HK75493A - Procaryotic carbonyl hydrolases, methods, dna, vectors and transformed hosts for producing them, and detergent compositions containing them - Google Patents

Procaryotic carbonyl hydrolases, methods, dna, vectors and transformed hosts for producing them, and detergent compositions containing them

Info

Publication number
HK75493A
HK75493A HK754/93A HK75493A HK75493A HK 75493 A HK75493 A HK 75493A HK 754/93 A HK754/93 A HK 754/93A HK 75493 A HK75493 A HK 75493A HK 75493 A HK75493 A HK 75493A
Authority
HK
Hong Kong
Prior art keywords
enzyme
mutation
subtilisin
dna
mutant
Prior art date
Application number
HK754/93A
Other languages
English (en)
French (fr)
Inventor
Richard Ray Bott
Eugenio Ferrari
James Allen Wells
David Aaron Estell
Dennis James Henner
Original Assignee
Genencor International Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=27560079&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=HK75493(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority claimed from US06/614,612 external-priority patent/US4760025A/en
Application filed by Genencor International Inc filed Critical Genencor International Inc
Publication of HK75493A publication Critical patent/HK75493A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07—Bacillus

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Detergent Compositions (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Claims (22)

1. Verfahren, das die Bewirkung einer Mutation in einem Bacillus Subtilisinenzym oder seinem Prä-oder Präproenzym in einer oder mehreren der Positionen, die Tyr-1, Asn+155, Tyr+104, Met+222, Gly+166, Gly+169, Glu+156, Ser+33, Phe+189, Tyr+217 und Ala+152 in B. amyloliquefaciens Subtilisin oder seinem Prä- oder Präproenzym entsprechen, und Prüfen in bezug auf eine gewünschte Aktivitätsveränderung in dem Enzym umfaßt, die sich aus der genannten Mutation ergibt.
2. Verfahren, das die Herstellung eines mutanten Subtilisinenzyms, das die gewünschte Aktivitätsveränderung aufweist, nach seiner Identifizierung gemäß dem Verfahren nach Anspruch 1 umfaßt.
3. Verfahren nach Anspruch 1 oder 2, worin die Mutation die Substitution einer Aminosäure in einer Position umfaßt, die +169 von B. amyloliquefaciens Subtilisin entspricht, wodurch ein Enzym von veränderter spezifischer Aktivität erzeugt wird.
4. Verfahren nach Anspruch 1 oder 2, worin die Mutation die Substitution einer Aminosäure in einer Position umfaßt, die +156 von B. amyloliquefaciens entspricht und ein Enzym von veränderter pH-Aktivität erzeugt.
5. Verfahren nach Anspruch 1 oder 2, worin die Mutation die Substitution einer Aminosäure in einer Position umfaßt, die +222 von B. amyloliquefaciens entspricht und ein Enzym von verstärkter Oxydationsstabilität und/oder veränderter pH-Aktivität erzeugt.
6. Verfahren nach Anspruch 5, worin die Mutation die Substitution von Ala oder Ser für den Rest in Position +222 umfaßt.
7. Verfahren nach Anspruch 1 oder 2, worin die Mutation die Substitution einer Aminosäure in einer Stellung umfaßt, die +166 in B. amyloliquefaciens entspricht und ein Enzym von verstärkter spezifischer Aktivität und/oder veränderter Substratspezifität erzeugt.
8. Verwendung eines mutanten Enzyms nach einem der Ansprüche 1 bis 7 in einer Waschmittelzusammensetzung.
9. Verwendung nach Anspruch 8, worin das Waschmittel ein lineares Alkylbenzolsulfonat, Alkyl- äthoxyliertes-Sulfat, sulfatierter linearer Alkohol oder äthoxylierter linearer Alkohol ist.
10. Verfahren umfassend
Schaffen einer DNA Sequenz, die ein Bacillus Subtilisinenzym kodiert, in dem eine Mutation in einer oder mehreren der Positionen durchgeführt wurde, die Tyr-1, Asn+155, Tyr+104, Met+222, Gly+166, Gly+169, Glu+156, Ser+33, Phe+189, Tyr+217 und Ala+152 in B. amyloliquefaciens Subtilisin oder seinem Prä- oder Präproenzym entsprechen,
Transformiern einer Wirtszelle mit der DNA, sodaß die Kodierungssequenz darin exprimiert wird, und
Prüfen in bezug auf eine gewünschte Aktivitätsveränderung in dem Enzym, das sich aus der genannten Mutation ergibt.
11. Verfahren umfassend nach der Identifizierung eines gewünschten mutanten Enzyms nach dem Verfahren nach Anspruch 10, die Expression einer DNA-Sequenz, die das genannte Enzym kodiert, in einer operabel damit transformierten, rekombinanten Wirtszelle, um ein mutantes Subtilisin, Pro- oder Präprosubtilisin zu erhalten.
12. Verfahren nach Anspruch 10 oder 11, worin die Mutation als die Substitution eines oder mehrerer Methionin-, Tryptophan, Cystein- oder Lysinreste durch einen substituenten Aminosäurerest, der nicht Methionin, Tryptophan, Cystein oder Lysin ist, vorzugsweise durch Alanin oder Serin exprimiert wird.
13. Verfahren nach einem der Ansprüche 10, 11 und 12, worin die Mutation als Mutant exprimiert wird, der eine Veränderung in einer oder mehreren der Eigenschaften Oxydationsstabilität, Km, Kcat, Kcat/Km-Verhältnis, Substratspezifität, spezifische Aktivität oder pH-Optimum des Enzyms vor der Mutation aufweist.
14. Verfahren nach Anspruch 13, worin die Mutation die Oxydationsstabilität des Enzyms verändert.
15. Verfahren nach Anspruch 13, worin ein Mutant gewonnen wird, der eine verschiedene Substratspezifität aufweist.
16. Verfahren nach einem der Ansprüche 10 bis 15, worin die Mutation die Substitution einer Aminosäure in einer Position umfaßt, die +169 von B. amyloliquefaciens Subtilisin entspricht und ein Enzym von veränderter spezifischer Aktivität erzeugt.
17. Verfahren nach einem der Ansprüche 10 bis 16, worin die Mutation die Substitution einer Aminosäure in einer Position umfaßt, die +156 von B. amyloliquefaciens entspricht und ein Enzym von verändertem pH-Stabilitätsbereich erzeugt.
18. Verfahren nach einem der Ansprüche 10 bis 17, weiters umfassend das Einbringen des mutanten Enzyms in eine Waschmittelzusammensetzung.
19. Verfahren nach Anspruch 18, worin das Waschmittel ein lineares Alkylbenzolsulfonat, Alkyl- äthoxyliertes-Sulfat, sulfatierter linearer Alkohol oder äthoxylierter linearer Alkohol ist.
20. Verfahren nach einem der Ansprüche 10 bis 19, worin ein Subtilisin in Form eines Prosubtilisins oder Präprosubtilisins erzeugt wird.
21. Verfahren nach einem der Ansprüche 10 bis 20, worin die rekombinante Wirtszelle ein Stamm von Bacillus, vorzugsweise ein Bacillus subtilis ist.
22. Verfahren nach einem der Ansprüche 10 bis 21, worin die Wirtszelle mit einer DNA-Sequenz transformiert wurde, die das Enzym operabel an seine Signalsequenz gebunden kodiert.
HK754/93A 1983-06-24 1993-07-29 Procaryotic carbonyl hydrolases, methods, dna, vectors and transformed hosts for producing them, and detergent compositions containing them HK75493A (en)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US50741983A 1983-06-24 1983-06-24
US61461584A 1984-05-29 1984-05-29
US61461684A 1984-05-29 1984-05-29
US61461784A 1984-05-29 1984-05-29
US61449184A 1984-05-29 1984-05-29
US06/614,612 US4760025A (en) 1984-05-29 1984-05-29 Modified enzymes and methods for making same

