ES2131797T3 - Procedimiento para la purificacion de polipeptidos. - Google Patents

Procedimiento para la purificacion de polipeptidos.

Info

Publication number
ES2131797T3
ES2131797T3 ES95903202T ES95903202T ES2131797T3 ES 2131797 T3 ES2131797 T3 ES 2131797T3 ES 95903202 T ES95903202 T ES 95903202T ES 95903202 T ES95903202 T ES 95903202T ES 2131797 T3 ES2131797 T3 ES 2131797T3
Authority
ES
Spain
Prior art keywords
silica particles
polypeptide
mixture
polypeptides
purification
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES95903202T
Other languages
English (en)
Inventor
Stuart E Builder
Charles V Olson
David Reifsnyder
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Genentech Inc
Original Assignee
Genentech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genentech Inc filed Critical Genentech Inc
Application granted granted Critical
Publication of ES2131797T3 publication Critical patent/ES2131797T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/521—Chemokines
    • C07K14/523—Beta-chemokines, e.g. RANTES, I-309/TCA-3, MIP-1alpha, MIP-1beta/ACT-2/LD78/SCIF, MCP-1/MCAF, MCP-2, MCP-3, LDCF-1, LDCF-2
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4743—Insulin-like growth factor binding protein
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/65—Insulin-like growth factors, i.e. somatomedins, e.g. IGF-1, IGF-2

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Diabetes (AREA)
  • Endocrinology (AREA)
  • Analytical Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

SE SUMINISTRA UN PROCESO PARA SEPARAR SELECTIVAMENTE UN POLIPEPTIDO DE INTERES DE COMPONENTES DE HIDROFOBICIDAD DIFERENTE EN UNA MEZCLA QUE COMPRENDE LOS PASOS DE: A) HACER PASAR LA MEZCLA A TRAVES DE PARTICULAS DE SILICE NO DERIVATIZADAS DE FORMA QUE EL POLIPEPTIDO SE ADHIERA A LAS PARTICULAS DE SILICE; B) LAVAR LAS PARTICULAS DE SILICE PARA ELIMINAR LAS IMPUREZAS; C) LEVIGAR EL POLIPEPTIDO DE LAS PARTICULAS DE SILICE CON UN AGENTE NEUTRALIZADOR QUE COMPRENDA UN SOLVENTE APROTICO POLAR O ALCOHOLICO Y UNA TIERRA ALCALINA, UN METAL ALCALINO O UNA SAL DE AMONIO INORGANICA.
ES95903202T 1993-12-13 1994-12-06 Procedimiento para la purificacion de polipeptidos. Expired - Lifetime ES2131797T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/166,337 US5451660A (en) 1993-12-13 1993-12-13 Method for purifying polypeptides

Publications (1)

Publication Number Publication Date
ES2131797T3 true ES2131797T3 (es) 1999-08-01

Family

ID=22602848

Family Applications (1)

Application Number Title Priority Date Filing Date
ES95903202T Expired - Lifetime ES2131797T3 (es) 1993-12-13 1994-12-06 Procedimiento para la purificacion de polipeptidos.

Country Status (9)

Country Link
US (1) US5451660A (es)
EP (1) EP0734393B1 (es)
JP (1) JP3843373B2 (es)
AT (1) ATE178612T1 (es)
CA (1) CA2177575C (es)
DE (1) DE69417765T2 (es)
DK (1) DK0734393T3 (es)
ES (1) ES2131797T3 (es)
WO (1) WO1995016701A1 (es)

