 1.32 | 0.29 | |||||||
| N76D | S103A | V104l | N252D | 1.19 | 0.53 | |||||||
| N76D | S103A | V104l | S259C | 0.92 | 0.12 | |||||||
| N76D | S103A | V104l | K251T | 1.31 | 0.43 | |||||||
| N76D | P86S | S103A | V104l | 1.00 | 0.98 | |||||||
| I72V | N76D | S103A | V104l | N1B5D | 1.70 | 0.37 | ||||||
| N76D | S103A | V104l | K237E | T274A | 1.12 | 0.16 | ||||||
| N76D | S103A | V104l | A228Y | 1.13 | 0.99 | |||||||
| N76D | S103A | V104l | G159D | 1.88 | 0.23 | |||||||
| H17L | N76D | S103A | V104l | K237E | 1.29 | 0.28 | ||||||
| N76D | S103A | V104l | S130L | 0.52 | 0.71 | |||||||
| N76D | S103A | V104l | Q109R | 0.23 | 1.26 | |||||||
| N76D | S99R | S103A | V104l | N204T | 0.21 | 0.87 | ||||||
| N76D | S103A | V104l | D181N | 0.24 | 1.07 | |||||||
| Q12R | N76D | S103A | V104l | 0.61 | 1.31 | |||||||
| N76D | S103A | V104l | S212P | E271Y | 0.69 | 1.35 | ||||||
| N76D | S103A | V104l | N252K | N261Y | 0.37 | 1.02 | ||||||
| N76D | S103A | Y104I | S242T | 0.98 | 0.92 | |||||||
| N76D | S103A | V104l | E271Q | 0.63 | 1.25 | |||||||
| Q12R | N76D | S103A | V104l | S242T | 049 | 1.32 | ||||||
| N43S | N76D | S103A | V104l | N116K | N1831 | 039 | 1.10 | |||||
| N76D | S103A | V104l | G258R | 034 | 1.17 | |||||||
| N76D | S103A | V104l | E271G | 0.57 | 1.25 | |||||||
| N76D | S103A | V104l | Q182R | 1198 V | 022 | 0.95 | ||||||
| L21M | N76D | S103A | V1Q4I | Q182R | 0.24 | 0.98 | ||||||
| N76D | S103A | V104l | M119I | Q137R | 0.13 | 0.91 | ||||||
| N76D | S103A | V104l | Q137R | N248S | 0.16 | 1.02 | ||||||
| A13T | N76D | S103A | V104l | Q206R | 0.31 | 1.01 | ||||||
| N76D | S103A | Y104I | Q206R | 0.33 | 1.02 | |||||||
| N76D | S103A | V104l | S212P | G258R | 0.38 | 1.06 | ||||||
| T58S | N76D | S103A | V104l | E271G | 0.84 | 1.26 | ||||||
| N76D | S103A | V104l | Q206E | N261D | 1.97 | 0.04 | ||||||
| V4E | N76D | S103A | V104l | Q206E | 1.51 | 0.05 |
PL 191 529 B1 ciąg dalszy tabeli 4
| N76D | N77D | S103A | V104l | Q206E | 1.40 | 0.04 | ||||||
| N76D | S103A | V104l | A158E | 1.95 | 0.16 | |||||||
| N76D | S103A | V104l | Q206E | 2.41 | 0.88 | |||||||
| N76D | N770 | S103A | V104l | A133T | N185D | K251T | 1.34 | 0.03 | ||||
| N76D | S103A | V104l | Q206E | N261 D | 1.78 | 0.04 | ||||||
| N76D | S103A | V104l | G159D | Q206E | V244A | 2.16 | 0.04 | |||||
| V4E | N76D | S103A | V104l | S188E | 1.91 | 0.04 | ||||||
| V4E | N76D | S103A | V104l | A158E | 2.06 | 0.04 | ||||||
| N76D | S103A | V104l | Q206E | K251T | 1.73 | 0.06 | ||||||
| A48T | N76D | S103A | V104l | L111M | G159D | 2.04 | 0.16 | |||||
| V68A | N760 | S103A | V104l | G159D | Q236H | 3.20 | 0.09 | |||||
| L42V | N76D | S103A | V104l | G159D | 1.83 | 0.17 | ||||||
| Q12H | N62 H | N76D | S103A | V104l | G159D | 1.42 | 0.14 | |||||
| L42I | N76D | S103A | V104l | G159D | 1.86 | 0.18 | ||||||
| N76D | S103A | V104l | G146S | G159D | 1.87 | 0.19 | ||||||
| N76D | S103A | V104l | G159D | N238S | 1.90 | 0.15 | ||||||
| N76D | S103A | V104l | G159D | T224A | 1.61 | 0.07 | ||||||
| N76D | S103A | V104l | S212P | Y268F | E271V | 0.44 | 1.42 | |||||
| N76D | S87R | S103A | V104l | S212P | E271Y | 0.39 | 2.03 | |||||
| N76D | S103A | V104l | S212P | Q245L | E271V | 0.62 | 1.79 | |||||
| N76D | S103A | V104l | Q109R | Q245R | 0.11 | 1.78 | ||||||
| N76D | S103A | V104l | Q109R | P210L | 0.12 | 1.21 | ||||||
| G20V | N62S | N76D | S103A | V104l | 1.63 | 0.78 | ||||||
| V68A | N76D | S103A | V104l | Q236H | 2.37 | 0.44 | ||||||
| V68A | N76D | S103A | V104l | G159D | Q236H | E271V | 2.97 | 0.45 | ||||
| V88A | N76D | S103A | V104l | G159D | Q236H | Q245R | 3.00 | 0.61 | ||||
| V68A | N76D | S103A | V104l | G159D | L217I | Q236H | E271V | 2.71 | 0.12 | |||
| H17Q | V68A | N76D | S103A | V104l | 2.46 | 0.38 | ||||||
| V68A | N76O | S103A | V104l | 2.46 | 0.61 | |||||||
| V68A | N76D | S103A | V104l | G159D | Q236R | 3.31 | 0.11 | |||||
| V68A | L75R | N76D | S103A | V104l | G159D | Q236H | 3.06 | 0.14 | ||||
| V68A | N76D | S103A | V104l | A114Y | V121l | G159D | Q236H | Q245R | 3.11 | 0.40 | ||
| Q12R | V68A | N76D | S103A | V104l | G159D | Q236H | 3.12 | 0.34 | ||||
| V68A | N76D | S103A | V104l | G159D | Y209S | Q236H | T253K | 3.18 | 0.03 | |||
| V68A | N76D | S103A | V104l | N117K | G159D | N184S | Q236H | 2.78 | 0.06 | |||
| V68A | N76D | S103A | V104l | Q236H | 2.49 | 0.57 | ||||||
| V68A | N76D | S103A | V104l | G159D | Q236H | Q245L | 3.37 | 0.03 |
PL 191 529 B1 ciąg dalszy tabeli 4
| V68A | N76D | S103A | Y104I | N123S | G159D | Q236H | H249Y | 3.11 | 0.03 | |||
| V68A | N76D | S103A | Y104I | G159D | Q236H | H249Q | 3.15 | 0.04 | ||||
| V68A | N76D | S103A | Y104I | G159D | Q236H | Q245R | N261 D | 3.31 | 0.03 | |||
| V68A | N76D | S103A | Y104I | S141N | G159D | Q236H | Q245R | T255S | 3.26 | 0.62 | ||
| V68A | N76D | S103A | Y104I | G159D | Q236H | Q245R | R247H | 2.78 | 0.03 | |||
| V68A | N76D | S103A | Y104I | G159D | A 174 V | N204D | Q236H | Q245R | 3.28 | 0.02 | ||
| V68A | N760 | S103A | Y104I | G159D | N204D | Q236H | Q245R | 3.34 | 0.02 | |||
| V68A | N76D | S103A | V104l | A133V | G159D | N218D | Q236H | Q245R | 3.28 | 0.03 | ||
| V68A | N76D | S103A | Y104I | G159D | A232V | Q236H | Q245R | 2.91 | 0.58 | |||
| V68A | N76D | S103A | Y104I | G159D | A194I | Y203A | Q236H | Q245R | 2.86 | 0.13 | ||
| V68A | N76D | S103A | Y104I | G159D | T213R | A232V | Q236H | Q245R | T260A | 1.30 | 1.73 | |
| T22K | V68A | N76D | S103A | V104l | 1.83 | 1.13 | ||||||
| V68A | N76D | S103A | V104l | G159D | P210R | A232V | Q236H | Q245R | 1.28 | 1.54 | ||
| V68A | N76D | S103A | Y104I | G159D | A232V | Q236H | Q245R | L257V | 3.72 | 0.8 | ||
| N76D | S103A | V104l | A232Y | Q236H | Q245R | L257V | 0.6 | 1.5 | ||||
| N76D | S103A | V104l | L257Y | R275H | 1.91 | 0.15 | ||||||
| N76D | S103A | V104l | G159D | A232Y | Q236H | Q245R | L257V | 1.92 | 1.09 | |||
| V68A | N76D | S103A | V104l | G159D | Y209W | A232V | Q236H | Q245R | 3.57 | 0.99 | ||
| V68A | N76D | S103A | V104l | G159D | G211R | A232V | Q236H | Q245R | 1.74 | 1.76 | ||
| VB8A | N76D | S103A | V104l | G159D | G211Y | A232V | Q236H | Q245R | 3.15 | 1.06 | ||
| Q12R | V68A | N76D | S103A | V104l | G159D | Y214L | A232V | Q236H | Q245R | 2.33 | 1.92 | |
| V68A | N76D | S103A | V104l | G159D | A215R | A232V | Q236H | Q245R | 1.67 | 1.45 | ||
| Q12R | V68A | N76D | S103A | V104l | G159D | A232V | Q236H | Q245R | 2.16 | 1.72 | ||
| G20R | V68A | N76D | S103A | V104l | G159D | A232V | Q236H | Q245R | S259G | 2.77 | 1.59 | |
| V68A | N76D | S87R | S103A | V104l | G159D | A232V | Q236H | Q245R | T260Y | 2.62 | 1.49 | |
| V68A | N76D | S103A | V104l | G159D | A232V | Q236H | Q245R | N261G | 2.92 | 0.68 | ||
| V68A | N78D | S103A | V104l | G159D | A232V | Q236H | Q245R | N261 W | 2.17 | 1.37 | ||
| N76D | S103A | V104l | A232V | Q236H | S242P | Q245R | 0.48 | 1.2 | ||||
| V68A | N76D | S103A | V104l | G159D | P210L | A232V | Q236H | Q245R | 2.92 | 0.76 | ||
| Q12R | A48V | V68A | N76D | S103A | V104l | G159D | A232V | Q236H | Q245R | 2.09 | 1.86 | |
| N76D | S103A | V104l | A232V | Q236H | Q245R | 0.51 | 1.44 | |||||
| N76D | S103A | Y104I | G159D | Y192F | A232V | Q236H | Q245R | 1,60 | 1.14 | |||
| N76D | S103A | V104l | Y147I | G159D | A232V | Q236H | Q245R | N248S | K251R | 1.35 | 1.29 | |
| Q12R | V68A | N76D | S103A | V104l | G159D | A232V | Q236H | Q245R | A272S | 1.92 | 1.81 | |
| V68A | N76D | S103A | V104l | G159D | N183K | Q206L | A232V | Q236H | Q245R | 1.17 | 1.53 | |
| V68A | N76D | S103A | V104l | G159D | A232V | Q236H | Q245R | S256R | 2.01 | 1.72 | ||
| V68A | N76D | S103A | V104l | G159D | Q206R | A232V | Q236H | Q245R | 2.09 | 1.62 |
PL 191 529 B1 ciąg dalszy tabeli 4
| K27R | V68A | N76D | S103A | V104l | G159D | A232V | Q236H | Q245R | 3.00 | 1.08 | ||
| V68A | N76D | S103A | V104l | N116T | G159D | R170S | N185S | A232V | Q236H | Q245R | ND | ND |
| N76D | S103A | V104l | M222S | Q245R | 1.01 | 1.23 | ||||||
| Q12R | N76D | S103A | V104l | M222S | H249R | 0.57 | 1.65 | |||||
| N76D | S103A | V104l | N173R | M222S | 0.86 | 0.46 | ||||||
| N76D | S103A | V104l | M222S | Y263F | 1.24 | 0.77 | ||||||
| L21M | N76D | S103A | V104l | M222S | K237R | Y263F | 1.18 | 0.76 | ||||
| N76D | S103A | V104l | Q109R | M222S | 0.52 | 1.16 | ||||||
| N76D | S103A | V104l | Q109R | M222S | E271D | 0.56 | 1.12 | |||||
| G61R | N76D | S103A | V104l | M222S | 0.43 | 0.96 | ||||||
| N76D | S103A | V104l | Q137R | M222S | 0.42 | 1.25 | ||||||
| N76D | S103A | V104l | M222S | H249R | 1.15 | 1.01 | ||||||
| Q12R | N76D | S103A | V104l | M222S | Q245R | 0.53 | 1.46 | |||||
| N76D | S103A | V104l | A232Y | Q245R | 0.69 | 1.56 | ||||||
| Q12R | N76D | S103A | I104T | M222S | V244l | Q245R | 0.66 | 1.74 | ||||
| Q12R | N76D | S103A | V104l | M222S | P210T | Q245R | 0.52 | 1.56 | ||||
| Q12R | N76D | S103A | I104T | S130T | M222S | Q245R | 0.70 | 1.61 | ||||
| Q12R | N76D | S103A | I104T | S130T | A215V | M222S | Q245R | 0.79 | 1.85 | |||
| Q12R | N76D | S103A | I104T | S130T | M222S | Y227A | Q245R | L262S | 0.78 | 1.56 | ||
| Q12R | N76D | S103A | I104T | S130T | M222S | Q245R | N261 D | 1.25 | 1.30 | |||
| N76D | S103A | I104T | S130T | M222S | Q245R | 1.29 | 1.30 | |||||
| Q12R | S57P | N76D | S103A | I104T | S130T | M222S | Q245R | K251Q | 1.44 | 0.16 | ||
| Q12R | N76D | S103A | I104T | S130T | R170S | N185D | M222S | N243D | Q245R | 2.01 | 0.04 | |
| Q12R | N76D | S103A | I104T | S130T | M222S | Q245R | Y268A | 0.77 | 1.60 | |||
| Q12R | N76D | S103A | I104T | S130T | M222S | P210S | Q245R | 0.73 | 1.66 | |||
| V68A | N76D | S103A | Y104I | G159D | A232Y | Q236H | Q245R | 2.09 | 0.86 |
Pr zy kła d 3
W tabeli 5 podano wyniki badania proteaz z przykładu 1 w czterech różnych środkach piorących. Badania wykonano, jak w przykładzie 2. Do badań, których wyniki przedstawiono w poszczególnych kolumnach użyto następujących środków piorących: kolumna A - 0,67 g/l filtrowanego środka piorącego Ariel Ultra (Procter & Gamble, Cincinnati, OH, USA) w roztworze o mieszanej twardości wapniowo/magnezowej w wysokości 3 gramów na 4 litry oraz 0,3 ppm enzymu w każdej studzience w temperaturze 20°C; kolumna B - 3,38 g/l filtrowanego środka piorącego Ariel Futur (Procter & Gamble, Cincinnati, OH, USA) w roztworze o mieszanej twardości wapniowo/magnezowej w wysokości 15 gramów na 4 litry oraz 0,3 ppm enzymu w każdej studzience w temperaturze 40°C; kolumna C - 3,5 g/l środka piorącego HSP1 (Procter & Gamble, Cincinnati, OH, USA) w roztworze o mieszanej twardości wapniowo/magnezowej w wysokości 8 gramów na 4 litry oraz 0,3 ppm enzymu w każdej studzience w temperaturze 20°C; kolumna D - 1,5 ml/l środka piorącego Tide KT (Procter & Gamble, Cincinnati, OH, USA) w roztworze o mieszanej twardości wapniowo/magnezowej w wysokości 3 gramów na 4 litry oraz 0,3 ppm enzymu w każdej studzience w temperaturze 20°C.
PL 191 529 B1
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| Ω | a | a | o | a | o | X | Ω | Ω | n | n | ΓΊ | n | Ω | Q | ||||
| 00 | 00 | 00 | 00 | 00 | 00 | io | 00 | 00 | 00 | 00 | 00 | CN | 00 | 00 | 00 | |||
| Ν’ | Tf | Ν’ | Tf- | LO | ||||||||||||||
| CN | CN | CN | CN | CN | CN | CN | CN | CN | CN | CN | CN | CN | CN | CN | CN | |||
| Z | Z | z | z | Z | z | o | z | Z | z | z | Z | Z | z | Z | Z | |||
| X. | X | IX | X | x | x | a: | X | X | X | X | X | X | Q | X | X | ct | ||
| CN | m | io | LO | LO | O | LO | CD | LO | LO | LO | LO | LO | 00 | IO | LO | iO | ||
| ιο | Ν’ | 5T | sf | CO | 5T | 5Γ | Ν’ | N- | ||||||||||
| CN | CN | CN | CN | CN | CN | CN | CN | CN | CN | CN | CN | CN | CN | CN | CN | CN | ||
| Z | o | o | a | o | σ | σ | o | o | o | σ | O | σ | Z | o | a | σ | ||
| Ω | I | X | X | X | X | X | > | X | X | X | X | X | X | X | X | X | ||
| 00 | CD | CO | CD | CD | CD | CN | CD | CD | co | CD | co | iO | CD | co | CD | |||
| 5f | CO | co | CO | CO | CO | CO CN | CO | CO | co | CO | co | Ν’ | CO | co | CO | |||
| CN | CN | CN | CN | CN | CN | CN | CN | CN | CN | CN | CN | CN | CN | CN | ||||
| Z | o | o | o | o | σ | o | < | σ | σ | o | o | σ | σ | σ | σ | σ | ||
| a: | > | >- | > | > | >- | >- | _J | >- | > | > | > | >- | X | > | > | > | ||
| CN | CN | CN | CN | CN | CN | o | CN | CN | CN | CN | CN | CD | CN | CN | CN | |||
| CO | CO | CO | CO | CO | CO | CO | CO | CO | CO | CO | CO | CO | CO | CO CN | ||||
| CN | CN | CN | CN | CN | CN | CN | CN | CN | CN | CN | CN | CN | CN | CN | ||||
| a | < | < | < | < | < | < | 0- | < | < | < | < | < | σ | < | < | < | ||
| x | § | o | Ω | U- | a | X | ω | —1 | o | o | 111 | UJ | > | UJ | >- | |||
| 05 | 05 | 05 | 05 | lo | o | io | o | CN | CN | CN | co | CN | co | LO | ||||
| O | O | 00 | 00 | CO | ||||||||||||||
| CN | CN | CN | CN | CN | CN | CN | CN | CN | CN | |||||||||
| o | > | O | o | > | Z | CL | z | 0. | W | 00 | W | P | < | 1- | < | |||
| > | a | Q£ | Ω | a | Ω | Ω | Ω | Ω | a | o | Ω | co | n | n | n | |||
| CN | 05 | Ν’ | 05 | 05 | 05 | 05 | 05 | 05 | 05 | 05 | 05 | 05 | 05 | 05 | ||||
| CO | lO | O | O | io | LO | lO | iO | LO | LO | LO | IO | LO | LO | lO | LO | |||
| CN | ||||||||||||||||||
| < | o | O | > | o | o | o | o | O | o | O | o | o | 1- | o | o | o | ||
| Q 05 | Ν' | $ | Ν’ | N | Ν’ | Ν’ | Ν' | |||||||||||
| ο | o | O | o | o | O | o | O | o | O | o | O | o | o | o | o | o | ||
| > | o | > | > | co | > | > | > | > | > | > | > | > | > | > | > | > | > | |
| < | < | < | < | < | < | < | < | < | < | <r | <r | < | >- | < | < | |||
| CO | co | co | < | co | CO | co | co | co | co | co | co | CO | co | co | co | co | ||
| ο | O | o | o | co £ | o | O | o | o | o | o | o | o | o | o | o | o | o | |
| 00 | > | ω | 00 | w | w | co | ω | w | w | w | w | w | w | < | w | ώ | ||
| Ω | 3A | < | < | X | < | < | < | < | < | < | < | < | < | < | < | < | < | |
| CD | O | 00 Ϊ | 00 | 00 | oo | DO | 00 | 00 | 00 | oo | 00 | DO | 00 | oo | 00 | co | ||
| CD i | CD | CD | to | to | to | CD | CD | CD | to | to | to | to | co | CD | ||||
| Z | 00 | > | > | o | > | > | > | > | > | > | > | > | > | > | > | > | > |
PL 191 529 B1 ciąg dalszy tabeli 5
| α | co | r~- | OT | CN | LO | ΓΝ | LO | CN | CN | LO | co | LO | oo | Ν' | CD | N. | ||||
| CN | ot | oo | «5 | Ν' | OT | Ν' | co | CN | r- | CD | OT | OT | O | t£5 | co | r- | CD | IO | Ν’ | |
| τ- | T- | *- | CN | CN | O | CN | CD | ί- | «- | csi | O | i- | CN | O | O | CN | csi | |||
| co | K. | OT | Ν' | CN | o | CD | 00 | OT | CD | CO | lO | O | r~ | CN | CD | |||||
| Ν; | ot | N· | O | CN | CN | Ν' | <D | N | OT | LO | CD | N | cD | oo | CD | CD | CD | Ν’ | io | CD |
| *- | T- | «- | »“ | »— | V | O | Ί1-*- | ł- | x— | 5- | π— | τ- | ||||||||
| 00 | co | CD | CD | <D | co | O | cD | CO | CD | r- | CN | OT | Π-- | O | CD | co | LO | |||
| tO | CD | CD | cD | N | N | O | OT | CD | N | r- | LO | Ν’ | Ν’ | tO | Ν' | LO | OT | CD | CN | |
| *“ | O | O | *- | O | τ- | o | 1“ | 1- | T- | O | V- | 1- | O | Ί- | ci | |||||
| <Ν | CN | «O | Ν' | CD | <O | CN | LO | 00 | Ν' | CD | CD | CN | Ν' | O | CD | |||||
| <Ν | co | CN | N | OT | LO | O | (O | N | CD | CN | LO | LO | O | O | CD | CD | oo | |||
| CN | «- | CN | CN | O | CN | CN | CN | CN | csi | ΓΝ | »- | csi | τ- | *- | csi | csi | csi | |||
| Z | ||||||||||||||||||||
| CN | ||||||||||||||||||||
| LO | ||||||||||||||||||||
| CN | ||||||||||||||||||||
| Z | ||||||||||||||||||||
| Ζ | Z | Z | Z | Z | * | LL | > | z | III | rr | rr | tt | Q | X | V | n | ||||
| <Ν | CN | CN | CN | CN | CN | CN | LO | tp | cp | CD | co | CN | 40 | |||||||
| m | OT | CO | LO | io | LO | LO | OT | OT | OT | LO | iO | co | LO | |||||||
| CN | <N | CN | CN | CN | CN | CN | CM | CN | CN | CN | CN | CN | CN | CN | ||||||
| <L | ć. | Λ | ć. | Z | Z | H | OT | OT | W | H | l | 0 | Z | Z | Z | |||||
| α | a | a | O | D | □ | U- | _1 | Q | Z | z | Z | Z | z | Z | z | Z | o | er | ||
| oo | co | 00 | co | CO | co | CN | CN | 00 | CN | CN | CN | CN | CN | CN | CN | CN | CN | oo | LO | |
| Ν' | N | ’Τ | Ν' | Ν’ | N | CO | LO | N | LO | LO | LO | LO | LO | LO | Ό | LO | IO | Ν' | Ν' | |
| CN | CN | CN | CN | CN | CN | <N | CN | CN | CN | CN | CN | CN | CN | CN | CN | CN | CN | CN | CN | |
| Z | z | Z | Λ | Z | Z | Z | Z | Z | Z | Z | Z | Z | Z | Z | Z | Z | Z | Z | α | |
| Di | Di | oi | oi | Di | Di | n | n | (£ | n | n | n | n | n | n | o | Q | Z | n | rr | T |
| OT | CO | LO | LO | OT | OT | co | co | LO | 00 | co | co | co | 00 | oo | co | co | CN | co | LO | CD |
| Ν' | N | Ν' | Ν' | Ν' | Ν' | Ν' | Ν’ | N | Tt | N | N | N | N- | N- | Ν' | N1 | LO | Ν' | Ν' | CD |
| CN | CN | CN | CN | CN | CN | CN | CN | CN | CN | CN | CN | CN | CN | CN | CN | CN | CN | CN | CN | CN |
| O | <J | o | o | σ | o | Z | Z | o | Z | Z | Z | Z | Z | Z | Z | Z | Z | Z | o | o |
| I | X | T | X | X | X | rr | rr | T | rr | rr | cc | rr | rr | rr | rr | rr | Q | > | T | > |
| CD | CD | co | to | co | co | ot | OT | (O | OT | OT | LO | LO | LO | LO | LO | to | co | LO | CD | |
| CD | CD | CD | CD | CD | CD | Ν' | V | CD | Ν' | N | Ν' | N | Ν' | Ν' | Ν' | Ν' | N | Ν' | CD | CD |
| CN | CN | CN | CN | CN | CN | CN | CN | CN | CN | CN | CN | CN | CN | CN | CN | CN | CN | CN | CN | CN |
| υ | o | O | O | α | O | o | σ | o | o | o | σ | σ | σ | σ | σ | σ | Z | σ | σ | |
| > | > | > | > | > | > | X | X | > | X | X | X | X | X | X | X | X | X | X | > | n |
| CN | CN | CN | CN | CN | CN | co | (O | CN | CŁJ | CD | CD | CD | CD | to | Hi | co | LO | to | OT | |
| CD | cD | rD | CD | CD | CD | CD | CD | CD | CD | CD | CD | CD | CD | CD | Ν' | CD | LO | |||
| CN | CN | CN | CN | CN | ΓΝ | CN | CN | CN | CN | CN | CN | CN | CN | CN | CN | |||||
| < | < | < | < | •i | o | o | < | o | a | o | o | o | σ | o | 0 | a | α | 0 | ||
| oi | ł— | > | o | o | rr | > | > | O | > | > | > | > | > | > | > | > | X | > | > | < |
| OT | CD | co | CD | CD | co | CN | CN | OT | CN | CN | CN | CN | CN | CN | CN | CN | Φ | CN | oo | O |
| c* | o | CD | CD | OT | σ) | CD | CD | CD | CD | CD | CD | CD | CD | CD | CN | CD | ||||
| cn | CN | CN | CN | CN | (sj | CN | CN | CN | CN | CN | CN | CN | CN | CN | cy | ry | ||||
| < | ot | ot | ot | Z | O | < | < | 0 | < | < | < | < | < | < | < | < | U | < | < | OT |
| o | o | o | □ | □ | Q | Q | n | ΓΊ | n | n | n | n | n | n | n | > | n | n | ||
| ot | <n | 05 | O> | OT | OT | OT | OT | OT | OT | OT | OT | OT | OT | OT | OT | CN | OT | OT | ||
| OT | co | LO | m | LO | LO | LO | LO | o | LO | LO | LO | LO | LO | LO | LO | LO | CD | LO | LO | O |
| 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | > | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | > | |
| 041 | 3 | 3 | 3 | N O | Ν' O | Ν' O | Ν' O | νεο | Ν' O | 3 | 3 | 3 | 3 | Ν' O | Ν' σ | Ν' O | Ν' O | N O | Ν’ O | 03A |
| > | > | > | > | > | > | > | > | OT | > | > | > | > | > | > | > | > | > | > | > | W |
| s | 3A | s | s | $ | $ | s | 3A | < | < co | < CD | S | S | 3A | s | $ | s | i | < | ||
| o | O | o | o | O | o | o | O | 00 | o | O | O | O | O | o | o | o | o | o | o | 00 |
| tp | ||||||||||||||||||||
| ot | w | w | w | w | ot | w | OT | > | V) | OT | OT | OT | OT | OT | OT | OT | W | OT | OT | > |
| < | < | < | < | < | < | < | < | cn | < | < | < | < | < | < | <C | <T | LU | |||
| <o | oo | co | co | co | w | 00 | 00 | LO | 00 | 00 | OO | co | CO | oo | 40 | CO | w | |||
| CD | CD | SP | co | tp | (D | SP | CD | u> | ID | (O | CD | CD | CD | co | «0 | CD | CD | CD | ||
| > | > | > | > | > | > | > | > | Q_ | > | > | > | > | > | > | > | > | > | > | > | o |
PL 191 529 B1 ciąg dalszy tabeli 5
| σ> | r- | 8 | id | «3 | ID | 00 | id | ID | O | CM | o | co | Ol | o | co | cO | 3 | |||
| CM | CM | CM | <M | CM | M- | uD | r- | O) | co | CD | i*- | M- | ID | co | ||||||
| CM | *“ | T- | CM | T- | r- | «- | T- | CM | CM | CM | 1- | CM | CM | |||||||
| ΙΟ | <T> | CM | r- | CO | ||||||||||||||||
| CO | co | CO | co | x— | M· | Cł | O | Q | O | a | O | o | n | O | a | O | O | n | O | |
| — | o | *“ | *- | *“ | »- | z | Z | Z | Z | z | Z | z | z | Z | z | Z | z | Z | z | Z |
| h- | o | O | CM | O | co | r- | o | O) | 00 | oo | r- | o | co | D | iD | <31 | co | CD | ||
| σι | D | m | l·- | co | co | o | co | h- | ω | to | CM | co | <o | m | O | to | ||||
| o | »“ | »- | ▼- | *- | o | o | o | O | O | ó | o | O | i- | -f- | o | 1- | CM | CM | ci | 1- |
| o | h- | o | CM | CM | o | o | h- | CM | 1— | o | uf) | a> | CM | iD | ID | CM | co | |||
| T- | <O | co | [— | co | tf> | CM | CO | r- | O) | tfł | CM | CO | co | CM | h- | M- | M- | |||
| CM | <r- | CM | 1- | CM | M- | co | -T | co | CM | c\i | CO | CM | co | M- | M1 | id | CO | l< | iD | |
| < | ||||||||||||||||||||
| O | ||||||||||||||||||||
| CD | ||||||||||||||||||||
| CM | ||||||||||||||||||||
| H | ||||||||||||||||||||
| X | Y | γ | id | |||||||||||||||||
| U) | CM | CM | CM | |||||||||||||||||
| 'ł | in | ID | ŁD | |||||||||||||||||
| CM | CM | CM | CM | |||||||||||||||||
| O | Z | Z | Z | |||||||||||||||||
| id | γ | id | I | Y | n | n | n | |||||||||||||
| CM | CM | CM | <D | CM | GO | GO | 00 | |||||||||||||
| in | LD | iD | co | UD | M* | Μ- | M | |||||||||||||
| CM | CM | CM | CM | CM | CM | CM | CM | |||||||||||||
| Z | Z | Z | O | Z | Z | Z | Z | |||||||||||||
| Q | Si | Q | O | > | O | X | X | X | Y | Y | Y | Z | Y | Y | X | Y | Y | id | ||
| OT | CM | GO | 03 | CM | co | io | Li) | ID | CM | CM | CM | CM | CM | CM | CM | CM | CM | CM | CM | |
| •M· | id | fl· | CO | M· | M- | M- | M- | ID | ID | ID | ID | ID | ID | iD | iD | <D | ID | ID | D | |
| CM | CM | CM | CM | CM | <M | CM | CM | CM | CM | CM | CM | CM | CM | <M | CM | CM | CM | CM | CM | CXI |
| Z | Z. | Z | Z | < | Z | σ | o | a | Z | Z | Z | Z | Z | Z | Z | Z | Z | Z | Z | Z |
| IX | o | (X | X | X | X | z | Z | z | a | n | n | Q | a | a | a | o | Y | a | n | n |
