ES2226609T1 - Variantes de amilasa. - Google Patents

Variantes de amilasa.

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Publication number
ES2226609T1
ES2226609T1 ES96900894T ES96900894T ES2226609T1 ES 2226609 T1 ES2226609 T1 ES 2226609T1 ES 96900894 T ES96900894 T ES 96900894T ES 96900894 T ES96900894 T ES 96900894T ES 2226609 T1 ES2226609 T1 ES 2226609T1
Authority
ES
Spain
Prior art keywords
amino acid
variant
amylase
seq
bacillus
Prior art date
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Granted
Application number
ES96900894T
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English (en)
Other versions
ES2226609T3 (es
Inventor
Henrik Bisgard-Frantzen
Allan Svendsen
Torben Vedel Borchert
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Novozymes AS
Original Assignee
Novozymes AS
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Application filed by Novozymes AS filed Critical Novozymes AS
Publication of ES2226609T1 publication Critical patent/ES2226609T1/es
Application granted granted Critical
Publication of ES2226609T3 publication Critical patent/ES2226609T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38618—Protease or amylase in liquid compositions only
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38609—Protease or amylase in solid compositions only
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38672—Granulated or coated enzymes
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38681—Chemically modified or immobilised enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12C—BEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
    • C12C5/00—Other raw materials for the preparation of beer
    • C12C5/004—Enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01001—Alpha-amylase (3.2.1.1)
    • D—TEXTILES; PAPER
    • D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
    • D06L—DRY-CLEANING, WASHING OR BLEACHING FIBRES, FILAMENTS, THREADS, YARNS, FABRICS, FEATHERS OR MADE-UP FIBROUS GOODS; BLEACHING LEATHER OR FURS
    • D06L1/00—Dry-cleaning or washing fibres, filaments, threads, yarns, fabrics, feathers or made-up fibrous goods
    • D06L1/12—Dry-cleaning or washing fibres, filaments, threads, yarns, fabrics, feathers or made-up fibrous goods using aqueous solvents
    • D06L1/14—De-sizing
    • D—TEXTILES; PAPER
    • D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
    • D21C—PRODUCTION OF CELLULOSE BY REMOVING NON-CELLULOSE SUBSTANCES FROM CELLULOSE-CONTAINING MATERIALS; REGENERATION OF PULPING LIQUORS; APPARATUS THEREFOR
    • D21C5/00—Other processes for obtaining cellulose, e.g. cooking cotton linters ; Processes characterised by the choice of cellulose-containing starting materials
    • D21C5/005—Treatment of cellulose-containing material with microorganisms or enzymes
    • D—TEXTILES; PAPER
    • D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
    • D21C—PRODUCTION OF CELLULOSE BY REMOVING NON-CELLULOSE SUBSTANCES FROM CELLULOSE-CONTAINING MATERIALS; REGENERATION OF PULPING LIQUORS; APPARATUS THEREFOR
    • D21C5/00—Other processes for obtaining cellulose, e.g. cooking cotton linters ; Processes characterised by the choice of cellulose-containing starting materials
    • D21C5/02—Working-up waste paper
    • D21C5/022—Chemicals therefor
    • D—TEXTILES; PAPER
    • D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
    • D21H—PULP COMPOSITIONS; PREPARATION THEREOF NOT COVERED BY SUBCLASSES D21C OR D21D; IMPREGNATING OR COATING OF PAPER; TREATMENT OF FINISHED PAPER NOT COVERED BY CLASS B31 OR SUBCLASS D21G; PAPER NOT OTHERWISE PROVIDED FOR
    • D21H17/00—Non-fibrous material added to the pulp, characterised by its constitution; Paper-impregnating material characterised by its constitution
    • D21H17/005—Microorganisms or enzymes
    • D—TEXTILES; PAPER
    • D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
    • D21H—PULP COMPOSITIONS; PREPARATION THEREOF NOT COVERED BY SUBCLASSES D21C OR D21D; IMPREGNATING OR COATING OF PAPER; TREATMENT OF FINISHED PAPER NOT COVERED BY CLASS B31 OR SUBCLASS D21G; PAPER NOT OTHERWISE PROVIDED FOR
    • D21H17/00—Non-fibrous material added to the pulp, characterised by its constitution; Paper-impregnating material characterised by its constitution
    • D21H17/20—Macromolecular organic compounds
    • D21H17/21—Macromolecular organic compounds of natural origin; Derivatives thereof
    • D21H17/24—Polysaccharides
    • D21H17/28—Starch
    • D—TEXTILES; PAPER
    • D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
    • D21C—PRODUCTION OF CELLULOSE BY REMOVING NON-CELLULOSE SUBSTANCES FROM CELLULOSE-CONTAINING MATERIALS; REGENERATION OF PULPING LIQUORS; APPARATUS THEREFOR
    • D21C5/00—Other processes for obtaining cellulose, e.g. cooking cotton linters ; Processes characterised by the choice of cellulose-containing starting materials
    • D21C5/02—Working-up waste paper
    • D21C5/025—De-inking
    • D21C5/027—Chemicals therefor
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00—Technologies for solid waste management
    • Y02W30/50—Reuse, recycling or recovery technologies
    • Y02W30/64—Paper recycling