Publications (1)

Publication Number Publication Date
HK75493A true HK75493A (en) 1993-08-06

Family

ID=27560079

Family Applications (3)

Application Number Title Priority Date Filing Date
HK754/93A HK75493A (en) 1983-06-24 1993-07-29 Procaryotic carbonyl hydrolases, methods, dna, vectors and transformed hosts for producing them, and detergent compositions containing them
HK753/93A HK75393A (en) 1983-06-24 1993-07-29 Dna mutagenesis method
HK992/93A HK99293A (en) 1983-06-24 1993-09-23 Bacillus incapable of excreting subtilisin or neutral protease

Family Applications After (2)

Application Number Title Priority Date Filing Date
HK753/93A HK75393A (en) 1983-06-24 1993-07-29 Dna mutagenesis method
HK992/93A HK99293A (en) 1983-06-24 1993-09-23 Bacillus incapable of excreting subtilisin or neutral protease

Country Status (8)

Country Link
EP (4) EP0246678B1 (de)
JP (3) JP2594533B2 (de)
AT (2) ATE60356T1 (de)
DE (3) DE3484019D1 (de)
DK (3) DK175278B1 (de)
HK (3) HK75493A (de)
IE (1) IE81141B1 (de)
NZ (1) NZ208612A (de)

Families Citing this family (224)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IE81141B1 (en) 1983-06-24 2000-04-05 Genencor Int Procaryotic carbonyl hydrolases
CA1311429C (en) * 1983-07-06 1992-12-15 Vasantha Nagarajan Vector for polypeptides in bacilli
JPH0659224B2 (ja) * 1983-09-12 1994-08-10 工業技術院長 Ｄνａ塩基配列及びそのｄνａ塩基配列を含む組換え体プラスミドの創製法
JPH07106156B2 (ja) * 1983-10-28 1995-11-15 ジェネティックス、インスティチュ−ト ファクタ−▲ｖｉｉｉ▼および関連生産物の製造
EP0462632A1 (de) * 1983-10-28 1991-12-27 Genetics Institute, Inc. Verfahren zur Herstellung von gewünschten menschlichen Proteinen durch Rekombinant-DNA-Techniken
DE3484346D1 (de) * 1983-12-28 1991-05-02 Agency Ind Science Techn Dns-basensequenz enthaltende regionen einbegriffen in der herstellung und sekretion eines proteins, rekombinante dns das ganze oder einen teil der dns-basensequenz enthaltend und verfahren zur herstellung von proteinen unter verwendung der rekombinanten dns.
US5411873A (en) * 1984-05-29 1995-05-02 Genencor, Inc. Process for producing heterologous polypeptides
US5185258A (en) 1984-05-29 1993-02-09 Genencor International, Inc. Subtilisin mutants
US5972682A (en) * 1984-05-29 1999-10-26 Genencor International, Inc. Enzymatically active modified subtilisins
US4801537A (en) * 1984-06-08 1989-01-31 Genex Corporation Vector for expression of polypeptides in bacilli
US5840522A (en) * 1984-09-20 1998-11-24 Chiron Corporation Recombinant lectins
US4828994A (en) * 1984-09-21 1989-05-09 Genex Corporation Bacillus strains with reduced extracellular protease levels
US4752585A (en) * 1985-12-17 1988-06-21 Cetus Corporation Oxidation-resistant muteins
CA1340265C (en) * 1985-01-18 1998-12-15 Kirston E. Koths Oxidation resistant muteins
US6084073A (en) * 1985-03-25 2000-07-04 Chiron Corporation Recombinant ricin toxin
EP0316018A3 (de) * 1985-03-29 1989-07-26 Cetus Oncology Corporation Modifikation von DNS-Sequenzen
ATE77648T1 (de) * 1985-04-15 1992-07-15 Procter & Gamble Fluessige reinigungsmittel mit einer anionischen oberflaechenaktiven verbindung, einem verstaerker und einem proteolytischen enzym.
DE3685769T2 (de) * 1985-04-15 1993-01-21 Procter & Gamble Fluessige reinigungsmittel mit einer oberflaechenaktiven verbindung, einem proteolytischen enzym und borsaeure.
IT1186750B (it) * 1985-07-10 1987-12-16 Eniricerche Spa Vettore di clonaggio,molecole di dna ricombinante,ceppi di bacillus subtilis trasformati con dette molecole e metodi per l'espressione di geni eterologhi e produzione e secrezione di proteine codificate da detti geni
DE3527913A1 (de) * 1985-08-03 1987-02-12 Henkel Kgaa Alkalische protease, verfahren zur herstellung von hybridvektoren und genetisch transformierte mikroorganismen
EP0254735B2 (de) * 1986-01-15 1998-06-17 Amgen Inc. Herstellung von ph- und wärmestabile subtilisinanaloge
US5013657A (en) * 1988-04-12 1991-05-07 Bryan Philip N Subtilisin mutations
US4908773A (en) * 1987-04-06 1990-03-13 Genex Corporation Computer designed stabilized proteins and method for producing same
IE65767B1 (en) * 1986-04-30 1995-11-15 Genencor Int Non-human carbonyl hydrolase mutants DNA sequences and vectors encoding same and hosts transformed with said vectors
EP0245051B1 (de) * 1986-05-06 1992-08-12 The Board Of Trustees Of The Leland Stanford Junior University Cytotoxische Zusammensetzung aus "Killer"-Zellen
US5364554A (en) * 1986-06-09 1994-11-15 The Clorox Company Proteolytic perhydrolysis system and method of use for bleaching
EP0260350B1 (de) * 1986-09-05 1992-02-12 Cetus Oncology Corporation Oxidationsresistente Muteine von beta-Interferon, deren Herstellung und diese Muteine enthaltende Präparate
NZ221627A (en) * 1986-09-09 1993-04-28 Genencor Inc Preparation of enzymes, modifications, catalytic triads to alter ratios or transesterification/hydrolysis ratios
BR8805646A (pt) * 1987-02-27 1989-10-17 Gist Brocades Nv Processo para amplificacao de genes estaveis em adn cromossomico de microorganismos procarioticos
EP0896062A3 (de) * 1987-02-27 1999-04-07 Genencor International, Inc. Transformation von alkolophilen Bacillus-stämmen
PT86864B (pt) * 1987-02-27 1992-05-29 Gist Brocades Nv Processo para clonagem molecular e expressao de genes que codificam para enzimas proteoliticas
EP0285123A3 (de) * 1987-04-03 1989-02-01 Stabra AG Verfahren zur vollständigen Mutagenese von Nucleinsäuren
ATE371029T1 (de) * 1987-04-06 2007-09-15 Novozymes As Regelung von elektrostatischen interaktionen an metallionen-bindungsstellen zur stabilisierung von proteinen
US4853871A (en) * 1987-04-06 1989-08-01 Genex Corporation Computer-based method for designing stablized proteins
US4914031A (en) * 1987-04-10 1990-04-03 Amgen, Inc. Subtilisin analogs
IT1228925B (it) * 1987-08-07 1991-07-10 Eniricerche Spa Procedimento per la preparazione dell'ormone della crescita umano
