ES2131797T3 - Procedimiento para la purificacion de polipeptidos. - Google Patents

Procedimiento para la purificacion de polipeptidos.

Info

Publication number
ES2131797T3
ES2131797T3 ES95903202T ES95903202T ES2131797T3 ES 2131797 T3 ES2131797 T3 ES 2131797T3 ES 95903202 T ES95903202 T ES 95903202T ES 95903202 T ES95903202 T ES 95903202T ES 2131797 T3 ES2131797 T3 ES 2131797T3
Authority
ES
Spain
Prior art keywords
silica particles
polypeptide
mixture
polypeptides
purification
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES95903202T
Other languages
English (en)
Inventor
Stuart E Builder
Charles V Olson
David Reifsnyder
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Genentech Inc
Original Assignee
Genentech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genentech Inc filed Critical Genentech Inc
Application granted granted Critical
Publication of ES2131797T3 publication Critical patent/ES2131797T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/521—Chemokines
    • C07K14/523—Beta-chemokines, e.g. RANTES, I-309/TCA-3, MIP-1alpha, MIP-1beta/ACT-2/LD78/SCIF, MCP-1/MCAF, MCP-2, MCP-3, LDCF-1, LDCF-2
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4743—Insulin-like growth factor binding protein
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/65—Insulin-like growth factors, i.e. somatomedins, e.g. IGF-1, IGF-2

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Diabetes (AREA)
  • Analytical Chemistry (AREA)
  • Endocrinology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

SE SUMINISTRA UN PROCESO PARA SEPARAR SELECTIVAMENTE UN POLIPEPTIDO DE INTERES DE COMPONENTES DE HIDROFOBICIDAD DIFERENTE EN UNA MEZCLA QUE COMPRENDE LOS PASOS DE: A) HACER PASAR LA MEZCLA A TRAVES DE PARTICULAS DE SILICE NO DERIVATIZADAS DE FORMA QUE EL POLIPEPTIDO SE ADHIERA A LAS PARTICULAS DE SILICE; B) LAVAR LAS PARTICULAS DE SILICE PARA ELIMINAR LAS IMPUREZAS; C) LEVIGAR EL POLIPEPTIDO DE LAS PARTICULAS DE SILICE CON UN AGENTE NEUTRALIZADOR QUE COMPRENDA UN SOLVENTE APROTICO POLAR O ALCOHOLICO Y UNA TIERRA ALCALINA, UN METAL ALCALINO O UNA SAL DE AMONIO INORGANICA.
ES95903202T 1993-12-13 1994-12-06 Procedimiento para la purificacion de polipeptidos. Expired - Lifetime ES2131797T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/166,337 US5451660A (en) 1993-12-13 1993-12-13 Method for purifying polypeptides

Publications (1)

Publication Number Publication Date
ES2131797T3 true ES2131797T3 (es) 1999-08-01

Family

ID=22602848

Family Applications (1)

Application Number Title Priority Date Filing Date
ES95903202T Expired - Lifetime ES2131797T3 (es) 1993-12-13 1994-12-06 Procedimiento para la purificacion de polipeptidos.

Country Status (9)

Country Link
US (1) US5451660A (es)
EP (1) EP0734393B1 (es)
JP (1) JP3843373B2 (es)
AT (1) ATE178612T1 (es)
CA (1) CA2177575C (es)
DE (1) DE69417765T2 (es)
DK (1) DK0734393T3 (es)
ES (1) ES2131797T3 (es)
WO (1) WO1995016701A1 (es)

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KR101382404B1 (ko) * 2012-01-04 2014-04-14 대한민국 봉독의 대량 정제방법
JP6231559B2 (ja) * 2012-06-05 2017-11-15 シージェイ ヘルスケア コーポレイションＣｊ Ｈｅａｌｔｈｃａｒｅ Ｃｏｒｐｏｒａｔｉｏｎ 高グリコシル化された持続型ヒト成長ホルモンタンパク質の製造方法及び精製方法
KR101504824B1 (ko) 2013-05-31 2015-03-23 씨제이헬스케어 주식회사 고당화된 지속형 인간 성장호르몬 단백질 및 이의 제조방법
KR101460266B1 (ko) 2012-06-05 2014-11-11 씨제이헬스케어 주식회사 신규한 지속형 인간 성장호르몬의 정제 방법
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MY195756A (en) 2012-09-17 2023-02-09 Grace W R & Co Functionalized Particulate Support Material and Methods of Making and Using the Same
EP3094390B1 (en) 2014-01-16 2021-07-07 W.R. Grace & CO. - CONN. Affinity chromatography media and chromatography devices
EP3137209B1 (en) 2014-05-02 2022-10-05 W.R. Grace & CO. - CONN. Functionalized support material and methods of making and using functionalized support material
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Also Published As

Publication number Publication date
DK0734393T3 (da) 2000-07-10
JP3843373B2 (ja) 2006-11-08
EP0734393B1 (en) 1999-04-07
ATE178612T1 (de) 1999-04-15
JPH09506617A (ja) 1997-06-30
DE69417765T2 (de) 1999-09-02
WO1995016701A1 (en) 1995-06-22
EP0734393A1 (en) 1996-10-02
CA2177575C (en) 2008-04-22
DE69417765D1 (de) 1999-05-12
US5451660A (en) 1995-09-19
CA2177575A1 (en) 1995-06-22

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