Families Citing this family (37)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0779927A1 (en) * 1994-09-08 1997-06-25 Chiron Corporation A method of improved production of insulin-like growth factor
US6756484B1 (en) 1995-04-14 2004-06-29 Cephalon, Inc. IGF-I purification process
US5650496A (en) * 1995-04-14 1997-07-22 Cephalon, Inc. IGF-I purification process
AU6167596A (en) * 1995-06-07 1996-12-30 Chiron Corporation Methods for purifying authentic igf from yeast hosts
US7071313B1 (en) 1995-06-07 2006-07-04 Cephalon, Inc. Methods of purifying authentic IGF from yeast hosts
US7193042B1 (en) 1995-06-07 2007-03-20 Chiron Corporation Methods for purifying authentic IGF from yeast hosts
US20040086494A1 (en) * 1996-10-07 2004-05-06 John Constance Mary Immune privileged cells for delivery of proteins and peptides
AU729459B2 (en) * 1996-11-15 2001-02-01 Genentech Inc. Purification of neurotrophins
DE69738075T2 (de) * 1996-11-27 2008-05-21 Genentech, Inc., South San Francisco Affinitätsreinigung von polypeptiden an einer protein a-matrix
TW541179B (en) * 1997-03-19 2003-07-11 Green Cross Corp Process for preparing immunoglobulin preparation
US6265542B1 (en) 1997-10-24 2001-07-24 Genentech, Inc. Purification of molecules
US6121421A (en) * 1998-08-21 2000-09-19 Abbott Laboratories Methods for isolating recombinant β-casein
US7399751B2 (en) * 1999-11-04 2008-07-15 Sertoli Technologies, Inc. Production of a biological factor and creation of an immunologically privileged environment using genetically altered Sertoli cells
KR100356140B1 (ko) * 1999-07-08 2002-10-19 한미약품공업 주식회사 인간 과립구 콜로니 자극인자 변이체 및 이의 생산 방법
EP1401808B1 (en) 2001-05-24 2009-07-08 Neuren Pharmaceuticals Limited Gpe analogs and peptidomimetics
US7714020B2 (en) * 2001-05-24 2010-05-11 Neuren Pharmaceuticals Limited Treatment of non-convulsive seizures in brain injury using G-2-methyl-prolyl glutamate
US20070004641A1 (en) * 2001-05-24 2007-01-04 Neuren Pharmaceuticals Limited Cognitive enhancement and cognitive therapy using glycyl-L-2-methylprolyl-L-glutamate
US7605177B2 (en) * 2001-05-24 2009-10-20 Neuren Pharmaceuticals Limited Effects of glycyl-2 methyl prolyl glutamate on neurodegeneration
KR100436655B1 (ko) * 2001-07-25 2004-06-22 (주)엘피스바이오텍 광범위한 단백질에 적용할 수 있는 단백질의 농축 및정제 방법
US7157276B2 (en) * 2003-06-20 2007-01-02 Biogen Idec Inc. Use of depth filtration in series with continuous centrifugation to clarify mammalian cell cultures
CA2531022C (en) * 2003-07-03 2014-09-09 Jannette Dufour Compositions containing sertoli cells and myoid cells and use thereof in cellular transplants
DK1969007T3 (da) * 2005-12-20 2013-11-25 Bristol Myers Squibb Co Sammensætninger og fremgangsmåder til fremstilling af en sammensætning
AR058568A1 (es) 2005-12-20 2008-02-13 Bristol Myers Squibb Co Metodos para producir una composicion con moleculas ctla4-ig a partir de un medio de cultivo
WO2008106043A2 (en) * 2007-02-26 2008-09-04 Alltech Associates Inc. Ultra-fast chromatography
ES2657055T3 (es) * 2007-08-09 2018-03-01 Wyeth Llc Uso de perfusión para mejorar la producción de un cultivo de células alimentado por lotes en biorreactores
JP5397800B2 (ja) * 2008-07-31 2014-01-22 国立大学法人広島大学 タンパク質の精製方法
US8846878B1 (en) * 2009-01-23 2014-09-30 Cubrc Corporation Method and device for isolating a protein sample
KR101382404B1 (ko) * 2012-01-04 2014-04-14 대한민국 봉독의 대량 정제방법
KR101504824B1 (ko) 2013-05-31 2015-03-23 씨제이헬스케어 주식회사 고당화된 지속형 인간 성장호르몬 단백질 및 이의 제조방법
US9902753B2 (en) 2012-06-05 2018-02-27 Cj Healthcare Corporation Method of purifying a long-acting human growth hormone
KR101460266B1 (ko) 2012-06-05 2014-11-11 씨제이헬스케어 주식회사 신규한 지속형 인간 성장호르몬의 정제 방법
JP6110611B2 (ja) * 2012-07-18 2017-04-05 株式会社東芝 アミンの回収方法および分析方法
EP2812091B1 (en) 2012-09-17 2021-03-10 W.R. Grace & CO. - CONN. Chromatography media and devices
EP2811983B1 (en) 2012-09-17 2019-05-01 W.R. Grace & CO. - CONN. Functionalized particulate support material and methods of making and using the same
ES2887110T3 (es) 2014-01-16 2021-12-21 Grace W R & Co Medios para cromatografía de afinidad y dispositivos para cromatografía
PL3137209T3 (pl) 2014-05-02 2023-01-02 W.R. Grace & Co. - Conn. Funkcjonalizowany materiał nośnikowy i sposoby wytwarzania oraz stosowania funkcjonalizowanego materiału nośnikowego
KR102566292B1 (ko) 2015-06-05 2023-08-10 더블유.알. 그레이스 앤드 캄파니-콘. 흡착성 바이오프로세싱 정화제와 이의 제조 및 사용 방법