US5041378A (en) * 1987-08-11 1991-08-20 Cetus Corporation Procaryotic xylose isomerase muteins
US4939666A (en) * 1987-09-02 1990-07-03 Genex Corporation Incremental macromolecule construction methods
US4966846A (en) * 1987-10-01 1990-10-30 W. R. Grace & Co.-Conn. Molecular cloning and expression of a vibrio proteolyticus neutral protease gene
DK571587D0 (da) * 1987-11-02 1987-11-02 Novo Industri As Enzymatisk detergentsammensaetning
GB8727772D0 (en) * 1987-11-27 1987-12-31 Celltech Ltd Host cells
US4865983A (en) * 1987-12-04 1989-09-12 W. R. Grace & Co.-Conn. Cleaning compositions containing protease produced by vibrio and method of use
DE3877337T2 (de) * 1987-12-11 1993-08-19 Abbott Lab Verfahren zur mutagenese mittels oligonukleotid-gerichteter reparatur eines strandbruchs.
DK6488D0 (da) * 1988-01-07 1988-01-07 Novo Industri As Enzymer
PT89702B (pt) * 1988-02-11 1994-04-29 Gist Brocades Nv Processo para a preparacao de novos enzimas proteoliticos e de detergentes que os contem
US6287841B1 (en) 1988-02-11 2001-09-11 Genencor International, Inc. High alkaline serine protease
US5324653A (en) * 1988-02-11 1994-06-28 Gist-Brocades N.V. Recombinant genetic means for the production of serine protease muteins
US5246849A (en) * 1988-04-12 1993-09-21 Enzon, Inc. Thermally stable serine proteases
US5118623A (en) * 1988-05-27 1992-06-02 Solvay Enzymes, Inc. Bleach stable enzymes
CA1333777C (en) * 1988-07-01 1995-01-03 Randy M. Berka Aspartic proteinase deficient filamentous fungi
HU200796B (en) * 1988-07-14 1990-08-28 Richter Gedeon Vegyeszet Process for producing expression vectors ensuring insertion of nucleotide sequences into e. coli chromosome
US5665587A (en) * 1989-06-26 1997-09-09 Novo Nordisk A/S Modified subtilisins and detergent compositions containing same
US5658871A (en) * 1989-07-07 1997-08-19 Lever Brothers Company, Division Of Conopco, Inc. Microbial lipase muteins and detergent compositions comprising same
GB8915658D0 (en) * 1989-07-07 1989-08-23 Unilever Plc Enzymes,their production and use
NL8902010A (nl) * 1989-08-04 1991-03-01 Nl Zuivelonderzoek Inst Dna-fragment met ten minste een protease-gen, een dergelijk dna-fragment bevattende cloneringsvektor, een gastheercel getransformeerd met een dusdanig opgebouwde cloneringsvektor, alsmede de met dergelijke gastheercellen geproduceerde proteasen resp. bereide produkten zoals voedingsmiddelen.
DK0414297T3 (da) * 1989-08-11 1997-07-07 Genencor Int Effektiv fremstilling af mutantproteaser
DE4023458A1 (de) * 1989-08-31 1991-03-07 Kali Chemie Ag Neue hochalkalische proteasen
US5352603A (en) * 1989-08-31 1994-10-04 Kali-Chemie Ag Highly alkaline proteases
US6271012B1 (en) * 1989-10-11 2001-08-07 Genencor International, Inc. Protease muteins and their use in detergents
JPH0813271B2 (ja) * 1989-11-27 1996-02-14 日本ケミカルリサーチ株式会社 線溶活性蛋白質およびその製造法
US5030378A (en) * 1990-01-02 1991-07-09 The Procter & Gamble Company Liquid detergents containing anionic surfactant, builder and proteolytic enzyme
DE69133491T2 (de) * 1990-01-04 2006-07-27 Genencor International, Inc., Palo Alto Glucose-Isomerase mit geändertem pH-Optimum
IT1239733B (it) * 1990-02-23 1993-11-15 Eniricerche Spa Mutanti della proteasi neutra termostabili e mezzi e metodi per la loro preparazione
DK271490D0 (da) * 1990-11-14 1990-11-14 Novo Nordisk As Detergentkomposition
DE4037530A1 (de) * 1990-11-26 1992-05-27 Henkel Kgaa Faellungsverfahren fuer exocellulaere proteine
US5482849A (en) * 1990-12-21 1996-01-09 Novo Nordisk A/S Subtilisin mutants
US5340735A (en) * 1991-05-29 1994-08-23 Cognis, Inc. Bacillus lentus alkaline protease variants with increased stability
US5702931A (en) * 1991-07-01 1997-12-30 Berlex Laboratories, Inc. Mutagenesis methods and compositions
US5770410A (en) * 1992-01-30 1998-06-23 Genzyme Corporation Chiral synthesis with modified enzymes
JP3431146B2 (ja) * 1992-01-30 2003-07-28 ジェンザイム・リミテッド 改変した酵素によるキラル合成
US5316935A (en) * 1992-04-06 1994-05-31 California Institute Of Technology Subtilisin variants suitable for hydrolysis and synthesis in organic media
US5869644A (en) * 1992-04-15 1999-02-09 The Johns Hopkins University Synthesis of diverse and useful collections of oligonucleotidies
JPH0657299A (ja) * 1992-05-13 1994-03-01 Procter & Gamble Co:The 陰イオン界面活性剤とカルボキシレートビルダーとタンパク分解酵素とアルカノールアミノとを含有する液体洗剤
EP0651794B1 (de) * 1992-07-23 2009-09-30 Novozymes A/S MUTIERTE -g(a)-AMYLASE, WASCHMITTEL UND GESCHIRRSPÜLMITTEL
CA2155831C (en) * 1993-02-11 2009-11-10 Richard L. Antrim Oxidatively stable alpha-amylase
DK39093D0 (da) * 1993-04-01 1993-04-01 Novo Nordisk As Enzym
US6440717B1 (en) * 1993-09-15 2002-08-27 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis
US6436690B1 (en) * 1993-09-15 2002-08-20 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis wherein one or more loop regions are substituted
EP1921147B1 (de) 1994-02-24 2011-06-08 Henkel AG & Co. KGaA Verbesserte Enzyme und Detergentien damit
ES2364776T3 (es) 1994-02-24 2011-09-14 HENKEL AG & CO. KGAA Enzimas mejoradas y detergentes que las contienen.
USH1468H (en) 1994-04-28 1995-08-01 Costa Jill B Detergent compositions containing cellulase enzyme and selected perfumes for improved odor and stability
US6599730B1 (en) * 1994-05-02 2003-07-29 Procter & Gamble Company Subtilisin 309 variants having decreased adsorption and increased hydrolysis
EP0693549A1 (de) 1994-07-19 1996-01-24 The Procter & Gamble Company Feste Bleichaktivatorzusammensetzungen
NZ291984A (en) * 1994-08-11 1998-04-27 Genencor Int Bleach-containing cleaning composition comprising a mutant alpha-amylase and optionally a mutant protease
GB9416841D0 (en) 1994-08-19 1994-10-12 Finnfeeds Int Ltd An enzyme feed additive and animal feed including it
US5837516A (en) * 1995-03-03 1998-11-17 Genentech, Inc. Subtilisin variants capable of cleaving substrates containing basic residues