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Textile Engineering (AREA)
  • Food Science & Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Toxicology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Detergent Compositions (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

SE DESCRIBE UNA VARIANTE DE UNA {AL} POSEYENDO LA {AL} ELECCIONADA ENTRE LAS SECUENCIAS DE AMINOACIDOS QUE APARECEN EN LA SEC ID N CTIVAMENTE; O (II) PRESENTA AL MENOS UN 80 % DE HOMOLOGIA CON UNA O MAS DE ESTAS SECUENCIAS DE AMINOACIDOS; Y/O PRESENTA UNA REACTIVIDAD CRUZADA INMUNOLOGICA CON UN ANTICUERPO PRODUCIDO CONTRA UNA {AL} AMINOACIDOS; Y/O ESTA CODIFICADA POR UNA SECUENCIA DE ADN QUE SE HIBRIDA CON LA MISMA SONDA QUE LA SECUENCIA DE ADN QUE CODIFICA PARA UNA {AL} DE AMINOACIDOS; EN DICHA VARIANTE: (A) AL MENOS UN RESTO AMINOACIDO DE LA {AL} B) AL MENOS UN RESTO AMINOACIDO DE LA {AL} HA SIDO SUSTITUIDO POR UN RESTO AMINOACIDO DIFERENTE; Y/O (C) AL MENOS UN RESTO AMINOACIDO SE HA INSERTADO EN RELACION A LA {AL} AMILASA PARENTAL; MAYOR TERMOESTABILIDAD; MAYOR ESTABILIDAD FRENTE A LA OXIDACION; Y MENOR DEPENDENCIA DE CA{SUP,2+}; CON LA CONDICION DE QUE LA SECUENCIA DE AMINOACIDOS ES UNA VARIANTE QUE NO ES IDENTICA A CUALQUIERA DE LAS SECUENCIAS DE AMINOACIDOS QUE APARECEN EN LA SEC ID N3 Y SEC ID N

Claims (29)