| 10 | ao | LO | kO | co | tf> | 8 | 8 | ID | oo | 03 | oo | 00 | ao | oo | co | co | CM | co | co | oo |
| M· | M- | M· | M- | M- | to | M- | Μ | ID | M | |||||||||||
| CM | CM | CM | CM | CM | CM | CM | CM | CM | CM | CM | CM | CM | CM | CM | CM | CM | CM | CM | CM | CM |
| O | Z | U | σ | H | σ | y | y | a | Z | Z | Z | Z | Z | Z | Z | Z | Z | Z | Z | Z |
| I | X | T | Z | z | > | > | > | X | X | X | X | X | X | X | X | n | X | X | X | |
| co | iD | co | co | o | 8 | CM | CM | ID | UD | ID | ID | ID | ID | ID | D | CO | ID | ID | ID | |
| co | M- | co | <*> | CO CM | co cm | CO | Μ- | TT | *3 | rf | TT | M- | M- | 5t | ||||||
| CM | CM | CM | CM | CM | CM | CM | CM | CM | CM | CM | CM | CM | <M | CM | CM | <M | CM | CM | ||
| O | U | u | y | Ł | O | i | < | < | y | u | u | υ | o | y | σ | y | Z | y | u | y |
| > | Z | > | ·> | O | > | > | LL | Z | z | z | Z | z | Z | Z | X | Z | T | Z | ||
| 232 | co co | 232 | CM CO | (31 ID | CM CO | co | M o | r·- (D | 8 | CO CO | 8 | 8 | 8 | CD CO | <D CO | (D CO | ID Μ- | <D CO | CD CO | to ΓΟ |
| CM | CM | CM | CM | CM | CM | CM | CM | CM | CM | CM | CM | CM | CM | CM | ||||||
| < | o | < | < | 0 | < | co | > | > | u | CO | u | y | o | o | O | σ | o | σ | O | σ |
| a | > | CO | a | a | Q | < | Q | > | > | > | > | > | > | > | > | z | > | > | > | |
| σ> | CM | 00 | O) | 3 | σι | σι | co | 2 | CM | CM | CM | CM | CM | CM | CM | CM | CO | CM | ΓΜ | CM |
| UD | CO | kfi | ID | m | O | ID | CO | (O | CO | CO | CO | CO | CO | CO | co | <O | (O | CO | ||
| CM | CM | CM | CM | CM | CM | CM | <M | <M | CM | 3 | <M | |||||||||
| 0 | < | Z | 0 | > | 0 | 0 | <0 | 0 | < | < | < | < | < | < | < | < | u | 3 | < | |
| Q | o | <r | a | Q | a | Q | Cl | a | o | o | > | Q | O | a | ||||||
| co | cn | en | 3 | 3 | a | <n | O) | 05 | cn | cn | <31 | <31 | <7) | <3ł | rt> | |||||
| co | m | Ul | a | o | (D | O | iD | ID | ID | iD | lO | <D | iD | iD | co | ID | ID | co | ||
| < | □ | δ | > | ώ | > | > | Z | > | o | o | a | o | C5 | u | u | u | 3 | o | o | Z |
| 1041 | 1041 | 1041 | I03A | E89D | I03A | I03A | ś § | I03A | 1041 | 1041 .. 1 | 1041 | 1041 | 1041 | 1041 | I06E | LU σι o | Q <31 ID | X g | 041 | | 1590 |
| > | > | > | CO | ca | <0 | co | > | > | > | > | > | > | ω | σ | o | σ | > | O | ||
| < | < | < | UJ | < | < | < | < | < | < | |||||||||||
| co | ro | co | □ | O | <C | co | cO | co | co | co | co | 3 | 3 | ’Τ | co | |||||
| o | o | o | CD | CD | CO | O | O | O | O | o | O | o | O | o | O | |||||
| T— | Γ*- | r- | co | |||||||||||||||||
| co | co | co | > | Z | z | > | 0 | > | w | CD | 0 | <D | 0 | ω | > | > | > | > | ω | > |
| LLJ | < | < | < | < | 111 | LU | LU | □ | LU | UJ | o | 2A | s | s | s | a | < co | |||
| 00 | BO | co | oo | —l | r*- | s | s | O | o | O | o | o | CM | o | ||||||
| CD | CD | CO | CM | CD | co | CD | co | CD | O) | CD | ||||||||||
| O | > | > | 0 | > | > | 0 | co | 0 | 0 | < | ω | <0 | CD | 0 | w | w | w | ω | Z | W |
PL 191 529 B1 ciąg dalszy tabeli 5
| κ | D3 | o | s | o | ot | o | CD | OT | l·- | CN | Tf | CD | cn | CN | OT | co | 3 | cn | r*. | |
| ο | ο | (O | T- | OT | Tf | OO | ot | Tf | CD | Tf | cn | CO | cn | to | O | co | ||||
| to | tO | CN | CN | T- | CN | CN | T- | T- | CN | T- | T- | T- | OT | T- | T- | T- | CN | CO | có | CN |
| Ο | Ω | Ω | Ω | Ω | Ω | Ω | Q | n | O | a | Π | O | n | n | n | n | n | n | n | Ω |
| Ζ | Ζ | Z | Z | z | Z | Z | Z | z | Z | z | Z | Z | z | z | z | z | z | z | z | Z |
| ot | co | CN | co | 00 | Tf | CO | OT | CO | co | Tf | r~- | to | σ | o | o | h- | ||||
| CN | cn | OD | OT | co | Tf | o | O | t~ | to | o | co | r- | di | Tf | OT | ou | OT | CN | co | |
| τ- | ο | CN | *“ | CN | CN | CN | CN | CN | T- | CN | CN | CN | o | «- | Ó | o | -- | csi | CN | |
| CM | co | r- | CO | to | OT | CN | 3 | Tf | O | CO | t— | Tf | Tf | f- | CN | CD | oo | |||
| τ~ | co | t— | co | O | O | co | CN | oo | co | CO | O | r- | Oł | •4 | ’Τ | CO | co | OT | ||
| ιη | co | co | Tf | co | Tf | CN | CN | CN | CN | CN | CN | CN | f- | OT | CN | Tf | OT | OT | CN | |
| σ | ||||||||||||||||||||
| A- | ||||||||||||||||||||
| CN | ||||||||||||||||||||
| LU | ||||||||||||||||||||
| V | ||||||||||||||||||||
| CN | ||||||||||||||||||||
| OT | ||||||||||||||||||||
| CN | ||||||||||||||||||||
| Z | ||||||||||||||||||||
| ¥ | (X | Ω | ||||||||||||||||||
| CN | CO | IN | CU | CN | ||||||||||||||||
| IO | OT | OT | 4· | OT | ||||||||||||||||
| CN | CN | CN | tN | CN | ||||||||||||||||
| Z | OT | Z | Z | Z | ||||||||||||||||
| Ω | Z | ki | * | o | IX | V | * | Ω | ||||||||||||
| co | CN | CN | CN | CN | ao | OT | CN | CN | CN | CN | CN | CN | co | |||||||
| Tf | ot | OT | OT | OT | 4- | Tf | OT | OT | OT | OT | OT | OT | ||||||||
| CN | CN | CN | CN | CN | CN | CN | CN | CN | CN | CN | CN | CN | CN | |||||||
| Z | Z | Z | Z | Z | Z | O | Z | Z | Z | Z | Z | Z | Z | |||||||
| 5Χ | 5Χ | cx | Ω | Ω | Ω | X | V | ¥ | V | * | n | IX | X | n | n | o | n | n | n | rr |
| CN | CN | LO | 00 | oO | oO | CN | CN | CN | CN | CN | co | OT | <o | oo | 03 | oo | oo | oo | co | OT |
| m | ot | Tf | Ν’ | Tf | Tf | ot | ot | OT | to | LO | Tf | Tf | co | Tf | Tf | Tf | Tf | Tf | ||
| CN | CN | CN | CN | CN | ΓΝ | CN | CN | CN | CN | CN | CN | CN | <N | CN | CN | CN | CN | CN | CN | CN |
| Ζ | Z | σ | Z | Z | Z | Z | Z | Z | Z | Z | Z | O | O | Z | Z | Z | Z | Z | Z | ϋ |
| Ω | O | X | £X | [X | (X | Ω | Ω | a | a | n | rr | X | > | 0£ | ir | LX | (X | rr | rr | X |
| m | co | co | OT | to | ot | OO | OO | co | 00 | co | OT | £ | OT | OT | OT | OT | OT | OT | to | |
| ν· | f | co | •=f | 3· | Tf | Tf | Tf | Ν' | Tf | Tf | co CN | Tf | Tf | Tf | Ν’ | tO | ||||
| ί\| | CN | CN | CN | CN | CN | CN | CN | CN | CN | CN | CN | CN | CN | CN | CN | CN | CN | CN | CN | |
| Ζ | Z | o | σ | u | U | Z | Z | Z | Z | Z | u | a | a | O | o | O | o | σ | σ | |
| α: | IX | > | X | X | X | cx | ix | X | ix | IX | X | (Y | X | X | X | X | X | X | > | |
| OT | ot | CN | co | CD | £ | OT | to | IO | U) | U) | co | CN | to | to | to | to | to | to | ||
| S | xr | CO | co | co | Tf | Tf | Tf | Tf | Tf | CO | CO | to | to | σ> | σ) | to | to | |||
| (Ν | CN | CN | CN | CN | CN | CN | CN | CN | CN | CN | CN | CN | tN | tN | CN | CN | tN | |||
| α | O | < | u | U | O | U | U | O | a | a | O | < | 1- | O | O | O | O | σ | a | < |
| τ | X | rr | > | > | > | X | H | < | X | I | > | tx | LU | > | > | > | > | > | > | o |
| to | £ | CO | CN | CN | CN | co | Tf | (O | to | CN | co | cn | ΓΝ | CN | CN | |||||
| co | co | CO | Tf | N- | co | to | co | σ | co CN | to CN | (O | CO | co | to CN | ||||||
| CN | CN | A2 | cn | <N | CN | CN | CN | CN | CN | cn | tN | CN | CN | CN | ||||||
| σ | O | H | < | < | O | > | > | U | U | < | H | U | < | < | < | < | < | < | OT | |
| > | > | Ω | rx | LU | (X | > | X | X | > | cx | Ω | n | n | LU | σ | o | n | o | n | |
| CN | CN | OT | (O | co | CN | £ | £ | CN | CN | co | OT | OT | OT | <n | (T) | OT | CN | OT | ||
| ίΟ | cO | ot | o | co | co | CO | OT | 00 | 00 | o | OT | OT | OT | |||||||
| cn | C\| | CN | 3 | CN | CN | CN | CN | CN | cn | CN | ||||||||||
| < | < | O | H | U | < | U | O | < | < | P | □ | Z | Z | o | P | o | o | OT | o | |
| Ω | LU | Ω | X | Ω | o | >- | > | Ω | Ω | Ω | Ω | Ω | Ω | n | O | |||||
| to CO | r- | Tf O | OT IO | to o | σι to | 3 | CN CO | CN co | 03 to | OT «Ο | cn OT | 3 | u> OT | 03 OT | OT OT | OT OT | 3 | 3 | OT OT | 3 |
| CN | CN | CN | CN | |||||||||||||||||
| ω | l | > | O | U | O | Z | < | < | o | O | o | > | o | O | O | o | > | > | O | > |
| Ο σ> ot | 159D | I03A | I04I | 159D | I04I | 159D | Ω σ> OT | 159D | I04I | I04.I | 1041 | I03A | ,04, J | 1041 | 1041 | 1041 | I03A | νεο. | 1041 | I03A |
| Ο | O | OT | > | O | > | O | O | O | > | > | > | OT | > | > | > | > | OT | OT | > | OT |
| < | <r | <r | <r | <T | < | < | £ | < | ||||||||||||
| Tf | Ω | co | I04 | co | Tf | co | co | CO | Ω | co | <o | CN | CN | CN | ||||||
| ο | o | CN | o | O | o | o | a | o | o | o | N62 | o | o | o | o | O | O | O | O | |
| > | > | Z | ω | > | w | > | > | > | M | OT | w | OT | OT | OT | OT | o | O | OT | O | |
| $ | s | IX | Ω | s | Ω | a | 3A | s | Z | O | Ω | X | n | O | o | o | _J | O | 2A | rr |
| ο | o | o | CN co z | o | CN CD Z | o | O | o | r* | co | N62 | CN | CN CO Z | o | o | o | co | o | O | CN |
| ω | ω | o | ot | OT | CO | (Λ | 2 | 1- | σ | OT | OT | OT | < | OT | o | σ |
PL 191 529 B1 ciąg dalszy tabeli 5
| 2.25 | CO o CN | m CN CN | 2.34 | CO CO | I1-49 | ao U3 CN | 5 | 0 03 | to p | 1X3 03 CN | r- r- | 1X3 N | 3.05 | co 0 | O CN | 0 | co | 03 p | 1X3 p | 03 CN | 0 | 1.25 |
| α | O | α | 0 | O | O | Cl | Cl | Cl | Cl | Cl | O | 0 | Cl | Cl | a | O | Q | η | a | O | n | a |
| z | Z | z | Z | Z | Z | Z | z | z | Z | Z | Z | Z | Z | Z | z | Z | Z | z | z | Z | z | z |
| r~ | CD | <O | O | 10 | CN | 03 | 03 | co | h- | co | n- | 03 | co | 03 | CD | 0 | 0 | |||||
| ν- | o | Ν' | r- | T | 03 | K | 03 | CN | r*- | CO | 03 | O | CO | N- | 03 | CN | 03 | 1X3 | 03 | CO | ||
| ο | CN | CN | CN | CN | CN | 0 | «- | «- | 0 | O | O | r- | 1- | ł- | ł- | łr- | t- | »- | O | |||
| CN | 03 | 03 | <0 | CN | cn | O | r- | cX3 | r- | CO | CN | CO | 03 | 1X3 | n- | O | 1X3 | <0 | O) | |||
| Ο | CO | O | O) | r*~ | *— | Ν' | ix) | o> | σ> | T— | 0 | CN | 00 | h- | Ν' | 03 | 1X3 | 3) | ^r | |||
| *t | co | CN | CN | CN | CN | 03 | CN | T- | CN | 03 | CN | 03 | CN | Ί- | CN | T- | 1- | ł- | T- | »- | CN | |
| Ni | id | Z | Z | Z | Z | Z | Νί | |||||||||||||||
| CN | IN | CN | CN | CN | CN | CN | CN | |||||||||||||||
| cn | m | UC3 | in | 1X3 | 1X3 | 1X3 | 1X3 | |||||||||||||||
| CN | CN | CN | CN | CN | CN | CN | CN | |||||||||||||||
| z. | z | Z | Z | Z | Z | Z | Z | |||||||||||||||
| o | O | * | 0 | O | Z | Z | a | 0 | a | 'Z | id | Z | id | id | Ν' | 0 | ||||||
| 03 | oo | CN | ao | CO | CN | CN | CN | co | co | to | CN | CN | CN | CN | CN | CN | CN | CN | CO | |||
| s | Ν | 1x3 | N- | U) | U) | 1X3 | Ν' | 5F | 1X3 | 1X3 | 1X3 | 1X3 | 1X3 | 1X3 | 1X3 | 1X3 | ||||||
| CN | CN | CN | CN | CN | CN | <N | CN | CN | CN | cn | CN | CN | CN | CN | CN | CN | CN | CN | CN | |||
| Z | Z | z | Z | Z | Z | Z | Z | Z | Z | Z | Z | Z | Z | Z | Z | Z | Z | Z | Z | |||
| Ct | Ct | O | QC | a | n | n | 0 | ft | CC | Cd | 0 | n | n | n | Π | a | n | 0 | tt | |||
| cX3 | iX3 | 00 | U3 | m | 00 | co | co | 1X3 | CO | 1X3 | CO | 00 | 00 | CO | co | CO | CO | co | lO | |||
| 3 | Ν’ | N- | Ν’ | •'J | Ν' | Ν’ | Ν' | Ν' | Ν' | Ν' | ’ί | Ν' | Ν' | Tf | ||||||||
| CN | CN | CN | CN | CN | CN | CN | CN | CN | CN | CN | CN | CN | CN | CN | CN | CN | CN | CN | CN | |||
| σ | σ | Z | σ | O | Z | Z | Z | σ | U | σ | Z | Z | Z | Z | Z | z | Z | Z | 0 | |||
| X | X | ar | X | X | et | cd | cd | I | X | X | et | cd | [£ | rr | rr | rr | rr | it | X | |||
| <g | 10 | CD | co | U) | IX) | 1X3 | co | co | co | 1X3 | 1X3 | 1X3 | 1X3 | 1X3 | 1X3 | 1x3 | 1X3 | CD | ||||
| o3 | o5 | Ν’ | 03 | 03 | ''T | Ν' | N | 03 | 03 | O) | <r | T | Ν' | Ν' | Ν' | N | ’Τ | 03 | ||||
| CN | CN | CN | CN | CN | (N | CN | CN | CN | CN | CN | CN | CN | CN | CN | CN | CN | CN | CN | CN | |||
| α | U | O | O | O | O | σ | σ | σ | O | O | O | σ | σ | σ | σ | σ | σ | 0 | 0 | |||
| >- | >- | X | > | X. | > | X | X | X | >- | > | >- | X | X | X | X | X | X | X | et | X | > | |
| CN | CN | CO | CN | CN | CN | co | CX) | co | CN | CN | CN | <0 | co | CD | U) | «) | «) | CO | 1X3 | co | ||
| 03 | 03 | 03 | 03 | IX) | 03 | 03 | 03 | 03 | 03 | 03 | 03 | 03 | 03 | 03 | 03 | O) | O) | O) | Ν' | 03 | 03 | |
| CN | CM | CN | CN | CN | <N | CN | CN | CN | CN | CN | <N | CN | CN | CN | CN | CN | CN | CN | CN | CN | ||
| < | < | a | < | Z | < | U | 0 | 0 | < | < | < | O | O | σ | σ | σ | a | O | 0 | 0 | ||
| O | 0 | > | ct | Cl | cd | > | > | > | Od 03 | Cd | cd | > | > | > | > | > | > | > | X | >- | a. | |
| CN | CN | CN | 03 | co | 03 | CN | CN | CN | 03 | 03 | CN | CN | CN | CN | CN | CN | CN | CN | ||||
| 03 | N- | 03 | 03 | 03 | CN | 03 | 03 | 03 | 03 | 03 | 03 | 03 | 03 | 03 | 03 | |||||||
| CN | CN | <N | CN | CN | CN | CN | p | cn | H | CN | CN | CN | CM | <N | CN | CN | CN | CN | ||||
| w | en | < | H | z | ł- | < | < | ł— | H | < | < | < | < | < | < | < | O | < | < | |||
| Q | o | ft | 0 | Cd | QC | Cl | Cl | O | O | cc | a | Cl | O | O | n | 0 | § | >- | Q_ | a | ||
| cn | c» | cn | 10 | 10 | cn | 03 | CJł | 0 | cn | <33 | cn | 03 | 03 | 03 | O | O | O) | |||||
| cn | cn | cC3 | 3 | ’Τ | 123 | 1x3 | 1X3 | 1X3 | 1X3 | 1X3 | U) | 1X3 | 1X3 | 1X3 | O | O | 03 | O) | 1X3 | |||
| CN | CN | CN | CN | CN | ||||||||||||||||||
| 0 | 0 | H | 0 | 0 | U | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | en | > | 0. | < | 0 | ||
| 0 | er | >- | >- | 0 | 0 | 0 | _J | 0 | _l | > | ci | Cl | Cl | O | a | Q | ||||||
| 't | 3- | cn | 03 | CN | 0 | n | 0 | CD | CD | 5 | CO | CO | cn | cn | O) | O) | O) | O) | ||||
| o | 0 | U3 | O | 03 | 03 | 03 | 03 | CN | CN | 0 | CN | CN | 03 | 0 | 0 | IX) | U) | «) | uf» | 1X3 | 1X3 | P |
| CN | ||||||||||||||||||||||
| > | > | 0 | σ | < | < | cn | en | en | CO | > | CO | CO | CŁ | > | > | 0 | 0 | 0 | 0 | 0 | 0 | > |
| < | < | Cl | 0 | < | < | <r | < | |||||||||||||||
| 03 O | 03 O | 3 | Ν’ σ | 03 cX3 | iX3 | ’Τ o | »T 0 | Ν' o | 3 | 03 O | Ν' O | 3 | 3 | 03 O | 03 O | 3 | 3 | 3 | 3 | 3 | «ί- ο | 03 O |
| W | W | > | > | 0 | 0 | > | > | > | > | CO | > | > | > | (0 | <n | > | > | > | > | > | > | cn |
| s | < CN | s | s | s | $ | s | $ | 0 | < 03 | < 03 | S | 0 | 0 | $ | s | < 03 | < 03 | < 03 | < 03 | 0 | ||
| O | 0 | 0 | O | O | 0 | 0 | 0 | 0 | 0 | O | O | 0 | O | o | 0 | 0 | O | O | O | O | 0 | |
| 0 | 0 | W | w | > | > | cn | en | cn | en | en | CO | en | <0 | en | en | cn | en | en | cn | en | cn | en |
| _l | 0 | s | 0 | s | s | O | 0 | 0 | 0 | O | 0 | a | 0 | > | 0 | 0 | 0 | 0 | 0 | 0 | 0 | O |
| 00 | O | 0 | CN | 0 | 0 | CN | 0 | 0 | 0 | CN | CN | CN | 0 | co | 03 | 0 | 0 | 0 | 0 | 0 | 0 | CO |
| cn | CD | to | CI3 | CX3 | CO | 03 | O) | h- | ||||||||||||||
| < | en | 0 | Z | w | cn | Z | w | w | co | Z | Z | Z | <0 | < | en | cn | cn | cn | cn | cn | « | Z |
PL 191 529 B1
Lista sekwencji <110> Poulose, Ayrookaran J.
Schellenberger, Volker Kellis, Jr,, James T.
Paech, Christian Nadherny, Joannę Naki, Donald P.
<120> Nowa odmiana proteazy, cząsteczka DNA kodująca nową odmianę proteazy, wektor zawierający tę cząsteczkę oraz karma dla zwierząt i kompozycje zawierające nową odmianę proteazy <130> GC502-2 <140> 09/178,155 <141> 1998-10-23 <150> 08/956,323 <151> 1997-10-23 <150> 08/956,564 <151> 1997-10-23 <150> 08/956,324 <151> 1997-10-23 <160> 6 <170> FastSEO do Windows Version 3.0 <210> 1 <211> 1497 <212> DNA <213> B. amyloliąuefaciens <220>
<221> CDS <222> (96) ... (1245) <400> 1 ggtctactaa aatattattc catactatac aattaataca cagaataatc tgtctattgg 60 ttattctgca aatgaaaaaa aggagaggat aaaga gtg aga ggc aaa aaa gta 113
Met Arg Gly Lys Lys Val 1 5
| tgg | atc | agt | ttg | ctg | ttt | gct | tta | gcg | tta | atc | ttt | acg | atg | gcg | ttc |
| 161 | |||||||||||||||
| Trp | Ile | Ser | Leu 10 | Leu | Phe | Ala | Leu | Ala 15 | Leu | Tle | Phe | Thr | Met 20 | Ala | Phe |
| ggc | agc | aca | tcc | tct | gcc | eag | gcg | gca | ggg | aaa | tca | aac | ggg | gaa | aag |
| 209 | |||||||||||||||
| Gly | Ser | Thr 25 | Ser | Ser | Ala | Gin | Ala 30 | Ala | Gly | Lys | Ser | Asn 35 | Gly | Glu | Lys |
| aaa | tat | att | gtc | ggg | ttt | aaa | cag | aca | atg | agc | acg | atg | agc | gcc | gct |
| 257 | |||||||||||||||
| Lys | Tyr | Ile | Val | Gly | Phe | Lys | Gin | Thr | Met | Ser | Thr | Met | Ser | Ala | Ala |
PL 191 529 B1
| 40 | 45 | 50 | |||||||||||||
| aag | aag | aaa | gat | gtc | att | tct | gaa | aaa | ggc | ggg | aaa | gtg | caa | aag | caa |
| 305 | |||||||||||||||
| Lys | Lys | Lys | Asp | Val | Ile | Ser | Glu | Lys | Gly | Gly | Lys | Val | Gin | Lys | Gin |
| 55 | 60 | 65 | 70 | ||||||||||||
| ttc | aaa | tat | gta | gac | gca | gct | tca | gtc | aca | tta | aac | gaa | aaa | gct | gta |
| 353 | |||||||||||||||
| Phe | Lys | Tyr | Val | Asp | Ala | Ala | Ser | val | Thr | Leu | Asn | Glu | Lys | Ala | Val |
| 75 | 80 | 85 | |||||||||||||
| aaa | gaa | ttg | aaa | aaa | gac | ccg | agc | gtc | gct | tac | gtt | gaa | gaa | gat | cac |
| 401 | |||||||||||||||
| Lys | Glu | Leu | Lys | Lys | Asp | Pro | Ser | Val | Ala | Tyr | Val | Glu | Glu | Asp | His |
| 90 | 95 | 100 | |||||||||||||
| gta | gca | cat | gcg | tac | gcg | cag | tcc | gtg | cct | tac | ggc | gta | tca | caa | att |
| 449 | |||||||||||||||
| Val | Ala | His | Ala | Tyr | Ala | Gin | Ser | Val | Pro | Tyr | Gly | Val | Ser | Gin | Ile |
| 105 | 110 | 115 | |||||||||||||
| aaa | gcc | cct | gct | ctg | cac | tct | caa | ggc | tac | act | gga | tca | aat | gtt | aaa |
| 4 97 | |||||||||||||||
| Lys | Ala | Pro | Ala | Leu | His | Ser | Gin | Gly | Tyr | Thr | Gly | Ser | Asn | Val | Lys |
| 120 | 125 | 130 | |||||||||||||
| gta | gcg | gtt | atc | gac | agc | ggt | atc | gat | tct | tct | cat | cct | gat | tta | aag |
| 545 | |||||||||||||||
| Val | Ala | Val | Ile | Asp | Ser | Gly | Ile | Asp | Ser | Ser | His | Pro | Asp | Leu | Lys |
| 135 | 140 | 145 | 150 | ||||||||||||
| gta | gca | agc | gga | gcc | agc | atg | gtt | cct | tct | gaa | aca | aat | cct | ttc | caa |
| 593 | |||||||||||||||
| Val | Ala | Ser | Gly | Ala | Ser | Met | Val | Pro | Ser | Glu | Thr | Asn | Pro | Phe | Gin |
| 155 | 160 | 165 | |||||||||||||
| gac | aac | aac | tct | cac | gga | act | cac | gtt | gcc | ggc | aca | gtt | gcg | gct | ctt |
| 641 | |||||||||||||||
| Asp | Asn | Asn | Ser | His | Gly | Thr | His | Val | Ala | Gly | Thr | Val | Ala | Ala | Leu |
| 170 | 175 | 180 | |||||||||||||
| aat | aac | tca | atc | ggt | gta | tta | ggc | gtt | gcg | cca | agc | gca | tca | ctt | tac |
| 689 | |||||||||||||||
| Asn | Asn | Ser | Ile | Gly | Val | Leu | Gly | Val | Ala | Pro | Ser | Ala | Ser | Leu | Tyr |
| 185 | 190 | 195 | |||||||||||||
| gct | gta | aaa | gtt | ctc | ggt | gct | gac | ggt | tcc | ggc | caa | tac | agc | tgg | atc |
| 737 | |||||||||||||||
| Ala | Val | Lys | Val | Leu | Gly | Ala | Asp | Gly | Ser | Gly | Gin | Tyr | Ser | Trp | Ile |
| 200 | 205 | 210 | |||||||||||||
| att | aac | gga | atc | gag | tgg | gcg | atc | gca | aac | aat | atg | gac | gtt | att | aac |
| 785 | |||||||||||||||
| Ile | Asn | Gly | Ile | Glu | Trp | Ala | Ile | Ala | Asn | Asn | Met | Asp | Val | Ile | Asn |
| 215 | 220 | 225 | 230 | ||||||||||||
| atg | agc | ctc | ggc | gga | cct | tct | ggt | tct | gct | gct | tta | aaa | gcg | gca | gtt |
| 833 | |||||||||||||||
| Met | Ser | Leu | Gly | Gly | Pro | Ser | dy | Ser | Ala | Ala | Leu | Lys | Ala | Ala | Val |
PL 191 529 B1
| 235 | 240 | 245 | |
| gat aaa gcc gtt | gca tcc ggc gtc gta | gtc gtt gcg | gca gcc ggt aac |
| 881 | |||
| Asp Lys Ala Val | Ala Ser Gly Val Val | Val Val Ala | Ala Ala Gly Asn |
| 250 | 255 | 260 | |
| gaa ggc act tcc | ggc agc tca agc aca | gtg ggc tac | cct ggt aaa tac |
| 929 | |||
| Glu Gly Thr Ser | Gly Ser Ser Ser Thr | Val Gly Tyr | Pro Gly Lys Tyr |
| 265 | 270 | 275 | |
| cct tct gtc att | gca gta ggc gct gtt | gac agc agc | aac caa aga gca |
| 977 | |||
| Pro Ser Val Ile | Ala Val Gly Ala Val | Asp Ser Ser | Asn Gin Arg Ala |
| 280 | 285 | 290 | |
| tct ttc tca agc | gta gga cct gag ctt | gat gtc atg | gca cct ggc gta |
| 1025 | |||
| Ser Phe Ser Ser | Val Gly Pro Glu Leu | Asp Val Met | Ala Pro Gly Val |
| 295 | 300 | 305 | 310 |
| tct atc caa agc | acg ctt cct gga aac | aaa tac ggg | gcg tac aac ggt |
| 1073 | |||
| Ser Ile Gin Ser | Thr Leu Pro Gly Asn | Lys Tyr Gly | Ala Tyr Asn Gly |
| 315 | 320 | 325 | |
| acg tca atg gca | tct ccg cac gtt gcc | gga gcg gct | gct ttg att ctt |
| 1121 | |||
| Thr Ser Met Ala | Ser Pro His Val Ala | Gly Ala Ala | Ala Leu Ile Leu |
| 330 | 335 | 340 | |
| tct aag cac ccg | aac tgg aca aac act | caa gtc cgc | agc agt tta gaa |
| 1169 | |||
| Ser Lys His Pro | Asn Trp Thr Asn Thr | Gin Val Arg | Ser Ser Leu Glu |
| 345 | 350 | 355 | |
| aac acc act aca | aaa ctt ggt gat tct | ttg tac tat | gga aaa ggg ctg |
| 1217 | |||
| Asn Thr Thr Thr | Lys Leu Gly Asp Ser | Leu Tyr Tyr | Gly Lys Gly Leu |
| 360 | 365 | 370 | |
| atc aac gta caa | gcg gca gct cag taa | a acataaaaaa ccggccttgg | |
| 1265 | |||
| Ile Asn Val Gin | Ala Ala Ala Gin * | ||
| 375 | 380 |
ccccgccggt
1325 gatggctccc
1385 cggccaactc
1445 aacggtcggc
1497 tttttattat tctgaaaatt ctgaaacgtc ggcgttttcc ttttcttcct ttaacgagaa tcaatcgccg tgataccggg ccgcatgttc acggcgggtt cttcccggtt agacggcatt aatccgctcc gacccggctc tccggtcagc cgtaatcgga ataatcgacg agtcccgtaa tcaatgccat tc <210> 2 <211> 382 <212> PRT <213> B. amyloliąuefaciens
PL 191 529 B1 <400> 2
| Met | Arg | Gly | Lys | Lys | Val | Trp | Ile | Ser | Leu | Leu | Phe | Ala | Leu | Ala | Leu |
| 1 | 5 | 10 | 15 | ||||||||||||
| Ile | Phe | Thr | Met | Ala | Phe | Gly | Ser | Thr | Ser | Ser | Ala | Gin | Ala | Ala | Gly |
| 20 | 25 | 30 | |||||||||||||
| Lys | Ser | Asn | Gly | Glu | Lys | Lys | Tyr | Ile | Val | Gly | Phe | Lys | Gin | Thr | Met |
| 35 | 40 | 45 | |||||||||||||
| Ser | Thr | Met | Ser | Ala | Ala | Lys | Lys | Lys | Asp | Val | Ile | Ser | Glu | Lys | Gly |
| 50 | 55 | 60 | |||||||||||||
| Gly | Lys | Val | Gin | Lys | Gin | Phe | Lys | Tyr | Val | Asp | Ala | Ala | Ser | Val | Thr |
| 65 | 70 | 75 | 80 | ||||||||||||
| Leu | Asn | Glu | Lys | Ala | Val | Lys | Glu | Leu | Lys | Lys | Asp | Pro | Ser | Val | Ala |
| 85 | 90 | 95 | |||||||||||||
| Tyr | Val | Glu | Glu | Asp | His | Val | Ala | His | Ala | Tyr | Ala | Gin | Ser | Val | Pro |
| 100 | 105 | 110 | |||||||||||||
| Tyr | Gly | Val | Ser | Gin | Ile | Lys | Ala | Pro | Ala | Leu | His | Ser | Gin | Gly | Tyr |
| 115 | 120 | 125 | |||||||||||||
| Thr | Gly | Ser | Asn | Val | Lys | Val | Ala | Val | Ile | Asp | Ser | Gly | Ile | Asp | Ser |