US5780285A (en) * 1995-03-03 1998-07-14 Genentech, Inc. Subtilisin variants capable of cleaving substrates containing dibasic residues
US6455295B1 (en) * 1995-03-08 2002-09-24 The Procter & Gamble Company Subtilisin Carlsberg variants having decreased adsorption and increased hydrolysis
US6475765B1 (en) * 1995-03-09 2002-11-05 Procter & Gamble Company Subtilisin DY variants having decreased adsorption and increased hydrolysis
IL117350A0 (en) * 1995-03-09 1996-07-23 Procter & Gamble Proteinase k variants having decreased adsorption and increased hydrolysis
US6682924B1 (en) * 1995-05-05 2004-01-27 Novozymes A/S Protease variants and compositions
ES2115338T3 (es) 1995-07-13 1998-06-16 Procter & Gamble Composicion de espumacion para envasado.
EP0753567A1 (de) 1995-07-14 1997-01-15 The Procter & Gamble Company Beim Waschen weichmachende Zusammensetzungen
DE19530816A1 (de) 1995-08-23 1997-02-27 Cognis Bio Umwelt Verwendung von mutierter Subtilisin-Protease in kosmetischen Produkten
EP0778342A1 (de) 1995-12-06 1997-06-11 The Procter & Gamble Company Waschmittelzusammensetzungen
US5858948A (en) * 1996-05-03 1999-01-12 Procter & Gamble Company Liquid laundry detergent compositions comprising cotton soil release polymers and protease enzymes
WO1997042282A1 (en) 1996-05-03 1997-11-13 The Procter & Gamble Company Detergent compositions comprising polyamine polymers with improved soil dispersancy
US5958739A (en) * 1996-06-06 1999-09-28 Genencor International Inc. Mutant α-amylase
US6165966A (en) * 1996-09-24 2000-12-26 The Procter & Gamble Company Liquid detergents containing proteolytic enzyme and protease inhibitors
JP2000506931A (ja) * 1996-09-24 2000-06-06 ザ、プロクター、エンド、ギャンブル、カンパニー タンパク質分解酵素とプロテアーゼインヒビターを含有した液体洗剤
US6180586B1 (en) 1996-09-24 2001-01-30 The Procter & Gamble Company Liquid laundry detergent compositions containing proteolytic enzyme and protease inhibitors
BR9706899A (pt) * 1996-10-29 1999-07-20 Philips Electronics Nv Receptor de televisão e processo para controlar o mesmo
CA2270593C (en) 1996-11-04 2005-06-07 Novo Nordisk A/S Subtilase variants and compositions
KR100591553B1 (ko) 1996-11-04 2006-06-19 노보자임스 에이/에스 섭틸라제 변종과 조성물
BR9714194A (pt) * 1996-12-31 2000-03-28 Procter & Gamble Composições detergentes para a lavagem de tecidos com poliamidas-poliaminas para proporcionar benefìcios na aparência aos tecidos lavados com as memas
US6156722A (en) * 1996-12-31 2000-12-05 The Procter & Gamble Company Laundry detergent compositions comprising dye fixatives
US6221825B1 (en) 1996-12-31 2001-04-24 The Procter & Gamble Company Thickened, highly aqueous liquid detergent compositions
CA2276623A1 (en) 1996-12-31 1998-07-09 Hari Achuthan Nair Thickened, highly aqueous, low cost liquid detergent compositions with aromatic surfactants
DE19717346C2 (de) * 1997-04-24 1999-05-20 Max Planck Gesellschaft DNA-Sonden, Verfahren und Kit zur Identifizierung Antibiotika-resistenter Bakterienstämme
US6080568A (en) * 1997-08-19 2000-06-27 Genencor International, Inc. Mutant α-amylase comprising modification at residues corresponding to A210, H405 and/or T412 in Bacillus licheniformis
CN1148444C (zh) 1997-08-29 2004-05-05 诺沃奇梅兹有限公司 蛋白酶变体及组合物
KR20010023468A (ko) 1997-08-29 2001-03-26 한센 핀 베네드, 안네 제헤르, 웨이콥 마리안느 프로테아제 변이체 및 조성물
US6773907B2 (en) 1997-11-21 2004-08-10 Peter Kamp Hansen Subtilase enzymes
AU1225099A (en) 1997-11-21 1999-06-15 Novo Nordisk A/S Protease variants and compositions
US6780629B2 (en) 1997-11-21 2004-08-24 Novozymes A/S Subtilase enzymes
US6423517B2 (en) 1997-12-20 2002-07-23 Genecor International, Inc. Granule containing protein and salt layered on an inert particle
DE69836098T3 (de) 1997-12-20 2014-04-30 Genencor International, Inc. In einem wirbelschichtbett hergestelltes matrixgranulat
AU2089499A (en) 1997-12-20 1999-07-12 Genencor International, Inc. Accelerated stability test
KR20010033321A (ko) 1997-12-20 2001-04-25 마가렛 에이.혼 수화된 차단 재료를 가지는 과립
US6908757B1 (en) 1998-03-26 2005-06-21 The Procter & Gamble Company Serine protease variants having amino acid deletions and substitutions
CN1214108C (zh) 1998-10-27 2005-08-10 金克克国际有限公司 基质颗粒
AU1622300A (en) 1998-11-13 2000-06-05 Douglas A. Dale Fluidized bed low density granule
DE69943346D1 (de) 1998-12-18 2011-05-19 Novozymes As Subtilase Enzyme der I-S1- und I-S2-Untergruppen mit einem zusätzlichen Aminosäurerest in einer aktiven Schleifenregion
EP1149151B1 (de) 1999-01-08 2006-03-29 Genencor International, Inc. Zusammensetzungen mit niedriger dichte und partikeln der diese enthalten
AU4392900A (en) 1999-05-20 2000-12-12 Novozymes A/S Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additionalamino acid residue between positions 127 and 128
EP1183342B2 (de) 1999-05-20 2012-06-13 Novozymes A/S Subtilase enzyme der i-s1 und i-s2 untergruppen mit mindestens einer zusätzlichen aminosäure zwischen position 128 und 129
AU4393100A (en) 1999-05-20 2000-12-12 Novozymes A/S Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additionalamino acid residue between positions 125 and 126
DE60040149D1 (de) 1999-05-20 2008-10-16 Novozymes As Subtilase-enzyme der i-s1 und i-s2 untergruppen mit wenigstens einem zusätzlichen aminosäurerest zwischen positionen 126 und 127
ATE408678T1 (de) 1999-05-20 2008-10-15 Novozymes As Subtilase enzyme der i-s1 und i-s2 untergruppen mit mindestens einer zusätzlichen aminosäure zwischen position 129 und 130
DE60040282D1 (de) 1999-05-20 2008-10-30 Novozymes As SUBTILASE ENZYME DER I-S1 UND I-S2 UNTERGRUPPEN MIT MINDESTENS EINER ZUSäTZLICHEN AMINOSäURE ZWISCHEN POSITION 132 UND 133
WO2001007578A2 (en) 1999-07-22 2001-02-01 The Procter & Gamble Company Subtilisin protease variants having amino acid substitutions in defined epitope regions