Family Cites Families (45)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE295872C (es) *
CH187815A (fr) * 1936-05-01 1936-11-30 Henri Dr Paschoud Appareil d'éclairage d'un champ opératoire.
CH243336A (de) * 1945-02-12 1946-07-15 Ag Sandoz Verfahren zur Herstellung eines metallisierbaren Disazofarbstoffes.
US3904751A (en) * 1970-12-22 1975-09-09 Behringwerke Ag Process for isolating a fibrin stabilizing factor from human placenta
JPS5620287B2 (es) * 1972-06-19 1981-05-13
US3869482A (en) * 1972-07-31 1975-03-04 Etapharm Chem Pharm Lab G M B Method of producing highly purified phosphatides
JPS5224594B2 (es) * 1974-02-25 1977-07-01
JPS51118810A (en) * 1975-04-10 1976-10-19 Fujiwa Kako Kk A process for preparing proteolytic enzyme inhibitor from human urine
SU560614A1 (ru) * 1976-02-11 1977-06-05 Среднеазиатский Научно-Исследовательский И Проектно-Конструкторский Институт Пищевой Промышленности Способ получени фосфолипазы д
FR2422720A2 (fr) * 1977-04-26 1979-11-09 Elf Aquitaine Separation et purification de proteines par chromatographie
DE3147842A1 (de) * 1981-12-03 1983-06-23 Hoechst Ag, 6230 Frankfurt "verfahren zur trennung von gemischen aus insulin, insulinderivaten und gegebenenfalls verunreinigungen"
DD209187A1 (de) * 1982-09-08 1984-04-25 Adw Ddr Verfahren zur gewinnung von ergosterol und ubichinonen
JPS59159753A (ja) * 1983-03-02 1984-09-10 T Hasegawa Co Ltd 風味改良剤の製法
DD217823A1 (de) * 1983-07-28 1985-01-23 Adw Ddr Verfahren zur gewinnung von erythrogenem toxin typ c
JPS60197629A (ja) * 1984-03-16 1985-10-07 Chemo Sero Therapeut Res Inst ＨＢｓ抗原の精製方法
US5028531A (en) * 1984-03-19 1991-07-02 Fujisawa Pharmaceutical Company, Ltd. IGF-I fusion proteins; protection of IGF-I from degradation by host cell; and processes for the production thereof
US4652529A (en) * 1984-10-02 1987-03-24 Syntex (U.S.A.) Inc. Serum pretreatment for tricyclic antidepressant drug assays
DD280174A1 (de) * 1985-04-24 1990-06-27 Akad Wissenschaften Ddr Verfahren zur beseitigung von immunglobulinaggregaten in immunglobulinpraeparaten
SU1272227A1 (ru) * 1985-10-04 1986-11-23 Ордена Трудового Красного Знамени Институт Высокомолекулярных Соединений Ан Ссср Способ эксклюзионной жидкостной хроматографии полимеров
FR2598917B1 (fr) * 1986-05-21 1988-08-19 Pasteur Institut Nouvelle lymphokine suppressive de l'activation plaquettaire, son procede d'isolement et de purification et medicaments la contenant
JPH0789951B2 (ja) * 1986-06-18 1995-10-04 財団法人阪大微生物病研究会 遺伝子発現産物の精製法
JPS63225373A (ja) * 1986-07-24 1988-09-20 Sumitomo Pharmaceut Co Ltd 新規チアゾリジン−４−オン誘導体およびその酸付加塩
US4725673A (en) * 1986-08-29 1988-02-16 Alpha Therapeutic Corporation Plasma fraction purification using silica resin bound to a ligand
US4777242A (en) * 1986-10-10 1988-10-11 Phillips Petroleum Company Purification of recombinant tumor necrosis factor
DE3639949A1 (de) * 1986-11-22 1988-06-09 Diagen Inst Molekularbio Verfahren zur trennung von langkettigen nukleinsaeuren
US4732683A (en) * 1986-12-02 1988-03-22 Biospectrum, Inc. Purification method for alpha interferon
US4894330A (en) * 1986-12-23 1990-01-16 Cetus Corporation Purification of recombinant beta-interferon incorporating RP-HPLC
US4908432A (en) * 1987-01-09 1990-03-13 New York University Novel polypeptide having gamma-interferon activity
US5084384A (en) * 1987-04-23 1992-01-28 Monsanto Company Secretion of insulin-like growth factor-I
JPS6419024A (en) * 1987-07-15 1989-01-23 Tosoh Corp Separation of immunoglobulin g subclass
JPS6451097A (en) * 1987-08-21 1989-02-27 Toray Industries Purification of fused protein
US5004688A (en) * 1988-04-15 1991-04-02 Phillips Petroleum Company Purification of hepatitis proteins
DD286721A7 (de) * 1988-05-30 1991-02-07 Adw Ddr Verfahren zur gewinnung von protein a
DD298275A5 (de) * 1988-05-30 1992-02-13 Zentralinstitut Fuer Mikrobiologie U. Experiment. Therapie D. Adw D. Ddr (Zimet),De Verfahren zur reindarstellung von protein a
KR900000748A (ko) * 1988-06-17 1990-01-31 안시환 무인반송차의 교통 통제용 센서 유니트
JP2517100B2 (ja) * 1989-03-08 1996-07-24 協和醗酵工業株式会社 ヒト顆粒球コロニ―刺激因子活性を有する蛋白質の精製法
US5158875A (en) * 1989-08-25 1992-10-27 Amgen Inc. Production of biologically active insulin-like growth factor i from high expression host cell systems
FR2653034A1 (fr) * 1989-10-13 1991-04-19 Centre Nat Rech Scient Procede de preparation d'un produit particulaire poreux regiospecifique, produits obtenus et application notamment aux separations chromatographiques.
DE69013471T2 (de) * 1989-12-05 1995-03-30 Merck & Co Inc Methode zur Stabilisierung von rekombinanten Hepatitis-B-Virus-Oberflächenproteinen aus Hefe.
JPH04130131A (ja) * 1990-09-20 1992-05-01 Polyplastics Co 加工が容易な全芳香族ポリエステル
KR920008377A (ko) * 1990-10-16 1992-05-27 까롤 미셀 벵그리예프 차량현가용 진동방지절연부재의 제조방법
DE4042745B4 (de) * 1990-10-24 2009-12-03 Skf Gmbh Federnd einstellbare Spannvorrichtung
US5231178A (en) * 1991-01-16 1993-07-27 The Salk Institute Biotechnology/Industrial Associates, Inc. Method for the purification of intact, correctly-folded insulin-like growth factor-1
US5449750A (en) * 1991-06-20 1995-09-12 Snow Brand Milk Products Co., Ltd. Prolyl endopeptidase inhibitors SNA-115 and SNA-115T, and process for the production and productive strain thereof
US5278284A (en) * 1992-05-14 1994-01-11 Miller Brewing Company Protein purification method