| 130 | 135 | 140 | |||||||||||||
| Ser | His | Pro | Asp | Leu | Lys | Val | Ala | Ser | Gly | Ala | Ser | Met | Val | Pro | Ser |
| 145 | 150 | 155 | 160 | ||||||||||||
| Glu | Thr | Asn | Pro | Phe | Gin | Asp | Asn | Asn | Ser | His | Gly | Thr | His | Val | Ala |
| 165 | 170 | 175 | |||||||||||||
| Gly | Thr | Val | Ala | Ala | Leu | Asn | Asn | Ser | Ile | Gly | Val | Leu | Gly | Val | Ala |
| 180 | 185 | 190 | |||||||||||||
| Pro | Ser | Ala | Ser | Leu | Tyr | Ala | Val | Lys | Val | Leu | Gly | Ala | Asp | Gly | Ser |
| 195 | 200 | 205 | |||||||||||||
| Gly | Gin | Tyr | Ser | Trp | Ile | Ile | Asn | Gly | Ile | Glu | Trp | Ala | Ile | Ala | Asn |
| 210 | 215 | 220 | |||||||||||||
| Asn | Met | Asp | Val | Ile | Asn | Met | Ser | Leu | Gly | Gly | Pro | Ser | Gly | Ser | Ala |
| 225 | 230 | 235 | 240 | ||||||||||||
| Ala | Leu | Lys | Ala | Ala | Val | Asp | Lys | Ala | Val | Ala | Ser | Gly | Val | Val | Val |
| 245 | 250 | 255 | |||||||||||||
| Val | Ala | Ala | Ala | Gly | Asn | Glu | Gly | Thr | Ser | Gly | Ser | Ser | Ser | Thr | Val |
| 260 | 265 | 270 | |||||||||||||
| Gly | Tyr | Pro | Gly | Lys | Tyr | Pro | Ser | Val | Ile | Ala | Val | Gly | Ala | Val | Asp |
| 275 | 280 | 285 | |||||||||||||
| Ser | Ser | Asn | Gin | Arg | Ala | Ser | Phe | Ser | Ser | Val | Gly | Pro | Glu | Leu | Asp |
| 290 | 295 | 300 | |||||||||||||
| Val | Met | Ala | Pro | Gly | Val | Ser | Ile | Gin | Ser | Thr | Leu | Pro | Gly | Asn | Lys |
| 305 | 310 | 315 | 320 | ||||||||||||
| Tyr | Gly | Ala | Tyr | Asn | Gly | Thr | Ser | Met | Ala | Ser | Pro | His | Val | Ala | Gly |
| 325 | 330 | 335 | |||||||||||||
| Ala | Ala | Ala | Leu | Ile | Leu | Ser | Lys | His | Pro | Asn | Trp | Thr | Asn | Thr | Gin |
| 340 | 345 | 350 | |||||||||||||
| Val | Arg | Ser | Ser | Leu | Glu | Asn | Thr | Thr | Thr | Lys | Leu | Gly | Asp | Ser | Leu |
| 355 | 360 | 365 | |||||||||||||
| Tyr | Tyr | Gly | Lys | Gly | Leu | Ile | Asn | Val | Gin | Ala | Ala | Ala | Gin | ||
| 370 | 375 | 380 | |||||||||||||
| <210> | 3 | ||||||||||||||
| <211> | 275 | ||||||||||||||
| <212> | PRT | ||||||||||||||
| <213> | B. amyloliąuefaciens | ||||||||||||||
| <400> | 3 | ||||||||||||||
| Ala | Gin | Ser | Val | Pro | Tyr | Gly | Val | Ser | Gin | Ile | Lys | Ala | Pro | Ala | Leu |
| 1 | 5 | 10 | 15 | ||||||||||||
| His | Ser | Gin | Gly | Tyr | Thr | Gly | Ser | Asn | Val | Lys | Val | Ala | Val | Ile | Asp |
| 20 | 25. | 30 |
PL 191 529 B1
| Ser | Gly | Ile Asp 35 | Ser | Ser His | Pro Asp Leu Lys Val Ala | Gly | Gly | Ala | |||||||
| 40 | 45 | ||||||||||||||
| Ser | Met | Val | Pro | Ser | Glu | Thr | Asn | Pro | Phe | Gin | Asp | Asn | Asn | Ser | His |
| 50 | 55 | 60 | |||||||||||||
| Gly | Thr | His | Val | Ala | Gly | Thr | Val | Ala | Ala | Leu | Asn | Asn | Ser | Ile | Gly |
| 65 | 70 | 75 | 80 | ||||||||||||
| Val | Leu | Gly | Val | Ala | Pro | Ser | Ala | Ser | Leu | Tyr | Ala | val | Lys | Val | Leu |
| 85 | 90 | 95 | |||||||||||||
| Gly | Ala | Asp | Gly | Ser | Gly | Gin | Tyr | Ser | Trp | Ile | Ile | Asn | Gly | Ile | Glu |
| 100 | 105 | 110 | |||||||||||||
| Trp | Ala | Ile | Ala | Asn | Asn | Met | Asp | Val | Ile | Asn | Met | Ser | Leu | Gly | Gly |
| 115 | 120 | 125 | |||||||||||||
| Pro | Ser | Gly | Ser | Ala | Ala | Leu | Lys | Ala | Ala | Val | Asp | Lys | Ala | Val | Ala |
| 130 | 135 | 140 | |||||||||||||
| Ser | Gly | Val | Val | Val | Val | Ala | Ala | Ala | Gly | Asn | Glu | Gly | Thr | Ser | Gly |
| 145 | 150 | 155 | 160 | ||||||||||||
| Ser | Ser | Ser | Thr | Val | Gly | Tyr | Pro | Gly | Lys | Tyr | Pro | Ser | Val | Ile | Ala |
| 165 | 170 | 175 | |||||||||||||
| Val | Gly | Ala | Val | Asp | Ser | Ser | Asn | Gin | Arg | Ala | Ser | Phe | Ser | Ser | Val |
| 180 | 185 | 190 | |||||||||||||
| Gly | Pro | Glu | Leu | Asp | Val | Met | Ala | Pro | Gly | Val | Ser | Ile | Gin | Ser | Thr |
| 195 | 200 | 205 | |||||||||||||
| Leu | Pro | Gly | Asn | Lys | Tyr | Gly | Ala | Tyr | Asn | Gly | Thr | Ser | Met | Ala | Ser |
| 210 | 215 | 220 | |||||||||||||
| Pro | His | Val | Ala | Gly | Ala | Ala | Ala | Leu | Ile | Leu | Ser | Lys | His | Pro | Asn |
| 225 | 230 | 235 | 240 | ||||||||||||
| Trp | Thr | Asn | Thr | Gin | Val | Arg | Ser | Ser | Leu | Glu | Asn | Thr | Thr | Thr | Lys |
| 245 | 250 | 255 | |||||||||||||
| Leu | Gly | Asp | Ser | Phe | Tyr | Tyr | Gly | Lys | Gly | Leu | Ile | Asn | Val | Gin | Ala |
| 260 | 265 | 270 | |||||||||||||
| Ala | Ala | Gin | |||||||||||||
| 275 | |||||||||||||||
| <210> | 4 | ||||||||||||||
| <211> | 275 | ||||||||||||||
| <212> | PRT | ||||||||||||||
| <213> | B. subtilis | ||||||||||||||
| <400> | 4 | ||||||||||||||
| Ala | Gin | Ser | Val | Pro | Tyr | Gly | Ile | Ser | Gin | Ile | Lys | Ala | Pro | Ala | Leu |
| 1 | 5 | 10 | 15 | ||||||||||||
| His | Ser | Gin | Gly | Tyr | Thr | Gly | Ser | Asn | Val | Lys | Val | Ala | Val | Ile | Asp |
| 20 | 25 | 30 | |||||||||||||
| Ser | Gly | Ile | Asp | Ser | Ser | His | Pro | Asp | Leu | Asn | Val | Arg | Gly | Gly | Ala |
| 35 | 40 | 45 | |||||||||||||
| Ser | Phe | val | Pro | Ser | Glu | Thr | Asn | Pro | Tyr | Gin | Asp | Gly | Ser | Ser | His |
| 50 | 55 | 60 | |||||||||||||
| Gly | Thr | His | Val | Ala | Gly | Thr | Ile | Ala | Ala | Leu | Asn | Asn | Ser | Ile | Gly |
| 65 | 70 | 75 | 80 | ||||||||||||
| Val | Leu | Gly | Val | Ser | Pro | Ser | Ala | Ser | Leu | Tyr | Ala | Val | Lys | val | Leu |
| 85 | 90 | 95 | |||||||||||||
| Asp | Ser | Thr | Gly | Ser | Gly | Gin | Tyr | Ser | Trp | Ile | Ile | Asn | Gly | Ile | Glu |
| 100 | 105 | 110 | |||||||||||||
| Trp | Ala | Ile | Ser | Asn | Asn | Met | Asp | Val | ile | Asn | Met | Ser | Leu | Gly | Gly |
| 115 | 120 | 125 | |||||||||||||
| Pro | Thr | Gly | Ser | Thr | Ala | Leu | Lys | Thr | Val | Val | Asp | Lys | Ala | Val | Ser |
| 130 | 135 | 140 | |||||||||||||
| Ser | Gly | Ile | Val | Val | Ala | Ala | Ala | Ala | Gly | Asn | Glu | Gly | Ser | Ser | Gly |
| 145 | 150 | 155 | 160 | ||||||||||||
| Ser | Thr | Ser | Thr | Val | Gly | Tyr | Pro | Ala | Lys | Tyr | Pro | Ser | Thr | Ile | Ala |
PL 191 529 B1
| 165 | 170 | 175 | |
| Val | Gly Ala Val Asn Ser Ser Asn 180 | Gin Arg Ala Ser Phe Ser 185 190 | Ser Ala |
| Gly | Ser Glu Leu Asp Val Met Ala 195 200 | Pro Gly Val Ser Ile Gin 205 | Ser Thr |
| Leu | Pro Gly Gly Thr Tyr Gly Ala 210 215 | Tyr Asn Gly Thr Ser Met 220 | Ala Thr |
| Pro 225 | His Val Ala Gly Ala Ala Ala 230 | Leu Ile Leu Ser Lys His 235 | Pro Thr 240 |
| Trp | Thr Asn Ala Gin Val Arg Asp 245 | Arg Leu Glu Ser Thr Ala 250 | Thr Tyr 255 |
| Leu Ala | Gly Asn Ser Phe Tyr Tyr Gly 260 Ala Gin 275 <210> 5 <211> 274 <212> PRT <213> B. licheniformis <400> 5 | Lys Gly Leu Ile Asn Val 265 270 | Gin Ala |
| Ala 1 | Gin Thr Val Pro Tyr Gly Ile 5 | Pro Leu Ile Lys Ala Asp 10 | Lys Val 15 |
| Gin | Ala Gin Gly Phe Lys Gly Ala 20 | Asn Val Lys Val Ala Val 25 30 | Leu Asp |
| Thr | Gly Ile Gin Ala Ser His Pro 35 40 | Asp Leu Asn Val Val Gly 45 | Gly Ala |
| Ser | Phe Val Ala Gly Glu Ala Tyr 50 55 | Asn Thr Asp Gly Asn Gly 60 | His Gly |
| Thr 65 | His Val Ala Gly Thr Val Ala 70 | Ala Leu Asp Asn Thr Thr 75 | Gly Val 80 |
| Leu | Gly Val Ala Pro Ser Val Ser 85 | Leu Tyr Ala Val Lys Val 90 | Leu Asn 95 |
| Ser | Ser Gly Ser Gly Ser Tyr Ser 100 | Gly Ile Val Ser Gly Ile 105 110 | Glu Trp |
| Ala | Thr Thr Asn Gly Met Asp Val 115 120 | Ile Asn Met Ser Leu Gly 125 | Gly Ala |
| Ser | Gly Ser Thr Ala Met Lys Gin 130 135 | Ala Val Asp Asn Ala Tyr 140 | Ala Arg |
| Gly 145 | Val Val Val Val Ala Ala Ala 150 | Gly Asn Ser Gly Asn Ser 155 | Gly Ser 160 |
| Thr | Asn Thr Ile Gly Tyr Pro Ala 165 | Lys Tyr Asp Ser Val Ile 170 | Ala Val 175 |
| Gly | Ala Val Asp Ser Asn Ser Asn 180 | Arg Ala Ser Phe Ser Ser 185 190 | Val Gly |
| Ala | Glu Leu Glu Val Met Ala Pro 195 200 | Gly Ala Gly Val Tyr Ser 205 | Thr Tyr |
| Pro | Thr Asn Thr Tyr Ala Thr Leu 210 215 | Asn Gly Thr Ser Met Ala 220 | Ser Pro |
| His 225 | Val Ala Gly Ala Ala Ala Leu 230 | Ile Leu Ser Lys His Pro 235 | Asn Leu 240 |
| Ser | Ala Ser Gin Val Arg Asn Arg 245 | Leu Ser Ser Thr Ala Thr 250 | Tyr Leu 255 |
| Gly Ala | Ser Ser Phe Tyr Tyr Gly Lys 260 Gin <210> 6 <211> 269 <212> PRT | Gly Leu Ile Asn Val Glu 265 270 | Ala Ala |
PL 191 529 B1 <213> Β. lentus <400> 6
| Ala | Gin | Ser | Val | Pro | Trp | Gly | Ile | Ser | Arg | Val | Gin | Ala | Pro | Ala | Ala |
| 1 | 5 | 10 | 15 | ||||||||||||
| His | Asn | Arg | Gly | Leu | Thr | Gly | Ser | Gly | Val | Lys | Val | Ala | Val | Leu | Asp |
| 20 | 25 | 30 | |||||||||||||
| Thr | Gly | Ile | Ser | Thr | His | Pro | Asp | Leu | Asn | Ile | Arg | Gly | Gly | Ala | Ser |
| 35 | 40 | 45 | |||||||||||||
| Phe | Val | Pro | Gly | Glu | Pro | Ser | Thr | Gin | Asp | Gly | Asn | Gly | His | Gly | Thr |
| 50 | 55 | 60 | |||||||||||||
| His | Val | Ala | Gly | Thr | Ile | Ala | Ala | Leu | Asn | Asn | Ser | Ile | Gly | Val | Leu |
| 65 | 70 | 75 | 80 | ||||||||||||
| Gly | Val | Ala | Pro | Ser | Ala | Glu | Leu | Tyr | Ala | Val | Lys | Val | Leu | Gly | Ala |
| 85 | 90 | 95 | |||||||||||||
| Ser | Gly | Ser | Gly | Ser | Val | Ser | Ser | Ile | Ala | Gin | Gly | Leu | Glu | Trp | Ala |
| 100 | 105 | 110 | |||||||||||||
| Gly | Asn | Asn | Gly | Met | His | Val | Ala | Asn | Leu | Ser | Leu | Gly | Ser | Pro | Ser |
| 115 | 120 | 125 | |||||||||||||
| Pro | Ser | Ala | Thr | Leu | Glu | Gin | Ala | Val | Asn | Ser | Ala | Thr | Ser | Arg | Gly |
| 130 | 135 | 140 | |||||||||||||
| Val | Leu | Val | Val | Ala | Ala | Ser | Gly | Asn | Ser | Gly | Ala | Gly | Ser | Ile | Ser |
| 145 | 150 | 155 | 160 | ||||||||||||
| Tyr | Pro | Ala | Arg | Tyr | Ala | Asn | Ala | Met | Ala | Val | Gly | Ala | Thr | Asp | Gin |
| 165 | 170 | 175 | |||||||||||||
| Asn | Asn | Asn | Arg | Ala | Ser | Phe | Ser | Gin | Tyr | Gly | Ala | Gly | Leu | Asp | Ile |
| 180 | 185 | 190 | |||||||||||||
| Val | Ala | Pro | Gly | Val | Asn | Val | Gin | Ser | Thr | Tyr | Pro | Gly | Ser | Thr | Tyr |
| 195 | 200 | 205 | |||||||||||||
| Ala | Ser | Leu | Asn | Gly | Thr | Ser | Met | Ala | Thr | Pro | His | Val | Ala | Gly | Ala |
| 210 | 215 | 220 | |||||||||||||
| Ala | Ala | Leu | Val | Lys | Gin | Lys | Asn | Pro | Ser | Trp | Ser | Asn | Val | Gin | Ile |
| 225 | 230 | 235 | 240 | ||||||||||||
| Arg | Asn | His | Leu | Lys | Asn | Thr | Ala | Thr | Ser | Leu | Gly | Ser | Thr | Asn | Leu |
| 245 | 250 | 255 | |||||||||||||
| Tyr | Gly | Ser | Gly | Leu | Val | Asn | Ala | Glu | Ala | Ala | Thr | Arg | |||
| 260 | 265 |
Zastrzeżenia patentowe
Claims (16)
1.Odmiana proteazy, znamienna tym, że zawiera podstawienie aminowasowe w pozycji reszty odpowiadającej pozycji 103 oraz przynajmniej jednej z pozycji 236 i 245 w subtylizynie z Bacillus amyloliquefaciens oraz podstawienie przynajmniej jednego aminokwasu w pozycji reszty odpowiadającej następującym pozycjom w tej subtylizynie: 1, 3, 4, 8, 10, 12, 13, 16, 17, 18, 19, 20, 21, 22, 24, 27, 33,
37, 38, 42, 43, 48, 55, 57, 58, 61, 62, 68, 72, 75, 76, 77, 78, 79, 86, 87, 89, 97, 98, 99, 101, 102, 104, 106, 107, 109, 111, 114, 116, 117, 119, 121, 123, 126, 128, 130, 131, 133, 134, 137, 140, 141, 142,
146, 147, 158, 159, 160, 166, 167, 170, 173, 174, 177,181, 182, 183, 184, 185, 188, 192, 194, 198,
203, 204, 205, 206, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 222, 224, 227, 228, 230, 232,
237, 238, 240, 242, 243, 244, 245, 246, 247, 248, 249, 251, 252, 253, 254, 255, 256, 257, 258, 259,
260, 261, 262, 263, 265, 268, 269, 270, 271, 272, 274 oraz 275, przy czym jeśli odmiana obejmuje podstawienie jednocześnie w pozycjach 103 i 76, to występuje również podstawienie w przynajmniej jednej z pozycji innych niż odpowiadające następującym pozycjom 27, 99, 101, 104, 107, 109, 123, 128, 166, 204, 206, 210, 216, 217, 218, 222, 260, 265 lub 274.
2.Odmiana proteazy według zastrz. 1, znamienna tym, że zawiera podstawienie aminowasowe w pozycji reszty odpowiadającej pozycji 103 i 236 oraz w przynajmniej jednej z pozycji: 1, 9, 12,
61, 62, 68, 76, 97, 98, 97, 98, 101, 102, 104, 109, 130, 131, 159, 183, 185, 205, 209, 210, 211, 212, 213, 215, 217, 230, 232, 248, 252, 257, 260, 270 i 275.
3.Odmiana proteazy według zastrz. 1, znamienna tym, że zawiera podstawienie aminowasowe w pozycji reszty odpowiadającej pozycji 103 i 245 oraz w przynajmniej jednej z pozycji: 1, 9, 12,
PL 191 529 B1
61, 62, 68, 76, 97, 98, 97, 98, 101, 102, 104, 109, 130, 131, 159, 183, 185, 205, 209, 210, 211, 212, 213, 215, 217, 230, 232, 248, 252, 257, 260, 270 i 275.
4. Odmiana proteazy według zastrz. 1, znamienna tym, że zawiera podstawienie aminowasowe w pozycji reszty odpowiadającej pozycji 103, 236 i 245 oraz w przynajmniej jednej z pozycji: 1, 9, 12, 61, 62, 68, 76, 97, 98, 97, 98, 101, 102, 104, 109, 130, 131, 159, 183, 185, 205, 209, 210, 211, 212, 213, 215, 217, 230, 232, 248, 252, 257, 260, 270 i 275.
5. Odmiana proteazy według zastrz. 1, albo 2, albo 3, albo 4, znamienna tym, że zawiera podstawienie aminowasowe w pozycji reszty odpowiadającej pozycji 159.
6. Odmiana proteazy według zastrz. 1, znamienna tym, że pochodzi od subtylizyny z Bacillus.
7. Odmiana proteazy według zastrz. 6, znamienna tym, że pochodzi od subtylizyny z Bacillus lentus.
8. Cząsteczka DNA, znamienna tym, że zawiera kodony kodujące odmianę proteazy, która zawiera podstawienie aminowasowe w pozycji reszty odpowiadającej pozycji 103 oraz przynajmniej jednej z pozycji 236 i 245 w subtylizynie z Bacillus amyloliquefaciens o sekwencji nr 1 oraz podstawienie przynajmniej jednego aminokwasu w pozycji reszty odpowiadającej następującym pozycjom w tej subtylizynie: 1, 3, 4, 8, 10, 12, 13, 16, 17, 18, 19, 20, 21, 22, 24, 27, 33, 37, 38, 42, 43, 48, 55, 57, 58, 61, 62, 68, 72, 75, 76, 77, 78, 79, 86, 87, 89, 97, 98, 99, 101, 102, 104, 106, 107, 109, 111, 114, 116, 117, 119, 121, 123, 126, 128, 130, 131, 133, 134, 137, 140, 141, 142, 146, 147, 158, 159, 160, 166, 167, 170, 173, 174, 177,181, 182, 183, 184, 185, 188, 192, 194, 198, 203, 204, 205, 206, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 222, 224, 227, 228, 230, 232, 237, 238, 240, 242, 243, 244, 245, 246, 247, 248, 249, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 265, 268, 269, 270, 271, 272, 274 oraz 275, przy czym jeśli odmiana obejmuje podstawienie jednocześnie w pozycjach 103 i 76, to występuje również podstawienie w przynajmniej jednej z pozycji innych niż odpowiadające następującym pozycjom 27, 99, 101, 104, 107, 109, 123, 128, 166, 204, 206, 210, 216, 217, 218, 222, 260, 265 lub 274.
9. Wektor ekspresyjny, znamienny tym, że obejmuje sekwencje regulatorowe funkcjonalnie połączone z sekwencją DNA kodującą odmianę proteazy, która zawiera podstawienie aminowasowe w pozycji reszty odpowiadającej pozycji 103 oraz przynajmniej jednej z pozycji 236 i 245 w subtylizynie z Bacillus amyloliquefaciens o sekwencji nr 1 oraz podstawienie przynajmniej jednego aminokwasu w pozycji reszty odpowiadającej następującym pozycjom w tej subtylizynie: 1, 3, 4, 8, 10, 12, 13, 16, 17, 18, 19, 20, 21, 22, 24, 27, 33, 37, 38, 42, 43, 48, 55, 57, 58, 61, 62, 68, 72, 75, 76, 77, 78, 79, 86, 87, 89, 97, 98, 99, 101, 102, 104, 106, 107, 109, 111, 114, 116, 117, 119, 121, 123, 126, 128, 130, 131, 133, 134, 137, 140, 141, 142, 146, 147, 158, 159, 160, 166, 167, 170, 173, 174, 177,181, 182, 183, 184, 185, 188, 192, 194, 198, 203, 204, 205, 206, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 222, 224, 227, 228, 230, 232, 237, 238, 240, 242, 243, 244, 245, 246, 247, 248, 249, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 265, 268, 269, 270, 271, 272, 274 oraz 275, przy czym jeśli odmiana obejmuje podstawienie jednocześnie w pozycjach 103 i 76, to występuje również podstawienie w przynajmniej jednej z pozycji innych niż odpowiadające następującym pozycjom 27, 99, 101, 104, 107, 109, 123, 128, 166, 204, 206, 210, 216, 217, 218, 222, 260, 265 lub 274, korzystnie sekwencja regulatorowa obejmuje promotor, sekwencje operatora, sekwencję kodującą miejsce wiązania rybosomu z mRNA oraz sekwencje regulujące terminację transkrypcji i translacji.
10. Komórka gospodarza, znamienna tym, że jest transforomowana wektorem ekspresyjnym obejmującym sekwencje regulatorowe funcjonalnie połączone z sekwencją DNA kodującą odmianę proteazy, która zawiera podstawienie aminowasowe w pozycji reszty odpowiadającej pozycji 103 oraz przynajmniej jednej z pozycji 236 i 245 w subtylizynie z Bacillus amyloliquefaciens o sekwencji nr 1 oraz podstawienie przynajmniej jednego aminokwasu w pozycji reszty odpowiadającej następującym pozycjom w tej subtylizynie: 1, 3, 4, 8, 10, 12, 13, 16, 17, 18, 19, 20, 21, 22, 24, 27, 33, 37, 38, 42, 43, 48, 55, 57, 58, 61, 62, 68, 72, 75, 76, 77, 78, 79, 86, 87, 89, 97, 98, 99, 101, 102, 104, 106, 107, 109, 111, 114, 116, 117, 119, 121, 123, 126, 128, 130, 131, 133, 134, 137, 140, 141, 142, 146, 147, 158, 159, 160, 166, 167, 170, 173, 174, 177,181, 182, 183, 184, 185, 188, 192, 194, 198, 203, 204, 205, 206, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 222, 224, 227, 228, 230, 232, 237, 238, 240, 242, 243, 244, 245, 246, 247, 248, 249, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 265, 268, 269, 270, 271, 272, 274 oraz 275, przy czym jeśli odmiana obejmuje podstawienie jednocześnie w pozycjach 103 i 76, to występuje również podstawienie w przynajmniej jednej z pozycji innych niż odpowiadające następującym pozycjom 27, 99, 101, 104, 107, 109, 123, 128, 166, 204,
PL 191 529 B1
206, 210, 216, 217, 218, 222, 260, 265 lub 274, korzystnie szczep Bacillus BG2036, Bacillus subtilis 1168 oraz B. licheniformis oraz B. lentus.
11. Kompozycja do czyszczenia, znamienna tym, że jako środek aktywny zawiera odmianę proteazy, która zawiera podstawienie aminowasowe w pozycji reszty odpowiadającej pozycji 103 oraz przynajmniej jednej z pozycji 236 i 245 w subtylizynie z Bacillus amyloliquefaciens o sekwencji nr 1 oraz podstawienie przynajmniej jednego aminokwasu w pozycji reszty odpowiadającej następującym pozycjom w tej subtylizynie: 1, 3, 4, 8, 10, 12, 13, 16, 17, 18, 19, 20, 21, 22, 24, 27, 33, 37, 38, 42, 43, 48, 55, 57, 58, 61, 62, 68, 72, 75, 76, 77, 78, 79, 86, 87, 89, 97, 98, 99, 101, 102, 104, 106, 107, 109, 111, 114, 116, 117, 119, 121, 123, 126, 128, 130, 131, 133, 134, 137, 140, 141, 142, 146, 147, 158, 159, 160, 166, 167, 170, 173, 174, 177,181, 182, 183, 184, 185, 188, 192, 194, 198, 203, 204, 205, 206, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 222, 224, 227, 228, 230, 232, 237, 238, 240, 242, 243, 244, 245, 246, 247, 248, 249, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 265, 268, 269, 270, 271, 272, 274 oraz 275, przy czym jeśli odmiana obejmuje podstawienie jednocześnie w pozycjach 103 i 76, to występuje również podstawienie w przynajmniej jednej z pozycji innych niż odpowiadające następującym pozycjom 27, 99, 101, 104, 107, 109, 123, 128, 166, 204, 206, 210, 216, 217, 218, 222, 260, 265 lub 274, korzystnie w ilości od 0,01% do 5% wagowych w proszkowych lub ciekłych środkach czyszczących.
12. Karma dla zwierząt, znamienna tym, że zawiera odmianę proteazy, która zawiera podstawienie aminowasowe w pozycji reszty odpowiadającej pozycji 103 oraz przynajmniej jednej z pozycji 236 i 245 w subtylizynie z Bacillus amyloliquefaciens o sekwencji nr 1 oraz podstawienie przynajmniej jednego aminokwasu w pozycji reszty odpowiadającej następującym pozycjom w tej subtylizynie: 1, 3, 4, 8, 10, 12, 13, 16, 17, 18, 19, 20, 21, 22, 24, 27, 33, 37, 38, 42, 43, 48, 55, 57, 58, 61, 62, 68, 72, 75, 76, 77, 78, 79, 86, 87, 89, 97, 98, 99, 101, 102, 104, 106, 107, 109, 111, 114, 116, 117, 119, 121, 123, 126, 128, 130, 131, 133, 134, 137, 140, 141, 142, 146, 147, 158, 159, 160, 166, 167, 170, 173, 174, 177,181, 182, 183, 184, 185, 188, 192, 194, 198, 203, 204, 205, 206, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 222, 224, 227, 228, 230, 232, 237, 238, 240, 242, 243, 244, 245, 246, 247, 248, 249, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 265, 268, 269, 270, 271, 272, 274 oraz 275, przy czym jeśli odmiana obejmuje podstawienie jednocześnie w pozycjach 103 i 76, to występuje również podstawienie w przynajmniej jednej z pozycji innych niż odpowiadające następującym pozycjom 27, 99, 101, 104, 107, 109, 123, 128, 166, 204, 206, 210, 216, 217, 218, 222, 260, 265 lub 274, korzystnie w ilości od 0,01% do 5% wagowych.
13. Kompozycja do obróbki tekstyliów, znamienna tym, że zawiera odmianę proteazy, która zawiera podstawienie aminowasowe w pozycji reszty odpowiadającej pozycji 103 oraz przynajmniej jednej z pozycji 236 i 245 w subtylizynie z Bacillus amyloliquefaciens o sekwencji nr 1 oraz podstawienie przynajmniej jednego aminokwasu w pozycji reszty odpowiadającej następującym pozycjom w tej subtylizynie: 1, 3, 4, 8, 10, 12, 13, 16, 17, 18, 19, 20, 21, 22, 24, 27, 33, 37, 38, 42, 43, 48, 55, 57, 58, 61, 62, 68, 72, 75, 76, 77, 78, 79, 86, 87, 89, 97, 98, 99, 101, 102, 104, 106, 107, 109, 111, 114, 116, 117, 119, 121, 123, 126, 128, 130, 131, 133, 134, 137, 140, 141, 142, 146, 147, 158, 159, 160, 166, 167, 170, 173, 174, 177,181, 182, 183, 184, 185, 188, 192, 194, 198, 203, 204, 205, 206, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 222, 224, 227, 228, 230, 232, 237, 238, 240, 242, 243, 244, 245, 246, 247, 248, 249, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 265, 268, 269, 270, 271, 272, 274 oraz 275, przy czym jeśli odmiana obejmuje podstawienie jednocześnie wpozycjach 103 i 76, to występuje również podstawienie w przynajmniej jednej z pozycji innych niż odpowiadające następującym pozycjom 27, 99, 101, 104, 107, 109, 123, 128, 166, 204, 206, 210, 216, 217, 218, 222, 260, 265 lub 274, korzystnie w ilości od 0,01% do 5% wagowych.