US6946128B1 (en) 1999-07-22 2005-09-20 The Procter & Gamble Company Protease conjugates having sterically protected epitope regions
JP2003505067A (ja) 1999-07-22 2003-02-12 ザ、プロクター、エンド、ギャンブル、カンパニー 特定のエピトープ領域にアミノ酸欠失及び置換を有するサブチリシンプロテアーゼの変異体
MXPA02000842A (es) 1999-07-22 2002-07-30 Procter & Gamble Conjugados de proteasa que tienen sitios de corte protegidos estericamente.
US7217554B2 (en) 1999-08-31 2007-05-15 Novozymes A/S Proteases and variants thereof
US6558939B1 (en) 1999-08-31 2003-05-06 Novozymes, A/S Proteases and variants thereof
EP2206786A1 (de) 1999-08-31 2010-07-14 Novozymes A/S Neue Proteasen und Varianten davon
WO2001018165A1 (en) * 1999-09-09 2001-03-15 The Procter & Gamble Company A detergent composition containing a protease
WO2001021499A1 (en) 1999-09-22 2001-03-29 The Procter & Gamble Company A hand-held liquid container
AU7875800A (en) 1999-10-15 2001-04-30 Genencor International, Inc. Protein-containing granules and granule formulations
US6727085B2 (en) * 1999-12-15 2004-04-27 Fanoe Tina Sejersgaard Subtilase variants having an improved wash performance on egg stains
US6960462B2 (en) 2000-02-08 2005-11-01 Dsm Ip Assets B.V Use of acid-stable subtilisin proteases in animal feed
US6855548B2 (en) 2000-02-08 2005-02-15 F. Hoffman-La Roche Ag Use of acid-stable proteases in animal feed
US6777218B1 (en) 2000-03-14 2004-08-17 Novozymes A/S Subtilase enzymes having an improved wash performance on egg stains
US6872696B2 (en) 2000-10-27 2005-03-29 Genencor International, Inc. Particle with substituted polyvinyl alcohol coating
US6803222B2 (en) 2000-11-22 2004-10-12 Kao Corporation Alkaline proteases
ATE527276T1 (de) 2001-03-23 2011-10-15 Genencor Int Proteine, die veränderte immunogene reaktionen bewirken, und verfahren zu deren herstellung und anwendung
US8076113B2 (en) 2001-04-02 2011-12-13 Danisco Us Inc. Method for producing granules with reduced dust potential comprising an antifoam agent
US7157416B2 (en) 2001-07-20 2007-01-02 Genencor International, Inc. Stabilization of enzymes
US7153820B2 (en) 2001-08-13 2006-12-26 Ecolab Inc. Solid detergent composition and method for solidifying a detergent composition
DE10153792A1 (de) 2001-10-31 2003-05-22 Henkel Kgaa Neue Alkalische Protease-Varianten und Wasch- und Reinigungsmittel enthaltend diese neuen Alkalischen Protease-Varianten
DE10162728A1 (de) 2001-12-20 2003-07-10 Henkel Kgaa Neue Alkalische Protease aus Bacillus gibsonii (DSM 14393) und Wasch-und Reinigungsmittel enthaltend diese neue Alkalische Protease
DE10162727A1 (de) 2001-12-20 2003-07-10 Henkel Kgaa Neue Alkalische Protease aus Bacillus gibsonii (DSM 14391) und Wasch-und Reinigungsmittel enthaltend diese neue Alkalische Protease
DE10163884A1 (de) 2001-12-22 2003-07-10 Henkel Kgaa Neue Alkalische Protease aus Bacillus sp. (DSM 14392) und Wasch- und Reinigungsmittel enthaltend diese neue Alkalische Protease
JP2005514026A (ja) * 2001-12-31 2005-05-19 ジェネンコー・インターナショナル・インク 免疫反応の変化を生じるプロテアーゼ、ならびにその製造および利用方法
US7332320B2 (en) 2001-12-31 2008-02-19 Genencor International, Inc. Protease producing an altered immunogenic response and methods of making and using the same
DK2339016T3 (da) 2002-03-29 2017-01-23 Danisco Us Inc Øget produktion af subtilisiner
CA2502304C (en) 2002-10-08 2013-10-01 Genencor International, Inc. Phenolic binding peptides
US7888093B2 (en) * 2002-11-06 2011-02-15 Novozymes A/S Subtilase variants
EP1594963B1 (de) 2003-02-07 2007-07-25 Novozymes A/S Proteasen
JP4819670B2 (ja) 2003-05-07 2011-11-24 ノボザイムス アクティーゼルスカブ 変異体スブチリシン酵素（サブチラーゼ）
BRPI0414959A (pt) 2003-10-10 2006-11-07 Novozymes As variante de uma protease precursora, sequência de ácido nucleico isolado, construção de ácido nucleico, vetor de expressão e célula hospedeira recombinantes, métodos para produzir a variante de protease, para melhorar o valor nutricional de uma ração animal, e para tratar proteìnas, planta transgênica, ou parte da planta, animal transgênico, não humano, ou produtos, ou elementos deste, aditivo e composição de ração animal, uso da variante de protease e/ou da composição, protease, e, estrutura 3d
WO2005040372A1 (en) 2003-10-23 2005-05-06 Novozymes A/S Protease with improved stability in detergents
EP1751264B2 (de) 2004-05-17 2013-08-28 The Procter and Gamble Company Bleichmittel mit carbohydratoxidase
CN101014704A (zh) 2004-06-21 2007-08-08 诺维信公司 蛋白酶
JP4681283B2 (ja) 2004-11-18 2011-05-11 独立行政法人海洋研究開発機構 新規な高アルカリプロテアーゼ及びその利用
JP4485341B2 (ja) 2004-12-20 2010-06-23 花王株式会社 組換え微生物
EP1700907A1 (de) 2005-03-11 2006-09-13 Unilever N.V. Flüssige bleichmittelzusammensetzung
EP1700904A1 (de) 2005-03-11 2006-09-13 Unilever N.V. Flüssige Waschmittelzusammensetzung
US7666963B2 (en) 2005-07-21 2010-02-23 Akzo Nobel N.V. Hybrid copolymers
CA2649267C (en) 2006-04-14 2014-08-12 Genencor International, Inc. One-step treatment of textiles
EP2013339B1 (de) 2006-04-20 2013-09-11 Novozymes A/S Savinase-varianten mit verbesserter waschleistung gegen eiflecken
US8674021B2 (en) 2006-07-21 2014-03-18 Akzo Nobel N.V. Sulfonated graft copolymers
US20080020961A1 (en) 2006-07-21 2008-01-24 Rodrigues Klin A Low Molecular Weight Graft Copolymers
US8093200B2 (en) 2007-02-15 2012-01-10 Ecolab Usa Inc. Fast dissolving solid detergent
JP5427776B2 (ja) 2007-04-30 2014-02-26 ダニスコ・ユーエス・インク、ジェネンコー・ディビジョン メタロプロテアーゼ洗剤調合剤を安定化するためのタンパク質の加水分解物の使用
SG148934A1 (en) 2007-06-11 2009-01-29 Novozymes As A process for combined biopolishing and bleach clean-up
WO2009009493A2 (en) 2007-07-09 2009-01-15 THE UNITED STATES OF AMERICA, as represented by THE SECRETARY, DEPARTEMENT OF HEALTH AND HUMAN SERVICES Aegyptin and uses thereof
CN101827925B (zh) 2007-10-18 2012-11-28 埃科莱布有限公司 经压制的蜡状固体清洁组合物和它们的制造方法
MX2010004372A (es) * 2007-10-31 2010-05-20 Danisco Us Inc Uso y elaboracion de metaloproteasas neutras estables en citrato.