1. Variante de una \alpha-amilasa madre, \alpha-amilasa madre la cual (i) tiene una secuencia de aminoácidos seleccionada de las secuencias de aminoácidos mostradas en SEC ID No.1, SEC ID No.2, SEC ID No.3, y SEC ID No.7, respectivamente; o (ii) muestra al menos un 80% de homología con una o más de dichas secuencias de aminoácidos; y/o muestra reactividad cruzada inmunológica con un anticuerpo preparado contra un \alpha-amilasa que tiene una de dichas secuencias de aminoácidos; y/o está codificada por una secuencia de ADN que híbrida con la misma sonda que una secuencia de ADN que codifica una \alpha-amilasa que tiene una de dichas secuencias de aminoácidos; variante en la cual:
(a)
al menos un residuo aminoácido de dicha \alpha-amilasa madre ha sido eliminado; y/o
(b)
al menos un residuo aminoácido de dicha \alpha-amilasa madre ha sido sustituido por un residuo aminoácido diferente; y/o
(c)
al menos un residuo aminoácido ha sido insertado en relación con dicha \alpha-amilasa madre;
dicha variante tiene actividad \alpha-amilasa y exhibe al menos una de las siguientes propiedades en relación a dicha \alpha-amilasa madre: una mayor termoestabilidad; una mayor estabilidad a la oxidación; y una menor dependencia al Ca^{2+};
con la condición de que la secuencia de aminoácidos de dicha variante no sea idéntica a ninguna de las secuencias de aminoácidos mostradas en SEC ID No.1, SEC ID No.2, SEC ID No.3 y SEC ID No.7, respectivamente.
2. Variante según la reivindicación 1, en la que al menos un residuo aminoácido oxidable de dicha \alpha-amilasa madre ha sido eliminado o ha sido sustituido por un residuo aminoácido diferente que es menos susceptible a la oxidación que dicho residuo aminoácido oxidable.
3. Variante según la reivindicación 2, en la que dicho residuo aminoácido oxidable está seleccionado del grupo que se compone de metionina, triptófano, cisteína y tirosina.
4. Variante según la reivindicación 2 ó 3, en la que dicho residuo aminoácido oxidable es una metionina que es, o que es equivalente a, M9, M10, M105, M202, M208, M261, M309, M382, M430 o M440 de la secuencia de aminoácidos mostrada en la SEC ID No.1.
5. Variante según la reivindicación 4, que comprende una sustitución de metionina que es, o que es equivalente a, una de las sustituciones siguientes de la secuencia de aminoácidos mostrada en la SEC ID No. 1: M9L; M1OL; M105L; M202L,T,F,I,V; M208L; M261L; M309L; M382I; M430L; M440L.
6. Variante según cualquiera de las reivindicaciones 3-5, en la que uno de dichos residuos de metionina ha sido sustituido por treonina.
7. Variante según cualquiera de las reivindicaciones precedentes, en la que al menos un aminoácido que es, o que es equivalente a, F180, R181, G182, T183, G184 o K185 de la secuencia de aminoácidos mostrada en la SEC ID No. 1 ha sido eliminado.
8. Variante según la reivindicación 7, en la que los aminoácidos eliminadosson, o son equivalentes a, cualquier par de dichos residuos aminoácidos.
9. Variante según la reivindicación 8, en la que las eliminaciones son, o son equivalentes a, R181* + G182*; o T183* + G184*.
10. Variante según cualquiera de las reivindicaciones precedentes, la cual comprende una sustitución de un aminoácido que es, o que es equivalente a, una de las siguientes sustituciones de la secuencia de aminoácidos mostrada en la SEC ID No. 1: K269R; P260E; R124P; M105F,I,L,V; M208F,W,Y; L2171; V2061,L,F.
11. Variante según cualquiera de las reivindicaciones precedentes, la cual comprende una sustitución de un aminoácido que es, o que es equivalente a, una de las siguientes sustituciones de la secuencia de aminoácidos mostrada en la SEC ID No. 1: Y243F; K108R; K179R; K239R; K242R: K269R; D163N; D188N; D192N; D199N; D205N; D207N; D209N; E190Q; E194Q; N106D.
12. Construcción de ADN que comprende una secuencia de ADN que codifica una variante de \alpha-amilasa según cualquiera de las reivindicaciones 1-11.
13. Vector de expresión recombinante que lleva una construcción de ADN según la reivindicación 12.
14. Célula que es transformada con una construcción de ADN según la reivindicación 12 o un vector según la reivindicación 13.
15. Célula según la reivindicación 14, que es un microorganismo.
16. Célula según la reivindicación 15, que es una bacteria o un hongo.
17. Célula según la reivindicación 16, que es una bacteria grampositiva como Bacillus subtilis, Bacillus licheniformis, Bacillus lentus, Bacillus brevis, Bacillus stearothermophilus, Bacillus alkalophilus, Bacillus amyloliquefaciens, Bacillus coagulans, Bacillus circulans, Bacillus lautus, Bacillus thuringiensis o Streptomyces lividans o Streptomyces murinus, o una bacteria gramnegativa como E.coli.
18. Método para producir un variante de \alpha-amilasa según cualquiera de las reivindicaciones 1-11, en el cual una célula según cualquiera de las reivindicaciones 14-17 es cultivada en condiciones que conducen a la producción de la variante de \alpha-amilasa, y la variante de \alpha-amilasa es posteriormente recuperada del cultivo.
19. Uso de una variante de \alpha-amilasa según cualquiera de las reivindicaciones 1-11 para el lavado y/o el lavado de la vajilla.
20. Aditivo detergente que comprende una variante de \alpha-amilasa según cualquiera de las reivindicaciones 1-11, opcionalmente en forma de un granulado no espolvoreado, un líquido estabilizado o una enzima protegida.
21. Aditivo detergente según la reivindicación 20, que comprende 0.02-200mg de proteína de enzima por gramo de aditivo.
22. Aditivo detergente según la reivindicación 20 ó 21, que además comprende otra enzima como una proteasa, una lipasa, una peroxidasa, otra enzima amilolítica y/o una celulasa.
23. Composición de detergente que comprende un variante de \alpha-amilasa según cualquiera de las reivindicaciones 1-11, y un agente tensioactivo.
24. Composición de detergente según la reivindicación 23, que además comprende otra enzima como una proteasa, una lipasa, una peroxidasa, otra enzima amilolítica y/o una celulasa.
25. Composición de detergente manual o automático para la vajilla que comprende una variante de \alpha-amilasa según cualquiera de las reivindicaciones 1-11, y un agente tensioactivo.
26. Composición de detergente para la vajilla según la reivindicación 25, que además comprende otra enzima como una proteasa, una lipasa, una peroxidasa, otra enzima amilolítica y/o una celulasa.
27. Composición para el lavado de ropa manual o automático que comprende una variante de \alpha-amilasa según cualquiera de las reivindicaciones 1-11, y un agente tensioactivo.
28. Composición para el lavado de ropa según la reivindicación 27, que además comprende otra enzima como una proteasa, una lipasa, una peroxidasa. una enzima amilolítica y/o una celulasa.
29. Uso de una variante de \alpha-amilasa según cualquiera de las reivindicaciones 1-11 para el desencolado textil.
ES96900894T 1995-02-03 1996-02-05 Variantes de amilasa. Expired - Lifetime ES2226609T3 (es)