14. Odmiana proteazy według zastrz. 1, znamienna tym, że zawiera następujący zestaw podstawień wybrany z grupy obejmującej pozycje reszt odpowiadających pozycjom subtylizyny z Bacillus amyloliquefaciens:
PL 191 529 B1
76,103,104,222; 76,98,103,104; 76,78,103,104; 76,103,104,107; 4,76,103,104;
76,103,104,246; 76,77,103,104; 76,103,104,183,218; 16,76,103,104,248;
1,76,103,104; 76,103,104,261; 76,103,104,160; 76,103,104,216; 17,76,103,104;
37,76,103,104; 76,77,103,104,174; 38,76,103,104; 38,76,103,104,237;
8,76,103,104; 76,103,104,183; 19,76,103,104; 13,76,103,104; 19,76,103,104;
76,103,104,184; 76,103,104,252; 76,103,104,259; 76,103,104,251; 76,86,103,104;
72,76,103,104,185; 76,103,104,237,274; 76,103,104,160; 76,103,104,228;
55,76,103,104,240; 76,103,104,254; 76,103,104,204; 76,103,104,204;
43,76,103,104; 76,103,104,159; 10,76,103,104,177; 58,76,103,104;
76,103,104,270; 76,103,104,185; 27,76,103,104; 76,103,104,262; 76,78,103,104;
24,76,103,104; 76,103,104,166,236,251; 17,76,103,104,237; 76,103,104,130;
76,103,104,109; 76,99,103,104,204; 76,103,104,181; 12,76,103,104;
76,103,104,212,271; 76.103,104,252,261; 76,103,104,242; 76,103,104,271;
12,76,103,104,242:43,76,103,104,116,183: 76,103,104,258:76,103,104,271
PL 191 529 B1
61,76,103,104; 38,76,103,104,182,263; 76,103,104,182,272; 76,103,104,109,246;
76,87,103,104,206,249,265; 76,103,104,137,238,271; 103,104,228,;
76,103,104,182,198; 21,76,103,104,182; 76,103,104,119,137; 76,103,104,137,248; 13,76,103,104,206; 76,103,104,206; 76,103,104,212,258; 58,76,103,104,271;
76,103,104,206,261; 4,76,103,104,206; 76,77,103,104,206; 76,103,104,158;
76,103,104,206; 4,76,103,104,159,217,251; 4,76,103,104,159,217,252;
76,77,103,104,133,185,251; 76,103,104,159,206,244; 4,76,103,104,188;
4,76,103,104,158; 76,77,103,104,185; 76,103,104,206,251; 48,76,103,104,111,159; 68,76,103,104,159,236; 42,76,103,104,159; 12,62,76,103,104,159;
42,76,103,104,159; 76,103,104,146,159; 76,103,104,159,238; 76,103,104,159,224; 76,103,104,212,268,271; 76,89,103,104; 76,87,103,104,212,271;
76,103,104,212,245,271; 76,103,104,134,141,212,271;
76,103,104,212,236,243,271; 76,103,104,109,245; 76,103,104,109,210;
20,62,76,103,104; 68,76,103,104,236; 68,76,103,104,159,236,271;
68,76,103,104,159,236,245; 68,76,103,104,159,217,236,271; 17,68,76,103,104;
68,76,103,104; 68,76,103,104,159,236; 68,75,76,103,104,159,236;
68,76,76,103,114,121,159,236,245; 12,68,76,103,104,159,236;
68,76,103,104,159,209,236,253; 68,76,103,104,117,159,184,236;
68,76,103,104,159,236,243; 68,76,103,104,159,236,245; 68,76,103,104,142,159;
68,76,103,104,123,159,236,249; 68,76,103,104,159,236,249; 76,103,104,222,245;
12,76,103,104,222,249; 76,103,104,173,222; 76,103,104,222,263;
21,76,103,104,222,237,263; 76,103,104,109,222; 76,103,104,109,222,271;
61,76,103,104,222; 76,103,104,137,222; 76,103,104,109,222,248;
76,103,104,222,249:68,76,103,104,159,236,245,261;
68,76,103,104,141,159,236,245,255; 68,76,103,104,159,236,245,247;
PL 191 529 B1
68,76,103,104,159,174,204,236,245; 68,76,103,104,159,204,236,245;
68,76,103,104,133,159,218,236,245; 68,76,103,104,159,232,236,245; 68,76,103,104,159,194,203,236,245; 12,76,103,104,222,245; 76,103,104,232,245; 24,68,76,103,104,159,232,236,245; 68,103,104,159,232,236,245,252; 68,76,103,104,159,213,232,236,245,260; 12,76,103,104,222,244,245; 12,76,103,222,210,245; 12,76,103,104,130,222,245; 22,68,76,103,104;
68,76,103,104,184; 68,103,104,159,232,236,245,248,252; 68,103,104,159,232,236,245; 68,103,104,140,159,232,236,245,252; 43,68,103,104,159,232,236,245,252;43,68,103,104,159,232,236,245; 43,68,103,104,159,232,236,245,252; 68,87,103,104,159,232,236,245,252,275; 12,76,103,104,130,222,245,248,262; 12,76,103,104,130,215,222,245; 12,76,103,104,130,222,227,245,262; 12,76,103,104,130,222,245,261; 76,103,104,130,222,245; 12,76,103,104,130,218,222,245,262,269; 12,57,76,103,104,130,222,245,251; 12,76,103,104,130,170,185,222,243,245; 12,76,103,104,130,222,245,268; 12,76,103,104,130,222,210,245;
68,103,104,159,232,236,245,257; 68,103,104,116,159,232,236,245;
68,103,104,159,232,236,245,248; 10,68,103,104,159,232,236,245; 68,103,104,159,203,232,236,245; 68,103,104,159,232,236,237,245; 68,76,79,103,104,159,232,236,245; 68,103,104,159,183,232,236,245;
68,103,104,159,174,206,232,236,245; 68,103,104,159,188,232,236,245;
68,103,104,159,230,232,236,245; 68,98,103,104,159,232,236,245; 68,103,104,159,215,232,236,245; 68,103,104,159,232,236,245,248;
68,76,103,104,159,232,236,245; 68,76,103,104,159,210,232,236,245;
68,76,103,104,159,232,236,245,257; 76,103,104,232,236,245,257;
68,103,104,159,232,236,245,257,275; 76,103,104,257,275;
PL 191 529 B1
68,103,104,159,224,232,236,245,257; 76,103,104,159,232,236,245,257;
68,76,103,104,159,209,232,236,245; 68,76,103,104,159,211,232,236,245;
12,68,76,103,104,159,214,232,236,245; 68,76,103,104,159,215,232,236,245;
12,68,76,103,104,159,232,236,245:20,68,76,103,104,159,232,236,245,259; 68,87,76,103,104,159,232,236,245,260; 68,76,103,104,169,232,236,245,261; 76,103,104,232,236,242,245; 68,76,103,104,159,210,232,236,245;
12,48,68,76,103,104,159,232,236,245; 76,103,104,232,236,245;
76,103,104,159,192,232,236,245; 76,103,104,147,159,232,236,245,248,251;
12,68,76,103,104,159,232,236,245,272; 68,76,103,104,159,183,206,232,236,245;
.68,76,103,104,159,232,236,245,256; 68,76,103,104,159,206,232,236,245;
27,68,76,103,104,159,232,236,245; 68,76,103,104,116,159,170,185,232,236,245;
61,68,103,104,159,232,236,245,248,252; 43,68,103,104,159,232,236,245,248,252;
68,103,104,159,212,232,236,245,248,252;
68,103,104,99,159,184,232,236,245,248,252; 103,104,159,232,236,245,248,252;
68,103,104,159,209,232,236,245,248,252;
68,103,104,109,159,232,236,245,248,252;
20,68,103,104,159,232,236,245,248,252;
68,103,104,159,309,232,236,245,248,252;
68,103,104,159,232,236,245,248,252,261;
68,103,104,159,185,232,236,245,248,252;
68,103,104,159,210,232,236,245,248,252;
68,103,104,159,185,210,232,236,245,248,252;
68,103,104,159,212,232,236,245,248,252;
68,103,104,159,213,232,236,245,248,252; 68,103,104,213,232,236,245,248,252;
68,103,104,159,215,232,236,245,248,252;
PL 191 529 B1
68,103,104,159,216,232,236,245,248,252;
20,68,103,104,159,232,236,245,248,252;
68,103,104,159,173,232,236,245,248,252;
68,103,104,159,232,236,245,248,251,252;
68,103,104,159,206,232,236,245,248,252; 68,103,104,159,232,236,245,248,252; 55,68,103,104,159,232,236,245,248,252;
68,103,104,159,232,236,245,248,252,255;
68,103,104,159,232,236,245,248,252,256;
68,103,104,159,232,236,245,248,252,260;
68,103,104,159,232,236,245,248,252,257;
68,103,104,159,232,236,245,248,252,258;
8,68,103,104,159,232,236,245,248,252,269;
68,103,104,116,159,232,236,245,248,252,260;
68,103,104,159,232,236,245,248,252,261;
68,103,104,159,232,236,245,248,252,261;
68,76,103,104,159,232,236,245,248,252; 68,103,104,232,236,245,248,252;
103,104,159,232,236,245,248,252; 68,103,104,159,232,236,245,248,252; 18,68,103,104,159,232,236,245,248,252; 68,103,104,159,232,236,245,248,252; 68,76,101,103,104,159,213,218,232,236,245,260;
68,103,104,159,228,232,236,245,248,252;
33,68,76,103,104,159,232,236,245,248,252;
68,76,89,103,104,159,210,213,232,236,245,260;
61,68,76,103,104,159,232,236,245,248,252; 103,104,159,205,210,232,236,245;
61,68,103,104,130,159,232,236,245,248,252;
61,68,103,104,133,137,159,232,236,245,248,252;
PL 191 529 B1
61,103,104,133,159,232,236,245,248,252; 68,103,104,159,232,236,245,248,252; 68,103,104,159,218,232,236,245,248,252;
61,68,103,104,159,160,232,236,245,248,252;
3,61,68,76,103,104,232,236,245,248,252;
61,68,103,104,159,167,232,236,245,248,252;
97,103,104,159,232,236,245,248,252; 98,103,104,159,232,236,245,248,252; 99,103,104,159,232,236,245,248,252; 101,103,104,159,232,236,245,248,252; 102,103,104,159,232,236,245,248,252; 103,104,106,159,232,236,245,248,252; 103,104,109,159,232,236,245,248,252; 103,104,159,232,236,245,248,252,261; 62,103,104,159,232,236,245,248,252; 103,104,159,184,232,236,245,248,252; 103,104,159,166,232,236,245,248,252; 103,104,159,217,232,236,245,248,252; 20,62,103,104,159,213,232,236,245,248,252;
62,103,104,159,213,232,236,245,248,252;
103,104,159,206,217,232,236,245,248,252;
62,103,104,159,206,232,236,245,248,252; 103,104,130,159,232,236,245,248,252; 103,104,131,159,232,236,245,248,252; 27,103,104,159,2-32,236,245,248,252; 38,103,104,159,232,236,245,248,252; 38,76,103,104,159,213,232,236,245,260; 68,76,103,104,159,213,232,236,245,260,271;
68,76,103,104,159,209,213,232,236,245,260;
68,76,103,104,159,210,213,232,236,245,260;
68,76,103,104,159,205,213,232,236,245,260;
68,76,103,104,159,210,232,236,245,260; 68,103,104,159,213,232,236,245,260;
76,103,104,159,213,232,236,245,260; 68,103,104,159,209,232,236,245; 68,103,104,159,210,232,236,245; 68,103,104,159,230,232,236,245; 68,103,104,159,126,232,236,245; 68,103,104,159,205,232,236,245;
PL 191 529 B1
68,103,104,159,210,232,236,245; 103,104,159,230,236,245;
68,103,104,159,232,236,245,260; 103,104,159,232,236,245;
68,103,104,159,174,232,236,245,257; 68,103,104,159,194,232,236,245,257; 68,103,104,159,209,232,236,245,257; 103,104,159,232,236,245,257;
68,76,103,104,159,213,232,236,245,260,261;
68,103,104,159,232,236,245,257,261; 103,104,159,213,232,236,245,260;
103,104,159,210,232,236,245,248,252; 103,104,159,209,232,236,245,257;
68,76,103,104,159,210,213,232,236,245,260;
12,103,104,159,209,213,232,236,245,260; 103,104,209,232,236,245,257;
103,104,159,205,210,213,232,236,245,260; 103,104,159,205,209,232,236,245,260; 68,103,104,159,205,209,210,232,236,245;
103,104,159,205,209,210,232,236,245,257; 103,104,159,205,209,232,236,245,257; 68,103,104,159,205,209,210,232,236,245,260;
103,104,159,205,209,210,232,236,245; 103,104,159,209,210,232,236,245; 103,104,159,205,210,232,236,245; 68,103,104,128,159,232,236,245; 48,103,104,159,230,236,245; 48,68,103,104,169,209,232,236,245; 48,68,103,104,159,232,236,245,248,252; 48,68,103,104,159,232,236,245,257,261; 102,103,104,159,212,232,236,245,248,252;
12,102,103,104,159,212,232,236,245,248,252;
101,102,103,104,159,212,232,236,245,248,252;
98,102,103,104,159,212,232,236,245,248,252;
102,103,104,159,213,232,236,245,248,252; 103,104,131,159,232,236,245,248,252; 103,104,159,184,232,236,245,248,252; 103,104,159,232,236,244,245,248,252; 62,103,104,159,213,232,236,245,248,252,256;
12,62,103,104,159,213,232,236,245,248,252;
PL 191 529 B1
101,103,104,159,185,232,236,245,248,252;
101,103,104,159,206,232,236,245,248,252;
101,103,104,159,213,232,236,245,248,252;
98,102,103,104,159,232,236,245,248,252;
101,102,103,104,159,232,236,245,248,252;
98,102,103,104,159,212,232,236,245,248,252;
98,102,103,104,159,212,232,236,248,252;
62,103,104,109,159,213,232,236,245,248,252;
62,103,104,159,212,213,232,236,245,248,252;
62,101,103,104,159,212,213,232,236,245,248,252; 103,104,159,232,245,248,252; 103,104,159,230,245; 62,103,104,130,159,213,232,236,245,248,252;
101,103,104,130,159,232,236,245,248,252;
101,103,104,128,159,232,236,245,248,252;
62,101,103,104,159,213,232,236,245,248,252;
62,103,104,128,159,213,232,236,245,248,252;
62,103,104,128,159,213,232,236,245,248,252;
101,103,104,159,232,236,245,248,252,260;
101,103,104,131,159,232,236,245,248,252;
98,101,103,104,159,232,236,245,248,252;
99,101,103,104,159,232,236,245,248,252;
101,103,104,159,212,232,236,245,248,252;
101,103,104,159,209,232,236,245,248,252;
101,103,104,159,210,232,236,245,248,252;
101,103,104,159,205,232,236,245,248,252; 101,103,104,159,230,236,245;
101,103,104,159,194,232,236,245,248,252;
76,101,103,104,159,194,232,236,245,248,252;
101,103,104,159,230,232,236,245,248,252;
62,103,104,159,185,206,213,232,236,245,248,252,271.
PL 191 529 B1
15. Odmiana proteazy według zastrz. 14, znamienna tym, że zawiera następujący zestaw podstawień wybrany z grupy obejmującej pozycje reszt odpowiadających pozycjom subtylizyny z Bacillus amyloliquefaciens:
N76D, S103A, V104l, M222S; N76D, A98E, S103A, V104l; N76D, S78T, S103A,
Y104I; N76D, S103A, V104l, I107Y; V4E, N76D, S103A, V104I; N76D, S103A,
V104I, I246Y; N76D, N77D, S103A, V104I; N76D, S103A, V104l, N183D, N218I;
A16T, N76D, S103A, V104l, N248D; A1E, N76D, S103A, V104I; N76D, S103A,
V104l, N261D; N76D, S103A, V104l, S160T; N76D, S103A, Y104I, S216C; H17Q,
N76D, S103A, V104l; S37T, N76D, S103A, V104l; N76D, N77D, S103A, V104l,
A174V; T38S, N76D, S103A, V104I; T38S, N76D, S103A, V104l, K237Q; I8V,
N76D, S103A, V104l; N76D, S103A, V 1041, N183D; R19L, N76D, S103A, V104I;
A13V, N76D, S103A, V104I; R19C, N76D, S103A, V104l; N76D, S103A, V104l,
N184D; N76D, S103A, V104l, N252D; N76D, S103A, V104l, S259C; N76D,
S103A, V104l, K251T; N76D, P86S, S103A, V104l; I72V, N76D, S103A, V104l,
N185D; N76D, S103A, V104l, K237E, T274A; N76D, S103A, V104I, S160L;
N76D, S103A, V104l, A228Y; P55S, N76D, S103A, V104l, S240T; N76D, S103A,
V104l, A254T; N76D, S103A, I104N, N204T; N76D, S103A, V104l, N204D; N43S,
N76D, S103A, V104I; N76D, S103A, V104l, G159D; R10H, N76D, S103A, V104l,
Y177A; T58S, N76D, S103A, V104l; N76D, S103A, V104l, A270Y; N76D, S103A,
V104l, N185D; K27N, N76D, S103A, V104l; N76D, S103A, V104l, L262M; N76D,
S78P, S103A, V104l; S24P, N76D, S103A, V104l; N76D, S103A, V104l, S166G,
Q236R, K251R; H17L, N76D, S103A, V104l, K237E; N76D, S103A, V104l, S130L;
PL 191 529 B1
N76D, S103A, V104I, Q109R; N76D, S99R, S103A, V104I, N204T; N76D, S103A, V104l, D181N; Q12R, N76D, S103A, V104l; N76D, S103A, V104I, S212P, E271V; N76D, S103A, V104l, N252K, N261Y; N76D, S103A, V104I, S242T; N76D, S103A, V104l, E271Q; Q12R, N76D, S103A, V104l, S242T; N43S, N76D, S103A, V104l, N116K, N1831; N76D, S103A, V104l, G258R; N76D, S103A, V104l, E271G;
G61R, N76D, S103A, V104l; T38S, N76D, S103A, V104l, Q182R, Y263H; N76D, S103A, V104I, Q182R, A272S; N76D, S103A, V104I, Q109R, I246Y; N76D, S87G, S103A, V104l, Q206R, H249Q, S265G; N76D, S103A, V104I, Q137R, N238Y, E271V; S103A, V104l, A228T, ; N76D, S103A, V104l, Q182R, 1198 V; L21M, N76D, S103A, V104l, Q182R; N76D, S103A, V104l, M119I, Q137R; N76D, S103A, V104l, Q137R, N248S; A13T, N76D, S103A, V104I, Q206R; N76D, S103A, V104l, Q206R; N76D, S103A, Y104I, 8212P, G258R; T58S, N76D, S103A; V104l,
E271G; N76D, S103A; V104l, Q206E, N261D; V4E, N76D, S103A; V104l,
Q206E; N76D, N77D, S103A; V104l, Q206E; N76D, S103A; V104l, A158E;
N76D, S103A; V104l, Q206E; V4E, N76D, S103A; V104l, G159D, L217E, K251Q; V4E, N76D, S103A; V104l, G159D, L217E, N252D; N76D, N77D, S103A; V104l,
A1SST, N185D, K251T; N76D, S103A; V104l, G159D, Q206E, Y244A; V4E,
N76D, S103A; V104l,8188E; V4E, N76D, S103A; V104l,A158E; N76D, N77D, S103A; V104l, N185D; N76D, S103A; V104l, Q206E, K251T; A48T, N76D,
S103A; V104l, L111M, G159D; V68A, N76D, S103A; V104l, G159D, Q2S6H;
L42V, N76D, S103A; V104l, G159D; Q12H, N62H, N76D, S103A; V104l, G159D;
L421, N76D, S103A, V104I, G159D; N76D, S103A, Y104I, G146S, G159D; N76D,
S103A, V104l, G159D, N238S; N76D, S103A, Y104I, G159D, T224A; N76D, S103A, V104I, S212P, Y268F, E271Y; N76D, E89A, S103A, V104l; N76D, S87R,
S103A, V104l, S212P, E271Y; N76D, S103A, V104I, S212P, Q245L, E271V;
PL 191 529 B1
N76D, S103A, V104l, T1A4S, S141N, S212P, E271V; N76D, S103A, V104l, S212P, Q236L, N243S, E271V; N76D, S103A, V104l, Q109R, Q245R; N76D, S103A,
V104I, Q109R, P210L; G20V, N62S, N76D, S103A, V104l; V68A, N76D, S103A,
V104I, Q236H; V68A, N76D, S103A, V104l, G159D, Q236H, E271V; V68A, N76D, S103A, V104I, G159D, Q236H, Q245R; V68A, N76D, S103A, V104I, G159D,
L217I, Q236H, E271V; H17Q, V68A, N76D, S103A, V104l; V68A, N76D, S103A,
V104I; V68A, N76D, S103A, V104I, G159D, Q236R; V68A, L75R, N76D, S103A, V104l, G159D, Q236H; V68A, N76D, N76D, S103A, Α114Y, ¥1211, G159D, Q236H,Q245R; Q12R, V68A, N76D, S103A, V104l, G159D, Q236H; V68A, N76D, S103A, V104I, G159D, Y209S, Q236H, T253K; V68A, N76D, S103A, V104I,
N117K, G159D, N184S, Q236H; V68A, N76D, S103A, V104l, G159D, Ω236Η, N243I; V68A, N76D, S103A, V104l, G159D, Q236H, Q245L; V68A, N76D, S103A,
V104l, A142V, G159D; V68A, N76D, S103A, V104I, N123S, G159D, Q236H,
H249Y; V68A, N76D, S103A, V104I, G159D, Q236H, H249Q; N76D, S103A;
V104l, M222S, Q245R; N76D, S103A, V104l, Q12R, M222S, H249R; N76D,
S103A, V104l, N173R, M222S; N76D, S103A, V104l, M222S, Y263F; L21M,
N76D, S103A, V104I, M222S, K237R, Y263F; N76D, S103A, V104l, Q109R,
M222S; N76D, S103A, Y104I, Q109R, M222S, E271D; G61R, N76D, S103A,
V104I, M222S; N76D, S103A, V104l, Q137R, M222S; N76D, S103A, V104l,
Q109R, M222S, N248S; N76D, S103A, Y104I, M222S, H249R; V68A, N76D,
S103A, V104l, G159D, Q236H, Q245R, N261D; V68A, N76D, S103A, V104l,
S141N, G159D, Q236H, Q245R, T255S; V68A, N76D, S103A, V104l, G159D,
Q236H, Q245R, R247H; V68A, N76D, S103A, V104l, G159D, A174V, N204D,
Q236H, Q245R; V68A, N76D, S103A, V104l, G159D, N204D, Q236H, Q245R;
V68A, N76D, S103A, V104I, A133V, G159D, N218D, Q236H, Q245R; V68A, N76D,
PL 191 529 B1
S103A, V104Ι, G159D, A232V, Q236H, Q245R; V68A, N76D, S103A, V104l, G159D, A194I, Y203A, Q236H, Q245R; Q12R, N76D, S103A, V104l, M222S, Q245R; N76D, S103A, V104l, A232Y, Q245R; S24T, V68A, N76D, S103A, V104l, G159D, A232V, Q236H, Q245R; V68A, S103A, V104l, G159D, A232V, Q236H, Q245R, N252K; V68A, N76D, S103A, V104l, G159D, T213R, A232V, Q236H, Q245R, T260A; Q12R, N76D, S103A, I104T, M222S, Y244I, Q245R; Q12R, N76D, S103A, M222S, P210T, Q245R; Q12R, N76D, S103A, I104T, S130T, M222S, Q245R; T22K, V68A, N76D, S103A, V104l; V68A, N76D, S103A, N184D; V68A, S103A, V104l, G159D, A232V, Q236H, Q245R, N248D, N252K; V68A, S103A, Y104I, G159D, A232Y, Q236H, Q245R; V68A, S103A, V104l, N140D, G159D, A232V, Q236H, Q245R, N252K; N43S, V68A, S103A, V104l, G159D, A232V, Q236H, Q245R, N252K; N43K, V68A, S103A, V104l, G159D, A232V, Q236H, Q245R; N43D, V68A, S103A, V104l, G159D, A232V, Q236H, Q245R, N252K; V68A, S87G, S103A, V104l, G159D, A232V, Q236H, Q245R, N252K, R275S;
Q12R, N76D, S103A, I104T, S130T, M222S, Q245R, N248S, L262M; Q12R, N76D, S103A, I104T, S130T, A215Y, M222S, Q245R; Q12R, N76D, S103A, I104T,
S130T, M222S, V227A, Q245R, L262S; Q12R, N76D, S103A, I104T, S130T, A215T, M222S, Q245R; Q12R, N76D, S103A, I104T, S130T, M222S, Q245R, N261D; N76D, S103A, I104T, S130T, M222S, Q245R; Q12R, N76D, S103A,
I104T, S130T, N218D, M222S, Q245R, L262S, N269D; Q12R, S57P, N76D,
S103A, I104T, S130T, M222S, Q245R, K251Q; Q12R, N76D, S103A, I104T,
S130T, R170S, N185D, M222S, N243D, Q245R; Q12R, N76D, S103A, I104T, S130T, M222S, Q245R, Y268A; Q12R, N76D, S103A, I104T, S130T, M222S,
P210S, Q245R; V68A, S103A, V104I, G159D, A232Y, Q236H, Q245R, L257V; V68A, S103A, V104l, N116D, G159D, A232Y, Q236H, Q245R; V68A, S103A,
PL 191 529 B1
V1041, G159D, Α232Υ, Q236H, Q245R, N248D; R10C, V68A, S103A, V104l, G159D, A232Y, Q236H, Q245R; V68A, S103A, V104l, G159D, Y203E, A232Y, Ω236Η, Q245R; V68A, S103A, V104l, G159D, A232Y, Ω236Η, K237E, Q245R;
V68A, N76D, 179N, S103A, V104l, G159D, A232Y, Ω236Η, Q245R; V68A, S103A,
V104I, G159D, N183D, A232Y, Q236H, Q245R; V68A, S103A, V104I, G159D, A174Y, Q206L, A232Y, Ω236Η, Q245R; V68A, S103A, V104I, G159D, S188C, A232Y, Ω236Η, Q245R; V68A, S103A, V104l, G159D, A230T, A232Y, Q236H, Q245R; V68A, A98T, S103A, V104l, G159D, A232Y, Q236H, Q245R; V68A, S103A, V104I, G159D, A215T, A232Y, Q236H, Q245R; V68A, S103A, V104l, G159D, A232Y, Q236H, Q245R, N248S; V68A, N76D, S103A, V104l, G159D, A232Y, Q236H, Q245R; V68A, N76D, S103A, V104I, G159D, P210R, A232Y, Q236H, Q245R; V68A, N76D, S103A, V104l, G159D, A232Y, Q236H, Q245R, L257V; N76D, S103A, V104I, A232Y, Q236H, Q245R, L257V; V68A; S103A, V104l, G159D, A232Y, Q236H, Q245R, L257Y, R275H; N76D, S103A, V104l, L257Y, R275H, ; V68A, S103A, V104l, G159D, T224A, A232Y, Q236H, Q245R, L257Y; N76D, S103A, V104l, G159D, A232Y, Q236H, Q245R, L257Y; V68A,
N76D, S103A, V104l, G159D, Y209W, A232V, Q236H, Q245R; V68A, N76D,
S103A, V104I, G159D, G211R, A232V, Q236H, Q245R; V68A, N76D, S103A,
V104l, G159D, G211Y, A232V, Q236H, Q245R; Q12R, V68A, N76D, S103A,
V104l, G159D, Y214L, A232V, Q236H, Q245R; V68A, N76D, S103A, V104l,
G159D, A215R, A232V, Q236H, Q245R; Q12R, V68A, N76D, S103A, V104l,
G159D, A232V, Q236H, Q245R; G20R, V68A, N76D, S103A, V104l, G159D,
A232V, Q236H, Q245R, S259G; V68A, S87R, N76D, S103A, V104l, G159D,
A232V, Q236H, Q245R, T260Y; V68A, N76D, S103A, V104l, G159D, A232V,
Q236H, Q245R, N261G; V68A, N76D, S103A, V104I, G159D, A232Y, Q236H,
PL 191 529 B1
Q245R, N261W; N76D, S103A, V104I, A232Y, Q236H, S242P, Q245R; V68A, N76D, S103A, V104l, G159D, P210L, A232V, Q236H, Q245R; Q12R, A48Y, V68A, N76D, S103A, V104l, G159D, A232V, Q236H, Q245R; N76D, S103A, V104l, A232Y, Q236H, Q245R; N76D, S103A, V104l, G159D, Y192F, A232V, Q236H, Q245R; V68A, S103A, V104I, G159D, A232Y, Q236H, Q245R, L257Y, R275H; N76D, S103A, V104l, L257Y, R275H; V68A, S103A, V104l, G159D, T224A, A232Y,
Q236H, Q245R, L257V; N76D, S103A, V1041, G159D, A232Y, Q236H, Q245R, L257V; V68A, N76D, S103A, V104I, G159D, Y209W, A232V, Q236H, Q245R;
V68A, N76D, S103A, V104l, G159D, G211R, A232V, Q236H, Q245R; V68A, N76D,
S103A, V104I, G159D, G211Y, A232V, Q236H, Q245R; Q12R, V68A, N76D,
S103A, V104l, G159D, Y214L, A232V, Q236H, Q245R; V68A, N76D, S103A,
VfÓ4l, G159D, A215R, A232V, Q236H, Q245R; Q12R, V68A, N76D, S103A, V104l, G159D, A232V, Q236H, Q245R; G20R, V68A, N76D, S103A, V104l, G159D,
A232V, Q236H, Q245R, S259G; V68A, S87R, N76D, S103A, V104l, G159D,
A232V, Q236H, G245R, T260Y; V68A, N76D, S103A, V104l, G159D, A232V,
Q236H, Q245R, N261G; V68A, N76D, S103A, V104l, G159D, A232Y, Q236H,
Q245R, N261W; N76D, S103A, V104l, A232Y, Q236H, S242P, Q245R; V68A,
N76D, S103A, V104I, G159D, P210L, A232V, Q236H, Q245R; Q12R, A48V, V68A,
N76D, S103A, V104l, G159D, A232V, Q236H, Q245R; N76D, S103A, V104l,
A232Y, Q236H, Q245R; N76D, S103A, V104l, G159D, Y192F, A232V, Q236H,
Q245R; N76D, S103A, V104l, Y147I, G159D, A232V, Q236H, Q245R, N248S,
K251R; Q12R, V68A, N76D, S103A, V104l, G159D, A232V, Q236H, Q245R,
A272S; V68A, N76D, S103A, V104l, G159D, N183K, Q206L, A232V, Q236H,
Q245R; V68A, N76D, S103A, V104i, G159D, A232V, Q236H, Q245R, S256R;
V68A, N76D, S103A, V104I, G159D, Q206R, A232V, Q236H, Q245R; K27R, V68A,
PL 191 529 B1
N76D, S103A, V104l, G159D, A232V, Q236H, Q245R; V68A, N76D, S103A, V104l, N116T, G159D, R170S, N185S, A232V, Q236H, Q245R; G61E, V68A, S103A, V104l, G159D, A232V, Q236H, Q245R, N248D, N252K; N43D, V68A, S103A, V104l, G159D, A232V, Q236H, Q245R, N248D, N252K; V68A, S103A, V104l, G159D, S212P, A232V, Q236H, Q245R, N248D, N252K; V68A, S103A, V104l, S99N, G159D, N184D, A232V, Q236H, Q245R, N248D, N252K; S103A, V104l, G159D, A232Y, Q236H, Q245R, N248D, N252K; V68A, S103A, V1041, G159D, Y209W, A232V, Q236H, Q245R, N248D, N252K; V68A, S103A, V104l, 0109R, G159D, A232V, Q236H, Q245R, N248D, N252K; G20R, V68A, S103A, V104l, G159D, A232V, Q236H, Q245R, N248D, N252K; V68A, S103A, V104l, G159D, Y209F, A232V, Q236H, Q245R, N248D, N252K; V68A, S103A, V104l, G159D, A232V, Q236H, Q245R, N248D, N252K, N261 D; V68A, S103A, V104l, G159D, N185D, A232V, Q236H, Q245R, N248D, N252K; V68A, S103A, V104l, G159D, P210R, A232V, Q236H, Q245R, N248D, N252K; V68A, S103A, V104l, G159D, P210T, A232V, Q236H, Q245R, N248D, N252K; V68A, S103A, V104l, G159D, P210S, A232V, Q236H, Q245R, N248D, N252K; V68A, S103A, V104l, G159D, N185D, P210L, A232V, Q236H, Q245R, N248D, N252K; V68A, S103A, V1041, G159D, P210L, A232V, Q236H, Q245R, N248D, N252K; V68A, S103A, V104l, G159D, S212A, A232V, Q236H, Q245R, N248D, N252K; V68A, S103A, V104l, G159D, S212G, A232V, Q236H, Q245R, N248D, N252K; V68A, S103A, V104l, G159D, S212E, A232V, Q236H, Q245R, N248D, N252K; V68A, S103A, V104l, G159D, T213E, A232V, Q236H, Q245R, N248D, N252K; V68A, S103A, V104l, T213S, A232V, Q236H, Q245R, N248D, N252K; V68A, A103V, V104l, G159D, T213E, A232V, Q236H, Q245R, N248D, N252K; V68A, S103A, V104l, G159D, T213R, A232V, Q236H, Q245R, N248D, N252K; V68A, S103A, V104l, G159D,
PL 191 529 B1
T213G, A232V, Q236H, Q245R, N248D, N252K; V68A, S103A, V104I, G159D, A215Y, A232V, Q236H, Q245R, N248D, N252K; V68A, S103A, V104I, G159D,
A215R, A232V, Q236H, Q245R, N248D, N252K; V68A, S103A, V104I, G159D, S216T, A232V, Q236H, Q245R, N248D, N252K; V68A, S103A, V104l, G159D, S216Y, A232V, Q236H, Q245R, N248D, N252K; V68A, S103A, V104l, G159D, S216C, A232V, Q236H, Q245R, N248D, N252K; G20A, V68A, S103A, V104l, G159D, A232V, Q236H, Q245R, N248D, N252K; V68A, S103A, V104l, G159D, N173D, A232V, Q236H, Q245R, N248D, N252K; V68A, S103A, V104l, G159D, A232V, Q236H, Q245R, N248D, K251Y, N252K; V68A, S103A, V104l, G159D, Q206R, A232V, Q236H, Q245R, N248D, N252K; V68A, S103A, V104l, G159D, A232V, Q236H, Q245R, N248D, N252F; V68A, S103A, V1041, G159D, A232V, Q236H, Q245R, N248D, N252L; P55S, V68A, S103A, V104I, G159D, A232V, Q236H, Q245R, N248D, N252F; V68A, S103A, V104l, G159D, A232V, Q236H,
Q245R, N248D, N252K, T255Y; V68A, S103A, V104l, G159D, A232V, Q236H, Q245R, N248D, N252K, S256N; V68A, S103A, V104I, G159D, A232V, Q236H,
Q245R, N248D, N252K, S256E; V68A, S103A, V104l, G159D, A232V, Q236H,
Q245R, N248D, N252K, S256R; V68A, S103A, V104l, G159D, A232V, Q236H,
Q245R, N248D, N252K, T260R; V68A, S103A, V104l, G159D, A232V, Q236H,
Q245R, N248D, N252K, L257R; V68A, S103A, V104l, G159D, A232V, Q236H,
Q245R, N248D, N252K, G258D; 18V, V68A, S103A, V104l, G159D, A232V, Q236H, Q245R, N248D, N252K, N269D; V68A, S103A, V104l, N116S, G159D,
A232V, Q236H, Q245R, N248D, N252K, T260E; V68A, S103A, V104l, G159D,
A232V, Q236H, Q245R, N248D, N252K, N261R; V68A, S103A, V104l, G159D,
A232V, Q236H, Q245R, N248D, N252K, N261D; V68A, N76D, S103A, V104l,
G159D, A232V, Q236H, Q245R, N248D, N252K; V68A, S103A, V104l, A232V,
PL 191 529 B1