CA2714751C (en) 2008-02-14 2020-05-05 Danisco Us Inc. Small enzyme-containing granules
US8293697B2 (en) 2009-03-18 2012-10-23 The Procter & Gamble Company Structured fluid detergent compositions comprising dibenzylidene sorbitol acetal derivatives
US8153574B2 (en) 2009-03-18 2012-04-10 The Procter & Gamble Company Structured fluid detergent compositions comprising dibenzylidene polyol acetal derivatives and detersive enzymes
WO2010138347A1 (en) 2009-05-26 2010-12-02 The Procter & Gamble Company Aqueous liquid composition for pre-treating soiled dishware
DE102009027045A1 (de) * 2009-06-19 2010-12-30 Henkel Ag & Co. Kgaa Neue Proteasen und Mittel enthaltend diese Proteasen
WO2011005623A1 (en) 2009-07-09 2011-01-13 The Procter & Gamble Company Laundry detergent composition comprising low level of bleach
US20110009307A1 (en) 2009-07-09 2011-01-13 Alan Thomas Brooker Laundry Detergent Composition Comprising Low Level of Sulphate
MX341475B (es) 2009-07-31 2016-08-19 Akzo Nobel N V * Composiciones de copolimero hibrido para aplicaciones de cuidado personal.
US9464261B2 (en) 2010-05-14 2016-10-11 The Sun Products Corporation Polymer-containing cleaning compositions and methods of production and use thereof
US20130224757A1 (en) * 2010-08-19 2013-08-29 Novozymes A/S Induced sporulation screening method
KR101890147B1 (ko) 2011-05-20 2018-08-21 에코랍 유에스에이 인코퍼레이티드 식기세척을 위한 교대 알칼리/산 시스템에서의 비-포스페이트 세제 및 비-인산
EP2766462B1 (de) 2011-05-20 2019-08-28 Ecolab USA Inc. Säureformulierungen zur verwendung in einem waschsystem
US8679366B2 (en) 2011-08-05 2014-03-25 Ecolab Usa Inc. Cleaning composition containing a polysaccharide graft polymer composition and methods of controlling hard water scale
US8636918B2 (en) 2011-08-05 2014-01-28 Ecolab Usa Inc. Cleaning composition containing a polysaccharide hybrid polymer composition and methods of controlling hard water scale
US8841246B2 (en) 2011-08-05 2014-09-23 Ecolab Usa Inc. Cleaning composition containing a polysaccharide hybrid polymer composition and methods of improving drainage
US8853144B2 (en) 2011-08-05 2014-10-07 Ecolab Usa Inc. Cleaning composition containing a polysaccharide graft polymer composition and methods of improving drainage
EP2773321B1 (de) 2011-11-04 2015-09-09 Akzo Nobel Chemicals International B.V. Dendrit-pfropfcopolymere und verfahren zu ihrer herstellung
MX2014005094A (es) 2011-11-04 2014-08-08 Akzo Nobel Chemicals Int Bv Copolimeros de dendrita hibridos, composiciones de los mismos y metodos para producirlos.
EP2791301B1 (de) 2011-12-13 2017-10-04 Ecolab USA Inc. Konzentrierte artikelwaschzusammensetzungen und verfahren dafür
WO2014007229A1 (ja) * 2012-07-06 2014-01-09 東洋紡株式会社 改変型アルカリホスファターゼ
MX2015000924A (es) 2012-07-26 2015-04-10 Procter & Gamble Composiciones liquidas de limpieza con ph bajo con enzimas.
US8945314B2 (en) 2012-07-30 2015-02-03 Ecolab Usa Inc. Biodegradable stability binding agent for a solid detergent
US9745543B2 (en) 2012-09-10 2017-08-29 Ecolab Usa Inc. Stable liquid manual dishwashing compositions containing enzymes
CN113105950A (zh) 2012-10-04 2021-07-13 艺康美国股份有限公司 用于洗衣和其他硬表面清洁的预浸泡工艺
US9139458B2 (en) 2013-03-15 2015-09-22 Janet Angel Compositions and methods of use
BR112016005143B1 (pt) 2013-09-09 2021-10-19 Ecolab Usa Inc Composição detergente concentrada, solução aquosa e uso de uma composição detergente concentrada
EP3561034B1 (de) 2013-09-09 2022-07-13 Ecolab USA Inc. Verfahren zur herstellung von portionierten waschmitteltabletten mit synergistischer fleckenentfernung durch neuartige chelatbildnerkombination
EP3060640B2 (de) 2013-10-24 2026-02-18 Ecolab USA Inc. Verfahren zur erdentfernung von oberflächen
US9365805B2 (en) 2014-05-15 2016-06-14 Ecolab Usa Inc. Bio-based pot and pan pre-soak
US11103118B2 (en) 2014-08-01 2021-08-31 Ecolab Usa Inc. Method of manual surface cleaning using cleaning textiles and of washing said cleaning textiles
US10577571B2 (en) 2016-11-08 2020-03-03 Ecolab Usa Inc. Non-aqueous cleaner for vegetable oil soils
WO2020099491A1 (en) 2018-11-14 2020-05-22 Novozymes A/S Oral care composition comprising a polypeptide having dnase activity
WO2021022045A1 (en) 2019-07-31 2021-02-04 Ecolab Usa Inc. Personal protective equipment free delimer compositions
US11873465B2 (en) 2019-08-14 2024-01-16 Ecolab Usa Inc. Methods of cleaning and soil release of highly oil absorbing substrates employing optimized extended chain nonionic surfactants
CN110760466B (zh) * 2019-11-18 2021-04-20 山东隆科特酶制剂有限公司 一株产耐高温中性蛋白酶的枯草芽孢杆菌及其应用
JP2023534927A (ja) 2020-07-06 2023-08-15 エコラボ ユーエスエー インコーポレイティド 構造化アルコキシル化シロキサンを含む起泡性アルコール／水混合組成物
WO2022010893A1 (en) 2020-07-06 2022-01-13 Ecolab Usa Inc. Foaming mixed alcohol/water compositions comprising a combination of alkyl siloxane and a hydrotrope/solubilizer
EP4176031A1 (de) 2020-07-06 2023-05-10 Ecolab USA Inc. Peg-modifizierte rizinusöl-basierte zusammensetzungen zur mikroemulgierung und entfernung von mehreren ölhaltigen böden
JP2023539234A (ja) 2020-08-24 2023-09-13 ノボザイムス アクティーゼルスカブ フルクタナーゼを含む口腔ケア組成物
US20240247209A1 (en) 2021-05-18 2024-07-25 Nouryon Chemicals International B.V. Polyester polyquats in cleaning applications
EP4341317A1 (de) 2021-05-20 2024-03-27 Nouryon Chemicals International B.V. Hergestellte polymere mit veränderter oligosaccharid- oder polysaccharid-funktionalität oder verringerter oligosaccharidverteilung, verfahren zu ihrer herstellung, zusammensetzungen damit und verfahren zu ihrer verwendung
US20240287409A1 (en) 2021-06-30 2024-08-29 Nouryon Chemicals International B.V. Chelate-amphoteric surfactant liquid concentrates and use thereof in cleaning applications
WO2023057367A1 (en) 2021-10-08 2023-04-13 Unilever Ip Holdings B.V. Laundry composition
US20240026248A1 (en) 2022-07-20 2024-01-25 Ecolab Usa Inc. Novel nonionic extended surfactants, compositions and methods of use thereof