Also Published As

Publication number Publication date
DK0734393T3 (da) 2000-07-10
WO1995016701A1 (en) 1995-06-22
CA2177575C (en) 2008-04-22
DE69417765T2 (de) 1999-09-02
ATE178612T1 (de) 1999-04-15
EP0734393B1 (en) 1999-04-07
JPH09506617A (ja) 1997-06-30
DE69417765D1 (de) 1999-05-12
US5451660A (en) 1995-09-19
CA2177575A1 (en) 1995-06-22
EP0734393A1 (en) 1996-10-02
JP3843373B2 (ja) 2006-11-08

Similar Documents

Publication Publication Date Title
ES2131797T3 (es) Procedimiento para la purificacion de polipeptidos.
PT1308455E (pt) Composicao compreendendo anticorpos anti-her2
EP1308456A3 (en) Antibody purification by ion exchange chromatography
AR010073A1 (es) Purificacion de proteinas.
CA2183888A1 (en) Antibody purification
BR8302349A (pt) Sistema e processo para distribuir gas comprimido
ATE212723T1 (de) Analysengerät mit einer vorrichtung zum abtrennen magnetischer mikropartikel
GB2151995B (en) Apparatus for the suspension of an aircraft engine cowling
NZ232833A (en) Intercellular adhesion molecule-2 (icam-2) and functional derivatives thereof
DK583284D0 (da) Fremgangsmaade og apparat til fordeling af vandrensende kemikalier
ATE34177T1 (de) Verfahren zur gewinnung einer peptidbindungen enthaltenden verbindung.
CA2133436A1 (en) Methods for producing eg iii cellulase, xylanase or a mixture thereof using polyethylene glycol
DK0701623T3 (da) Fremgangsmåde til fremstilling af N-acetyl-neuraminsyre
ES518315A0 (es) Procedimiento semicontinuo para la preparacion de silicio puro.
AU2813392A (en) Process for recovering sulfuric acid from metallic sulfate-containing exhaust sulfuric acid
SE0102864D0 (sv) Antimicrorobial agent
GB9324729D0 (en) Process for the analysis of gaseous components by mass spectrometry
ZA821440B (en) Process of producing an so3-containing gas for use in the purification of an exhaust gas in an electrostatic precipitator
WO1999061475A8 (de) Verfahren zur trennung und/oder isolierung von plasmaproteinen mit annularer chromatographie
JPS57120710A (en) Fixing of mounting
SE9202012D0 (sv) Granat
JPS5392800A (en) Tachypleus tridentatus lectin and process for its purification
PL237079A1 (en) Method of separation and determination of di-2-ethylhexyl/-peroxodicarbonate and its contaminants by means of high performance liquid chromatography
Carr Move to Save Thyssen--Krupp Link-Up
陈贵云 Finite groups with Schmidt group as automorphism group

Legal Events

Date Code Title Description
FG2A Definitive protection

Ref document number: 734393

Country of ref document: ES