Q236H, Q245R, N248D, N252K; S103A, V104l, G159D, A232S, Q236H, Q245R, N248D, N252K; V68A, S103A, V104l, G159DD; A232Y, Q236R, Q245R, N248D, N252K; N18S, V68A, S103A, V104l, G159D, A232V, Q236H, Q245R, N248D, N252K; V68A, S103A, V104l, G159D, A232Y, Q236H, Q245V, N248D, N252K; V68A, N76D, S101T, S103A, V104l, G159D, T213R, N218S, A232V, Q236H, Q245R, T260A; V68A, S103A, V104l, G159D, A228Y, A232V, Q236H, Q245R, N248D, N252K; T33S, V68A, N76D, S103A, V104l, G159D, A232V, Q236H, Q245R, N248D, N252K; V68A, N76D, E89D, S103A, V104l, G159D, P210L, T213R, A232V, Q236H, Q245R, T260A; G61E, V68A, N76D, S103A, V104l, G159D, A232V, Q236H, Q245R, N248D, N252K; S103A, V104l, G159D, V205l, P210I, A232V, Q236H, Q245R; G61E, V68A, S103A, V104l, S130A, G159D, A232V, Q236H, Q245R, N248D, N252K; G61E, V68A, S103A, V104l, A133S, Q137R, G159D, A232V, Q236H, Q245R, N248D, N252K; G61E, S103A, V104l, A133Y, G159D, A232V, Q236H, Q245R, N248D, N252K; V68A, S103A, V104l, G159D, A232V, Q236H, Q245R, N248G, N252K; V68A, S103A, V104l, G159D, N218S, A232V, Q236H, Q245R, N248D, N252K; G61E, V68A, S103A, V104l, G159D, S 160 V, A232V, Q236H, Q245R, N248D, N252K; S3L, G61E, V68A, N76D, S103A, V104l, A232V, Q236H, Q245R, N248D, N252K; G61E, V68A, S103A, V104I, G159D, S167F, A232V, Q236H, Q245R, N248D, N252K; G97E, S103A, V104l, G159D, A232V, Q236H, Q245R, N248D, N252K; A98D, S103A, V104l, G159D, A232V, Q236H, Q245R, N248D, N252K; S99E, S103A, V104l, G159D, A232V, Q236K, Q245R, N248D, N252K; S101E, S103A, V104l, G159D, A232V, Q236H, Q245R, N248D, N252K; S101G, S103A, V104l, G159D, A232V, Q236H, Q245R, N248D, N252K; G102A, S103A, V104l, G159D, A232V, Q236H, Q245R, N248D, N252K; S103A, V104l, S106E, G159D, A232V, Q236H, Q245R,
PL 191 529 B1
N248D, Ν252Κ; S103A, V104l, Q109E, G159D, A232V, Q236H, Q245R, N248D, N252K; S103A, V104l, G159D, A232V, Q236H, Q245R, N248D, N252K, N261 R; S103A, V104l, Q109R, G159D, A232V, Q236H, Q245R, N248D, N252K; N62D, S103A, V104l, G159D, A232V, Q236H, Q245R, N248D, N252K; S103A, V104l, G159D, N184D, A232V, Q236H, Q245R, N248D, N252K; S103A, V104l, G159D, S166D, A232V, Q236H, Q245R, N248D, N252K; S103A, V104l, G159D, L217E, A232V, Q236H, Q245R, N248D, N252K; G20R, N62D, S103A, V104l, G159D, T213R, A232V, Q236H, Q245R, N248D, N252K; N62D, S103A, V104l, G159D, T213R, A232V, Q236H, Q245R, N248D, N252K; S103A, V104l, G159D, Q206R, L217E, A232V, Q236H, Q245R, N248D, N252K; N62D, S103A, V104l, G159D, Q206R, A232V, Q236H, Q245R, N248D, N252K; S103A, V104l, S130G, G159D, A232V, Q236H, Q245R, N248D, N252K; S103A, V104l, P131Y, G159D, A232V, Q236H, Q245R, N248D, N252K; K27N, S103A, V104l, G159D, A232V, Q236H, Q245R, N248D, N252K; T38G, S103A, V104l, G159D, A232V, Q236H, Q245R, N248D, N252K; T38A, N76D, S103A, V104l, G159D, T213R, A232V, Q236H, Q245R, T260A; V68A, N76D, S103A, V104l, G159D, T213R, A232V, Q236H, Q245R, T260A, E271G; V68A, N76D, S103A, V104l, G159D, Y209W, T213R, A232V, Q236H, Q245R, T260A; V68A, N76D, S103A, V104l, G159D, P210I, T213R, A232V, Q236H, Q245R, T260A; V68A, N76D, S103A, V104l, G159D, Y205I, T213R, A232V, Q236H, Q245R, T260A; V68A, N76D, S103A, V104l, G159D, P210I, A232V, Q236H, Q245R, T260A; V68A, S103A, V104l, G159D, T213R, A232V, Q236H, Q245R, T260A; N76D, S103A, V104l, G159D, T213R, A232V, Q236H, Q245R, T260A; V68A, S103A, V104l, G159D, Y209W, A232V, Q236H, Q245R; V68A, S103A, V104l, G159D, P210I, A232V, Q236H, Q245R; V68A, S103A, V104l, G159D, A230Y, A232V, Q236H, Q245R; V68A, S103A,
PL 191 529 B1
V104l, G159D, L126F, A232V, Q236H, Q245R; V68A, S103A, V104l, G159D, Y205I, A232V, Q236H, Q245R; V68A, S103A, V104I, G159D, P210L, A232V, Q236H, Q245R; S103A, V104l, G159D, A230Y, Q236H, Q245R; V68A, S103A, V104l, G159D, A232V, Q236H, Q245R, T260A; S103A, V104l, G159D, A232V, Q236H, Q245R; V68A, S103A, V104l, G159D, A174V, A232V, Q236H, Q245R, L257V; V68A, S103A, V1041, G159D, A194S, A232V, Q236H, Q245R, L257V; V68A, S103A, V1041, G159D, Y209W, A232V, Q236H, Q245R, L257V; S103A, V1041, G159D, A232V, Q236H, Q245R, L257V; V68A, N76D, S103A, V1041, G159D, T213R, A232V, Q236H, Q245R, T260A, N261W; V68A, S103A, V1041, G159D, A232V, Q236H, Q245R, L257V, N261W; S103A, V1041, G159D, T213R, A232V, Q236H, Q245R, T260A; S103A, V1041, G159D, P210I, A232V, Q236H, Q245R, N248D, N252K; S103A, Y104I, G159D, Y209W, A232V, Q236H, Q245R, L257V; V68A, N76D, S103A, V104l, G159D, P210L, T213R, A232V, Q236H, Q245R, T260A; Q12R, S103A, V1041, G159D, Y209W, T213R, A232V, Q236H, Q245R, T260A; S103A, V1041, Y209W, A232V, Q236H, Q245R, L257V, *; S103A, V1041, G159D, V205l, P210I, T213R, A232V, Q236H, Q245R, T260A; S103A, Y104I, G159D, V205l, Y209W, A232V, Q236H, Q245R, T260A; V68A, S103A, V1041, G159D, V205l, Y209W, P210I, A232V, Q236H, Q245R; S103A, V1041, G159D, V205l, Y209W, P210I, A232V, Q236H, Q245R, L257V; S103A, V1041, G159D, V205l, Y209W, A232V, Q236H, Q245R, L257V; V68A, S103A, V1041, G159D, V205l, Y209W, P210I, A232V, Q236H, Q245R, T260A; S103A, V1041, G159D, V205l, Y209W, P210I, A232V, Q236H, Q245R; S103A, V1041, G159D, Y209W, P210I, A232V, Q236H, Q245R; S103A, V104l, G159D, V205l, P210I, A232V, Q236H, Q245R; V68A, S103A, V104l, S128L, G159D, A232V, Q236H, Q245R; A48V, S103A, V104l, G159D, A230Y, Q236H, Q245R; A48V, V68A,
PL 191 529 B1
S103A, V104Ι, G159D, Y209W, A232V, Q236H, Q245R; A48V, V68A, S103A, V104l, G159D, A232V, Q236H, Q245R, N248D, N252K; A48V, V68A, S103A, V104l, G159D, A232V, Q236H, Q245R, L257Y, N261W; G102A, S103A, V1041, G159D, S212G, A232V, Q236H, Q245R, N248D, N252K; Q12R, G102A, S103A, V104l, G159D, S212G, A232V, Q236H, Q245R, N248D, N252K; S101G, G102A, S103A, V104l, G159D, S212G, A232V, Q236H, Q245R, N248D, N252K; A98L, G102A, S103A, V104I, G159D, S212G, A232V, Q236H, Q245R, N248D, N252K; G102A, S103A, V104l, G159D, T213R, A232V, Q236H, Q245R, N248D, N252K; S103A, Y104I, P131Y, G159D, A232V, Q236H, Q245R, N248D, N252K; S103A, Y104I, G159D, N184S, A232V, Q236H, Q245R, N248D, N252K; S103A, Y104I, G159D, N184G, A232V, Q236H, Q245R, N248D, N252K; S103A, V104l, G159D, A232Y, Q236H, V244T, Q245R, N248D, N252K; S103A, V104l, G159D, A232V, Q236H, Y244A, Q245R, N248D, N252K; N62D, S103A, V104l, G159D, T213R, A232V, Q236H, Q245R, N248D, N252K, S256R; Q12R, N62D, S103A, V104l, G159D, T213R, A232V, Q236H, Q245R, N248D, N252K; S101G, S103A, V104l, G159D, N185D, A232V, Q236H, Q245R, N248D, N252K; S101G, S103A, V104I, G159D, Q206E, A232V, Q236H, Q245R, N248D, N252K; S101G, S103A, V104I, G159D, T213Q, A232V, Q236H, Q245R, N248D, N252K; A98L, G102A, S103A, V104l, G159D, A232V, Q236H, Q245R, N248D, N252K; S101G, G102A, S103A, V104t, G159D, A232V, Q236H, Q245R, N248D, N252K; A98L, G102A, S103A, V104l, G159D, S212G, A232V, Q236H, Q245R, N248D, N252K; A98L, G102A, S103A, V104I, G159D, S212G, A232V, Q236H, N248D, N252K; N62D, S103A, V104l, Q109R, G159D, T213R, A232V, Q236H, Q245R, N248D, N252K; N62D, S103A, V104I, G159D, S212G, T213R, A232V, Q236H, Q245R, N248D, N252K; N62D, S101G, S103A, V104l, G159D, S212G, T213R, A232V, Q236H, Q245R,
PL 191 529 B1
N248D, Ν252Κ; S103A, V104l, G159D, A232V, Q245R, N248D, Ν252Κ; S103A,
V104I, G159D, A230V, Q245R; N62D, S103A, V104l, S130G, G159D, T213R,
A232V, Q236H, Q245R, N248D, N252K; S101G, S103A, V104l, S130G, G159D,
A232V, Q236H, Q245R, N248D, N252K; S101G, S103A, V104l, S128G, G159D,
A232V, Q236H, Q245R, N248D, N252K; S101G, S103A, V104l, S128L, G159D,
A232V, Q236H, Q245R, N248D, N252K; N62D, S101G, S103A, V104l, G159D,
T213R, A232V, Q236H, Q245R, N248D, N252K; N62D, S103A, V104I, S128G,
G159D, T213R, A232V, Q236H, Q245R, N248D, N252K; N62D, S103A, V104l,
S128L, G159D, T213R, A232V, Q236H, Q245R, N248D, N252K; S101G, S103A,
V104I, G159D, A232V, Q236H, Q245R, N248D, N252K, T260A; S101G, S103A,
V104I, P131Y, G159D, A232V, Q236H, Q245R, N248D, N252K; A98V, S101G,
S103A, V104I, G159D, A232V, Q236H, Q245R, N248D, N252K; S99G, S101G,
S103A, V104l, G159D, A232V, G236H, Q245R, N248D, N252K; S101G, S103A,
V104I, G159D, S212G, A232V, Q236H, Q245R, N248D, N252K; S101G, S103A,
V104I, G159D, Y209W, A232V, Q236H, Q245R, N248D, N252K; S101G, S103A,
V104I, G159D, P210I, A232V, Q236H, Q245R, N248D, N252K; S101G, S103A,
V104l, G159D, Y205I, A232V, Q236H, Q245R, N248D, N252K; S101G, S103A,
V104l, G159D, A230Y, 0236H, Q245R; S101G, S103A, V104l, G159, A194P,
A232V, Q236H, Q245R, N248D, N252K; N76D, S101G, S103A, V104l, G159D,
A194P, A232V, Q236H, Q245R, N248D, N252K; S101G, S103A, V104l, G159D,
A230Y, A232V, Q236H, Q245R, N248D, N252K; N62D, S103A, V104l, G159D,
N185D, 0206E, T213R, A232V, Q236H, Q245R, N248D, N252K, E271Q.
16. Odmiana proteazy według zastrz. 14, znamienna tym, że zawiera następujący zestaw podstawień wybrany z grupy obejmującej pozycje reszt odpowiadających pozycjom subtylizyny z Bacillus amyloliquefaciens przedstawionym: 76, 103, 104, 222, 245; 76, 103, 104, 222, 249; 68, 103, 104, 159, 232, 236, 245, 252; 68, 76, 103, 104, 159, 213, 232, 236, 245, 260; 22, 68, 76, 103, | 104; 68, 103, 104, 159, 232, 236, 245, 248, 252; 68, 103, 104, 159, 232, 236, 245; 68, 103, 104, 140, 159, 232, 236, 245, 252; 43, 68, 103, 104, 159, 232, 236, 245, 252; 43, 68, 103, 104, 159, 232, 236, 245; 12, 76, 103, 104, 130, 222, 245, 261; 76, 103, 104, 130, 222, 245; 68, 103, 104, 159, 232, 236, 245, 257; 68, 76, 103, 104, 159, 210, 232, 236, 245; 68, 103, 104, 159, 224, 232, 236, 245, 257; 76, 103, 10 104, 159, 232, 236, 245, 257; 68, 76, 103, 104, 159, 211, 232, 236, 245; 12, 68, 76, 103, 104, 159, 214, 232, 236, 245; 68, 76, 103, 104, 159, 215, 232, 236, 245; 12, 68, 76, 103, 104, 159, 232, 236, 245; 20, 68, 76, 103, 104, 159, 232, 236, 245, 259; 68, 76, 87, 103, 104, 159, 232, 236, 245, 260; 68, 76, 103, 104, 159, 232, 236, 245, 261; 12, 48, 68, 76, 103, 104, 159, 232, 236, 245; 76, 103, 104, 159, 15 192, 232, 236, 245; 76, 103, 104, 147, 159, 232, 236, 245, 248, 251; 12, 68, 76, 103, 104, 159, 232, 236, 245, 272; 68, 76, 103, 104, 159, 183, 206, 232, 236, 245; 68, 76, 103, 104, 159', 232, 236, 245, 256; 68, 76, 103, 104, 159, 206, 232, 236, 245; 27, 68, 76, 103, 104, 159, 232, 236, 245; 68, 103, 104, 159, 212, 232, 236, 245, 248, 252; 103, 104, 159, 232, 236, 245, 248, 252; 68, 103, 104, 159, 209, 20 232,
PL 191 529 B1
236, 245, 248, 252; 68, 103, 104, 109, 159, 232, 236, 245, 248, 252; 20, 68, 103, 104, 159, 232, 236, 245, 248, 252; 68, 103, 104, 159, 209, 232, 236, 245, 248, 252; 68, 103, 104, 159, 210, 232, 236, 245, 248, 252; 68, 103, 104, 159, 212, 232, 236, 245, 248, 252; 68, 103, 104, 159, 213, 232, 236, 245, 248, 252; 68, 103, 104, 213, 232, 236, 245, 248, 252; 68, 103, 104, 159, 215, 232, 236, 245, 248, 252; 68, 25 103, 104, 159, 216, 232, 236, 245, 248, 252; 20, 68, 103, 104, 159, 232, 236, 245, 248, 252; 68, 103, 104, 159, 232, 236, 245, 248, 252, 255; 68, 103, 104, 159, 232, 236, 245, 248, 252, 256; 68, 103, 104, 159, 232, 236, 245, 248, 252, 260; 68, 103, 104, 159, 228, 232, 236, 245, 248, 252; 68, 76, 89, 103, 104, 159, 210, 213, 232, 236, 245, 260; 68, 103, 104, 159, 218, 232, 236, 245, 248, 252.
Rysunki
PL 191 529 B1
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FIG..1B-2
PL 191 529 B1
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PORÓWNANIE SEKWENCJI SUBTILIZYN Z: B. amyloliquefaciens B. subtilis
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Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US95632397A | 1997-10-23 | 1997-10-23 | |
| US95656497A | 1997-10-23 | 1997-10-23 | |
| US95632497A | 1997-10-23 | 1997-10-23 | |
| PCT/US1998/022572 WO1999020770A2 (en) | 1997-10-23 | 1998-10-23 | Multiply-substituted protease variants |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| PL340600A1 PL340600A1 (en) | 2001-02-12 |
| PL191529B1 true PL191529B1 (pl) | 2006-06-30 |
Family
ID=27420734
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PL340600A PL191529B1 (pl) | 1997-10-23 | 1998-10-23 | Nowa odmiana proteazy, cząsteczka DNA kodująca nową odmianę proteazy, wektor zawierający tę cząsteczkę oraz karma dla zwierząt i kompozycje zawierające nową odmianę proteazy |
Country Status (26)
| Country | Link |
|---|---|
| US (5) | US6312936B1 (pl) |
| EP (10) | EP1025239B1 (pl) |
| JP (7) | JP2001520307A (pl) |
| KR (6) | KR100564039B1 (pl) |
| CN (9) | CN1195857C (pl) |
| AR (10) | AR016969A1 (pl) |
| AT (9) | ATE314478T1 (pl) |
| AU (7) | AU758371B2 (pl) |
| BR (6) | BR9813260A (pl) |
| CA (9) | CA2308206C (pl) |
| CZ (6) | CZ20001479A3 (pl) |
| DE (7) | DE69841226D1 (pl) |
| DK (9) | DK1025240T3 (pl) |
| EG (2) | EG22075A (pl) |
| ES (7) | ES2319470T5 (pl) |
| HK (1) | HK1039159B (pl) |
| HU (3) | HUP0104539A3 (pl) |
| ID (4) | ID25637A (pl) |
| MA (2) | MA25044A1 (pl) |
| NO (3) | NO326992B1 (pl) |
| NZ (3) | NZ520771A (pl) |
| PL (1) | PL191529B1 (pl) |
| PT (8) | PT1027418E (pl) |
| TR (8) | TR200001113T2 (pl) |
| TW (2) | TW585912B (pl) |
| WO (5) | WO1999020769A2 (pl) |
Families Citing this family (627)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MA23346A1 (fr) * | 1993-10-14 | 1995-04-01 | Genencor Int | Variantes de la subtilisine |
| ATE385254T1 (de) * | 1997-08-29 | 2008-02-15 | Novozymes As | Proteasevarianten und zusammensetzungen |
| AR016969A1 (es) * | 1997-10-23 | 2001-08-01 | Procter & Gamble | VARIANTE DE PROTEASA, ADN, VECTOR DE EXPRESIoN, MICROORGANISMO HUESPED, COMPOSICIoN DE LIMPIEZA, ALIMENTO PARA ANIMALES Y COMPOSICIoN PARA TRATAR UN TEXTIL |
| ES2368718T3 (es) | 1997-10-23 | 2011-11-21 | Danisco Us Inc. | Variantes de subtilisina con múltiples sustituciones. |
| ATE407941T1 (de) | 1998-07-02 | 2008-09-15 | Univ Toronto | Mit einer kohlenhydratgruppe chemisch modifizierte proteine |
| AR020951A1 (es) * | 1998-10-23 | 2002-06-05 | Procter & Gamble | Metodos para enmascarar variantes de proteasa para su utilizacion en composiciones detergentes |
| JP4932990B2 (ja) | 1998-11-10 | 2012-05-16 | ダニスコ・ユーエス・インコーポレイテッド | 化学修飾突然変異体セリンヒドロラーゼは改良された触媒活性およびキラル選択性を示す |
| EP1185302B1 (en) * | 1999-04-28 | 2013-07-24 | Genencor International, Inc. | Specifically targeted catalytic antagonists and uses thereof |
| US6627744B2 (en) | 1999-07-02 | 2003-09-30 | Genencor International, Inc. | Synthesis of glycodendrimer reagents |
| CZ2002171A3 (cs) * | 1999-07-22 | 2002-06-12 | The Procter & Gamble Company | Proteinázový konjugát, čistící prostředek a prostředek osobní péče |
| CA2376045A1 (en) * | 1999-07-22 | 2001-02-01 | The Procter & Gamble Company | Subtilisin protease variants having amino acid substitutions in defined epitope regions |
| EP1212397A1 (en) * | 1999-09-09 | 2002-06-12 | The Procter & Gamble Company | A detergent composition containing a protease |
| US6899961B2 (en) * | 1999-12-15 | 2005-05-31 | Samsung Sdi Co., Ltd. | Organic electroluminescence device |
| US7067049B1 (en) * | 2000-02-04 | 2006-06-27 | Exxonmobil Oil Corporation | Formulated lubricant oils containing high-performance base oils derived from highly paraffinic hydrocarbons |
| DE10007608A1 (de) * | 2000-02-18 | 2001-08-30 | Henkel Kgaa | Protease und Percarbonat enthaltende Wasch- und Reinigungsmittel |
| WO2001064852A1 (en) * | 2000-03-03 | 2001-09-07 | Novozymes A/S | Polypeptides having alkaline alpha-amylase activity and nucleic acids encoding same |
| US6777218B1 (en) * | 2000-03-14 | 2004-08-17 | Novozymes A/S | Subtilase enzymes having an improved wash performance on egg stains |
| JP2004508011A (ja) * | 2000-04-03 | 2004-03-18 | マキシジェン， インコーポレイテッド | スブチリシン変異体 |
| EP2258835A1 (en) * | 2000-04-28 | 2010-12-08 | Novozymes A/S | Lipolytic enzyme variant |
| CA2414158A1 (en) * | 2000-06-30 | 2002-01-10 | The Procter & Gamble Company | Detergent compositions comprising a maltogenic alpha-amylase enzyme |
| MXPA03000119A (es) * | 2000-06-30 | 2004-01-20 | Procter & Gamble | Composiciones detergentes que comprenden una enzima ciclodextrin glucanotransferasa. |
| CA2417547A1 (en) | 2000-07-28 | 2003-01-28 | Henkel Kommanditgesellschaft Auf Aktien | Novel amylolytic enzyme extracted from bacillus sp. a 7-7 (dsm 12368) and washing and cleaning agents containing this novel amylolytic enzyme |
| US20020155574A1 (en) | 2000-08-01 | 2002-10-24 | Novozymes A/S | Alpha-amylase mutants with altered properties |
| US6893855B2 (en) * | 2000-10-13 | 2005-05-17 | Novozymes A/S | Subtilase variants |
| US6803222B2 (en) * | 2000-11-22 | 2004-10-12 | Kao Corporation | Alkaline proteases |
| US7303907B2 (en) | 2001-01-08 | 2007-12-04 | Health Protection Agency | Degradation and detection of TSE infectivity |
| EP1241112A3 (en) | 2001-03-15 | 2003-02-26 | The Procter & Gamble Company | Flexible multiple compartment pouch |
| EP1373296B1 (en) * | 2001-03-23 | 2011-10-05 | Genencor International, Inc. | Proteins producing an altered immunogenic response and methods of making and using the same |
| EP2159279A3 (en) | 2001-05-15 | 2010-05-12 | Novozymes A/S | Alpha-amylase variant with altered properties |
| DK200101090A (da) | 2001-07-12 | 2001-08-16 | Novozymes As | Subtilase variants |
| DE10153792A1 (de) | 2001-10-31 | 2003-05-22 | Henkel Kgaa | Neue Alkalische Protease-Varianten und Wasch- und Reinigungsmittel enthaltend diese neuen Alkalischen Protease-Varianten |
| DE10162728A1 (de) | 2001-12-20 | 2003-07-10 | Henkel Kgaa | Neue Alkalische Protease aus Bacillus gibsonii (DSM 14393) und Wasch-und Reinigungsmittel enthaltend diese neue Alkalische Protease |
| DE10162727A1 (de) | 2001-12-20 | 2003-07-10 | Henkel Kgaa | Neue Alkalische Protease aus Bacillus gibsonii (DSM 14391) und Wasch-und Reinigungsmittel enthaltend diese neue Alkalische Protease |
| DE10163884A1 (de) | 2001-12-22 | 2003-07-10 | Henkel Kgaa | Neue Alkalische Protease aus Bacillus sp. (DSM 14392) und Wasch- und Reinigungsmittel enthaltend diese neue Alkalische Protease |
| US7332320B2 (en) * | 2001-12-31 | 2008-02-19 | Genencor International, Inc. | Protease producing an altered immunogenic response and methods of making and using the same |
| ATE450606T1 (de) * | 2002-01-16 | 2009-12-15 | Genencor Int | Mehrfachsubstituierte proteasevarianten |
| MXPA04006811A (es) * | 2002-01-16 | 2004-10-11 | Genencor Int | Variantes de proteasa substituidas con multiplos. |
| US7223386B2 (en) * | 2002-03-11 | 2007-05-29 | Dow Corning Corporation | Preparations for topical skin use and treatment |
| US20030180281A1 (en) * | 2002-03-11 | 2003-09-25 | Bott Richard R. | Preparations for topical skin use and treatment |
| DK1495128T3 (da) | 2002-03-29 | 2014-08-11 | Genencor Int | Forstærket proteinekspression i Bacillus |
| DK1545235T3 (da) | 2002-07-29 | 2014-01-27 | Zymtech Production As | Fremgangsmåde til fremstilling af peptider og aminosyrer fra proteinholdigt materiale af animalsk oprindelse |
| US7082689B2 (en) * | 2002-10-11 | 2006-08-01 | Black & Decker Inc. | Keyless shoe lock for reciprocating saw |
| US7888093B2 (en) * | 2002-11-06 | 2011-02-15 | Novozymes A/S | Subtilase variants |
| TWI319007B (en) * | 2002-11-06 | 2010-01-01 | Novozymes As | Subtilase variants |
| DE10260903A1 (de) * | 2002-12-20 | 2004-07-08 | Henkel Kgaa | Neue Perhydrolasen |
| US20050059567A1 (en) * | 2003-09-11 | 2005-03-17 | The Procter & Gamble Company | Methods of formulating enzyme cocktails, enzyme cocktails for the removal of egg-based and grass-based stains and/or soils, compositions and products comprising same |
| WO2005052146A2 (en) | 2003-11-19 | 2005-06-09 | Genencor International, Inc. | Serine proteases, nucleic acids encoding serine enzymes and vectors and host cells incorporating same |
| US7985569B2 (en) * | 2003-11-19 | 2011-07-26 | Danisco Us Inc. | Cellulomonas 69B4 serine protease variants |
| US20070281332A1 (en) | 2004-02-13 | 2007-12-06 | Allan Svendsen | Protease Variants |
| DK1751264T3 (da) | 2004-05-17 | 2010-05-03 | Procter & Gamble | Blegesammensætning, som omfatter af carbohydratoxidase |
| US20090060933A1 (en) * | 2004-06-14 | 2009-03-05 | Estell David A | Proteases producing an altered immunogenic response and methods of making and using the same |
| JP2006143855A (ja) * | 2004-11-18 | 2006-06-08 | Lion Corp | 飲料シミ汚れ除去効果を向上させたアミラーゼ含有漂白性組成物 |
| JP2006143853A (ja) * | 2004-11-18 | 2006-06-08 | Lion Corp | アミラーゼ含有漂白性組成物 |
| US8814861B2 (en) | 2005-05-12 | 2014-08-26 | Innovatech, Llc | Electrosurgical electrode and method of manufacturing same |
| US7147634B2 (en) | 2005-05-12 | 2006-12-12 | Orion Industries, Ltd. | Electrosurgical electrode and method of manufacturing same |
| JP2009500023A (ja) * | 2005-07-08 | 2009-01-08 | ノボザイムス アクティーゼルスカブ | サブチラーゼ変異体 |
| US20070161531A1 (en) * | 2005-07-08 | 2007-07-12 | Novozymes A/S | Subtilase variants |
| GB0603775D0 (en) * | 2006-02-24 | 2006-04-05 | Health Prot Agency | Infectivity assay |
| EP2007506B1 (en) * | 2006-03-31 | 2014-03-19 | Danisco US Inc. | Tangential flow filtration apparatuses, systems, and processes for the separation of compounds |
| US7629158B2 (en) | 2006-06-16 | 2009-12-08 | The Procter & Gamble Company | Cleaning and/or treatment compositions |
| JP5785686B2 (ja) | 2006-06-23 | 2015-09-30 | ダニスコ・ユーエス・インク、ジェネンコー・ディビジョン | 複数の性質をエンジニアリングするための部位評価ライブラリーを用いた、配列と活性との関係の系統的な評価 |
| RU2509152C2 (ru) * | 2006-07-18 | 2014-03-10 | ДАНИСКО ЮЭс, ИНК., ДЖЕНЕНКОР ДИВИЖН | Cпособ мытья посуды |
| AU2007284126B2 (en) | 2006-08-11 | 2013-12-19 | Novozymes Biologicals, Inc. | Bacteria cultures and compositions comprising bacteria cultures |
| WO2008048537A1 (en) * | 2006-10-16 | 2008-04-24 | Danisco Us, Inc. Genencor Division | Non-phosphate dish detergents |
| CN103667323B (zh) | 2007-03-12 | 2016-02-03 | 丹尼斯科美国公司 | 经修饰的蛋白酶 |
| US7922970B2 (en) * | 2007-04-03 | 2011-04-12 | Kimberly-Clark Worldwide, Inc. | Use of sonication to eliminate prions |
| WO2008141281A1 (en) | 2007-05-10 | 2008-11-20 | Danisco Us Inc., Genencor Division | A modified secretion system to increase expression of polypeptides in bacteria |
| KR20100028031A (ko) * | 2007-06-06 | 2010-03-11 | 다니스코 유에스 인크. | 단백질 특성의 개선 방법 |
| CA2699219C (en) * | 2007-09-12 | 2021-02-09 | Danisco Us Inc. | Filtration with internal fouling control |
| US8021436B2 (en) | 2007-09-27 | 2011-09-20 | The Procter & Gamble Company | Cleaning and/or treatment compositions comprising a xyloglucan conjugate |
| US7618801B2 (en) * | 2007-10-30 | 2009-11-17 | Danison US Inc. | Streptomyces protease |
| EP2215201A2 (en) | 2007-11-05 | 2010-08-11 | Danisco US Inc. | Alpha-amylase variants with altered properties |
| KR20100088675A (ko) | 2007-11-05 | 2010-08-10 | 다니스코 유에스 인크. | 변경된 특성을 지닌 바실러스 종 ｔｓ-23 알파-아밀라아제의 변이체 |
| CA2709804A1 (en) | 2007-12-21 | 2009-07-30 | Danisco Us Inc. | Enhanced protein production in bacillus |
| WO2009098229A2 (en) | 2008-02-04 | 2009-08-13 | Danisco Us Inc., Genencor Division | A method of preparing food products using ts23 alpha-amylase |
| US8066818B2 (en) * | 2008-02-08 | 2011-11-29 | The Procter & Gamble Company | Water-soluble pouch |
| EP2088187B2 (en) * | 2008-02-08 | 2020-01-22 | The Procter and Gamble Company | Process For Making A Water-Soluble Pouch |
| US20090209447A1 (en) * | 2008-02-15 | 2009-08-20 | Michelle Meek | Cleaning compositions |
| EP2100947A1 (en) | 2008-03-14 | 2009-09-16 | The Procter and Gamble Company | Automatic dishwashing detergent composition |
| EP2100948A1 (en) * | 2008-03-14 | 2009-09-16 | The Procter and Gamble Company | Automatic dishwashing detergent composition |
| US20090233830A1 (en) | 2008-03-14 | 2009-09-17 | Penny Sue Dirr | Automatic detergent dishwashing composition |
| EP2271660B1 (en) | 2008-03-26 | 2020-05-06 | Novozymes A/S | Stabilized liquid enzyme compositions |
| JP5659143B2 (ja) | 2008-03-28 | 2015-01-28 | ダニスコ・ユーエス・インク | 細菌性細胞中の遺伝子座を増幅する方法 |
| CN102057040A (zh) | 2008-06-06 | 2011-05-11 | 丹尼斯科美国公司 | 具有改良特性的嗜热脂肪土芽孢杆菌α-淀粉酶(AMYS)变体 |
| CA2726451A1 (en) * | 2008-06-06 | 2009-12-10 | Danisco Us Inc. | Compositions and methods comprising variant microbial proteases |
| EP2166092A1 (en) | 2008-09-18 | 2010-03-24 | The Procter and Gamble Company | Detergent composition |
| EP2166076A1 (en) * | 2008-09-23 | 2010-03-24 | The Procter & Gamble Company | Cleaning composition |
| EP2166073A1 (en) * | 2008-09-23 | 2010-03-24 | The Procter & Gamble Company | Cleaning composition |
| EP2166075A1 (en) * | 2008-09-23 | 2010-03-24 | The Procter and Gamble Company | Cleaning composition |
| CN102224234B (zh) | 2008-09-25 | 2014-04-16 | 丹尼斯科美国公司 | α-淀粉酶混合物和使用所述混合物的方法 |
| MX2011004803A (es) * | 2008-11-11 | 2011-06-16 | Danisco Inc | Composiciones y metodos que comprenden una variante de subtilisina. |
| AR074105A1 (es) | 2008-11-11 | 2010-12-22 | Danisco Us Inc | Composiciones y metodos que comprenden variantes de subtilisina |
| US8753861B2 (en) | 2008-11-11 | 2014-06-17 | Danisco Us Inc. | Protease comprising one or more combinable mutations |
| CN102209778B (zh) * | 2008-11-11 | 2014-10-15 | 丹尼斯科美国公司 | 包含丝氨酸蛋白酶变体的组合物和方法 |
| US20100267304A1 (en) * | 2008-11-14 | 2010-10-21 | Gregory Fowler | Polyurethane foam pad and methods of making and using same |
| US20100125046A1 (en) * | 2008-11-20 | 2010-05-20 | Denome Frank William | Cleaning products |
| EP2213715A1 (en) | 2009-02-02 | 2010-08-04 | The Procter & Gamble Company | Liquid hand dishwashing detergent composition |
| EP2213714B1 (en) | 2009-02-02 | 2014-06-11 | The Procter and Gamble Company | Liquid hand dishwashing detergent composition |
| EP2216392B1 (en) * | 2009-02-02 | 2013-11-13 | The Procter and Gamble Company | Liquid hand dishwashing detergent composition |
| EP2216391A1 (en) * | 2009-02-02 | 2010-08-11 | The Procter & Gamble Company | Liquid hand dishwashing detergent composition |
| ES2461892T3 (es) | 2009-02-02 | 2014-05-21 | The Procter & Gamble Company | Composición detergente líquida para lavado de vajillas a mano |
| EP2216390B1 (en) | 2009-02-02 | 2013-11-27 | The Procter and Gamble Company | Hand dishwashing method |
| EP2216393B1 (en) | 2009-02-09 | 2024-04-24 | The Procter & Gamble Company | Detergent composition |
| US8153574B2 (en) | 2009-03-18 | 2012-04-10 | The Procter & Gamble Company | Structured fluid detergent compositions comprising dibenzylidene polyol acetal derivatives and detersive enzymes |
| US8293697B2 (en) | 2009-03-18 | 2012-10-23 | The Procter & Gamble Company | Structured fluid detergent compositions comprising dibenzylidene sorbitol acetal derivatives |
| DK2414514T3 (en) * | 2009-04-01 | 2015-08-24 | Danisco Us Inc | FORMATIONS AND PROCEDURES COMPREHENSIVE Alpha- amylase variants WITH CHANGED PROPERTIES |