Family Cites Families (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US362957A (en) * 1887-05-17 haig-ht
US3557002A (en) * 1967-11-15 1971-01-19 Procter & Gamble Stabilized aqueous enzyme preparation
US3558498A (en) 1967-11-29 1971-01-26 Procter & Gamble Granular detergent composition containing enzymes and environmental control components
GB1240058A (en) * 1968-04-12 1971-07-21 Procter & Gamble Enzyme-containing detergent compositions
US3623957A (en) 1970-01-21 1971-11-30 Baxter Laboratories Inc Preparation of microbial alkaline protease by fermentation with bacillus subtilis, variety licheniformis
BE785666A (fr) 1971-07-01 1973-01-02 Procter & Gamble Procede pour la production de compositions enzymatiques en granules
BE792982A (en) 1971-12-20 1973-06-19 Procter & Gamble Europ Proteolytic enzymes detergent - contg cationic and anionic surfactants
US4011169A (en) 1973-06-29 1977-03-08 The Procter & Gamble Company Stabilization and enhancement of enzymatic activity
IT1106254B (it) 1976-03-08 1985-11-11 Procter & Gamble Europ Composizione detergente liquida contenente enzimi
US4090973A (en) 1976-06-24 1978-05-23 The Procter & Gamble Company Method for making stable detergent compositions
DE2633601A1 (de) 1976-07-27 1978-02-02 Henkel Kgaa Fluessiges, als wasch- und reinigungsmittel verwendbares, enzymhaltiges konzentrat
GB1603640A (en) 1977-07-20 1981-11-25 Gist Brocades Nv Enzyme particles
US4261868A (en) 1979-08-08 1981-04-14 Lever Brothers Company Stabilized enzymatic liquid detergent composition containing a polyalkanolamine and a boron compound
US4302544A (en) * 1979-10-15 1981-11-24 University Of Rochester Asporogenous mutant of B. subtilis for use as host component of HV1 system
US4318818A (en) 1979-11-09 1982-03-09 The Procter & Gamble Company Stabilized aqueous enzyme composition
JPS5950280B2 (ja) 1980-10-24 1984-12-07 花王株式会社 酵素入り漂白剤組成物
JPS5786299A (en) * 1980-11-19 1982-05-29 Dai Ichi Seiyaku Co Ltd Plasmid and its preparation
CA1170202A (en) * 1981-01-15 1984-07-03 Susan Mickel Process for cloning the gene coding for a thermostable alpha-amylase into escherichia coli and bacillus subtilis
US4404128A (en) 1981-05-29 1983-09-13 The Procter & Gamble Company Enzyme detergent composition
DE3122216A1 (de) * 1981-06-04 1982-12-23 Boehringer Mannheim Gmbh, 6800 Mannheim Verfahren zur herstellung eines mikroorganismus, welcher (alpha)-galactosidase, aber keine invertase bildet, so erhaltener mikroorganismus und seine verwendung
EP0080748B1 (de) 1981-11-13 1985-07-10 Unilever N.V. Enzymatische flüssige Reinigungsmittel-Zusammensetzung
JPS58162291A (ja) * 1982-03-23 1983-09-26 Res Dev Corp Of Japan 耐熱性プロテア−ゼの製造法
IL68366A (en) * 1982-04-15 1990-08-31 Genentech Inc Process for expressing a gene encoding a protein comprising the enzymatic portion of human urokinase in a microorganism or cell culture
JPS5959190A (ja) * 1982-09-29 1984-04-04 Agency Of Ind Science & Technol プロテア−ゼの製造法
DK135983D0 (da) * 1983-03-25 1983-03-25 Novo Industri As Maltogen amylaseenzymprodukt og fremgangsmade til dets fremstilling og anvendelse
IE81141B1 (en) 1983-06-24 2000-04-05 Genencor Int Procaryotic carbonyl hydrolases