Applications Claiming Priority (12)

Application Number Priority Date Filing Date Title
DK12695 1995-02-03
DK12695 1995-02-03
DK126/95 1995-02-03
DK33695 1995-03-29
DK336/95 1995-03-29
DK33695 1995-03-29
DK1097/95 1995-09-29
DK109795 1995-09-29
DK109795 1995-09-29
DK112195 1995-10-06
DK112195 1995-10-06
DK1121/95 1995-10-06

Publications (2)

Publication Number Publication Date
ES2226609T1 true ES2226609T1 (es) 2005-04-01
ES2226609T3 ES2226609T3 (es) 2007-04-01

Family

ID=27439257

Family Applications (2)

Application Number Title Priority Date Filing Date
ES96900894T Expired - Lifetime ES2226609T3 (es) 1995-02-03 1996-02-05 Variantes de amilasa.
ES05002888T Expired - Lifetime ES2269020T3 (es) 1995-02-03 1996-02-05 Variantes dee amilasa.

Family Applications After (1)

Application Number Title Priority Date Filing Date
ES05002888T Expired - Lifetime ES2269020T3 (es) 1995-02-03 1996-02-05 Variantes dee amilasa.

Country Status (15)

Country Link
US (2) US20110177990A1 (es)
EP (6) EP2465930A3 (es)
JP (2) JP4116074B2 (es)
CN (2) CN100535109C (es)
AR (3) AR000862A1 (es)
AT (2) ATE360026T1 (es)
AU (1) AU4483396A (es)
BR (1) BR9607735A (es)
CA (2) CA2821537C (es)
DE (4) DE05002888T1 (es)
DK (2) DK1538155T3 (es)
ES (2) ES2226609T3 (es)
MX (1) MX9705727A (es)
PT (1) PT1538155E (es)
WO (1) WO1996023873A1 (es)

Families Citing this family (890)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MX9705906A (es) 1995-02-03 1997-10-31 Novo Nordisk As Un metodo para diseñar mutantes de alfa-amilasa con propiedades predeterminadas.
US6440716B1 (en) 1995-02-03 2002-08-27 Novozymes A/S α-amylase mutants
EP0756001A1 (en) * 1995-07-24 1997-01-29 The Procter & Gamble Company Detergent compositions comprising specific amylase and a specific surfactant system
JP2000505502A (ja) * 1996-03-07 2000-05-09 ザ、プロクター、エンド、ギャンブル、カンパニー 改良アミラーゼを含んでなる洗剤組成物
US5763385A (en) * 1996-05-14 1998-06-09 Genencor International, Inc. Modified α-amylases having altered calcium binding properties
US6197070B1 (en) 1996-05-15 2001-03-06 The Procter & Gamble Company Detergent compositions comprising alpha combination of α-amylases for malodor stripping
JPH11511785A (ja) * 1996-05-15 1999-10-12 ザ、プロクター、エンド、ギャンブル、カンパニー 改良アミラーゼ、セルラーゼおよびカチオン性界面活性剤を含んでなる洗剤組成物
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CN100535109C (zh) 2009-09-02
EP1752525A1 (en) 2007-02-14
ES2269020T3 (es) 2007-10-01
ATE335077T1 (de) 2006-08-15
AU4483396A (en) 1996-08-21
DE69636408D1 (en) 2006-09-14
CA2211405A1 (en) 1996-08-08
BR9607735A (pt) 1998-07-14
EP2465930A2 (en) 2012-06-20
EP2455465A3 (en) 2012-08-22
CA2821537A1 (en) 1996-08-08
AR053946A2 (es) 2007-05-23
DE05002888T1 (de) 2007-02-08
CA2211405C (en) 2013-10-01
JP2008178412A (ja) 2008-08-07
ES2269020T1 (es) 2007-04-01
ATE360026T1 (de) 2007-05-15
DK1538155T3 (da) 2007-07-02
EP0815208B1 (en) 2006-08-02
EP2455468A3 (en) 2013-01-23
US20110177990A1 (en) 2011-07-21
DE69637036T2 (de) 2008-04-30
JPH11503003A (ja) 1999-03-23
EP1538155A3 (en) 2005-06-15
DE69636408T2 (de) 2007-07-26
US20150080283A1 (en) 2015-03-19
EP1538155A2 (en) 2005-06-08
JP4116074B2 (ja) 2008-07-09
DE69637036D1 (de) 2007-05-31
CN1624124A (zh) 2005-06-08
DE96900894T1 (de) 2005-03-31
EP1538155B1 (en) 2007-04-18

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