| WO2010138347A1 (en) | 2009-05-26 | 2010-12-02 | The Procter & Gamble Company | Aqueous liquid composition for pre-treating soiled dishware |
| AR076941A1 (es) | 2009-06-11 | 2011-07-20 | Danisco Us Inc | Cepa de bacillus para una mayor produccion de proteina |
| WO2011005917A1 (en) | 2009-07-09 | 2011-01-13 | The Procter & Gamble Company | Method of laundering fabric using a liquid laundry detergent composition |
| WO2011005913A1 (en) | 2009-07-09 | 2011-01-13 | The Procter & Gamble Company | A catalytic laundry detergent composition comprising relatively low levels of water-soluble electrolyte |
| WO2011005804A1 (en) | 2009-07-09 | 2011-01-13 | The Procter & Gamble Company | Method of laundering fabric using a liquid laundry detergent composition |
| US20110009307A1 (en) | 2009-07-09 | 2011-01-13 | Alan Thomas Brooker | Laundry Detergent Composition Comprising Low Level of Sulphate |
| CN102471729A (zh) | 2009-07-09 | 2012-05-23 | 宝洁公司 | 包含较低含量水溶性电解质的催化性衣物洗涤剂组合物 |
| WO2011005623A1 (en) | 2009-07-09 | 2011-01-13 | The Procter & Gamble Company | Laundry detergent composition comprising low level of bleach |
| BR112012000492A2 (pt) | 2009-07-09 | 2019-09-24 | Procter & Gamble | composição detergente moderadamente alcalina, com baixos teores de coadjuvantes que compreende ácido fitalimido peroxicaproico para tratamento de tecido sólido |
| EP2451922A1 (en) | 2009-07-09 | 2012-05-16 | The Procter & Gamble Company | Method of laundering fabric using a compacted liquid laundry detergent composition |
| JP5645937B2 (ja) | 2009-07-31 | 2014-12-24 | アクゾ ノーベル ナムローゼ フェンノートシャップＡｋｚｏ Ｎｏｂｅｌ Ｎ．Ｖ． | パーソナルケア用途のためのハイブリッドコポリマー組成物 |
| ES2581916T5 (es) | 2009-08-13 | 2022-11-07 | Procter & Gamble | Método para lavado de tejidos a baja temperatura |
| WO2011036263A1 (en) * | 2009-09-25 | 2011-03-31 | Novozymes A/S | Subtilase variants |
| JP2013506021A (ja) * | 2009-09-25 | 2013-02-21 | ノボザイムス アクティーゼルスカブ | 洗浄剤組成物 |
| US8728790B2 (en) | 2009-12-09 | 2014-05-20 | Danisco Us Inc. | Compositions and methods comprising protease variants |
| MX2012006616A (es) | 2009-12-09 | 2012-06-21 | Procter & Gamble | Productos para el cuidado de las telas y el hogar. |
| EP2333041B1 (en) | 2009-12-10 | 2013-05-15 | The Procter & Gamble Company | Method and use of a dishwasher composition |
| PL2333042T3 (pl) | 2009-12-10 | 2015-12-31 | Procter & Gamble | Produkt do automatycznych zmywarek do mycia naczyń i jego wykorzystanie |
| EP2333039B2 (en) | 2009-12-10 | 2020-11-11 | The Procter & Gamble Company | Method and use of a dishwasher composition |
| ES2399311T5 (es) | 2009-12-10 | 2020-06-19 | Procter & Gamble | Composición detergente |
| WO2011084417A1 (en) | 2009-12-21 | 2011-07-14 | Danisco Us Inc. | Detergent compositions containing geobacillus stearothermophilus lipase and methods of use thereof |
| BR112012017056A2 (pt) | 2009-12-21 | 2016-11-22 | Danisco Us Inc | "composições detergentes contendo lipase de bacillus subtilis e métodos para uso das mesmas" |
| CN102712879A (zh) | 2009-12-21 | 2012-10-03 | 丹尼斯科美国公司 | 含有褐色喜热裂孢菌脂肪酶的洗涤剂组合物及其使用方法 |
| US9045514B2 (en) * | 2010-01-22 | 2015-06-02 | Dupont Nutrition Biosciences Aps | Methods for producing amino-substituted glycolipid compounds |
| US9896673B2 (en) | 2010-02-10 | 2018-02-20 | Novozymes A/S | Compositions of high stability alpha amylase variants |
| EP2357220A1 (en) | 2010-02-10 | 2011-08-17 | The Procter & Gamble Company | Cleaning composition comprising amylase variants with high stability in the presence of a chelating agent |
| PL2361964T3 (pl) | 2010-02-25 | 2013-05-31 | Procter & Gamble | Kompozycja detergentu |
| EP2377914B1 (en) | 2010-04-19 | 2016-11-09 | The Procter & Gamble Company | Mildly alkaline, low-built, solid fabric treatment detergent composition comprising perhydrolase |
| EP2380957A1 (en) | 2010-04-19 | 2011-10-26 | The Procter & Gamble Company | Solid laundry detergent composition having a dynamic in-wash ph profile |
| EP2365059A1 (en) | 2010-03-01 | 2011-09-14 | The Procter & Gamble Company | Solid laundry detergent composition comprising C.I. fluorescent brightener 260 in alpha-crystalline form |
| EP2363456A1 (en) | 2010-03-01 | 2011-09-07 | The Procter & Gamble Company | Solid laundry detergent composition comprising brightener in micronized particulate form |
| EP2365058A1 (en) | 2010-03-01 | 2011-09-14 | The Procter & Gamble Company | Solid laundry detergent composition having an excellent anti-encrustation profile |
| EP2365054A1 (en) | 2010-03-01 | 2011-09-14 | The Procter & Gamble Company | Solid laundry detergent composition comprising secondary alcohol-based detersive surfactant |
| US20120067373A1 (en) * | 2010-04-15 | 2012-03-22 | Philip Frank Souter | Automatic Dishwashing Detergent Composition |
| WO2011130222A2 (en) | 2010-04-15 | 2011-10-20 | Danisco Us Inc. | Compositions and methods comprising variant proteases |
| US20110257069A1 (en) | 2010-04-19 | 2011-10-20 | Stephen Joseph Hodson | Detergent composition |
| US20110257062A1 (en) | 2010-04-19 | 2011-10-20 | Robert Richard Dykstra | Liquid laundry detergent composition comprising a source of peracid and having a ph profile that is controlled with respect to the pka of the source of peracid |
| US20110257060A1 (en) | 2010-04-19 | 2011-10-20 | Robert Richard Dykstra | Laundry detergent composition comprising bleach particles that are suspended within a continuous liquid phase |
| US8889612B2 (en) | 2010-04-19 | 2014-11-18 | The Procter & Gamble Company | Method of laundering fabric using a compacted liquid laundry detergent composition |
| EP2380478A1 (en) | 2010-04-23 | 2011-10-26 | The Procter & Gamble Company | Automatic dishwashing product |
| ES2682051T3 (es) | 2010-04-23 | 2018-09-18 | The Procter & Gamble Company | Composición detergente |
| ES2565192T3 (es) | 2010-04-23 | 2016-04-01 | The Procter & Gamble Company | Método para perfumar |
| ES2533368T3 (es) | 2010-04-23 | 2015-04-09 | The Procter & Gamble Company | Producto para lavavajillas |
| EP2383329A1 (en) | 2010-04-23 | 2011-11-02 | The Procter & Gamble Company | Particle |
| EP2380962B1 (en) | 2010-04-23 | 2016-03-30 | The Procter and Gamble Company | Particle |
| EP3095861B1 (en) | 2010-05-06 | 2019-06-26 | The Procter and Gamble Company | Consumer products with protease variants |
| DE102010028951A1 (de) | 2010-05-12 | 2011-11-17 | Henkel Ag & Co. Kgaa | Lagerstabiles flüssiges Wasch- oder Reinigungsmittel enthaltend Protease und Lipase |
| AR081423A1 (es) | 2010-05-28 | 2012-08-29 | Danisco Us Inc | Composiciones detergentes con contenido de lipasa de streptomyces griseus y metodos para utilizarlas |
| EP2395071A1 (en) | 2010-06-10 | 2011-12-14 | The Procter & Gamble Company | Solid detergent composition comprising lipase of bacterial origin |
| EP2395070A1 (en) | 2010-06-10 | 2011-12-14 | The Procter & Gamble Company | Liquid laundry detergent composition comprising lipase of bacterial origin |
| EP2585573A1 (en) | 2010-06-23 | 2013-05-01 | The Procter and Gamble Company | Product for pre-treatment and laundering of stained fabric |
| EP2412792A1 (en) | 2010-07-29 | 2012-02-01 | The Procter & Gamble Company | Liquid detergent composition |
| US8685171B2 (en) | 2010-07-29 | 2014-04-01 | The Procter & Gamble Company | Liquid detergent composition |
| CN101922108B (zh) * | 2010-09-14 | 2012-09-05 | 东华大学 | 使用1，4，7-三氮杂环壬烷配合物的活化漂白的方法 |
| WO2012057781A1 (en) | 2010-10-29 | 2012-05-03 | The Procter & Gamble Company | Cleaning and/or treatment compositions comprising a fungal serine protease |
| WO2011026154A2 (en) | 2010-10-29 | 2011-03-03 | The Procter & Gamble Company | Cleaning and/or treatment compositions |
| FI123942B (fi) | 2010-10-29 | 2013-12-31 | Ab Enzymes Oy | Sieniperäisen seriiniproteaasin variantteja |
| DE102010063458A1 (de) * | 2010-12-17 | 2012-06-21 | Henkel Ag & Co. Kgaa | Lagerstabiles flüssiges Wasch- oder Reinigungsmittel enthaltend Protease und Amylase |
| WO2012112718A1 (en) | 2011-02-15 | 2012-08-23 | Novozymes Biologicals, Inc. | Mitigation of odor in cleaning machines and cleaning processes |
| DE102011005354A1 (de) * | 2011-03-10 | 2012-09-13 | Henkel Ag & Co. Kgaa | Leistungsverbesserte Proteasevarianten |
| US8802388B2 (en) | 2011-04-29 | 2014-08-12 | Danisco Us Inc. | Detergent compositions containing Bacillus agaradhaerens mannanase and methods of use thereof |
| EP2712363A1 (en) | 2011-04-29 | 2014-04-02 | Danisco US Inc. | Detergent compositions containing geobacillus tepidamans mannanase and methods of use thereof |
| WO2012149333A1 (en) | 2011-04-29 | 2012-11-01 | Danisco Us Inc. | Detergent compositions containing bacillus sp. mannanase and methods of use thereof |
| CN103764823B (zh) | 2011-05-05 | 2018-05-11 | 丹尼斯科美国公司 | 包含丝氨酸蛋白酶变体的组合物和方法 |
| JP5933688B2 (ja) * | 2011-05-05 | 2016-06-15 | ザ プロクター アンド ギャンブル カンパニー | セリンプロテアーゼ変異体を含む組成物及び方法 |
| US20140371435A9 (en) | 2011-06-03 | 2014-12-18 | Eduardo Torres | Laundry Care Compositions Containing Thiophene Azo Dyes |
| EP2537918A1 (en) | 2011-06-20 | 2012-12-26 | The Procter & Gamble Company | Consumer products with lipase comprising coated particles |
| US20120324655A1 (en) | 2011-06-23 | 2012-12-27 | Nalini Chawla | Product for pre-treatment and laundering of stained fabric |
| EP2540824A1 (en) | 2011-06-30 | 2013-01-02 | The Procter & Gamble Company | Cleaning compositions comprising amylase variants reference to a sequence listing |
| ES2834102T3 (es) | 2011-06-30 | 2021-06-16 | Novozymes As | Variantes de alfa-amilasa |
| EP2551335A1 (en) | 2011-07-25 | 2013-01-30 | The Procter & Gamble Company | Enzyme stabilized liquid detergent composition |
| US20130029894A1 (en) | 2011-07-27 | 2013-01-31 | Jean-Luc Philippe Bettiol | Multiphase liquid detergent composition |
| US8679366B2 (en) | 2011-08-05 | 2014-03-25 | Ecolab Usa Inc. | Cleaning composition containing a polysaccharide graft polymer composition and methods of controlling hard water scale |
| US8841246B2 (en) | 2011-08-05 | 2014-09-23 | Ecolab Usa Inc. | Cleaning composition containing a polysaccharide hybrid polymer composition and methods of improving drainage |
| US8636918B2 (en) | 2011-08-05 | 2014-01-28 | Ecolab Usa Inc. | Cleaning composition containing a polysaccharide hybrid polymer composition and methods of controlling hard water scale |
| US8853144B2 (en) | 2011-08-05 | 2014-10-07 | Ecolab Usa Inc. | Cleaning composition containing a polysaccharide graft polymer composition and methods of improving drainage |
| AR087745A1 (es) | 2011-08-31 | 2014-04-16 | Danisco Us Inc | Composiciones y metodos que comprenden una variante de enzima lipolitica |
| PL2584028T3 (pl) | 2011-10-19 | 2017-10-31 | Procter & Gamble | Cząstka |
| IN2014DN03123A (pl) | 2011-11-04 | 2015-05-22 | Akzo Nobel Chemicals Int Bv | |
| BR112014009040A2 (pt) | 2011-11-04 | 2017-05-09 | Akzo Nobel Chemicals Int Bv | copolímero obtenível através da polimerização de pelo menos um primeiro monômero etilenicamente não saturado e pelo menos um segundo monômero etilenicamente não saturado; composição de copolímero; e processo para preparação do copolímero de dendrito |
| WO2013086219A1 (en) | 2011-12-09 | 2013-06-13 | Danisco Us Inc. | Ribosomal promotors from b. subtilis for protein production in microorganisms |
| RU2014128577A (ru) | 2011-12-13 | 2016-02-10 | ДАНИСКО ЮЭс ИНК. | Ферментные смеси, полученные из смешанных культур |
| CN104011204A (zh) * | 2011-12-20 | 2014-08-27 | 诺维信公司 | 枯草杆菌酶变体和编码它们的多核苷酸 |
| US20150017700A1 (en) | 2011-12-22 | 2015-01-15 | Danisco Us Inc. | Compositions and methods comprising a lipolytic enzyme variant |
| BR112014017919A2 (pt) | 2012-01-26 | 2017-06-27 | Novozymes As | uso de um poliptídeo variante, composição, polinucleotídeo isolado, constructo de ácido nucleico ou vetor de expressão, célula hospedeira de expressão recombinante, métodos de produção do pilipeptídeo, para melhoria do valor nutricional de uma ração animal, e para o tratamento de proteínas, planta, parte de planta ou célula de planta transgênica, aditivo de ração animal, ração animal, e, composição de ração animal ou detergente |
| MX353896B (es) | 2012-02-03 | 2018-02-01 | Procter & Gamble | Composiciones y metodos para el tratamiento de superficies con lipasas. |
| CA2867714A1 (en) | 2012-03-19 | 2013-09-26 | The Procter & Gamble Company | Laundry care compositions containing dyes |
| CN107988181A (zh) | 2012-04-02 | 2018-05-04 | 诺维信公司 | 脂肪酶变体以及编码其的多核苷酸 |
| DK2847308T3 (en) | 2012-05-07 | 2017-10-23 | Novozymes As | Polypeptides with xanthan-degrading activity and polynucleotides encoding them |
| US20150164755A1 (en) * | 2012-05-30 | 2015-06-18 | Clariant Finance (Bvi0 Limited | Use Of N-Methyl-N-Acylglucamines As Cold Stabilizers In Surfactant Solutions |
| EP2674475A1 (en) | 2012-06-11 | 2013-12-18 | The Procter & Gamble Company | Detergent composition |
| AU2013279440B2 (en) | 2012-06-20 | 2016-10-06 | Novozymes A/S | Use of polypeptides having protease activity in animal feed and detergents |
| WO2014009473A1 (en) | 2012-07-12 | 2014-01-16 | Novozymes A/S | Polypeptides having lipase activity and polynucleotides encoding same |
| US8945314B2 (en) | 2012-07-30 | 2015-02-03 | Ecolab Usa Inc. | Biodegradable stability binding agent for a solid detergent |
| US9994799B2 (en) | 2012-09-13 | 2018-06-12 | Ecolab Usa Inc. | Hard surface cleaning compositions comprising phosphinosuccinic acid adducts and methods of use |
| US9752105B2 (en) | 2012-09-13 | 2017-09-05 | Ecolab Usa Inc. | Two step method of cleaning, sanitizing, and rinsing a surface |
| US20140308162A1 (en) | 2013-04-15 | 2014-10-16 | Ecolab Usa Inc. | Peroxycarboxylic acid based sanitizing rinse additives for use in ware washing |
| US8871699B2 (en) | 2012-09-13 | 2014-10-28 | Ecolab Usa Inc. | Detergent composition comprising phosphinosuccinic acid adducts and methods of use |
| EP2712915A1 (en) | 2012-10-01 | 2014-04-02 | The Procter and Gamble Company | Methods of treating a surface and compositions for use therein |
| CA2885774C (en) | 2012-10-04 | 2019-04-02 | Ecolab Usa Inc. | Pre-soak technology for laundry and other hard surface cleaning |
| HK1209785A1 (en) | 2012-10-12 | 2016-04-08 | Danisco Us Inc. | Compositions and methods comprising a lipolytic enzyme variant |
| KR20150082502A (ko) | 2012-11-05 | 2015-07-15 | 다니스코 유에스 인크. | 써몰리신 프로테아제 변이체를 포함하는 조성물 및 방법 |
| US20150344858A1 (en) | 2012-12-19 | 2015-12-03 | Danisco Us Inc. | Novel mannanase, compositions and methods of use thereof |
| MX2015007802A (es) | 2012-12-20 | 2015-09-04 | Procter & Gamble | Composiciones de detergente con blanqueador recubierto con silicato. |
| EP3354728B1 (en) | 2012-12-21 | 2020-04-22 | Danisco US Inc. | Alpha-amylase variants |
| WO2014099525A1 (en) | 2012-12-21 | 2014-06-26 | Danisco Us Inc. | Paenibacillus curdlanolyticus amylase, and methods of use, thereof |
| HUE052331T2 (hu) | 2013-02-19 | 2021-04-28 | Procter & Gamble | Textiltisztítási eljárás |
| EP2767582A1 (en) | 2013-02-19 | 2014-08-20 | The Procter and Gamble Company | Method of laundering a fabric |
| EP2767579B1 (en) | 2013-02-19 | 2018-07-18 | The Procter and Gamble Company | Method of laundering a fabric |
| EP2964740B1 (en) | 2013-03-05 | 2017-10-04 | The Procter and Gamble Company | Mixed sugar-based amide surfactant compositions |
| DK2970931T3 (en) | 2013-03-11 | 2018-01-22 | Danisco Us Inc | COMBINATORY ALPHA AMYLASE VARIATIONS |
| US20160068620A1 (en) | 2013-03-15 | 2016-03-10 | Lubrizol Advanced Materials, Inc. | Itaconic Acid Polymers |
| US9631164B2 (en) | 2013-03-21 | 2017-04-25 | Novozymes A/S | Polypeptides with lipase activity and polynucleotides encoding same |
| US10577564B2 (en) | 2013-03-28 | 2020-03-03 | The Procter & Gamble Company | Cleaning compositions containing a polyetheramine |
| CN103215151B (zh) * | 2013-04-25 | 2015-01-07 | 上海巴方精细化工有限公司 | 矢车菊低刺激护肤香皂 |
| US20160083703A1 (en) | 2013-05-17 | 2016-03-24 | Novozymes A/S | Polypeptides having alpha amylase activity |
| JP6188199B2 (ja) * | 2013-05-20 | 2017-08-30 | ライオン株式会社 | 食器洗い機用洗浄剤 |
| CN103361198B (zh) * | 2013-05-22 | 2015-01-07 | 上海巴方精细化工有限公司 | 川芎消炎复合香皂 |
| JP6077177B2 (ja) | 2013-05-28 | 2017-02-08 | ザ プロクター アンド ギャンブル カンパニー | フォトクロミック染料を含む表面処理組成物 |
| DK3882346T3 (da) | 2013-05-29 | 2025-12-22 | Danisco Us Inc | Hidtil ukendte metalloproteaser |
| EP3004342B1 (en) | 2013-05-29 | 2023-01-11 | Danisco US Inc. | Novel metalloproteases |
| WO2014194034A2 (en) | 2013-05-29 | 2014-12-04 | Danisco Us Inc. | Novel metalloproteases |
| WO2014194032A1 (en) | 2013-05-29 | 2014-12-04 | Danisco Us Inc. | Novel metalloproteases |
| US10378001B2 (en) | 2013-06-27 | 2019-08-13 | Novozymes A/S | Subtilase variants and compositions comprising same |
| US20160152925A1 (en) | 2013-07-04 | 2016-06-02 | Novozymes A/S | Polypeptides Having Anti-Redeposition Effect and Polynucleotides Encoding Same |
| WO2015010009A2 (en) | 2013-07-19 | 2015-01-22 | Danisco Us Inc. | Compositions and methods comprising a lipolytic enzyme variant |
| MX2016001317A (es) * | 2013-07-29 | 2016-04-07 | Danisco Us Inc | Variantes de enzimas. |
| CN117904081A (zh) * | 2013-07-29 | 2024-04-19 | 诺维信公司 | 蛋白酶变体以及对其进行编码的多核苷酸 |
| WO2015038792A1 (en) | 2013-09-12 | 2015-03-19 | Danisco Us Inc. | Compositions and methods comprising lg12-clade protease variants |
| WO2015042013A1 (en) | 2013-09-18 | 2015-03-26 | Lubrizol Advanced Materials, Inc. | Stable linear polymers |
| US9834682B2 (en) | 2013-09-18 | 2017-12-05 | Milliken & Company | Laundry care composition comprising carboxylate dye |
| CN105555935A (zh) | 2013-09-18 | 2016-05-04 | 宝洁公司 | 包含羧化物染料的衣物洗涤护理组合物 |
| MX2016003538A (es) | 2013-09-18 | 2016-06-28 | Procter & Gamble | Composiciones para el cuidado de la ropa que contienen colorantes azoicos de tiofeno y carboxilato. |
| WO2015042086A1 (en) | 2013-09-18 | 2015-03-26 | The Procter & Gamble Company | Laundry care composition comprising carboxylate dye |
| DE102013219467A1 (de) * | 2013-09-26 | 2015-03-26 | Henkel Ag & Co. Kgaa | Stabilisierte Inhibitor-Proteasevarianten |
| EP2857487A1 (en) | 2013-10-07 | 2015-04-08 | WeylChem Switzerland AG | Multi-compartment pouch comprising cleaning compositions, washing process and use for washing and cleaning of textiles and dishes |
| EP2857485A1 (en) | 2013-10-07 | 2015-04-08 | WeylChem Switzerland AG | Multi-compartment pouch comprising alkanolamine-free cleaning compositions, washing process and use for washing and cleaning of textiles and dishes |
| EP2857486A1 (en) | 2013-10-07 | 2015-04-08 | WeylChem Switzerland AG | Multi-compartment pouch comprising cleaning compositions, washing process and use for washing and cleaning of textiles and dishes |
| EP3058055A1 (en) | 2013-10-15 | 2016-08-24 | Danisco US Inc. | Clay granule |
| DK3068879T3 (da) | 2013-11-14 | 2020-03-16 | Danisco Us Inc | Stabile enzymer opnået ved glykeringsreduktion |
| US20160312204A1 (en) | 2013-12-13 | 2016-10-27 | Danisco Us Inc. | Serine proteases of bacillus species |
| EP3080263B1 (en) | 2013-12-13 | 2019-07-03 | Danisco US Inc. | Serine proteases of the bacillus gibsonii-clade |
| US10005850B2 (en) | 2013-12-16 | 2018-06-26 | E I Du Pont De Nemours And Company | Use of poly alpha-1,3-glucan ethers as viscosity modifiers |
| US9957334B2 (en) | 2013-12-18 | 2018-05-01 | E I Du Pont De Nemours And Company | Cationic poly alpha-1,3-glucan ethers |
| EP3089991B1 (en) | 2013-12-31 | 2019-08-28 | Danisco US Inc. | Enhanced protein expression |
| WO2015112340A1 (en) | 2014-01-22 | 2015-07-30 | The Procter & Gamble Company | Method of treating textile fabrics |
| EP3097172A1 (en) | 2014-01-22 | 2016-11-30 | The Procter & Gamble Company | Method of treating textile fabrics |
| EP3097173B1 (en) | 2014-01-22 | 2020-12-23 | The Procter and Gamble Company | Fabric treatment composition |
| EP3097175B1 (en) | 2014-01-22 | 2018-10-17 | The Procter and Gamble Company | Fabric treatment composition |
| CA2841024C (en) | 2014-01-30 | 2017-03-07 | The Procter & Gamble Company | Unit dose article |
| WO2015123323A1 (en) | 2014-02-14 | 2015-08-20 | E. I. Du Pont De Nemours And Company | Poly-alpha-1,3-1,6-glucans for viscosity modification |
| US10752562B2 (en) | 2014-02-25 | 2020-08-25 | The Procter & Gamble Company | Process for making renewable surfactant intermediates and surfactants from fats and oils and products thereof |
| US9994497B2 (en) | 2014-02-25 | 2018-06-12 | The Procter & Gamble Company | Process for making renewable surfactant intermediates and surfactants from fats and oils and products thereof |
| JP6367976B2 (ja) | 2014-02-26 | 2018-08-01 | 信越化学工業株式会社 | 消泡剤組成物 |
| EP2915873A1 (en) * | 2014-03-06 | 2015-09-09 | The Procter and Gamble Company | Dishwashing composition |
| EP3116914B8 (en) | 2014-03-11 | 2021-04-21 | E. I. du Pont de Nemours and Company | Oxidized poly alpha-1,3-glucan as detergent builder |
| BR112016021154A2 (pt) | 2014-03-14 | 2021-08-24 | Lubrizol Advanced Materials, Inc. | Polímeros e copolímeros de ácido itacônico |
| MX2016012044A (es) | 2014-03-21 | 2017-06-29 | Danisco Us Inc | Serina proteasas de especies de bacillus. |
| EP3122850A1 (en) | 2014-03-27 | 2017-02-01 | The Procter & Gamble Company | Cleaning compositions containing a polyetheramine |
| CA2941253A1 (en) | 2014-03-27 | 2015-10-01 | Frank Hulskotter | Cleaning compositions containing a polyetheramine |
| EP2924105A1 (en) | 2014-03-28 | 2015-09-30 | The Procter and Gamble Company | Water soluble unit dose article |
| EP2924106A1 (en) | 2014-03-28 | 2015-09-30 | The Procter and Gamble Company | Water soluble unit dose article |
| US20170015950A1 (en) | 2014-04-01 | 2017-01-19 | Novozymes A/S | Polypeptides having alpha amylase activity |
| EP3140384B1 (en) | 2014-05-06 | 2024-02-14 | Milliken & Company | Laundry care compositions |
| US9365805B2 (en) | 2014-05-15 | 2016-06-14 | Ecolab Usa Inc. | Bio-based pot and pan pre-soak |
| EP3152288A1 (en) | 2014-06-06 | 2017-04-12 | The Procter & Gamble Company | Detergent composition comprising polyalkyleneimine polymers |
| US20170121695A1 (en) | 2014-06-12 | 2017-05-04 | Novozymes A/S | Alpha-amylase variants and polynucleotides encoding same |
| US9714403B2 (en) | 2014-06-19 | 2017-07-25 | E I Du Pont De Nemours And Company | Compositions containing one or more poly alpha-1,3-glucan ether compounds |
| EP3919599A1 (en) | 2014-06-19 | 2021-12-08 | Nutrition & Biosciences USA 4, Inc. | Compositions containing one or more poly alpha-1,3-glucan ether compounds |
| CN106661566A (zh) | 2014-07-04 | 2017-05-10 | 诺维信公司 | 枯草杆菌酶变体以及编码它们的多核苷酸 |
| EP3739029A1 (en) | 2014-07-04 | 2020-11-18 | Novozymes A/S | Subtilase variants and polynucleotides encoding same |
| BR112017002158B1 (pt) | 2014-08-01 | 2022-03-15 | Ecolab Usa Inc | Método de limpeza manual de superfície usando produtos têxteis de limpeza e sistema |
| EP2987848A1 (en) | 2014-08-19 | 2016-02-24 | The Procter & Gamble Company | Method of laundering a fabric |
| EP2987849A1 (en) | 2014-08-19 | 2016-02-24 | The Procter and Gamble Company | Method of Laundering a Fabric |
| CN106687572A (zh) | 2014-09-10 | 2017-05-17 | 巴斯夫欧洲公司 | 包封清洁组合物 |
| US9617502B2 (en) | 2014-09-15 | 2017-04-11 | The Procter & Gamble Company | Detergent compositions containing salts of polyetheramines and polymeric acid |
| JP6396583B2 (ja) | 2014-09-25 | 2018-09-26 | ザ プロクター アンド ギャンブル カンパニー | ポリエーテルアミンを含有する洗浄組成物 |
| US20160090552A1 (en) | 2014-09-25 | 2016-03-31 | The Procter & Gamble Company | Detergent compositions containing a polyetheramine and an anionic soil release polymer |
| US9388368B2 (en) | 2014-09-26 | 2016-07-12 | The Procter & Gamble Company | Cleaning compositions containing a polyetheramine |
| DK3207129T3 (da) | 2014-10-17 | 2020-02-24 | Danisco Us Inc | Serinproteaser af bacillus-arten |
| EP3172329A1 (en) | 2014-10-24 | 2017-05-31 | Danisco US Inc. | Method for producing alcohol by use of a tripeptidyl peptidase |
| WO2016069569A2 (en) | 2014-10-27 | 2016-05-06 | Danisco Us Inc. | Serine proteases |
| CN107148472A (zh) | 2014-10-27 | 2017-09-08 | 丹尼斯科美国公司 | 芽孢杆菌属物种的丝氨酸蛋白酶 |
| DK3212662T3 (da) | 2014-10-27 | 2020-07-20 | Danisco Us Inc | Serinproteaser |
| EP3212781B1 (en) | 2014-10-27 | 2019-09-18 | Danisco US Inc. | Serine proteases |
| EP3212783B1 (en) | 2014-10-27 | 2024-06-26 | Danisco US Inc. | Serine proteases |
| EP3256563A1 (en) | 2014-11-17 | 2017-12-20 | The Procter and Gamble Company | Benefit agent delivery compositions |
| US10287562B2 (en) * | 2014-11-20 | 2019-05-14 | Novoszymes A/S | Alicyclobacillus variants and polynucleotides encoding same |
| KR102588719B1 (ko) | 2014-12-16 | 2023-10-12 | 다니스코 유에스 인크. | 향상된 단백질 발현 |
| WO2016100128A1 (en) | 2014-12-19 | 2016-06-23 | Danisco Us Inc | Enhanced protein expression |
| CN107109450A (zh) | 2014-12-23 | 2017-08-29 | 纳幕尔杜邦公司 | 酶促产生的纤维素 |
| EP3259358B1 (en) | 2015-02-19 | 2024-06-19 | Danisco US Inc. | Enhanced protein expression |
| DK3268471T3 (da) | 2015-03-12 | 2019-12-02 | Danisco Us Inc | Sammensætninger og fremgangsmåder omfattende lg12-clade-proteasevarianter |
| EP3088504B1 (en) | 2015-04-29 | 2021-07-21 | The Procter & Gamble Company | Method of treating a fabric |
| EP3088506B1 (en) | 2015-04-29 | 2018-05-23 | The Procter and Gamble Company | Detergent composition |
| WO2016176280A1 (en) | 2015-04-29 | 2016-11-03 | The Procter & Gamble Company | Method of treating a fabric |
| CN107624127A (zh) | 2015-04-29 | 2018-01-23 | 宝洁公司 | 处理织物的方法 |
| HUE043043T2 (hu) | 2015-04-29 | 2019-07-29 | Procter & Gamble | Textilkezelési módszer |
| WO2016178668A1 (en) | 2015-05-04 | 2016-11-10 | Milliken & Company | Leuco triphenylmethane colorants as bluing agents in laundry care compositions |
| CA2981409A1 (en) | 2015-05-08 | 2016-11-17 | Novozymes A/S | Alpha-amylase variants and polynucleotides encoding same |
| US20180142224A1 (en) | 2015-05-08 | 2018-05-24 | Novozymes A/S | Alpha-amylase variants having improved performance and stability |