Also Published As

Publication number Publication date
EP0130756A1 (de) 1985-01-09
HK99293A (en) 1993-09-30
JPH06315378A (ja) 1994-11-15
DK82393D0 (da) 1993-07-08
EP0247647A1 (de) 1987-12-02
EP0357157A3 (de) 1990-03-28
EP0357157A2 (de) 1990-03-07
NZ208612A (en) 1991-09-25
EP0130756B2 (de) 2000-06-28
DK82393A (da) 1993-07-08
EP0246678A1 (de) 1987-11-25
DK175278B1 (da) 2004-08-02
DE3486139D1 (de) 1993-06-03
DK82293D0 (da) 1993-07-08
JPS6070075A (ja) 1985-04-20
JP2889095B2 (ja) 1999-05-10
ATE60797T1 (de) 1991-02-15
JP2594533B2 (ja) 1997-03-26
HK75393A (en) 1993-08-06
DE3484019D1 (de) 1991-02-28
DK305984D0 (da) 1984-06-22
DE3484079D1 (de) 1991-03-14
DK82293A (da) 1993-07-08
DK175768B1 (da) 2005-02-14
JPH06319534A (ja) 1994-11-22
EP0246678B1 (de) 1993-04-28
DE3486139T2 (de) 1993-11-11
DK305984A (da) 1985-02-18
IE81141B1 (en) 2000-04-05
EP0247647B1 (de) 1991-01-23
ATE60356T1 (de) 1991-02-15
IE841567L (en) 1984-12-24
EP0130756B1 (de) 1991-02-06

Similar Documents

Publication Publication Date Title
EP0130756B1 (de) Prokaryotische Carbonyl-Hydrolasen, Verfahren, DNA, Vektoren und transformierte Wirte zu ihrer Herstellung und diese Hydrolasen enthaltende Detergenszusammensetzungen
US4760025A (en) Modified enzymes and methods for making same
US5264366A (en) Protease deficient bacillus
US5310675A (en) Procaryotic carbonyl hydrolases
US5346823A (en) Subtilisin modifications to enhance oxidative stability
US6465235B1 (en) Non-human carbonyl hydrolase mutants, DNA sequences and vectors encoding same and hosts transformed with said vectors
EP0396608B1 (de) Mutierte subtilisingene
US5763257A (en) Modified subtilisins having amino acid alterations
EP0328229B2 (de) Proteolytische Enzyme und ihre Verwendung in Waschmitteln
US5411873A (en) Process for producing heterologous polypeptides
CA1339100C (en) Molecular cloning and expression of genes encoding proteolytic enzymes
US20040014175A1 (en) Efficient production of mutant proteases
CA1339893C (en) Procaryotic carbonyl hydrolases
NZ236052A (en) Bacillus incapable of excreting neutral protease or subtilisin, vector and production of amylase

Legal Events

Date Code Title Description
PE Patent expired

Effective date: 20040621

PE Patent expired