| WO2016180749A1 (en) | 2015-05-08 | 2016-11-17 | Novozymes A/S | Alpha-amylase variants and polynucleotides encoding same |
| EP4219704A3 (en) | 2015-05-13 | 2023-08-23 | Danisco US Inc | Aprl-clade protease variants and uses thereof |
| EP3303536B1 (en) | 2015-06-02 | 2019-04-17 | Unilever PLC | Laundry detergent composition |
| EP3307427B1 (en) | 2015-06-09 | 2023-08-16 | Danisco US Inc. | Osmotic burst encapsulates |
| WO2016198262A1 (en) | 2015-06-11 | 2016-12-15 | Unilever Plc | Laundry detergent composition |
| JP7015695B2 (ja) | 2015-06-17 | 2022-02-03 | ダニスコ・ユーエス・インク | バチルス・ギブソニイ（Ｂａｃｉｌｌｕｓ ｇｉｂｓｏｎｉｉ）クレードセリンプロテアーゼ |
| EP3313966B1 (en) | 2015-06-26 | 2020-07-29 | Unilever PLC | Laundry detergent composition |
| CN108350443B (zh) | 2015-09-17 | 2022-06-28 | 诺维信公司 | 具有黄原胶降解活性的多肽以及编码它们的多核苷酸 |
| CN108026487B (zh) | 2015-09-17 | 2021-04-30 | 汉高股份有限及两合公司 | 包含具有黄原胶降解活性的多肽的洗涤剂组合物 |
| BR112018007096A2 (pt) * | 2015-10-09 | 2018-10-23 | Novozymes As | método de lavagem de roupa, uso de composição detergente e polipeptídeo |
| MX388896B (es) | 2015-10-28 | 2025-03-20 | Novozymes As | Composicion detergente que comprende variantes de amilasa y proteasa. |
| CN108603183B (zh) | 2015-11-05 | 2023-11-03 | 丹尼斯科美国公司 | 类芽孢杆菌属物种和芽孢杆菌属物种甘露聚糖酶 |
| EP3371308B1 (en) | 2015-11-05 | 2022-05-11 | Danisco US Inc. | Paenibacillus sp. mannanases |
| WO2017083228A1 (en) | 2015-11-13 | 2017-05-18 | E. I. Du Pont De Nemours And Company | Glucan fiber compositions for use in laundry care and fabric care |
| EP3374488B1 (en) | 2015-11-13 | 2020-10-14 | DuPont Industrial Biosciences USA, LLC | Glucan fiber compositions for use in laundry care and fabric care |
| WO2017083229A1 (en) | 2015-11-13 | 2017-05-18 | E. I. Du Pont De Nemours And Company | Glucan fiber compositions for use in laundry care and fabric care |
| WO2017089093A1 (en) * | 2015-11-25 | 2017-06-01 | Unilever N.V. | A liquid detergent composition |
| EP3387124B1 (en) | 2015-12-09 | 2021-05-19 | Danisco US Inc. | Alpha-amylase combinatorial variants |
| JP6591278B2 (ja) * | 2015-12-15 | 2019-10-16 | 花王株式会社 | 食器用固体洗浄剤組成物 |
| US20180362946A1 (en) | 2015-12-18 | 2018-12-20 | Danisco Us Inc. | Polypeptides with endoglucanase activity and uses thereof |
| JP7626576B2 (ja) | 2015-12-30 | 2025-02-07 | ノボザイムス アクティーゼルスカブ | 酵素変異体およびこれをコードするポリヌクレオチド |
| EP3205392A1 (en) | 2016-02-12 | 2017-08-16 | Basf Se | Microcapsules and process for preparation of microcapsules |
| EP3205393A1 (en) | 2016-02-12 | 2017-08-16 | Basf Se | Process for preparation of microcapsules |
| EP3417039B1 (en) | 2016-02-17 | 2019-07-10 | Unilever PLC | Whitening composition |
| EP3417040B1 (en) | 2016-02-17 | 2019-09-04 | Unilever PLC | Whitening composition |
| WO2017152169A1 (en) | 2016-03-04 | 2017-09-08 | Danisco Us Inc. | Engineered ribosomal promoters for protein production in microorganisms |
| US20190085273A1 (en) | 2016-03-09 | 2019-03-21 | Basf Se | Encapsulated laundry cleaning composition |
| CN108884415A (zh) | 2016-03-21 | 2018-11-23 | 荷兰联合利华有限公司 | 洗衣洗涤剂组合物 |
| CN108779416B (zh) | 2016-04-08 | 2021-01-05 | 荷兰联合利华有限公司 | 洗衣洗涤剂组合物 |
| WO2017182295A1 (en) | 2016-04-18 | 2017-10-26 | Basf Se | Liquid cleaning compositions |
| BR112018072530A2 (pt) * | 2016-05-03 | 2019-03-26 | Danisco Us Inc | variantes de protease e usos das mesmas |
| BR112018072586A2 (pt) | 2016-05-05 | 2019-02-19 | Danisco Us Inc | variantes de protease e usos das mesmas |
| JP6067168B1 (ja) * | 2016-05-30 | 2017-01-25 | 株式会社ニイタカ | 自動洗浄機用洗浄剤組成物 |
| CA3026064A1 (en) | 2016-05-31 | 2017-12-07 | Danisco Us Inc. | Protease variants and uses thereof |
| CN110218712B (zh) * | 2016-06-02 | 2021-08-03 | 天津科技大学 | 磷脂酶d突变体及其在制备磷脂酸、磷脂酰丝氨酸中的应用 |
| CN109312323B (zh) * | 2016-06-09 | 2022-03-08 | 花王株式会社 | 突变碱性蛋白酶 |
| CA3027745A1 (en) | 2016-06-17 | 2017-12-21 | Danisco Us Inc. | Protease variants and uses thereof |
| EP3275985A1 (en) | 2016-07-26 | 2018-01-31 | The Procter and Gamble Company | Automatic dishwashing detergent composition |
| EP3275989A1 (en) | 2016-07-26 | 2018-01-31 | The Procter and Gamble Company | Automatic dishwashing detergent composition |
| EP3275987A1 (en) | 2016-07-26 | 2018-01-31 | The Procter and Gamble Company | Automatic dishwashing detergent composition |
| EP3275988B1 (en) | 2016-07-26 | 2020-07-08 | The Procter and Gamble Company | Automatic dishwashing detergent composition |
| EP3275986B1 (en) | 2016-07-26 | 2020-07-08 | The Procter and Gamble Company | Automatic dishwashing detergent composition |
| US11512300B2 (en) | 2016-08-24 | 2022-11-29 | Novozymes A/S | Xanthan lyase variants and polynucleotides encoding same |
| KR102483218B1 (ko) | 2016-08-24 | 2023-01-02 | 헨켈 아게 운트 코. 카게아아 | 크산탄 리아제 변이체 i을 포함하는 세제 조성물 |
| US10988747B2 (en) | 2016-08-24 | 2021-04-27 | Henkel Ag & Co. Kgaa | Detergent composition comprising GH9 endoglucanase variants I |
| CA3031609A1 (en) | 2016-08-24 | 2018-03-01 | Novozymes A/S | Gh9 endoglucanase variants and polynucleotides encoding same |
| JP6790257B2 (ja) | 2016-11-01 | 2020-11-25 | ザ プロクター アンド ギャンブル カンパニーＴｈｅ Ｐｒｏｃｔｅｒ ＆ Ｇａｍｂｌｅ Ｃｏｍｐａｎｙ | 洗濯ケア組成物における青味剤としてのロイコ着色剤、その包装、キット及び方法 |
| US10851329B2 (en) | 2016-11-01 | 2020-12-01 | Milliken & Company | Leuco colorants as bluing agents in laundry care compositions |
| WO2018085310A1 (en) | 2016-11-01 | 2018-05-11 | The Procter & Gamble Company | Leuco colorants as bluing agents in laundry care compositions |
| US20180119056A1 (en) | 2016-11-03 | 2018-05-03 | Milliken & Company | Leuco Triphenylmethane Colorants As Bluing Agents in Laundry Care Compositions |
| WO2018085524A2 (en) | 2016-11-07 | 2018-05-11 | Danisco Us Inc | Laundry detergent composition |
| US10577571B2 (en) | 2016-11-08 | 2020-03-03 | Ecolab Usa Inc. | Non-aqueous cleaner for vegetable oil soils |
| CN110088261B (zh) | 2016-12-02 | 2022-05-06 | 宝洁公司 | 包含酶的清洁组合物 |
| CN110023476B (zh) | 2016-12-02 | 2021-07-06 | 宝洁公司 | 包含酶的清洁组合物 |
| US10550443B2 (en) | 2016-12-02 | 2020-02-04 | The Procter & Gamble Company | Cleaning compositions including enzymes |
| EP3555255B1 (en) | 2016-12-15 | 2020-06-24 | Unilever PLC | Laundry detergent composition |
| US11946081B2 (en) | 2016-12-21 | 2024-04-02 | Danisco Us Inc. | Bacillus gibsonii-clade serine proteases |
| EP4520820A3 (en) | 2016-12-21 | 2025-04-16 | Danisco Us Inc | Protease variants and uses thereof |
| EP3339423A1 (en) | 2016-12-22 | 2018-06-27 | The Procter & Gamble Company | Automatic dishwashing detergent composition |
| CN110234747B (zh) | 2017-02-01 | 2021-11-09 | 宝洁公司 | 包含淀粉酶变体的清洁组合物 |
| EP3585910B1 (en) | 2017-02-24 | 2024-03-20 | Danisco US Inc. | Compositions and methods for increased protein production in bacillus licheniformis |
| EP3583210B1 (en) | 2017-03-15 | 2021-07-07 | Danisco US Inc. | Trypsin-like serine proteases and uses thereof |
| DK3601553T3 (da) | 2017-03-31 | 2026-02-16 | Danisco Us Inc | Alfa-amylase-kombinationsvarianter |
| US20200040283A1 (en) | 2017-03-31 | 2020-02-06 | Danisco Us Inc | Delayed release enzyme formulations for bleach-containing detergents |
| WO2018206535A1 (en) | 2017-05-08 | 2018-11-15 | Novozymes A/S | Carbohydrate-binding domain and polynucleotides encoding the same |
| EP3401385A1 (en) | 2017-05-08 | 2018-11-14 | Henkel AG & Co. KGaA | Detergent composition comprising polypeptide comprising carbohydrate-binding domain |
| CA3067837A1 (en) | 2017-06-30 | 2019-01-03 | Danisco Us Inc | Low-agglomeration, enzyme-containing particles |
| BR112020000205B1 (pt) | 2017-07-07 | 2023-10-31 | Unilever Ip Holdings B.V. | Composição de limpeza para a lavagem de tecidos e método doméstico de tratamento de um tecido |
| CN110869480B (zh) | 2017-07-07 | 2021-08-13 | 联合利华知识产权控股有限公司 | 增白组合物 |
| EP3668973A2 (en) | 2017-08-18 | 2020-06-24 | Danisco US Inc. | Alpha-amylase variants |
| US11879127B2 (en) | 2017-08-23 | 2024-01-23 | Danisco Us Inc. | Methods and compositions for efficient genetic modifications of Bacillus licheniformis strains |
| US11624059B2 (en) | 2017-08-24 | 2023-04-11 | Henkel Ag & Co. Kgaa | Detergent compositions comprising GH9 endoglucanase variants II |
| WO2019038057A1 (en) | 2017-08-24 | 2019-02-28 | Novozymes A/S | VARIANTS OF XANTHANE LYASE AND POLYNUCLEOTIDES ENCODING THE SAME |
| US11525128B2 (en) | 2017-08-24 | 2022-12-13 | Novozymes A/S | GH9 endoglucanase variants and polynucleotides encoding same |
| US20210130744A1 (en) | 2017-08-24 | 2021-05-06 | Henkel Ag & Co. Kgaa | Detergent composition comprising xanthan lyase variants ii |
| WO2019055261A1 (en) | 2017-09-13 | 2019-03-21 | Danisco Us Inc | MODIFIED 5 'NON-TRANSLATED REGION (UTR) SEQUENCES FOR INCREASED PROTEIN PRODUCTION IN BACILLUS |
| TWI715878B (zh) | 2017-10-12 | 2021-01-11 | 美商美力肯及公司 | 隱色著色劑及組成物 |
| WO2019075144A1 (en) | 2017-10-12 | 2019-04-18 | The Procter & Gamble Company | LEUCO COLORANTS IN COMBINATION WITH A SECOND BLEACHING AGENT AS AZURING AGENTS IN LAUNDRY CARE COMPOSITIONS |
| US20190194579A1 (en) | 2017-10-12 | 2019-06-27 | The Procter & Gamble Company | Leuco colorants as bluing agents in laundry care compositions |
| JP6993503B2 (ja) | 2017-10-12 | 2022-01-13 | ザ プロクター アンド ギャンブル カンパニー | 洗濯ケア組成物中の青味剤としてのロイコ着色剤 |
| WO2019089228A1 (en) | 2017-11-01 | 2019-05-09 | Milliken & Company | Leuco compounds, colorant compounds, and compositions containing the same |
| EP3703661A1 (en) | 2017-11-02 | 2020-09-09 | Danisco US Inc. | Freezing point depressed solid matrix compositions for melt granulation of enzymes |
| EP3717643A1 (en) | 2017-11-29 | 2020-10-07 | Danisco US Inc. | Subtilisin variants having improved stability |
| WO2019105675A1 (en) | 2017-11-30 | 2019-06-06 | Unilever Plc | Detergent composition comprising protease |
| DK3502246T3 (da) | 2017-12-19 | 2025-07-07 | Procter & Gamble | Rensemiddelsammensætning til automatisk opvask |
| EP3502244B1 (en) | 2017-12-19 | 2025-03-05 | The Procter & Gamble Company | Automatic dishwashing detergent composition |
| EP3502227B1 (en) | 2017-12-19 | 2024-09-04 | The Procter & Gamble Company | Automatic dishwashing detergent composition |
| EP3502245B1 (en) | 2017-12-19 | 2025-03-05 | The Procter & Gamble Company | Automatic dishwashing detergent composition |
| DE102017223281A1 (de) * | 2017-12-19 | 2019-06-19 | Henkel Ag & Co. Kgaa | Reinigugnsmittel enthaltend durch Betain stabilisierte Amylase |
| EP3728543B1 (en) | 2017-12-21 | 2025-10-29 | Danisco US Inc. | Enzyme-containing, hot-melt granules comprising a thermotolerant desiccant |
| JP6967447B2 (ja) * | 2017-12-27 | 2021-11-17 | 小林製薬株式会社 | 錠剤状義歯洗浄剤 |
| JP7280269B2 (ja) | 2018-01-03 | 2023-05-23 | ダニスコ・ユーエス・インク | タンパク質の産生増加のための変異及び遺伝子改変バチルス属（ｂａｃｉｌｌｕｓ）細胞、並びにその方法 |
| CN111556891B (zh) | 2018-01-26 | 2021-11-05 | 宝洁公司 | 包含酶的水溶性单位剂量制品 |
| US20200359656A1 (en) | 2018-02-08 | 2020-11-19 | Danisco Us Inc. | Thermally-resistant wax matrix particles for enzyme encapsulation |
| EP3755793A1 (en) | 2018-02-23 | 2020-12-30 | Henkel AG & Co. KGaA | Detergent composition comprising xanthan lyase and endoglucanase variants |
| WO2019168650A1 (en) | 2018-02-28 | 2019-09-06 | The Procter & Gamble Company | Methods of cleaning |
| JP2021513590A (ja) | 2018-02-28 | 2021-05-27 | ザ プロクター アンド ギャンブル カンパニーＴｈｅ Ｐｒｏｃｔｅｒ ＆ Ｇａｍｂｌｅ Ｃｏｍｐａｎｙ | 洗浄組成物 |
| WO2019180111A1 (en) * | 2018-03-23 | 2019-09-26 | Novozymes A/S | Subtilase variants and compositions comprising same |
| BR112020023083A2 (pt) | 2018-05-17 | 2021-02-02 | Unilever N.V. | composição de limpeza fluida, composição detergente líquida para lavanderia e uso de uma combinação de tensoativos |
| JP2021527413A (ja) | 2018-06-19 | 2021-10-14 | ザ プロクター アンド ギャンブル カンパニーＴｈｅ Ｐｒｏｃｔｅｒ ＆ Ｇａｍｂｌｅ Ｃｏｍｐａｎｙ | 自動食器洗浄洗剤組成物 |
| EP3799601A1 (en) | 2018-06-19 | 2021-04-07 | Danisco US Inc. | Subtilisin variants |
| US11220656B2 (en) | 2018-06-19 | 2022-01-11 | The Procter & Gamble Company | Automatic dishwashing detergent composition |
| WO2019245704A1 (en) | 2018-06-19 | 2019-12-26 | Danisco Us Inc | Subtilisin variants |
| US20200032178A1 (en) | 2018-07-27 | 2020-01-30 | The Procter & Gamble Company | Water-soluble unit dose articles comprising water-soluble fibrous structures and particles |
| US12584119B2 (en) | 2018-07-31 | 2026-03-24 | Danisco Us Inc | Variant alpha-amylases having amino acid substitutions that lower the PKA of the general acid |
| EP3844255A1 (en) | 2018-08-30 | 2021-07-07 | Danisco US Inc. | Enzyme-containing granules |
| WO2020046613A1 (en) | 2018-08-30 | 2020-03-05 | Danisco Us Inc | Compositions comprising a lipolytic enzyme variant and methods of use thereof |
| BR112021004507A2 (pt) | 2018-09-17 | 2021-06-08 | Unilever Ip Holdings B.V. | composição detergente, método de tratamento de um substrato com uma composição detergente e uso de uma enzima lipase bacteriana |
| JP2022503923A (ja) | 2018-09-27 | 2022-01-12 | ダニスコ・ユーエス・インク | 医療用器具を洗浄するための組成物 |
| WO2020077331A2 (en) | 2018-10-12 | 2020-04-16 | Danisco Us Inc | Alpha-amylases with mutations that improve stability in the presence of chelants |
| EP3884023B1 (en) | 2018-11-20 | 2024-07-17 | Unilever Global Ip Limited | Detergent composition |
| BR112021009785A2 (pt) | 2018-11-20 | 2021-08-17 | Unilever Ip Holdings B.V. | composição detergente, método de tratamento de um substrato de tecido e uso de uma enzima |
| EP3884025B1 (en) | 2018-11-20 | 2022-06-08 | Unilever Global Ip Limited | Detergent composition |
| EP3884022B1 (en) | 2018-11-20 | 2024-06-12 | Unilever Global Ip Limited | Detergent composition |
| CN113056550B (zh) | 2018-11-20 | 2022-10-28 | 联合利华知识产权控股有限公司 | 洗涤剂组合物 |
| EP3887515A1 (en) | 2018-11-28 | 2021-10-06 | Danisco US Inc. | Subtilisin variants having improved stability |
| CN113330101A (zh) | 2018-12-21 | 2021-08-31 | 诺维信公司 | 包含金属蛋白酶的洗涤剂袋 |
| EP3702452A1 (en) * | 2019-03-01 | 2020-09-02 | Novozymes A/S | Detergent compositions comprising two proteases |
| CN113597469A (zh) | 2019-03-14 | 2021-11-02 | 宝洁公司 | 包含酶的清洁组合物 |
| EP3938503A1 (en) | 2019-03-14 | 2022-01-19 | The Procter & Gamble Company | Method for treating cotton |
| CN113439116B (zh) | 2019-03-14 | 2023-11-28 | 宝洁公司 | 包含酶的清洁组合物 |
| AU2020242303B2 (en) | 2019-03-21 | 2026-01-08 | Novozymes A/S | Alpha-amylase variants and polynucleotides encoding same |
| CN113825829A (zh) | 2019-05-16 | 2021-12-21 | 联合利华知识产权控股有限公司 | 洗衣组合物 |
| WO2020229661A1 (en) | 2019-05-16 | 2020-11-19 | Unilever Plc | Laundry composition |
| US11261404B2 (en) | 2019-05-20 | 2022-03-01 | Ecolab Usa Inc. | Surfactant package for high foaming detergents with low level of medium to long chain linear alcohols |
| ES3028186T3 (en) | 2019-05-22 | 2025-06-18 | Procter & Gamble | Automatic dishwashing method |
| WO2020243738A1 (en) | 2019-05-24 | 2020-12-03 | The Procter & Gamble Company | Automatic dishwashing detergent composition |
| CN114174504B (zh) | 2019-05-24 | 2026-04-17 | 丹尼斯科美国公司 | 枯草杆菌蛋白酶变体和使用方法 |
| WO2020247582A1 (en) | 2019-06-06 | 2020-12-10 | Danisco Us Inc | Methods and compositions for cleaning |
| MX2021015382A (es) | 2019-06-24 | 2022-01-24 | Procter & Gamble | Composiciones de limpieza que comprenden variantes de amilasa. |
| WO2020259949A1 (en) | 2019-06-28 | 2020-12-30 | Unilever Plc | Detergent composition |
| CN114008184B (zh) | 2019-06-28 | 2024-12-06 | 联合利华知识产权控股有限公司 | 洗涤剂组合物 |
| WO2020260006A1 (en) | 2019-06-28 | 2020-12-30 | Unilever Plc | Detergent compositions |
| BR112021025261A2 (pt) | 2019-06-28 | 2022-04-26 | Unilever Ip Holdings B V | Composição detergente e método doméstico para tratar um tecido |
| US20220364020A1 (en) | 2019-06-28 | 2022-11-17 | Conopco, Inc., D/B/A Unilever | Detergent composition |
| CN113891930A (zh) | 2019-06-28 | 2022-01-04 | 联合利华知识产权控股有限公司 | 洗涤剂组合物 |
| EP3770241A1 (de) * | 2019-07-22 | 2021-01-27 | Henkel AG & Co. KGaA | Reinigungsmittel mit protease zur automatischen dosierung |
| US11873465B2 (en) | 2019-08-14 | 2024-01-16 | Ecolab Usa Inc. | Methods of cleaning and soil release of highly oil absorbing substrates employing optimized extended chain nonionic surfactants |
| CN114364776A (zh) | 2019-09-02 | 2022-04-15 | 联合利华知识产权控股有限公司 | 洗涤剂组合物 |
| US20220372399A1 (en) | 2019-09-19 | 2022-11-24 | Conopco, Inc., D/B/A Unilever | Detergent compositions |
| AR120142A1 (es) | 2019-10-07 | 2022-02-02 | Unilever Nv | Composición detergente |
| US11492571B2 (en) | 2019-10-24 | 2022-11-08 | The Procter & Gamble Company | Automatic dishwashing detergent composition comprising a protease |
| US12410385B2 (en) | 2019-10-24 | 2025-09-09 | The Procter & Gamble Company | Automatic dishwashing detergent composition comprising an amylase |
| EP4048683A2 (en) | 2019-10-24 | 2022-08-31 | Danisco US Inc | Variant maltopentaose/maltohexaose-forming alpha-amylases |
| KR102316445B1 (ko) * | 2019-11-29 | 2021-10-26 | 씨제이제일제당 주식회사 | 신규 세린 프로테아제 변이체 |
| EP3835396B1 (en) | 2019-12-09 | 2025-12-03 | The Procter & Gamble Company | A detergent composition comprising a polymer |
| US20230049452A1 (en) | 2020-01-13 | 2023-02-16 | Danisco Us Inc | Compositions comprising a lipolytic enzyme variant and methods of use thereof |
| CN114945665A (zh) | 2020-01-15 | 2022-08-26 | 丹尼斯科美国公司 | 用于增强地衣芽孢杆菌中蛋白产生的组合物和方法 |
| WO2021151536A1 (en) | 2020-01-29 | 2021-08-05 | Unilever Ip Holdings B.V. | Laundry detergent product |
| EP3862412A1 (en) | 2020-02-04 | 2021-08-11 | The Procter & Gamble Company | Detergent composition |
| MX2022013878A (es) | 2020-06-05 | 2022-11-30 | Procter & Gamble | Composiciones detergentes que contienen un surfactante ramificado. |
| EP4162018B1 (en) | 2020-06-08 | 2024-01-31 | Unilever IP Holdings B.V. | Method of improving protease activity |
| CA3185062A1 (en) | 2020-07-06 | 2022-01-13 | Gang Pu | Foaming mixed alcohol/water compositions comprising a structured alkoxylated siloxane |
| CN116075583A (zh) | 2020-07-06 | 2023-05-05 | 埃科莱布美国股份有限公司 | 包含烷基硅氧烷和水溶助剂/增溶剂的组合的发泡混合醇/水组合物 |
| EP4176031A1 (en) | 2020-07-06 | 2023-05-10 | Ecolab USA Inc. | Peg-modified castor oil based compositions for microemulsifying and removing multiple oily soils |
| WO2022031309A1 (en) | 2020-08-04 | 2022-02-10 | The Procter & Gamble Company | Automatic dishwashing method |
| JP7708845B2 (ja) | 2020-08-04 | 2025-07-15 | ザ プロクター アンド ギャンブル カンパニー | 自動食器洗浄方法及びパック |
| CA3187725A1 (en) | 2020-08-04 | 2022-02-10 | The Procter & Gamble Company | Automatic dishwashing method |
| CA3186847A1 (en) | 2020-08-04 | 2022-02-10 | Nevena Lukic | Automatic dishwashing method |
| US20240034960A1 (en) | 2020-08-27 | 2024-02-01 | Danisco Us Inc | Enzymes and enzyme compositions for cleaning |
| US20230287302A1 (en) | 2020-08-28 | 2023-09-14 | Conopco, Inc., D/B/A Unilever | Detergent composition |
| US20230265408A1 (en) * | 2020-08-28 | 2023-08-24 | Novozymes A/S | Protease variants with improved solubility |
| BR112023002979A2 (pt) | 2020-08-28 | 2023-04-04 | Unilever Ip Holdings B V | Composição detergente e método de tratamento de um artigo têxtil |
| CN116157496A (zh) | 2020-08-28 | 2023-05-23 | 联合利华知识产权控股有限公司 | 表面活性剂和洗涤剂组合物 |
| WO2022043042A1 (en) | 2020-08-28 | 2022-03-03 | Unilever Ip Holdings B.V. | Detergent composition |
| BR112023001773A2 (pt) | 2020-08-28 | 2023-03-28 | Unilever Ip Holdings B V | Composição detergente e método |
| CN116507725A (zh) | 2020-10-07 | 2023-07-28 | 诺维信公司 | α-淀粉酶变体 |
| WO2022093189A1 (en) | 2020-10-27 | 2022-05-05 | Milliken & Company | Compositions comprising leuco compounds and colorants |
| WO2022094589A1 (en) | 2020-10-29 | 2022-05-05 | The Procter & Gamble Company | Cleaning compositions containing alginase enzymes |
| EP4001387A1 (en) | 2020-11-17 | 2022-05-25 | The Procter & Gamble Company | Automatic dishwashing composition commprising amphiphilic graft polymer |
| JP7667265B2 (ja) | 2020-11-17 | 2025-04-22 | ザ プロクター アンド ギャンブル カンパニー | アルカリ性すすぎによる自動食器洗浄方法 |
| EP4001388A1 (en) | 2020-11-17 | 2022-05-25 | The Procter & Gamble Company | Automatic dishwashing method with amphiphilic graft polymer in the rinse |
| EP4006131A1 (en) | 2020-11-30 | 2022-06-01 | The Procter & Gamble Company | Method of laundering fabric |
| WO2022122481A1 (en) | 2020-12-07 | 2022-06-16 | Unilever Ip Holdings B.V. | Detergent compositions |
| WO2022122480A1 (en) | 2020-12-07 | 2022-06-16 | Unilever Ip Holdings B.V. | Detergent compositions |
| CN116529285B (zh) | 2020-12-23 | 2025-05-13 | 巴斯夫股份公司 | 两亲性烷氧基化多胺及其用途 |
| CN112662652A (zh) * | 2021-01-20 | 2021-04-16 | 天津科技大学 | 一种胶原降解活力降低的碱性蛋白酶突变体 |
| EP4284906A1 (en) | 2021-01-29 | 2023-12-06 | Danisco US Inc. | Compositions for cleaning and methods related thereto |
| EP4039806A1 (en) | 2021-02-04 | 2022-08-10 | Henkel AG & Co. KGaA | Detergent composition comprising xanthan lyase and endoglucanase variants with im-proved stability |
| EP4291646A2 (en) | 2021-02-12 | 2023-12-20 | Novozymes A/S | Alpha-amylase variants |
| KR102613549B1 (ko) | 2021-03-12 | 2023-12-13 | 씨제이제일제당 주식회사 | 신규 세린 프로테아제 변이체 |
| CN116615536A (zh) | 2021-03-15 | 2023-08-18 | 宝洁公司 | 含有多肽变体的清洁组合物 |
| CA3211422A1 (en) | 2021-05-05 | 2022-11-10 | Neil Joseph Lant | Methods for making cleaning compositions and detecting soils |
| EP4086330A1 (en) | 2021-05-06 | 2022-11-09 | The Procter & Gamble Company | Surface treatment |
| WO2022243367A1 (en) | 2021-05-18 | 2022-11-24 | Nouryon Chemicals International B.V. | Polyester polyquats in cleaning applications |
| WO2022243533A1 (en) | 2021-05-20 | 2022-11-24 | Nouryon Chemicals International B.V. | Manufactured polymers having altered oligosaccharide or polysaccharide functionality or narrowed oligosaccharide distribution, processes for preparing them, compositions containing them, and methods of using them |
| EP4108767A1 (en) | 2021-06-22 | 2022-12-28 | The Procter & Gamble Company | Cleaning or treatment compositions containing nuclease enzymes |
| EP4359518A1 (en) | 2021-06-23 | 2024-05-01 | Novozymes A/S | Alpha-amylase polypeptides |
| WO2023278297A1 (en) | 2021-06-30 | 2023-01-05 | Danisco Us Inc | Variant lipases and uses thereof |
| US20240287409A1 (en) | 2021-06-30 | 2024-08-29 | Nouryon Chemicals International B.V. | Chelate-amphoteric surfactant liquid concentrates and use thereof in cleaning applications |
| EP4123007B1 (en) | 2021-07-19 | 2026-04-08 | The Procter & Gamble Company | Fabric treatment using bacterial spores |
| EP4123006B1 (en) | 2021-07-19 | 2026-03-25 | The Procter & Gamble Company | Composition comprising spores and pro-perfume materials |
| WO2023017794A1 (ja) | 2021-08-10 | 2023-02-16 | 株式会社日本触媒 | ポリアルキレンオキシド含有化合物 |
| US20240352499A1 (en) | 2021-08-20 | 2024-10-24 | Danisco Us Inc. | Polynucleotides encoding novel nucleases, compositions thereof and methods thereof for eliminating dna from protein preparations |
| EP4396320A2 (en) | 2021-09-03 | 2024-07-10 | Danisco US Inc. | Laundry compositions for cleaning |
| EP4402258A2 (en) | 2021-09-13 | 2024-07-24 | Danisco US Inc. | Bioactive-containing granules |
| WO2023041694A1 (en) | 2021-09-20 | 2023-03-23 | Unilever Ip Holdings B.V. | Detergent composition |
| WO2023057367A1 (en) | 2021-10-08 | 2023-04-13 | Unilever Ip Holdings B.V. | Laundry composition |
| EP4416255A1 (en) | 2021-10-14 | 2024-08-21 | The Procter & Gamble Company | A fabric and home care product comprising cationic soil release polymer and lipase enzyme |
| EP4194537A1 (en) | 2021-12-08 | 2023-06-14 | The Procter & Gamble Company | Laundry treatment cartridge |
| EP4194536A1 (en) | 2021-12-08 | 2023-06-14 | The Procter & Gamble Company | Laundry treatment cartridge |
| JP7842216B2 (ja) | 2021-12-16 | 2026-04-07 | ザ プロクター アンド ギャンブル カンパニー | プロテアーゼを含む自動食器洗浄組成物 |
| WO2023114793A1 (en) | 2021-12-16 | 2023-06-22 | The Procter & Gamble Company | Home care composition |
| WO2023114988A2 (en) | 2021-12-16 | 2023-06-22 | Danisco Us Inc. | Variant maltopentaose/maltohexaose-forming alpha-amylases |
| EP4448750A2 (en) | 2021-12-16 | 2024-10-23 | Danisco US Inc. | Subtilisin variants and uses thereof |
| WO2023114939A2 (en) | 2021-12-16 | 2023-06-22 | Danisco Us Inc. | Subtilisin variants and methods of use |
| CA3240638A1 (en) | 2021-12-16 | 2023-06-22 | Michelle Jackson | Fabric and home care composition comprising a protease |
| CN118369413A (zh) | 2021-12-16 | 2024-07-19 | 宝洁公司 | 包含淀粉酶的家庭护理组合物 |
| EP4448751A2 (en) | 2021-12-16 | 2024-10-23 | Danisco US Inc. | Subtilisin variants and methods of use |
| WO2023168234A1 (en) | 2022-03-01 | 2023-09-07 | Danisco Us Inc. | Enzymes and enzyme compositions for cleaning |
| EP4273209A1 (en) | 2022-05-04 | 2023-11-08 | The Procter & Gamble Company | Machine-cleaning compositions containing enzymes |
| EP4273210A1 (en) | 2022-05-04 | 2023-11-08 | The Procter & Gamble Company | Detergent compositions containing enzymes |
| CN119630280A (zh) | 2022-05-14 | 2025-03-14 | 诺维信公司 | 用于预防、处理、抑制和/或消除植物病原性侵染和感染的组合物和方法 |
| EP4279571A1 (en) | 2022-05-19 | 2023-11-22 | The Procter & Gamble Company | Laundry composition comprising spores |
| WO2023227335A1 (en) | 2022-05-27 | 2023-11-30 | Unilever Ip Holdings B.V. | Liquid composition comprising linear alkyl benzene sulphonate, methyl ester ethoxylate and alkoxylated zwitterionic polyamine polymer |
| WO2023227332A1 (en) | 2022-05-27 | 2023-11-30 | Unilever Ip Holdings B.V. | Laundry liquid composition comprising a surfactant, an alkoxylated zwitterionic polyamine polymer and a protease |
| CN119365577A (zh) | 2022-05-27 | 2025-01-24 | 联合利华知识产权控股有限公司 | 包含酶的组合物 |
| CN119213107A (zh) | 2022-05-27 | 2024-12-27 | 联合利华知识产权控股有限公司 | 包含表面活性剂、烷氧基化两性离子聚胺聚合物和芳香剂的洗衣液体组合物 |
| WO2023227331A1 (en) | 2022-05-27 | 2023-11-30 | Unilever Ip Holdings B.V. | Composition comprising a specific methyl ester ethoxylate surfactant and a lipase |
| CN119301230A (zh) | 2022-05-27 | 2025-01-10 | 联合利华知识产权控股有限公司 | 包含表面活性剂、氨基羧酸盐、有机酸和香料的洗衣液体组合物 |
| EP4532671B1 (en) | 2022-06-03 | 2026-02-11 | Unilever IP Holdings B.V. | Laundry detergent product |
| CN119487167A (zh) | 2022-06-21 | 2025-02-18 | 丹尼斯科美国公司 | 用于清洁的包含具有嗜热菌蛋白酶活性的多肽的组合物和方法 |
| WO2024020445A1 (en) | 2022-07-20 | 2024-01-25 | Ecolab Usa Inc. | Novel nonionic extended surfactants, compositions and methods of use thereof |
| EP4321604A1 (en) | 2022-08-08 | 2024-02-14 | The Procter & Gamble Company | A fabric and home care composition comprising surfactant and a polyester |
| EP4581137A1 (en) | 2022-09-02 | 2025-07-09 | Danisco Us Inc | Mannanase variants and methods of use |
| EP4581119A1 (en) | 2022-09-02 | 2025-07-09 | Danisco US Inc. | Detergent compositions and methods related thereto |
| CN120112635A (zh) | 2022-09-02 | 2025-06-06 | 丹尼斯科美国公司 | 枯草杆菌蛋白酶变体及其相关方法 |
| US20260078316A1 (en) | 2022-09-13 | 2026-03-19 | Conopco, Inc., D/B/A Unilever | Washing machine and washing method |
| WO2024056333A1 (en) | 2022-09-13 | 2024-03-21 | Unilever Ip Holdings B.V. | Washing machine and washing method |
| WO2024056332A1 (en) | 2022-09-13 | 2024-03-21 | Unilever Ip Holdings B.V. | Washing machine and washing method |
| EP4587542A1 (en) | 2022-09-13 | 2025-07-23 | Unilever IP Holdings B.V. | Washing machine and washing method |
| EP4349944A1 (en) | 2022-10-05 | 2024-04-10 | Unilever IP Holdings B.V. | Laundry liquid composition |
| EP4349942A1 (en) | 2022-10-05 | 2024-04-10 | Unilever IP Holdings B.V. | Laundry liquid composition |
| EP4349948A1 (en) | 2022-10-05 | 2024-04-10 | Unilever IP Holdings B.V. | Laundry liquid composition |
| EP4349945A1 (en) | 2022-10-05 | 2024-04-10 | Unilever IP Holdings B.V. | Laundry liquid composition |
| EP4349943A1 (en) | 2022-10-05 | 2024-04-10 | Unilever IP Holdings B.V. | Laundry liquid composition |
| EP4349947A1 (en) | 2022-10-05 | 2024-04-10 | Unilever IP Holdings B.V. | Laundry liquid composition |
| EP4349946A1 (en) | 2022-10-05 | 2024-04-10 | Unilever IP Holdings B.V. | Unit dose fabric treatment product |
| WO2024088706A1 (en) | 2022-10-25 | 2024-05-02 | Unilever Ip Holdings B.V. | Composition |
| EP4608955A1 (en) | 2022-10-25 | 2025-09-03 | Unilever IP Holdings B.V. | Composition |
| EP4361239A1 (en) | 2022-10-25 | 2024-05-01 | Unilever IP Holdings B.V. | Laundry liquid composition |
| EP4612209A1 (en) | 2022-11-04 | 2025-09-10 | The Procter & Gamble Company | Fabric and home care composition |
| WO2024094785A1 (en) | 2022-11-04 | 2024-05-10 | Clariant International Ltd | Polyesters |
| WO2024094778A1 (en) | 2022-11-04 | 2024-05-10 | Clariant International Ltd | Polyesters |
| CN120303400A (zh) | 2022-11-09 | 2025-07-11 | 丹尼斯科美国公司 | 枯草杆菌蛋白酶变体和使用方法 |
| EP4627028A1 (en) | 2022-11-29 | 2025-10-08 | Unilever IP Holdings B.V. | Composition |
| EP4630526A1 (en) | 2022-12-05 | 2025-10-15 | The Procter & Gamble Company | Fabric and home care composition comprising a polyalkylenecarbonate compound |
| EP4634352A1 (en) | 2022-12-12 | 2025-10-22 | The Procter & Gamble Company | Fabric and home care composition |
| EP4386074B1 (en) | 2022-12-16 | 2025-12-03 | The Procter & Gamble Company | Fabric and home care composition |
| EP4388967A1 (en) | 2022-12-19 | 2024-06-26 | The Procter & Gamble Company | Dishwashing method |
| WO2024131880A2 (en) | 2022-12-23 | 2024-06-27 | Novozymes A/S | Detergent composition comprising catalase and amylase |
| WO2024163584A1 (en) | 2023-02-01 | 2024-08-08 | Danisco Us Inc. | Subtilisin variants and methods of use |
| JP2026503673A (ja) | 2023-02-01 | 2026-01-29 | ザ プロクター アンド ギャンブル カンパニー | 酵素を含有する洗剤組成物 |
| EP4677080A1 (en) | 2023-03-06 | 2026-01-14 | Danisco US Inc. | Subtilisin variants and methods of use |
| CN120813242A (zh) | 2023-03-16 | 2025-10-17 | 营养与生物科学美国第四公司 | 用于清洁和臭味控制的短芽孢杆菌发酵物提取物及其用途 |
| WO2024194190A1 (en) | 2023-03-17 | 2024-09-26 | Unilever Ip Holdings B.V. | Composition |
| EP4680708A1 (en) | 2023-03-17 | 2026-01-21 | Unilever IP Holdings B.V. | Machine dishwash filter cleaner |
| WO2024194098A1 (en) | 2023-03-21 | 2024-09-26 | Unilever Ip Holdings B.V. | Detergent unit dose |
| EP4695363A1 (en) | 2023-04-11 | 2026-02-18 | Unilever IP Holdings B.V. | Composition |
| EP4695360A1 (en) | 2023-04-11 | 2026-02-18 | Unilever IP Holdings B.V. | Composition |
| WO2024213430A1 (en) | 2023-04-11 | 2024-10-17 | Unilever Ip Holdings B.V. | Composition |
| EP4695364A1 (en) | 2023-04-11 | 2026-02-18 | Unilever IP Holdings B.V. | Composition |
| EP4695362A1 (en) | 2023-04-11 | 2026-02-18 | Unilever IP Holdings B.V. | Composition |
| WO2024223218A1 (en) | 2023-04-25 | 2024-10-31 | Unilever Ip Holdings B.V. | Composition |
| EP4458932A1 (en) | 2023-05-04 | 2024-11-06 | The Procter & Gamble Company | A fabric and home care composition |
| EP4458933A1 (en) | 2023-05-05 | 2024-11-06 | The Procter & Gamble Company | A fabric and home care composition comprising a propoxylated polyol |
| EP4481027A1 (en) | 2023-06-19 | 2024-12-25 | The Procter & Gamble Company | Cleaning compositions containing enzymes |
| EP4484536A1 (en) | 2023-06-26 | 2025-01-01 | The Procter & Gamble Company | Fabric and home care composition |
| EP4488351A1 (en) | 2023-07-03 | 2025-01-08 | The Procter & Gamble Company | Compositions containing a porphyrin binding protein |
| EP4662299A1 (en) | 2023-07-11 | 2025-12-17 | Unilever IP Holdings B.V. | Method for treating fabric |
| PL4662298T1 (pl) | 2023-07-11 | 2026-01-12 | Unilever Ip Holdings B.V. | Sposób traktowania materiału włókienniczego |
| CN121488025A (zh) | 2023-07-13 | 2026-02-06 | 联合利华知识产权控股有限公司 | 洗衣机和方法 |
| WO2025016669A1 (en) | 2023-07-19 | 2025-01-23 | Unilever Ip Holdings B.V. | Laundry capsule |
| CN121773188A (zh) | 2023-08-02 | 2026-03-31 | 联合利华知识产权控股有限公司 | 组合物 |
| WO2025031752A1 (en) | 2023-08-04 | 2025-02-13 | Unilever Ip Holdings B.V. | Composition |
| CN121729476A (zh) | 2023-08-04 | 2026-03-24 | 联合利华知识产权控股有限公司 | 组合物 |
| EP4509589A1 (en) | 2023-08-16 | 2025-02-19 | Unilever IP Holdings B.V. | Unit dose product |
| WO2025071996A1 (en) | 2023-09-28 | 2025-04-03 | Danisco Us Inc. | Variant cutinase enzymes with improved solubility and uses thereof |
| WO2025085351A1 (en) | 2023-10-20 | 2025-04-24 | Danisco Us Inc. | Subtilisin variants and methods of use |
| EP4549541A1 (en) | 2023-11-02 | 2025-05-07 | The Procter & Gamble Company | Fabric and home care composition |
| EP4549540A1 (en) | 2023-11-02 | 2025-05-07 | The Procter & Gamble Company | Fabric and home care composition |
| EP4553137A1 (en) | 2023-11-08 | 2025-05-14 | The Procter & Gamble Company | A fabric and home care composition comprising a polyester |
| EP4559998A1 (en) | 2023-11-22 | 2025-05-28 | The Procter & Gamble Company | Composition comprising microcapsules comprising spores |
| DE102023212361A1 (de) * | 2023-12-07 | 2025-06-12 | Henkel Ag & Co. Kgaa | Protease-varianten mit verbesserter bleichestabilität |
| WO2025124811A1 (en) | 2023-12-14 | 2025-06-19 | Unilever Ip Holdings B.V. | Composition |
| EP4570890A1 (en) | 2023-12-14 | 2025-06-18 | Unilever IP Holdings B.V. | Composition |
| EP4570892A1 (en) | 2023-12-15 | 2025-06-18 | The Procter & Gamble Company | A laundry detergent composition |
| EP4570893A1 (en) | 2023-12-15 | 2025-06-18 | The Procter & Gamble Company | Fabric and home care composition |
| WO2025153645A1 (en) | 2024-01-18 | 2025-07-24 | Unilever Ip Holdings B.V. | Use for fabric shape retention |
| WO2025153644A1 (en) | 2024-01-18 | 2025-07-24 | Unilever Ip Holdings B.V. | Composition |
| EP4610340A1 (en) | 2024-03-01 | 2025-09-03 | The Procter & Gamble Company | A laundry detergent composition comprising a polyester |
| EP4624555A1 (en) | 2024-03-26 | 2025-10-01 | The Procter & Gamble Company | Fabric and home care compositions |
| EP4624554A1 (en) | 2024-03-26 | 2025-10-01 | The Procter & Gamble Company | Fabric care compositions |
| WO2025202379A1 (en) | 2024-03-27 | 2025-10-02 | Basf Se | Polypeptides having protease activity for use in detergent compositions |
| EP4624572A1 (en) | 2024-03-27 | 2025-10-01 | Basf Se | Polypeptides having protease activity for use in detergent compositions |
| WO2025202382A1 (en) | 2024-03-27 | 2025-10-02 | Basf Se | Polypeptides having protease activity for use in cleaning compositions |
| WO2025202374A1 (en) | 2024-03-27 | 2025-10-02 | Basf Se | Polypeptides having protease activity for use in detergent compositions |
| WO2025202372A1 (en) | 2024-03-27 | 2025-10-02 | Basf Se | Polypeptides having protease activity for use in detergent compositions |
| WO2025202369A1 (en) | 2024-03-27 | 2025-10-02 | Basf Se | Polypeptides having protease activity for use in detergent compositions |
| WO2025202370A1 (en) | 2024-03-27 | 2025-10-02 | Basf Se | Polypeptides having protease activity for use in detergent compositions |
| WO2025213357A1 (en) | 2024-04-09 | 2025-10-16 | The Procter & Gamble Company | Particulate fabric care composition |
| WO2025214720A1 (en) | 2024-04-11 | 2025-10-16 | Unilever Ip Holdings B.V. | Washing machine and washing method |
| WO2025214659A1 (en) | 2024-04-11 | 2025-10-16 | Unilever Ip Holdings B.V. | Washing method |
| WO2025217909A1 (en) | 2024-04-19 | 2025-10-23 | The Procter & Gamble Company | Particulate fabric care product |
| EP4636063A1 (en) | 2024-04-19 | 2025-10-22 | The Procter & Gamble Company | A unit dose laundry detergent product |
| EP4644515A1 (en) | 2024-05-02 | 2025-11-05 | The Procter & Gamble Company | Composition comprising spores and cationic glucan |
| EP4660287A1 (en) | 2024-06-06 | 2025-12-10 | The Procter & Gamble Company | Use of a polysaccharide ester in a laundry detergent composition |
| EP4663733A1 (en) | 2024-06-10 | 2025-12-17 | The Procter & Gamble Company | Use of a graft polymer in a laundering process |
| EP4663732A1 (en) | 2024-06-10 | 2025-12-17 | The Procter & Gamble Company | Use of graft polymer in a laundry detergent composition |
| EP4663737A1 (en) | 2024-06-13 | 2025-12-17 | Unilever IP Holdings B.V. | Laundry unit dose product |
| EP4663738A1 (en) | 2024-06-13 | 2025-12-17 | Unilever IP Holdings B.V. | Laundry unit dose product |
| EP4663728A1 (en) | 2024-06-13 | 2025-12-17 | Unilever IP Holdings B.V. | Method for treating fabrics |
| EP4663729A1 (en) | 2024-06-13 | 2025-12-17 | Unilever IP Holdings B.V. | Method for treating fabrics |
| WO2026012789A1 (en) | 2024-07-08 | 2026-01-15 | Unilever Ip Holdings B.V. | Composition |
| WO2026012788A1 (en) | 2024-07-08 | 2026-01-15 | Unilever Ip Holdings B.V. | Composition |
| WO2026024921A1 (en) | 2024-07-25 | 2026-01-29 | The Procter & Gamble Company | Detergent composition comprising a subtilisin variant and methods of use |
| EP4703460A1 (en) | 2024-08-29 | 2026-03-04 | The Procter & Gamble Company | Water-soluble unit dose article comprising a metalloprotease |
| WO2026050315A1 (en) | 2024-08-29 | 2026-03-05 | Danisco Us Inc. | Subtilisin variants and methods of use |
| EP4703461A1 (en) | 2024-08-29 | 2026-03-04 | The Procter & Gamble Company | Water-soluble unit dose article comprising a metalloprotease |
| CN119662610A (zh) * | 2024-11-20 | 2025-03-21 | 天津科技大学 | 一种碱性蛋白酶突变体及其应用 |
Family Cites Families (94)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE1611703B1 (de) * | 1967-10-11 | 1972-03-09 | Windmoeller & Hoelscher | Vorrichtung zum Abteilen einer bestimmten Stueckzahl innerhalb einer am Ende einer Sack- oder Beutelmaschine gebildeten kontinuierlichen Folge von Saecken oder Beuteln |
| US4028263A (en) * | 1973-08-24 | 1977-06-07 | Colgate-Palmolive Company | Bleaching and brightening detergent composition |
| NL7904044A (nl) * | 1978-05-31 | 1979-12-04 | Unilever Nv | Werkwijze ter bereiding van homogene, vloeibare compo- sities. |
| US4261868A (en) | 1979-08-08 | 1981-04-14 | Lever Brothers Company | Stabilized enzymatic liquid detergent composition containing a polyalkanolamine and a boron compound |
| US4404128A (en) | 1981-05-29 | 1983-09-13 | The Procter & Gamble Company | Enzyme detergent composition |
| GR76237B (pl) | 1981-08-08 | 1984-08-04 | Procter & Gamble | |
| JPS58144105A (ja) | 1982-02-12 | 1983-08-27 | Kurabo Ind Ltd | スケ−ル除去獣毛繊維の製法 |
| GB8310080D0 (en) | 1983-04-14 | 1983-05-18 | Interox Chemicals Ltd | Bleach composition |
| US4760025A (en) | 1984-05-29 | 1988-07-26 | Genencor, Inc. | Modified enzymes and methods for making same |
| US5264366A (en) | 1984-05-29 | 1993-11-23 | Genencor, Inc. | Protease deficient bacillus |
| US5182204A (en) | 1984-05-29 | 1993-01-26 | Genencor International, Inc. | Non-human carbonyl hydrolase mutants, vectors encoding same and hosts transformed with said vectors |
| US5204015A (en) | 1984-05-29 | 1993-04-20 | Genencor International, Inc. | Subtilisin mutants |
| JPS6141481U (ja) * | 1984-08-10 | 1986-03-17 | 三菱電機株式会社 | シ−ム溶接装置 |
| US4797362A (en) * | 1985-06-06 | 1989-01-10 | Lion Corporation | Alkaline proteases and microorganisms producing same |
| US4908773A (en) * | 1987-04-06 | 1990-03-13 | Genex Corporation | Computer designed stabilized proteins and method for producing same |
| US4728455A (en) | 1986-03-07 | 1988-03-01 | Lever Brothers Company | Detergent bleach compositions, bleaching agents and bleach activators |
| DE3750916T2 (de) | 1986-04-30 | 1995-06-01 | Genencor Int | Mutante einer nicht menschlichen Carbonyl-Hydrolase, für diese kodierende DNS-Sequenzen und Vektoren und durch diese Vektoren transformierte Wirte. |
| GB8629837D0 (en) | 1986-12-13 | 1987-01-21 | Interox Chemicals Ltd | Bleach activation |
| US4853871A (en) * | 1987-04-06 | 1989-08-01 | Genex Corporation | Computer-based method for designing stablized proteins |
| US5314692A (en) | 1987-08-24 | 1994-05-24 | Cultor Ltd. | Enzyme premix for feed and method |
| WO1989004552A1 (en) | 1987-10-30 | 1989-05-18 | Lsi Logic Corporation | Method and means of fabricating a semiconductor device package |
| DK6488D0 (da) * | 1988-01-07 | 1988-01-07 | Novo Industri As | Enzymer |
| CN1056187C (zh) * | 1988-02-11 | 2000-09-06 | 金克克国际有限公司 | 新的蛋白水解酶及其在洗涤剂中的应用 |
| US5543302A (en) * | 1988-05-27 | 1996-08-06 | Solvay Enzymes, Inc. | Proteases of altered stability to autolytic degradation |
| DE3841152A1 (de) | 1988-12-07 | 1990-06-13 | Hoechst Ag | Verwendung von bakterienlysierendem enzymprodukt als zusatzstoff zur verbesserung der futterverwertung in der tierproduktion |
| GB8908416D0 (en) | 1989-04-13 | 1989-06-01 | Unilever Plc | Bleach activation |
| JPH03505976A (ja) * | 1989-05-17 | 1991-12-26 | アムジエン・インコーポレーテツド | 多重突然変異スブチリシン |
| EP0405901B1 (en) | 1989-06-26 | 2004-09-01 | Unilever Plc | Enzymatic detergent compositions |
| US5665587A (en) * | 1989-06-26 | 1997-09-09 | Novo Nordisk A/S | Modified subtilisins and detergent compositions containing same |
| DK316989D0 (da) * | 1989-06-26 | 1989-06-26 | Novo Nordisk As | Enzymer |
| GB9003741D0 (en) | 1990-02-19 | 1990-04-18 | Unilever Plc | Bleach activation |
| ES2100925T3 (es) | 1990-05-21 | 1997-07-01 | Unilever Nv | Activacion de blanqueador. |
| EP0563169B2 (en) * | 1990-12-21 | 2006-04-12 | Novozymes A/S | ENZYME MUTANTS HAVING A LOW DEGREE OF VARIATION OF THE MOLECULAR CHARGE OVER A pH RANGE |
| US5769630A (en) * | 1991-02-25 | 1998-06-23 | Louisiana State University, | Subperiosteal bone anchor |
| GB9108136D0 (en) | 1991-04-17 | 1991-06-05 | Unilever Plc | Concentrated detergent powder compositions |
| US5340735A (en) * | 1991-05-29 | 1994-08-23 | Cognis, Inc. | Bacillus lentus alkaline protease variants with increased stability |
| EP0522817A1 (en) | 1991-07-11 | 1993-01-13 | Unilever Plc | Process for preparing manganese complexes |
| DE4224125A1 (de) * | 1991-07-27 | 1993-01-28 | Solvay Enzymes Gmbh & Co Kg | Verfahren zur verbesserung der stabilitaet von enzymen und stabilisierte enzyme |
| GB9118242D0 (en) | 1991-08-23 | 1991-10-09 | Unilever Plc | Machine dishwashing composition |
| GB9124581D0 (en) | 1991-11-20 | 1992-01-08 | Unilever Plc | Bleach catalyst composition,manufacture and use thereof in detergent and/or bleach compositions |
| EP0544490A1 (en) | 1991-11-26 | 1993-06-02 | Unilever Plc | Detergent bleach compositions |
| US5194416A (en) | 1991-11-26 | 1993-03-16 | Lever Brothers Company, Division Of Conopco, Inc. | Manganese catalyst for activating hydrogen peroxide bleaching |
| US5153161A (en) | 1991-11-26 | 1992-10-06 | Lever Brothers Company, Division Of Conopco, Inc. | Synthesis of manganese oxidation catalyst |
| CA2085642A1 (en) | 1991-12-20 | 1993-06-21 | Ronald Hage | Bleach activation |
| GB9127060D0 (en) | 1991-12-20 | 1992-02-19 | Unilever Plc | Bleach activation |
| US5316935A (en) * | 1992-04-06 | 1994-05-31 | California Institute Of Technology | Subtilisin variants suitable for hydrolysis and synthesis in organic media |
| US5454971A (en) * | 1992-05-27 | 1995-10-03 | Showa Denko K.K. | Alkaline lipase, method for producing the same, microorganism producing the same and detergent composition containing alkaline lipase |
| DE4218448A1 (de) | 1992-06-04 | 1993-12-09 | Solvay Enzymes Gmbh & Co Kg | Alkalische Proteasen aus Bacillus pumilus |
| US5256779A (en) | 1992-06-18 | 1993-10-26 | Lever Brothers Company, Division Of Conopco, Inc. | Synthesis of manganese oxidation catalyst |
| US5284944A (en) | 1992-06-30 | 1994-02-08 | Lever Brothers Company, Division Of Conopco, Inc. | Improved synthesis of 1,4,7-triazacyclononane |
| AU682314B2 (en) * | 1992-07-17 | 1997-10-02 | Genencor International, Inc. | High alkaline serine proteases |
| US5280117A (en) | 1992-09-09 | 1994-01-18 | Lever Brothers Company, A Division Of Conopco, Inc. | Process for the preparation of manganese bleach catalyst |
| US5567601A (en) * | 1993-06-01 | 1996-10-22 | University Of Maryland | Subtilisin mutants lacking a primary calcium binding site |
| WO1995005477A1 (en) * | 1993-08-12 | 1995-02-23 | University Of Maryland | Thermostable alkaline metalloprotease produced by a hyphomonas, and preparation thereof |
| US6436690B1 (en) * | 1993-09-15 | 2002-08-20 | The Procter & Gamble Company | BPN′ variants having decreased adsorption and increased hydrolysis wherein one or more loop regions are substituted |
| JP2888986B2 (ja) * | 1993-10-14 | 1999-05-10 | ザ、プロクター、エンド、ギャンブル、カンパニー | プロテアーゼ酵素を含んでなる漂白組成物 |
| HU219851B (hu) * | 1993-10-14 | 2001-08-28 | The Procter And Gamble Company | Proteáz tartalmú tisztítókészítmények |
| MA23346A1 (fr) * | 1993-10-14 | 1995-04-01 | Genencor Int | Variantes de la subtilisine |
| EP0724391B1 (en) * | 1993-10-23 | 1999-05-06 | Imperial Tobacco Limited | Improvements in or relating to smoking articles |
| WO1995023221A1 (en) * | 1994-02-24 | 1995-08-31 | Cognis, Inc. | Improved enzymes and detergents containing them |
| ES2364774T3 (es) * | 1994-02-24 | 2011-09-14 | HENKEL AG & CO. KGAA | Enzimas mejoradas y detergentes que las contienen. |
| US5691295A (en) * | 1995-01-17 | 1997-11-25 | Cognis Gesellschaft Fuer Biotechnologie Mbh | Detergent compositions |
| DK0753057T3 (da) * | 1994-03-29 | 2006-01-30 | Novozymes As | Alkalisk Bacillus-amylase |
| JP3009471B2 (ja) * | 1994-04-07 | 2000-02-14 | ザ、プロクター、エンド、ギャンブル、カンパニー | 金属含有漂白触媒と酸化防止剤とを含む漂白組成物 |
| US6599730B1 (en) * | 1994-05-02 | 2003-07-29 | Procter & Gamble Company | Subtilisin 309 variants having decreased adsorption and increased hydrolysis |
| GB9409336D0 (en) * | 1994-05-10 | 1994-06-29 | Finnfeeds Int Ltd | Use of an enzyme for manufacturing an agent for the treatment and/or prophylaxis of coccidiosis |
| GB9416841D0 (en) | 1994-08-19 | 1994-10-12 | Finnfeeds Int Ltd | An enzyme feed additive and animal feed including it |
| PT796317E (pt) | 1994-12-09 | 2000-08-31 | Procter & Gamble | Composicao para lavagem automatica de loica contendo particulas de peroxidos de diacilo |
| US5534302A (en) | 1995-01-05 | 1996-07-09 | National Science Council | Method of preparing a fiber reinforced modified phenolic resin composite |
| US5534179A (en) | 1995-02-03 | 1996-07-09 | Procter & Gamble | Detergent compositions comprising multiperacid-forming bleach activators |
| AR000862A1 (es) * | 1995-02-03 | 1997-08-06 | Novozymes As | Variantes de una ó-amilasa madre, un metodo para producir la misma, una estructura de adn y un vector de expresion, una celula transformada por dichaestructura de adn y vector, un aditivo para detergente, composicion detergente, una composicion para lavado de ropa y una composicion para la eliminacion del |
| US5837516A (en) * | 1995-03-03 | 1998-11-17 | Genentech, Inc. | Subtilisin variants capable of cleaving substrates containing basic residues |
| US5780285A (en) * | 1995-03-03 | 1998-07-14 | Genentech, Inc. | Subtilisin variants capable of cleaving substrates containing dibasic residues |
| US6455295B1 (en) * | 1995-03-08 | 2002-09-24 | The Procter & Gamble Company | Subtilisin Carlsberg variants having decreased adsorption and increased hydrolysis |
| IL117352A0 (en) * | 1995-03-09 | 1996-07-23 | Procter & Gamble | Thermitase variants having decreased adsorption and increased hydrolysis |
| US6475765B1 (en) * | 1995-03-09 | 2002-11-05 | Procter & Gamble Company | Subtilisin DY variants having decreased adsorption and increased hydrolysis |
| IL117350A0 (en) * | 1995-03-09 | 1996-07-23 | Procter & Gamble | Proteinase k variants having decreased adsorption and increased hydrolysis |
| US5523434A (en) | 1995-03-15 | 1996-06-04 | The Procter & Gamble Company | Synthesis of bleach activators |
| CA2215949A1 (en) * | 1995-04-17 | 1996-10-24 | The Procter & Gamble Company | Preparation and use of composite particles containing diacyl peroxide |
| US5837517A (en) * | 1995-05-05 | 1998-11-17 | Novo Nordisk A/S | Protease variants and compositions |
| TR199701633T1 (xx) | 1995-06-16 | 1998-04-21 | The Procter & Gamble Company | Kobalt kataliz�r i�eren otomatik bula��k makinas� deterjan bile�ikleri. |
| US5597936A (en) | 1995-06-16 | 1997-01-28 | The Procter & Gamble Company | Method for manufacturing cobalt catalysts |
| DE69614114T2 (de) | 1995-06-16 | 2002-04-25 | The Procter & Gamble Company, Cincinnati | Bleichmittelzusammensetzungen, die kobaltkatalysatoren enthalten |
| US5576282A (en) | 1995-09-11 | 1996-11-19 | The Procter & Gamble Company | Color-safe bleach boosters, compositions and laundry methods employing same |
| US5703034A (en) * | 1995-10-30 | 1997-12-30 | The Procter & Gamble Company | Bleach catalyst particles |
| US5762647A (en) * | 1995-11-21 | 1998-06-09 | The Procter & Gamble Company | Method of laundering with a low sudsing granular detergent composition containing optimally selected levels of a foam control agent bleach activator/peroxygen bleaching agent system and enzyme |
| WO1997022680A1 (en) * | 1995-12-20 | 1997-06-26 | The Procter & Gamble Company | Bleach catalyst plus enzyme particles |
| US5883065A (en) * | 1996-01-22 | 1999-03-16 | The Procter & Gamble Company | Phase separated detergent composition |
| WO1997032961A2 (en) * | 1996-03-07 | 1997-09-12 | The Procter & Gamble Company | Detergent compositions comprising improved amylases |
| US6069122A (en) * | 1997-06-16 | 2000-05-30 | The Procter & Gamble Company | Dishwashing detergent compositions containing organic diamines for improved grease cleaning, sudsing, low temperature stability and dissolution |
| US6177392B1 (en) * | 1997-01-13 | 2001-01-23 | Ecolab Inc. | Stable solid block detergent composition |
| US6369011B1 (en) * | 1997-06-04 | 2002-04-09 | The Procter & Gamble Company | Protease enzymes for tough cleaning and/or spot and film reduction and compositions incorporating same |
| US6057171A (en) * | 1997-09-25 | 2000-05-02 | Frequency Technology, Inc. | Methods for determining on-chip interconnect process parameters |
| AR016969A1 (es) * | 1997-10-23 | 2001-08-01 | Procter & Gamble | VARIANTE DE PROTEASA, ADN, VECTOR DE EXPRESIoN, MICROORGANISMO HUESPED, COMPOSICIoN DE LIMPIEZA, ALIMENTO PARA ANIMALES Y COMPOSICIoN PARA TRATAR UN TEXTIL |
-
1998
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- 1998-10-22 MA MA25310A patent/MA25044A1/fr unknown
- 1998-10-22 MA MA25312A patent/MA24811A1/fr unknown
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- 1998-10-23 TR TR2000/01113T patent/TR200001113T2/xx unknown
- 1998-10-23 NZ NZ520771A patent/NZ520771A/en not_active IP Right Cessation
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- 1998-10-23 DE DE69841226T patent/DE69841226D1/de not_active Expired - Lifetime
- 1998-10-23 WO PCT/US1998/022500 patent/WO1999020769A2/en not_active Ceased
- 1998-10-23 CA CA2308113A patent/CA2308113C/en not_active Expired - Fee Related
- 1998-10-23 DK DK98955111T patent/DK1025240T3/da active
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- 1998-10-23 EP EP98953936A patent/EP1025239B1/en not_active Expired - Lifetime
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- 1998-10-23 AU AU11181/99A patent/AU758371B2/en not_active Ceased
- 1998-10-23 CA CA002672500A patent/CA2672500A1/en not_active Abandoned
- 1998-10-23 ES ES98955079T patent/ES2319470T5/es not_active Expired - Lifetime
- 1998-10-23 DK DK05006461.7T patent/DK1571199T3/da active
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- 1998-10-23 CN CNB988126370A patent/CN1198912C/zh not_active Expired - Lifetime
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- 1998-10-23 CN CNB2004100399770A patent/CN1295313C/zh not_active Expired - Lifetime
- 1998-10-23 DE DE69839057T patent/DE69839057T2/de not_active Expired - Lifetime
- 1998-10-23 CN CNB988126109A patent/CN1191347C/zh not_active Expired - Lifetime
- 1998-10-23 DE DE69839677T patent/DE69839677D1/de not_active Expired - Lifetime
- 1998-10-23 HU HU0100859A patent/HUP0100859A2/hu unknown
- 1998-10-23 CA CA2307638A patent/CA2307638C/en not_active Expired - Fee Related
- 1998-10-23 EP EP98955079A patent/EP1025194B2/en not_active Expired - Lifetime
- 1998-10-23 CZ CZ20001493A patent/CZ300347B6/cs not_active IP Right Cessation
- 1998-10-23 AT AT98955111T patent/ATE314478T1/de active
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1999
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2000
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2001
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2002
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2006
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